Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005152

Physical Location & Seq

Genomic Coordinates Forward (+)

37125531 .. 37131852

Gene Structure 6322 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1295400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGAAGGACATCGACTTCAGCGCTGAAAAAGAGCTCGCCAAGGATTTTCTTTCCAACTTTCCCGATTCGAATGGCGAACCCAAATACATGCGCATCCTTCAACAAGTTGCAAACCGCAAATTTCGAGCTATCGAGATTGATATTGAGGACTTGTTTAGTTACGCAGAATTGGATGAAGAATTCCGCAATCGAGTTCAAGAAAATACTCGGCGTTATATTGGTATTTTTGCTGATGCTATTGATGAGCTTATGCCGGAGCCCACAGAGGCATTTACAGATGATGATCATGACATACTCATGACTCAAAGGTCTGATGATGGACCAGAGAATATGGATGGTCCGGATCCACACCAGAAGATGCCTCCTGAAATCAAGCGCTACTTTGAAGTTTACATAAGAGCATCTTCAAGTGGAAAGGTGAAGCCATATGCAATTAGGGAGGTCAAGGCTTCACATATTGGTCAGCTTGTAAGGATATCAGGTATTGTGACGCGGTGTTCAGATGTTAAGCCATTGATGCAGGTAGCTGTCTATACATGTGAAGAATGTGGTTTTGAGATTTACCAGGAAGTAACTGCTCGAGTCTTTATGCCACTTTTTGAATGCCCATCCAGGCGTTGTATAACAAATAGAACAAAGGGGAACCTTATTCTTCAACTCAGGGCATCAAAGTTCTTGAAATTTCAGGAGGCAAAGATACAAGAGTTGTCTGAAGATGTTCCAAAAGGCCATATTCCACGGACAATGACCGTTCACTTCCGGGGTGAACTCACAAGAAAGGTAGCTCCGGGTGATGTTGTGAAACTATCTGGGATTTTTCTTCCTATTCCTTATACTGGTTTCAGAGCAATGCGGGCTGGCTTAGTTGCTGATACTTACTTAGAAGCCATGTCCATCACACATACTAAGAAAAAATATGAAGATTATGAACTTATAGGAGATGAGGAAGAACAAGTTGCACGTTTGGCTGAGGATGGTAACATATATGAAAAGCTGGCTCGATCTTTGGCACCTGAAATATACGGGCATGAAGATATCAAAAAGGCTCTGCTTCTGCTCCTAGTGGGCGCTCCTCACAGGAAACTAAAAGATGGAATGAAGATTAGAGGAGATTTACATATTTGTCTGATGGGTGATCCTGGAGTTGCAAAGAGCCAGCTTCTCAAACACATAATTAATGTAGCACCCAGGGGAGTATATACAACTGGCAAAGGAAGCAGTGGAGTTGGTCTAACTGCTGCTGTTCAGAAAGATCCCGTCACAAATGAAATGGTCCTGGAAGGAGGAGCATTGGTGCTAGCGGATATGGGAATATGTGCTATTGATGAGTTTGATAAGATGGATGAATTAGATCGTACAGCCATACATGAAGTTATGGAGCAGCAGACTGTCAGCATTGCCAAGGCTGGGATCACTACGTCCTTGAATGCAAGAACTGCTGTTCTTGCTGCAGCTAATCCAGCATGGGGGAGATATGACCTACGTAGAACTCCTGCTGAAAACATTAATCTTCCACCGGCCCTTTTGTCAAGATTTGATCTTTTATGGTTAATCCTGGATCGAGCAGATATGGATAGTGATCTTGAGATGGCTAGGCATGTTGTCTATGTTCATCAGAATAAAGAATCTCCGGCTCTTGGTTTCACTCCACTTGAGCCATCTGTTATTCGGGCATATATTTCTGCAGTGAGAAGATTGTCTCCTTCAGTCCCACAGGAACTTGAGGAGTATATTGCTAGTGCCTACTCTAGCATTCGGCAAGAAGAAGCTAAATCGAATGCACCCCATTCCTATACAACTGTGAGAACTCTGCTCAGCATTCTTCGAATATCAGCTGCAATAGCAAGACTCCGACATTCTGAAACTGTGGCTCAATCTGATGTGGATGAGGCATTAAGGCTGATGCATATGTCGAAGTTCTCTTTGTACTCAGATGATCGCCAGAAATCTGGTCTGGATCCTATTGGAGATATCTATTCAATTTTGCGAGATGAGTCTGAGACGACGGGCAAGCAGGATGTGAGCTATGCACATGCACTAAATCTGATTTCTAGGAAGGGATACAGTGAAGCCCAACTGAAACAATGTTTAGAGGAATACGCAAACATAAATGTGTGGCAGATTAATCCCATTTCTTTTGACATCCGATTCATTGATGCCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009743 GO:0009756 GO:0009987 GO:0010033 GO:0010182 GO:0016043 GO:0023052 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051276 GO:0051716 GO:0065007 GO:0070887 GO:0071103 GO:0071310 GO:0071322 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

722

Amino Acids

81.14

Weight (kDa)

5.55

Isoelectric Point (pI)

47.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	11 - 126	1.9e-12	MCM N-terminal domain
MCM_OB	PF17207	145 - 276	1.6e-34	MCM OB domain
MCM	PF00493	316 - 538	2.4e-101	MCM P-loop domain
MCM_lid	PF17855	555 - 638	3.2e-20	MCM AAA-lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005152)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02060 AT4G02060
fragaria_vesca	FvH4_5g39230 FvH4_5g39230 FvH4_5g39230 FvH4_5g39230
malus_domestica	MD01G1012600.v1.1 MD03G1295400.v1.1 MD11G1312000.v1.1 MD11G1312600.v1.1 MD11G1314500.v1.1
prunus_persica	Prupe.8G272000_v2.0.a1
pyrus_communis	pycom01g04470
rosa_chinensis	RchiOBHm_Chr7g0241501
rosa_laevigata	RLG00000000575
rosa_multiflora	Rmu_co8153082.1_g000001 Rmu_sc0040921.1_g000002
rosa_roxburghii	Rroxscaffold_3G00219090 Rroxscaffold_3G00241100 Rroxscaffold_3G00241110 Rroxscaffold_3G00241120 Rroxscaffold_5G00346280
rosa_rugosa	Rorug03G0267600 Rorug07G0344400 Rorug07G0344500
rosa_samantha	Rh7AG498900 Rh7BG469600 Rh7CG515900 Rh7DG483700
rosa_wichuraiana	Rw7G042140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	307
Acc16I	TGCGCA	1 cut(s)	92
Acc36I	ACCTGC	1 cut(s)	511
AccB1I	GGYRCC	1 cut(s)	1009
AccII	CGCG	1 cut(s)	493
AccIII	TCCGGA	1 cut(s)	340
AciI	CCGC	5 cut(s)	115, 184, 493, 853, 1301
AclWI	GGATC	8 cut(s)	338, 351, 1130, 1247, 1418, 1566, 1952, 1965
