Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005152

Physical Location & Seq

Genomic Coordinates Reverse (-)

42472637 .. 42477126

Gene Structure 4490 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1312600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 648 bp

ATGCCGGAGCCCACGGAGACAGATCATGATCATGACATTCTGATGACTCAGAGGTCTGATGATGGACCAGAGAATATGGATGGTTCGGATCCACATCAGAAGATGCCTCCTGAAGTCAAGCGCTACTTTTATGAACTTATAGGAGATGAGGAAGAACAAGTTGCACGTTTGGCTGAGGATGGTAACATATATGAAAAGCGACCTAGGGGAGTTTGTACAACTGGAAAAGGAAGCAGTGGAGTTGGTCTAACAGTAACTGCTGCTGTTCAGAAGAATCCCGTCACAAATGGATTGGTCCTGGAAGGAGGCGCATTGGTGCTAGCGGATATGGGAATATGTGCTACTGATGAACTTGATAAGACGGATGAATTAGATCGTGCAGCCGTACATGAAGTTATGGAGCAGCAGACTGTCAGCATTGCCAAGGCTGGGATCACTACGTCTTTGAGTGCACGAACTCCTGTCCTTGCTGCAGCTAATCCAGCACGGTACAAATCTTTCTACTTCTTTTTAATCTGGTTCATTTGGGGCATTACTCTTTATAGGGATAGTGATGTTGAGATGGCTAGGCATGTTGTCTATTTTCACCAGAATAAAGTATCACCGGCTCTTGGTTTCAGTCCACTTGAGCCATCAGTTATTCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009743 GO:0009756 GO:0009987 GO:0010033 GO:0010182 GO:0016043 GO:0023052 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051276 GO:0051716 GO:0065007 GO:0070887 GO:0071103 GO:0071310 GO:0071322 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

216

Amino Acids

23.74

Weight (kDa)

4.82

Isoelectric Point (pI)

