MD03G1298600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007809
Physical Location & Seq
Forward (+)
37416191 .. 37416640
450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1298600.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGAAGGTGTCTCTACAAGCGTTTGCAAGGGTTTGAGAGGCTACTGGAGGAGGAGAGGCTACGAGAAGCTAAGTGGGTCGGGTCGACAGCACAAGAACCGGACGGAGCTGGGTTCGATCCGTAAAAGGCGCGTATGGCGGATCCGGATCAAAATCGGTCCGAAGCTGAGCTTCCTCAGAAGAATCCCTTCGCCCAAGAATTTGTTGGTGTGGCTACGGGACGCCTATGTCAAGCTGATGCTGGGATTCGCTAACTCGCAGGTCTTTAGTACCGGCTACGGTGGGTCCGTCTGCGACTACGGGGTCGGCTCCTTTGGAAAGGGTCCAGTTAAGGAGTATGATGAGAAAATGATCATCGAGATCTACAAGTCCCTGATGCTGGCGCAAGGTCAATTGGTGCCCCGCGAAGCACCCAAGCTCGGCTCAGAAATCGTTCACCACCGGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.98

Weight (kDa)

10.44

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 227, 302
Acc36I ACCTGC 1 cut(s) 250
AccB1I GGYRCC 1 cut(s) 397
AccI GTMKAC 1 cut(s) 85
AccII CGCG 2 cut(s) 132, 405
AccIII TCCGGA 1 cut(s) 144
AciI CCGC 2 cut(s) 139, 403
AclWI GGATC 4 cut(s) 112, 136, 149, 155
AcsI RAATTY 1 cut(s) 199
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 1 cut(s) 271
AfiI CCNNNNNNNGG 2 cut(s) 379, 419
AluBI AGCT 6 cut(s) 70, 109, 166, 171, 235, 418
AluI AGCT 6 cut(s) 70, 109, 166, 171, 235, 418
Alw26I GTCTC 1 cut(s) 16
AlwI GGATC 4 cut(s) 112, 136, 149, 155
Aor13HI TCCGGA 1 cut(s) 144
ApoI RAATTY 1 cut(s) 199
Asp700I GAANNNNTTC 2 cut(s) 187, 432
AspLEI GCGC 2 cut(s) 132, 385
AspS9I GGNCC 3 cut(s) 158, 285, 323
AsuHPI GGTGA 1 cut(s) 428
AvaII GGWCC 3 cut(s) 158, 285, 323
BaeGI GKGCMC 1 cut(s) 402
BamHI GGATCC 1 cut(s) 141
BanI GGYRCC 1 cut(s) 397
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 1 cut(s) 16
BfuAI ACCTGC 1 cut(s) 250
BglII AGATCT 1 cut(s) 360
BlpI GCTNAGC 1 cut(s) 167
Bme18I GGWCC 3 cut(s) 158, 285, 323
BmgT120I GGNCC 3 cut(s) 158, 285, 323
BmiI GGNNCC 5 cut(s) 143, 286, 310, 324, 399
BmsI GCATC 2 cut(s) 228, 366
BpmI CTGGAG 1 cut(s) 67
Bpu1102I GCTNAGC 1 cut(s) 167
BsaBI GATNNNNATC 2 cut(s) 146, 152
BsaHI GRCGYC 1 cut(s) 222
BsaWI WCCGGW 2 cut(s) 99, 144
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 419
Bse118I RCCGGY 2 cut(s) 272, 441
Bse1I ACTGG 2 cut(s) 50, 326
Bse8I GATNNNNATC 2 cut(s) 146, 152
BseAI TCCGGA 1 cut(s) 144
BseJI GATNNNNATC 2 cut(s) 146, 152
BseLI CCNNNNNNNGG 2 cut(s) 379, 419
BseMII CTCAG 3 cut(s) 158, 190, 438
BseNI ACTGG 2 cut(s) 50, 326
BseRI GAGGAG 2 cut(s) 64, 67
BseSI GKGCMC 1 cut(s) 402
BseYI CCCAGC 2 cut(s) 109, 241
Bsh1236I CGCG 2 cut(s) 132, 405
BshNI GGYRCC 1 cut(s) 397
BsiSI CCGG 4 cut(s) 100, 145, 273, 442
BslFI GGGAC 2 cut(s) 233, 355
BslI CCNNNNNNNGG 2 cut(s) 379, 419
BsmAI GTCTC 1 cut(s) 16
BsmFI GGGAC 2 cut(s) 233, 355
Bsp1286I GDGCHC 1 cut(s) 402
Bsp13I TCCGGA 1 cut(s) 144
Bsp143I GATC 5 cut(s) 117, 141, 147, 351, 360
Bsp1720I GCTNAGC 1 cut(s) 167
BspACI CCGC 2 cut(s) 139, 403
BspCNI CTCAG 3 cut(s) 159, 189, 437
BspEI TCCGGA 1 cut(s) 144
BspFNI CGCG 2 cut(s) 132, 405
BspLI GGNNCC 5 cut(s) 143, 286, 310, 324, 399
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 4 cut(s) 112, 136, 149, 155
BspT107I GGYRCC 1 cut(s) 397
BsrFI RCCGGY 2 cut(s) 272, 441
BsrI ACTGG 2 cut(s) 50, 326
BssAI RCCGGY 2 cut(s) 272, 441
BssMI GATC 5 cut(s) 117, 141, 147, 351, 360
BssNI GRCGYC 1 cut(s) 222
Bst4CI ACNGT 1 cut(s) 281
BstACI GRCGYC 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 4 cut(s) 71, 167, 176, 424
BstFNI CGCG 2 cut(s) 132, 405
BstHHI GCGC 2 cut(s) 132, 385
BstKTI GATC 5 cut(s) 120, 144, 150, 354, 363
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 5 cut(s) 117, 141, 147, 351, 360
BstMWI GCNNNNNNNGC 1 cut(s) 136
