Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002563

Physical Location & Seq

Genomic Coordinates Reverse (-)

33919970 .. 33925118

Gene Structure 5149 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1251600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1827 bp

ATGATGCGTCCTGATCACCAAAATAAGGAGCTGAAAGAGATGGACTTTTTCACCGAATATGGTGAAGCTAATAGATATAAGATTCTCGAAGTCATAGGGAAGGGAAGTTATGGGGTTGTTTGTGCAGCTATTGACACACATACTGGGGAGAAAGTTGCAATAAAGAAAATACATGATATCTTTGAGCACATCTCTGATGCCTTTAGGATCTTGCGTGAAGTTAAGTTGCTTAGACTTTTAAGACATCCTGATATTGTTGAAATTAAGCGGATCATGTTGCCACCTTCAAAGAAAGAGTTCAAAGATATTTATGTTGTTTTTGAGCTCATGGAGTCAGATCTTCACCAAGTTATCAAGGCTAATGATGACTTGACCCGCGAACACCTTCAATTCTTTCTTTACCAGATGTTACGTGCTTTGAAATATATGCATACAGCAAATGTGTATCATCGAGATCTTAAGCCAAAAAATATACTGGCAAATGCAAATTGCAAGCTCAAAGTTTGTGATTTTGGACTAGCAAGAGTTGCCTTTAATGATACTCCAACAACAATATTTTGGACAGACTATGTTGCTACAAGATGGTATAGAGCTCCAGAATTATGTGGGTCATTCCTTTCCAAGTACACACCTGCAATTGATATATGGAGTATTGGCTGCATCTTTGCCGAGGTATTGACAGGGAAGCCATTGTTTCCGGGTAAAAGCGTTATTCATCAATTAGATTTGATCACTGATCTCCTTGGAACACCTCCGCTCGAGACCATCTCTGGAGTTAGAAATGAGAAGGCAAGGAAATACTTGGAAATGCAGAGAAAACCTCCTGTGCCATTTACACAAAAATTTCCTAAAGCAGATCCTCTAGCACTGCGCCTATTACAAAGGCTATTGGCTTTTGACCCAAAGGATAGACCAACTGCTGAGCAGGCACTAGCTGATCCTTACTTCAAGGGTCTAGCCAAAGTTGAGAGAGAACCTTCTTGTCAGCCTATTTCGAAGATGGAATTTGAATTTGAAAGGCGTAGAGTCACAAAGGAGGACATCAGGGAACTAATTTACCGGGAAATACTAGAATACCATCCTCAGCTACTTAAGGACTACATGAATGGGACTGAAGGCACAAGTTTTATGTATCCGAGTGCAATAGGCCAATTCAGAAAGCAGTTTGCTTATCTTGAGGAAAATGGTGGGAAAAGTGGACCGGTTATTCCTCCAGAGAGGAAGCATGTCTCCCTTCCACGATCCACCGTTCACTCAAGTACAGTCCCCCCTAATGCACAGTCAAATTTGGTCTCGTATGAGAACCGACAGACACAAGAGGCTTCTAGCAGTTTTAGAGTAACGGACACAATTTCTGGAAATGCACCAAAAGTTTCAAGGCCACCACCGAGGGTGCCAACAGCAAAACCTGGGCGAGTTGTTGGACCAATTCTTCCATACGAGAATGGCAGAAATGTGAAGGAAATGTATGATCCGAGGATGTTATACAGAAACGCAGTTCCTCCTCAGTCTGTCTCTCCACACTGCTTCTTCAGAATTCATACTGCCAATCAAGACAAGGCTGGAGTGGAGGTGCAGAGGTATGAATCACATGCTAAACTGCAACCCCAACCCGAACAGTGCAACTTAGCAGCAAAGTCTTCTCCGGGCATGGCCATTGAAGTCAATACGAACCCATACTACCCGCCACCCGCCAAAGTAGATCAATTAAACTCGATAGATTCAAAATTACTGCACGCGCAATCTCAGTTTGGTCCTGTAGGTGCCGCAGCTGTAGCTGTAGCTGCTCACCGGAACACTGGAGCTGTTCAGTATGGATTGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000165 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

