Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0005152

Physical Location & Seq

Genomic Coordinates Reverse (-)

1270646 .. 1276411

Gene Structure 5766 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00219090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2157 bp

ATGAAGGACCTCAACTTCGACGCCGACAAAGCGCTAGCGAAGGAATTCCTCTCCAACTTTGCCGATGCGGTTGGGGAACCCAAATACATGAACATCCTTCAAGAAGTTGCAAATCGCAAAATTCGGGCAATCCAGATTGATCTCGAGGACTTGTTTAATTACCAGGATTTCGACGAAGAATTCCTCAGACGGGTTACTGAAAACACCCGGCGGTATGTGGGTATTTTTGCTGATGCTATTGATGAGCTCATGCCGCAGCCCACTGAGGCCTTTACAGATGATGACCATGACATCTTGATGACACAAAGGTCTGATGATGGACCCGAGAATATGGATGGTTCTGATCCACATCAGAAGATGCCTCCGGAAATCAAGCGCTACTTTGAAGTTTACATAAGAGCATCTTCAAAGGGGCAGCCATTTACCATCAGGGAGGTGAAGGCTTCATATATTGGTCAGCTTGTGAGGATATCAGGTATTGTGACGCGGTGTTCGGATGTTAAGCCATTGATGCAGGTTGCTGTGTACACGTGTGAAGAATGCGGTTTCGAGATTTACCAGGAAGTAACTGCTCGAATCTTTATGCCTCTGTTCGAATGCCCATCTAGGCGTTGCAATATTAACAAAACAAAGGGGAACCTTATTCTTCAGCTCAGGGCATCAAAGTTTTTGAAGTTTCAGGAGGCCAAGATACAAGAGTTGGCTGAACATGTTCCAAAAGGCCATATTCCTCGGACAATGACTGTTCACTTCAGGGGTGAACTTACAAGAAAGGTAGCTCCAGGTGATGTTGTTGAATTATCTGGCATATTTCTCCCCATTCCATACACTGGTTTTAGAGCAATGCGAGCTGGCTTAGTTGCAGATACGTACTTAGAAGCCATGTCTGTTGCACATTACAAGAAAAAATATGAAGAGTATGAACTTAGAGCAGATGAGGAAGAACAAATTAGAAGTTTAGCTGATGATGGGGACATTTACAATAAGTTGGCACGATCATTGGCACCTGAAATTTATGGTCATGAAGATATAAAGAAGGCTCTGCTTCTGCTCCTAGTGGGTGCCCCTCACAGGAAACTGAAGGATGGAATGAAGATTAGAGGAGATTTACATATCTGTTTAATGGGTGATCCTGGAGTTGCAAAAAGTCAGCTTCTCAAACACATAATTAATGTAGCACCCAGGGGAGTTTATACAACTGGTAAAGGAAGTAGTGGAGTTGGTCTGACTGCTGCTGTTCAGAAAGATCAGGTCACAAATGAAATGGTCTTGGAAGGAGGAGCATTGGTGCTAGCTGATATGGGTATTTGTGCTATCGATGAGTTTGACAAGATGGATGAATTAGATCGTACAGCAATACATGAAGTTATGGAGCAGCAAACCGTCAGCATTGCTAAGGCTGGGATAACTACATCTTTGAATGCAAGAACTGCTGTTCTTGCTGCAGCTAATCCAGCATGGGGGAGATATGACCTACGTAGAACTCCAGCTGAAAACATTAATCTTCCTCCAGCCCTTTTGTCAAGATTTGATCTTCTCTGGTTGATCCTGGATCGAGCAGATATGGATAGTGATCTTGAGATGGCTAGGCATATTGTTTATGTCCACCAGAATAAAGAATCTCCGGCTCTGGGCTTCACTCCACTTGAACCATCTGTTCTTCGCGCATATATTTCTGCTGCAAGAAGATTGTCTCCTTCTGTCCCACCGGAACTTGAGGAGTACATTGCAAGTGCCTACTCTGCAATTCGGCAAGAAGAAGCTAAATCTAATGCTCCCCACTCCTATACAACTGTGAGAACTCTGCTCAGCATTCTTCGCATATCAGCTGCACTAGCAAGACTCCGATTCTCTGAAACAGTTGCTCAGAGTGACGTGGATGAGTCGCTCAGGCTAATGCAGATGTCCAAGTTCTCTTTATACTCAGACGATCGTCAGAGATCTGGACTTGATGCCATCTCTGATATCTATTCAATTCTGCGAGATGAAGCTGCCAGGACAAATAAGCTAGATGTGAGCTATGGACATGCACTGAATTGGATTTCTAGAAAGGGTTACAGTGAAGCCCAACTGAAAGAATGTTTGGAGGAATACGCAGCCTTGAATGTGTGGCAGATTAATCCCCAAACTTTTGACATCAGATTCATCGACGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009743 GO:0009756 GO:0009987 GO:0010033 GO:0010182 GO:0016043 GO:0023052 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051276 GO:0051716 GO:0065007 GO:0070887 GO:0071103 GO:0071310 GO:0071322 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

718

Amino Acids

80.95

Weight (kDa)

5.52

Isoelectric Point (pI)

47.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	13 - 126	1.1e-11	MCM N-terminal domain
MCM_OB	PF17207	143 - 274	1.9e-34	MCM OB domain
MCM	PF00493	314 - 536	9.5e-102	MCM P-loop domain
MCM_lid	PF17855	553 - 636	1.3e-21	MCM AAA-lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005152)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02060 AT4G02060
fragaria_vesca	FvH4_5g39230 FvH4_5g39230 FvH4_5g39230 FvH4_5g39230
malus_domestica	MD01G1012600.v1.1 MD03G1295400.v1.1 MD11G1312000.v1.1 MD11G1312600.v1.1 MD11G1314500.v1.1
prunus_persica	Prupe.8G272000_v2.0.a1
pyrus_communis	pycom01g04470
rosa_chinensis	RchiOBHm_Chr7g0241501
rosa_laevigata	RLG00000000575
rosa_multiflora	Rmu_co8153082.1_g000001 Rmu_sc0040921.1_g000002
rosa_roxburghii	Rroxscaffold_3G00219090 Rroxscaffold_3G00241100 Rroxscaffold_3G00241110 Rroxscaffold_3G00241120 Rroxscaffold_5G00346280
rosa_rugosa	Rorug03G0267600 Rorug07G0344400 Rorug07G0344500
rosa_samantha	Rh7AG498900 Rh7BG469600 Rh7CG515900 Rh7DG483700
rosa_wichuraiana	Rw7G042140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	307
Acc36I	ACCTGC	1 cut(s)	505
AccB1I	GGYRCC	2 cut(s)	1003, 1061
AccB7I	CCANNNNNTGG	1 cut(s)	830
AccII	CGCG	2 cut(s)	487, 1665
