Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0003338

Physical Location & Seq

Genomic Coordinates Forward (+)

13395134 .. 13399110

Gene Structure 3977 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000011863

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGGCGATGTACGTCATTTCACGGCGAGTAACCGCCGGATCGACGCAGTTAACGGCGTTCCGTTACGCCACTACTTGCTGGAGGAACTATTCGACGTCGTTTCGCGAGGAAAGGGACACCTTTGGACCCATTCTCGTCCCCTCAGACAAACTATGGGGAGCTCAAACTCAGAGATCGTTGCAGAACTTCGATATCGGCGGCGACCGTGAACGAATGCCGGAGCCTATTATTCGCGCCTTCGGTGTCCTCAAGAAATGTGCTGCCAAGGTGAACATGGAGTATGGTCTTGACCAATCTTTAGGGAAAGCTATAATGCAGGCTGCACAAGAAGTGGCCGAGGGGAAGCTAAATCAGCACTTTCCACTTGTTGTGTGGCAAACTGGTAGTGGAACACAAAGCAACATGAATGCCAATGAGGTCATTGCAAACAGAGCAGCTGAGATACTTGGACATAAGCGCGGCGAGAAGTTTGTGCATCCGAATGACCATGTTAATAGATCACAGTCTTCTAATGACACTTTCCCAACTGTCATGCACATTGCAACTGCGATGGAAGTAAATTCAAGACTGGTACCAAATTTGAAAACATTGCATTCTTCTCTACAACAAAAGTCGGATGAGTTCAAAGATATTGTTAAAATTGGACGCACTCACACACAAGATGCAACACCTTTGACTCTTGGGCAAGAGTTTAGCGGCTATTCTACACAAGTGAAGTATGGAATCGAGCGAGTCTTGTGCACTCTACCCCACATGTATCAGCTGGCACAGGGTGGTACTGCTGTTGGGACTGGATTGAATACAAAGAAAGGATTTGATGTAAAGATAGCTGCAGCAGTAGCTGATGAAACAAACTTACCCTTTGTCACTGCAGAAAACAAGTTTGAAGCTTTGGCTGCACATGATGCTTTTGTGGAAACTAGTGGAGCCCTGAATACAGTTGCTACTTCTCTGATGAAAATAGCTAATGACATACGTTTATTAGGAAGTGGTCCACGATGTGGTCTTGGTGAACTCATTCTTCCTGAAAATGAGCCTGGAAGCAGCATTATGCCGGGGAAGGTTAACCCAACCCAGTGTGAGGCTATCACAATGGTTTGTGCTCAGGTTATGGGTAACAATGTGGCCATTACAGTAGGTGGATCTAATGGTCATTTTGAACTAAATGTATTCAAACCAATGATTGCTAGTGCTCTCCTACACTCTGTTAGATTACTTGGGGATGCATCTGCTTCCTTTGAAAAGAACTGTGTTAGAGGAATTGAAGCCAACAGGGAAAGAATCTCAAAGTTACTTCATGAGTCACTGATGCTGGTCACGTCCTTGAATCCTGACGCTGCATTAAAACTTGGGGTGCTAACGAGTGAAGAGTTTGATAATCTTGTGGTGCCCGAGAAGATGATTGGCCCGTCTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004333 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006106 GO:0006108 GO:0006807 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0008152 GO:0009060 GO:0009266 GO:0009409 GO:0009628 GO:0009631 GO:0009651 GO:0009856 GO:0009987 GO:0010109 GO:0015980 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017144 GO:0019222 GO:0019752 GO:0022414 GO:0031323 GO:0032501 GO:0032502 GO:0042126 GO:0042128 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0045333 GO:0048856 GO:0048868 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0055114 GO:0065007 GO:0071704 GO:0071941 GO:0072350 GO:2001057

KEGG Pathways

Metabolic & Signaling

ko00020 ko00620 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko04934 ko05200 ko05211 map00020 map00620 map00720 map01100 map01110 map01120 map01130 map01200 map04934 map05200 map05211

