Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003378

Physical Location & Seq

Genomic Coordinates Reverse (-)

7908257 .. 7913484

Gene Structure 5228 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1060500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2061 bp

ATGGAGGGGGTGGATCATTTGGCGCAAGAACGGAGCAAGGCGCAGTTCGATGTCGACCAGATGAAGGTCATCTGGGCCGGCTCTCGCCACACCTTGGAAGTCTCCGATCGAATTGCTCGCCTCGTCGCTAGCGATCCGGTCTTTAGAAAGGATAACAGAACATTGCTGAGTAGGAAGGATTTGTTTAAAAACACTCTGCAAAAAGCAGCTCATGCTTGGAAACGGATCACAGAGCTGCGGCTATCCGAGGAAGAGGCATACTGGTTAAGGCATTATGTGGATCAACCAGCATATACGGATCTTCACTGGGGAATGTTCGTGCCAGCTATTAAAGGATCAGGTACTGAGGAGCAGCAAAAGAAGTGGTTGCCGCTGGCATACAAAATGCAAATCATTGGTTGCTATGCTCAGACTGAACTTGGTCATGGCTCCAATGTTCAAGGGCTGGAGACCACTGCAACTTTCGATCCAAAGACTGACGAGTTTGTTATTAATAGTCCTACTCTCACATCAAGCAAATGGTGGCCTGGTGGTTTAGGAAAGGCTTCTACACATGCTGTTGTTTATGCCCGTCTTATCACTGATGGTCAAGACCATGGAGTGCATGGTTTCATTGTCCAACTTCGGAGCTTGGATGATCACTTACCACTTCCAGGCATAATAGTTGGCGATATTGGGATGAAATTTGGAAGTGGGGCATACAACTCCATGGACAATGGTGTTCTTAGACTTGATCATGTCCGTATACCAAGGGAACAAATGTTGATGAGGGTCTCACAGGTCACAAGAGAAGGAAAATACGTACAATCCAATGTTCCACGACAGCTGCTGTATGGAACTATGGTTTATGTGCGGCAAACGATTGTAGCTGATGCTTCCTCTGCTTTATCACGGGCAGTATGTATTGCTACTAGGTATAGTGCCGTTCGTCGACAATTTGGTTCACAAGATGGTGGCCTTGAAACACAGGTCATTGATTACAAGACTCAGCAAAGTAGGCTCTTCCCTTTGCTGGCTTCTGCCTATGCTTTTAGATTTGTTGGTGAGTGGTTGAAATGGCTATACACAGATGTGACAGAGAAGTTGCAAGCTAATGATTTCTCGACATTGCCCGAGGCTCATGCATGCACTGCGGGTCTGAAATCATTGACCACTTCTGCAACTGCTGATGGCATTGAAGAATGCCGGAAATTATGTGGTGGCCATGGTTATCTATCTAACAGTGGGCTTCCTGAATTATTTGCCGTTTATGTACCAGCATGTACATATGAAGGAGACAACATTGTTCTACTTCTACAGGTTGCAAGGTTTCTTATGAAGACTGTTGCTCAGCTACCATCTGGTAAAAAACCTGTTGGAACCACAACTTATATGGGCCGTGCTCAACATTTGATCCAATGTCGTTCTAGTGCTCAAAAAGGTTGTATTTTTTTTTCTATATCCAATCTATTGGCTTTCTTTACCTTTTTCACATTGTCATGCACAACGGAGGATTGGTTGAACCCTAGTGTGATACTTGAGGTATTTGAAGCAAGGGCTATAAGGATGTCGATTGCCTGTGCTCAAGACCTTAGCAAGTTTTCAAATCAAGAAGAAGGCTTTGCAGAACTCTCATCTAATTTAGCTGAGGCAGCAGTAGCTCATTGCCAATTAATTGTTGTTTCCAAGTTCATTGATAAAGTCCAACAAGACATACCGGGGAAGGGGGTGAAAAAACAGCTGGAGATCCTCTGCAACATCTATGCTTTATACACTATTCACAAGCATTTGGGTGATTTCCTCTCCACTGGAAGCCTCACAGCAAAGCAAGCTTCACTTGCAAATGATCAACTTAGATCTCTTTATTCCCAGCTCCGTCCCAATGCCATTGCCCTCGTTGATGCATTTAATTTCACCGACCACTACCTTGGCTCAATTCTAGGCCGCTATGATGGTAATGTGTACCCGAATCTCTATGAGGAGGCTTGGAAAGATCCTCTTAATGAGTCAGTTGTGCCCGATGGCTATCGGGAATATGTCCAGCCTCTGCTGAAGAACCAGCTCCGTAACGCAAGACTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001676 GO:0003674 GO:0003824 GO:0003995 GO:0003997 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010035 GO:0010038 GO:0010817 GO:0016042 GO:0016053 GO:0016054 GO:0016491 GO:0016627 GO:0016634 GO:0019395 GO:0019752 GO:0030054 GO:0030258 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0042221 GO:0042445 GO:0042446 GO:0042579 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0046686 GO:0048037 GO:0050660 GO:0050662 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072329 GO:0072330 GO:0097159 GO:1901265 GO:1901363 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01040 ko01100 ko01110 ko01212 ko03320 ko04024 ko04146 map00071 map00592 map01040 map01100 map01110 map01212 map03320 map04024 map04146

