Rw5G018840

Integral membrane protein DUF92

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008669
Physical Location & Seq
Reverse (-)
24277252 .. 24281156
3905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G018840.1

Sequence Viewer

Length: 966 bp
ATGGCATTGTCAACACTCTTGCACATCAAACCACCTCCTCCGTTTCCTTCACTAAAATCCCACCGCTCCTTCTTCCCAATTCTCCCCGCAAAAATCTCAATCCCAATCCAAAACCCTAAACCCATCAAACACTTCACTATGCAGAGGCCTCAAGCATCCACCACTGTGCACAGTGCTGTCTCCGGCGCCTTCACCCTGATCCGATCCTCGCCCGCCACGTGGCAGTCGGCAATCCTCAGCAACCTCCTGATCTTCGTTCTGGGCTCTCCGATTTTGGTTTCTGGGCTCTCCCTTTCCGGCATTGCTGCTGCGTTTTTGCTCGGAACTCTCACTTGGCGCGCTTTTGGGCCATCTGGGTTTCTCTTGGTGGCAACCTACTTCATCATTGGCACGGCGGTGACAAAGGTGAGAATGGCACAAAAGGAGGCTGAGGGGGTAGCCGAGAAGAGAAAAGGAAGGAGAGGCCCAGGCAGTGTTATTGGATCCAGTGCTGCTGGTTGCATTTGTGCTTTTCTCACGATATTTCTAGTAGGAGGAAAGGCATATTCCCGGCTCTGGCAACTAGGGTTTGTTGCCAGTTTCTGTACTAAGTTGAGTGATACTGTCTCAAGTGAGGTGGGAAAGGCATATGGGAAAACAACGTACTTGGTAACAACATTGAAGGTAGTTCCACGGGGAACAGAGGGAGCTGTGAGTCTTGAGGGAACCATTGCTGGACTTTTGGCATCTGTTCTTCTTGCGTTTGTTGGTTGTCTCATGGGTCAGATAAATGGACCTGAAGCAATTATATGTGTAATAGCTTCCCAAATAGCAAATGTTGGTGAGAGCATTATAGGTGCTGCTCTTCAAGACAAGGAAGGATTTCGATGGCTCAACAATGATGCTGTGAATGTCATAAACATATCCATGGGCAGCATTTTTGCCGTCTTGATGCAGCAGGCATTACTCAAAAACTTACATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF92

Protein Analysis

321

Amino Acids

33.87

Weight (kDa)

