Rorug06G0245500

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003338
Physical Location & Seq
Reverse (-)
39175215 .. 39181423
6209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0245500.1

Sequence Viewer

Length: 1464 bp
ATGAAGATCACACCATTTCTCGATCTCACTACTCGGTTGGCCTATACAGCTCCTACATGGGCAAGTCTAAGTTGTGGCATCTTTGTACTCATCACTCTCACGCTTTCAATCTATCTTCTCTTTGAGCACCTTTCCGCATACAAGAATCCTGAGGAACAAAAGTTTTTAATTGGTGTCATCCTCATGGTTCCATGCTATGCAGTAGAGTCATTAGTGTCTTTGGTGAACCCATCAATAAGTGTTGATTGCGCAATTCTACGGGATTGTTACGAATCTTTTGCCATGTATTGCTTTGGAAGATACCTGGTTGCATGTTTAGGTGGGGAAGAAAGGACAATCGAGTTTATGGAAAGACAAGGACGAGCAAGTTATAAGACTCCCCTATTAGTAGACAGTTCCGGAAATGGAACTGTCACGCATCCTTTTCCAATGAACTTAATCTTGAAACCATGGAAACTTGGTCAAGGATTTTACCAACTTGTCAAATTCGGAATCGTTCAGTATATGATATTCAAGTCTCTCAGTGCTATTCTAGCAGTTATCCTTGAAGCTTTTGGTGTATACTGTGATGGAGAATTTAAATGGGGATGTGGGTATCCTTATATTGCAGTGATTCTAAATTTTAGTCAGTCATGGGCTTTGTACTGTCTAGTTCAGTTTTACGCCGTTACAAAAGATGAACTAGCTCATATAAAGCCATTATACAAGTTTCTGACTTTTAAGTCGATTGTGTTCTTGACATGGTGGCAAGGTGTGGCAATTGCTCTTCTCTATGCTCTTGGTTTGTTCAAAAGTCCTATAGCTCAAGGCTTACAATTTAAGTCAAGTGTTCAAGACTTCGTCATTTGTATAGAGATGTGCATTGCTTCTGTTGTTCATCTGTATGTTTTTCCTGCAAAGCCTTATGAACTGATGGGAGATCGCTTCCCCGGGAGTGTCTCAGTTCTTGGAGACTATGTATCTGCTGACTGTCCTTTGGATCCCGATGAAGTAAGGGACAGTGAACGTCCCACAAAGCTACGCCTTCCTACTCCTGACATTGATGTTAGGAGCGGAATGACCATAAGAGAAAGCGTTCGGGATGTTTTTATCGGTGGTGGGGGATATATTGTTAGCGATGTGAAGTTTACAGTAACACAGGCAGTTGAGCCTGTAGAGAAGGGGTTAACAAGGTTCAATGAGAAGCTACACAAGATATCTCAAAACATTAAGAGACATGACAAGGAGAAGAGGAAAACAAAGGATGACAGTTGTATTTCCACATCGTCACCTGTAGGGAAGGTGATTCGTGGGATAGATGACCCTCTCTTGAATGGAAGTATGAGCGATAGTGGTGTATCAAGGGCAAAGAAGCATCGCCGAAAATCAGGTTATACGAGTGCAGAAAGCGGTGGAGAGAGCAGCAGTGACCAAAGTTATGGTGGGTATCAGGTCAGAGGCCGCAGGTGGGTTACCAAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

487

Amino Acids

54.49

Weight (kDa)

