pycom03g23660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007809
Physical Location & Seq
Forward (+)
23475510 .. 23475962
453 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23660.1

Sequence Viewer

Length: 453 bp
ATGGAAGGTGTCTCTACAAGCGTTTGCAAGGTTTTGAGAGGCTACTGGAGGAGGGGGAGAGGCTACGAGAAGCTAAGTGGGTCGGGTCGACGGCACAAGAACCGGACGGAGCTGGGTTCGATCCGTAAAAGGCGCGTATGTCGGATCCGGATCAAAATCGCTCCGAAGCTGAGCTTCCTCAGAAGAATCCCTTCGCCCAAGAATTTGTTGGTGTGGCTACGGGACGCCTATGTCAAGCTGATGCTGGGATTCGCTAACTCGCAGGTCTTTAGTACCGGCTACGGTGGGTCCGTCTGCGACTACGGGGTCGGCTCCTTTGGAAAGCGTCCAGTTAAGGAGTATGATGAGAAAATGATCATCGAGATCTACAAGTCCCTGATGCTGGCGCAAGGTCAATTGGTGCCCCGCGAAGCACCCAAGCTCGGCTCAGAAATCATTCACCACCGGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

17.17

Weight (kDa)

10.54

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 230, 305
Acc36I ACCTGC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 400
AccI GTMKAC 1 cut(s) 88
AccII CGCG 2 cut(s) 135, 408
AccIII TCCGGA 1 cut(s) 147
AciI CCGC 1 cut(s) 406
AclWI GGATC 4 cut(s) 115, 139, 152, 158
AcsI RAATTY 1 cut(s) 202
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 1 cut(s) 274
AfiI CCNNNNNNNGG 2 cut(s) 382, 422
AluBI AGCT 6 cut(s) 73, 112, 169, 174, 238, 421
AluI AGCT 6 cut(s) 73, 112, 169, 174, 238, 421
Alw26I GTCTC 1 cut(s) 16
AlwI GGATC 4 cut(s) 115, 139, 152, 158
Aor13HI TCCGGA 1 cut(s) 147
ApoI RAATTY 1 cut(s) 202
Asp700I GAANNNNTTC 2 cut(s) 190, 435
AspLEI GCGC 2 cut(s) 135, 388
AspS9I GGNCC 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 431
AvaII GGWCC 1 cut(s) 288
BaeGI GKGCMC 1 cut(s) 405
BamHI GGATCC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 400
BceAI ACGGC 1 cut(s) 107
BclI TGATCA 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 16
BfuAI ACCTGC 1 cut(s) 253
BglII AGATCT 1 cut(s) 363
BlpI GCTNAGC 1 cut(s) 170
Bme18I GGWCC 1 cut(s) 288
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 4 cut(s) 146, 289, 313, 402
BmsI GCATC 2 cut(s) 231, 369
BpmI CTGGAG 1 cut(s) 67
Bpu1102I GCTNAGC 1 cut(s) 170
BsaBI GATNNNNATC 2 cut(s) 149, 155
BsaHI GRCGYC 1 cut(s) 225
BsaWI WCCGGW 2 cut(s) 102, 147
Bsc4I CCNNNNNNNGG 2 cut(s) 382, 422
Bse118I RCCGGY 2 cut(s) 275, 444
Bse1I ACTGG 2 cut(s) 50, 329
Bse8I GATNNNNATC 2 cut(s) 149, 155
BseAI TCCGGA 1 cut(s) 147
BseJI GATNNNNATC 2 cut(s) 149, 155
BseLI CCNNNNNNNGG 2 cut(s) 382, 422
BseMII CTCAG 3 cut(s) 161, 193, 441
BseNI ACTGG 2 cut(s) 50, 329
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 405
BseYI CCCAGC 2 cut(s) 112, 244
Bsh1236I CGCG 2 cut(s) 135, 408
BshNI GGYRCC 1 cut(s) 400
BsiSI CCGG 4 cut(s) 103, 148, 276, 445
BslFI GGGAC 2 cut(s) 236, 358
BslI CCNNNNNNNGG 2 cut(s) 382, 422
BsmAI GTCTC 1 cut(s) 16
BsmFI GGGAC 2 cut(s) 236, 358
Bsp1286I GDGCHC 1 cut(s) 405
Bsp13I TCCGGA 1 cut(s) 147
Bsp143I GATC 5 cut(s) 120, 144, 150, 354, 363
Bsp1720I GCTNAGC 1 cut(s) 170
BspACI CCGC 1 cut(s) 406
BspCNI CTCAG 3 cut(s) 162, 192, 440
BspEI TCCGGA 1 cut(s) 147
BspFNI CGCG 2 cut(s) 135, 408
BspLI GGNNCC 4 cut(s) 146, 289, 313, 402
BspMI ACCTGC 1 cut(s) 253
BspPI GGATC 4 cut(s) 115, 139, 152, 158
BspT107I GGYRCC 1 cut(s) 400
BsrFI RCCGGY 2 cut(s) 275, 444
BsrI ACTGG 2 cut(s) 50, 329
BssAI RCCGGY 2 cut(s) 275, 444
BssMI GATC 5 cut(s) 120, 144, 150, 354, 363
BssNI GRCGYC 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 284
BstACI GRCGYC 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 4 cut(s) 74, 170, 179, 427
BstFNI CGCG 2 cut(s) 135, 408
