MD03G1271700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012247
Physical Location & Seq
Reverse (-)
35483853 .. 35486033
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1271700.v1.1.491

Sequence Viewer

Length: 678 bp
ATGAACACCTTAACTTCAACGTCTCTCTGCAACCTCCAAGTTTCAAGGTATCCTTCCACCGTTTCGAACCAGAGACACGTTTCACCCTGCAATTCTCCACTTCCCGCCAAAATCTTCTTCAGACTTTATGCCAATGCCAATGAGCTGAATGCTCAGGCAGCAGTGGAGGAACCAAAGGAAGAGGTGGAAAAAGCCGAGCCACGCGAAGCCGGAAAATCCTCCACTTTCCCTGCTGCTGCTGCTTCTCCACTCGACAAGGACCTCAAAAAGGTTGTTCAGAAGACTGCTGCAACCTTTGCACCAAGGGCTTCCACAGCTTCCAAAAACCCTGCCGTGCCAGGCAGCACTTTGTACACTGTCTTCGAGGTTCAAGCGTACGCCTCCATGCTGCTAGGCGGAGTTCTGTCCTTCAATCTCATCTTTCCGTCAAATGAACCAGACATATGGAGATTAATGGGAATGTGGTCCATTTGGATGTTTACAATTCCTTCACTGCGCGCACGAGACTGCTCGAAAAATGAGAAGGAAGCTCTAAACTATCTCTTCCTCCTCGTCCCGTTACTCAATGTTACAATCCCATTCTTTATTAAGTCCTTTGCTGTCGTTTGGTCTGCTGATACCGTAGCCTTTTTCGCCATGTATGCGTGGAAGCTTGGTTGGCTGCAAACAAAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

24.98

Weight (kDa)

