Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002563

Physical Location & Seq

Genomic Coordinates Forward (+)

12538046 .. 12543510

Gene Structure 5465 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1139400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1857 bp

ATGCAGCAGGATCACTCCAAGAAGAATCAAACAGAGATGGACTTCTTCTCGGATTACGGTGATGCCAATCGGTACAAAATTCAGGAAGTTATCGGGAAGGGAAGCTATGGTGTTGTTTGCTCAGCAATAGACACACATACGGGAGAAAAAGTGGCAATAAAAAAAATACATGACATTTTTGAGCATATATCTGATGCCGCTCGTATTCTTCGTGAGATAAAGCTTCTCAGACTTCTACGACATCCCGACATTGTTGAAATTAAGCACATTATGCTACCACCTTCTCGAAGAGACTTTAAAGACATCTATGTTGTTTTTGAGCTCATGGAATCAGATCTTCATCAAGTTATCAAAGCTAATGATGATCTGACACGAGAACACTATCAGTTCTTTCTTTACCAGCTGCTTCGTGCATTGAAGTATATTCACACCGCAAATGTCTACCATCGAGATTTGAAACCGAAGAACATATTGGCAAATGCAAATTGTAAGCTTAAAATTTGCGATTTTGGGCTAGCAAGAGTTGCTTTCAATGATACGCCTACGACAATATTTTGGACAGATTATGTTGCAACTAGATGGTATAGAGCTCCAGAGCTCTGTGGATCCTTTTTCTCTAAGTATACCCCTGCGATTGATGTATGGAGTATAGGCTGCATCTTTGCTGAGGTATTGACAGGGAAGCCACTTTTTCCTGGAAAGAATGTTGTTCACCAGCTGGATTTGATGACTGATCTTCTTGGGACACCATCACTAGATACCATATCCCGGGTTCGAAATGAAAAGGCAAGGAGATACTTAACCAGTATGAGGAAAAAACAACCTGTGTGCTTCGCACAAAAATTTCCAAATGCTGATCCTTTAGCACTACGACTTCTGGAAAGATTGCTTGCCTTTGATCCCAAGGACCGTCCAACTGCCCAAGAGGCACTCGCTGATCCTTATTTTAAGGGACTGTCAAGAGTTGAGAGGGAACCATCGTGCCAGCCAATTACGAAGATGGAGTTTGAATTTGAAAGGCGAAGGGTCACAAAGGAAGACATACGAGAGTTAATTTTCCGGGAGATATTGGAGTACCATCCTCAATTGCTAAAAGACTACATAAATGGAACTGAGAGAACTAATTTTCTCTATCCAAGTGCTGTGGATCAATTTAGAAAGCAGTTTGCACACCTTGAGGAGAATGGGGGTAAAAGTGGTCCAGTTATACCGCTTGAACGAAAGCACGTGTCACTTCCTAGATCCACAATTGTACATTCAAATACGGTCCCTCCTAAAGAGCAACAAAATTATGCATTTTTGAAAGATCAGAAAAATGCTGAGGAGGCTTACAGGAATTCAAGGGATTCGGAAGCAGTTCATGTAAATATATCAAGAACCATGCAAACACCACAAAGAATTTCTTTGGCCAAACCAGGAAGAGTTGTTGGACCTGTTGCACAATGCGATAATGGGAGCATGATGAAAGATGCCTATGACCGGAGGACACTAGTTAGAGGTTCGGTACTTCCCCCTCAGGCTGTCCCTCCTGCTTATTGTTACCGCAAGCCCAGTGCTGGAAATCAAGAAAGATCTGCAGTGGAAGGTGAGAGGGACTTGTCCTCTCAAGCCAAGCAAGCTGCACATTGTGGCATGGCAGCGAAAGTGGCACCAGATGTAGCTATCAGCATAGACTCCAACCCATTTTTTATGACTCGGGTGGGAGTCCCTAAAGTGGAACATCATGATCGAATTGCTATTGACACCCACTACCTGCAGACAAAGGCTCCATATGGTGGGATTGACCCTGCTGCTACCACTGCAGCAGCCCACCGTAAGGTTGGAACTGTTCAGTTTGGCATGTCAAGGATGTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000165 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