Pfam Domains

Protein Families

FumaraseC_C Lyase_1

Protein Analysis

473

Amino Acids

51.31

Weight (kDa)

6.65

Isoelectric Point (pI)

31.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lyase_1	PF00206	44 - 372	1.4e-116	Lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003338)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47510 AT2G47510 AT2G47510 AT5G50950 AT5G50950 AT5G50950 AT5G50950
fragaria_vesca	FvH4_2g26121 FvH4_2g26121 FvH4_2g26140 FvH4_2g26140
malus_domestica	MD00G1140200.v1.1 MD03G1292300.v1.1
prunus_persica	Prupe.8G265300_v2.0.a1 Prupe.8G265300_v2.0.a1 Prupe.8G265300_v2.0.a1
pyrus_communis	pycom03g23150 pycom11g27280
rosa_chinensis	RchiOBHm_Chr6g0294511 RchiOBHm_Chr6g0294521
rosa_laevigata	RLG00000011863
rosa_multiflora	Rmu_ssc0000248.1_g000007
rosa_roxburghii	Rroxscaffold_7G00173260 Rroxscaffold_7G00173270
rosa_rugosa	Rorug06G0245500 Rorug06G0245500
rosa_samantha	Rh6AG357800 Rh6AG357900 Rh6BG364700 Rh6BG364800 Rh6CG371800 Rh6CG371900 Rh6DG358600 Rh6DG358700
rosa_wichuraiana	Rw6G031210 Rw6G031220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	98
Acc65I	GGTACC	1 cut(s)	571
AccB1I	GGYRCC	2 cut(s)	571, 1387
AccB7I	CCANNNNNTGG	1 cut(s)	1001
AccII	CGCG	3 cut(s)	105, 234, 459
AciI	CCGC	4 cut(s)	33, 198, 459, 696
AclWI	GGATC	2 cut(s)	46, 1150
AcoI	YGGCCR	2 cut(s)	333, 1125
AcsI	RAATTY	2 cut(s)	559, 577
AcyI	GRCGYC	1 cut(s)	95
AdeI	CACNNNGTG	2 cut(s)	773, 1001
AfaI	GTAC	3 cut(s)	11, 573, 778
AfiI	CCNNNNNNNGG	2 cut(s)	1001, 1081
AflIII	ACRYGT	1 cut(s)	753
AhlI	ACTAGT	1 cut(s)	920
AjiI	CACGTC	1 cut(s)	1320
AjnI	CCWGG	1 cut(s)	1036
AluBI	AGCT	9 cut(s)	161, 308, 346, 437, 763, 830, 842, 890, 965
AluI	AGCT	9 cut(s)	161, 308, 346, 437, 763, 830, 842, 890, 965
Alw21I	GWGCWC	4 cut(s)	163, 743, 1105, 1195
Alw44I	GTGCAC	1 cut(s)	739
AlwI	GGATC	2 cut(s)	46, 1150
AlwNI	CAGNNNCTG	1 cut(s)	842
Ama87I	CYCGRG	1 cut(s)	1391
AoxI	GGCC	3 cut(s)	333, 1125, 1405
ApaLI	GTGCAC	1 cut(s)	739
ApeKI	GCWGC	8 cut(s)	260, 320, 434, 830, 833, 896, 1044, 1337
ApoI	RAATTY	2 cut(s)	559, 577
Asp700I	GAANNNNTTC	1 cut(s)	1017
