pycom04g04500

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005315
Physical Location & Seq
Forward (+)
4088118 .. 4091055
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g04500.1

Sequence Viewer

Length: 864 bp
ATGAGAGAAGGCCAAGTGGGGGGAATCCGAATCCAGAATCCGTTTACTTTGAAAGTGGGTCAAGTATTTACAGGCTTTGGCGTAGGCTGCGGTGTTGGTATCGGAGTTGGCCGCCCTATAAATCTAGGTGCAGTACCAGCACTGAATCAAATCATGGGTGCTACAAGAGGTGCCACTGATGCATTATCTGGTGTTAGCAGGCATGTAAACAACACTTTGAGGAAGGTGGGAGCCAAGAACATTGAAGCAGGGATTGGATGTGGAGTTGGTATTGGCCATGGTTTTGGAGTTGGCCTTGCTCCGAAGCCAGGAGTGTTGCAGCAAATTCAATTTCGTTTTGTACAAGGAATGACAAAGATGATGATGAAGTTTGGAATTTTTCCCAATATACCCATTGGTCAAGGTGCCCTCCCAAGTTCCTTGCAAAGTGCCATGGGAACTTTAAATGACCGGAGCCAAAATAAAATGGGAAGTAACACGCAGTTGTCAACAAAACTAACAGACTACGCATCTCAAGGCTTGCCTGGATATGGACGTAATGGTGCTGGTTCTCCAGTTGATACACCTTTTGGCAGTCGAATAGAAAATGTTCCCAGCAGTTTTCAGAATCCACTTCTGAAGGGAAAGGACAGTGAACAGAATGAAGGGGCTGGACGCGTGAGGTCGGAGAAAACAATCCTTCAAATGATATTGAAACACCAGCAAGTCATTGAAGAGCTCGCGGAGGAGAACGAGAAGCTTCGCCAAGTACTAGTGGAAGACCTGAAAGTATCACCTAGCAAGCTGCAAGCAAGTTATTCAATTAAAAACACATCTCCGTGTACAGATTGTTTCAAGTGTCGAAGAAAACAAGGGAGAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

288

Amino Acids

30.55

Weight (kDa)