AccIII	TCCGGA	1 cut(s)	364
AciI	CCGC	5 cut(s)	68, 211, 254, 487, 543
AclWI	GGATC	4 cut(s)	338, 1124, 1540, 1560
AcsI	RAATTY	4 cut(s)	44, 120, 179, 1011
AcuI	CTGAAG	3 cut(s)	632, 736, 1100
AcvI	CACGTG	1 cut(s)	531
AcyI	GRCGYC	2 cut(s)	21, 2151
AfaI	GTAC	4 cut(s)	527, 872, 1351, 1724
AfeI	AGCGCT	2 cut(s)	33, 377
AfiI	CCNNNNNNNGG	5 cut(s)	190, 830, 1071, 1460, 1631
AflIII	ACRYGT	3 cut(s)	528, 530, 709
AgsI	TTSAA	9 cut(s)	101, 386, 408, 673, 797, 1420, 1649, 1974, 2104
AjiI	CACGTC	1 cut(s)	1876
AjnI	CCWGG	7 cut(s)	162, 558, 781, 1132, 1181, 1548, 1994
AjuI	GAANNNNNNNTTGG	2 cut(s)	2066, 2098
Alw21I	GWGCWC	1 cut(s)	249
Alw26I	GTCTC	1 cut(s)	1698
AlwI	GGATC	4 cut(s)	338, 1124, 1540, 1560
Ama87I	CYCGRG	2 cut(s)	143, 323
Aor13HI	TCCGGA	1 cut(s)	364
Aor51HI	AGCGCT	2 cut(s)	33, 377
AoxI	GGCC	3 cut(s)	267, 684, 721
ApoI	RAATTY	4 cut(s)	44, 120, 179, 1011
ArsI	GACNNNNNNTTYG	3 cut(s)	31, 475, 507
AseI	ATTAAT	3 cut(s)	1170, 1500, 2118
Asp700I	GAANNNNTTC	2 cut(s)	44, 711
AspLEI	GCGC	3 cut(s)	34, 378, 1667
AspS9I	GGNCC	2 cut(s)	7, 320
AsuC2I	CCSGG	1 cut(s)	208
AsuHPI	GGTGA	4 cut(s)	448, 770, 797, 1139
AsuII	TTCGAA	1 cut(s)	594
AsuNHI	GCTAGC	2 cut(s)	34, 1291
AvaI	CYCGRG	2 cut(s)	143, 323
AvaII	GGWCC	2 cut(s)	7, 320
BaeGI	GKGCMC	1 cut(s)	1066
BaeI	ACNNNNGTAYC	2 cut(s)	683, 716
BanI	GGYRCC	2 cut(s)	1003, 1061
BanII	GRGCYC	1 cut(s)	249
BbrPI	CACGTG	1 cut(s)	531
Bbv12I	GWGCWC	1 cut(s)	249
BciT130I	CCWGG	7 cut(s)	164, 560, 783, 1134, 1183, 1550, 1996
BcnI	CCSGG	1 cut(s)	208
BcoDI	GTCTC	1 cut(s)	1698
BfaI	CTAG	8 cut(s)	35, 606, 1055, 1292, 1587, 1835, 2009, 2046
BfmI	CTRYAG	1 cut(s)	1443
BfoI	RGCGCY	2 cut(s)	35, 379
BfuAI	ACCTGC	1 cut(s)	505
BglII	AGATCT	1 cut(s)	1940
BlpI	GCTNAGC	1 cut(s)	1808
Bme1390I	CCNGG	8 cut(s)	164, 208, 560, 783, 1134, 1183, 1550, 1996
Bme18I	GGWCC	2 cut(s)	7, 320
BmeT110I	CYCGRG	2 cut(s)	143, 323
BmgBI	CACGTC	1 cut(s)	1876
BmgT120I	GGNCC	2 cut(s)	7, 320
BmiI	GGNNCC	5 cut(s)	78, 322, 638, 1005, 1063
BmrFI	CCNGG	8 cut(s)	164, 208, 560, 783, 1134, 1183, 1550, 1996