Asp718I	GGTACC	1 cut(s)	571
AspLEI	GCGC	2 cut(s)	236, 459
AspS9I	GGNCC	3 cut(s)	125, 992, 1406
AsuC2I	CCSGG	1 cut(s)	1056
AsuHPI	GGTGA	2 cut(s)	280, 1022
AvaI	CYCGRG	1 cut(s)	1391
AvaII	GGWCC	2 cut(s)	125, 992
BaeGI	GKGCMC	2 cut(s)	743, 1392
BaeI	ACNNNNGTAYC	2 cut(s)	768, 801
BalI	TGGCCA	1 cut(s)	1127
BanI	GGYRCC	2 cut(s)	571, 1387
BanII	GRGCYC	2 cut(s)	163, 931
BbsI	GAAGAC	1 cut(s)	498
Bbv12I	GWGCWC	4 cut(s)	163, 743, 1105, 1195
BbvI	GCAGC	8 cut(s)	247, 307, 446, 817, 845, 883, 1056, 1324
BccI	CCATC	1 cut(s)	544
BceAI	ACGGC	2 cut(s)	38, 69
BcgI	CGANNNNNNTGC	2 cut(s)	720, 754
BciT130I	CCWGG	1 cut(s)	1038
BcnI	CCSGG	1 cut(s)	1056
BcuI	ACTAGT	1 cut(s)	920
BfaI	CTAG	2 cut(s)	921, 1188
BfmI	CTRYAG	2 cut(s)	831, 870
Bme1390I	CCNGG	2 cut(s)	1038, 1056
Bme18I	GGWCC	2 cut(s)	125, 992
BmeT110I	CYCGRG	1 cut(s)	1391
BmgBI	CACGTC	1 cut(s)	1320
BmgT120I	GGNCC	3 cut(s)	125, 992, 1406
BmiI	GGNNCC	5 cut(s)	127, 222, 573, 928, 1389
BmrFI	CCNGG	2 cut(s)	1038, 1056
BmrI	ACTGGG	1 cut(s)	1069
BmsI	GCATC	6 cut(s)	484, 652, 895, 1213, 1235, 1299
BmuI	ACTGGG	1 cut(s)	1069
BpiI	GAAGAC	1 cut(s)	498
BpmI	CTGGAG	1 cut(s)	100
Bpu10I	CCTNAGC	1 cut(s)	1104
BpuEI	CTTGAG	1 cut(s)	233
BpuMI	CCSGG	1 cut(s)	1056
BsaHI	GRCGYC	1 cut(s)	95
BsaJI	CCNNGG	3 cut(s)	264, 336, 1055
Bsc4I	CCNNNNNNNGG	2 cut(s)	1001, 1081
Bse1I	ACTGG	4 cut(s)	385, 573, 796, 1075
Bse3DI	GCAATG	3 cut(s)	420, 537, 587
BseBI	CCWGG	1 cut(s)	1038
BseDI	CCNNGG	3 cut(s)	264, 336, 1055
BseGI	GGATG	3 cut(s)	475, 622, 1228
BseLI	CCNNNNNNNGG	2 cut(s)	1001, 1081
BseMI	GCAATG	3 cut(s)	420, 537, 587
BseMII	CTCAG	4 cut(s)	156, 182, 429, 1118
BseNI	ACTGG	4 cut(s)	385, 573, 796, 1075
BseSI	GKGCMC	2 cut(s)	743, 1392
BseXI	GCAGC	8 cut(s)	247, 307, 446, 817, 845, 883, 1056, 1324
BsgI	GTGCAG	2 cut(s)	306, 882
Bsh1236I	CGCG	3 cut(s)	105, 234, 459
Bsh1285I	CGRYCG	1 cut(s)	205
BshFI	GGCC	3 cut(s)	335, 1127, 1407
BshNI	GGYRCC	2 cut(s)	571, 1387
BsiEI	CGRYCG	1 cut(s)	205
BsiHKAI	GWGCWC	4 cut(s)	163, 743, 1105, 1195
BsiHKCI	CYCGRG	1 cut(s)	1391
BsiSI	CCGG	3 cut(s)	36, 218, 1055