619

Amino Acids

70.56

Weight (kDa)

9.15

Isoelectric Point (pI)

35.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	25 - 316	1.8e-65	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	26 - 234	6.8e-32	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002563)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53510 AT3G14720 AT3G14720 AT3G14720
fragaria_vesca	FvH4_3g17440 FvH4_3g17440 FvH4_3g17440 FvH4_6g38240 FvH4_6g38240 FvH4_6g38240 FvH4_6g38240 FvH4_6g38240
malus_domestica	MD03G1251600.v1.1 MD09G1151800.v1.1 MD11G1272700.v1.1 MD17G1139400.v1.1
prunus_persica	Prupe.3G172800_v2.0.a1 Prupe.3G172800_v2.0.a1 Prupe.4G154700_v2.0.a1
pyrus_communis	pycom03g19900
rosa_chinensis	RchiOBHm_Chr2g0151501 RchiOBHm_Chr5g0028971
rosa_laevigata	RLG00000020555 RLG00000033132
rosa_multiflora	Rmu_sc0000940.1_g000020 Rmu_sc0010209.1_g000001
rosa_roxburghii	Rroxscaffold_1G00050920 Rroxscaffold_2G00096400
rosa_rugosa	Rorug02G0426600 Rorug05G0112100
rosa_samantha	Rh2AG487400 Rh2BG499800 Rh2CG473800 Rh2DG511000 Rh5AG204100 Rh5BG202400 Rh5CG223200 Rh5DG206200
rosa_wichuraiana	Rw2G039950 Rw5G018500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1761
AccB1I	GGYRCC	1 cut(s)	1648
AccB7I	CCANNNNNTGG	1 cut(s)	1775
AccBSI	CCGCTC	1 cut(s)	200
AccI	GTMKAC	2 cut(s)	441, 623
AciI	CCGC	4 cut(s)	198, 432, 1211, 1543
AclWI	GGATC	8 cut(s)	18, 600, 613, 851, 893, 932, 1155, 1236
AcoI	YGGCCR	1 cut(s)	1407
AcsI	RAATTY	6 cut(s)	78, 498, 842, 1010, 1336, 1398
AcvI	CACGTG	1 cut(s)	1228
AdeI	CACNNNGTG	1 cut(s)	1628
AfaI	GTAC	4 cut(s)	74, 1076, 1254, 1506
AfiI	CCNNNNNNNGG	7 cut(s)	768, 810, 1479, 1556, 1714, 1775, 1816
AflIII	ACRYGT	1 cut(s)	1227
AhlI	ACTAGT	1 cut(s)	1489
AjnI	CCWGG	2 cut(s)	694, 1414
AjuI	GAANNNNNNNTTGG	2 cut(s)	455, 487
Alw21I	GWGCWC	3 cut(s)	324, 592, 600
Alw26I	GTCTC	1 cut(s)	285
AlwI	GGATC	8 cut(s)	18, 600, 613, 851, 893, 932, 1155, 1236
Ama87I	CYCGRG	2 cut(s)	768, 1695
AoxI	GGCC	1 cut(s)	1407
ApeKI	GCWGC	8 cut(s)	4, 403, 654, 1619, 1637, 1790, 1802, 1805
ApoI	RAATTY	6 cut(s)	78, 498, 842, 1010, 1336, 1398
Asp700I	GAANNNNTTC	1 cut(s)	1356
AspS9I	GGNCC	4 cut(s)	907, 1199, 1267, 1430
AsuC2I	CCSGG	3 cut(s)	769, 770, 1061
AsuHPI	GGTGA	3 cut(s)	71, 704, 1598
