Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003338

Physical Location & Seq

Genomic Coordinates Reverse (-)

36931484 .. 36937325

Gene Structure 5842 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1292300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1488 bp

ATGCAAATGGCCATGTACATCGTATCACGGCGGGCAGCCGGAGAGTTGACGCCGTTGGCGGCGTTCCGCTACGCTACATCTTCGAGAGCCTATTCGACGTCGTTTAGAGAGGAAAGGGACACTTTTGGACCCATCAACGTTCCCTCAGACAAGCTGTGGGGGGCGCAGACTCAGAGATCGCTGCAGAATTTCGACATCGGCGGCGAGCGGGAGCGAATGCCCGAACCCATTGTTCGTGCTTTTGGGGTGCTTAAAAAATGCGCTGCAAAGGTGAACATGGAGTATGGGCTTGATCCAACTATTGGGAAAGCAATAATGCAAGCTGCCCAAGAAGTCGCCGAGGGAAAGCTCAACCAACACTTTCCTCTTGTTATCTGGCAAACTGGCAGTGGAACCCAAAGCAACATGAATGTCAATGAGGTCATTGCGAACAGGGCAGCTGAGATACTTGGCCATCAGCGCGGCGAGAAGTTTGTGCACCCGAATGACCATGTTAATAGATCACAATCTTCCAATGACACATTCCCAACTGTCATGCACATTGCATCTGCAAAGGAAATTAACTCAAGACTGATACCAAATTTGCAAACTTTGCATGCTGCCCTACATTCAAAGTCTGAAGAATTCAAAGATATTGTTAAAATTGGACGAACCCACACGCAAGATGCGACACCTTTGACCCTTGGGCAAGAGTTCAGCGGCTATTCAACACAAGTTAAGTATGGAATTGAACGAGTTTTGCATACTCTACCTCGTATGTATCAGCTTGCACAGGGTGGTACTGCTGTTGGGACTGGATTGAACACAAAGAAGGGATTTGATGTAAAGATAGCTGCAGCAGTAGCTGAGGAAACAAAGTTACCATTTGTCACTGCAGAAAACAAGTTTGAAGCTTTGGCTGCACATGATGCTGCTGTTGAAACTAGCGGAGCCCTAAACACAGTTGCTACCTCTTTGATGAAGATTGCAAATGACATACGTTTCTTAGGAAGTGGTCCACGTTGTGGCCTTGGTGAACTCATTCTCCCTGAAAACGAGCCTGGTAGCAGCATTATGCCTGGGAAGGTAAACCCTACCCAGTGTGAGGCTCTCACAATGGTCTGTGCTCGGGTTATGGGTAACAATGTTGCCATTACAGTAGGTGGATCAAATGGTCACTTTGAACTTAATGTGTTCAAGCCAATGATTGCTAGTGGTCTCCTACAATCTCTGAGATTGCTTGGGGATGCATCTGCTTCCTTTGAAAAGAACTGCGTGAGAGGAATTCAAGCTAATAGGGAAAGAATTTCAAAGTTTCTTCATGAGTCACTTATGTTGGTCACATCCTTGAATCATAAAATTGGTTATGATAATGCCGCGGCAGTTGCAAAGACAGCCCACAAGGAGGGATCCACTTTGAAGGAAGCTGCATTGAAACTTCGAATGCTATCAAGCGAAGAATTTGATACTATTGTGGTGCCAGAGAAGATGATTGGCCCAACTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004333 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006106 GO:0006108 GO:0006807 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0008152 GO:0009060 GO:0009266 GO:0009409 GO:0009628 GO:0009631 GO:0009651 GO:0009856 GO:0009987 GO:0010109 GO:0015980 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017144 GO:0019222 GO:0019752 GO:0022414 GO:0031323 GO:0032501 GO:0032502 GO:0042126 GO:0042128 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0045333 GO:0048856 GO:0048868 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0055114 GO:0065007 GO:0071704 GO:0071941 GO:0072350 GO:2001057