AcsI	RAATTY	3 cut(s)	119, 179, 680
AcuI	CTGAAG	2 cut(s)	732, 1689
AfaI	GTAC	2 cut(s)	1357, 1928
AfeI	AGCGCT	2 cut(s)	22, 377
AfiI	CCNNNNNNNGG	4 cut(s)	836, 1407, 1466, 1637
AflIII	ACRYGT	1 cut(s)	536
AgsI	TTSAA	9 cut(s)	101, 197, 386, 408, 602, 656, 679, 1426, 1980
AjnI	CCWGG	6 cut(s)	564, 611, 1138, 1187, 1275, 1554
Alw21I	GWGCWC	1 cut(s)	36
Alw26I	GTCTC	2 cut(s)	1704, 1994
AlwI	GGATC	8 cut(s)	338, 351, 1130, 1247, 1418, 1566, 1952, 1965
Ama87I	CYCGRG	1 cut(s)	579
Aor13HI	TCCGGA	1 cut(s)	340
Aor51HI	AGCGCT	2 cut(s)	22, 377
AoxI	GGCC	2 cut(s)	727, 1518
ApeKI	GCWGC	5 cut(s)	1238, 1381, 1448, 1451, 1835
ApoI	RAATTY	3 cut(s)	119, 179, 680
AseI	ATTAAT	3 cut(s)	1176, 1506, 2124
Asp700I	GAANNNNTTC	1 cut(s)	717
AspLEI	GCGC	4 cut(s)	23, 93, 378, 1070
AspS9I	GGNCC	4 cut(s)	320, 338, 1273, 1519
AsuC2I	CCSGG	2 cut(s)	761, 789
AsuHPI	GGTGA	4 cut(s)	430, 776, 803, 1145
AsuII	TTCGAA	2 cut(s)	68, 1825
AsuNHI	GCTAGC	1 cut(s)	1297
AvaI	CYCGRG	1 cut(s)	579
AvaII	GGWCC	3 cut(s)	320, 338, 1273
BaeI	ACNNNNGTAYC	2 cut(s)	689, 722
BamHI	GGATCC	2 cut(s)	343, 1957
BanI	GGYRCC	1 cut(s)	1009
BanII	GRGCYC	2 cut(s)	36, 261
Bbv12I	GWGCWC	1 cut(s)	36
BbvCI	CCTCAGC	1 cut(s)	969
BbvI	GCAGC	5 cut(s)	1225, 1393, 1435, 1463, 1822
BciT130I	CCWGG	6 cut(s)	566, 613, 1140, 1189, 1277, 1556
BciVI	GTATCC	1 cut(s)	2054
BclI	TGATCA	1 cut(s)	283
BcnI	CCSGG	2 cut(s)	761, 789
BcoDI	GTCTC	2 cut(s)	1704, 1994
BfaI	CTAG	6 cut(s)	1061, 1298, 1593, 1737, 1749, 2052
BfmI	CTRYAG	2 cut(s)	1449, 1683
BfoI	RGCGCY	3 cut(s)	24, 379, 1071
BfuAI	ACCTGC	1 cut(s)	511
BfuI	GTATCC	1 cut(s)	2054
BisI	GCNGC	5 cut(s)	1239, 1382, 1449, 1452, 1836
BlpI	GCTNAGC	1 cut(s)	1814
BlsI	GCNGC	5 cut(s)	1240, 1383, 1450, 1453, 1837
Bme1390I	CCNGG	8 cut(s)	566, 613, 761, 789, 1140, 1189, 1277, 1556
Bme18I	GGWCC	3 cut(s)	320, 338, 1273
BmeT110I	CYCGRG	1 cut(s)	579
BmgT120I	GGNCC	4 cut(s)	320, 338, 1273, 1519
BmiI	GGNNCC	5 cut(s)	258, 345, 644, 1011, 1959
BmrFI	CCNGG	8 cut(s)	566, 613, 761, 789, 1140, 1189, 1277, 1556
BmsI	GCATC	8 cut(s)	102, 223, 348, 410, 507, 674, 1893, 2146