8.94

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012247)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G48070 AT2G48070 AT2G48070
fragaria_vesca FvH4_2g24170
malus_domestica MD03G1271700.v1.1 MD11G1292300.v1.1
prunus_persica Prupe.8G247900_v2.0.a1
pyrus_communis pycom03g21620
rosa_chinensis RchiOBHm_Chr6g0291981
rosa_laevigata RLG00000012073
rosa_multiflora Rmu_sc0026994.1_g000003 Rmu_sc0042914.1_g000001
rosa_roxburghii Rroxscaffold_7G00175520
rosa_rugosa Rorug06G0222200
rosa_samantha Rh6AG336000 Rh6BG343400 Rh6CG349600 Rh6DG336400
rosa_wichuraiana Rw6G029250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 204, 498
AciI CCGC 2 cut(s) 105, 396
AcuI CTGAAG 1 cut(s) 103
AfaI GTAC 2 cut(s) 353, 377
AfiI CCNNNNNNNGG 1 cut(s) 268
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 4 cut(s) 18, 45, 371, 412
AjnI CCWGG 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AluBI AGCT 4 cut(s) 145, 317, 530, 652
AluI AGCT 4 cut(s) 145, 317, 530, 652
Alw26I GTCTC 3 cut(s) 27, 67, 498
ApeKI GCWGC 8 cut(s) 158, 233, 236, 239, 287, 342, 388, 661
AseI ATTAAT 1 cut(s) 452
AspLEI GCGC 2 cut(s) 498, 500
AspS9I GGNCC 2 cut(s) 259, 465
AsuHPI GGTGA 1 cut(s) 75
AsuII TTCGAA 1 cut(s) 65
AvaII GGWCC 2 cut(s) 259, 465
BarI GAAGNNNNNNTAC 2 cut(s) 344, 376
BauI CACGAG 1 cut(s) 501
BbsI GAAGAC 2 cut(s) 287, 352
BbvI GCAGC 8 cut(s) 170, 220, 223, 226, 274, 354, 375, 648
BceAI ACGGC 1 cut(s) 317
BciT130I CCWGG 1 cut(s) 339
BciVI GTATCC 1 cut(s) 60
BcoDI GTCTC 3 cut(s) 27, 67, 498
BfaI CTAG 2 cut(s) 392, 676
BfuI GTATCC 1 cut(s) 60
BisI GCNGC 8 cut(s) 159, 234, 237, 240, 288, 343, 389, 662
BlsI GCNGC 8 cut(s) 160, 235, 238, 241, 289, 344, 390, 663
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 2 cut(s) 259, 465
BmgT120I GGNCC 2 cut(s) 259, 465
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 1 cut(s) 339
BpiI GAAGAC 2 cut(s) 287, 352
Bpu10I CCTNAGC 1 cut(s) 153
Bpu14I TTCGAA 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 302
Bsc4I CCNNNNNNNGG 1 cut(s) 268
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 302
BseGI GGATG 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMII CTCAG 1 cut(s) 167
BsePI GCGCGC 1 cut(s) 496
BseRI GAGGAG 1 cut(s) 539
BseXI GCAGC 8 cut(s) 170, 220, 223, 226, 274, 354, 375, 648
Bsh1236I CGCG 2 cut(s) 204, 498
BsiSI CCGG 1 cut(s) 210
BsiWI CGTACG 1 cut(s) 375
BslFI GGGAC 1 cut(s) 539
BslI CCNNNNNNNGG 1 cut(s) 268
BsmAI GTCTC 3 cut(s) 27, 67, 498
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 1 cut(s) 539
BsmI GAATGC 1 cut(s) 154
Bsp119I TTCGAA 1 cut(s) 65
Bsp1407I TGTACA 1 cut(s) 351
BspACI CCGC 2 cut(s) 105, 396
BspCNI CTCAG 1 cut(s) 166
BspFNI CGCG 2 cut(s) 204, 498
BspLI GGNNCC 1 cut(s) 171
BspT104I TTCGAA 1 cut(s) 65
BsrGI TGTACA 1 cut(s) 351
BssECI CCNNGG 1 cut(s) 302
BssHII GCGCGC 1 cut(s) 496
BssSI CACGAG 1 cut(s) 501
BssT1I CCWWGG 1 cut(s) 302
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 3 cut(s) 61, 358, 622
Bst6I CTCTTC 2 cut(s) 174, 548
BstAPI GCANNNNNTGC 1 cut(s) 296
BstAUI TGTACA 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 1 cut(s) 153
BstENI CCTNNNNNAGG 1 cut(s) 266
BstF5I GGATG 1 cut(s) 480
BstFNI CGCG 2 cut(s) 204, 498
BstHHI GCGC 2 cut(s) 498, 500
BstMAI GTCTC 3 cut(s) 27, 67, 498
BstMWI GCNNNNNNNGC 8 cut(s) 158, 239, 296, 305, 314, 632, 641, 658
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstUI CGCG 2 cut(s) 204, 498
BstV1I GCAGC 8 cut(s) 170, 220, 223, 226, 274, 354, 375, 648
BstV2I GAAGAC 2 cut(s) 287, 352
BstXI CCANNNNNNTGG 1 cut(s) 444
BsuI GTATCC 1 cut(s) 60
BtsCI GGATG 1 cut(s) 480
BtsI GCAGTG 2 cut(s) 168, 491
BtsIMutI CAGTG 3 cut(s) 168, 354, 491
Cac8I GCNNGC 1 cut(s) 498
CfoI GCGC 2 cut(s) 498, 500
Cfr13I GGNCC 2 cut(s) 259, 465
