MD04G1000400.v1.1

Importin-beta N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003769
Physical Location & Seq
Forward (+)
37796 .. 43481
5686 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1000400.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGATTTCTGTTCTTATTGGACATATGAGTGAAGTTATGACATTTGCAAACAAGCTGGTTCTATTTTTCCAGAAGGCTTGGGAGGAATCTTCCAGTGAATGCCTTCTGCAGATTCAACTTCTTGTTGCTTTGAGGAACTTTGTAGTTGCACTTGGCTATCAGTCACCCATTTGCTACGATATATTACTGCCCATTCTGCAAAAGGGAATTGATATAAATAGCCCTGATGAGCTCAATCTTCTTGAGGACAGCATGCTGTTATGGGAGGCCACACTTTCTCATGCTCCTACTATGGTGCCTCAACTACTTGCATATTTTCCCTATCTTGTAAAAATCATGGAAAGAAGTTTCGATCACCTGCAGGTTGCAGTCGATATAACTGAAGACTACATTATCTTAGGTGGATCCGAATTTCTTAGTATGCATGCTTCAAGTGTTGCTCAAATTCTAGATCTGGTTGTTGGAAATGTGAATGATAGAGGACTACTTTCAGTTCTTCCTGTCATTGATATTCTAGTACAGTGTTTCCCTATAGAAGTGCCCCAGTTGATCAGCAGTACATTACAAAAATTAATTGTTGTATGTTTGAGTGGAGGCGACGACCAAGATCCTCCTAAGACAGCTGTTAAAGCATCTGCAGCTGCCATCCTGGCAAGAGTTTTGGTTATGAGTACCAATTACCTAGCCCGTTTAACATCAGAACCATCACTTTTGTCGTTCCTTCCGAAGTCAGGTGTCCCAACTGAGGAAAATGTACTTCTTTGTCTTGTTGACGTATGGCTTGACAAGGTAGACAACGTATCTTCCATCCAAAGGAAGACATATGGCCTAGCCCTTTCAATAATTCTGACATTAAGACTTCCTCAAGTCCTCAACAAACTTGATCAGATCCTAAGTGTGTGTACGACTGTAATTTTGGGTGGGAACGATGACTTAACAGAGGAATCGAGTGGTGATAACATAAGCTCGAGCGGGTCCCTTAGCAAGGGTACCATTCTGAGTAAAGAATTCAGAAGAAGACAGCTCAAGGTTTCAGACCCTATCAACCAAATGTCTCTAGAGGCTTCAGTGAGGGAAAATCTCCAAACATGTGCTACCCTTCATGGAGAATCTTTTAATAAGGCGATTGGTTGCATGCATCCCTCAGCATTTTCACAATTGAAGCAGGCATTAAAGATGGAGTAG

Protein Analysis

395

Amino Acids

43.23

Weight (kDa)

4.9

Isoelectric Point (pI)