AsuII	TTCGAA	1 cut(s)	775
AsuNHI	GCTAGC	1 cut(s)	514
AvaI	CYCGRG	2 cut(s)	768, 1695
AvaII	GGWCC	4 cut(s)	907, 1199, 1267, 1430
AxyI	CCTNAGG	1 cut(s)	1515
BalI	TGGCCA	1 cut(s)	1409
BamHI	GGATCC	1 cut(s)	605
BanI	GGYRCC	1 cut(s)	1648
BanII	GRGCYC	3 cut(s)	324, 592, 600
BauI	CACGAG	1 cut(s)	372
BbrPI	CACGTG	1 cut(s)	1228
BbsI	GAAGAC	1 cut(s)	1044
Bbv12I	GWGCWC	3 cut(s)	324, 592, 600
BbvCI	CCTCAGC	2 cut(s)	666, 1320
BbvI	GCAGC	8 cut(s)	16, 390, 641, 1606, 1649, 1777, 1814, 1817
BccI	CCATC	7 cut(s)	31, 453, 573, 757, 985, 994, 1086
BciT130I	CCWGG	2 cut(s)	696, 1416
BcnI	CCSGG	3 cut(s)	769, 770, 1061
BcoDI	GTCTC	1 cut(s)	285
BcuI	ACTAGT	1 cut(s)	1489
BfaI	CTAG	5 cut(s)	515, 576, 755, 1239, 1490
BfmI	CTRYAG	3 cut(s)	1575, 1754, 1800
BfuAI	ACCTGC	1 cut(s)	1761
BglI	GCCNNNNNGGC	1 cut(s)	926
BglII	AGATCT	2 cut(s)	334, 1571
BisI	GCNGC	9 cut(s)	5, 198, 404, 655, 1620, 1638, 1791, 1803, 1806
BlpI	GCTNAGC	1 cut(s)	121
BlsI	GCNGC	9 cut(s)	6, 199, 405, 656, 1621, 1639, 1792, 1804, 1807
Bme1390I	CCNGG	5 cut(s)	696, 769, 770, 1061, 1416
Bme18I	GGWCC	4 cut(s)	907, 1199, 1267, 1430
BmeT110I	CYCGRG	2 cut(s)	768, 1695
BmgT120I	GGNCC	4 cut(s)	907, 1199, 1267, 1430
BmiI	GGNNCC	5 cut(s)	607, 975, 1269, 1650, 1767
BmrFI	CCNGG	5 cut(s)	696, 769, 770, 1061, 1416
BmrI	ACTGGG	1 cut(s)	1545
BmsI	GCATC	4 cut(s)	52, 184, 666, 1459
BmtI	GCTAGC	1 cut(s)	518
BmuI	ACTGGG	1 cut(s)	1545
BpiI	GAAGAC	1 cut(s)	1044
BpmI	CTGGAG	1 cut(s)	576
Bpu10I	CCTNAGC	2 cut(s)	666, 1320
Bpu1102I	GCTNAGC	1 cut(s)	121
Bpu14I	TTCGAA	1 cut(s)	775
BpuEI	CTTGAG	2 cut(s)	1196, 1590
BpuMI	CCSGG	3 cut(s)	769, 770, 1061
BsaAI	YACGTR	1 cut(s)	1228
BsaBI	GATNNNNATC	2 cut(s)	66, 339
BsaJI	CCNNGG	2 cut(s)	768, 903
BsaWI	WCCGGW	1 cut(s)	1479
BsaXI	ACNNNNNCTCC	6 cut(s)	135, 165, 1255, 1285, 1750, 1780
Bsc4I	CCNNNNNNNGG	7 cut(s)	768, 810, 1479, 1556, 1714, 1775, 1816
Bse1I	ACTGG	3 cut(s)	804, 1202, 1551
Bse21I	CCTNAGG	1 cut(s)	1515
Bse8I	GATNNNNATC	2 cut(s)	66, 339
BseBI	CCWGG	2 cut(s)	696, 1416
BseDI	CCNNGG	2 cut(s)	768, 903
BseGI	GGATG	3 cut(s)	241, 1078, 1854
BseJI	GATNNNNATC	2 cut(s)	66, 339
BseLI	CCNNNNNNNGG	7 cut(s)	768, 810, 1479, 1556, 1714, 1775, 1816
BseMII	CTCAG	6 cut(s)	135, 241, 657, 1104, 1311, 1529
