Rh5BG205800

Integral membrane protein DUF92

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008669
Physical Location & Seq
Reverse (-)
23346720 .. 23350831
4112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG205800.1

Sequence Viewer

Length: 765 bp
ATGTTATTGCCATCCACCTCTAAAATCCAACCTCAACCAACAATTTTGGGTCAAGAGTCCGGAATAAACATTGCATTTTCTTTGGCTTCACACACACTCAAACCTCAAAACCAAATGGCATTGTCAACACTCTTGCACATCAAACCACCTCCTCCGTTTCCTTCACTAAAATCCCACCGCTCCTTCTTCCCAATTCTCCCCCCAAAAATCTCAATCCCAATCCCAATCCAAAACCCTAAACCCATCAAACACTTCACTATGCAGAGGCCTCATGCATCCACCACTGTGCACAGTGCTGTCTCCGGCGCCTTCACCCTGATCCGATCCTCGCCCGCCACGTGGCAGTCGGCAATCCTCAGCAACCTCCTGATCTTCGTTCTGGGCTCTCCGATTTTGGTTTCTGGGCTCTCCCTTTCTGGCATTGCTGCTGCGTTTTTGCTCGGAACTCTCACTTGGCGCGCTTTTGGGCCATCTGGGTTTCTCTTGGTGGCAACCTACTTCATCATTGGCACGGCGGTGACAAAGGTGAGAATGGCACAAAAGGAGGCTGAGGGGGTAGCTGAGAAGAGAAAAGGAAGGAGAGGCCCAGGCAGTGTTATTGGATCCAGTGCTGCTGGTTGCATTTGCGCTTTTCTCACGATATTTCTAGTAGGAGGAAAGGCATATTCCCGGCTCTGGCAACTAGGGTTTGTTGCCAGTTTCTGTACTAAGTTGAGTGACACTGTCTCAAGTGAGGTGGGAAAGGCATATGGGAAAACAACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF92

Protein Analysis

254

Amino Acids

27.0

Weight (kDa)

