Rh2CG473800

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002563
Physical Location & Seq
Reverse (-)
63900685 .. 63905622
4938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG473800.1

Sequence Viewer

Length: 1248 bp
ATGGTGAGCAATTATACACCTGCAATTGATATATGGAGTATAGGCTGCATCTTTGCTGAAGTATTAACTGGAAAGCCCCTCTTTCCTGGAAAGAATGTTGTTCACCAGCTGGATTTGATGACTGATCTGCTTGGAACACCCTCATTGGATACCATATCTCGGGTTCGAAACGATAAGGCAAGGAGATACCTAACAAGTATGAGAAAAAAGCAACCTGTGTCGTTTGCACAGAAATTTCCAAATGCTGATCCTTTAGCACTACGGCTTTTGCAAAGATTGCTTGCCTTCGATCCTAAGGACCGACCAACTGCCGAAGAGGCACTGGCTGATCCTTATTTTAAGGGACTCTCAAAAATAGAGAGGGAGCCTTCATGCCAGCCAATTACGAAGATGGAGTTTGAATTTGAGAGGCGAAGGGTCACAAAGGAGGATATACGGGAGCTTATTTTTCGGGAAATATTGGAGTACCATCCTCAATTGCTTAAAGACTACATAAATGGAACCGAAAGGACTAATTTTCTCTATCCAAGTGCTGTTGATCAATTCCGGAAGCAGTTTGCACATCTGGAGGAAAATAGTGGTAAAAGTGCCCCAGTTATTCCACTTGAACGAAAGCATGTATCTCTTCCTAGGTCTACAATTGTACACTCAAATACGGTGCCCCCTAAGGAACAACAAAATTATGCATACTTGAAAGATCAGAAACACGCTGAGGAGGCATACAAGAATTCAAGGGATACAGAAGGAATACATGTAAATCTATCACGAACCATGCAAGCACCACAAAGAATTCCCTTAGCTAAACCCGGTAGAGTTGTTGGGCCTGTTGTACCATATGAGAATGGAAATATTGTCAAAGATGCTTATGACCGAAGGACGCTTATTAGAAGTACAGTACTGCCCCCTCAGGCTGTTCCTCCGTCTTATTGTTACCGGAAACCCAGTGGTGGAAGTCAAGAAAGGTCTGCAGTGGAAGTGAATAGAGACTTTTCCTCGCAGTCCAAGCAAGCTGCACAGTGTGGCATGGCAACAAAAGTAGCACCGGATGTAGCAATCAACATTGACACAAACCCATTTTTTATGACACGGGTGGGAGTCAGCAAGGTGGAACATGATGACCGAATTGCTATCGACACAAACTTCTTGCAGTCAAAGGTTCCATATGGTGGGATTGGTTCTGCTGCTGCCACTGCAGCTGCCCACCGCAAGGTTGGAACTGTTCAGTTTGGCATGTCAAGGATGTACTAG

Protein Analysis

415

Amino Acids

46.79

Weight (kDa)