BslFI	GGGAC	3 cut(s)	122, 128, 802
BslI	CCNNNNNNNGG	2 cut(s)	1001, 1081
BsmFI	GGGAC	3 cut(s)	122, 128, 802
BsmI	GAATGC	3 cut(s)	219, 412, 592
BsnI	GGCC	3 cut(s)	335, 1127, 1407
BsoBI	CYCGRG	1 cut(s)	1391
Bsp1286I	GDGCHC	6 cut(s)	163, 743, 931, 1105, 1195, 1392
Bsp143I	GATC	4 cut(s)	38, 173, 497, 1142
Bsp68I	TCGCGA	1 cut(s)	105
BspACI	CCGC	4 cut(s)	33, 198, 459, 696
BspANI	GGCC	3 cut(s)	335, 1127, 1407
BspCNI	CTCAG	4 cut(s)	155, 181, 430, 1117
BspFNI	CGCG	3 cut(s)	105, 234, 459
BspHI	TCATGA	1 cut(s)	1297
BspLI	GGNNCC	5 cut(s)	127, 222, 573, 928, 1389
BspMAI	CTGCAG	2 cut(s)	835, 874
BspPI	GGATC	2 cut(s)	46, 1150
BspT107I	GGYRCC	2 cut(s)	571, 1387
BsrDI	GCAATG	3 cut(s)	420, 537, 587
BsrI	ACTGG	4 cut(s)	385, 573, 796, 1075
BssECI	CCNNGG	3 cut(s)	264, 336, 1055
BssMI	GATC	4 cut(s)	38, 173, 497, 1142
BssNI	GRCGYC	1 cut(s)	95
BssT1I	CCWWGG	1 cut(s)	264
Bst2UI	CCWGG	1 cut(s)	1038
Bst4CI	ACNGT	6 cut(s)	206, 504, 529, 940, 1135, 1250
Bst6I	CTCTTC	1 cut(s)	1362
BstACI	GRCGYC	1 cut(s)	95
BstAPI	GCANNNNNTGC	1 cut(s)	905
BstC8I	GCNNGC	2 cut(s)	318, 765
BstDEI	CTNAG	4 cut(s)	142, 168, 438, 1104
BstF5I	GGATG	3 cut(s)	475, 622, 1228
BstFNI	CGCG	3 cut(s)	105, 234, 459
BstHHI	GCGC	2 cut(s)	236, 459
BstKTI	GATC	4 cut(s)	41, 176, 500, 1145
BstMBI	GATC	4 cut(s)	38, 173, 497, 1142
BstMCI	CGRYCG	1 cut(s)	205
BstMWI	GCNNNNNNNGC	5 cut(s)	352, 431, 839, 896, 905
BstNI	CCWGG	1 cut(s)	1038
BstNSI	RCATGY	1 cut(s)	757
BstSCI	CCNGG	2 cut(s)	1036, 1054
BstSFI	CTRYAG	2 cut(s)	831, 870
BstSLI	GKGCMC	2 cut(s)	743, 1392
BstUI	CGCG	3 cut(s)	105, 234, 459
BstV1I	GCAGC	8 cut(s)	247, 307, 446, 817, 845, 883, 1056, 1324
BstV2I	GAAGAC	1 cut(s)	498
BstX2I	RGATCY	1 cut(s)	1142
BstYI	RGATCY	1 cut(s)	1142
BsuRI	GGCC	3 cut(s)	335, 1127, 1407
BtgZI	GCGATG	2 cut(s)	20, 563
BtrI	CACGTC	1 cut(s)	1320
BtsCI	GGATG	3 cut(s)	475, 622, 1228
BtsI	GCAGTG	1 cut(s)	867
BtsIMutI	CAGTG	3 cut(s)	867, 1082, 1304
BtuMI	TCGCGA	1 cut(s)	105
Cac8I	GCNNGC	2 cut(s)	318, 765
CaiI	CAGNNNCTG	1 cut(s)	842
CciI	TCATGA	1 cut(s)	1297
CfoI	GCGC	2 cut(s)	236, 459
Cfr13I	GGNCC	3 cut(s)	125, 992, 1406