61.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_IPO11 PF25758 39 - 391 3.1e-71 Importin-11-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 366
Acc36I ACCTGC 2 cut(s) 352, 366
Acc65I GGTACC 1 cut(s) 989
AccB1I GGYRCC 2 cut(s) 295, 989
AccBSI CCGCTC 1 cut(s) 972
AccI GTMKAC 1 cut(s) 792
AciI CCGC 1 cut(s) 972
AclWI GGATC 4 cut(s) 399, 412, 602, 883
AcsI RAATTY 3 cut(s) 410, 444, 1007
AcuI CTGAAG 2 cut(s) 402, 1050
AfaI GTAC 6 cut(s) 519, 559, 673, 756, 904, 991
AfiI CCNNNNNNNGG 4 cut(s) 731, 745, 813, 985
AflIII ACRYGT 1 cut(s) 1088
AgsI TTSAA 4 cut(s) 116, 432, 840, 1162
AjnI CCWGG 1 cut(s) 648
AluBI AGCT 6 cut(s) 55, 232, 623, 641, 966, 1024
AluI AGCT 6 cut(s) 55, 232, 623, 641, 966, 1024
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 1 cut(s) 1059
AlwI GGATC 4 cut(s) 399, 412, 602, 883
Ama87I CYCGRG 1 cut(s) 967
AoxI GGCC 2 cut(s) 267, 826
ApeKI GCWGC 2 cut(s) 638, 641
ApoI RAATTY 3 cut(s) 410, 444, 1007
ArsI GACNNNNNNTTYG 2 cut(s) 898, 930
AseI ATTAAT 1 cut(s) 572
Asp700I GAANNNNTTC 1 cut(s) 102
Asp718I GGTACC 1 cut(s) 989
AspS9I GGNCC 1 cut(s) 975
AsuHPI GGTGA 3 cut(s) 156, 347, 965
AvaI CYCGRG 1 cut(s) 967
AvaII GGWCC 1 cut(s) 975
BaeGI GKGCMC 1 cut(s) 543
BamHI GGATCC 1 cut(s) 404
BanI GGYRCC 2 cut(s) 295, 989
BanII GRGCYC 1 cut(s) 234
BarI GAAGNNNNNNTAC 2 cut(s) 412, 444
BbsI GAAGAC 3 cut(s) 390, 824, 1024
Bbv12I GWGCWC 1 cut(s) 234
BbvCI CCTCAGC 1 cut(s) 1144
BbvI GCAGC 2 cut(s) 628, 650
BccI CCATC 4 cut(s) 653, 712, 815, 1171
BciT130I CCWGG 1 cut(s) 650
BclI TGATCA 2 cut(s) 549, 883
BcoDI GTCTC 1 cut(s) 1059
BfaI CTAG 5 cut(s) 449, 515, 683, 830, 1058
BfmI CTRYAG 4 cut(s) 107, 359, 531, 636
BfuAI ACCTGC 2 cut(s) 352, 366
BglI GCCNNNNNGGC 1 cut(s) 650
BglII AGATCT 1 cut(s) 451
BisI GCNGC 2 cut(s) 639, 642
BlsI GCNGC 2 cut(s) 640, 643
Bme1390I CCNGG 1 cut(s) 650
Bme18I GGWCC 1 cut(s) 975
BmeT110I CYCGRG 1 cut(s) 967
BmgT120I GGNCC 1 cut(s) 975
BmiI GGNNCC 5 cut(s) 297, 406, 976, 977, 991
BmrFI CCNGG 1 cut(s) 650
BmrI ACTGGG 1 cut(s) 538
BmsI GCATC 2 cut(s) 641, 1147
BmuI ACTGGG 1 cut(s) 538
BpiI GAAGAC 3 cut(s) 390, 824, 1024
Bpu10I CCTNAGC 2 cut(s) 980, 1144
BpuEI CTTGAG 3 cut(s) 263, 849, 1010
Bsc4I CCNNNNNNNGG 4 cut(s) 731, 745, 813, 985
Bse1I ACTGG 2 cut(s) 93, 544
BseBI CCWGG 1 cut(s) 650
BseGI GGATG 3 cut(s) 645, 807, 1138
BseLI CCNNNNNNNGG 4 cut(s) 731, 745, 813, 985
BseMII CTCAG 3 cut(s) 735, 989, 1158
BseNI ACTGG 2 cut(s) 93, 544
BseSI GKGCMC 1 cut(s) 543
BseXI GCAGC 2 cut(s) 628, 650