Pfam Domains

Protein Families

ACOX Acyl-CoA_dh_M Acyl-CoA_ox_N

Protein Analysis

687

Amino Acids

76.72

Weight (kDa)

7.89

Isoelectric Point (pI)

41.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-CoA_ox_N	PF14749	17 - 132	1.2e-33	Acyl-coenzyme A oxidase N-terminal
Acyl-CoA_dh_M	PF02770	134 - 233	4.3e-08	Acyl-CoA dehydrogenase, middle domain
ACOX_C_alpha1	PF22924	278 - 439	3.6e-67	Acyl-CoA oxidase, C-alpha1 domain
ACOX	PF01756	501 - 678	2.7e-54	Acyl-CoA oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003378)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35690 AT4G16760 AT4G16760
fragaria_vesca	FvH4_5g19470
malus_domestica	MD04G1060500.v1.1 MD13G1127300.v1.1
prunus_persica	Prupe.5G065100_v2.0.a1 Prupe.7G067100_v2.0.a1
pyrus_communis	pycom04g05410 pycom13g11040
rosa_chinensis	RchiOBHm_Chr3g0481201 RchiOBHm_Chr3g0481221 RchiOBHm_Chr7g0204041
rosa_laevigata	RLG00000003492 RLG00000023411 RLG00000023414 RLG00000023415
rosa_multiflora	Rmu_sc0000064.1_g000003 Rmu_sc0001311.1_g000009 Rmu_sc0001551.1_g000009 Rmu_sc0001843.1_g000010 Rmu_sc0009910.1_g000005
rosa_roxburghii	Rroxscaffold_3G00253400 Rroxscaffold_6G00400590
rosa_rugosa	Rorug03G0190400 Rorug07G0080100 Rorug07G0080200
rosa_samantha	Rh3AG241300 Rh3BG276600 Rh3DG268000 Rh7AG210200 Rh7BG208600
rosa_wichuraiana	Rw3G021520 Rw3G021530 Rw3G021550 Rw7G018300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	94
AccI	GTMKAC	3 cut(s)	54, 745, 931
AciI	CCGC	5 cut(s)	238, 371, 853, 1133, 1924
AcoI	YGGCCR	1 cut(s)	1201
AcsI	RAATTY	1 cut(s)	683
AcuI	CTGAAG	1 cut(s)	2051
AfaI	GTAC	5 cut(s)	343, 804, 1254, 1264, 1943
AfiI	CCNNNNNNNGG	5 cut(s)	64, 94, 653, 1012, 1703
AgsI	TTSAA	7 cut(s)	440, 962, 1054, 1178, 1501, 1529, 1584
AjnI	CCWGG	2 cut(s)	526, 652
AleI	CACNNNNGTG	1 cut(s)	1070
Alw21I	GWGCWC	3 cut(s)	1384, 1414, 1564
Alw26I	GTCTC	4 cut(s)	106, 443, 778, 1269
AlwNI	CAGNNNCTG	3 cut(s)	344, 829, 2026
Ama87I	CYCGRG	1 cut(s)	1112
AoxI	GGCC	6 cut(s)	75, 524, 955, 1201, 1375, 1921
ApeKI	GCWGC	5 cut(s)	206, 235, 352, 826, 1631
ApoI	RAATTY	1 cut(s)	683
AseI	ATTAAT	2 cut(s)	492, 1652
Asp700I	GAANNNNTTC	1 cut(s)	544
AspLEI	GCGC	2 cut(s)	25, 43
AspS9I	GGNCC	2 cut(s)	75, 1375
AsuC2I	CCSGG	1 cut(s)	1698