CseI	GACGC	3 cut(s)	52, 654, 1343
Csp6I	GTAC	3 cut(s)	10, 572, 777
CviAII	CATG	7 cut(s)	274, 403, 488, 532, 754, 902, 1298
CviQI	GTAC	3 cut(s)	10, 572, 777
DdeI	CTNAG	4 cut(s)	142, 168, 438, 1104
DpnI	GATC	4 cut(s)	40, 175, 499, 1144
DpnII	GATC	4 cut(s)	38, 173, 497, 1142
DraIII	CACNNNGTG	2 cut(s)	773, 1001
EaeI	YGGCCR	2 cut(s)	333, 1125
Eam1104I	CTCTTC	1 cut(s)	1362
EarI	CTCTTC	1 cut(s)	1362
Ecl136II	GAGCTC	1 cut(s)	161
Eco130I	CCWWGG	1 cut(s)	264
Eco24I	GRGCYC	2 cut(s)	163, 931
Eco32I	GATATC	1 cut(s)	193
Eco47I	GGWCC	2 cut(s)	125, 992
Eco53kI	GAGCTC	1 cut(s)	161
Eco88I	CYCGRG	1 cut(s)	1391
EcoICRI	GAGCTC	1 cut(s)	161
EcoRII	CCWGG	1 cut(s)	1036
EcoRV	GATATC	1 cut(s)	193
EcoT14I	CCWWGG	1 cut(s)	264
EcoT22I	ATGCAT	1 cut(s)	1228
EcoT38I	GRGCYC	2 cut(s)	163, 931
ErhI	CCWWGG	1 cut(s)	264
FaeI	CATG	7 cut(s)	277, 406, 491, 535, 757, 905, 1301
FaqI	GGGAC	3 cut(s)	122, 128, 802
FatI	CATG	7 cut(s)	273, 402, 487, 531, 753, 901, 1297
FokI	GGATG	3 cut(s)	462, 629, 1235
FriOI	GRGCYC	2 cut(s)	163, 931
FspBI	CTAG	2 cut(s)	921, 1188
GlaI	GCGC	2 cut(s)	235, 458
GsuI	CTGGAG	1 cut(s)	100
HaeIII	GGCC	3 cut(s)	335, 1127, 1407
HapII	CCGG	3 cut(s)	36, 218, 1055
HgaI	GACGC	3 cut(s)	52, 654, 1343
HhaI	GCGC	2 cut(s)	236, 459
Hin1I	GRCGYC	1 cut(s)	95
Hin1II	CATG	7 cut(s)	277, 406, 491, 535, 757, 905, 1301
Hin6I	GCGC	2 cut(s)	234, 457
HinP1I	GCGC	2 cut(s)	234, 457
HincII	GTYRAC	2 cut(s)	51, 1066
HindII	GTYRAC	2 cut(s)	51, 1066
HindIII	AAGCTT	1 cut(s)	888
HinfI	GANTC	6 cut(s)	676, 723, 732, 1281, 1301, 1327
HpaI	GTTAAC	2 cut(s)	51, 1066
HpaII	CCGG	3 cut(s)	36, 218, 1055
HphI	GGTGA	2 cut(s)	280, 1022
Hpy166II	GTNNAC	7 cut(s)	51, 209, 271, 741, 995, 1013, 1066
Hpy188I	TCNGA	6 cut(s)	145, 171, 480, 616, 954, 1414
Hpy188III	TCNNGA	7 cut(s)	104, 250, 287, 564, 1025, 1298, 1331
Hpy8I	GTNNAC	7 cut(s)	51, 209, 271, 741, 995, 1013, 1066
Hpy99I	CGWCG	3 cut(s)	46, 97, 100
HpyAV	CCTTC	2 cut(s)	247, 1054
HpyCH4III	ACNGT	6 cut(s)	206, 504, 529, 940, 1135, 1250
HpyCH4IV	ACGT	4 cut(s)	12, 95, 976, 1319
HpyF10VI	GCNNNNNNNGC	5 cut(s)	352, 431, 839, 896, 905