Csp6I GTAC 2 cut(s) 352, 376
CviAII CATG 2 cut(s) 385, 637
CviQI GTAC 2 cut(s) 352, 376
DdeI CTNAG 1 cut(s) 153
Eam1104I CTCTTC 2 cut(s) 174, 548
EarI CTCTTC 2 cut(s) 174, 548
EciI GGCGGA 1 cut(s) 411
Eco130I CCWWGG 1 cut(s) 302
Eco47I GGWCC 2 cut(s) 259, 465
Eco57I CTGAAG 1 cut(s) 103
EcoNI CCTNNNNNAGG 1 cut(s) 266
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 302
ErhI CCWWGG 1 cut(s) 302
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 2 cut(s) 388, 640
FaiI YATR 6 cut(s) 129, 386, 443, 445, 638, 642
FalI AAGNNNNNCTT 2 cut(s) 37, 69
FaqI GGGAC 1 cut(s) 539
FatI CATG 2 cut(s) 384, 636
FauI CCCGC 1 cut(s) 112
FauNDI CATATG 1 cut(s) 443
Fnu4HI GCNGC 8 cut(s) 159, 234, 237, 240, 288, 343, 389, 662
FokI GGATG 1 cut(s) 487
Fsp4HI GCNGC 8 cut(s) 159, 234, 237, 240, 288, 343, 389, 662
FspBI CTAG 2 cut(s) 392, 676
GlaI GCGC 2 cut(s) 497, 499
GluI GCNGC 8 cut(s) 159, 234, 237, 240, 288, 343, 389, 662
HapII CCGG 1 cut(s) 210
HhaI GCGC 2 cut(s) 498, 500
Hin1II CATG 2 cut(s) 388, 640
Hin6I GCGC 2 cut(s) 496, 498
HinP1I GCGC 2 cut(s) 496, 498
HindIII AAGCTT 1 cut(s) 650
HpaII CCGG 1 cut(s) 210
HphI GGTGA 1 cut(s) 75
Hpy166II GTNNAC 2 cut(s) 354, 480
Hpy188I TCNGA 2 cut(s) 122, 279
Hpy8I GTNNAC 2 cut(s) 354, 480
HpyAV CCTTC 4 cut(s) 63, 418, 498, 517
HpyCH4III ACNGT 3 cut(s) 61, 358, 622
HpyCH4IV ACGT 2 cut(s) 20, 78
HpyCH4V TGCA 5 cut(s) 30, 90, 290, 299, 664
HpyF10VI GCNNNNNNNGC 8 cut(s) 158, 239, 296, 305, 314, 632, 641, 658
HpyF3I CTNAG 1 cut(s) 153
HpySE526I ACGT 2 cut(s) 20, 78
Hsp92II CATG 2 cut(s) 388, 640
HspAI GCGC 2 cut(s) 496, 498
LpnPI CCDG 9 cut(s) 83, 100, 140, 223, 243, 324, 342, 351, 450
Lsp1109I GCAGC 8 cut(s) 170, 220, 223, 226, 274, 354, 375, 648
MaeI CTAG 2 cut(s) 392, 676
MaeII ACGT 2 cut(s) 20, 78
MaeIII GTNAC 2 cut(s) 558, 568
MboII GAAGA 6 cut(s) 106, 109, 191, 292, 352, 535
MluCI AATT 2 cut(s) 91, 483
MnlI CCTC 9 cut(s) 44, 160, 175, 229, 272, 358, 391, 557, 560
MseI TTAA 3 cut(s) 11, 452, 588
MslI CAYNNNNRTG 1 cut(s) 473
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 154
MvaI CCWGG 1 cut(s) 339
MvnI CGCG 2 cut(s) 204, 498
MwoI GCNNNNNNNGC 8 cut(s) 158, 239, 296, 305, 314, 632, 641, 658
NdeI CATATG 1 cut(s) 443
NlaIII CATG 2 cut(s) 388, 640
NlaIV GGNNCC 1 cut(s) 171
NmeAIII GCCGAG 1 cut(s) 220
NspV TTCGAA 1 cut(s) 65
PauI GCGCGC 1 cut(s) 496
PctI GAATGC 1 cut(s) 154
Pfl23II CGTACG 1 cut(s) 375
PkrI GCNGC 8 cut(s) 160, 235, 238, 241, 289, 344, 390, 663
PpuMI RGGWCCY 1 cut(s) 259
PshBI ATTAAT 1 cut(s) 452
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspLI CGTACG 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 171
PspPI GGNCC 2 cut(s) 259, 465
PspPPI RGGWCCY 1 cut(s) 259
PteI GCGCGC 1 cut(s) 496
RsaI GTAC 2 cut(s) 353, 377
RsaNI GTAC 2 cut(s) 352, 376
RseI CAYNNNNRTG 1 cut(s) 473
SaqAI TTAA 3 cut(s) 11, 452, 588
SatI GCNGC 8 cut(s) 159, 234, 237, 240, 288, 343, 389, 662
Sau96I GGNCC 2 cut(s) 259, 465
ScrFI CCNGG 1 cut(s) 339
SfuI TTCGAA 1 cut(s) 65
SinI GGWCC 2 cut(s) 259, 465
SmiMI CAYNNNNRTG 1 cut(s) 473
Sse9I AATT 2 cut(s) 91, 483
SsiI CCGC 2 cut(s) 105, 396
SspMI CTAG 2 cut(s) 392, 676
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 1 cut(s) 302
TaaI ACNGT 3 cut(s) 61, 358, 622
TaiI ACGT 2 cut(s) 23, 81
TaqI TCGA 4 cut(s) 65, 252, 363, 512
TasI AATT 2 cut(s) 91, 483
TatI WGTACW 1 cut(s) 351
Tru1I TTAA 3 cut(s) 11, 452, 588
Tru9I TTAA 3 cut(s) 11, 452, 588
TscAI CASTG 3 cut(s) 168, 361, 498
TseI GCWGC 8 cut(s) 158, 233, 236, 239, 287, 342, 388, 661
TspDTI ATGAA 2 cut(s) 17, 447
TspGWI ACGGA 1 cut(s) 414
TspRI CASTG 3 cut(s) 168, 361, 498
VpaK11BI GGWCC 2 cut(s) 259, 465
VspI ATTAAT 1 cut(s) 452
XagI CCTNNNNNAGG 1 cut(s) 266
XspI CTAG 2 cut(s) 392, 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.