BmsI	GCATC	7 cut(s)	55, 223, 348, 410, 501, 668, 1942
BmtI	GCTAGC	2 cut(s)	38, 1295
BoxI	GACNNNNGTC	1 cut(s)	1932
BpmI	CTGGAG	4 cut(s)	765, 1155, 1470, 1494
Bpu10I	CCTNAGC	3 cut(s)	653, 1395, 1889
Bpu1102I	GCTNAGC	1 cut(s)	1808
Bpu14I	TTCGAA	1 cut(s)	594
BpuEI	CTTGAG	2 cut(s)	1598, 1736
BpuMI	CCSGG	1 cut(s)	208
Bsa29I	ATCGAT	1 cut(s)	1317
BsaAI	YACGTR	3 cut(s)	531, 870, 1478
BsaBI	GATNNNNATC	2 cut(s)	348, 1572
BsaHI	GRCGYC	2 cut(s)	21, 2151
BsaJI	CCNNGG	3 cut(s)	731, 1181, 1182
BsaWI	WCCGGW	2 cut(s)	364, 1708
BsaXI	ACNNNNNCTCC	2 cut(s)	1272, 1302
Bsc4I	CCNNNNNNNGG	5 cut(s)	190, 830, 1071, 1460, 1631
Bse1I	ACTGG	2 cut(s)	835, 1204
Bse3DI	GCAATG	3 cut(s)	849, 1389, 1725
Bse8I	GATNNNNATC	2 cut(s)	348, 1572
BseAI	TCCGGA	1 cut(s)	364
BseBI	CCWGG	7 cut(s)	164, 560, 783, 1134, 1183, 1550, 1996
BseCI	ATCGAT	1 cut(s)	1317
BseDI	CCNNGG	3 cut(s)	731, 1181, 1182
BseGI	GGATG	6 cut(s)	93, 340, 502, 1090, 1342, 1885
BseJI	GATNNNNATC	2 cut(s)	348, 1572
BseLI	CCNNNNNNNGG	5 cut(s)	190, 830, 1071, 1460, 1631
BseMI	GCAATG	3 cut(s)	849, 1389, 1725
BseMII	CTCAG	7 cut(s)	199, 255, 667, 1822, 1880, 1903, 1938
BseNI	ACTGG	2 cut(s)	835, 1204
BseRI	GAGGAG	3 cut(s)	1116, 1293, 1733
BseSI	GKGCMC	1 cut(s)	1066
BseYI	CCCAGC	1 cut(s)	1400
BsgI	GTGCAG	1 cut(s)	1815
Bsh1236I	CGCG	2 cut(s)	487, 1665
Bsh1285I	CGRYCG	1 cut(s)	1933
BshFI	GGCC	3 cut(s)	269, 686, 723
BshNI	GGYRCC	2 cut(s)	1003, 1061
BshVI	ATCGAT	1 cut(s)	1317
BsiEI	CGRYCG	1 cut(s)	1933
BsiHKAI	GWGCWC	1 cut(s)	249
BsiHKCI	CYCGRG	2 cut(s)	143, 323
BsiSI	CCGG	4 cut(s)	208, 365, 1625, 1709
BslFI	GGGAC	2 cut(s)	986, 1688
BslI	CCNNNNNNNGG	5 cut(s)	190, 830, 1071, 1460, 1631
BsmAI	GTCTC	1 cut(s)	1698
BsmFI	GGGAC	2 cut(s)	986, 1688
BsmI	GAATGC	4 cut(s)	545, 602, 1426, 1812
BsnI	GGCC	3 cut(s)	269, 686, 723
BsoBI	CYCGRG	2 cut(s)	143, 323
Bsp119I	TTCGAA	1 cut(s)	594
Bsp1286I	GDGCHC	2 cut(s)	249, 1066
Bsp13I	TCCGGA	1 cut(s)	364
Bsp1407I	TGTACA	1 cut(s)	525
Bsp1720I	GCTNAGC	1 cut(s)	1808
BspACI	CCGC	5 cut(s)	68, 211, 254, 487, 543
BspANI	GGCC	3 cut(s)	269, 686, 723