KEGG Pathways

Metabolic & Signaling

ko00020 ko00620 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko04934 ko05200 ko05211 map00020 map00620 map00720 map01100 map01110 map01120 map01130 map01200 map04934 map05200 map05211

Pfam Domains

Protein Families

FumaraseC_C Lyase_1

Protein Analysis

496

Amino Acids

53.78

Weight (kDa)

8.45

Isoelectric Point (pI)

30.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lyase_1	PF00206	43 - 373	1.3e-117	Lyase
FumaraseC_C	PF10415	439 - 491	9.2e-22	Fumarase C C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003338)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47510 AT2G47510 AT2G47510 AT5G50950 AT5G50950 AT5G50950 AT5G50950
fragaria_vesca	FvH4_2g26121 FvH4_2g26121 FvH4_2g26140 FvH4_2g26140
malus_domestica	MD00G1140200.v1.1 MD03G1292300.v1.1
prunus_persica	Prupe.8G265300_v2.0.a1 Prupe.8G265300_v2.0.a1 Prupe.8G265300_v2.0.a1
pyrus_communis	pycom03g23150 pycom11g27280
rosa_chinensis	RchiOBHm_Chr6g0294511 RchiOBHm_Chr6g0294521
rosa_laevigata	RLG00000011863
rosa_multiflora	Rmu_ssc0000248.1_g000007
rosa_roxburghii	Rroxscaffold_7G00173260 Rroxscaffold_7G00173270
rosa_rugosa	Rorug06G0245500 Rorug06G0245500
rosa_samantha	Rh6AG357800 Rh6AG357900 Rh6BG364700 Rh6BG364800 Rh6CG371800 Rh6CG371900 Rh6DG358600 Rh6DG358700
rosa_wichuraiana	Rw6G031210 Rw6G031220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	101
AccB1I	GGYRCC	1 cut(s)	1456
AccB7I	CCANNNNNTGG	2 cut(s)	302, 1004
AccBSI	CCGCTC	1 cut(s)	208
AccII	CGCG	2 cut(s)	462, 1358
AclI	AACGTT	1 cut(s)	138
AclWI	GGATC	4 cut(s)	287, 1153, 1383, 1396
AcoI	YGGCCR	2 cut(s)	9, 451
AcsI	RAATTY	6 cut(s)	187, 580, 623, 1263, 1284, 1439
AcuI	CTGAAG	1 cut(s)	639
AcyI	GRCGYC	2 cut(s)	50, 98
AdeI	CACNNNGTG	2 cut(s)	776, 1004
AfaI	GTAC	2 cut(s)	17, 781
AfiI	CCNNNNNNNGG	4 cut(s)	302, 1004, 1084, 1384
AjnI	CCWGG	2 cut(s)	1039, 1057
AjuI	GAANNNNNNNTTGG	2 cut(s)	506, 538
AloI	GAACNNNNNNTCC	2 cut(s)	1008, 1040
Alw21I	GWGCWC	2 cut(s)	480, 1108
Alw26I	GTCTC	1 cut(s)	1202
Alw44I	GTGCAC	1 cut(s)	476