42.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM	PF00493	65 - 163	5.3e-39	MCM P-loop domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005152)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02060 AT4G02060
fragaria_vesca	FvH4_5g39230 FvH4_5g39230 FvH4_5g39230 FvH4_5g39230
malus_domestica	MD01G1012600.v1.1 MD03G1295400.v1.1 MD11G1312000.v1.1 MD11G1312600.v1.1 MD11G1314500.v1.1
prunus_persica	Prupe.8G272000_v2.0.a1
pyrus_communis	pycom01g04470
rosa_chinensis	RchiOBHm_Chr7g0241501
rosa_laevigata	RLG00000000575
rosa_multiflora	Rmu_co8153082.1_g000001 Rmu_sc0040921.1_g000002
rosa_roxburghii	Rroxscaffold_3G00219090 Rroxscaffold_3G00241100 Rroxscaffold_3G00241110 Rroxscaffold_3G00241120 Rroxscaffold_5G00346280
rosa_rugosa	Rorug03G0267600 Rorug07G0344400 Rorug07G0344500
rosa_samantha	Rh7AG498900 Rh7BG469600 Rh7CG515900 Rh7DG483700
rosa_wichuraiana	Rw7G042140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	52
AciI	CCGC	1 cut(s)	323
AclWI	GGATC	3 cut(s)	83, 96, 440
AcuI	CTGAAG	1 cut(s)	132
AfaI	GTAC	3 cut(s)	217, 387, 491
AfeI	AGCGCT	1 cut(s)	122
AfiI	CCNNNNNNNGG	2 cut(s)	429, 611
AjnI	CCWGG	1 cut(s)	297
AluBI	AGCT	1 cut(s)	476
AluI	AGCT	1 cut(s)	476
Alw21I	GWGCWC	1 cut(s)	454
Alw26I	GTCTC	1 cut(s)	11
Alw44I	GTGCAC	1 cut(s)	450
AlwI	GGATC	3 cut(s)	83, 96, 440
AlwNI	CAGNNNCTG	1 cut(s)	257
Aor51HI	AGCGCT	1 cut(s)	122
ApaLI	GTGCAC	1 cut(s)	450
ApeKI	GCWGC	5 cut(s)	260, 380, 403, 470, 473
AspA2I	CCTAGG	1 cut(s)	203
AspLEI	GCGC	2 cut(s)	123, 311
AspS9I	GGNCC	2 cut(s)	65, 295
AsuHPI	GGTGA	2 cut(s)	578, 594
AsuNHI	GCTAGC	1 cut(s)	319
AvaII	GGWCC	2 cut(s)	65, 295
AvrII	CCTAGG	1 cut(s)	203
BaeGI	GKGCMC	1 cut(s)	454
BamHI	GGATCC	1 cut(s)	88
BanII	GRGCYC	1 cut(s)	12
Bbv12I	GWGCWC	1 cut(s)	454
BbvCI	CCTCAGC	1 cut(s)	174
BbvI	GCAGC	5 cut(s)	247, 392, 415, 457, 485
BccI	CCATC	5 cut(s)	56, 74, 173, 556, 640
BceAI	ACGGC	1 cut(s)	368
BciT130I	CCWGG	1 cut(s)	299
BclI	TGATCA	1 cut(s)	28
BcoDI	GTCTC	1 cut(s)	11
BfaI	CTAG	3 cut(s)	204, 320, 567
BfmI	CTRYAG	1 cut(s)	471
BfoI	RGCGCY	1 cut(s)	124
BisI	GCNGC	5 cut(s)	261, 381, 404, 471, 474
BlnI	CCTAGG	1 cut(s)	203
BlsI	GCNGC	5 cut(s)	262, 382, 405, 472, 475
Bme1390I	CCNGG	1 cut(s)	299
Bme18I	GGWCC	2 cut(s)	65, 295