AsuHPI	GGTGA	4 cut(s)	1055, 1720, 1784, 1885
AsuNHI	GCTAGC	1 cut(s)	128
AvaI	CYCGRG	1 cut(s)	1112
BaeGI	GKGCMC	1 cut(s)	1998
BalI	TGGCCA	1 cut(s)	1203
BbsI	GAAGAC	1 cut(s)	1325
Bbv12I	GWGCWC	3 cut(s)	1384, 1414, 1564
BbvCI	CCTCAGC	1 cut(s)	1626
BbvI	GCAGC	5 cut(s)	218, 222, 364, 813, 1643
BccI	CCATC	6 cut(s)	578, 944, 1163, 1345, 1925, 1994
BceAI	ACGGC	3 cut(s)	908, 1229, 1362
BcgI	CGANNNNNNTGC	4 cut(s)	95, 129, 1103, 1137
BciT130I	CCWGG	2 cut(s)	528, 654
BclI	TGATCA	3 cut(s)	637, 733, 1825
BcnI	CCSGG	1 cut(s)	1698
BcoDI	GTCTC	4 cut(s)	106, 443, 778, 1269
BfaI	CTAG	5 cut(s)	129, 912, 1407, 1506, 1919
BfmI	CTRYAG	1 cut(s)	1295
BglII	AGATCT	1 cut(s)	1835
BisI	GCNGC	9 cut(s)	207, 236, 239, 353, 371, 827, 854, 1632, 1924
BlpI	GCTNAGC	1 cut(s)	1329
BlsI	GCNGC	9 cut(s)	208, 237, 240, 354, 372, 828, 855, 1633, 1925
Bme1390I	CCNGG	3 cut(s)	528, 654, 1698
BmeT110I	CYCGRG	1 cut(s)	1112
BmgT120I	GGNCC	2 cut(s)	75, 1375
BmiI	GGNNCC	2 cut(s)	430, 1360
BmrFI	CCNGG	3 cut(s)	528, 654, 1698
BmrI	ACTGGG	1 cut(s)	316
BmsI	GCATC	2 cut(s)	862, 1870
BmtI	GCTAGC	1 cut(s)	132
BmuI	ACTGGG	1 cut(s)	316
BpiI	GAAGAC	1 cut(s)	1325
BpmI	CTGGAG	2 cut(s)	467, 1742
Bpu10I	CCTNAGC	2 cut(s)	1571, 1626
Bpu1102I	GCTNAGC	1 cut(s)	1329
BpuEI	CTTGAG	2 cut(s)	1538, 1548
BpuMI	CCSGG	1 cut(s)	1698
BsaAI	YACGTR	1 cut(s)	802
BsaBI	GATNNNNATC	1 cut(s)	2004
BsaI	GGTCTC	2 cut(s)	443, 778
BsaJI	CCNNGG	9 cut(s)	93, 246, 595, 708, 749, 1113, 1204, 1697, 1906
BsaWI	WCCGGW	1 cut(s)	136
BsaXI	ACNNNNNCTCC	2 cut(s)	1481, 1511
Bsc4I	CCNNNNNNNGG	5 cut(s)	64, 94, 653, 1012, 1703
Bse118I	RCCGGY	1 cut(s)	77
Bse1I	ACTGG	3 cut(s)	266, 311, 1792
Bse3DI	GCAATG	4 cut(s)	161, 1106, 1642, 1866
Bse8I	GATNNNNATC	1 cut(s)	2004
BseBI	CCWGG	2 cut(s)	528, 654
BseDI	CCNNGG	9 cut(s)	93, 246, 595, 708, 749, 1113, 1204, 1697, 1906
BseGI	GGATG	3 cut(s)	640, 684, 1551
BseJI	GATNNNNATC	1 cut(s)	2004
BseLI	CCNNNNNNNGG	5 cut(s)	64, 94, 653, 1012, 1703
BseMI	GCAATG	4 cut(s)	161, 1106, 1642, 1866
BseMII	CTCAG	6 cut(s)	158, 336, 422, 1001, 1343, 1617
BseNI	ACTGG	3 cut(s)	266, 311, 1792
BseRI	GAGGAG	2 cut(s)	362, 1973
BseSI	GKGCMC	1 cut(s)	1998
BseXI	GCAGC	5 cut(s)	218, 222, 364, 813, 1643
BseYI	CCCAGC	1 cut(s)	1848
Bsh1285I	CGRYCG	1 cut(s)	109