8.52

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 23 - 302 1.6e-92 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AarI CACCTGC 1 cut(s) 1434
AasI GACNNNNNNGTC 1 cut(s) 721
Acc16I TGCGCA 1 cut(s) 250
Acc36I ACCTGC 1 cut(s) 1434
AccBSI CCGCTC 1 cut(s) 1053
AccI GTMKAC 2 cut(s) 390, 561
AccIII TCCGGA 1 cut(s) 398
AciI CCGC 4 cut(s) 135, 1053, 1389, 1441
AclWI GGATC 2 cut(s) 974, 987
AcsI RAATTY 3 cut(s) 485, 575, 619
AfaI GTAC 2 cut(s) 87, 644
AfiI CCNNNNNNNGG 1 cut(s) 1447
AgsI TTSAA 8 cut(s) 108, 445, 514, 548, 790, 833, 1177, 1312
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 6 cut(s) 50, 551, 686, 803, 1018, 1186
AluI AGCT 6 cut(s) 50, 551, 686, 803, 1018, 1186
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 4 cut(s) 522, 943, 945, 1207
AlwI GGATC 2 cut(s) 974, 987
Ama87I CYCGRG 1 cut(s) 929
Aor13HI TCCGGA 1 cut(s) 398
AoxI GGCC 2 cut(s) 39, 1438
ApeKI GCWGC 1 cut(s) 1401
ApoI RAATTY 3 cut(s) 485, 575, 619
Asp700I GAANNNNTTC 1 cut(s) 1074
AspLEI GCGC 1 cut(s) 251
AsuC2I CCSGG 2 cut(s) 930, 931
AsuHPI GGTGA 3 cut(s) 235, 1260, 1294
AvaI CYCGRG 1 cut(s) 929
AxyI CCTNAGG 1 cut(s) 150
BamHI GGATCC 1 cut(s) 979
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 1413
BccI CCATC 3 cut(s) 238, 563, 907
BceAI ACGGC 1 cut(s) 650
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 1 cut(s) 305
BciVI GTATCC 1 cut(s) 606
BcnI CCSGG 2 cut(s) 930, 931
BcoDI GTCTC 4 cut(s) 522, 943, 945, 1207
BfaI CTAG 3 cut(s) 533, 650, 683
BfmI CTRYAG 3 cut(s) 798, 1152, 1272
BfuAI ACCTGC 1 cut(s) 1434
BfuI GTATCC 1 cut(s) 606
BisI GCNGC 2 cut(s) 1402, 1441
BlsI GCNGC 2 cut(s) 1403, 1442
Bme1390I CCNGG 3 cut(s) 305, 930, 931
BmeT110I CYCGRG 1 cut(s) 929
BmiI GGNNCC 2 cut(s) 189, 981
BmrFI CCNGG 3 cut(s) 305, 930, 931
BmsI GCATC 3 cut(s) 87, 427, 1363
BpuEI CTTGAG 1 cut(s) 789
BpuMI CCSGG 2 cut(s) 930, 931
BsaJI CCNNGG 3 cut(s) 449, 928, 929
BsaWI WCCGGW 1 cut(s) 398
BsaXI ACNNNNNCTCC 2 cut(s) 942, 972
Bsc4I CCNNNNNNNGG 1 cut(s) 1447
Bse21I CCTNAGG 1 cut(s) 150
Bse3DI GCAATG 1 cut(s) 861
BseAI TCCGGA 1 cut(s) 398
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 449, 928, 929
BseGI GGATG 5 cut(s) 177, 418, 593, 1087, 1249
BseLI CCNNNNNNNGG 1 cut(s) 1447
BseMI GCAATG 1 cut(s) 861
BseMII CTCAG 3 cut(s) 141, 535, 954
BseXI GCAGC 1 cut(s) 1413
BsgI GTGCAG 1 cut(s) 1401
BshFI GGCC 2 cut(s) 41, 1440
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 929
BsiSI CCGG 2 cut(s) 399, 930
BslFI GGGAC 2 cut(s) 993, 1010
BslI CCNNNNNNNGG 1 cut(s) 1447