10.52

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF92 PF01940 125 - 253 8.8e-31 Integral membrane protein DUF92
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 180
AccII CGCG 1 cut(s) 459
AccIII TCCGGA 1 cut(s) 59
AciI CCGC 3 cut(s) 178, 333, 515
AclWI GGATC 4 cut(s) 313, 318, 597, 610
AcvI CACGTG 1 cut(s) 339
AcyI GRCGYC 1 cut(s) 306
AfaI GTAC 1 cut(s) 706
AfiI CCNNNNNNNGG 2 cut(s) 339, 675
AjnI CCWGG 1 cut(s) 586
AjuI GAANNNNNNNTTGG 2 cut(s) 436, 468
AleI CACNNNNGTG 2 cut(s) 284, 515
AluBI AGCT 1 cut(s) 560
AluI AGCT 1 cut(s) 560
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 2 cut(s) 304, 730
Alw44I GTGCAC 1 cut(s) 287
AlwI GGATC 4 cut(s) 313, 318, 597, 610
AlwNI CAGNNNCTG 1 cut(s) 702
Aor13HI TCCGGA 1 cut(s) 59
AoxI GGCC 3 cut(s) 266, 467, 583
ApaLI GTGCAC 1 cut(s) 287
ApeKI GCWGC 3 cut(s) 425, 428, 611
AspLEI GCGC 4 cut(s) 308, 459, 461, 629
AspS9I GGNCC 2 cut(s) 467, 584
AsuC2I CCSGG 1 cut(s) 670
AsuHPI GGTGA 3 cut(s) 304, 529, 538
BaeGI GKGCMC 1 cut(s) 291
BamHI GGATCC 1 cut(s) 602
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 2 cut(s) 386, 408
BbrPI CACGTG 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 291
BbvCI CCTCAGC 2 cut(s) 356, 549
BbvI GCAGC 3 cut(s) 412, 415, 598
BccI CCATC 3 cut(s) 19, 251, 478
BceAI ACGGC 1 cut(s) 528
BciT130I CCWGG 1 cut(s) 588
BcnI CCSGG 1 cut(s) 670
BcoDI GTCTC 2 cut(s) 304, 730
BfaI CTAG 2 cut(s) 647, 683
BfoI RGCGCY 1 cut(s) 309
BisI GCNGC 3 cut(s) 426, 429, 612
BlsI GCNGC 3 cut(s) 427, 430, 613
Bme1390I CCNGG 2 cut(s) 588, 670
BmgT120I GGNCC 2 cut(s) 467, 584
BmiI GGNNCC 2 cut(s) 307, 604
BmrFI CCNGG 2 cut(s) 588, 670
BmsI GCATC 1 cut(s) 284
Bpu10I CCTNAGC 2 cut(s) 356, 549
BpuEI CTTGAG 1 cut(s) 712
BpuMI CCSGG 1 cut(s) 670
BsaAI YACGTR 1 cut(s) 339
BsaHI GRCGYC 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 586
BsaWI WCCGGW 1 cut(s) 59
Bsc4I CCNNNNNNNGG 2 cut(s) 339, 675
Bse1I ACTGG 2 cut(s) 606, 696
Bse3DI GCAATG 2 cut(s) 69, 420
BseAI TCCGGA 1 cut(s) 59
BseBI CCWGG 1 cut(s) 588
BseDI CCNNGG 1 cut(s) 586
BseGI GGATG 2 cut(s) 11, 275
BseLI CCNNNNNNNGG 2 cut(s) 339, 675
BseMI GCAATG 2 cut(s) 69, 420
BseMII CTCAG 3 cut(s) 370, 540, 552
BseNI ACTGG 2 cut(s) 606, 696
BsePI GCGCGC 1 cut(s) 457
BseRI GAGGAG 1 cut(s) 141
BseSI GKGCMC 1 cut(s) 291
BseXI GCAGC 3 cut(s) 412, 415, 598
Bsh1236I CGCG 1 cut(s) 459
BshFI GGCC 3 cut(s) 268, 469, 585
BshNI GGYRCC 1 cut(s) 305
BsiHKAI GWGCWC 1 cut(s) 291
BsiSI CCGG 3 cut(s) 60, 303, 670
BslI CCNNNNNNNGG 2 cut(s) 339, 675
BsmAI GTCTC 2 cut(s) 304, 730
BsnI GGCC 3 cut(s) 268, 469, 585
Bsp1286I GDGCHC 3 cut(s) 291, 386, 408
Bsp13I TCCGGA 1 cut(s) 59
Bsp143I GATC 4 cut(s) 318, 323, 369, 602
BspACI CCGC 3 cut(s) 178, 333, 515
BspANI GGCC 3 cut(s) 268, 469, 585
BspCNI CTCAG 3 cut(s) 369, 541, 553
BspEI TCCGGA 1 cut(s) 59
BspFNI CGCG 1 cut(s) 459
BspLI GGNNCC 2 cut(s) 307, 604
BspPI GGATC 4 cut(s) 313, 318, 597, 610