AlwI	GGATC	4 cut(s)	287, 1153, 1383, 1396
AlwNI	CAGNNNCTG	1 cut(s)	845
Ama87I	CYCGRG	1 cut(s)	1107
AoxI	GGCC	4 cut(s)	9, 451, 1006, 1474
ApaLI	GTGCAC	1 cut(s)	476
ApoI	RAATTY	6 cut(s)	187, 580, 623, 1263, 1284, 1439
Asp700I	GAANNNNTTC	1 cut(s)	1020
AspLEI	GCGC	3 cut(s)	166, 263, 462
AspS9I	GGNCC	3 cut(s)	128, 995, 1475
AsuHPI	GGTGA	2 cut(s)	283, 1025
AsuII	TTCGAA	1 cut(s)	1420
AvaI	CYCGRG	1 cut(s)	1107
AvaII	GGWCC	2 cut(s)	128, 995
BaeGI	GKGCMC	1 cut(s)	480
BaeI	ACNNNNGTAYC	4 cut(s)	771, 804, 1437, 1470
BalI	TGGCCA	2 cut(s)	11, 453
BamHI	GGATCC	1 cut(s)	1388
BanI	GGYRCC	1 cut(s)	1456
BanII	GRGCYC	1 cut(s)	934
Bbv12I	GWGCWC	2 cut(s)	480, 1108
BbvCI	CCTCAGC	1 cut(s)	846
BccI	CCATC	2 cut(s)	140, 462
BceAI	ACGGC	2 cut(s)	37, 44
BciT130I	CCWGG	2 cut(s)	1041, 1059
BcoDI	GTCTC	1 cut(s)	1202
BfaI	CTAG	2 cut(s)	924, 1191
BfmI	CTRYAG	3 cut(s)	182, 834, 873
Bme1390I	CCNGG	2 cut(s)	1041, 1059
Bme18I	GGWCC	2 cut(s)	128, 995
BmeT110I	CYCGRG	1 cut(s)	1107
BmgT120I	GGNCC	3 cut(s)	128, 995, 1475
BmiI	GGNNCC	5 cut(s)	130, 394, 931, 1390, 1458
BmrFI	CCNGG	2 cut(s)	1041, 1059
BmrI	ACTGGG	1 cut(s)	1072
BmsI	GCATC	5 cut(s)	554, 655, 898, 1216, 1238
BmuI	ACTGGG	1 cut(s)	1072
Bpu10I	CCTNAGC	1 cut(s)	846
Bpu14I	TTCGAA	1 cut(s)	1420
BpuEI	CTTGAG	1 cut(s)	550
BsaBI	GATNNNNATC	1 cut(s)	505
BsaHI	GRCGYC	2 cut(s)	50, 98
BsaI	GGTCTC	1 cut(s)	1202
BsaJI	CCNNGG	5 cut(s)	339, 682, 1009, 1058, 1356
BsaXI	ACNNNNNCTCC	2 cut(s)	1008, 1038
Bsc4I	CCNNNNNNNGG	4 cut(s)	302, 1004, 1084, 1384
Bse1I	ACTGG	3 cut(s)	388, 799, 1078
Bse3DI	GCAATG	2 cut(s)	423, 540
Bse8I	GATNNNNATC	1 cut(s)	505
BseBI	CCWGG	2 cut(s)	1041, 1059
BseDI	CCNNGG	5 cut(s)	339, 682, 1009, 1058, 1356
BseGI	GGATG	2 cut(s)	1231, 1322
BseJI	GATNNNNATC	1 cut(s)	505
BseLI	CCNNNNNNNGG	4 cut(s)	302, 1004, 1084, 1384
BseMI	GCAATG	2 cut(s)	423, 540
BseMII	CTCAG	5 cut(s)	159, 185, 432, 837, 1202
BseNI	ACTGG	3 cut(s)	388, 799, 1078
BseSI	GKGCMC	1 cut(s)	480
BsgI	GTGCAG	1 cut(s)	885
Bsh1236I	CGCG	2 cut(s)	462, 1358
BshFI	GGCC	4 cut(s)	11, 453, 1008, 1476