9.44

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 113 2.5e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 28
Acc36I ACCTGC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 658
AccB7I CCANNNNNTGG 1 cut(s) 1166
AccI GTMKAC 1 cut(s) 635
AccIII TCCGGA 1 cut(s) 546
AciI CCGC 1 cut(s) 1204
AclWI GGATC 3 cut(s) 242, 284, 323
AcsI RAATTY 4 cut(s) 233, 401, 727, 789
AcuI CTGAAG 1 cut(s) 78
AdeI CACNNNGTG 1 cut(s) 1019
AfaI GTAC 6 cut(s) 467, 645, 831, 892, 897, 1244
AfiI CCNNNNNNNGG 4 cut(s) 159, 947, 1166, 1207
AflIII ACRYGT 1 cut(s) 751
AgsI TTSAA 4 cut(s) 401, 608, 694, 732
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 5 cut(s) 109, 442, 800, 1010, 1196
AluI AGCT 5 cut(s) 109, 442, 800, 1010, 1196
Alw26I GTCTC 1 cut(s) 978
AlwI GGATC 3 cut(s) 242, 284, 323
Ama87I CYCGRG 1 cut(s) 159
Aor13HI TCCGGA 1 cut(s) 546
AoxI GGCC 1 cut(s) 821
ApeKI GCWGC 6 cut(s) 45, 1010, 1181, 1184, 1193, 1196
ApoI RAATTY 4 cut(s) 233, 401, 727, 789
AspA2I CCTAGG 1 cut(s) 629
AspS9I GGNCC 2 cut(s) 298, 821
AsuC2I CCSGG 1 cut(s) 807
AsuHPI GGTGA 2 cut(s) 16, 95
AsuII TTCGAA 1 cut(s) 166
AvaI CYCGRG 1 cut(s) 159
AvaII GGWCC 1 cut(s) 298
AvrII CCTAGG 1 cut(s) 629
AxyI CCTNAGG 3 cut(s) 294, 666, 906
BaeGI GKGCMC 2 cut(s) 592, 663
BanI GGYRCC 1 cut(s) 658
BbvCI CCTCAGC 1 cut(s) 711
BbvI GCAGC 6 cut(s) 32, 997, 1168, 1171, 1183, 1205
BccI CCATC 2 cut(s) 385, 477
BceAI ACGGC 1 cut(s) 278
BciT130I CCWGG 1 cut(s) 87
BciVI GTATCC 2 cut(s) 142, 730
BclI TGATCA 1 cut(s) 538
BcnI CCSGG 1 cut(s) 807
BcoDI GTCTC 1 cut(s) 978
BfaI CTAG 2 cut(s) 630, 1246
BfmI CTRYAG 2 cut(s) 966, 1191
BfuAI ACCTGC 1 cut(s) 28
BfuI GTATCC 2 cut(s) 142, 730
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 6 cut(s) 46, 1011, 1182, 1185, 1194, 1197
BlnI CCTAGG 1 cut(s) 629
BlsI GCNGC 6 cut(s) 47, 1012, 1183, 1186, 1195, 1198
BmcAI AGTACT 1 cut(s) 897
Bme1390I CCNGG 2 cut(s) 87, 807
Bme18I GGWCC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 159
BmgT120I GGNCC 2 cut(s) 298, 821
BmiI GGNNCC 4 cut(s) 366, 502, 660, 1158
BmrFI CCNGG 2 cut(s) 87, 807
BmrI ACTGGG 2 cut(s) 587, 936
BmsI GCATC 2 cut(s) 57, 850
BmuI ACTGGG 2 cut(s) 587, 936
BpmI CTGGAG 1 cut(s) 587
Bpu10I CCTNAGC 2 cut(s) 711, 796
Bpu14I TTCGAA 1 cut(s) 166
BpuMI CCSGG 1 cut(s) 807
BsaJI CCNNGG 1 cut(s) 629
BsaWI WCCGGW 3 cut(s) 546, 933, 1042
Bsc4I CCNNNNNNNGG 4 cut(s) 159, 947, 1166, 1207
Bse1I ACTGG 4 cut(s) 73, 327, 593, 942
Bse21I CCTNAGG 3 cut(s) 294, 666, 906
BseAI TCCGGA 1 cut(s) 546