9.95

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF92 PF01940 85 - 304 8.1e-59 Integral membrane protein DUF92
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 185
AccBSI CCGCTC 1 cut(s) 66
AccII CGCG 1 cut(s) 339
AciI CCGC 4 cut(s) 64, 87, 213, 395
AclWI GGATC 4 cut(s) 193, 198, 477, 490
AcuI CTGAAG 1 cut(s) 798
AcvI CACGTG 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 2 cut(s) 586, 644
AfiI CCNNNNNNNGG 2 cut(s) 219, 555
AgsI TTSAA 2 cut(s) 661, 848
AjnI CCWGG 1 cut(s) 466
AjuI GAANNNNNNNTTGG 2 cut(s) 316, 348
AleI CACNNNNGTG 2 cut(s) 164, 395
AluBI AGCT 2 cut(s) 689, 800
AluI AGCT 2 cut(s) 689, 800
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 3 cut(s) 184, 610, 758
Alw44I GTGCAC 1 cut(s) 167
AlwI GGATC 4 cut(s) 193, 198, 477, 490
AlwNI CAGNNNCTG 1 cut(s) 582
AoxI GGCC 3 cut(s) 146, 347, 463
ApaLI GTGCAC 1 cut(s) 167
ApeKI GCWGC 6 cut(s) 305, 308, 491, 839, 912, 934
Asp700I GAANNNNTTC 1 cut(s) 861
AspLEI GCGC 3 cut(s) 188, 339, 341
AspS9I GGNCC 3 cut(s) 347, 464, 773
AsuC2I CCSGG 1 cut(s) 550
AsuHPI GGTGA 4 cut(s) 184, 409, 418, 833
AvaII GGWCC 1 cut(s) 773
BaeGI GKGCMC 1 cut(s) 171
BamHI GGATCC 1 cut(s) 482
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 2 cut(s) 266, 288
BbrPI CACGTG 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 171
BbvCI CCTCAGC 2 cut(s) 236, 429
BbvI GCAGC 6 cut(s) 292, 295, 478, 826, 924, 946
BccI CCATC 3 cut(s) 131, 358, 861
BceAI ACGGC 2 cut(s) 408, 908
BciT130I CCWGG 1 cut(s) 468
BcnI CCSGG 1 cut(s) 550
BcoDI GTCTC 3 cut(s) 184, 610, 758
BfaI CTAG 2 cut(s) 527, 563
BfoI RGCGCY 1 cut(s) 189
BisI GCNGC 6 cut(s) 306, 309, 492, 840, 913, 935
BlsI GCNGC 6 cut(s) 307, 310, 493, 841, 914, 936
Bme1390I CCNGG 2 cut(s) 468, 550
Bme18I GGWCC 1 cut(s) 773
BmgT120I GGNCC 3 cut(s) 347, 464, 773
BmiI GGNNCC 3 cut(s) 187, 484, 706
BmrFI CCNGG 2 cut(s) 468, 550
BmsI GCATC 4 cut(s) 164, 734, 871, 921
Bpu10I CCTNAGC 2 cut(s) 236, 429
BpuEI CTTGAG 3 cut(s) 135, 592, 719
BpuMI CCSGG 1 cut(s) 550
BsaAI YACGTR 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 3 cut(s) 466, 671, 906
Bsc4I CCNNNNNNNGG 2 cut(s) 219, 555
Bse1I ACTGG 2 cut(s) 486, 576
Bse3DI GCAATG 2 cut(s) 300, 708
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 3 cut(s) 466, 671, 906
BseGI GGATG 1 cut(s) 155
BseLI CCNNNNNNNGG 2 cut(s) 219, 555
BseMI GCAATG 2 cut(s) 300, 708
BseMII CTCAG 2 cut(s) 250, 420
BseNI ACTGG 2 cut(s) 486, 576
BsePI GCGCGC 1 cut(s) 337
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 6 cut(s) 292, 295, 478, 826, 924, 946
Bsh1236I CGCG 1 cut(s) 339
BshFI GGCC 3 cut(s) 148, 349, 465
BshNI GGYRCC 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 3 cut(s) 183, 297, 550
BslI CCNNNNNNNGG 2 cut(s) 219, 555
BsmAI GTCTC 3 cut(s) 184, 610, 758
BsnI GGCC 3 cut(s) 148, 349, 465
Bsp1286I GDGCHC 3 cut(s) 171, 266, 288
Bsp143I GATC 4 cut(s) 198, 203, 249, 482
Bsp19I CCATGG 1 cut(s) 906
BspACI CCGC 4 cut(s) 64, 87, 213, 395
BspANI GGCC 3 cut(s) 148, 349, 465
BspCNI CTCAG 2 cut(s) 249, 421
BspFNI CGCG 1 cut(s) 339
BspLI GGNNCC 3 cut(s) 187, 484, 706
BspPI GGATC 4 cut(s) 193, 198, 477, 490
BspQI GCTCTTC 1 cut(s) 849
BspT107I GGYRCC 1 cut(s) 185