BsmAI GTCTC 4 cut(s) 522, 943, 945, 1207
BsmFI GGGAC 2 cut(s) 993, 1010
BsnI GGCC 2 cut(s) 41, 1440
BsoBI CYCGRG 1 cut(s) 929
Bsp1286I GDGCHC 1 cut(s) 129
Bsp13I TCCGGA 1 cut(s) 398
Bsp143I GATC 4 cut(s) 6, 22, 919, 979
Bsp19I CCATGG 1 cut(s) 449
BspACI CCGC 4 cut(s) 135, 1053, 1389, 1441
BspANI GGCC 2 cut(s) 41, 1440
BspCNI CTCAG 3 cut(s) 142, 534, 953
BspEI TCCGGA 1 cut(s) 398
BspLI GGNNCC 2 cut(s) 189, 981
BspMI ACCTGC 1 cut(s) 1434
BspPI GGATC 2 cut(s) 974, 987
BspQI GCTCTTC 1 cut(s) 771
BsrBI CCGCTC 1 cut(s) 1053
BsrDI GCAATG 1 cut(s) 861
BssECI CCNNGG 3 cut(s) 449, 928, 929
BssMI GATC 4 cut(s) 6, 22, 919, 979
BssNAI GTATAC 1 cut(s) 562
BssT1I CCWWGG 1 cut(s) 449
Bst1107I GTATAC 1 cut(s) 562
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 8 cut(s) 395, 412, 566, 647, 971, 1001, 1132, 1250
Bst6I CTCTTC 2 cut(s) 771, 1223
BstDEI CTNAG 4 cut(s) 68, 150, 521, 940
BstDSI CCRYGG 1 cut(s) 449
BstEII GGTNACC 1 cut(s) 1450
BstF5I GGATG 5 cut(s) 177, 418, 593, 1087, 1249
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 4 cut(s) 9, 25, 922, 982
BstMAI GTCTC 4 cut(s) 522, 943, 945, 1207
BstMBI GATC 4 cut(s) 6, 22, 919, 979
BstMWI GCNNNNNNNGC 2 cut(s) 47, 533
BstNI CCWGG 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 315
BstPI GGTNACC 1 cut(s) 1450
BstSCI CCNGG 3 cut(s) 303, 928, 929
BstSFI CTRYAG 3 cut(s) 798, 1152, 1272
BstV1I GCAGC 1 cut(s) 1413
BstX2I RGATCY 1 cut(s) 979
BstXI CCANNNNNNTGG 1 cut(s) 1418
BstYI RGATCY 1 cut(s) 979
BstZ17I GTATAC 1 cut(s) 562
Bsu36I CCTNAGG 1 cut(s) 150
BsuI GTATCC 1 cut(s) 606
BsuRI GGCC 2 cut(s) 41, 1440
BtgI CCRYGG 1 cut(s) 449
BtgZI GCGATG 2 cut(s) 1131, 1340
BtsCI GGATG 5 cut(s) 177, 418, 593, 1087, 1249
BtsI GCAGTG 2 cut(s) 615, 1411
BtsIMutI CAGTG 4 cut(s) 529, 615, 1006, 1411
BveI ACCTGC 1 cut(s) 1434
CfoI GCGC 1 cut(s) 251
Cfr9I CCCGGG 1 cut(s) 929
CsiI ACCWGGT 1 cut(s) 303
Csp6I GTAC 2 cut(s) 86, 643
CviAII CATG 9 cut(s) 57, 184, 192, 283, 312, 450, 633, 741, 1217
CviQI GTAC 2 cut(s) 86, 643
DdeI CTNAG 4 cut(s) 68, 150, 521, 940
DpnI GATC 4 cut(s) 8, 24, 921, 981
DpnII GATC 4 cut(s) 6, 22, 919, 979
DraI TTTAAA 1 cut(s) 580
DrdI GACNNNNNNGTC 1 cut(s) 721
DseDI GACNNNNNNGTC 1 cut(s) 721
Eam1104I CTCTTC 2 cut(s) 771, 1223
EarI CTCTTC 2 cut(s) 771, 1223
Eco130I CCWWGG 1 cut(s) 449
Eco32I GATATC 1 cut(s) 1197
Eco81I CCTNAGG 1 cut(s) 150
Eco88I CYCGRG 1 cut(s) 929
Eco91I GGTNACC 1 cut(s) 1450
EcoO65I GGTNACC 1 cut(s) 1450
EcoRII CCWGG 1 cut(s) 303
EcoRV GATATC 1 cut(s) 1197