BspT107I GGYRCC 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 180
BsrDI GCAATG 2 cut(s) 69, 420
BsrI ACTGG 2 cut(s) 606, 696
BssECI CCNNGG 1 cut(s) 586
BssHII GCGCGC 1 cut(s) 457
BssMI GATC 4 cut(s) 318, 323, 369, 602
BssNI GRCGYC 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 588
Bst4CI ACNGT 3 cut(s) 286, 293, 724
Bst6I CTCTTC 1 cut(s) 560
BstACI GRCGYC 1 cut(s) 306
BstBAI YACGTR 1 cut(s) 339
BstC8I GCNNGC 2 cut(s) 333, 459
BstDEI CTNAG 4 cut(s) 356, 549, 561, 708
BstF5I GGATG 2 cut(s) 11, 275
BstFNI CGCG 1 cut(s) 459
BstH2I RGCGCY 1 cut(s) 309
BstHHI GCGC 4 cut(s) 308, 459, 461, 629
BstKTI GATC 4 cut(s) 321, 326, 372, 605
BstMAI GTCTC 2 cut(s) 304, 730
BstMBI GATC 4 cut(s) 318, 323, 369, 602
BstNI CCWGG 1 cut(s) 588
BstSCI CCNGG 2 cut(s) 586, 668
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 1 cut(s) 459
BstV1I GCAGC 3 cut(s) 412, 415, 598
BstX2I RGATCY 1 cut(s) 602
BstYI RGATCY 1 cut(s) 602
BsuRI GGCC 3 cut(s) 268, 469, 585
BtsCI GGATG 2 cut(s) 11, 275
BtsI GCAGTG 1 cut(s) 598
BtsIMutI CAGTG 5 cut(s) 282, 298, 598, 613, 720
Cac8I GCNNGC 2 cut(s) 333, 459
CaiI CAGNNNCTG 1 cut(s) 702
CfoI GCGC 4 cut(s) 308, 459, 461, 629
Cfr13I GGNCC 2 cut(s) 467, 584
Csp6I GTAC 1 cut(s) 705
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviAII CATG 1 cut(s) 272
CviJI RGCY 9 cut(s) 86, 268, 384, 406, 469, 548, 560, 585, 673
CviKI_1 RGCY 9 cut(s) 86, 268, 384, 406, 469, 548, 560, 585, 673
CviQI GTAC 1 cut(s) 705
DdeI CTNAG 4 cut(s) 356, 549, 561, 708
DinI GGCGCC 1 cut(s) 307
DpnI GATC 4 cut(s) 320, 325, 371, 604
DpnII GATC 4 cut(s) 318, 323, 369, 602
Eam1104I CTCTTC 1 cut(s) 560
EarI CTCTTC 1 cut(s) 560
Eco147I AGGCCT 1 cut(s) 268
Eco24I GRGCYC 2 cut(s) 386, 408
Eco72I CACGTG 1 cut(s) 339
EcoRII CCWGG 1 cut(s) 586
EcoT22I ATGCAT 1 cut(s) 277
EcoT38I GRGCYC 2 cut(s) 386, 408
EgeI GGCGCC 1 cut(s) 307
EheI GGCGCC 1 cut(s) 307
FaeI CATG 1 cut(s) 275
FaiI YATR 5 cut(s) 260, 273, 664, 748, 750
FatI CATG 1 cut(s) 271
FauI CCCGC 1 cut(s) 340
FauNDI CATATG 1 cut(s) 748
Fnu4HI GCNGC 3 cut(s) 426, 429, 612
FokI GGATG 1 cut(s) 262
FriOI GRGCYC 2 cut(s) 386, 408
Fsp4HI GCNGC 3 cut(s) 426, 429, 612
FspBI CTAG 2 cut(s) 647, 683
GlaI GCGC 4 cut(s) 307, 458, 460, 628
GluI GCNGC 3 cut(s) 426, 429, 612
HaeII RGCGCY 1 cut(s) 309
HaeIII GGCC 3 cut(s) 268, 469, 585
HapII CCGG 3 cut(s) 60, 303, 670
HhaI GCGC 4 cut(s) 308, 459, 461, 629
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 1 cut(s) 275
Hin6I GCGC 4 cut(s) 306, 457, 459, 627
HinP1I GCGC 4 cut(s) 306, 457, 459, 627
HincII GTYRAC 1 cut(s) 126
HindII GTYRAC 1 cut(s) 126
HinfI GANTC 1 cut(s) 56
HpaII CCGG 3 cut(s) 60, 303, 670
HphI GGTGA 3 cut(s) 304, 529, 538
Hpy166II GTNNAC 2 cut(s) 126, 289
Hpy188I TCNGA 3 cut(s) 323, 390, 443
Hpy188III TCNNGA 4 cut(s) 53, 60, 367, 637
Hpy8I GTNNAC 2 cut(s) 126, 289
HpyAV CCTTC 4 cut(s) 171, 193, 319, 570
HpyCH4III ACNGT 3 cut(s) 286, 293, 724
HpyCH4IV ACGT 2 cut(s) 338, 761
HpyCH4V TGCA 6 cut(s) 74, 136, 262, 275, 289, 621