BseNI	ACTGG	3 cut(s)	804, 1202, 1551
BseRI	GAGGAG	2 cut(s)	1193, 1337
BseXI	GCAGC	8 cut(s)	16, 390, 641, 1606, 1649, 1777, 1814, 1817
BsgI	GTGCAG	1 cut(s)	1605
BshFI	GGCC	1 cut(s)	1409
BshNI	GGYRCC	1 cut(s)	1648
BsiHKAI	GWGCWC	3 cut(s)	324, 592, 600
BsiHKCI	CYCGRG	2 cut(s)	768, 1695
BsiSI	CCGG	3 cut(s)	769, 1060, 1480
BslFI	GGGAC	6 cut(s)	757, 966, 1253, 1508, 1607, 1691
BslI	CCNNNNNNNGG	7 cut(s)	768, 810, 1479, 1556, 1714, 1775, 1816
BsmAI	GTCTC	1 cut(s)	285
BsmFI	GGGAC	6 cut(s)	757, 966, 1253, 1508, 1607, 1691
BsnI	GGCC	1 cut(s)	1409
BsoBI	CYCGRG	2 cut(s)	768, 1695
Bsp119I	TTCGAA	1 cut(s)	775
Bsp1286I	GDGCHC	3 cut(s)	324, 592, 600
Bsp1407I	TGTACA	1 cut(s)	1252
Bsp1720I	GCTNAGC	1 cut(s)	121
BspACI	CCGC	4 cut(s)	198, 432, 1211, 1543
BspANI	GGCC	1 cut(s)	1409
BspCNI	CTCAG	6 cut(s)	134, 240, 658, 1105, 1312, 1528
BspHI	TCATGA	1 cut(s)	1723
BspLI	GGNNCC	5 cut(s)	607, 975, 1269, 1650, 1767
BspMAI	CTGCAG	3 cut(s)	1579, 1758, 1804
BspMI	ACCTGC	1 cut(s)	1761
BspOI	GCTAGC	1 cut(s)	518
BspPI	GGATC	8 cut(s)	18, 600, 613, 851, 893, 932, 1155, 1236
BspT104I	TTCGAA	1 cut(s)	775
BspT107I	GGYRCC	1 cut(s)	1648
BsrBI	CCGCTC	1 cut(s)	200
BsrGI	TGTACA	1 cut(s)	1252
BsrI	ACTGG	3 cut(s)	804, 1202, 1551
BssECI	CCNNGG	2 cut(s)	768, 903
BssNAI	GTATAC	1 cut(s)	624
BssSI	CACGAG	1 cut(s)	372
BssT1I	CCWWGG	1 cut(s)	903
Bst1107I	GTATAC	1 cut(s)	624
Bst2BI	CACGAG	1 cut(s)	372
Bst2UI	CCWGG	2 cut(s)	696, 1416
Bst4CI	ACNGT	6 cut(s)	59, 911, 957, 1267, 1814, 1828
Bst6I	CTCTTC	2 cut(s)	283, 1414
BstAPI	GCANNNNNTGC	2 cut(s)	271, 524
BstAUI	TGTACA	1 cut(s)	1252
BstBAI	YACGTR	1 cut(s)	1228
BstBI	TTCGAA	1 cut(s)	775
BstC8I	GCNNGC	5 cut(s)	516, 891, 986, 1547, 1617
BstDEI	CTNAG	7 cut(s)	121, 227, 618, 666, 1113, 1320, 1515
BstF5I	GGATG	3 cut(s)	241, 1078, 1854
BstMAI	GTCTC	1 cut(s)	285
BstMWI	GCNNNNNNNGC	7 cut(s)	271, 524, 926, 1325, 1616, 1646, 1799
BstNI	CCWGG	2 cut(s)	696, 1416
BstNSI	RCATGY	1 cut(s)	1843
BstSCI	CCNGG	5 cut(s)	694, 767, 768, 1059, 1414
BstSFI	CTRYAG	3 cut(s)	1575, 1754, 1800
BstV1I	GCAGC	8 cut(s)	16, 390, 641, 1606, 1649, 1777, 1814, 1817
BstV2I	GAAGAC	1 cut(s)	1044
BstX2I	RGATCY	4 cut(s)	334, 605, 1241, 1571
BstYI	RGATCY	4 cut(s)	334, 605, 1241, 1571