609

Amino Acids

69.08

Weight (kDa)

9.14

Isoelectric Point (pI)

38.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	26 - 316	6.4e-64	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	27 - 309	2.6e-31	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002563)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53510 AT3G14720 AT3G14720 AT3G14720
fragaria_vesca	FvH4_3g17440 FvH4_3g17440 FvH4_3g17440 FvH4_6g38240 FvH4_6g38240 FvH4_6g38240 FvH4_6g38240 FvH4_6g38240
malus_domestica	MD03G1251600.v1.1 MD09G1151800.v1.1 MD11G1272700.v1.1 MD17G1139400.v1.1
prunus_persica	Prupe.3G172800_v2.0.a1 Prupe.3G172800_v2.0.a1 Prupe.4G154700_v2.0.a1
pyrus_communis	pycom03g19900
rosa_chinensis	RchiOBHm_Chr2g0151501 RchiOBHm_Chr5g0028971
rosa_laevigata	RLG00000020555 RLG00000033132
rosa_multiflora	Rmu_sc0000940.1_g000020 Rmu_sc0010209.1_g000001
rosa_roxburghii	Rroxscaffold_1G00050920 Rroxscaffold_2G00096400
rosa_rugosa	Rorug02G0426600 Rorug05G0112100
rosa_samantha	Rh2AG487400 Rh2BG499800 Rh2CG473800 Rh2DG511000 Rh5AG204100 Rh5BG202400 Rh5CG223200 Rh5DG206200
rosa_wichuraiana	Rw2G039950 Rw5G018500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	640
Acc36I	ACCTGC	1 cut(s)	640
AccB1I	GGYRCC	2 cut(s)	1393, 1763
AccBSI	CCGCTC	1 cut(s)	757
AccII	CGCG	2 cut(s)	378, 1739
AciI	CCGC	6 cut(s)	268, 376, 755, 1685, 1692, 1767
AclWI	GGATC	6 cut(s)	215, 278, 851, 932, 1236, 1466
AcoI	YGGCCR	1 cut(s)	1653
AcsI	RAATTY	5 cut(s)	842, 1004, 1010, 1285, 1536
AcuI	CTGAAG	2 cut(s)	1134, 1516
AfaI	GTAC	2 cut(s)	626, 1261
AfiI	CCNNNNNNNGG	1 cut(s)	25
AflII	CTTAAG	2 cut(s)	458, 1091
AgeI	ACCGGT	1 cut(s)	1201
AjnI	CCWGG	1 cut(s)	1408
Alw21I	GWGCWC	3 cut(s)	189, 327, 595
Alw26I	GTCTC	4 cut(s)	755, 1234, 1297, 1519
AlwI	GGATC	6 cut(s)	215, 278, 851, 932, 1236, 1466
Ama87I	CYCGRG	1 cut(s)	758
AoxI	GGCC	3 cut(s)	1147, 1379, 1653
ApeKI	GCWGC	5 cut(s)	125, 657, 1631, 1769, 1784
ApoI	RAATTY	5 cut(s)	842, 1004, 1010, 1285, 1536
AsiGI	ACCGGT	1 cut(s)	1201
Asp700I	GAANNNNTTC	2 cut(s)	296, 384
AspLEI	GCGC	2 cut(s)	873, 1741
AspS9I	GGNCC	3 cut(s)	1199, 1424, 1754