BshFI	GGCC	6 cut(s)	77, 526, 957, 1203, 1377, 1923
BsiEI	CGRYCG	1 cut(s)	109
BsiHKAI	GWGCWC	3 cut(s)	1384, 1414, 1564
BsiHKCI	CYCGRG	1 cut(s)	1112
BsiSI	CCGG	4 cut(s)	78, 137, 1186, 1697
BslFI	GGGAC	1 cut(s)	1842
BslI	CCNNNNNNNGG	5 cut(s)	64, 94, 653, 1012, 1703
BsmAI	GTCTC	4 cut(s)	106, 443, 778, 1269
BsmFI	GGGAC	1 cut(s)	1842
BsmI	GAATGC	1 cut(s)	1187
BsnI	GGCC	6 cut(s)	77, 526, 957, 1203, 1377, 1923
Bso31I	GGTCTC	2 cut(s)	443, 778
BsoBI	CYCGRG	1 cut(s)	1112
Bsp1286I	GDGCHC	4 cut(s)	1384, 1414, 1564, 1998
Bsp1407I	TGTACA	1 cut(s)	1262
Bsp1720I	GCTNAGC	1 cut(s)	1329
Bsp19I	CCATGG	3 cut(s)	595, 708, 1204
BspACI	CCGC	5 cut(s)	238, 371, 853, 1133, 1924
BspANI	GGCC	6 cut(s)	77, 526, 957, 1203, 1377, 1923
BspCNI	CTCAG	6 cut(s)	159, 337, 421, 1000, 1342, 1618
BspLI	GGNNCC	2 cut(s)	430, 1360
BspOI	GCTAGC	1 cut(s)	132
BspQI	GCTCTTC	1 cut(s)	1007
BspTNI	GGTCTC	2 cut(s)	443, 778
BsrDI	GCAATG	4 cut(s)	161, 1106, 1642, 1866
BsrFI	RCCGGY	1 cut(s)	77
BsrGI	TGTACA	1 cut(s)	1262
BsrI	ACTGG	3 cut(s)	266, 311, 1792
BssAI	RCCGGY	1 cut(s)	77
BssECI	CCNNGG	9 cut(s)	93, 246, 595, 708, 749, 1113, 1204, 1697, 1906
BssNAI	GTATAC	1 cut(s)	746
BssT1I	CCWWGG	6 cut(s)	93, 595, 708, 749, 1204, 1906
Bst1107I	GTATAC	1 cut(s)	746
Bst2UI	CCWGG	2 cut(s)	528, 654
Bst4CI	ACNGT	2 cut(s)	1223, 1324
Bst6I	CTCTTC	2 cut(s)	246, 1007
BstAPI	GCANNNNNTGC	2 cut(s)	212, 1130
BstAUI	TGTACA	1 cut(s)	1262
BstBAI	YACGTR	1 cut(s)	802
BstC8I	GCNNGC	9 cut(s)	79, 118, 130, 324, 375, 1014, 1089, 1126, 1809
BstDEI	CTNAG	9 cut(s)	167, 345, 408, 725, 987, 1329, 1571, 1626, 1832
BstDSI	CCRYGG	3 cut(s)	595, 708, 1204
BstF5I	GGATG	3 cut(s)	640, 684, 1551
BstHHI	GCGC	2 cut(s)	25, 43
BstMAI	GTCTC	4 cut(s)	106, 443, 778, 1269
BstMCI	CGRYCG	1 cut(s)	109
BstMWI	GCNNNNNNNGC	8 cut(s)	212, 881, 997, 1130, 1631, 1637, 1808, 1817
BstNI	CCWGG	2 cut(s)	528, 654
BstNSI	RCATGY	3 cut(s)	557, 1128, 1263
BstSCI	CCNGG	3 cut(s)	526, 652, 1696
BstSFI	CTRYAG	1 cut(s)	1295
BstSLI	GKGCMC	1 cut(s)	1998
BstSNI	TACGTA	1 cut(s)	802
BstV1I	GCAGC	5 cut(s)	218, 222, 364, 813, 1643
BstV2I	GAAGAC	1 cut(s)	1325
BstX2I	RGATCY	4 cut(s)	298, 1725, 1835, 1972
BstXI	CCANNNNNNTGG	2 cut(s)	1450, 1907
BstYI	RGATCY	4 cut(s)	298, 1725, 1835, 1972
BstZ17I	GTATAC	1 cut(s)	746