BshNI	GGYRCC	1 cut(s)	1456
BsiHKAI	GWGCWC	2 cut(s)	480, 1108
BsiHKCI	CYCGRG	1 cut(s)	1107
BsiSI	CCGG	1 cut(s)	39
BslFI	GGGAC	2 cut(s)	131, 805
BslI	CCNNNNNNNGG	4 cut(s)	302, 1004, 1084, 1384
BsmAI	GTCTC	1 cut(s)	1202
BsmFI	GGGAC	2 cut(s)	131, 805
BsmI	GAATGC	2 cut(s)	222, 1428
BsnI	GGCC	4 cut(s)	11, 453, 1008, 1476
Bso31I	GGTCTC	1 cut(s)	1202
BsoBI	CYCGRG	1 cut(s)	1107
Bsp119I	TTCGAA	1 cut(s)	1420
Bsp1286I	GDGCHC	3 cut(s)	480, 934, 1108
Bsp1407I	TGTACA	1 cut(s)	15
Bsp143I	GATC	5 cut(s)	176, 292, 500, 1145, 1388
BspANI	GGCC	4 cut(s)	11, 453, 1008, 1476
BspCNI	CTCAG	5 cut(s)	158, 184, 433, 838, 1203
BspFNI	CGCG	2 cut(s)	462, 1358
BspHI	TCATGA	1 cut(s)	1300
BspLI	GGNNCC	5 cut(s)	130, 394, 931, 1390, 1458
BspMAI	CTGCAG	3 cut(s)	186, 838, 877
BspPI	GGATC	4 cut(s)	287, 1153, 1383, 1396
BspT104I	TTCGAA	1 cut(s)	1420
BspT107I	GGYRCC	1 cut(s)	1456
BspTNI	GGTCTC	1 cut(s)	1202
BsrBI	CCGCTC	1 cut(s)	208
BsrDI	GCAATG	2 cut(s)	423, 540
BsrGI	TGTACA	1 cut(s)	15
BsrI	ACTGG	3 cut(s)	388, 799, 1078
BssECI	CCNNGG	5 cut(s)	339, 682, 1009, 1058, 1356
BssMI	GATC	5 cut(s)	176, 292, 500, 1145, 1388
BssNI	GRCGYC	2 cut(s)	50, 98
BssT1I	CCWWGG	2 cut(s)	682, 1009
Bst2UI	CCWGG	2 cut(s)	1041, 1059
Bst4CI	ACNGT	3 cut(s)	532, 943, 1138
BstACI	GRCGYC	2 cut(s)	50, 98
BstAPI	GCANNNNNTGC	2 cut(s)	592, 908
BstAUI	TGTACA	1 cut(s)	15
BstBI	TTCGAA	1 cut(s)	1420
BstC8I	GCNNGC	5 cut(s)	33, 206, 321, 597, 768
BstDEI	CTNAG	6 cut(s)	145, 171, 441, 846, 985, 1211
BstDSI	CCRYGG	1 cut(s)	1356
BstF5I	GGATG	2 cut(s)	1231, 1322
BstFNI	CGCG	2 cut(s)	462, 1358
BstHHI	GCGC	3 cut(s)	166, 263, 462
BstKTI	GATC	5 cut(s)	179, 295, 503, 1148, 1391
BstMAI	GTCTC	1 cut(s)	1202
BstMBI	GATC	5 cut(s)	176, 292, 500, 1145, 1388
BstMWI	GCNNNNNNNGC	8 cut(s)	434, 459, 592, 842, 899, 908, 1364, 1373
BstNI	CCWGG	2 cut(s)	1041, 1059
BstNSI	RCATGY	1 cut(s)	599
BstSCI	CCNGG	2 cut(s)	1039, 1057
BstSFI	CTRYAG	3 cut(s)	182, 834, 873
BstSLI	GKGCMC	1 cut(s)	480
BstUI	CGCG	2 cut(s)	462, 1358
BstX2I	RGATCY	1 cut(s)	1388
BstYI	RGATCY	1 cut(s)	1388
