MD04G1050900.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005315
Physical Location & Seq
Forward (+)
5985584 .. 5989680
4097 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1050900.v1.1.491

Sequence Viewer

Length: 864 bp
ATGAGAGAGGGCCAAGTGGGGGGAATCCGAATCCAGAATCCGTTTACTTTGAAAGTGGGTCAAGTATTTACAGGCTTTGGCGTAGGCTGCGGTGTTGGTATCGGAGTTGGCCGCCCTATAAATCTAGGTGCAATACCAGCACTGAATCAAGTCATGGGTGCTACAAGAGGTGCCACTGATGCATTATCTGGTGTTAGCAGGCATGTAAACAACACTTTGAGGAAGGTGGGAGCCAAGAGCATTGAAGCCGGCATTGGATGTGGAGTTGGTATTGGCCATGGTTTTGGAGTTGGCCTTGCTCCGATGCCAGGAGTGTTGCAGCAAATTCAATTTCGTTTTGTACAAGGAATGACAAAGATGATGATGAAGTTTGGAATTTTTCCCAATTTACCCATTGGTCAAGGTGCCCTCCCAAGTCCTTTGCAAAGTGCCATGGGAACTTTAAATGACCTGAGCCAACATAAAATGGGAAGTAACACGCAGTTGTCAACAAAACTAACAGACTACGCATCTCAAGGCTTGCCTGGATGTGGACATAACGGTGCTGGTTCTCCAGTTGATACACCTTTTGGTAGTCGAATAGAAAATGTTCTCAGCAGTTTCCAGAATCCACTTCTGAAAGGAAAGGACAGTGAACAGAATGAAGGGGCTGGACGCGTGAGGTCGGAGAATAAAATCCTTCAAATGATATTGAAACACCAGCAAGTCATTGAAGAGCTCATGGAGGAGAACGAGAAGCTTCGCCAAGTACTAGTGGAAGACCTGAAAGTATCACCTAGCAAGCTGCAAGCAAGTTATTCAAGTAAAAACACATCTCCGTGTACAGATTGTTTCGAGTGCCGAAGAAAACAAAGGAGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

288

Amino Acids

30.6

Weight (kDa)

9.66

Isoelectric Point (pI)