BspCNI	CTCAG	7 cut(s)	198, 256, 666, 1821, 1879, 1902, 1937
BspDI	ATCGAT	1 cut(s)	1317
BspEI	TCCGGA	1 cut(s)	364
BspFNI	CGCG	2 cut(s)	487, 1665
BspHI	TCATGA	1 cut(s)	1021
BspLI	GGNNCC	5 cut(s)	78, 322, 638, 1005, 1063
BspMAI	CTGCAG	1 cut(s)	1447
BspMI	ACCTGC	1 cut(s)	505
BspOI	GCTAGC	2 cut(s)	38, 1295
BspPI	GGATC	4 cut(s)	338, 1124, 1540, 1560
BspT104I	TTCGAA	1 cut(s)	594
BspT107I	GGYRCC	2 cut(s)	1003, 1061
BsrDI	GCAATG	3 cut(s)	849, 1389, 1725
BsrGI	TGTACA	1 cut(s)	525
BsrI	ACTGG	2 cut(s)	835, 1204
BssECI	CCNNGG	3 cut(s)	731, 1181, 1182
BssNI	GRCGYC	2 cut(s)	21, 2151
Bst2UI	CCWGG	7 cut(s)	164, 560, 783, 1134, 1183, 1550, 1996
Bst4CI	ACNGT	5 cut(s)	745, 1384, 1795, 1861, 2060
Bst6I	CTCTTC	1 cut(s)	909
BstACI	GRCGYC	2 cut(s)	21, 2151
BstAPI	GCANNNNNTGC	1 cut(s)	1430
BstAUI	TGTACA	1 cut(s)	525
BstBAI	YACGTR	3 cut(s)	531, 870, 1478
BstBI	TTCGAA	1 cut(s)	594
BstC8I	GCNNGC	4 cut(s)	36, 849, 853, 1293
BstF5I	GGATG	6 cut(s)	93, 340, 502, 1090, 1342, 1885
BstFNI	CGCG	2 cut(s)	487, 1665
BstH2I	RGCGCY	2 cut(s)	35, 379
BstHHI	GCGC	3 cut(s)	34, 378, 1667
BstMAI	GTCTC	1 cut(s)	1698
BstMCI	CGRYCG	1 cut(s)	1933
BstNI	CCWGG	7 cut(s)	164, 560, 783, 1134, 1183, 1550, 1996
BstNSI	RCATGY	2 cut(s)	713, 2030
BstPAI	GACNNNNGTC	1 cut(s)	1932
BstSCI	CCNGG	8 cut(s)	162, 206, 558, 781, 1132, 1181, 1548, 1994
BstSFI	CTRYAG	1 cut(s)	1443
BstSLI	GKGCMC	1 cut(s)	1066
BstSNI	TACGTA	2 cut(s)	870, 1478
BstUI	CGCG	2 cut(s)	487, 1665
BstX2I	RGATCY	1 cut(s)	1940
BstYI	RGATCY	1 cut(s)	1940
Bsu15I	ATCGAT	1 cut(s)	1317
BsuRI	GGCC	3 cut(s)	269, 686, 723
BsuTUI	ATCGAT	1 cut(s)	1317
BtrI	CACGTC	1 cut(s)	1876
BtsCI	GGATG	6 cut(s)	93, 340, 502, 1090, 1342, 1885
BtsIMutI	CAGTG	4 cut(s)	261, 828, 2030, 2065
BveI	ACCTGC	1 cut(s)	505
Cac8I	GCNNGC	4 cut(s)	36, 849, 853, 1293
CciI	TCATGA	1 cut(s)	1021
CfoI	GCGC	3 cut(s)	34, 378, 1667
Cfr13I	GGNCC	2 cut(s)	7, 320
ClaI	ATCGAT	1 cut(s)	1317
CseI	GACGC	2 cut(s)	29, 493
Csp6I	GTAC	4 cut(s)	526, 871, 1350, 1723
CviAII	CATG	9 cut(s)	88, 250, 287, 710, 883, 1022, 1361, 1458, 2027
CviQI	GTAC	4 cut(s)	526, 871, 1350, 1723