BmtI	GCTAGC	1 cut(s)	1301
BpmI	CTGGAG	1 cut(s)	1161
Bpu10I	CCTNAGC	1 cut(s)	969
Bpu1102I	GCTNAGC	1 cut(s)	1814
Bpu14I	TTCGAA	2 cut(s)	68, 1825
BpuEI	CTTGAG	3 cut(s)	1604, 1673, 1742
BpuMI	CCSGG	2 cut(s)	761, 789
BsaAI	YACGTR	1 cut(s)	1484
BsaBI	GATNNNNATC	2 cut(s)	282, 1578
BsaJI	CCNNGG	6 cut(s)	39, 737, 760, 1187, 1188, 1401
BsaWI	WCCGGW	1 cut(s)	340
BsaXI	ACNNNNNCTCC	2 cut(s)	1278, 1308
Bsc4I	CCNNNNNNNGG	4 cut(s)	836, 1407, 1466, 1637
Bse118I	RCCGGY	1 cut(s)	1516
Bse1I	ACTGG	2 cut(s)	841, 1210
Bse3DI	GCAATG	2 cut(s)	855, 1395
Bse8I	GATNNNNATC	2 cut(s)	282, 1578
BseAI	TCCGGA	1 cut(s)	340
BseBI	CCWGG	6 cut(s)	566, 613, 1140, 1189, 1277, 1556
BseDI	CCNNGG	6 cut(s)	39, 737, 760, 1187, 1188, 1401
BseGI	GGATG	9 cut(s)	93, 178, 340, 608, 979, 1348, 1891, 2023, 2142
BseJI	GATNNNNATC	2 cut(s)	282, 1578
BseLI	CCNNNNNNNGG	4 cut(s)	836, 1407, 1466, 1637
BseMI	GCAATG	2 cut(s)	855, 1395
BseMII	CTCAG	5 cut(s)	673, 960, 1828, 1944, 1989
BseNI	ACTGG	2 cut(s)	841, 1210
BseRI	GAGGAG	4 cut(s)	1062, 1122, 1299, 1739
BseXI	GCAGC	5 cut(s)	1225, 1393, 1435, 1463, 1822
BseYI	CCCAGC	1 cut(s)	1406
Bsh1236I	CGCG	1 cut(s)	493
BshFI	GGCC	2 cut(s)	729, 1520
BshNI	GGYRCC	1 cut(s)	1009
BsiHKAI	GWGCWC	1 cut(s)	36
BsiHKCI	CYCGRG	1 cut(s)	579
BsiSI	CCGG	6 cut(s)	254, 341, 760, 788, 1517, 1631
BslFI	GGGAC	1 cut(s)	1694
BslI	CCNNNNNNNGG	4 cut(s)	836, 1407, 1466, 1637
BsmAI	GTCTC	2 cut(s)	1704, 1994
BsmBI	CGTCTC	1 cut(s)	1994
BsmFI	GGGAC	1 cut(s)	1694
BsmI	GAATGC	5 cut(s)	608, 1432, 1752, 1783, 1818
BsnI	GGCC	2 cut(s)	729, 1520
BsoBI	CYCGRG	1 cut(s)	579
Bsp119I	TTCGAA	2 cut(s)	68, 1825
Bsp1286I	GDGCHC	2 cut(s)	36, 261
Bsp13I	TCCGGA	1 cut(s)	340
Bsp1720I	GCTNAGC	1 cut(s)	1814
BspACI	CCGC	5 cut(s)	115, 184, 493, 853, 1301
BspANI	GGCC	2 cut(s)	729, 1520
BspCNI	CTCAG	5 cut(s)	672, 961, 1827, 1943, 1990
BspEI	TCCGGA	1 cut(s)	340
BspFNI	CGCG	1 cut(s)	493
BspHI	TCATGA	2 cut(s)	286, 297
BspLI	GGNNCC	5 cut(s)	258, 345, 644, 1011, 1959
BspMAI	CTGCAG	2 cut(s)	1453, 1687
BspMI	ACCTGC	1 cut(s)	511
BspOI	GCTAGC	1 cut(s)	1301
BspPI	GGATC	8 cut(s)	338, 351, 1130, 1247, 1418, 1566, 1952, 1965