49.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 170, 404
AccII CGCG 1 cut(s) 657
AciI CCGC 2 cut(s) 90, 112
AcoI YGGCCR 2 cut(s) 109, 274
AcsI RAATTY 2 cut(s) 324, 375
AfaI GTAC 3 cut(s) 342, 750, 823
AfiI CCNNNNNNNGG 3 cut(s) 19, 308, 530
AflIII ACRYGT 1 cut(s) 655
AgsI TTSAA 7 cut(s) 52, 245, 329, 683, 694, 713, 801
AhlI ACTAGT 1 cut(s) 751
AjnI CCWGG 2 cut(s) 307, 523
AjuI GAANNNNNNNTTGG 2 cut(s) 237, 269
AluBI AGCT 3 cut(s) 718, 739, 784
AluI AGCT 3 cut(s) 718, 739, 784
Alw21I GWGCWC 1 cut(s) 720
AoxI GGCC 4 cut(s) 10, 109, 274, 292
ApeKI GCWGC 3 cut(s) 87, 319, 784
ApoI RAATTY 2 cut(s) 324, 375
Asp700I GAANNNNTTC 1 cut(s) 588
AspS9I GGNCC 1 cut(s) 10
AsuHPI GGTGA 1 cut(s) 765
BaeGI GKGCMC 1 cut(s) 409
BalI TGGCCA 1 cut(s) 276
BanI GGYRCC 2 cut(s) 170, 404
BanII GRGCYC 1 cut(s) 720
BbsI GAAGAC 1 cut(s) 765
Bbv12I GWGCWC 1 cut(s) 720
BbvI GCAGC 3 cut(s) 74, 331, 771
BciT130I CCWGG 2 cut(s) 309, 525
BcuI ACTAGT 1 cut(s) 751
BfaI CTAG 3 cut(s) 125, 752, 777
BisI GCNGC 4 cut(s) 88, 112, 320, 785
BlsI GCNGC 4 cut(s) 89, 113, 321, 786
BmcAI AGTACT 1 cut(s) 750
Bme1390I CCNGG 2 cut(s) 309, 525
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 3 cut(s) 172, 232, 406
BmrFI CCNGG 2 cut(s) 309, 525
BmsI GCATC 3 cut(s) 169, 294, 518
BpiI GAAGAC 1 cut(s) 765
BpmI CTGGAG 1 cut(s) 537
Bpu10I CCTNAGC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 498
BsaJI CCNNGG 2 cut(s) 277, 432
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 308, 530
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 554
BseBI CCWGG 2 cut(s) 309, 525
BseDI CCNNGG 2 cut(s) 277, 432
BseGI GGATG 2 cut(s) 263, 533
BseLI CCNNNNNNNGG 3 cut(s) 19, 308, 530
BseMII CTCAG 2 cut(s) 443, 607
BseNI ACTGG 1 cut(s) 554
BseRI GAGGAG 1 cut(s) 740
BseSI GKGCMC 1 cut(s) 409
BseXI GCAGC 3 cut(s) 74, 331, 771
Bsh1236I CGCG 1 cut(s) 657
BshFI GGCC 4 cut(s) 12, 111, 276, 294
BshNI GGYRCC 2 cut(s) 170, 404
BsiHKAI GWGCWC 1 cut(s) 720
BsiSI CCGG 1 cut(s) 249
BslI CCNNNNNNNGG 3 cut(s) 19, 308, 530
BsnI GGCC 4 cut(s) 12, 111, 276, 294
Bsp1286I GDGCHC 2 cut(s) 409, 720
Bsp1407I TGTACA 2 cut(s) 340, 821
Bsp19I CCATGG 2 cut(s) 277, 432
BspACI CCGC 2 cut(s) 90, 112
BspANI GGCC 4 cut(s) 12, 111, 276, 294
BspCNI CTCAG 2 cut(s) 444, 606
BspFNI CGCG 1 cut(s) 657
BspLI GGNNCC 3 cut(s) 172, 232, 406
BspQI GCTCTTC 1 cut(s) 708
BspT107I GGYRCC 2 cut(s) 170, 404
BsrFI RCCGGY 1 cut(s) 248
BsrGI TGTACA 2 cut(s) 340, 821
BsrI ACTGG 1 cut(s) 554
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 277, 432
BssT1I CCWWGG 2 cut(s) 277, 432
Bst2UI CCWGG 2 cut(s) 309, 525
Bst4CI ACNGT 2 cut(s) 542, 632
Bst6I CTCTTC 1 cut(s) 708
BstAUI TGTACA 2 cut(s) 340, 821
BstC8I GCNNGC 5 cut(s) 200, 250, 521, 782, 789
BstDEI CTNAG 2 cut(s) 452, 593
BstDSI CCRYGG 2 cut(s) 277, 432
BstF5I GGATG 2 cut(s) 263, 533
BstFNI CGCG 1 cut(s) 657
BstMWI GCNNNNNNNGC 3 cut(s) 87, 137, 179
BstNI CCWGG 2 cut(s) 309, 525
BstNSI RCATGY 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 307, 523
BstSLI GKGCMC 1 cut(s) 409
BstUI CGCG 1 cut(s) 657
BstV1I GCAGC 3 cut(s) 74, 331, 771
BstV2I GAAGAC 1 cut(s) 765
BstXI CCANNNNNNTGG 1 cut(s) 284
BsuRI GGCC 4 cut(s) 12, 111, 276, 294
BtgI CCRYGG 2 cut(s) 277, 432
BtsCI GGATG 2 cut(s) 263, 533
BtsIMutI CAGTG 3 cut(s) 140, 174, 637
Cac8I GCNNGC 5 cut(s) 200, 250, 521, 782, 789
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 10
CseI GACGC 1 cut(s) 663
Csp6I GTAC 3 cut(s) 341, 749, 822
CviAII CATG 5 cut(s) 154, 203, 278, 433, 721
CviQI GTAC 3 cut(s) 341, 749, 822
DdeI CTNAG 2 cut(s) 452, 593
DraI TTTAAA 1 cut(s) 444
EaeI YGGCCR 2 cut(s) 109, 274
Eam1104I CTCTTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 708
Ecl136II GAGCTC 1 cut(s) 718
Eco130I CCWWGG 2 cut(s) 277, 432
Eco24I GRGCYC 1 cut(s) 720
Eco53kI GAGCTC 1 cut(s) 718
EcoICRI GAGCTC 1 cut(s) 718
EcoRII CCWGG 2 cut(s) 307, 523
EcoT14I CCWWGG 2 cut(s) 277, 432