BmgT120I	GGNCC	2 cut(s)	65, 295
BmiI	GGNNCC	2 cut(s)	9, 90
BmrFI	CCNGG	1 cut(s)	299
BmsI	GCATC	1 cut(s)	93
BmtI	GCTAGC	1 cut(s)	323
Bpu10I	CCTNAGC	1 cut(s)	174
BpuEI	CTTGAG	1 cut(s)	647
BsaBI	GATNNNNATC	2 cut(s)	27, 93
BsaJI	CCNNGG	3 cut(s)	12, 203, 423
Bsc4I	CCNNNNNNNGG	2 cut(s)	429, 611
Bse118I	RCCGGY	1 cut(s)	604
Bse1I	ACTGG	1 cut(s)	226
Bse3DI	GCAATG	1 cut(s)	417
Bse8I	GATNNNNATC	2 cut(s)	27, 93
BseBI	CCWGG	1 cut(s)	299
BseDI	CCNNGG	3 cut(s)	12, 203, 423
BseGI	GGATG	3 cut(s)	85, 184, 370
BseJI	GATNNNNATC	2 cut(s)	27, 93
BseLI	CCNNNNNNNGG	2 cut(s)	429, 611
BseMI	GCAATG	1 cut(s)	417
BseMII	CTCAG	2 cut(s)	62, 165
BseNI	ACTGG	1 cut(s)	226
BseSI	GKGCMC	1 cut(s)	454
BseXI	GCAGC	5 cut(s)	247, 392, 415, 457, 485
BseYI	CCCAGC	1 cut(s)	428
BsgI	GTGCAG	1 cut(s)	399
BsiHKAI	GWGCWC	1 cut(s)	454
BsiSI	CCGG	2 cut(s)	5, 605
BslI	CCNNNNNNNGG	2 cut(s)	429, 611
BsmAI	GTCTC	1 cut(s)	11
Bsp1286I	GDGCHC	2 cut(s)	12, 454
Bsp1407I	TGTACA	1 cut(s)	215
Bsp143I	GATC	5 cut(s)	22, 28, 88, 373, 432
BspACI	CCGC	1 cut(s)	323
BspCNI	CTCAG	2 cut(s)	61, 166
BspHI	TCATGA	2 cut(s)	25, 31
BspLI	GGNNCC	2 cut(s)	9, 90
BspMAI	CTGCAG	1 cut(s)	475
BspOI	GCTAGC	1 cut(s)	323
BspPI	GGATC	3 cut(s)	83, 96, 440
BsrDI	GCAATG	1 cut(s)	417
BsrFI	RCCGGY	1 cut(s)	604
BsrGI	TGTACA	1 cut(s)	215
BsrI	ACTGG	1 cut(s)	226
BssAI	RCCGGY	1 cut(s)	604
BssECI	CCNNGG	3 cut(s)	12, 203, 423
BssMI	GATC	5 cut(s)	22, 28, 88, 373, 432
BssT1I	CCWWGG	2 cut(s)	203, 423
Bst2UI	CCWGG	1 cut(s)	299
Bst4CI	ACNGT	3 cut(s)	253, 412, 489
BstAUI	TGTACA	1 cut(s)	215
BstC8I	GCNNGC	1 cut(s)	321
BstDEI	CTNAG	2 cut(s)	48, 174
BstDSI	CCRYGG	1 cut(s)	12
BstF5I	GGATG	3 cut(s)	85, 184, 370
BstH2I	RGCGCY	1 cut(s)	124
BstHHI	GCGC	2 cut(s)	123, 311
BstKTI	GATC	5 cut(s)	25, 31, 91, 376, 435
BstMAI	GTCTC	1 cut(s)	11
BstMBI	GATC	5 cut(s)	22, 28, 88, 373, 432
BstMWI	GCNNNNNNNGC	2 cut(s)	170, 482
BstNI	CCWGG	1 cut(s)	299
BstNSI	RCATGY	1 cut(s)	575
BstSCI	CCNGG	1 cut(s)	297
BstSFI	CTRYAG	1 cut(s)	471
BstSLI	GKGCMC	1 cut(s)	454
BstV1I	GCAGC	5 cut(s)	247, 392, 415, 457, 485
BstX2I	RGATCY	1 cut(s)	88
BstYI	RGATCY	1 cut(s)	88
BtgI	CCRYGG	1 cut(s)	12
BtsCI	GGATG	3 cut(s)	85, 184, 370
BtsI	GCAGTG	1 cut(s)	241
BtsIMutI	CAGTG	1 cut(s)	241
Cac8I	GCNNGC	1 cut(s)	321
CaiI	CAGNNNCTG	1 cut(s)	257