BsuRI	GGCC	4 cut(s)	11, 453, 1008, 1476
BtgI	CCRYGG	1 cut(s)	1356
BtsCI	GGATG	2 cut(s)	1231, 1322
BtsI	GCAGTG	2 cut(s)	394, 870
BtsIMutI	CAGTG	3 cut(s)	394, 870, 1085
Cac8I	GCNNGC	5 cut(s)	33, 206, 321, 597, 768
CaiI	CAGNNNCTG	1 cut(s)	845
CciI	TCATGA	1 cut(s)	1300
CfoI	GCGC	3 cut(s)	166, 263, 462
Cfr13I	GGNCC	3 cut(s)	128, 995, 1475
Cfr42I	CCGCGG	1 cut(s)	1359
CseI	GACGC	1 cut(s)	58
Csp6I	GTAC	2 cut(s)	16, 780
CviAII	CATG	8 cut(s)	13, 277, 406, 491, 535, 596, 905, 1301
CviQI	GTAC	2 cut(s)	16, 780
DdeI	CTNAG	6 cut(s)	145, 171, 441, 846, 985, 1211
DpnI	GATC	5 cut(s)	178, 294, 502, 1147, 1390
DpnII	GATC	5 cut(s)	176, 292, 500, 1145, 1388
DraIII	CACNNNGTG	2 cut(s)	776, 1004
EaeI	YGGCCR	2 cut(s)	9, 451
Eco130I	CCWWGG	2 cut(s)	682, 1009
Eco24I	GRGCYC	1 cut(s)	934
Eco31I	GGTCTC	1 cut(s)	1202
Eco47I	GGWCC	2 cut(s)	128, 995
Eco57I	CTGAAG	1 cut(s)	639
Eco88I	CYCGRG	1 cut(s)	1107
EcoRI	GAATTC	2 cut(s)	623, 1263
EcoRII	CCWGG	2 cut(s)	1039, 1057
EcoT14I	CCWWGG	2 cut(s)	682, 1009
EcoT22I	ATGCAT	1 cut(s)	1231
EcoT38I	GRGCYC	1 cut(s)	934
ErhI	CCWWGG	2 cut(s)	682, 1009
FaeI	CATG	8 cut(s)	16, 280, 409, 494, 538, 599, 908, 1304
FalI	AAGNNNNNCTT	2 cut(s)	106, 138
FaqI	GGGAC	2 cut(s)	131, 805
FatI	CATG	8 cut(s)	12, 276, 405, 490, 534, 595, 904, 1300
FauI	CCCGC	2 cut(s)	24, 201
FokI	GGATG	2 cut(s)	1238, 1309
FriOI	GRGCYC	1 cut(s)	934
FspBI	CTAG	2 cut(s)	924, 1191
GlaI	GCGC	3 cut(s)	165, 262, 461
HaeIII	GGCC	4 cut(s)	11, 453, 1008, 1476
HapII	CCGG	1 cut(s)	39
HgaI	GACGC	1 cut(s)	58
HhaI	GCGC	3 cut(s)	166, 263, 462
Hin1I	GRCGYC	2 cut(s)	50, 98
Hin1II	CATG	8 cut(s)	16, 280, 409, 494, 538, 599, 908, 1304
Hin6I	GCGC	3 cut(s)	164, 261, 460
HinP1I	GCGC	3 cut(s)	164, 261, 460
HincII	GTYRAC	1 cut(s)	48
HindII	GTYRAC	1 cut(s)	48
HindIII	AAGCTT	1 cut(s)	891
HinfI	GANTC	3 cut(s)	169, 1304, 1330
HpaII	CCGG	1 cut(s)	39
HphI	GGTGA	2 cut(s)	283, 1025
Hpy166II	GTNNAC	6 cut(s)	48, 274, 478, 998, 1016, 1069
Hpy188I	TCNGA	4 cut(s)	148, 174, 619, 1212
Hpy188III	TCNNGA	3 cut(s)	84, 567, 1301