HpyF3I CTNAG 4 cut(s) 356, 549, 561, 708
HpySE526I ACGT 2 cut(s) 338, 761
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 1 cut(s) 275
HspAI GCGC 4 cut(s) 306, 457, 459, 627
KasI GGCGCC 1 cut(s) 305
Kpn2I TCCGGA 1 cut(s) 59
Kzo9I GATC 4 cut(s) 318, 323, 369, 602
LmnI GCTCC 1 cut(s) 185
Lsp1109I GCAGC 3 cut(s) 412, 415, 598
LweI GCATC 1 cut(s) 284
MaeI CTAG 2 cut(s) 647, 683
MaeII ACGT 2 cut(s) 338, 761
MaeIII GTNAC 2 cut(s) 517, 716
MalI GATC 4 cut(s) 320, 325, 371, 604
MbiI CCGCTC 1 cut(s) 180
MboI GATC 4 cut(s) 318, 323, 369, 602
MboII GAAGA 3 cut(s) 178, 364, 577
MflI RGATCY 1 cut(s) 602
MhlI GDGCHC 3 cut(s) 291, 386, 408
MluCI AATT 2 cut(s) 42, 192
Mly113I GGCGCC 1 cut(s) 306
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 52
Mph1103I ATGCAT 1 cut(s) 277
MroI TCCGGA 1 cut(s) 59
MslI CAYNNNNRTG 2 cut(s) 284, 515
MspI CCGG 3 cut(s) 60, 303, 670
MspR9I CCNGG 2 cut(s) 588, 670
MvaI CCWGG 1 cut(s) 588
MvnI CGCG 1 cut(s) 459
NarI GGCGCC 1 cut(s) 306
NciI CCSGG 1 cut(s) 670
NdeI CATATG 1 cut(s) 748
NdeII GATC 4 cut(s) 318, 323, 369, 602
NlaIII CATG 1 cut(s) 275
NlaIV GGNNCC 2 cut(s) 307, 604
NmuCI GTSAC 2 cut(s) 517, 716
NsiI ATGCAT 1 cut(s) 277
OliI CACNNNNGTG 2 cut(s) 284, 515
PauI GCGCGC 1 cut(s) 457
PceI AGGCCT 1 cut(s) 268
PcsI WCGNNNNNNNCGW 1 cut(s) 335
PflFI GACNNNGTC 1 cut(s) 722
PkrI GCNGC 3 cut(s) 427, 430, 613
PleI GAGTC 1 cut(s) 64
PluTI GGCGCC 1 cut(s) 309
PmaCI CACGTG 1 cut(s) 339
PmlI CACGTG 1 cut(s) 339
PpsI GAGTC 1 cut(s) 64
Ppu21I YACGTR 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 586
PspCI CACGTG 1 cut(s) 339
PspGI CCWGG 1 cut(s) 586
PspN4I GGNNCC 2 cut(s) 307, 604
PspPI GGNCC 2 cut(s) 467, 584
PstNI CAGNNNCTG 1 cut(s) 702
PsuI RGATCY 1 cut(s) 602
PsyI GACNNNGTC 1 cut(s) 722
PteI GCGCGC 1 cut(s) 457
RsaI GTAC 1 cut(s) 706
RsaNI GTAC 1 cut(s) 705
RseI CAYNNNNRTG 2 cut(s) 284, 515
SatI GCNGC 3 cut(s) 426, 429, 612
Sau3AI GATC 4 cut(s) 318, 323, 369, 602
Sau96I GGNCC 2 cut(s) 467, 584
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 2 cut(s) 588, 670
SduI GDGCHC 3 cut(s) 291, 386, 408
SfaNI GCATC 1 cut(s) 284
SfoI GGCGCC 1 cut(s) 307
SmiMI CAYNNNNRTG 2 cut(s) 284, 515
SmlI CTYRAG 1 cut(s) 727
SmoI CTYRAG 1 cut(s) 727
Sse9I AATT 2 cut(s) 42, 192
SseBI AGGCCT 1 cut(s) 268
SsiI CCGC 3 cut(s) 178, 333, 515
SspDI GGCGCC 1 cut(s) 305
SspMI CTAG 2 cut(s) 647, 683
StuI AGGCCT 1 cut(s) 268
StyD4I CCNGG 2 cut(s) 586, 668
TaaI ACNGT 3 cut(s) 286, 293, 724
TaiI ACGT 2 cut(s) 341, 764
TasI AATT 2 cut(s) 42, 192
TatI WGTACW 1 cut(s) 704
TscAI CASTG 5 cut(s) 289, 298, 598, 613, 727
TseFI GTSAC 2 cut(s) 517, 716
TseI GCWGC 3 cut(s) 425, 428, 611
Tsp45I GTSAC 2 cut(s) 517, 716
TspDTI ATGAA 1 cut(s) 490
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 5 cut(s) 289, 298, 598, 613, 727
Tth111I GACNNNGTC 1 cut(s) 722
VneI GTGCAC 1 cut(s) 287
XspI CTAG 2 cut(s) 647, 683
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.