BstZ17I	GTATAC	1 cut(s)	624
Bsu36I	CCTNAGG	1 cut(s)	1515
BsuRI	GGCC	1 cut(s)	1409
BtsCI	GGATG	3 cut(s)	241, 1078, 1854
BtsI	GCAGTG	2 cut(s)	1584, 1797
BtsIMutI	CAGTG	3 cut(s)	1558, 1584, 1797
BveI	ACCTGC	1 cut(s)	1761
Cac8I	GCNNGC	5 cut(s)	516, 891, 986, 1547, 1617
CciI	TCATGA	1 cut(s)	1723
Cfr13I	GGNCC	4 cut(s)	907, 1199, 1267, 1430
Cfr9I	CCCGGG	1 cut(s)	768
Csp6I	GTAC	4 cut(s)	73, 1075, 1253, 1505
CspCI	CAANNNNNGTGG	2 cut(s)	1125, 1160
CviAII	CATG	8 cut(s)	170, 325, 1361, 1381, 1459, 1633, 1724, 1840
CviQI	GTAC	4 cut(s)	73, 1075, 1253, 1505
DdeI	CTNAG	7 cut(s)	121, 227, 618, 666, 1113, 1320, 1515
DraI	TTTAAA	1 cut(s)	298
DraIII	CACNNNGTG	1 cut(s)	1628
EaeI	YGGCCR	1 cut(s)	1407
Eam1104I	CTCTTC	2 cut(s)	283, 1414
EarI	CTCTTC	2 cut(s)	283, 1414
Ecl136II	GAGCTC	3 cut(s)	322, 590, 598
Eco130I	CCWWGG	1 cut(s)	903
Eco24I	GRGCYC	3 cut(s)	324, 592, 600
Eco47I	GGWCC	4 cut(s)	907, 1199, 1267, 1430
Eco53kI	GAGCTC	3 cut(s)	322, 590, 598
Eco72I	CACGTG	1 cut(s)	1228
Eco81I	CCTNAGG	1 cut(s)	1515
Eco88I	CYCGRG	2 cut(s)	768, 1695
EcoICRI	GAGCTC	3 cut(s)	322, 590, 598
EcoRI	GAATTC	1 cut(s)	1336
EcoRII	CCWGG	2 cut(s)	694, 1414
EcoT14I	CCWWGG	1 cut(s)	903
EcoT22I	ATGCAT	1 cut(s)	1297
EcoT38I	GRGCYC	3 cut(s)	324, 592, 600
ErhI	CCWWGG	1 cut(s)	903
FaeI	CATG	8 cut(s)	173, 328, 1364, 1384, 1462, 1636, 1727, 1843
FalI	AAGNNNNNCTT	4 cut(s)	511, 543, 1387, 1419
FaqI	GGGAC	6 cut(s)	757, 966, 1253, 1508, 1607, 1691
FatI	CATG	8 cut(s)	169, 324, 1360, 1380, 1458, 1632, 1723, 1839
FauNDI	CATATG	1 cut(s)	1771
FblI	GTMKAC	2 cut(s)	441, 623
Fnu4HI	GCNGC	9 cut(s)	5, 198, 404, 655, 1620, 1638, 1791, 1803, 1806
FokI	GGATG	2 cut(s)	228, 1065
FriOI	GRGCYC	3 cut(s)	324, 592, 600
Fsp4HI	GCNGC	9 cut(s)	5, 198, 404, 655, 1620, 1638, 1791, 1803, 1806
FspBI	CTAG	5 cut(s)	515, 576, 755, 1239, 1490
GluI	GCNGC	9 cut(s)	5, 198, 404, 655, 1620, 1638, 1791, 1803, 1806
GsuI	CTGGAG	1 cut(s)	576
HaeIII	GGCC	1 cut(s)	1409
HapII	CCGG	3 cut(s)	769, 1060, 1480
Hin1II	CATG	8 cut(s)	173, 328, 1364, 1384, 1462, 1636, 1727, 1843
HindIII	AAGCTT	2 cut(s)	221, 491
HinfI	GANTC	6 cut(s)	25, 329, 1346, 1673, 1693, 1704
HpaII	CCGG	3 cut(s)	769, 1060, 1480
HphI	GGTGA	3 cut(s)	71, 704, 1598