Hpy8I	GTNNAC	6 cut(s)	48, 274, 478, 998, 1016, 1069
Hpy99I	CGWCG	2 cut(s)	100, 103
HpyAV	CCTTC	3 cut(s)	805, 1057, 1393
HpyCH4III	ACNGT	3 cut(s)	532, 943, 1138
HpyCH4IV	ACGT	4 cut(s)	98, 138, 979, 1000
HpyF10VI	GCNNNNNNNGC	8 cut(s)	434, 459, 592, 842, 899, 908, 1364, 1373
HpyF3I	CTNAG	6 cut(s)	145, 171, 441, 846, 985, 1211
HpySE526I	ACGT	4 cut(s)	98, 138, 979, 1000
Hsp92I	GRCGYC	2 cut(s)	50, 98
Hsp92II	CATG	8 cut(s)	16, 280, 409, 494, 538, 599, 908, 1304
HspAI	GCGC	3 cut(s)	164, 261, 460
KspI	CCGCGG	1 cut(s)	1359
Kzo9I	GATC	5 cut(s)	176, 292, 500, 1145, 1388
LmnI	GCTCC	2 cut(s)	211, 929
LweI	GCATC	5 cut(s)	554, 655, 898, 1216, 1238
MaeI	CTAG	2 cut(s)	924, 1191
MaeII	ACGT	4 cut(s)	98, 138, 979, 1000
MaeIII	GTNAC	6 cut(s)	858, 868, 1118, 1154, 1305, 1318
MalI	GATC	5 cut(s)	178, 294, 502, 1147, 1390
MbiI	CCGCTC	1 cut(s)	208
MboI	GATC	5 cut(s)	176, 292, 500, 1145, 1388
MboII	GAAGA	7 cut(s)	72, 501, 632, 973, 1289, 1448, 1477
MflI	RGATCY	1 cut(s)	1388
MhlI	GDGCHC	3 cut(s)	480, 934, 1108
MlsI	TGGCCA	2 cut(s)	11, 453
MluNI	TGGCCA	2 cut(s)	11, 453
MlyI	GAGTC	2 cut(s)	163, 1313
MmeI	TCCRAC	1 cut(s)	320
Mox20I	TGGCCA	2 cut(s)	11, 453
Mph1103I	ATGCAT	1 cut(s)	1231
MroXI	GAANNNNTTC	1 cut(s)	1020
MscI	TGGCCA	2 cut(s)	11, 453
MseI	TTAA	6 cut(s)	252, 495, 561, 639, 717, 1167
MslI	CAYNNNNRTG	1 cut(s)	483
Msp20I	TGGCCA	2 cut(s)	11, 453
MspA1I	CMGCKG	3 cut(s)	440, 699, 1358
MspI	CCGG	1 cut(s)	39
MspR9I	CCNGG	2 cut(s)	1041, 1059
Mva1269I	GAATGC	2 cut(s)	222, 1428
MvaI	CCWGG	2 cut(s)	1041, 1059
MvnI	CGCG	2 cut(s)	462, 1358
MwoI	GCNNNNNNNGC	8 cut(s)	434, 459, 592, 842, 899, 908, 1364, 1373
NdeII	GATC	5 cut(s)	176, 292, 500, 1145, 1388
NlaIII	CATG	8 cut(s)	16, 280, 409, 494, 538, 599, 908, 1304
NlaIV	GGNNCC	5 cut(s)	130, 394, 931, 1390, 1458
NmeAIII	GCCGAG	1 cut(s)	364
NmuCI	GTSAC	4 cut(s)	868, 1154, 1305, 1318
NsiI	ATGCAT	1 cut(s)	1231
NspI	RCATGY	1 cut(s)	599
NspV	TTCGAA	1 cut(s)	1420
PaeI	GCATGC	1 cut(s)	599
PagI	TCATGA	1 cut(s)	1300
PcsI	WCGNNNNNNNCGW	1 cut(s)	665
PctI	GAATGC	2 cut(s)	222, 1428
PdmI	GAANNNNTTC	1 cut(s)	1020