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 629
BseGI GGATG 3 cut(s) 469, 1051, 1245
BseLI CCNNNNNNNGG 4 cut(s) 159, 947, 1166, 1207
BseMII CTCAG 2 cut(s) 702, 920
BseNI ACTGG 4 cut(s) 73, 327, 593, 942
BseRI GAGGAG 1 cut(s) 728
BseSI GKGCMC 2 cut(s) 592, 663
BseXI GCAGC 6 cut(s) 32, 997, 1168, 1171, 1183, 1205
BsgI GTGCAG 1 cut(s) 996
BshFI GGCC 1 cut(s) 823
BshNI GGYRCC 1 cut(s) 658
BsiHKCI CYCGRG 1 cut(s) 159
BsiSI CCGG 4 cut(s) 547, 807, 934, 1043
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 4 cut(s) 159, 947, 1166, 1207
BsmAI GTCTC 1 cut(s) 978
BsmFI GGGAC 1 cut(s) 357
BsnI GGCC 1 cut(s) 823
BsoBI CYCGRG 1 cut(s) 159
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 2 cut(s) 592, 663
Bsp13I TCCGGA 1 cut(s) 546
Bsp1407I TGTACA 1 cut(s) 643
Bsp143I GATC 6 cut(s) 124, 247, 289, 328, 538, 697
BspACI CCGC 1 cut(s) 1204
BspANI GGCC 1 cut(s) 823
BspCNI CTCAG 2 cut(s) 703, 919
BspEI TCCGGA 1 cut(s) 546
BspLI GGNNCC 4 cut(s) 366, 502, 660, 1158
BspMAI CTGCAG 2 cut(s) 970, 1195
BspMI ACCTGC 1 cut(s) 28
BspPI GGATC 3 cut(s) 242, 284, 323
BspT104I TTCGAA 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 658
BsrGI TGTACA 1 cut(s) 643
BsrI ACTGG 4 cut(s) 73, 327, 593, 942
BssECI CCNNGG 1 cut(s) 629
BssMI GATC 6 cut(s) 124, 247, 289, 328, 538, 697
BssT1I CCWWGG 1 cut(s) 629
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 4 cut(s) 658, 895, 1017, 1219
Bst6I CTCTTC 2 cut(s) 309, 630
BstAPI GCANNNNNTGC 1 cut(s) 277
BstAUI TGTACA 1 cut(s) 643
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 4 cut(s) 282, 377, 777, 1008
BstDEI CTNAG 5 cut(s) 294, 666, 711, 796, 906
BstF5I GGATG 3 cut(s) 469, 1051, 1245
BstKTI GATC 6 cut(s) 127, 250, 292, 331, 541, 700
BstMAI GTCTC 1 cut(s) 978
BstMBI GATC 6 cut(s) 124, 247, 289, 328, 538, 697
BstMWI GCNNNNNNNGC 6 cut(s) 277, 317, 716, 1003, 1190, 1193
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 3 cut(s) 620, 755, 1234
BstSCI CCNGG 2 cut(s) 85, 805
BstSFI CTRYAG 2 cut(s) 966, 1191
BstSLI GKGCMC 2 cut(s) 592, 663
BstV1I GCAGC 6 cut(s) 32, 997, 1168, 1171, 1183, 1205
Bsu36I CCTNAGG 3 cut(s) 294, 666, 906
BsuI GTATCC 2 cut(s) 142, 730
BsuRI GGCC 1 cut(s) 823
BtsCI GGATG 3 cut(s) 469, 1051, 1245
BtsI GCAGTG 2 cut(s) 975, 1188
BtsIMutI CAGTG 5 cut(s) 320, 949, 975, 1022, 1188
BveI ACCTGC 1 cut(s) 28
Cac8I GCNNGC 4 cut(s) 282, 377, 777, 1008
Cfr13I GGNCC 2 cut(s) 298, 821
CseI GACGC 1 cut(s) 886
Csp6I GTAC 6 cut(s) 466, 644, 830, 891, 896, 1243