CciI	TCATGA	2 cut(s)	25, 31
CfoI	GCGC	2 cut(s)	123, 311
Cfr10I	RCCGGY	1 cut(s)	604
Cfr13I	GGNCC	2 cut(s)	65, 295
Csp6I	GTAC	3 cut(s)	216, 386, 490
CviAII	CATG	4 cut(s)	26, 32, 389, 572
CviJI	RGCY	8 cut(s)	10, 173, 383, 428, 476, 566, 608, 631
CviKI_1	RGCY	8 cut(s)	10, 173, 383, 428, 476, 566, 608, 631
CviQI	GTAC	3 cut(s)	216, 386, 490
DdeI	CTNAG	2 cut(s)	48, 174
DpnI	GATC	5 cut(s)	24, 30, 90, 375, 434
DpnII	GATC	5 cut(s)	22, 28, 88, 373, 432
DrdI	GACNNNNNNGTC	1 cut(s)	52
DseDI	GACNNNNNNGTC	1 cut(s)	52
Eco130I	CCWWGG	2 cut(s)	203, 423
Eco24I	GRGCYC	1 cut(s)	12
Eco47I	GGWCC	2 cut(s)	65, 295
Eco47III	AGCGCT	1 cut(s)	122
Eco57I	CTGAAG	1 cut(s)	132
EcoRII	CCWGG	1 cut(s)	297
EcoT14I	CCWWGG	2 cut(s)	203, 423
EcoT38I	GRGCYC	1 cut(s)	12
ErhI	CCWWGG	2 cut(s)	203, 423
FaeI	CATG	4 cut(s)	29, 35, 392, 575
FalI	AAGNNNNNCTT	2 cut(s)	110, 142
FatI	CATG	4 cut(s)	25, 31, 388, 571
FbaI	TGATCA	1 cut(s)	28
Fnu4HI	GCNGC	5 cut(s)	261, 381, 404, 471, 474
FokI	GGATG	3 cut(s)	92, 191, 377
FriOI	GRGCYC	1 cut(s)	12
Fsp4HI	GCNGC	5 cut(s)	261, 381, 404, 471, 474
FspBI	CTAG	3 cut(s)	204, 320, 567
GlaI	GCGC	2 cut(s)	122, 310
GluI	GCNGC	5 cut(s)	261, 381, 404, 471, 474
GsaI	CCCAGC	1 cut(s)	432
HaeII	RGCGCY	1 cut(s)	124
HapII	CCGG	2 cut(s)	5, 605
HhaI	GCGC	2 cut(s)	123, 311
Hin1II	CATG	4 cut(s)	29, 35, 392, 575
Hin6I	GCGC	2 cut(s)	121, 309
HinP1I	GCGC	2 cut(s)	121, 309
HinfI	GANTC	2 cut(s)	46, 274
HpaII	CCGG	2 cut(s)	5, 605
HphI	GGTGA	2 cut(s)	578, 594
Hpy166II	GTNNAC	2 cut(s)	452, 623
Hpy188I	TCNGA	6 cut(s)	42, 51, 58, 88, 99, 270
Hpy188III	TCNNGA	3 cut(s)	26, 32, 110
Hpy8I	GTNNAC	2 cut(s)	452, 623
HpyAV	CCTTC	1 cut(s)	296
HpyCH4III	ACNGT	3 cut(s)	253, 412, 489
HpyCH4IV	ACGT	2 cut(s)	166, 440
HpyCH4V	TGCA	4 cut(s)	164, 380, 452, 473
HpyF10VI	GCNNNNNNNGC	2 cut(s)	170, 482
HpyF3I	CTNAG	2 cut(s)	48, 174
HpySE526I	ACGT	2 cut(s)	166, 440
Hsp92II	CATG	4 cut(s)	29, 35, 392, 575
HspAI	GCGC	2 cut(s)	121, 309
Ksp22I	TGATCA	1 cut(s)	28
Kzo9I	GATC	5 cut(s)	22, 28, 88, 373, 432
LmnI	GCTCC	2 cut(s)	7, 400
Lsp1109I	GCAGC	5 cut(s)	247, 392, 415, 457, 485
LweI	GCATC	1 cut(s)	93
MaeI	CTAG	3 cut(s)	204, 320, 567
MaeII	ACGT	2 cut(s)	166, 440
MaeIII	GTNAC	3 cut(s)	182, 253, 280
MalI	GATC	5 cut(s)	24, 30, 90, 375, 434