BsrBI CCGCTC 1 cut(s) 66
BsrDI GCAATG 2 cut(s) 300, 708
BsrI ACTGG 2 cut(s) 486, 576
BssECI CCNNGG 3 cut(s) 466, 671, 906
BssHII GCGCGC 1 cut(s) 337
BssMI GATC 4 cut(s) 198, 203, 249, 482
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 906
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 3 cut(s) 166, 173, 604
Bst6I CTCTTC 2 cut(s) 440, 849
BstACI GRCGYC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 219
BstC8I GCNNGC 3 cut(s) 213, 339, 939
BstDEI CTNAG 3 cut(s) 236, 429, 588
BstDSI CCRYGG 2 cut(s) 671, 906
BstF5I GGATG 1 cut(s) 155
BstFNI CGCG 1 cut(s) 339
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 3 cut(s) 188, 339, 341
BstKTI GATC 4 cut(s) 201, 206, 252, 485
BstMAI GTCTC 3 cut(s) 184, 610, 758
BstMBI GATC 4 cut(s) 198, 203, 249, 482
BstNI CCWGG 1 cut(s) 468
BstSCI CCNGG 2 cut(s) 466, 548
BstSLI GKGCMC 1 cut(s) 171
BstUI CGCG 1 cut(s) 339
BstV1I GCAGC 6 cut(s) 292, 295, 478, 826, 924, 946
BstX2I RGATCY 1 cut(s) 482
BstYI RGATCY 1 cut(s) 482
BsuRI GGCC 3 cut(s) 148, 349, 465
BtgI CCRYGG 2 cut(s) 671, 906
BtsCI GGATG 1 cut(s) 155
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 4 cut(s) 162, 178, 478, 493
Cac8I GCNNGC 3 cut(s) 213, 339, 939
CaiI CAGNNNCTG 1 cut(s) 582
CfoI GCGC 3 cut(s) 188, 339, 341
Cfr13I GGNCC 3 cut(s) 347, 464, 773
Csp6I GTAC 2 cut(s) 585, 643
CspCI CAANNNNNGTGG 2 cut(s) 597, 632
CviAII CATG 2 cut(s) 757, 907
CviQI GTAC 2 cut(s) 585, 643
DdeI CTNAG 3 cut(s) 236, 429, 588
DinI GGCGCC 1 cut(s) 187
DpnI GATC 4 cut(s) 200, 205, 251, 484
DpnII GATC 4 cut(s) 198, 203, 249, 482
Eam1104I CTCTTC 2 cut(s) 440, 849
EarI CTCTTC 2 cut(s) 440, 849
Eco130I CCWWGG 1 cut(s) 906
Eco147I AGGCCT 1 cut(s) 148
Eco24I GRGCYC 2 cut(s) 266, 288
Eco47I GGWCC 1 cut(s) 773
Eco57I CTGAAG 1 cut(s) 798
Eco72I CACGTG 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 906
EcoT38I GRGCYC 2 cut(s) 266, 288
EgeI GGCGCC 1 cut(s) 187
EheI GGCGCC 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 906
FaeI CATG 2 cut(s) 760, 910
FatI CATG 2 cut(s) 756, 906
FauI CCCGC 2 cut(s) 94, 220
FauNDI CATATG 2 cut(s) 628, 960
Fnu4HI GCNGC 6 cut(s) 306, 309, 492, 840, 913, 935
FokI GGATG 1 cut(s) 142
FriOI GRGCYC 2 cut(s) 266, 288
Fsp4HI GCNGC 6 cut(s) 306, 309, 492, 840, 913, 935
FspBI CTAG 2 cut(s) 527, 563
GlaI GCGC 3 cut(s) 187, 338, 340
GluI GCNGC 6 cut(s) 306, 309, 492, 840, 913, 935
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 3 cut(s) 148, 349, 465
HapII CCGG 3 cut(s) 183, 297, 550
HhaI GCGC 3 cut(s) 188, 339, 341
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 2 cut(s) 760, 910
Hin6I GCGC 3 cut(s) 186, 337, 339
HinP1I GCGC 3 cut(s) 186, 337, 339
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HinfI GANTC 1 cut(s) 694
HpaII CCGG 3 cut(s) 183, 297, 550
HphI GGTGA 4 cut(s) 184, 409, 418, 833
Hpy166II GTNNAC 2 cut(s) 12, 169
Hpy188I TCNGA 4 cut(s) 203, 270, 323, 765
Hpy188III TCNNGA 5 cut(s) 247, 517, 698, 848, 928
Hpy8I GTNNAC 2 cut(s) 12, 169
HpyAV CCTTC 6 cut(s) 57, 79, 199, 450, 655, 851
HpyCH4III ACNGT 3 cut(s) 166, 173, 604
HpyCH4IV ACGT 2 cut(s) 218, 641
HpyCH4V TGCA 5 cut(s) 22, 142, 169, 501, 934
HpyF3I CTNAG 3 cut(s) 236, 429, 588