AsuC2I	CCSGG	3 cut(s)	699, 1061, 1647
AsuHPI	GGTGA	5 cut(s)	8, 43, 74, 335, 1781
AsuII	TTCGAA	1 cut(s)	995
AvaI	CYCGRG	1 cut(s)	758
AvaII	GGWCC	3 cut(s)	1199, 1424, 1754
BalI	TGGCCA	1 cut(s)	1655
BanI	GGYRCC	2 cut(s)	1393, 1763
BanII	GRGCYC	2 cut(s)	327, 595
BbsI	GAAGAC	1 cut(s)	1632
Bbv12I	GWGCWC	3 cut(s)	189, 327, 595
BbvCI	CCTCAGC	1 cut(s)	1083
BbvI	GCAGC	5 cut(s)	137, 644, 1643, 1771, 1781
BccI	CCATC	5 cut(s)	34, 576, 773, 994, 1086
BciT130I	CCWGG	1 cut(s)	1410
BciVI	GTATCC	1 cut(s)	1143
BclI	TGATCA	2 cut(s)	13, 729
BcnI	CCSGG	3 cut(s)	699, 1061, 1647
BcoDI	GTCTC	4 cut(s)	755, 1234, 1297, 1519
BfaI	CTAG	6 cut(s)	518, 863, 932, 956, 1070, 1326
BfmI	CTRYAG	3 cut(s)	1758, 1773, 1779
BfrI	CTTAAG	2 cut(s)	458, 1091
BfuAI	ACCTGC	1 cut(s)	640
BfuI	GTATCC	1 cut(s)	1143
BglII	AGATCT	2 cut(s)	337, 454
BisI	GCNGC	6 cut(s)	126, 658, 1632, 1767, 1770, 1785
BlpI	GCTNAGC	1 cut(s)	921
BlsI	GCNGC	6 cut(s)	127, 659, 1633, 1768, 1771, 1786
Bme1390I	CCNGG	4 cut(s)	699, 1061, 1410, 1647
Bme18I	GGWCC	3 cut(s)	1199, 1424, 1754
BmeT110I	CYCGRG	1 cut(s)	758
BmgT120I	GGNCC	3 cut(s)	1199, 1424, 1754
BmiI	GGNNCC	2 cut(s)	1395, 1765
BmrFI	CCNGG	4 cut(s)	699, 1061, 1410, 1647
BmrI	ACTGGG	1 cut(s)	153
BmsI	GCATC	2 cut(s)	187, 669
BmuI	ACTGGG	1 cut(s)	153
BpiI	GAAGAC	1 cut(s)	1632
BplI	GAGNNNNNCTC	6 cut(s)	176, 208, 752, 784, 805, 837
BpmI	CTGGAG	5 cut(s)	579, 792, 1197, 1584, 1821
Bpu10I	CCTNAGC	1 cut(s)	1083
Bpu1102I	GCTNAGC	1 cut(s)	921
Bpu14I	TTCGAA	1 cut(s)	995
BpuEI	CTTGAG	2 cut(s)	1196, 1240
BpuMI	CCSGG	3 cut(s)	699, 1061, 1647
BsaAI	YACGTR	1 cut(s)	413
BsaI	GGTCTC	2 cut(s)	755, 1297
BsaJI	CCNNGG	5 cut(s)	669, 742, 1388, 1409, 1475
BsaWI	WCCGGW	2 cut(s)	1201, 1791
BsaXI	ACNNNNNCTCC	2 cut(s)	1557, 1587
Bsc4I	CCNNNNNNNGG	1 cut(s)	25
Bse118I	RCCGGY	1 cut(s)	1201
Bse1I	ACTGG	3 cut(s)	148, 480, 1804
BseBI	CCWGG	1 cut(s)	1410
BseDI	CCNNGG	5 cut(s)	669, 742, 1388, 1409, 1475
BseGI	GGATG	3 cut(s)	244, 1078, 1485
BseLI	CCNNNNNNNGG	1 cut(s)	25
BseMII	CTCAG	4 cut(s)	912, 1097, 1520, 1760
BseNI	ACTGG	3 cut(s)	148, 480, 1804
BseRI	GAGGAG	1 cut(s)	1494