EcoT14I CCWWGG 1 cut(s) 449
ErhI CCWWGG 1 cut(s) 449
FaeI CATG 9 cut(s) 60, 187, 195, 286, 315, 453, 636, 744, 1220
FaqI GGGAC 2 cut(s) 993, 1010
FatI CATG 9 cut(s) 56, 183, 191, 282, 311, 449, 632, 740, 1216
FblI GTMKAC 2 cut(s) 390, 561
Fnu4HI GCNGC 2 cut(s) 1402, 1441
FokI GGATG 5 cut(s) 164, 405, 600, 1094, 1256
Fsp4HI GCNGC 2 cut(s) 1402, 1441
FspBI CTAG 3 cut(s) 533, 650, 683
FspI TGCGCA 1 cut(s) 250
GlaI GCGC 1 cut(s) 250
GluI GCNGC 2 cut(s) 1402, 1441
HaeIII GGCC 2 cut(s) 41, 1440
HapII CCGG 2 cut(s) 399, 930
HhaI GCGC 1 cut(s) 251
Hin1II CATG 9 cut(s) 60, 187, 195, 286, 315, 453, 636, 744, 1220
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HincII GTYRAC 1 cut(s) 1167
HindII GTYRAC 1 cut(s) 1167
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 7 cut(s) 145, 206, 272, 376, 492, 613, 1285
HpaI GTTAAC 1 cut(s) 1167
HpaII CCGG 2 cut(s) 399, 930
HphI GGTGA 3 cut(s) 235, 1260, 1294
Hpy166II GTNNAC 6 cut(s) 226, 391, 562, 1004, 1128, 1167
Hpy188I TCNGA 3 cut(s) 491, 714, 1436
Hpy8I GTNNAC 6 cut(s) 226, 391, 562, 1004, 1128, 1167
HpyAV CCTTC 3 cut(s) 1034, 1153, 1273
HpyCH4III ACNGT 8 cut(s) 395, 412, 566, 647, 971, 1001, 1132, 1250
HpyCH4IV ACGT 1 cut(s) 1006
HpyCH4V TGCA 6 cut(s) 200, 311, 608, 861, 896, 1382
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 533
HpyF3I CTNAG 4 cut(s) 68, 150, 521, 940
HpySE526I ACGT 1 cut(s) 1006
Hsp92II CATG 9 cut(s) 60, 187, 195, 286, 315, 453, 636, 744, 1220
HspAI GCGC 1 cut(s) 249
Kpn2I TCCGGA 1 cut(s) 398
KspAI GTTAAC 1 cut(s) 1167
Kzo9I GATC 4 cut(s) 6, 22, 919, 979
LguI GCTCTTC 1 cut(s) 771
LmnI GCTCC 2 cut(s) 55, 1050
Lsp1109I GCAGC 1 cut(s) 1413
LweI GCATC 3 cut(s) 87, 427, 1363
MabI ACCWGGT 1 cut(s) 303
MaeI CTAG 3 cut(s) 533, 650, 683
MaeII ACGT 1 cut(s) 1006
MaeIII GTNAC 7 cut(s) 266, 412, 667, 1132, 1266, 1406, 1450
MalI GATC 4 cut(s) 8, 24, 921, 981
MbiI CCGCTC 1 cut(s) 1053
MboI GATC 4 cut(s) 6, 22, 919, 979
MboII GAAGA 6 cut(s) 16, 107, 309, 338, 758, 1240
MfeI CAATTG 1 cut(s) 759
MflI RGATCY 1 cut(s) 979
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 7 cut(s) 168, 252, 485, 575, 619, 759, 815
MlyI GAGTC 2 cut(s) 215, 370
MnlI CCTC 5 cut(s) 145, 191, 1224, 1314, 1430
MroI TCCGGA 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 1074
MseI TTAA 8 cut(s) 167, 437, 579, 720, 819, 1166, 1209, 1462
MslI CAYNNNNRTG 2 cut(s) 182, 882
MspI CCGG 2 cut(s) 399, 930
MspR9I CCNGG 3 cut(s) 305, 930, 931
MunI CAATTG 1 cut(s) 759
MvaI CCWGG 1 cut(s) 305
MwoI GCNNNNNNNGC 2 cut(s) 47, 533