DrdI	GACNNNNNNGTC	1 cut(s)	307
DseDI	GACNNNNNNGTC	1 cut(s)	307
Eam1104I	CTCTTC	1 cut(s)	909
EarI	CTCTTC	1 cut(s)	909
Ecl136II	GAGCTC	1 cut(s)	247
Eco105I	TACGTA	2 cut(s)	870, 1478
Eco147I	AGGCCT	1 cut(s)	269
Eco24I	GRGCYC	1 cut(s)	249
Eco32I	GATATC	2 cut(s)	471, 1966
Eco47I	GGWCC	2 cut(s)	7, 320
Eco47III	AGCGCT	2 cut(s)	33, 377
Eco53kI	GAGCTC	1 cut(s)	247
Eco57I	CTGAAG	3 cut(s)	632, 736, 1100
Eco72I	CACGTG	1 cut(s)	531
Eco88I	CYCGRG	2 cut(s)	143, 323
EcoICRI	GAGCTC	1 cut(s)	247
EcoO109I	RGGNCCY	1 cut(s)	7
EcoRI	GAATTC	2 cut(s)	44, 179
EcoRII	CCWGG	7 cut(s)	162, 558, 781, 1132, 1181, 1548, 1994
EcoRV	GATATC	2 cut(s)	471, 1966
EcoT38I	GRGCYC	1 cut(s)	249
FaeI	CATG	9 cut(s)	91, 253, 290, 713, 886, 1025, 1364, 1461, 2030
FalI	AAGNNNNNCTT	4 cut(s)	365, 397, 388, 420
FaqI	GGGAC	2 cut(s)	986, 1688
FatI	CATG	9 cut(s)	87, 249, 286, 709, 882, 1021, 1360, 1457, 2026
FokI	GGATG	6 cut(s)	80, 347, 509, 1097, 1349, 1892
FriOI	GRGCYC	1 cut(s)	249
FspBI	CTAG	8 cut(s)	35, 606, 1055, 1292, 1587, 1835, 2009, 2046
GlaI	GCGC	3 cut(s)	33, 377, 1666
GsaI	CCCAGC	1 cut(s)	1404
GsuI	CTGGAG	4 cut(s)	765, 1155, 1470, 1494
HaeII	RGCGCY	2 cut(s)	35, 379
HaeIII	GGCC	3 cut(s)	269, 686, 723
HapII	CCGG	4 cut(s)	208, 365, 1625, 1709
HgaI	GACGC	2 cut(s)	29, 493
HhaI	GCGC	3 cut(s)	34, 378, 1667
Hin1I	GRCGYC	2 cut(s)	21, 2151
Hin1II	CATG	9 cut(s)	91, 253, 290, 713, 886, 1025, 1364, 1461, 2030
Hin6I	GCGC	3 cut(s)	32, 376, 1665
HinP1I	GCGC	3 cut(s)	32, 376, 1665
HinfI	GANTC	6 cut(s)	576, 1619, 1842, 1848, 1883, 2142
HpaII	CCGG	4 cut(s)	208, 365, 1625, 1709
HphI	GGTGA	4 cut(s)	448, 770, 797, 1139
Hpy166II	GTNNAC	6 cut(s)	391, 526, 528, 748, 761, 1606
Hpy8I	GTNNAC	6 cut(s)	391, 526, 528, 748, 761, 1606
Hpy99I	CGWCG	3 cut(s)	23, 176, 2153
HpyAV	CCTTC	7 cut(s)	34, 107, 433, 1030, 1075, 1268, 1707
HpyCH4III	ACNGT	5 cut(s)	745, 1384, 1795, 1861, 2060
HpyCH4IV	ACGT	4 cut(s)	530, 869, 1477, 1875
HpySE526I	ACGT	4 cut(s)	530, 869, 1477, 1875
Hsp92I	GRCGYC	2 cut(s)	21, 2151
Hsp92II	CATG	9 cut(s)	91, 253, 290, 713, 886, 1025, 1364, 1461, 2030