9.95

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 170, 404
AccII CGCG 2 cut(s) 657, 722
AciI CCGC 3 cut(s) 90, 112, 722
AcoI YGGCCR 2 cut(s) 109, 274
AcsI RAATTY 2 cut(s) 324, 375
AcuI CTGAAG 1 cut(s) 638
AfaI GTAC 4 cut(s) 135, 342, 750, 823
AfiI CCNNNNNNNGG 3 cut(s) 19, 308, 530
AflIII ACRYGT 1 cut(s) 655
AgsI TTSAA 8 cut(s) 52, 245, 329, 683, 694, 713, 801, 835
AhlI ACTAGT 1 cut(s) 751
AjnI CCWGG 2 cut(s) 307, 523
AjuI GAANNNNNNNTTGG 2 cut(s) 237, 269
AluBI AGCT 3 cut(s) 718, 739, 784
AluI AGCT 3 cut(s) 718, 739, 784
Alw21I GWGCWC 1 cut(s) 720
AoxI GGCC 4 cut(s) 10, 109, 274, 292
ApeKI GCWGC 3 cut(s) 87, 319, 784
ApoI RAATTY 2 cut(s) 324, 375
Asp700I GAANNNNTTC 1 cut(s) 588
AsuHPI GGTGA 1 cut(s) 765
BaeGI GKGCMC 1 cut(s) 409
BalI TGGCCA 1 cut(s) 276
BanI GGYRCC 2 cut(s) 170, 404
BanII GRGCYC 1 cut(s) 720
BbsI GAAGAC 1 cut(s) 765
Bbv12I GWGCWC 1 cut(s) 720
BbvI GCAGC 3 cut(s) 74, 331, 771
BciT130I CCWGG 2 cut(s) 309, 525
BcuI ACTAGT 1 cut(s) 751
BfaI CTAG 3 cut(s) 125, 752, 777
BisI GCNGC 4 cut(s) 88, 112, 320, 785
BlsI GCNGC 4 cut(s) 89, 113, 321, 786
BmcAI AGTACT 1 cut(s) 750
Bme1390I CCNGG 2 cut(s) 309, 525
BmiI GGNNCC 4 cut(s) 172, 232, 406, 455
BmrFI CCNGG 2 cut(s) 309, 525
BmsI GCATC 2 cut(s) 169, 518
BpiI GAAGAC 1 cut(s) 765
BpmI CTGGAG 1 cut(s) 537
BpuEI CTTGAG 1 cut(s) 498
BsaJI CCNNGG 2 cut(s) 277, 432
BsaWI WCCGGW 1 cut(s) 450
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 308, 530
Bse1I ACTGG 1 cut(s) 554
BseBI CCWGG 2 cut(s) 309, 525
BseDI CCNNGG 2 cut(s) 277, 432
BseGI GGATG 1 cut(s) 263
BseLI CCNNNNNNNGG 3 cut(s) 19, 308, 530
BseNI ACTGG 1 cut(s) 554
BseRI GAGGAG 1 cut(s) 740
BseSI GKGCMC 1 cut(s) 409
BseXI GCAGC 3 cut(s) 74, 331, 771
BseYI CCCAGC 1 cut(s) 593
BsgI GTGCAG 1 cut(s) 150
Bsh1236I CGCG 2 cut(s) 657, 722
BshFI GGCC 4 cut(s) 12, 111, 276, 294
BshNI GGYRCC 2 cut(s) 170, 404
BsiHKAI GWGCWC 1 cut(s) 720
BsiSI CCGG 1 cut(s) 451
BslI CCNNNNNNNGG 3 cut(s) 19, 308, 530
BsnI GGCC 4 cut(s) 12, 111, 276, 294
Bsp1286I GDGCHC 2 cut(s) 409, 720
Bsp1407I TGTACA 2 cut(s) 340, 821
Bsp19I CCATGG 2 cut(s) 277, 432
BspACI CCGC 3 cut(s) 90, 112, 722
BspANI GGCC 4 cut(s) 12, 111, 276, 294
BspFNI CGCG 2 cut(s) 657, 722
BspLI GGNNCC 4 cut(s) 172, 232, 406, 455
BspQI GCTCTTC 1 cut(s) 708
BspT107I GGYRCC 2 cut(s) 170, 404
BsrGI TGTACA 2 cut(s) 340, 821
BsrI ACTGG 1 cut(s) 554
BssECI CCNNGG 2 cut(s) 277, 432
BssT1I CCWWGG 2 cut(s) 277, 432
Bst2UI CCWGG 2 cut(s) 309, 525
Bst4CI ACNGT 1 cut(s) 632
Bst6I CTCTTC 1 cut(s) 708
BstAUI TGTACA 2 cut(s) 340, 821
BstC8I GCNNGC 5 cut(s) 200, 521, 720, 782, 789
BstDSI CCRYGG 2 cut(s) 277, 432
BstF5I GGATG 1 cut(s) 263
BstFNI CGCG 2 cut(s) 657, 722
BstMWI GCNNNNNNNGC 3 cut(s) 87, 137, 179
BstNI CCWGG 2 cut(s) 309, 525
BstNSI RCATGY 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 307, 523
BstSLI GKGCMC 1 cut(s) 409
BstUI CGCG 2 cut(s) 657, 722
BstV1I GCAGC 3 cut(s) 74, 331, 771
BstV2I GAAGAC 1 cut(s) 765
BstXI CCANNNNNNTGG 1 cut(s) 284
BsuRI GGCC 4 cut(s) 12, 111, 276, 294
BtgI CCRYGG 2 cut(s) 277, 432
BtsCI GGATG 1 cut(s) 263
BtsIMutI CAGTG 3 cut(s) 140, 174, 637
Cac8I GCNNGC 5 cut(s) 200, 521, 720, 782, 789
CseI GACGC 1 cut(s) 663
Csp6I GTAC 4 cut(s) 134, 341, 749, 822
CviAII CATG 4 cut(s) 154, 203, 278, 433
CviQI GTAC 4 cut(s) 134, 341, 749, 822
DraI TTTAAA 1 cut(s) 444
EaeI YGGCCR 2 cut(s) 109, 274
Eam1104I CTCTTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 708
Ecl136II GAGCTC 1 cut(s) 718
Eco130I CCWWGG 2 cut(s) 277, 432
Eco24I GRGCYC 1 cut(s) 720
Eco53kI GAGCTC 1 cut(s) 718
Eco57I CTGAAG 1 cut(s) 638
EcoICRI GAGCTC 1 cut(s) 718
EcoRII CCWGG 2 cut(s) 307, 523
EcoT14I CCWWGG 2 cut(s) 277, 432
EcoT22I ATGCAT 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 720