BsuRI	GGCC	6 cut(s)	77, 526, 957, 1203, 1377, 1923
BtgI	CCRYGG	3 cut(s)	595, 708, 1204
BtsCI	GGATG	3 cut(s)	640, 684, 1551
BtsI	GCAGTG	2 cut(s)	453, 1128
BtsIMutI	CAGTG	6 cut(s)	304, 453, 579, 1128, 1228, 1785
Cac8I	GCNNGC	9 cut(s)	79, 118, 130, 324, 375, 1014, 1089, 1126, 1809
CaiI	CAGNNNCTG	3 cut(s)	344, 829, 2026
CfoI	GCGC	2 cut(s)	25, 43
Cfr10I	RCCGGY	1 cut(s)	77
Cfr13I	GGNCC	2 cut(s)	75, 1375
Csp6I	GTAC	5 cut(s)	342, 803, 1253, 1263, 1942
CspCI	CAANNNNNGTGG	2 cut(s)	344, 379
CviQI	GTAC	5 cut(s)	342, 803, 1253, 1263, 1942
DdeI	CTNAG	9 cut(s)	167, 345, 408, 725, 987, 1329, 1571, 1626, 1832
DraI	TTTAAA	1 cut(s)	187
EaeI	YGGCCR	1 cut(s)	1201
Eam1104I	CTCTTC	2 cut(s)	246, 1007
EarI	CTCTTC	2 cut(s)	246, 1007
Eco105I	TACGTA	1 cut(s)	802
Eco130I	CCWWGG	6 cut(s)	93, 595, 708, 749, 1204, 1906
Eco31I	GGTCTC	2 cut(s)	443, 778
Eco57I	CTGAAG	1 cut(s)	2051
Eco88I	CYCGRG	1 cut(s)	1112
EcoRII	CCWGG	2 cut(s)	526, 652
EcoT14I	CCWWGG	6 cut(s)	93, 595, 708, 749, 1204, 1906
EcoT22I	ATGCAT	2 cut(s)	1126, 1885
ErhI	CCWWGG	6 cut(s)	93, 595, 708, 749, 1204, 1906
FalI	AAGNNNNNCTT	4 cut(s)	1800, 1832, 1962, 1994
FaqI	GGGAC	1 cut(s)	1842
FauI	CCCGC	1 cut(s)	1126
FauNDI	CATATG	1 cut(s)	1267
FbaI	TGATCA	3 cut(s)	637, 733, 1825
FblI	GTMKAC	3 cut(s)	54, 745, 931
Fnu4HI	GCNGC	9 cut(s)	207, 236, 239, 353, 371, 827, 854, 1632, 1924
FokI	GGATG	3 cut(s)	647, 691, 1558
Fsp4HI	GCNGC	9 cut(s)	207, 236, 239, 353, 371, 827, 854, 1632, 1924
FspBI	CTAG	5 cut(s)	129, 912, 1407, 1506, 1919
GlaI	GCGC	2 cut(s)	24, 42
GluI	GCNGC	9 cut(s)	207, 236, 239, 353, 371, 827, 854, 1632, 1924
GsaI	CCCAGC	1 cut(s)	1852
GsuI	CTGGAG	2 cut(s)	467, 1742
HaeIII	GGCC	6 cut(s)	77, 526, 957, 1203, 1377, 1923
HapII	CCGG	4 cut(s)	78, 137, 1186, 1697
HhaI	GCGC	2 cut(s)	25, 43
Hin6I	GCGC	2 cut(s)	23, 41
HinP1I	GCGC	2 cut(s)	23, 41
HincII	GTYRAC	2 cut(s)	55, 932
HindII	GTYRAC	2 cut(s)	55, 932
HindIII	AAGCTT	1 cut(s)	1809
HinfI	GANTC	3 cut(s)	985, 1948, 1985
HpaII	CCGG	4 cut(s)	78, 137, 1186, 1697
HphI	GGTGA	4 cut(s)	1055, 1720, 1784, 1885
Hpy166II	GTNNAC	5 cut(s)	55, 746, 932, 944, 1942
Hpy188I	TCNGA	5 cut(s)	106, 247, 411, 627, 1140
Hpy188III	TCNNGA	6 cut(s)	590, 1102, 1232, 1565, 1589, 2009
Hpy8I	GTNNAC	5 cut(s)	55, 746, 932, 944, 1942