BshFI GGCC 2 cut(s) 269, 828
BshNI GGYRCC 2 cut(s) 295, 989
BsiHKAI GWGCWC 1 cut(s) 234
BsiHKCI CYCGRG 1 cut(s) 967
BslFI GGGAC 2 cut(s) 722, 961
BslI CCNNNNNNNGG 4 cut(s) 731, 745, 813, 985
BsmAI GTCTC 1 cut(s) 1059
BsmFI GGGAC 2 cut(s) 722, 961
BsmI GAATGC 1 cut(s) 104
BsnI GGCC 2 cut(s) 269, 828
BsoBI CYCGRG 1 cut(s) 967
Bsp1286I GDGCHC 2 cut(s) 234, 543
Bsp143I GATC 7 cut(s) 352, 404, 451, 549, 607, 883, 888
BspACI CCGC 1 cut(s) 972
BspANI GGCC 2 cut(s) 269, 828
BspCNI CTCAG 3 cut(s) 736, 990, 1157
BspLI GGNNCC 5 cut(s) 297, 406, 976, 977, 991
BspMAI CTGCAG 3 cut(s) 111, 363, 640
BspMI ACCTGC 2 cut(s) 352, 366
BspPI GGATC 4 cut(s) 399, 412, 602, 883
BspT107I GGYRCC 2 cut(s) 295, 989
BsrBI CCGCTC 1 cut(s) 972
BsrI ACTGG 2 cut(s) 93, 544
BssMI GATC 7 cut(s) 352, 404, 451, 549, 607, 883, 888
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 2 cut(s) 522, 910
BstC8I GCNNGC 4 cut(s) 254, 426, 1136, 1167
BstDEI CTNAG 8 cut(s) 397, 416, 615, 744, 893, 980, 998, 1144
BstENI CCTNNNNNAGG 1 cut(s) 983
BstF5I GGATG 3 cut(s) 645, 807, 1138
BstKTI GATC 7 cut(s) 355, 407, 454, 552, 610, 886, 891
BstMAI GTCTC 1 cut(s) 1059
BstMBI GATC 7 cut(s) 352, 404, 451, 549, 607, 883, 888
BstMWI GCNNNNNNNGC 4 cut(s) 196, 629, 638, 650
BstNI CCWGG 1 cut(s) 650
BstNSI RCATGY 4 cut(s) 256, 428, 1092, 1138
BstSCI CCNGG 1 cut(s) 648
BstSFI CTRYAG 4 cut(s) 107, 359, 531, 636
BstSLI GKGCMC 1 cut(s) 543
BstV1I GCAGC 2 cut(s) 628, 650
BstV2I GAAGAC 3 cut(s) 390, 824, 1024
BstX2I RGATCY 4 cut(s) 404, 451, 607, 888
BstYI RGATCY 4 cut(s) 404, 451, 607, 888
BsuRI GGCC 2 cut(s) 269, 828
BtsCI GGATG 3 cut(s) 645, 807, 1138
BtsIMutI CAGTG 3 cut(s) 100, 527, 1074
BveI ACCTGC 2 cut(s) 352, 366
Cac8I GCNNGC 4 cut(s) 254, 426, 1136, 1167
Cfr13I GGNCC 1 cut(s) 975
Csp6I GTAC 6 cut(s) 518, 558, 672, 755, 903, 990
CviAII CATG 7 cut(s) 253, 281, 337, 425, 1089, 1103, 1135
CviQI GTAC 6 cut(s) 518, 558, 672, 755, 903, 990
DdeI CTNAG 8 cut(s) 397, 416, 615, 744, 893, 980, 998, 1144
DpnI GATC 7 cut(s) 354, 406, 453, 551, 609, 885, 890
DpnII GATC 7 cut(s) 352, 404, 451, 549, 607, 883, 888
Ecl136II GAGCTC 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 234
Eco47I GGWCC 1 cut(s) 975
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 2 cut(s) 402, 1050
Eco88I CYCGRG 1 cut(s) 967
EcoICRI GAGCTC 1 cut(s) 232
EcoNI CCTNNNNNAGG 1 cut(s) 983
EcoO109I RGGNCCY 1 cut(s) 975
EcoRI GAATTC 1 cut(s) 1007
EcoRII CCWGG 1 cut(s) 648
EcoT22I ATGCAT 2 cut(s) 426, 1140
EcoT38I GRGCYC 1 cut(s) 234