BstHHI GCGC 2 cut(s) 135, 388
BstKTI GATC 5 cut(s) 123, 147, 153, 357, 366
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 5 cut(s) 120, 144, 150, 354, 363
BstSLI GKGCMC 1 cut(s) 405
BstUI CGCG 2 cut(s) 135, 408
BstX2I RGATCY 2 cut(s) 144, 363
BstYI RGATCY 2 cut(s) 144, 363
BveI ACCTGC 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 384
CfoI GCGC 2 cut(s) 135, 388
Cfr10I RCCGGY 2 cut(s) 275, 444
Cfr13I GGNCC 1 cut(s) 288
CseI GACGC 2 cut(s) 233, 314
Csp6I GTAC 1 cut(s) 273
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 4 cut(s) 74, 170, 179, 427
DpnI GATC 5 cut(s) 122, 146, 152, 356, 365
DpnII GATC 5 cut(s) 120, 144, 150, 354, 363
DrdI GACNNNNNNGTC 2 cut(s) 230, 305
DseDI GACNNNNNNGTC 2 cut(s) 230, 305
Eco47I GGWCC 1 cut(s) 288
FaiI YATR 3 cut(s) 139, 231, 342
FalI AAGNNNNNCTT 4 cut(s) 158, 190, 175, 207
FaqI GGGAC 2 cut(s) 236, 358
FauI CCCGC 1 cut(s) 413
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 1 cut(s) 88
GlaI GCGC 2 cut(s) 134, 387
GsaI CCCAGC 2 cut(s) 116, 248
GsuI CTGGAG 1 cut(s) 67
HapII CCGG 4 cut(s) 103, 148, 276, 445
HgaI GACGC 2 cut(s) 233, 314
HhaI GCGC 2 cut(s) 135, 388
Hin1I GRCGYC 1 cut(s) 225
Hin6I GCGC 2 cut(s) 133, 386
HinP1I GCGC 2 cut(s) 133, 386
HincII GTYRAC 1 cut(s) 89
HindII GTYRAC 1 cut(s) 89
HinfI GANTC 2 cut(s) 186, 249
HpaII CCGG 4 cut(s) 103, 148, 276, 445
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 89
Hpy188I TCNGA 4 cut(s) 144, 165, 182, 430
Hpy188III TCNNGA 2 cut(s) 148, 361
Hpy8I GTNNAC 1 cut(s) 89
Hpy99I CGWCG 1 cut(s) 93
HpyAV CCTTC 1 cut(s) 201
HpyCH4III ACNGT 1 cut(s) 284
HpyCH4V TGCA 1 cut(s) 27
HpyF3I CTNAG 4 cut(s) 74, 170, 179, 427
Hsp92I GRCGYC 1 cut(s) 225
HspAI GCGC 2 cut(s) 133, 386
Kpn2I TCCGGA 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 5 cut(s) 120, 144, 150, 354, 363
LmnI GCTCC 3 cut(s) 109, 166, 317
LweI GCATC 2 cut(s) 231, 369
MalI GATC 5 cut(s) 122, 146, 152, 356, 365
MboI GATC 5 cut(s) 120, 144, 150, 354, 363
MboII GAAGA 1 cut(s) 195
MfeI CAATTG 1 cut(s) 395
MflI RGATCY 2 cut(s) 144, 363
MhlI GDGCHC 1 cut(s) 405
MluCI AATT 2 cut(s) 202, 395
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 5 cut(s) 32, 42, 45, 53, 188
MroI TCCGGA 1 cut(s) 147
MroXI GAANNNNTTC 2 cut(s) 190, 435
MseI TTAA 1 cut(s) 333
MspI CCGG 4 cut(s) 103, 148, 276, 445
MunI CAATTG 1 cut(s) 395
MvnI CGCG 2 cut(s) 135, 408
NdeII GATC 5 cut(s) 120, 144, 150, 354, 363
NlaIV GGNNCC 4 cut(s) 146, 289, 313, 402
NmeAIII GCCGAG 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PdmI GAANNNNTTC 2 cut(s) 190, 435
PfeI GAWTC 2 cut(s) 186, 249
PspFI CCCAGC 2 cut(s) 112, 244
PspN4I GGNNCC 4 cut(s) 146, 289, 313, 402
PspPI GGNCC 1 cut(s) 288
PsuI RGATCY 2 cut(s) 144, 363
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
SalI GTCGAC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 333
Sau3AI GATC 5 cut(s) 120, 144, 150, 354, 363
Sau96I GGNCC 1 cut(s) 288
SduI GDGCHC 1 cut(s) 405
SfaNI GCATC 2 cut(s) 231, 369
SinI GGWCC 1 cut(s) 288
Sse9I AATT 2 cut(s) 202, 395
SsiI CCGC 1 cut(s) 406
TaaI ACNGT 1 cut(s) 284
TaqI TCGA 3 cut(s) 88, 119, 360
TasI AATT 2 cut(s) 202, 395
TfiI GAWTC 2 cut(s) 186, 249
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TspGWI ACGGA 3 cut(s) 113, 122, 280
VpaK11BI GGWCC 1 cut(s) 288
XapI RAATTY 1 cut(s) 202
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XmiI GTMKAC 1 cut(s) 88
XmnI GAANNNNTTC 2 cut(s) 190, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.