Hpy166II	GTNNAC	3 cut(s)	442, 624, 712
Hpy188I	TCNGA	7 cut(s)	52, 193, 230, 334, 369, 1311, 1351
Hpy8I	GTNNAC	3 cut(s)	442, 624, 712
HpyAV	CCTTC	4 cut(s)	91, 291, 1017, 1577
HpyCH4III	ACNGT	6 cut(s)	59, 911, 957, 1267, 1814, 1828
HpyCH4IV	ACGT	1 cut(s)	1227
HpyF10VI	GCNNNNNNNGC	7 cut(s)	271, 524, 926, 1325, 1616, 1646, 1799
HpyF3I	CTNAG	7 cut(s)	121, 227, 618, 666, 1113, 1320, 1515
HpySE526I	ACGT	1 cut(s)	1227
Hsp92II	CATG	8 cut(s)	173, 328, 1364, 1384, 1462, 1636, 1727, 1843
LmnI	GCTCC	3 cut(s)	595, 1455, 1771
Lsp1109I	GCAGC	8 cut(s)	16, 390, 641, 1606, 1649, 1777, 1814, 1817
LweI	GCATC	4 cut(s)	52, 184, 666, 1459
MaeI	CTAG	5 cut(s)	515, 576, 755, 1239, 1490
MaeII	ACGT	1 cut(s)	1227
MaeIII	GTNAC	3 cut(s)	1027, 1230, 1538
MbiI	CCGCTC	1 cut(s)	200
MfeI	CAATTG	2 cut(s)	1085, 1248
MflI	RGATCY	4 cut(s)	334, 605, 1241, 1571
MhlI	GDGCHC	3 cut(s)	324, 592, 600
MlsI	TGGCCA	1 cut(s)	1409
MluNI	TGGCCA	1 cut(s)	1409
MlyI	GAGTC	3 cut(s)	1667, 1687, 1713
MmeI	TCCRAC	4 cut(s)	938, 1408, 1701, 1801
Mox20I	TGGCCA	1 cut(s)	1409
Mph1103I	ATGCAT	1 cut(s)	1297
MroXI	GAANNNNTTC	1 cut(s)	1356
MscI	TGGCCA	1 cut(s)	1409
MseI	TTAA	6 cut(s)	261, 297, 495, 800, 948, 1052
Msp20I	TGGCCA	1 cut(s)	1409
MspA1I	CMGCKG	2 cut(s)	403, 718
MspI	CCGG	3 cut(s)	769, 1060, 1480
MspR9I	CCNGG	5 cut(s)	696, 769, 770, 1061, 1416
MunI	CAATTG	2 cut(s)	1085, 1248
MvaI	CCWGG	2 cut(s)	696, 1416
MwoI	GCNNNNNNNGC	7 cut(s)	271, 524, 926, 1325, 1616, 1646, 1799
NciI	CCSGG	3 cut(s)	769, 770, 1061
NdeI	CATATG	1 cut(s)	1771
NheI	GCTAGC	1 cut(s)	514
NlaIII	CATG	8 cut(s)	173, 328, 1364, 1384, 1462, 1636, 1727, 1843
NlaIV	GGNNCC	5 cut(s)	607, 975, 1269, 1650, 1767
NmuCI	GTSAC	2 cut(s)	1027, 1230
NsiI	ATGCAT	1 cut(s)	1297
NspI	RCATGY	1 cut(s)	1843
NspV	TTCGAA	1 cut(s)	775
PagI	TCATGA	1 cut(s)	1723
PcsI	WCGNNNNNNNCGW	1 cut(s)	208
PdmI	GAANNNNTTC	1 cut(s)	1356
PfeI	GAWTC	3 cut(s)	25, 329, 1346
PflMI	CCANNNNNTGG	1 cut(s)	1775
PfoI	TCCNGGA	2 cut(s)	694, 1059
PkrI	GCNGC	9 cut(s)	6, 199, 405, 656, 1621, 1639, 1792, 1804, 1807
PleI	GAGTC	3 cut(s)	1667, 1687, 1712
PmaCI	CACGTG	1 cut(s)	1228
PmlI	CACGTG	1 cut(s)	1228
PpsI	GAGTC	3 cut(s)	1667, 1687, 1712
Ppu21I	YACGTR	1 cut(s)	1228
Psp124BI	GAGCTC	3 cut(s)	324, 592, 600