FaeI CATG 7 cut(s) 256, 284, 340, 428, 1092, 1106, 1138
FaqI GGGAC 2 cut(s) 722, 961
FatI CATG 7 cut(s) 252, 280, 336, 424, 1088, 1102, 1134
FauI CCCGC 1 cut(s) 965
FauNDI CATATG 2 cut(s) 24, 823
FbaI TGATCA 2 cut(s) 549, 883
FblI GTMKAC 1 cut(s) 792
Fnu4HI GCNGC 2 cut(s) 639, 642
FokI GGATG 3 cut(s) 632, 794, 1125
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 2 cut(s) 639, 642
FspBI CTAG 5 cut(s) 449, 515, 683, 830, 1058
GluI GCNGC 2 cut(s) 639, 642
HaeIII GGCC 2 cut(s) 269, 828
Hin1II CATG 7 cut(s) 256, 284, 340, 428, 1092, 1106, 1138
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 4 cut(s) 86, 112, 944, 1109
HphI GGTGA 3 cut(s) 156, 347, 965
Hpy166II GTNNAC 3 cut(s) 772, 793, 903
Hpy188I TCNGA 8 cut(s) 409, 700, 726, 849, 888, 999, 1013, 1036
Hpy188III TCNNGA 4 cut(s) 70, 242, 449, 1058
Hpy8I GTNNAC 3 cut(s) 772, 793, 903
Hpy99I CGWCG 1 cut(s) 602
HpyAV CCTTC 4 cut(s) 67, 113, 731, 1109
HpyCH4III ACNGT 2 cut(s) 522, 910
HpyCH4IV ACGT 2 cut(s) 774, 798
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 629, 638, 650
HpyF3I CTNAG 8 cut(s) 397, 416, 615, 744, 893, 980, 998, 1144
HpySE526I ACGT 2 cut(s) 774, 798
Hsp92II CATG 7 cut(s) 256, 284, 340, 428, 1092, 1106, 1138
KflI GGGWCCC 1 cut(s) 975
KpnI GGTACC 1 cut(s) 993
Ksp22I TGATCA 2 cut(s) 549, 883
Kzo9I GATC 7 cut(s) 352, 404, 451, 549, 607, 883, 888
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 2 cut(s) 628, 650
LweI GCATC 2 cut(s) 641, 1147
MaeI CTAG 5 cut(s) 449, 515, 683, 830, 1058
MaeII ACGT 2 cut(s) 774, 798
MaeIII GTNAC 1 cut(s) 162
MalI GATC 7 cut(s) 354, 406, 453, 551, 609, 885, 890
MbiI CCGCTC 1 cut(s) 972
MboI GATC 7 cut(s) 352, 404, 451, 549, 607, 883, 888
MboII GAAGA 8 cut(s) 81, 230, 395, 488, 795, 829, 1026, 1029
MfeI CAATTG 1 cut(s) 1157
MflI RGATCY 4 cut(s) 404, 451, 607, 888
MhlI GDGCHC 2 cut(s) 234, 543
MmeI TCCRAC 1 cut(s) 442
Mph1103I ATGCAT 2 cut(s) 426, 1140
MroXI GAANNNNTTC 1 cut(s) 102
MseI TTAA 7 cut(s) 572, 627, 692, 854, 935, 1116, 1172
MslI CAYNNNNRTG 1 cut(s) 27
MspA1I CMGCKG 2 cut(s) 623, 641
MspR9I CCNGG 1 cut(s) 650
MunI CAATTG 1 cut(s) 1157
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 1 cut(s) 650
MwoI GCNNNNNNNGC 4 cut(s) 196, 629, 638, 650
NdeI CATATG 2 cut(s) 24, 823
NdeII GATC 7 cut(s) 352, 404, 451, 549, 607, 883, 888
NlaIII CATG 7 cut(s) 256, 284, 340, 428, 1092, 1106, 1138
NlaIV GGNNCC 5 cut(s) 297, 406, 976, 977, 991
NmuCI GTSAC 1 cut(s) 162
NsiI ATGCAT 2 cut(s) 426, 1140
NspI RCATGY 4 cut(s) 256, 428, 1092, 1138