HpyF3I	CTNAG	4 cut(s)	142, 168, 438, 1104
HpySE526I	ACGT	4 cut(s)	12, 95, 976, 1319
Hsp92I	GRCGYC	1 cut(s)	95
Hsp92II	CATG	7 cut(s)	277, 406, 491, 535, 757, 905, 1301
HspAI	GCGC	2 cut(s)	234, 457
KpnI	GGTACC	1 cut(s)	575
KspAI	GTTAAC	2 cut(s)	51, 1066
Kzo9I	GATC	4 cut(s)	38, 173, 497, 1142
LmnI	GCTCC	3 cut(s)	158, 220, 926
Lsp1109I	GCAGC	8 cut(s)	247, 307, 446, 817, 845, 883, 1056, 1324
LweI	GCATC	6 cut(s)	484, 652, 895, 1213, 1235, 1299
MaeI	CTAG	2 cut(s)	921, 1188
MaeII	ACGT	4 cut(s)	12, 95, 976, 1319
MaeIII	GTNAC	7 cut(s)	28, 62, 865, 1115, 1290, 1302, 1315
MalI	GATC	4 cut(s)	40, 175, 499, 1144
MboI	GATC	4 cut(s)	38, 173, 497, 1142
MboII	GAAGA	5 cut(s)	498, 588, 1013, 1379, 1408
MflI	RGATCY	1 cut(s)	1142
MhlI	GDGCHC	6 cut(s)	163, 743, 931, 1105, 1195, 1392
MlsI	TGGCCA	1 cut(s)	1127
MluCI	AATT	4 cut(s)	559, 577, 639, 1260
MluNI	TGGCCA	1 cut(s)	1127
MlyI	GAGTC	3 cut(s)	670, 741, 1310
MmeI	TCCRAC	1 cut(s)	594
MnlI	CCTC	8 cut(s)	75, 100, 151, 257, 331, 409, 1075, 1250
Mox20I	TGGCCA	1 cut(s)	1127
Mph1103I	ATGCAT	1 cut(s)	1228
MroXI	GAANNNNTTC	1 cut(s)	1017
MscI	TGGCCA	1 cut(s)	1127
MseI	TTAA	5 cut(s)	50, 492, 636, 1065, 1343
MslI	CAYNNNNRTG	1 cut(s)	480
Msp20I	TGGCCA	1 cut(s)	1127
MspA1I	CMGCKG	2 cut(s)	437, 763
MspI	CCGG	3 cut(s)	36, 218, 1055
MspR9I	CCNGG	2 cut(s)	1038, 1056
Mva1269I	GAATGC	3 cut(s)	219, 412, 592
MvaI	CCWGG	1 cut(s)	1038
MvnI	CGCG	3 cut(s)	105, 234, 459
MwoI	GCNNNNNNNGC	5 cut(s)	352, 431, 839, 896, 905
NciI	CCSGG	1 cut(s)	1056
NdeII	GATC	4 cut(s)	38, 173, 497, 1142
NlaIII	CATG	7 cut(s)	277, 406, 491, 535, 757, 905, 1301
NlaIV	GGNNCC	5 cut(s)	127, 222, 573, 928, 1389
NmeAIII	GCCGAG	1 cut(s)	361
NmuCI	GTSAC	3 cut(s)	865, 1302, 1315
NruI	TCGCGA	1 cut(s)	105
NsiI	ATGCAT	1 cut(s)	1228
NspI	RCATGY	1 cut(s)	757
PagI	TCATGA	1 cut(s)	1297
PciI	ACATGT	1 cut(s)	753
PctI	GAATGC	3 cut(s)	219, 412, 592
PdmI	GAANNNNTTC	1 cut(s)	1017
PfeI	GAWTC	3 cut(s)	723, 1281, 1327
PflMI	CCANNNNNTGG	1 cut(s)	1001
PleI	GAGTC	3 cut(s)	670, 740, 1309
PpsI	GAGTC	3 cut(s)	670, 740, 1309