Psp6I	CCWGG	2 cut(s)	694, 1414
PspCI	CACGTG	1 cut(s)	1228
PspGI	CCWGG	2 cut(s)	694, 1414
PspN4I	GGNNCC	5 cut(s)	607, 975, 1269, 1650, 1767
PspPI	GGNCC	4 cut(s)	907, 1199, 1267, 1430
PstI	CTGCAG	3 cut(s)	1579, 1758, 1804
PsuI	RGATCY	4 cut(s)	334, 605, 1241, 1571
PvuII	CAGCTG	2 cut(s)	403, 718
RsaI	GTAC	4 cut(s)	74, 1076, 1254, 1506
RsaNI	GTAC	4 cut(s)	73, 1075, 1253, 1505
SacI	GAGCTC	3 cut(s)	324, 592, 600
SaqAI	TTAA	6 cut(s)	261, 297, 495, 800, 948, 1052
SatI	GCNGC	9 cut(s)	5, 198, 404, 655, 1620, 1638, 1791, 1803, 1806
Sau96I	GGNCC	4 cut(s)	907, 1199, 1267, 1430
SchI	GAGTC	3 cut(s)	1667, 1687, 1713
ScrFI	CCNGG	5 cut(s)	696, 769, 770, 1061, 1416
SduI	GDGCHC	3 cut(s)	324, 592, 600
SfaNI	GCATC	4 cut(s)	52, 184, 666, 1459
SfcI	CTRYAG	3 cut(s)	1575, 1754, 1800
SfuI	TTCGAA	1 cut(s)	775
SinI	GGWCC	4 cut(s)	907, 1199, 1267, 1430
SmaI	CCCGGG	1 cut(s)	770
SmlI	CTYRAG	2 cut(s)	1175, 1605
SmoI	CTYRAG	2 cut(s)	1175, 1605
SpeI	ACTAGT	1 cut(s)	1489
SsiI	CCGC	4 cut(s)	198, 432, 1211, 1543
SspI	AATATT	1 cut(s)	552
SspMI	CTAG	5 cut(s)	515, 576, 755, 1239, 1490
SstI	GAGCTC	3 cut(s)	324, 592, 600
StyD4I	CCNGG	5 cut(s)	694, 767, 768, 1059, 1414
StyI	CCWWGG	1 cut(s)	903
TaaI	ACNGT	6 cut(s)	59, 911, 957, 1267, 1814, 1828
TaiI	ACGT	1 cut(s)	1230
TaqI	TCGA	4 cut(s)	286, 448, 775, 1729
TatI	WGTACW	1 cut(s)	1252
TauI	GCSGC	1 cut(s)	200
TfiI	GAWTC	3 cut(s)	25, 329, 1346
Tru1I	TTAA	6 cut(s)	261, 297, 495, 800, 948, 1052
Tru9I	TTAA	6 cut(s)	261, 297, 495, 800, 948, 1052
TscAI	CASTG	3 cut(s)	1558, 1584, 1804
TseFI	GTSAC	2 cut(s)	1027, 1230
TseI	GCWGC	8 cut(s)	4, 403, 654, 1619, 1637, 1790, 1802, 1805
Tsp45I	GTSAC	2 cut(s)	1027, 1230
TspDTI	ATGAA	4 cut(s)	329, 795, 1349, 1478
TspMI	CCCGGG	1 cut(s)	768
TspRI	CASTG	3 cut(s)	1558, 1584, 1804
Van91I	CCANNNNNTGG	1 cut(s)	1775
VpaK11BI	GGWCC	4 cut(s)	907, 1199, 1267, 1430
XapI	RAATTY	6 cut(s)	78, 498, 842, 1010, 1336, 1398
XceI	RCATGY	1 cut(s)	1843
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1817
XmaI	CCCGGG	1 cut(s)	768
XmiI	GTMKAC	2 cut(s)	441, 623
XmnI	GAANNNNTTC	1 cut(s)	1356
XspI	CTAG	5 cut(s)	515, 576, 755, 1239, 1490
Zsp2I	ATGCAT	1 cut(s)	1297

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.