BspT104I	TTCGAA	2 cut(s)	68, 1825
BspT107I	GGYRCC	1 cut(s)	1009
BsrDI	GCAATG	2 cut(s)	855, 1395
BsrFI	RCCGGY	1 cut(s)	1516
BsrI	ACTGG	2 cut(s)	841, 1210
BssAI	RCCGGY	1 cut(s)	1516
BssECI	CCNNGG	6 cut(s)	39, 737, 760, 1187, 1188, 1401
BssT1I	CCWWGG	2 cut(s)	39, 1401
Bst2UI	CCWGG	6 cut(s)	566, 613, 1140, 1189, 1277, 1556
Bst4CI	ACNGT	5 cut(s)	751, 1390, 1801, 1867, 2066
BstAPI	GCANNNNNTGC	1 cut(s)	1436
BstBAI	YACGTR	1 cut(s)	1484
BstBI	TTCGAA	2 cut(s)	68, 1825
BstC8I	GCNNGC	7 cut(s)	36, 855, 859, 996, 1157, 1299, 2012
BstDEI	CTNAG	8 cut(s)	659, 862, 880, 906, 969, 1814, 1930, 1998
BstDSI	CCRYGG	1 cut(s)	737
BstF5I	GGATG	9 cut(s)	93, 178, 340, 608, 979, 1348, 1891, 2023, 2142
BstFNI	CGCG	1 cut(s)	493
BstH2I	RGCGCY	3 cut(s)	24, 379, 1071
BstHHI	GCGC	4 cut(s)	23, 93, 378, 1070
BstMAI	GTCTC	2 cut(s)	1704, 1994
BstMWI	GCNNNNNNNGC	8 cut(s)	517, 854, 965, 1215, 1436, 1445, 1460, 1841
BstNI	CCWGG	6 cut(s)	566, 613, 1140, 1189, 1277, 1556
BstNSI	RCATGY	4 cut(s)	91, 540, 1601, 2036
BstSCI	CCNGG	8 cut(s)	564, 611, 759, 787, 1138, 1187, 1275, 1554
BstSFI	CTRYAG	2 cut(s)	1449, 1683
BstSNI	TACGTA	1 cut(s)	1484
BstUI	CGCG	1 cut(s)	493
BstV1I	GCAGC	5 cut(s)	1225, 1393, 1435, 1463, 1822
BstX2I	RGATCY	3 cut(s)	343, 1252, 1957
BstXI	CCANNNNNNTGG	1 cut(s)	1949
BstYI	RGATCY	3 cut(s)	343, 1252, 1957
BsuI	GTATCC	1 cut(s)	2054
BsuRI	GGCC	2 cut(s)	729, 1520
BtgI	CCRYGG	1 cut(s)	737
BtsCI	GGATG	9 cut(s)	93, 178, 340, 608, 979, 1348, 1891, 2023, 2142
BtsI	GCAGTG	2 cut(s)	1225, 1692
BtsIMutI	CAGTG	3 cut(s)	1225, 1692, 2071
BveI	ACCTGC	1 cut(s)	511
Cac8I	GCNNGC	7 cut(s)	36, 855, 859, 996, 1157, 1299, 2012
CciI	TCATGA	2 cut(s)	286, 297
CfoI	GCGC	4 cut(s)	23, 93, 378, 1070
Cfr10I	RCCGGY	1 cut(s)	1516
Cfr13I	GGNCC	4 cut(s)	320, 338, 1273, 1519
CseI	GACGC	1 cut(s)	499
Csp6I	GTAC	2 cut(s)	1356, 1927
CviQI	GTAC	2 cut(s)	1356, 1927
DdeI	CTNAG	8 cut(s)	659, 862, 880, 906, 969, 1814, 1930, 1998
DrdI	GACNNNNNNGTC	1 cut(s)	307
DseDI	GACNNNNNNGTC	1 cut(s)	307
Ecl136II	GAGCTC	1 cut(s)	34
Eco105I	TACGTA	1 cut(s)	1484