NciI CCSGG 2 cut(s) 930, 931
NcoI CCATGG 1 cut(s) 449
NdeII GATC 4 cut(s) 6, 22, 919, 979
NlaIII CATG 9 cut(s) 60, 187, 195, 286, 315, 453, 636, 744, 1220
NlaIV GGNNCC 2 cut(s) 189, 981
NmuCI GTSAC 3 cut(s) 412, 1266, 1406
NsbI TGCGCA 1 cut(s) 250
NspI RCATGY 1 cut(s) 315
PaqCI CACCTGC 1 cut(s) 1434
PciSI GCTCTTC 1 cut(s) 771
PdmI GAANNNNTTC 1 cut(s) 1074
PfeI GAWTC 5 cut(s) 145, 272, 492, 613, 1285
PflFI GACNNNGTC 1 cut(s) 839
PkrI GCNGC 2 cut(s) 1403, 1442
PleI GAGTC 2 cut(s) 214, 370
PpsI GAGTC 2 cut(s) 214, 370
PsiI TTATAA 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 303
PspEI GGTNACC 1 cut(s) 1450
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 2 cut(s) 189, 981
PsuI RGATCY 1 cut(s) 979
PsyI GACNNNGTC 1 cut(s) 839
RsaI GTAC 2 cut(s) 87, 644
RsaNI GTAC 2 cut(s) 86, 643
RseI CAYNNNNRTG 2 cut(s) 182, 882
SapI GCTCTTC 1 cut(s) 771
SaqAI TTAA 8 cut(s) 167, 437, 579, 720, 819, 1166, 1209, 1462
SatI GCNGC 2 cut(s) 1402, 1441
Sau3AI GATC 4 cut(s) 6, 22, 919, 979
SchI GAGTC 2 cut(s) 215, 370
ScrFI CCNGG 3 cut(s) 305, 930, 931
SduI GDGCHC 1 cut(s) 129
SexAI ACCWGGT 1 cut(s) 303
SfaNI GCATC 3 cut(s) 87, 427, 1363
SfcI CTRYAG 3 cut(s) 798, 1152, 1272
SmaI CCCGGG 1 cut(s) 931
SmiI ATTTAAAT 1 cut(s) 580
SmiMI CAYNNNNRTG 2 cut(s) 182, 882
SmlI CTYRAG 1 cut(s) 804
SmoI CTYRAG 1 cut(s) 804
Sse9I AATT 7 cut(s) 168, 252, 485, 575, 619, 759, 815
SsiI CCGC 4 cut(s) 135, 1053, 1389, 1441
SspMI CTAG 3 cut(s) 533, 650, 683
StyD4I CCNGG 3 cut(s) 303, 928, 929
StyI CCWWGG 1 cut(s) 449
SwaI ATTTAAAT 1 cut(s) 580
TaaI ACNGT 8 cut(s) 395, 412, 566, 647, 971, 1001, 1132, 1250
TaiI ACGT 1 cut(s) 1009
TaqI TCGA 3 cut(s) 21, 339, 725
TasI AATT 7 cut(s) 168, 252, 485, 575, 619, 759, 815
TatI WGTACW 2 cut(s) 85, 642
TauI GCSGC 1 cut(s) 1443
TfiI GAWTC 5 cut(s) 145, 272, 492, 613, 1285
Tru1I TTAA 8 cut(s) 167, 437, 579, 720, 819, 1166, 1209, 1462
Tru9I TTAA 8 cut(s) 167, 437, 579, 720, 819, 1166, 1209, 1462
TscAI CASTG 4 cut(s) 529, 615, 1006, 1411
TseFI GTSAC 3 cut(s) 412, 1266, 1406
TseI GCWGC 1 cut(s) 1401
Tsp45I GTSAC 3 cut(s) 412, 1266, 1406
TspDTI ATGAA 6 cut(s) 17, 446, 693, 866, 921, 1002
TspMI CCCGGG 1 cut(s) 929
TspRI CASTG 4 cut(s) 529, 615, 1006, 1411
Tth111I GACNNNGTC 1 cut(s) 839
XapI RAATTY 3 cut(s) 485, 575, 619
XceI RCATGY 1 cut(s) 315
XcmI CCANNNNNNNNNTGG 1 cut(s) 1418
XmaI CCCGGG 1 cut(s) 929
XmiI GTMKAC 2 cut(s) 390, 561
XmnI GAANNNNTTC 1 cut(s) 1074
XspI CTAG 3 cut(s) 533, 650, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.