HspAI	GCGC	3 cut(s)	32, 376, 1665
Kpn2I	TCCGGA	1 cut(s)	364
LmnI	GCTCC	5 cut(s)	784, 1056, 1280, 1372, 1780
LweI	GCATC	7 cut(s)	55, 223, 348, 410, 501, 668, 1942
MaeI	CTAG	8 cut(s)	35, 606, 1055, 1292, 1587, 1835, 2009, 2046
MaeII	ACGT	4 cut(s)	530, 869, 1477, 1875
MaeIII	GTNAC	6 cut(s)	193, 481, 565, 1252, 1871, 2054
MflI	RGATCY	1 cut(s)	1940
MhlI	GDGCHC	2 cut(s)	249, 1066
MlyI	GAGTC	2 cut(s)	1836, 1892
MmeI	TCCRAC	1 cut(s)	78
MroI	TCCGGA	1 cut(s)	364
MroXI	GAANNNNTTC	2 cut(s)	44, 711
MseI	TTAA	7 cut(s)	156, 501, 621, 1121, 1170, 1500, 2118
MslI	CAYNNNNRTG	1 cut(s)	296
MspA1I	CMGCKG	2 cut(s)	1490, 1829
MspI	CCGG	4 cut(s)	208, 365, 1625, 1709
MspR9I	CCNGG	8 cut(s)	164, 208, 560, 783, 1134, 1183, 1550, 1996
Mva1269I	GAATGC	4 cut(s)	545, 602, 1426, 1812
MvaI	CCWGG	7 cut(s)	164, 560, 783, 1134, 1183, 1550, 1996
MvnI	CGCG	2 cut(s)	487, 1665
NciI	CCSGG	1 cut(s)	208
NheI	GCTAGC	2 cut(s)	34, 1291
NlaIII	CATG	9 cut(s)	91, 253, 290, 713, 886, 1025, 1364, 1461, 2030
NlaIV	GGNNCC	5 cut(s)	78, 322, 638, 1005, 1063
NmuCI	GTSAC	3 cut(s)	481, 1252, 1871
NspI	RCATGY	2 cut(s)	713, 2030
NspV	TTCGAA	1 cut(s)	594
PaeR7I	CTCGAG	1 cut(s)	143
PagI	TCATGA	1 cut(s)	1021
PasI	CCCWGGG	1 cut(s)	1182
PceI	AGGCCT	1 cut(s)	269
PciI	ACATGT	1 cut(s)	709
PctI	GAATGC	4 cut(s)	545, 602, 1426, 1812
PdmI	GAANNNNTTC	2 cut(s)	44, 711
PfeI	GAWTC	4 cut(s)	576, 1619, 1848, 2142
PflMI	CCANNNNNTGG	1 cut(s)	830
PfoI	TCCNGGA	2 cut(s)	1132, 1548
Ple19I	CGATCG	1 cut(s)	1933
PleI	GAGTC	2 cut(s)	1836, 1891
PmaCI	CACGTG	1 cut(s)	531
PmlI	CACGTG	1 cut(s)	531
PpsI	GAGTC	2 cut(s)	1836, 1891
Ppu21I	YACGTR	3 cut(s)	531, 870, 1478
PpuMI	RGGWCCY	1 cut(s)	7
PscI	ACATGT	1 cut(s)	709
PshAI	GACNNNNGTC	1 cut(s)	1932
PshBI	ATTAAT	3 cut(s)	1170, 1500, 2118
Psp124BI	GAGCTC	1 cut(s)	249
Psp5II	RGGWCCY	1 cut(s)	7
Psp6I	CCWGG	7 cut(s)	162, 558, 781, 1132, 1181, 1548, 1994
PspCI	CACGTG	1 cut(s)	531
PspFI	CCCAGC	1 cut(s)	1400
PspGI	CCWGG	7 cut(s)	162, 558, 781, 1132, 1181, 1548, 1994
PspN4I	GGNNCC	5 cut(s)	78, 322, 638, 1005, 1063
