pycom04g00090

Importin-beta N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0003769
Physical Location & Seq
Forward (+)
33448 .. 33958
511 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00090.1

Sequence Viewer

Length: 309 bp
ATGCTTATTTGCACATGCAGTGTGTGTACGACTGTAATTTTGGGTGGGAACGATGACTTAACAGAGGAATCGAGTGGTGATAACATAAGCTCGAGCGGGTCCCTTAGCAAGGTTACCATTCTGAGTAAAGAATTCAGAAGAAGACAGCTCAAGGTTTCAGACCCTATCAACCAAATGTCTCTAGAGGCTTCAGTGAGGGAAAATCTCCAAACATGTGCTACCTTTCATGGAGAATCTTTTAATAAGGCGATTGGTTGCATGCATCCCAAAGCATTTTCACAATTGAAGCAGGCATTAAAGATGGAGTAG

Protein Analysis

103

Amino Acids

11.14

Weight (kDa)

6.71

Isoelectric Point (pI)

71.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_IPO11 PF25758 6 - 99 1.9e-09 Importin-11-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 96
AciI CCGC 1 cut(s) 96
AcsI RAATTY 1 cut(s) 131
AcuI CTGAAG 1 cut(s) 174
AfaI GTAC 1 cut(s) 28
AfiI CCNNNNNNNGG 1 cut(s) 109
AflIII ACRYGT 1 cut(s) 212
AgsI TTSAA 1 cut(s) 286
AluBI AGCT 2 cut(s) 90, 148
AluI AGCT 2 cut(s) 90, 148
Alw26I GTCTC 1 cut(s) 183
Ama87I CYCGRG 1 cut(s) 91
ApoI RAATTY 1 cut(s) 131
ArsI GACNNNNNNTTYG 2 cut(s) 22, 54
AspS9I GGNCC 1 cut(s) 99
AsuHPI GGTGA 1 cut(s) 89
AvaI CYCGRG 1 cut(s) 91
AvaII GGWCC 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 148
BccI CCATC 1 cut(s) 295
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 1 cut(s) 182
Bme18I GGWCC 1 cut(s) 99
BmeT110I CYCGRG 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 99
BmiI GGNNCC 2 cut(s) 100, 101
BmsI GCATC 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 148
Bpu10I CCTNAGC 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 134
Bsc4I CCNNNNNNNGG 1 cut(s) 109
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 1 cut(s) 109
BseMII CTCAG 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 91
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 1 cut(s) 109
BsmAI GTCTC 1 cut(s) 183
BsmFI GGGAC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 91
BspACI CCGC 1 cut(s) 96
BspCNI CTCAG 1 cut(s) 114
BspLI GGNNCC 2 cut(s) 100, 101
BsrBI CCGCTC 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 260, 291
BstDEI CTNAG 2 cut(s) 104, 122
BstEII GGTNACC 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 1 cut(s) 262
BstMAI GTCTC 1 cut(s) 183
BstNSI RCATGY 3 cut(s) 18, 216, 262
BstPI GGTNACC 1 cut(s) 112
BstV2I GAAGAC 1 cut(s) 148
BtsCI GGATG 1 cut(s) 262
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 198
Cac8I GCNNGC 2 cut(s) 260, 291
Cfr13I GGNCC 1 cut(s) 99
Csp6I GTAC 1 cut(s) 27
CviAII CATG 4 cut(s) 15, 213, 227, 259
CviJI RGCY 3 cut(s) 90, 148, 188