Eco130I	CCWWGG	2 cut(s)	39, 1401
Eco24I	GRGCYC	2 cut(s)	36, 261
Eco32I	GATATC	3 cut(s)	477, 1036, 1972
Eco47I	GGWCC	3 cut(s)	320, 338, 1273
Eco47III	AGCGCT	2 cut(s)	22, 377
Eco53kI	GAGCTC	1 cut(s)	34
Eco57I	CTGAAG	2 cut(s)	732, 1689
Eco88I	CYCGRG	1 cut(s)	579
EcoICRI	GAGCTC	1 cut(s)	34
EcoRI	GAATTC	1 cut(s)	179
EcoRII	CCWGG	6 cut(s)	564, 611, 1138, 1187, 1275, 1554
EcoRV	GATATC	3 cut(s)	477, 1036, 1972
EcoT14I	CCWWGG	2 cut(s)	39, 1401
EcoT22I	ATGCAT	1 cut(s)	1908
EcoT38I	GRGCYC	2 cut(s)	36, 261
ErhI	CCWWGG	2 cut(s)	39, 1401
Esp3I	CGTCTC	1 cut(s)	1994
FalI	AAGNNNNNCTT	4 cut(s)	365, 397, 388, 420
FaqI	GGGAC	1 cut(s)	1694
FauI	CCCGC	1 cut(s)	846
FauNDI	CATATG	2 cut(s)	427, 1908
FbaI	TGATCA	1 cut(s)	283
Fnu4HI	GCNGC	5 cut(s)	1239, 1382, 1449, 1452, 1836
FokI	GGATG	9 cut(s)	80, 185, 347, 595, 986, 1355, 1898, 2030, 2129
FriOI	GRGCYC	2 cut(s)	36, 261
Fsp4HI	GCNGC	5 cut(s)	1239, 1382, 1449, 1452, 1836
FspAI	RTGCGCAY	1 cut(s)	92
FspBI	CTAG	6 cut(s)	1061, 1298, 1593, 1737, 1749, 2052
FspI	TGCGCA	1 cut(s)	92
GlaI	GCGC	4 cut(s)	22, 92, 377, 1069
GluI	GCNGC	5 cut(s)	1239, 1382, 1449, 1452, 1836
GsaI	CCCAGC	1 cut(s)	1410
GsuI	CTGGAG	1 cut(s)	1161
HaeII	RGCGCY	3 cut(s)	24, 379, 1071
HaeIII	GGCC	2 cut(s)	729, 1520
HapII	CCGG	6 cut(s)	254, 341, 760, 788, 1517, 1631
HgaI	GACGC	1 cut(s)	499
HhaI	GCGC	4 cut(s)	23, 93, 378, 1070
Hin6I	GCGC	4 cut(s)	21, 91, 376, 1068
HinP1I	GCGC	4 cut(s)	21, 91, 376, 1068
HinfI	GANTC	7 cut(s)	65, 301, 582, 1625, 1848, 1994, 2148
HpaII	CCGG	6 cut(s)	254, 341, 760, 788, 1517, 1631
HphI	GGTGA	4 cut(s)	430, 776, 803, 1145
Hpy166II	GTNNAC	3 cut(s)	391, 754, 767
Hpy8I	GTNNAC	3 cut(s)	391, 754, 767
Hpy99I	CGWCG	1 cut(s)	2008
HpyAV	CCTTC	4 cut(s)	107, 1274, 1713, 2050
HpyCH4III	ACNGT	5 cut(s)	751, 1390, 1801, 1867, 2066
HpyCH4IV	ACGT	3 cut(s)	961, 1418, 1483
HpyF10VI	GCNNNNNNNGC	8 cut(s)	517, 854, 965, 1215, 1436, 1445, 1460, 1841
HpyF3I	CTNAG	8 cut(s)	659, 862, 880, 906, 969, 1814, 1930, 1998
HpySE526I	ACGT	3 cut(s)	961, 1418, 1483
HspAI	GCGC	4 cut(s)	21, 91, 376, 1068
Kpn2I	TCCGGA	1 cut(s)	340
Ksp22I	TGATCA	1 cut(s)	283