Hpy99I	CGWCG	2 cut(s)	128, 933
HpyAV	CCTTC	6 cut(s)	58, 169, 785, 1265, 1589, 1696
HpyCH4III	ACNGT	2 cut(s)	1223, 1324
HpyCH4IV	ACGT	1 cut(s)	801
HpyF10VI	GCNNNNNNNGC	8 cut(s)	212, 881, 997, 1130, 1631, 1637, 1808, 1817
HpyF3I	CTNAG	9 cut(s)	167, 345, 408, 725, 987, 1329, 1571, 1626, 1832
HpySE526I	ACGT	1 cut(s)	801
HspAI	GCGC	2 cut(s)	23, 41
KroI	GCCGGC	1 cut(s)	77
KroNI	GCCGGC	1 cut(s)	79
Ksp22I	TGATCA	3 cut(s)	637, 733, 1825
LguI	GCTCTTC	1 cut(s)	1007
LmnI	GCTCC	6 cut(s)	33, 349, 434, 627, 1857, 2046
Lsp1109I	GCAGC	5 cut(s)	218, 222, 364, 813, 1643
LweI	GCATC	2 cut(s)	862, 1870
MaeI	CTAG	5 cut(s)	129, 912, 1407, 1506, 1919
MaeII	ACGT	1 cut(s)	801
MaeIII	GTNAC	3 cut(s)	781, 1072, 2045
MboII	GAAGA	7 cut(s)	263, 293, 994, 1190, 1330, 1604, 2044
MflI	RGATCY	4 cut(s)	298, 1725, 1835, 1972
MhlI	GDGCHC	4 cut(s)	1384, 1414, 1564, 1998
MlsI	TGGCCA	1 cut(s)	1203
MluNI	TGGCCA	1 cut(s)	1203
MlyI	GAGTC	2 cut(s)	979, 1994
MmeI	TCCRAC	3 cut(s)	643, 1336, 1708
Mox20I	TGGCCA	1 cut(s)	1203
Mph1103I	ATGCAT	2 cut(s)	1126, 1885
MroNI	GCCGGC	1 cut(s)	77
MroXI	GAANNNNTTC	1 cut(s)	544
MscI	TGGCCA	1 cut(s)	1203
MseI	TTAA	7 cut(s)	186, 266, 330, 492, 1652, 1887, 1980
MslI	CAYNNNNRTG	3 cut(s)	1070, 1477, 1770
Msp20I	TGGCCA	1 cut(s)	1203
MspA1I	CMGCKG	3 cut(s)	373, 826, 1720
MspI	CCGG	4 cut(s)	78, 137, 1186, 1697
MspR9I	CCNGG	3 cut(s)	528, 654, 1698
Mva1269I	GAATGC	1 cut(s)	1187
MvaI	CCWGG	2 cut(s)	528, 654
MwoI	GCNNNNNNNGC	8 cut(s)	212, 881, 997, 1130, 1631, 1637, 1808, 1817
NaeI	GCCGGC	1 cut(s)	79
NciI	CCSGG	1 cut(s)	1698
NcoI	CCATGG	3 cut(s)	595, 708, 1204
NdeI	CATATG	1 cut(s)	1267
NgoMIV	GCCGGC	1 cut(s)	77
NheI	GCTAGC	1 cut(s)	128
NlaIV	GGNNCC	2 cut(s)	430, 1360
NmuCI	GTSAC	2 cut(s)	781, 1072
NsiI	ATGCAT	2 cut(s)	1126, 1885
NspI	RCATGY	3 cut(s)	557, 1128, 1263
OliI	CACNNNNGTG	1 cut(s)	1070
PaeI	GCATGC	1 cut(s)	1128
PciSI	GCTCTTC	1 cut(s)	1007
PcsI	WCGNNNNNNNCGW	1 cut(s)	129
PctI	GAATGC	1 cut(s)	1187
PdiI	GCCGGC	1 cut(s)	79
PdmI	GAANNNNTTC	1 cut(s)	544
PfeI	GAWTC	1 cut(s)	1948
PflMI	CCANNNNNTGG	1 cut(s)	94
PkrI	GCNGC	9 cut(s)	208, 237, 240, 354, 372, 828, 855, 1633, 1925
Ple19I	CGATCG	1 cut(s)	109
PleI	GAGTC	2 cut(s)	979, 1993
PpsI	GAGTC	2 cut(s)	979, 1993