PscI	ACATGT	1 cut(s)	753
Psp124BI	GAGCTC	1 cut(s)	163
Psp6I	CCWGG	1 cut(s)	1036
PspGI	CCWGG	1 cut(s)	1036
PspN4I	GGNNCC	5 cut(s)	127, 222, 573, 928, 1389
PspPI	GGNCC	3 cut(s)	125, 992, 1406
PstI	CTGCAG	2 cut(s)	835, 874
PstNI	CAGNNNCTG	1 cut(s)	842
PsuI	RGATCY	1 cut(s)	1142
PvuII	CAGCTG	2 cut(s)	437, 763
RruI	TCGCGA	1 cut(s)	105
RsaI	GTAC	3 cut(s)	11, 573, 778
RsaNI	GTAC	3 cut(s)	10, 572, 777
RseI	CAYNNNNRTG	1 cut(s)	480
SacI	GAGCTC	1 cut(s)	163
SaqAI	TTAA	5 cut(s)	50, 492, 636, 1065, 1343
Sau3AI	GATC	4 cut(s)	38, 173, 497, 1142
Sau96I	GGNCC	3 cut(s)	125, 992, 1406
SchI	GAGTC	3 cut(s)	670, 741, 1310
ScrFI	CCNGG	2 cut(s)	1038, 1056
SduI	GDGCHC	6 cut(s)	163, 743, 931, 1105, 1195, 1392
SfaNI	GCATC	6 cut(s)	484, 652, 895, 1213, 1235, 1299
SfcI	CTRYAG	2 cut(s)	831, 870
SinI	GGWCC	2 cut(s)	125, 992
SmiMI	CAYNNNNRTG	1 cut(s)	480
SmlI	CTYRAG	1 cut(s)	248
SmoI	CTYRAG	1 cut(s)	248
SpeI	ACTAGT	1 cut(s)	920
Sse9I	AATT	4 cut(s)	559, 577, 639, 1260
SsiI	CCGC	4 cut(s)	33, 198, 459, 696
SspMI	CTAG	2 cut(s)	921, 1188
SstI	GAGCTC	1 cut(s)	163
StyD4I	CCNGG	2 cut(s)	1036, 1054
StyI	CCWWGG	1 cut(s)	264
TaaI	ACNGT	6 cut(s)	206, 504, 529, 940, 1135, 1250
TaiI	ACGT	4 cut(s)	15, 98, 979, 1322
TaqI	TCGA	4 cut(s)	41, 92, 189, 726
TasI	AATT	4 cut(s)	559, 577, 639, 1260
TauI	GCSGC	3 cut(s)	201, 462, 699
TfiI	GAWTC	3 cut(s)	723, 1281, 1327
Tru1I	TTAA	5 cut(s)	50, 492, 636, 1065, 1343
Tru9I	TTAA	5 cut(s)	50, 492, 636, 1065, 1343
TscAI	CASTG	3 cut(s)	874, 1082, 1311
TseFI	GTSAC	3 cut(s)	865, 1302, 1315
TseI	GCWGC	8 cut(s)	260, 320, 434, 830, 833, 896, 1044, 1337
Tsp45I	GTSAC	3 cut(s)	865, 1302, 1315
TspDTI	ATGAA	4 cut(s)	419, 861, 971, 1286
TspGWI	ACGGA	1 cut(s)	50
TspRI	CASTG	3 cut(s)	874, 1082, 1311
Van91I	CCANNNNNTGG	1 cut(s)	1001
VneI	GTGCAC	1 cut(s)	739
VpaK11BI	GGWCC	2 cut(s)	125, 992
XapI	RAATTY	2 cut(s)	559, 577
XceI	RCATGY	1 cut(s)	757
XcmI	CCANNNNNNNNNTGG	2 cut(s)	271, 369
XmnI	GAANNNNTTC	1 cut(s)	1017
XspI	CTAG	2 cut(s)	921, 1188
ZraI	GACGTC	1 cut(s)	96
Zsp2I	ATGCAT	1 cut(s)	1228

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.