BseXI	GCAGC	5 cut(s)	137, 644, 1643, 1771, 1781
BsgI	GTGCAG	3 cut(s)	144, 1595, 1718
Bsh1236I	CGCG	2 cut(s)	378, 1739
BshFI	GGCC	3 cut(s)	1149, 1381, 1655
BshNI	GGYRCC	2 cut(s)	1393, 1763
BshTI	ACCGGT	1 cut(s)	1201
BsiHKAI	GWGCWC	3 cut(s)	189, 327, 595
BsiHKCI	CYCGRG	1 cut(s)	758
BsiSI	CCGG	5 cut(s)	698, 1060, 1202, 1646, 1792
BslFI	GGGAC	2 cut(s)	1123, 1250
BslI	CCNNNNNNNGG	1 cut(s)	25
BsmAI	GTCTC	4 cut(s)	755, 1234, 1297, 1519
BsmFI	GGGAC	2 cut(s)	1123, 1250
BsnI	GGCC	3 cut(s)	1149, 1381, 1655
Bso31I	GGTCTC	2 cut(s)	755, 1297
BsoBI	CYCGRG	1 cut(s)	758
Bsp119I	TTCGAA	1 cut(s)	995
Bsp1286I	GDGCHC	3 cut(s)	189, 327, 595
Bsp1720I	GCTNAGC	1 cut(s)	921
BspACI	CCGC	6 cut(s)	268, 376, 755, 1685, 1692, 1767
BspANI	GGCC	3 cut(s)	1149, 1381, 1655
BspCNI	CTCAG	4 cut(s)	913, 1096, 1519, 1759
BspFNI	CGCG	2 cut(s)	378, 1739
BspLI	GGNNCC	2 cut(s)	1395, 1765
BspMI	ACCTGC	1 cut(s)	640
BspPI	GGATC	6 cut(s)	215, 278, 851, 932, 1236, 1466
BspT104I	TTCGAA	1 cut(s)	995
BspT107I	GGYRCC	2 cut(s)	1393, 1763
BspTI	CTTAAG	2 cut(s)	458, 1091
BspTNI	GGTCTC	2 cut(s)	755, 1297
BsrBI	CCGCTC	1 cut(s)	757
BsrFI	RCCGGY	1 cut(s)	1201
BsrI	ACTGG	3 cut(s)	148, 480, 1804
BssAI	RCCGGY	1 cut(s)	1201
BssECI	CCNNGG	5 cut(s)	669, 742, 1388, 1409, 1475
BssT1I	CCWWGG	1 cut(s)	742
Bst2UI	CCWGG	1 cut(s)	1410
Bst4CI	ACNGT	4 cut(s)	1249, 1264, 1281, 1620
BstAFI	CTTAAG	2 cut(s)	458, 1091
BstAPI	GCANNNNNTGC	1 cut(s)	527
BstBAI	YACGTR	1 cut(s)	413
BstBI	TTCGAA	1 cut(s)	995
BstC8I	GCNNGC	3 cut(s)	494, 927, 1737
BstDEI	CTNAG	6 cut(s)	230, 921, 1083, 1506, 1627, 1746
BstF5I	GGATG	3 cut(s)	244, 1078, 1485
BstFNI	CGCG	2 cut(s)	378, 1739
BstHHI	GCGC	2 cut(s)	873, 1741
BstMAI	GTCTC	4 cut(s)	755, 1234, 1297, 1519
BstMWI	GCNNNNNNNGC	4 cut(s)	527, 926, 1775, 1784
BstNI	CCWGG	1 cut(s)	1410
BstNSI	RCATGY	2 cut(s)	1229, 1595
BstSCI	CCNGG	4 cut(s)	697, 1059, 1408, 1645
BstSFI	CTRYAG	3 cut(s)	1758, 1773, 1779
BstUI	CGCG	2 cut(s)	378, 1739
BstV1I	GCAGC	5 cut(s)	137, 644, 1643, 1771, 1781
BstV2I	GAAGAC	1 cut(s)	1632
BstX2I	RGATCY	4 cut(s)	207, 337, 454, 856