EcoT22I ATGCAT 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 720
ErhI CCWWGG 2 cut(s) 277, 432
FaeI CATG 5 cut(s) 157, 206, 281, 436, 724
FaiI YATR 8 cut(s) 119, 155, 204, 279, 434, 462, 537, 722
FatI CATG 5 cut(s) 153, 202, 277, 432, 720
Fnu4HI GCNGC 4 cut(s) 88, 112, 320, 785
FokI GGATG 2 cut(s) 270, 540
FriOI GRGCYC 1 cut(s) 720
Fsp4HI GCNGC 4 cut(s) 88, 112, 320, 785
FspBI CTAG 3 cut(s) 125, 752, 777
GluI GCNGC 4 cut(s) 88, 112, 320, 785
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 4 cut(s) 12, 111, 276, 294
HapII CCGG 1 cut(s) 249
HgaI GACGC 1 cut(s) 663
Hin1II CATG 5 cut(s) 157, 206, 281, 436, 724
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 5 cut(s) 24, 30, 37, 145, 607
HpaII CCGG 1 cut(s) 249
HphI GGTGA 1 cut(s) 765
Hpy166II GTNNAC 6 cut(s) 45, 208, 489, 533, 635, 822
Hpy188I TCNGA 5 cut(s) 29, 104, 303, 618, 667
Hpy188III TCNNGA 2 cut(s) 34, 604
Hpy8I GTNNAC 6 cut(s) 45, 208, 489, 533, 635, 822
HpyAV CCTTC 3 cut(s) 217, 638, 689
HpyCH4III ACNGT 2 cut(s) 542, 632
HpyCH4V TGCA 5 cut(s) 131, 182, 319, 424, 787
HpyF10VI GCNNNNNNNGC 3 cut(s) 87, 137, 179
HpyF3I CTNAG 2 cut(s) 452, 593
Hsp92II CATG 5 cut(s) 157, 206, 281, 436, 724
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
LguI GCTCTTC 1 cut(s) 708
LmnI GCTCC 2 cut(s) 230, 304
Lsp1109I GCAGC 3 cut(s) 74, 331, 771
LweI GCATC 3 cut(s) 169, 294, 518
MaeI CTAG 3 cut(s) 125, 752, 777
MaeIII GTNAC 1 cut(s) 473
MboII GAAGA 3 cut(s) 725, 770, 855
MhlI GDGCHC 2 cut(s) 409, 720
MlsI TGGCCA 1 cut(s) 276
MluCI AATT 4 cut(s) 324, 329, 375, 385
MluI ACGCGT 1 cut(s) 655
MluNI TGGCCA 1 cut(s) 276
MmeI TCCRAC 1 cut(s) 645
MnlI CCTC 5 cut(s) 161, 213, 419, 654, 718
Mox20I TGGCCA 1 cut(s) 276
Mph1103I ATGCAT 1 cut(s) 184
MroNI GCCGGC 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 588
MscI TGGCCA 1 cut(s) 276
MseI TTAA 1 cut(s) 443
MslI CAYNNNNRTG 2 cut(s) 540, 817
Msp20I TGGCCA 1 cut(s) 276
MspI CCGG 1 cut(s) 249
MspR9I CCNGG 2 cut(s) 309, 525
MvaI CCWGG 2 cut(s) 309, 525
MvnI CGCG 1 cut(s) 657
MwoI GCNNNNNNNGC 3 cut(s) 87, 137, 179
NaeI GCCGGC 1 cut(s) 250
NcoI CCATGG 2 cut(s) 277, 432
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 5 cut(s) 157, 206, 281, 436, 724
NlaIV GGNNCC 3 cut(s) 172, 232, 406
NsiI ATGCAT 1 cut(s) 184
NspI RCATGY 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 708
PdiI GCCGGC 1 cut(s) 250
PdmI GAANNNNTTC 1 cut(s) 588
PfeI GAWTC 5 cut(s) 24, 30, 37, 145, 607
PkrI GCNGC 4 cut(s) 89, 113, 321, 786
Psp124BI GAGCTC 1 cut(s) 720
Psp6I CCWGG 2 cut(s) 307, 523
PspGI CCWGG 2 cut(s) 307, 523
PspN4I GGNNCC 3 cut(s) 172, 232, 406
PspPI GGNCC 1 cut(s) 10
RsaI GTAC 3 cut(s) 342, 750, 823
RsaNI GTAC 3 cut(s) 341, 749, 822
RseI CAYNNNNRTG 2 cut(s) 540, 817
SacI GAGCTC 1 cut(s) 720
SapI GCTCTTC 1 cut(s) 708
SaqAI TTAA 1 cut(s) 443
SatI GCNGC 4 cut(s) 88, 112, 320, 785
Sau96I GGNCC 1 cut(s) 10
ScaI AGTACT 1 cut(s) 750
ScrFI CCNGG 2 cut(s) 309, 525
SduI GDGCHC 2 cut(s) 409, 720
SfaNI GCATC 3 cut(s) 169, 294, 518
SmiMI CAYNNNNRTG 2 cut(s) 540, 817
SmlI CTYRAG 1 cut(s) 513
SmoI CTYRAG 1 cut(s) 513
SpeI ACTAGT 1 cut(s) 751
Sse9I AATT 4 cut(s) 324, 329, 375, 385
SsiI CCGC 2 cut(s) 90, 112
SspMI CTAG 3 cut(s) 125, 752, 777
SstI GAGCTC 1 cut(s) 720
StyD4I CCNGG 2 cut(s) 307, 523
StyI CCWWGG 2 cut(s) 277, 432
TaaI ACNGT 2 cut(s) 542, 632
TaqI TCGA 2 cut(s) 577, 834
TasI AATT 4 cut(s) 324, 329, 375, 385
TatI WGTACW 3 cut(s) 340, 748, 821
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 5 cut(s) 24, 30, 37, 145, 607
Tru1I TTAA 1 cut(s) 443
Tru9I TTAA 1 cut(s) 443
TscAI CASTG 3 cut(s) 147, 181, 637
TseI GCWGC 3 cut(s) 87, 319, 784
TspDTI ATGAA 2 cut(s) 380, 657
TspGWI ACGGA 2 cut(s) 30, 807
TspRI CASTG 3 cut(s) 147, 181, 637
XapI RAATTY 2 cut(s) 324, 375
XceI RCATGY 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 588
XspI CTAG 3 cut(s) 125, 752, 777
ZrmI AGTACT 1 cut(s) 750
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.