Ppu21I	YACGTR	1 cut(s)	802
PshBI	ATTAAT	2 cut(s)	492, 1652
Psp6I	CCWGG	2 cut(s)	526, 652
PspFI	CCCAGC	1 cut(s)	1848
PspGI	CCWGG	2 cut(s)	526, 652
PspN4I	GGNNCC	2 cut(s)	430, 1360
PspPI	GGNCC	2 cut(s)	75, 1375
PstNI	CAGNNNCTG	3 cut(s)	344, 829, 2026
PsuI	RGATCY	4 cut(s)	298, 1725, 1835, 1972
PvuI	CGATCG	1 cut(s)	109
PvuII	CAGCTG	2 cut(s)	826, 1720
RsaI	GTAC	5 cut(s)	343, 804, 1254, 1264, 1943
RsaNI	GTAC	5 cut(s)	342, 803, 1253, 1263, 1942
RseI	CAYNNNNRTG	3 cut(s)	1070, 1477, 1770
SalI	GTCGAC	2 cut(s)	53, 930
SapI	GCTCTTC	1 cut(s)	1007
SaqAI	TTAA	7 cut(s)	186, 266, 330, 492, 1652, 1887, 1980
SatI	GCNGC	9 cut(s)	207, 236, 239, 353, 371, 827, 854, 1632, 1924
Sau96I	GGNCC	2 cut(s)	75, 1375
SchI	GAGTC	2 cut(s)	979, 1994
ScrFI	CCNGG	3 cut(s)	528, 654, 1698
SduI	GDGCHC	4 cut(s)	1384, 1414, 1564, 1998
SfaNI	GCATC	2 cut(s)	862, 1870
SfcI	CTRYAG	1 cut(s)	1295
SmiMI	CAYNNNNRTG	3 cut(s)	1070, 1477, 1770
SmlI	CTYRAG	2 cut(s)	1517, 1563
SmoI	CTYRAG	2 cut(s)	1517, 1563
SnaBI	TACGTA	1 cut(s)	802
SphI	GCATGC	1 cut(s)	1128
SsiI	CCGC	5 cut(s)	238, 371, 853, 1133, 1924
SspMI	CTAG	5 cut(s)	129, 912, 1407, 1506, 1919
StyD4I	CCNGG	3 cut(s)	526, 652, 1696
StyI	CCWWGG	6 cut(s)	93, 595, 708, 749, 1204, 1906
TaaI	ACNGT	2 cut(s)	1223, 1324
TaiI	ACGT	1 cut(s)	804
TaqI	TCGA	7 cut(s)	48, 54, 109, 465, 931, 1103, 1550
TatI	WGTACW	1 cut(s)	1262
TauI	GCSGC	4 cut(s)	241, 373, 856, 1926
TfiI	GAWTC	1 cut(s)	1948
Tru1I	TTAA	7 cut(s)	186, 266, 330, 492, 1652, 1887, 1980
Tru9I	TTAA	7 cut(s)	186, 266, 330, 492, 1652, 1887, 1980
TscAI	CASTG	6 cut(s)	311, 460, 586, 1135, 1228, 1792
TseFI	GTSAC	2 cut(s)	781, 1072
TseI	GCWGC	5 cut(s)	206, 235, 352, 826, 1631
Tsp45I	GTSAC	2 cut(s)	781, 1072
TspDTI	ATGAA	6 cut(s)	77, 601, 695, 1284, 1331, 1660
TspGWI	ACGGA	7 cut(s)	46, 238, 311, 731, 1502, 1844, 2033
TspRI	CASTG	6 cut(s)	311, 460, 586, 1135, 1228, 1792
Van91I	CCANNNNNTGG	1 cut(s)	94
VspI	ATTAAT	2 cut(s)	492, 1652
XapI	RAATTY	1 cut(s)	683
XceI	RCATGY	3 cut(s)	557, 1128, 1263
XcmI	CCANNNNNNNNNTGG	2 cut(s)	602, 1369
XmiI	GTMKAC	3 cut(s)	54, 745, 931
XmnI	GAANNNNTTC	1 cut(s)	544
XspI	CTAG	5 cut(s)	129, 912, 1407, 1506, 1919
Zsp2I	ATGCAT	2 cut(s)	1126, 1885

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.