BstYI	RGATCY	4 cut(s)	207, 337, 454, 856
BsuI	GTATCC	1 cut(s)	1143
BsuRI	GGCC	3 cut(s)	1149, 1381, 1655
BtsCI	GGATG	3 cut(s)	244, 1078, 1485
BtsI	GCAGTG	2 cut(s)	866, 1522
BtsIMutI	CAGTG	5 cut(s)	732, 866, 1522, 1625, 1797
BveI	ACCTGC	1 cut(s)	640
Cac8I	GCNNGC	3 cut(s)	494, 927, 1737
CfoI	GCGC	2 cut(s)	873, 1741
Cfr10I	RCCGGY	1 cut(s)	1201
Cfr13I	GGNCC	3 cut(s)	1199, 1424, 1754
Csp6I	GTAC	2 cut(s)	625, 1260
CspAI	ACCGGT	1 cut(s)	1201
CviAII	CATG	7 cut(s)	173, 274, 328, 1102, 1226, 1592, 1651
CviQI	GTAC	2 cut(s)	625, 1260
DdeI	CTNAG	6 cut(s)	230, 921, 1083, 1506, 1627, 1746
EaeI	YGGCCR	1 cut(s)	1653
Ecl136II	GAGCTC	2 cut(s)	325, 593
Eco130I	CCWWGG	1 cut(s)	742
Eco24I	GRGCYC	2 cut(s)	327, 595
Eco31I	GGTCTC	2 cut(s)	755, 1297
Eco32I	GATATC	1 cut(s)	178
Eco47I	GGWCC	3 cut(s)	1199, 1424, 1754
Eco53kI	GAGCTC	2 cut(s)	325, 593
Eco57I	CTGAAG	2 cut(s)	1134, 1516
Eco88I	CYCGRG	1 cut(s)	758
EcoICRI	GAGCTC	2 cut(s)	325, 593
EcoRI	GAATTC	1 cut(s)	1536
EcoRII	CCWGG	1 cut(s)	1408
EcoRV	GATATC	1 cut(s)	178
EcoT14I	CCWWGG	1 cut(s)	742
EcoT22I	ATGCAT	1 cut(s)	432
EcoT38I	GRGCYC	2 cut(s)	327, 595
ErhI	CCWWGG	1 cut(s)	742
FaeI	CATG	7 cut(s)	176, 277, 331, 1105, 1229, 1595, 1654
FaqI	GGGAC	2 cut(s)	1123, 1250
FatI	CATG	7 cut(s)	172, 273, 327, 1101, 1225, 1591, 1650
FauI	CCCGC	3 cut(s)	383, 1692, 1699
FbaI	TGATCA	2 cut(s)	13, 729
Fnu4HI	GCNGC	6 cut(s)	126, 658, 1632, 1767, 1770, 1785
FokI	GGATG	3 cut(s)	231, 1065, 1492
FriOI	GRGCYC	2 cut(s)	327, 595
Fsp4HI	GCNGC	6 cut(s)	126, 658, 1632, 1767, 1770, 1785
FspBI	CTAG	6 cut(s)	518, 863, 932, 956, 1070, 1326
GlaI	GCGC	2 cut(s)	872, 1740
GluI	GCNGC	6 cut(s)	126, 658, 1632, 1767, 1770, 1785
GsuI	CTGGAG	5 cut(s)	579, 792, 1197, 1584, 1821
HaeIII	GGCC	3 cut(s)	1149, 1381, 1655
HapII	CCGG	5 cut(s)	698, 1060, 1202, 1646, 1792
HhaI	GCGC	2 cut(s)	873, 1741
Hin1II	CATG	7 cut(s)	176, 277, 331, 1105, 1229, 1595, 1654
Hin6I	GCGC	2 cut(s)	871, 1739
HinP1I	GCGC	2 cut(s)	871, 1739
HinfI	GANTC	5 cut(s)	82, 332, 1026, 1586, 1721
HpaII	CCGG	5 cut(s)	698, 1060, 1202, 1646, 1792
HphI	GGTGA	5 cut(s)	8, 43, 74, 335, 1781