MboI	GATC	5 cut(s)	22, 28, 88, 373, 432
MboII	GAAGA	3 cut(s)	112, 164, 283
MflI	RGATCY	1 cut(s)	88
MhlI	GDGCHC	2 cut(s)	12, 454
MluCI	AATT	1 cut(s)	368
MlyI	GAGTC	1 cut(s)	40
MnlI	CCTC	5 cut(s)	45, 117, 142, 169, 299
MseI	TTAA	1 cut(s)	512
MslI	CAYNNNNRTG	2 cut(s)	30, 41
MspI	CCGG	2 cut(s)	5, 605
MspR9I	CCNGG	1 cut(s)	299
MvaI	CCWGG	1 cut(s)	299
MwoI	GCNNNNNNNGC	2 cut(s)	170, 482
NdeII	GATC	5 cut(s)	22, 28, 88, 373, 432
NheI	GCTAGC	1 cut(s)	319
NlaIII	CATG	4 cut(s)	29, 35, 392, 575
NlaIV	GGNNCC	2 cut(s)	9, 90
NmuCI	GTSAC	1 cut(s)	280
NspI	RCATGY	1 cut(s)	575
PagI	TCATGA	2 cut(s)	25, 31
PfeI	GAWTC	1 cut(s)	274
PfoI	TCCNGGA	1 cut(s)	297
PkrI	GCNGC	5 cut(s)	262, 382, 405, 472, 475
PleI	GAGTC	1 cut(s)	40
PpsI	GAGTC	1 cut(s)	40
Psp6I	CCWGG	1 cut(s)	297
PspFI	CCCAGC	1 cut(s)	428
PspGI	CCWGG	1 cut(s)	297
PspN4I	GGNNCC	2 cut(s)	9, 90
PspPI	GGNCC	2 cut(s)	65, 295
PstI	CTGCAG	1 cut(s)	475
PstNI	CAGNNNCTG	1 cut(s)	257
PsuI	RGATCY	1 cut(s)	88
RsaI	GTAC	3 cut(s)	217, 387, 491
RsaNI	GTAC	3 cut(s)	216, 386, 490
RseI	CAYNNNNRTG	2 cut(s)	30, 41
SaqAI	TTAA	1 cut(s)	512
SatI	GCNGC	5 cut(s)	261, 381, 404, 471, 474
Sau3AI	GATC	5 cut(s)	22, 28, 88, 373, 432
Sau96I	GGNCC	2 cut(s)	65, 295
SchI	GAGTC	1 cut(s)	40
ScrFI	CCNGG	1 cut(s)	299
SduI	GDGCHC	2 cut(s)	12, 454
SetI	ASST	5 cut(s)	56, 169, 205, 443, 478
SfaNI	GCATC	1 cut(s)	93
SfcI	CTRYAG	1 cut(s)	471
SinI	GGWCC	2 cut(s)	65, 295
SmiMI	CAYNNNNRTG	2 cut(s)	30, 41
SmlI	CTYRAG	1 cut(s)	626
SmoI	CTYRAG	1 cut(s)	626
Sse9I	AATT	1 cut(s)	368
SsiI	CCGC	1 cut(s)	323
SspMI	CTAG	3 cut(s)	204, 320, 567
StyD4I	CCNGG	1 cut(s)	297
StyI	CCWWGG	2 cut(s)	203, 423
TaaI	ACNGT	3 cut(s)	253, 412, 489
TaiI	ACGT	2 cut(s)	169, 443
TasI	AATT	1 cut(s)	368
TatI	WGTACW	1 cut(s)	215
TfiI	GAWTC	1 cut(s)	274
Tru1I	TTAA	1 cut(s)	512
Tru9I	TTAA	1 cut(s)	512
TscAI	CASTG	1 cut(s)	241
TseFI	GTSAC	1 cut(s)	280
TseI	GCWGC	5 cut(s)	260, 380, 403, 470, 473
Tsp45I	GTSAC	1 cut(s)	280
TspDTI	ATGAA	6 cut(s)	147, 207, 363, 381, 405, 511
TspGWI	ACGGA	2 cut(s)	29, 377
TspRI	CASTG	1 cut(s)	241
VneI	GTGCAC	1 cut(s)	450
VpaK11BI	GGWCC	2 cut(s)	65, 295
XceI	RCATGY	1 cut(s)	575
XmaJI	CCTAGG	1 cut(s)	203
XspI	CTAG	3 cut(s)	204, 320, 567

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.