LmnI	GCTCC	6 cut(s)	256, 790, 1062, 1075, 1286, 1378
Lsp1109I	GCAGC	5 cut(s)	1225, 1393, 1435, 1463, 1822
LweI	GCATC	8 cut(s)	102, 223, 348, 410, 507, 674, 1893, 2146
MaeI	CTAG	6 cut(s)	1061, 1298, 1593, 1737, 1749, 2052
MaeII	ACGT	3 cut(s)	961, 1418, 1483
MaeIII	GTNAC	5 cut(s)	158, 487, 571, 977, 1258
MflI	RGATCY	3 cut(s)	343, 1252, 1957
MhlI	GDGCHC	2 cut(s)	36, 261
MluCI	AATT	8 cut(s)	119, 167, 179, 432, 680, 1173, 1346, 1980
MlyI	GAGTC	4 cut(s)	295, 591, 1842, 2003
MmeI	TCCRAC	2 cut(s)	78, 1876
Mph1103I	ATGCAT	1 cut(s)	1908
MroI	TCCGGA	1 cut(s)	340
MroXI	GAANNNNTTC	1 cut(s)	717
MseI	TTAA	6 cut(s)	507, 1176, 1506, 1550, 1895, 2124
MslI	CAYNNNNRTG	2 cut(s)	296, 2111
MspA1I	CMGCKG	1 cut(s)	1835
MspI	CCGG	6 cut(s)	254, 341, 760, 788, 1517, 1631
MspR9I	CCNGG	8 cut(s)	566, 613, 761, 789, 1140, 1189, 1277, 1556
Mva1269I	GAATGC	5 cut(s)	608, 1432, 1752, 1783, 1818
MvaI	CCWGG	6 cut(s)	566, 613, 1140, 1189, 1277, 1556
MvnI	CGCG	1 cut(s)	493
MwoI	GCNNNNNNNGC	8 cut(s)	517, 854, 965, 1215, 1436, 1445, 1460, 1841
NciI	CCSGG	2 cut(s)	761, 789
NdeI	CATATG	2 cut(s)	427, 1908
NheI	GCTAGC	1 cut(s)	1297
NlaIV	GGNNCC	5 cut(s)	258, 345, 644, 1011, 1959
NmeAIII	GCCGAG	1 cut(s)	187
NmuCI	GTSAC	2 cut(s)	487, 1258
NsbI	TGCGCA	1 cut(s)	92
NsiI	ATGCAT	1 cut(s)	1908
NspI	RCATGY	4 cut(s)	91, 540, 1601, 2036
NspV	TTCGAA	2 cut(s)	68, 1825
PaeR7I	CTCGAG	1 cut(s)	579
PagI	TCATGA	2 cut(s)	286, 297
PasI	CCCWGGG	1 cut(s)	1188
PciI	ACATGT	1 cut(s)	536
PctI	GAATGC	5 cut(s)	608, 1432, 1752, 1783, 1818
PdmI	GAANNNNTTC	1 cut(s)	717
PfeI	GAWTC	3 cut(s)	65, 1625, 2148
PfoI	TCCNGGA	3 cut(s)	1138, 1275, 1554
PkrI	GCNGC	5 cut(s)	1240, 1383, 1450, 1453, 1837
PleI	GAGTC	4 cut(s)	295, 590, 1842, 2002
PpsI	GAGTC	4 cut(s)	295, 590, 1842, 2002
Ppu21I	YACGTR	1 cut(s)	1484
PscI	ACATGT	1 cut(s)	536
PshBI	ATTAAT	3 cut(s)	1176, 1506, 2124
Psp124BI	GAGCTC	1 cut(s)	36
Psp6I	CCWGG	6 cut(s)	564, 611, 1138, 1187, 1275, 1554
PspFI	CCCAGC	1 cut(s)	1406
PspGI	CCWGG	6 cut(s)	564, 611, 1138, 1187, 1275, 1554
PspN4I	GGNNCC	5 cut(s)	258, 345, 644, 1011, 1959
PspPI	GGNCC	4 cut(s)	320, 338, 1273, 1519