HpySE526I ACGT 2 cut(s) 218, 641
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 2 cut(s) 760, 910
HspAI GCGC 3 cut(s) 186, 337, 339
KasI GGCGCC 1 cut(s) 185
Kzo9I GATC 4 cut(s) 198, 203, 249, 482
LguI GCTCTTC 1 cut(s) 849
LmnI GCTCC 2 cut(s) 71, 686
Lsp1109I GCAGC 6 cut(s) 292, 295, 478, 826, 924, 946
LweI GCATC 4 cut(s) 164, 734, 871, 921
MaeI CTAG 2 cut(s) 527, 563
MaeII ACGT 2 cut(s) 218, 641
MaeIII GTNAC 2 cut(s) 397, 649
MalI GATC 4 cut(s) 200, 205, 251, 484
MbiI CCGCTC 1 cut(s) 66
MboI GATC 4 cut(s) 198, 203, 249, 482
MboII GAAGA 5 cut(s) 64, 244, 457, 725, 836
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 3 cut(s) 171, 266, 288
MluCI AATT 2 cut(s) 78, 783
Mly113I GGCGCC 1 cut(s) 186
MlyI GAGTC 1 cut(s) 703
MroXI GAANNNNTTC 1 cut(s) 861
MslI CAYNNNNRTG 3 cut(s) 164, 395, 905
MspI CCGG 3 cut(s) 183, 297, 550
MspR9I CCNGG 2 cut(s) 468, 550
MvaI CCWGG 1 cut(s) 468
MvnI CGCG 1 cut(s) 339
NarI GGCGCC 1 cut(s) 186
NciI CCSGG 1 cut(s) 550
NcoI CCATGG 1 cut(s) 906
NdeI CATATG 2 cut(s) 628, 960
NdeII GATC 4 cut(s) 198, 203, 249, 482
NlaIII CATG 2 cut(s) 760, 910
NlaIV GGNNCC 3 cut(s) 187, 484, 706
NmeAIII GCCGAG 1 cut(s) 466
NmuCI GTSAC 1 cut(s) 397
OliI CACNNNNGTG 2 cut(s) 164, 395
PauI GCGCGC 1 cut(s) 337
PceI AGGCCT 1 cut(s) 148
PciSI GCTCTTC 1 cut(s) 849
PcsI WCGNNNNNNNCGW 1 cut(s) 215
PdmI GAANNNNTTC 1 cut(s) 861
PkrI GCNGC 6 cut(s) 307, 310, 493, 841, 914, 936
PleI GAGTC 1 cut(s) 702
PluTI GGCGCC 1 cut(s) 189
PmaCI CACGTG 1 cut(s) 219
PmlI CACGTG 1 cut(s) 219
PpsI GAGTC 1 cut(s) 702
Ppu21I YACGTR 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 466
PspCI CACGTG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 3 cut(s) 187, 484, 706
PspPI GGNCC 3 cut(s) 347, 464, 773
PstNI CAGNNNCTG 1 cut(s) 582
PsuI RGATCY 1 cut(s) 482
PteI GCGCGC 1 cut(s) 337
RsaI GTAC 2 cut(s) 586, 644
RsaNI GTAC 2 cut(s) 585, 643
RseI CAYNNNNRTG 3 cut(s) 164, 395, 905
SapI GCTCTTC 1 cut(s) 849
SatI GCNGC 6 cut(s) 306, 309, 492, 840, 913, 935
Sau3AI GATC 4 cut(s) 198, 203, 249, 482
Sau96I GGNCC 3 cut(s) 347, 464, 773
SchI GAGTC 1 cut(s) 703
ScrFI CCNGG 2 cut(s) 468, 550
SduI GDGCHC 3 cut(s) 171, 266, 288
SfaNI GCATC 4 cut(s) 164, 734, 871, 921
SfoI GGCGCC 1 cut(s) 187
SinI GGWCC 1 cut(s) 773
SmiMI CAYNNNNRTG 3 cut(s) 164, 395, 905
SmlI CTYRAG 3 cut(s) 150, 607, 698
SmoI CTYRAG 3 cut(s) 150, 607, 698
Sse9I AATT 2 cut(s) 78, 783
SseBI AGGCCT 1 cut(s) 148
SsiI CCGC 4 cut(s) 64, 87, 213, 395
SspDI GGCGCC 1 cut(s) 185
SspMI CTAG 2 cut(s) 527, 563
StuI AGGCCT 1 cut(s) 148
StyD4I CCNGG 2 cut(s) 466, 548
StyI CCWWGG 1 cut(s) 906
TaaI ACNGT 3 cut(s) 166, 173, 604
TaiI ACGT 2 cut(s) 221, 644
TaqI TCGA 1 cut(s) 865
TasI AATT 2 cut(s) 78, 783
TatI WGTACW 1 cut(s) 584
TscAI CASTG 4 cut(s) 169, 178, 478, 493
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 6 cut(s) 305, 308, 491, 839, 912, 934
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 1 cut(s) 370
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 4 cut(s) 169, 178, 478, 493
VneI GTGCAC 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 773
XmnI GAANNNNTTC 1 cut(s) 861
XspI CTAG 2 cut(s) 527, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.