CviAII CATG 7 cut(s) 372, 617, 752, 772, 1024, 1112, 1231
CviQI GTAC 6 cut(s) 466, 644, 830, 891, 896, 1243
DdeI CTNAG 5 cut(s) 294, 666, 711, 796, 906
DpnI GATC 6 cut(s) 126, 249, 291, 330, 540, 699
DpnII GATC 6 cut(s) 124, 247, 289, 328, 538, 697
DraIII CACNNNGTG 1 cut(s) 1019
Eam1104I CTCTTC 2 cut(s) 309, 630
EarI CTCTTC 2 cut(s) 309, 630
Eco130I CCWWGG 1 cut(s) 629
Eco47I GGWCC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 78
Eco81I CCTNAGG 3 cut(s) 294, 666, 906
Eco88I CYCGRG 1 cut(s) 159
EcoRI GAATTC 2 cut(s) 727, 789
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 629
EcoT22I ATGCAT 1 cut(s) 688
ErhI CCWWGG 1 cut(s) 629
FaeI CATG 7 cut(s) 375, 620, 755, 775, 1027, 1115, 1234
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FaqI GGGAC 1 cut(s) 357
FatI CATG 7 cut(s) 371, 616, 751, 771, 1023, 1111, 1230
FauNDI CATATG 2 cut(s) 835, 1162
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 1 cut(s) 635
Fnu4HI GCNGC 6 cut(s) 46, 1011, 1182, 1185, 1194, 1197
FokI GGATG 2 cut(s) 456, 1058
Fsp4HI GCNGC 6 cut(s) 46, 1011, 1182, 1185, 1194, 1197
FspBI CTAG 2 cut(s) 630, 1246
GluI GCNGC 6 cut(s) 46, 1011, 1182, 1185, 1194, 1197
GsuI CTGGAG 1 cut(s) 587
HaeIII GGCC 1 cut(s) 823
HapII CCGG 4 cut(s) 547, 807, 934, 1043
HgaI GACGC 1 cut(s) 886
Hin1II CATG 7 cut(s) 375, 620, 755, 775, 1027, 1115, 1234
HinfI GANTC 2 cut(s) 345, 1095
HpaII CCGG 4 cut(s) 547, 807, 934, 1043
HphI GGTGA 2 cut(s) 16, 95
Hpy166II GTNNAC 3 cut(s) 103, 636, 646
Hpy188I TCNGA 1 cut(s) 702
Hpy188III TCNNGA 5 cut(s) 452, 547, 566, 765, 956
Hpy8I GTNNAC 3 cut(s) 103, 636, 646
HpyAV CCTTC 5 cut(s) 295, 378, 408, 737, 867
HpyCH4III ACNGT 4 cut(s) 658, 895, 1017, 1219
HpyF10VI GCNNNNNNNGC 6 cut(s) 277, 317, 716, 1003, 1190, 1193
HpyF3I CTNAG 5 cut(s) 294, 666, 711, 796, 906
Hsp92II CATG 7 cut(s) 375, 620, 755, 775, 1027, 1115, 1234
Kpn2I TCCGGA 1 cut(s) 546
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 6 cut(s) 124, 247, 289, 328, 538, 697
LmnI GCTCC 2 cut(s) 364, 439
Lsp1109I GCAGC 6 cut(s) 32, 997, 1168, 1171, 1183, 1205
LweI GCATC 2 cut(s) 57, 850
MaeI CTAG 2 cut(s) 630, 1246
MaeIII GTNAC 2 cut(s) 418, 929
MalI GATC 6 cut(s) 126, 249, 291, 330, 540, 699
MboI GATC 6 cut(s) 124, 247, 289, 328, 538, 697
MboII GAAGA 3 cut(s) 326, 400, 617
MfeI CAATTG 3 cut(s) 24, 476, 639
MhlI GDGCHC 2 cut(s) 592, 663
MlyI GAGTC 2 cut(s) 339, 1104
MmeI TCCRAC 1 cut(s) 1192
Mph1103I ATGCAT 1 cut(s) 688
MroI TCCGGA 1 cut(s) 546
MseI TTAA 3 cut(s) 65, 339, 483
MspA1I CMGCKG 2 cut(s) 109, 1196