PspXI	VCTCGAGB	1 cut(s)	579
PstI	CTGCAG	2 cut(s)	1453, 1687
PsuI	RGATCY	3 cut(s)	343, 1252, 1957
PvuII	CAGCTG	1 cut(s)	1835
RsaI	GTAC	2 cut(s)	1357, 1928
RsaNI	GTAC	2 cut(s)	1356, 1927
RseI	CAYNNNNRTG	2 cut(s)	296, 2111
SacI	GAGCTC	1 cut(s)	36
SaqAI	TTAA	6 cut(s)	507, 1176, 1506, 1550, 1895, 2124
SatI	GCNGC	5 cut(s)	1239, 1382, 1449, 1452, 1836
Sau96I	GGNCC	4 cut(s)	320, 338, 1273, 1519
SchI	GAGTC	4 cut(s)	295, 591, 1842, 2003
ScrFI	CCNGG	8 cut(s)	566, 613, 761, 789, 1140, 1189, 1277, 1556
SduI	GDGCHC	2 cut(s)	36, 261
SfaNI	GCATC	8 cut(s)	102, 223, 348, 410, 507, 674, 1893, 2146
SfcI	CTRYAG	2 cut(s)	1449, 1683
Sfr274I	CTCGAG	1 cut(s)	579
SfuI	TTCGAA	2 cut(s)	68, 1825
SinI	GGWCC	3 cut(s)	320, 338, 1273
SlaI	CTCGAG	1 cut(s)	579
SmiMI	CAYNNNNRTG	2 cut(s)	296, 2111
SmlI	CTYRAG	4 cut(s)	579, 1583, 1652, 1721
SmoI	CTYRAG	4 cut(s)	579, 1583, 1652, 1721
SnaBI	TACGTA	1 cut(s)	1484
Sse9I	AATT	8 cut(s)	119, 167, 179, 432, 680, 1173, 1346, 1980
SsiI	CCGC	5 cut(s)	115, 184, 493, 853, 1301
SspMI	CTAG	6 cut(s)	1061, 1298, 1593, 1737, 1749, 2052
SstI	GAGCTC	1 cut(s)	36
StyD4I	CCNGG	8 cut(s)	564, 611, 759, 787, 1138, 1187, 1275, 1554
StyI	CCWWGG	2 cut(s)	39, 1401
TaaI	ACNGT	5 cut(s)	751, 1390, 1801, 1867, 2066
TaiI	ACGT	3 cut(s)	964, 1421, 1486
TasI	AATT	8 cut(s)	119, 167, 179, 432, 680, 1173, 1346, 1980
TatI	WGTACW	1 cut(s)	1926
TfiI	GAWTC	3 cut(s)	65, 1625, 2148
Tru1I	TTAA	6 cut(s)	507, 1176, 1506, 1550, 1895, 2124
Tru9I	TTAA	6 cut(s)	507, 1176, 1506, 1550, 1895, 2124
TscAI	CASTG	3 cut(s)	1225, 1692, 2071
TseFI	GTSAC	2 cut(s)	487, 1258
TseI	GCWGC	5 cut(s)	1238, 1381, 1448, 1451, 1835
Tsp45I	GTSAC	2 cut(s)	487, 1258
TspGWI	ACGGA	1 cut(s)	754
TspRI	CASTG	3 cut(s)	1225, 1692, 2071
VpaK11BI	GGWCC	3 cut(s)	320, 338, 1273
VspI	ATTAAT	3 cut(s)	1176, 1506, 2124
XapI	RAATTY	3 cut(s)	119, 179, 680
XceI	RCATGY	4 cut(s)	91, 540, 1601, 2036
XhoI	CTCGAG	1 cut(s)	579
XmnI	GAANNNNTTC	1 cut(s)	717
XspI	CTAG	6 cut(s)	1061, 1298, 1593, 1737, 1749, 2052
Zsp2I	ATGCAT	1 cut(s)	1908

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.