Hpy166II	GTNNAC	3 cut(s)	627, 1199, 1252
Hpy188I	TCNGA	6 cut(s)	196, 337, 1137, 1157, 1476, 1535
Hpy8I	GTNNAC	3 cut(s)	627, 1199, 1252
HpyAV	CCTTC	8 cut(s)	94, 294, 395, 781, 987, 1109, 1244, 1453
HpyCH4III	ACNGT	4 cut(s)	1249, 1264, 1281, 1620
HpyCH4IV	ACGT	1 cut(s)	412
HpyF10VI	GCNNNNNNNGC	4 cut(s)	527, 926, 1775, 1784
HpyF3I	CTNAG	6 cut(s)	230, 921, 1083, 1506, 1627, 1746
HpySE526I	ACGT	1 cut(s)	412
Hsp92II	CATG	7 cut(s)	176, 277, 331, 1105, 1229, 1595, 1654
HspAI	GCGC	2 cut(s)	871, 1739
Ksp22I	TGATCA	2 cut(s)	13, 729
LmnI	GCTCC	3 cut(s)	28, 598, 1802
Lsp1109I	GCAGC	5 cut(s)	137, 644, 1643, 1771, 1781
LweI	GCATC	2 cut(s)	187, 669
MaeI	CTAG	6 cut(s)	518, 863, 932, 956, 1070, 1326
MaeII	ACGT	1 cut(s)	412
MaeIII	GTNAC	3 cut(s)	408, 1027, 1339
MbiI	CCGCTC	1 cut(s)	757
MboII	GAAGA	5 cut(s)	332, 1009, 1424, 1522, 1632
MfeI	CAATTG	1 cut(s)	636
MflI	RGATCY	4 cut(s)	207, 337, 454, 856
MhlI	GDGCHC	3 cut(s)	189, 327, 595
MlsI	TGGCCA	1 cut(s)	1655
MluNI	TGGCCA	1 cut(s)	1655
MlyI	GAGTC	2 cut(s)	341, 1035
MmeI	TCCRAC	2 cut(s)	569, 1402
Mox20I	TGGCCA	1 cut(s)	1655
Mph1103I	ATGCAT	1 cut(s)	432
MroXI	GAANNNNTTC	2 cut(s)	296, 384
MscI	TGGCCA	1 cut(s)	1655
MseI	TTAA	7 cut(s)	222, 239, 264, 459, 534, 1092, 1709
Msp20I	TGGCCA	1 cut(s)	1655
MspA1I	CMGCKG	1 cut(s)	1772
MspCI	CTTAAG	2 cut(s)	458, 1091
MspI	CCGG	5 cut(s)	698, 1060, 1202, 1646, 1792
MspR9I	CCNGG	4 cut(s)	699, 1061, 1410, 1647
MunI	CAATTG	1 cut(s)	636
MvaI	CCWGG	1 cut(s)	1410
MvnI	CGCG	2 cut(s)	378, 1739
MwoI	GCNNNNNNNGC	4 cut(s)	527, 926, 1775, 1784
NciI	CCSGG	3 cut(s)	699, 1061, 1647
NlaIII	CATG	7 cut(s)	176, 277, 331, 1105, 1229, 1595, 1654
NlaIV	GGNNCC	2 cut(s)	1395, 1765
NmeAIII	GCCGAG	1 cut(s)	694
NmuCI	GTSAC	1 cut(s)	1027
NsiI	ATGCAT	1 cut(s)	432
NspI	RCATGY	2 cut(s)	1229, 1595
NspV	TTCGAA	1 cut(s)	995
PaeR7I	CTCGAG	1 cut(s)	758
PaqCI	CACCTGC	1 cut(s)	640
PdmI	GAANNNNTTC	2 cut(s)	296, 384
PfeI	GAWTC	3 cut(s)	82, 1586, 1721
PinAI	ACCGGT	1 cut(s)	1201
PkrI	GCNGC	6 cut(s)	127, 659, 1633, 1768, 1771, 1786
PleI	GAGTC	2 cut(s)	340, 1034
PpsI	GAGTC	2 cut(s)	340, 1034
Ppu21I	YACGTR	1 cut(s)	413