CviKI_1 RGCY 3 cut(s) 90, 148, 188
CviQI GTAC 1 cut(s) 27
DdeI CTNAG 2 cut(s) 104, 122
Eco47I GGWCC 1 cut(s) 99
Eco57I CTGAAG 1 cut(s) 174
Eco88I CYCGRG 1 cut(s) 91
Eco91I GGTNACC 1 cut(s) 112
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 99
EcoO65I GGTNACC 1 cut(s) 112
EcoRI GAATTC 1 cut(s) 131
EcoT22I ATGCAT 1 cut(s) 264
FaeI CATG 4 cut(s) 18, 216, 230, 262
FaiI YATR 5 cut(s) 16, 86, 214, 228, 260
FaqI GGGAC 1 cut(s) 85
FatI CATG 4 cut(s) 14, 212, 226, 258
FauI CCCGC 1 cut(s) 89
FokI GGATG 1 cut(s) 249
FspBI CTAG 1 cut(s) 182
Hin1II CATG 4 cut(s) 18, 216, 230, 262
HinfI GANTC 2 cut(s) 68, 233
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 3 cut(s) 123, 137, 160
Hpy188III TCNNGA 1 cut(s) 182
Hpy8I GTNNAC 1 cut(s) 27
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4V TGCA 4 cut(s) 12, 18, 258, 262
HpyF3I CTNAG 2 cut(s) 104, 122
Hsp92II CATG 4 cut(s) 18, 216, 230, 262
KflI GGGWCCC 1 cut(s) 99
LpnPI CCDG 1 cut(s) 275
LweI GCATC 1 cut(s) 271
MaeI CTAG 1 cut(s) 182
MaeIII GTNAC 1 cut(s) 112
MbiI CCGCTC 1 cut(s) 96
MboII GAAGA 2 cut(s) 150, 153
MfeI CAATTG 1 cut(s) 281
MluCI AATT 3 cut(s) 36, 131, 281
MnlI CCTC 3 cut(s) 58, 178, 189
Mph1103I ATGCAT 1 cut(s) 264
MseI TTAA 3 cut(s) 59, 240, 296
MunI CAATTG 1 cut(s) 281
NlaIII CATG 4 cut(s) 18, 216, 230, 262
NlaIV GGNNCC 2 cut(s) 100, 101
NsiI ATGCAT 1 cut(s) 264
NspI RCATGY 3 cut(s) 18, 216, 262
PaeI GCATGC 1 cut(s) 262
PaeR7I CTCGAG 1 cut(s) 91
PciI ACATGT 1 cut(s) 212
PfeI GAWTC 2 cut(s) 68, 233
PpuMI RGGWCCY 1 cut(s) 99
PscI ACATGT 1 cut(s) 212
Psp5II RGGWCCY 1 cut(s) 99
PspEI GGTNACC 1 cut(s) 112
PspN4I GGNNCC 2 cut(s) 100, 101
PspPI GGNCC 1 cut(s) 99
PspPPI RGGWCCY 1 cut(s) 99
PspXI VCTCGAGB 1 cut(s) 91
RsaI GTAC 1 cut(s) 28
RsaNI GTAC 1 cut(s) 27
SaqAI TTAA 3 cut(s) 59, 240, 296
Sau96I GGNCC 1 cut(s) 99
SetI ASST 5 cut(s) 92, 114, 150, 156, 224
SfaNI GCATC 1 cut(s) 271
Sfr274I CTCGAG 1 cut(s) 91
SinI GGWCC 1 cut(s) 99
SlaI CTCGAG 1 cut(s) 91
SmlI CTYRAG 2 cut(s) 91, 149
SmoI CTYRAG 2 cut(s) 91, 149
SphI GCATGC 1 cut(s) 262
Sse9I AATT 3 cut(s) 36, 131, 281
SsiI CCGC 1 cut(s) 96
SspMI CTAG 1 cut(s) 182
TaaI ACNGT 1 cut(s) 34
TaqI TCGA 2 cut(s) 71, 92
TasI AATT 3 cut(s) 36, 131, 281
TfiI GAWTC 2 cut(s) 68, 233
Tru1I TTAA 3 cut(s) 59, 240, 296
Tru9I TTAA 3 cut(s) 59, 240, 296
TscAI CASTG 2 cut(s) 25, 198
TspDTI ATGAA 1 cut(s) 215
TspRI CASTG 2 cut(s) 25, 198
VpaK11BI GGWCC 1 cut(s) 99
XagI CCTNNNNNAGG 1 cut(s) 107
XapI RAATTY 1 cut(s) 131
XbaI TCTAGA 1 cut(s) 181
XceI RCATGY 3 cut(s) 18, 216, 262
XhoI CTCGAG 1 cut(s) 91
XspI CTAG 1 cut(s) 182
Zsp2I ATGCAT 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.