MspI CCGG 4 cut(s) 547, 807, 934, 1043
MspR9I CCNGG 2 cut(s) 87, 807
MunI CAATTG 3 cut(s) 24, 476, 639
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 6 cut(s) 277, 317, 716, 1003, 1190, 1193
NciI CCSGG 1 cut(s) 807
NdeI CATATG 2 cut(s) 835, 1162
NdeII GATC 6 cut(s) 124, 247, 289, 328, 538, 697
NlaIII CATG 7 cut(s) 375, 620, 755, 775, 1027, 1115, 1234
NlaIV GGNNCC 4 cut(s) 366, 502, 660, 1158
NmuCI GTSAC 1 cut(s) 418
NsiI ATGCAT 1 cut(s) 688
NspI RCATGY 3 cut(s) 620, 755, 1234
NspV TTCGAA 1 cut(s) 166
PaqCI CACCTGC 1 cut(s) 28
PciI ACATGT 1 cut(s) 751
PflMI CCANNNNNTGG 1 cut(s) 1166
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 6 cut(s) 47, 1012, 1183, 1186, 1195, 1198
PleI GAGTC 2 cut(s) 339, 1103
PpsI GAGTC 2 cut(s) 339, 1103
PscI ACATGT 1 cut(s) 751
Psp6I CCWGG 1 cut(s) 85
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 4 cut(s) 366, 502, 660, 1158
PspPI GGNCC 2 cut(s) 298, 821
PstI CTGCAG 2 cut(s) 970, 1195
PvuII CAGCTG 2 cut(s) 109, 1196
RsaI GTAC 6 cut(s) 467, 645, 831, 892, 897, 1244
RsaNI GTAC 6 cut(s) 466, 644, 830, 891, 896, 1243
SaqAI TTAA 3 cut(s) 65, 339, 483
SatI GCNGC 6 cut(s) 46, 1011, 1182, 1185, 1194, 1197
Sau3AI GATC 6 cut(s) 124, 247, 289, 328, 538, 697
Sau96I GGNCC 2 cut(s) 298, 821
ScaI AGTACT 1 cut(s) 897
SchI GAGTC 2 cut(s) 339, 1104
ScrFI CCNGG 2 cut(s) 87, 807
SduI GDGCHC 2 cut(s) 592, 663
SfaNI GCATC 2 cut(s) 57, 850
SfcI CTRYAG 2 cut(s) 966, 1191
SfuI TTCGAA 1 cut(s) 166
SinI GGWCC 1 cut(s) 298
SsiI CCGC 1 cut(s) 1204
SspI AATATT 2 cut(s) 459, 850
SspMI CTAG 2 cut(s) 630, 1246
StyD4I CCNGG 2 cut(s) 85, 805
StyI CCWWGG 1 cut(s) 629
TaaI ACNGT 4 cut(s) 658, 895, 1017, 1219
TaqI TCGA 3 cut(s) 166, 288, 1131
TaqII GACCGA 3 cut(s) 315, 885, 1134
TatI WGTACW 4 cut(s) 643, 890, 895, 1242
Tru1I TTAA 3 cut(s) 65, 339, 483
Tru9I TTAA 3 cut(s) 65, 339, 483
TscAI CASTG 5 cut(s) 327, 949, 975, 1022, 1195
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 6 cut(s) 45, 1010, 1181, 1184, 1193, 1196
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 1 cut(s) 360
TspGWI ACGGA 1 cut(s) 909
TspRI CASTG 5 cut(s) 327, 949, 975, 1022, 1195
Van91I CCANNNNNTGG 1 cut(s) 1166
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 4 cut(s) 233, 401, 727, 789
XceI RCATGY 3 cut(s) 620, 755, 1234
XcmI CCANNNNNNNNNTGG 1 cut(s) 1208
XmaJI CCTAGG 1 cut(s) 629
XmiI GTMKAC 1 cut(s) 635
XspI CTAG 2 cut(s) 630, 1246
ZrmI AGTACT 1 cut(s) 897
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.