Psp124BI	GAGCTC	2 cut(s)	327, 595
Psp6I	CCWGG	1 cut(s)	1408
PspGI	CCWGG	1 cut(s)	1408
PspN4I	GGNNCC	2 cut(s)	1395, 1765
PspPI	GGNCC	3 cut(s)	1199, 1424, 1754
PsrI	GAACNNNNNNTAC	2 cut(s)	1664, 1696
PsuI	RGATCY	4 cut(s)	207, 337, 454, 856
PvuII	CAGCTG	1 cut(s)	1772
RsaI	GTAC	2 cut(s)	626, 1261
RsaNI	GTAC	2 cut(s)	625, 1260
SacI	GAGCTC	2 cut(s)	327, 595
SaqAI	TTAA	7 cut(s)	222, 239, 264, 459, 534, 1092, 1709
SatI	GCNGC	6 cut(s)	126, 658, 1632, 1767, 1770, 1785
Sau96I	GGNCC	3 cut(s)	1199, 1424, 1754
SchI	GAGTC	2 cut(s)	341, 1035
ScrFI	CCNGG	4 cut(s)	699, 1061, 1410, 1647
SduI	GDGCHC	3 cut(s)	189, 327, 595
SfaNI	GCATC	2 cut(s)	187, 669
SfcI	CTRYAG	3 cut(s)	1758, 1773, 1779
Sfr274I	CTCGAG	1 cut(s)	758
SfuI	TTCGAA	1 cut(s)	995
SinI	GGWCC	3 cut(s)	1199, 1424, 1754
SlaI	CTCGAG	1 cut(s)	758
SmlI	CTYRAG	5 cut(s)	458, 758, 1091, 1175, 1255
SmoI	CTYRAG	5 cut(s)	458, 758, 1091, 1175, 1255
SsiI	CCGC	6 cut(s)	268, 376, 755, 1685, 1692, 1767
SspI	AATATT	1 cut(s)	555
SspMI	CTAG	6 cut(s)	518, 863, 932, 956, 1070, 1326
SstI	GAGCTC	2 cut(s)	327, 595
StyD4I	CCNGG	4 cut(s)	697, 1059, 1408, 1645
StyI	CCWWGG	1 cut(s)	742
TaaI	ACNGT	4 cut(s)	1249, 1264, 1281, 1620
TaiI	ACGT	1 cut(s)	415
TaqI	TCGA	5 cut(s)	87, 451, 759, 995, 1715
TatI	WGTACW	2 cut(s)	624, 1259
TauI	GCSGC	1 cut(s)	1769
TfiI	GAWTC	3 cut(s)	82, 1586, 1721
Tru1I	TTAA	7 cut(s)	222, 239, 264, 459, 534, 1092, 1709
Tru9I	TTAA	7 cut(s)	222, 239, 264, 459, 534, 1092, 1709
TscAI	CASTG	5 cut(s)	739, 873, 1529, 1625, 1804
TseFI	GTSAC	1 cut(s)	1027
TseI	GCWGC	5 cut(s)	125, 657, 1631, 1769, 1784
Tsp45I	GTSAC	1 cut(s)	1027
TspDTI	ATGAA	4 cut(s)	704, 1118, 1529, 1599
TspGWI	ACGGA	1 cut(s)	1358
TspRI	CASTG	5 cut(s)	739, 873, 1529, 1625, 1804
Vha464I	CTTAAG	2 cut(s)	458, 1091
VpaK11BI	GGWCC	3 cut(s)	1199, 1424, 1754
XapI	RAATTY	5 cut(s)	842, 1004, 1010, 1285, 1536
XceI	RCATGY	2 cut(s)	1229, 1595
XhoI	CTCGAG	1 cut(s)	758
XmnI	GAANNNNTTC	2 cut(s)	296, 384
XspI	CTAG	6 cut(s)	518, 863, 932, 956, 1070, 1326
Zsp2I	ATGCAT	1 cut(s)	432

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.