ErhI CCWWGG 2 cut(s) 277, 432
FaeI CATG 4 cut(s) 157, 206, 281, 436
FaiI YATR 7 cut(s) 119, 155, 204, 279, 389, 434, 531
FatI CATG 4 cut(s) 153, 202, 277, 432
Fnu4HI GCNGC 4 cut(s) 88, 112, 320, 785
FokI GGATG 1 cut(s) 270
FriOI GRGCYC 1 cut(s) 720
Fsp4HI GCNGC 4 cut(s) 88, 112, 320, 785
FspBI CTAG 3 cut(s) 125, 752, 777
GluI GCNGC 4 cut(s) 88, 112, 320, 785
GsaI CCCAGC 1 cut(s) 597
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 4 cut(s) 12, 111, 276, 294
HapII CCGG 1 cut(s) 451
HgaI GACGC 1 cut(s) 663
Hin1II CATG 4 cut(s) 157, 206, 281, 436
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 5 cut(s) 24, 30, 37, 145, 607
HpaII CCGG 1 cut(s) 451
HphI GGTGA 1 cut(s) 765
Hpy166II GTNNAC 5 cut(s) 45, 208, 489, 635, 822
Hpy188I TCNGA 6 cut(s) 29, 104, 303, 606, 618, 667
Hpy188III TCNNGA 1 cut(s) 34
Hpy8I GTNNAC 5 cut(s) 45, 208, 489, 635, 822
HpyAV CCTTC 4 cut(s) 217, 613, 638, 689
HpyCH4III ACNGT 1 cut(s) 632
HpyCH4IV ACGT 1 cut(s) 535
HpyCH4V TGCA 5 cut(s) 131, 182, 319, 424, 787
HpyF10VI GCNNNNNNNGC 3 cut(s) 87, 137, 179
HpySE526I ACGT 1 cut(s) 535
Hsp92II CATG 4 cut(s) 157, 206, 281, 436
LguI GCTCTTC 1 cut(s) 708
LmnI GCTCC 3 cut(s) 230, 304, 453
Lsp1109I GCAGC 3 cut(s) 74, 331, 771
LweI GCATC 2 cut(s) 169, 518
MaeI CTAG 3 cut(s) 125, 752, 777
MaeII ACGT 1 cut(s) 535
MaeIII GTNAC 1 cut(s) 473
MboII GAAGA 3 cut(s) 725, 770, 855
MhlI GDGCHC 2 cut(s) 409, 720
MlsI TGGCCA 1 cut(s) 276
MluCI AATT 4 cut(s) 324, 329, 375, 801
MluI ACGCGT 1 cut(s) 655
MluNI TGGCCA 1 cut(s) 276
MmeI TCCRAC 1 cut(s) 645
MnlI CCTC 5 cut(s) 161, 213, 419, 654, 718
Mox20I TGGCCA 1 cut(s) 276
Mph1103I ATGCAT 1 cut(s) 184
MroXI GAANNNNTTC 1 cut(s) 588
MscI TGGCCA 1 cut(s) 276
MseI TTAA 2 cut(s) 443, 804
MslI CAYNNNNRTG 1 cut(s) 817
Msp20I TGGCCA 1 cut(s) 276
MspI CCGG 1 cut(s) 451
MspR9I CCNGG 2 cut(s) 309, 525
MvaI CCWGG 2 cut(s) 309, 525
MvnI CGCG 2 cut(s) 657, 722
MwoI GCNNNNNNNGC 3 cut(s) 87, 137, 179
NcoI CCATGG 2 cut(s) 277, 432
NlaIII CATG 4 cut(s) 157, 206, 281, 436
NlaIV GGNNCC 4 cut(s) 172, 232, 406, 455
NsiI ATGCAT 1 cut(s) 184
NspI RCATGY 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 708
PdmI GAANNNNTTC 1 cut(s) 588
PfeI GAWTC 5 cut(s) 24, 30, 37, 145, 607
PkrI GCNGC 4 cut(s) 89, 113, 321, 786
Psp124BI GAGCTC 1 cut(s) 720
Psp6I CCWGG 2 cut(s) 307, 523
PspFI CCCAGC 1 cut(s) 593
PspGI CCWGG 2 cut(s) 307, 523
PspN4I GGNNCC 4 cut(s) 172, 232, 406, 455
RsaI GTAC 4 cut(s) 135, 342, 750, 823
RsaNI GTAC 4 cut(s) 134, 341, 749, 822
RseI CAYNNNNRTG 1 cut(s) 817
SacI GAGCTC 1 cut(s) 720
SapI GCTCTTC 1 cut(s) 708
SaqAI TTAA 2 cut(s) 443, 804
SatI GCNGC 4 cut(s) 88, 112, 320, 785
ScaI AGTACT 1 cut(s) 750
ScrFI CCNGG 2 cut(s) 309, 525
SduI GDGCHC 2 cut(s) 409, 720
SfaNI GCATC 2 cut(s) 169, 518
SmiMI CAYNNNNRTG 1 cut(s) 817
SmlI CTYRAG 1 cut(s) 513
SmoI CTYRAG 1 cut(s) 513
SpeI ACTAGT 1 cut(s) 751
Sse9I AATT 4 cut(s) 324, 329, 375, 801
SsiI CCGC 3 cut(s) 90, 112, 722
SspMI CTAG 3 cut(s) 125, 752, 777
SstI GAGCTC 1 cut(s) 720
StyD4I CCNGG 2 cut(s) 307, 523
StyI CCWWGG 2 cut(s) 277, 432
TaaI ACNGT 1 cut(s) 632
TaiI ACGT 1 cut(s) 538
TaqI TCGA 2 cut(s) 577, 841
TasI AATT 4 cut(s) 324, 329, 375, 801
TatI WGTACW 3 cut(s) 340, 748, 821
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 5 cut(s) 24, 30, 37, 145, 607
Tru1I TTAA 2 cut(s) 443, 804
Tru9I TTAA 2 cut(s) 443, 804
TscAI CASTG 3 cut(s) 147, 181, 637
TseI GCWGC 3 cut(s) 87, 319, 784
TspDTI ATGAA 2 cut(s) 380, 657
TspGWI ACGGA 2 cut(s) 30, 807
TspRI CASTG 3 cut(s) 147, 181, 637
XapI RAATTY 2 cut(s) 324, 375
XceI RCATGY 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 588
XspI CTAG 3 cut(s) 125, 752, 777
ZrmI AGTACT 1 cut(s) 750
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.