PspPI	GGNCC	2 cut(s)	7, 320
PspPPI	RGGWCCY	1 cut(s)	7
PstI	CTGCAG	1 cut(s)	1447
PsuI	RGATCY	1 cut(s)	1940
PvuI	CGATCG	1 cut(s)	1933
PvuII	CAGCTG	2 cut(s)	1490, 1829
RsaI	GTAC	4 cut(s)	527, 872, 1351, 1724
RsaNI	GTAC	4 cut(s)	526, 871, 1350, 1723
RseI	CAYNNNNRTG	1 cut(s)	296
SacI	GAGCTC	1 cut(s)	249
SaqAI	TTAA	7 cut(s)	156, 501, 621, 1121, 1170, 1500, 2118
Sau96I	GGNCC	2 cut(s)	7, 320
SchI	GAGTC	2 cut(s)	1836, 1892
ScrFI	CCNGG	8 cut(s)	164, 208, 560, 783, 1134, 1183, 1550, 1996
SduI	GDGCHC	2 cut(s)	249, 1066
SfaNI	GCATC	7 cut(s)	55, 223, 348, 410, 501, 668, 1942
SfcI	CTRYAG	1 cut(s)	1443
Sfr274I	CTCGAG	1 cut(s)	143
SfuI	TTCGAA	1 cut(s)	594
SinI	GGWCC	2 cut(s)	7, 320
SlaI	CTCGAG	1 cut(s)	143
SmiMI	CAYNNNNRTG	1 cut(s)	296
SmlI	CTYRAG	3 cut(s)	143, 1577, 1715
SmoI	CTYRAG	3 cut(s)	143, 1577, 1715
SnaBI	TACGTA	2 cut(s)	870, 1478
SseBI	AGGCCT	1 cut(s)	269
SsiI	CCGC	5 cut(s)	68, 211, 254, 487, 543
SspI	AATATT	1 cut(s)	619
SspMI	CTAG	8 cut(s)	35, 606, 1055, 1292, 1587, 1835, 2009, 2046
SstI	GAGCTC	1 cut(s)	249
StuI	AGGCCT	1 cut(s)	269
StyD4I	CCNGG	8 cut(s)	162, 206, 558, 781, 1132, 1181, 1548, 1994
TaaI	ACNGT	5 cut(s)	745, 1384, 1795, 1861, 2060
TaiI	ACGT	4 cut(s)	533, 872, 1480, 1878
TaqI	TCGA	9 cut(s)	18, 144, 171, 549, 574, 594, 1317, 1555, 2148
TatI	WGTACW	2 cut(s)	525, 1722
TauI	GCSGC	1 cut(s)	256
TfiI	GAWTC	4 cut(s)	576, 1619, 1848, 2142
Tru1I	TTAA	7 cut(s)	156, 501, 621, 1121, 1170, 1500, 2118
Tru9I	TTAA	7 cut(s)	156, 501, 621, 1121, 1170, 1500, 2118
TscAI	CASTG	4 cut(s)	268, 835, 2037, 2065
TseFI	GTSAC	3 cut(s)	481, 1252, 1871
Tsp45I	GTSAC	3 cut(s)	481, 1252, 1871
TspRI	CASTG	4 cut(s)	268, 835, 2037, 2065
Van91I	CCANNNNNTGG	1 cut(s)	830
VpaK11BI	GGWCC	2 cut(s)	7, 320
VspI	ATTAAT	3 cut(s)	1170, 1500, 2118
XapI	RAATTY	4 cut(s)	44, 120, 179, 1011
XbaI	TCTAGA	1 cut(s)	2045
XceI	RCATGY	2 cut(s)	713, 2030
XhoI	CTCGAG	1 cut(s)	143
XmnI	GAANNNNTTC	2 cut(s)	44, 711
XspI	CTAG	8 cut(s)	35, 606, 1055, 1292, 1587, 1835, 2009, 2046

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.