PfeI	GAWTC	1 cut(s)	1330
PflMI	CCANNNNNTGG	2 cut(s)	302, 1004
PleI	GAGTC	2 cut(s)	163, 1312
PpsI	GAGTC	2 cut(s)	163, 1312
Psp1406I	AACGTT	1 cut(s)	138
Psp6I	CCWGG	2 cut(s)	1039, 1057
PspGI	CCWGG	2 cut(s)	1039, 1057
PspN4I	GGNNCC	5 cut(s)	130, 394, 931, 1390, 1458
PspPI	GGNCC	3 cut(s)	128, 995, 1475
PstI	CTGCAG	3 cut(s)	186, 838, 877
PstNI	CAGNNNCTG	1 cut(s)	845
PsuI	RGATCY	1 cut(s)	1388
PvuII	CAGCTG	1 cut(s)	440
RsaI	GTAC	2 cut(s)	17, 781
RsaNI	GTAC	2 cut(s)	16, 780
RseI	CAYNNNNRTG	1 cut(s)	483
SacII	CCGCGG	1 cut(s)	1359
SaqAI	TTAA	6 cut(s)	252, 495, 561, 639, 717, 1167
Sau3AI	GATC	5 cut(s)	176, 292, 500, 1145, 1388
Sau96I	GGNCC	3 cut(s)	128, 995, 1475
SchI	GAGTC	2 cut(s)	163, 1313
ScrFI	CCNGG	2 cut(s)	1041, 1059
SduI	GDGCHC	3 cut(s)	480, 934, 1108
SfaNI	GCATC	5 cut(s)	554, 655, 898, 1216, 1238
SfcI	CTRYAG	3 cut(s)	182, 834, 873
Sfr303I	CCGCGG	1 cut(s)	1359
SfuI	TTCGAA	1 cut(s)	1420
SgrBI	CCGCGG	1 cut(s)	1359
SinI	GGWCC	2 cut(s)	128, 995
SmiMI	CAYNNNNRTG	1 cut(s)	483
SmlI	CTYRAG	1 cut(s)	565
SmoI	CTYRAG	1 cut(s)	565
SphI	GCATGC	1 cut(s)	599
SspMI	CTAG	2 cut(s)	924, 1191
StyD4I	CCNGG	2 cut(s)	1039, 1057
StyI	CCWWGG	2 cut(s)	682, 1009
TaaI	ACNGT	3 cut(s)	532, 943, 1138
TaiI	ACGT	4 cut(s)	101, 141, 982, 1003
TaqI	TCGA	4 cut(s)	83, 95, 192, 1420
TatI	WGTACW	1 cut(s)	15
TauI	GCSGC	6 cut(s)	62, 204, 465, 702, 1358, 1361
TfiI	GAWTC	1 cut(s)	1330
Tru1I	TTAA	6 cut(s)	252, 495, 561, 639, 717, 1167
Tru9I	TTAA	6 cut(s)	252, 495, 561, 639, 717, 1167
TscAI	CASTG	3 cut(s)	394, 877, 1085
TseFI	GTSAC	4 cut(s)	868, 1154, 1305, 1318
Tsp45I	GTSAC	4 cut(s)	868, 1154, 1305, 1318
TspDTI	ATGAA	3 cut(s)	422, 974, 1289
TspRI	CASTG	3 cut(s)	394, 877, 1085
Van91I	CCANNNNNTGG	2 cut(s)	302, 1004
VneI	GTGCAC	1 cut(s)	476
VpaK11BI	GGWCC	2 cut(s)	128, 995
XapI	RAATTY	6 cut(s)	187, 580, 623, 1263, 1284, 1439
XceI	RCATGY	1 cut(s)	599
XmnI	GAANNNNTTC	1 cut(s)	1020
XspI	CTAG	2 cut(s)	924, 1191
ZraI	GACGTC	1 cut(s)	99
Zsp2I	ATGCAT	1 cut(s)	1231

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.