BstSLI GKGCMC 1 cut(s) 402
BstUI CGCG 2 cut(s) 132, 405
BstX2I RGATCY 2 cut(s) 141, 360
BstYI RGATCY 2 cut(s) 141, 360
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 1 cut(s) 381
CfoI GCGC 2 cut(s) 132, 385
Cfr10I RCCGGY 2 cut(s) 272, 441
Cfr13I GGNCC 3 cut(s) 158, 285, 323
CpoI CGGWCCG 1 cut(s) 158
CseI GACGC 1 cut(s) 230
Csp6I GTAC 1 cut(s) 270
CspI CGGWCCG 1 cut(s) 158
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 4 cut(s) 71, 167, 176, 424
DpnI GATC 5 cut(s) 119, 143, 149, 353, 362
DpnII GATC 5 cut(s) 117, 141, 147, 351, 360
DrdI GACNNNNNNGTC 2 cut(s) 227, 302
DseDI GACNNNNNNGTC 2 cut(s) 227, 302
EciI GGCGGA 1 cut(s) 154
Eco47I GGWCC 3 cut(s) 158, 285, 323
FaiI YATR 3 cut(s) 136, 228, 339
FalI AAGNNNNNCTT 4 cut(s) 155, 187, 172, 204
FaqI GGGAC 2 cut(s) 233, 355
FauI CCCGC 1 cut(s) 410
FbaI TGATCA 1 cut(s) 351
FblI GTMKAC 1 cut(s) 85
GlaI GCGC 2 cut(s) 131, 384
GsaI CCCAGC 2 cut(s) 113, 245
GsuI CTGGAG 1 cut(s) 67
HapII CCGG 4 cut(s) 100, 145, 273, 442
HgaI GACGC 1 cut(s) 230
HhaI GCGC 2 cut(s) 132, 385
Hin1I GRCGYC 1 cut(s) 222
Hin6I GCGC 2 cut(s) 130, 383
HinP1I GCGC 2 cut(s) 130, 383
HincII GTYRAC 1 cut(s) 86
HindII GTYRAC 1 cut(s) 86
HinfI GANTC 2 cut(s) 183, 246
HpaII CCGG 4 cut(s) 100, 145, 273, 442
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 2 cut(s) 86, 436
Hpy188I TCNGA 3 cut(s) 162, 179, 427
Hpy188III TCNNGA 2 cut(s) 145, 358
Hpy8I GTNNAC 2 cut(s) 86, 436
HpyAV CCTTC 1 cut(s) 198
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4V TGCA 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 1 cut(s) 136
HpyF3I CTNAG 4 cut(s) 71, 167, 176, 424
Hsp92I GRCGYC 1 cut(s) 222
HspAI GCGC 2 cut(s) 130, 383
Kpn2I TCCGGA 1 cut(s) 144
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 5 cut(s) 117, 141, 147, 351, 360
LmnI GCTCC 2 cut(s) 106, 314
LweI GCATC 2 cut(s) 228, 366
MalI GATC 5 cut(s) 119, 143, 149, 353, 362
MboI GATC 5 cut(s) 117, 141, 147, 351, 360
MboII GAAGA 1 cut(s) 192
MfeI CAATTG 1 cut(s) 392
MflI RGATCY 2 cut(s) 141, 360
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 2 cut(s) 199, 392
MnlI CCTC 5 cut(s) 32, 42, 45, 50, 185
MroI TCCGGA 1 cut(s) 144
MroXI GAANNNNTTC 2 cut(s) 187, 432
MseI TTAA 1 cut(s) 330
MspI CCGG 4 cut(s) 100, 145, 273, 442
MunI CAATTG 1 cut(s) 392
MvnI CGCG 2 cut(s) 132, 405
MwoI GCNNNNNNNGC 1 cut(s) 136
NdeII GATC 5 cut(s) 117, 141, 147, 351, 360
NlaIV GGNNCC 5 cut(s) 143, 286, 310, 324, 399
NmeAIII GCCGAG 1 cut(s) 399
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PdmI GAANNNNTTC 2 cut(s) 187, 432
PfeI GAWTC 2 cut(s) 183, 246
PspFI CCCAGC 2 cut(s) 109, 241
PspN4I GGNNCC 5 cut(s) 143, 286, 310, 324, 399
PspPI GGNCC 3 cut(s) 158, 285, 323
PsuI RGATCY 2 cut(s) 141, 360
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
Rsr2I CGGWCCG 1 cut(s) 158
RsrII CGGWCCG 1 cut(s) 158
SalI GTCGAC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 330
Sau3AI GATC 5 cut(s) 117, 141, 147, 351, 360
Sau96I GGNCC 3 cut(s) 158, 285, 323
SduI GDGCHC 1 cut(s) 402
SetI ASST 9 cut(s) 10, 72, 111, 168, 173, 237, 264, 391, 420
SfaNI GCATC 2 cut(s) 228, 366
SinI GGWCC 3 cut(s) 158, 285, 323
Sse9I AATT 2 cut(s) 199, 392
SsiI CCGC 2 cut(s) 139, 403
TaaI ACNGT 1 cut(s) 281
TaqI TCGA 3 cut(s) 85, 116, 357
TaqII GACCGA 1 cut(s) 146
TasI AATT 2 cut(s) 199, 392
TfiI GAWTC 2 cut(s) 183, 246
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TspGWI ACGGA 3 cut(s) 110, 119, 277
VpaK11BI GGWCC 3 cut(s) 158, 285, 323
XapI RAATTY 1 cut(s) 199
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 85
XmnI GAANNNNTTC 2 cut(s) 187, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.