PaeI GCATGC 3 cut(s) 256, 428, 1138
PaeR7I CTCGAG 1 cut(s) 967
PaqCI CACCTGC 1 cut(s) 366
PciI ACATGT 1 cut(s) 1088
PcsI WCGNNNNNNNCGW 1 cut(s) 722
PctI GAATGC 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 102
PfeI GAWTC 4 cut(s) 86, 112, 944, 1109
PkrI GCNGC 2 cut(s) 640, 643
PpuMI RGGWCCY 1 cut(s) 975
PscI ACATGT 1 cut(s) 1088
PshBI ATTAAT 1 cut(s) 572
Psp124BI GAGCTC 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 975
Psp6I CCWGG 1 cut(s) 648
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 5 cut(s) 297, 406, 976, 977, 991
PspPI GGNCC 1 cut(s) 975
PspPPI RGGWCCY 1 cut(s) 975
PspXI VCTCGAGB 1 cut(s) 967
PstI CTGCAG 3 cut(s) 111, 363, 640
PsuI RGATCY 4 cut(s) 404, 451, 607, 888
PvuII CAGCTG 2 cut(s) 623, 641
RsaI GTAC 6 cut(s) 519, 559, 673, 756, 904, 991
RsaNI GTAC 6 cut(s) 518, 558, 672, 755, 903, 990
RseI CAYNNNNRTG 1 cut(s) 27
SacI GAGCTC 1 cut(s) 234
SaqAI TTAA 7 cut(s) 572, 627, 692, 854, 935, 1116, 1172
SatI GCNGC 2 cut(s) 639, 642
Sau3AI GATC 7 cut(s) 352, 404, 451, 549, 607, 883, 888
Sau96I GGNCC 1 cut(s) 975
SbfI CCTGCAGG 1 cut(s) 363
ScrFI CCNGG 1 cut(s) 650
SdaI CCTGCAGG 1 cut(s) 363
SduI GDGCHC 2 cut(s) 234, 543
SfaNI GCATC 2 cut(s) 641, 1147
SfcI CTRYAG 4 cut(s) 107, 359, 531, 636
Sfr274I CTCGAG 1 cut(s) 967
SinI GGWCC 1 cut(s) 975
SlaI CTCGAG 1 cut(s) 967
SmiMI CAYNNNNRTG 1 cut(s) 27
SmlI CTYRAG 4 cut(s) 242, 864, 967, 1025
SmoI CTYRAG 4 cut(s) 242, 864, 967, 1025
SphI GCATGC 3 cut(s) 256, 428, 1138
Sse8387I CCTGCAGG 1 cut(s) 363
SsiI CCGC 1 cut(s) 972
SspMI CTAG 5 cut(s) 449, 515, 683, 830, 1058
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 648
TaaI ACNGT 2 cut(s) 522, 910
TaiI ACGT 2 cut(s) 777, 801
TaqI TCGA 4 cut(s) 351, 372, 947, 968
TatI WGTACW 3 cut(s) 517, 557, 754
TfiI GAWTC 4 cut(s) 86, 112, 944, 1109
Tru1I TTAA 7 cut(s) 572, 627, 692, 854, 935, 1116, 1172
Tru9I TTAA 7 cut(s) 572, 627, 692, 854, 935, 1116, 1172
TscAI CASTG 3 cut(s) 100, 527, 1074
TseFI GTSAC 1 cut(s) 162
TseI GCWGC 2 cut(s) 638, 641
Tsp45I GTSAC 1 cut(s) 162
TspDTI ATGAA 1 cut(s) 1091
TspRI CASTG 3 cut(s) 100, 527, 1074
VpaK11BI GGWCC 1 cut(s) 975
VspI ATTAAT 1 cut(s) 572
XagI CCTNNNNNAGG 1 cut(s) 983
XapI RAATTY 3 cut(s) 410, 444, 1007
XbaI TCTAGA 2 cut(s) 448, 1057
XceI RCATGY 4 cut(s) 256, 428, 1092, 1138
XhoI CTCGAG 1 cut(s) 967
XmiI GTMKAC 1 cut(s) 792
XmnI GAANNNNTTC 1 cut(s) 102
XspI CTAG 5 cut(s) 449, 515, 683, 830, 1058
Zsp2I ATGCAT 2 cut(s) 426, 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.