Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003378

Physical Location & Seq

Genomic Coordinates Reverse (-)

9572286 .. 9574996

Gene Structure 2711 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1127300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGGCTTCGAGTTCTGCAACTGGTGCAGATCATTTGGCGCATGAGAGGAGCAAGGCTAAGTTTGACGTGGAGGCAATGAAGATCGTCTGGGCGGGTTCTTCCCACGAGTTTCGAGTCTCTGATCGAATGGCTCACCTCGTGGCTAGTGATCCGAGCTTTCGACGGGAGAATAGAGCTGTGCTAAGTAGGAAGGAGTTGTTCACAAAGGCTCTGAGAAAAGCAGCTCATGCCTGGAAAAGGATCAATGAGCTTGGGCTTTCAGATGAAGAGGCTTCATGGCTATGGTTTTATGTGGACGAACTGACATTCGCTTACATTCATTGGGGAATGTTTGTGCCCGCTATCAAAGGGTCAGGCACGGAGGAGCAGCAAAACAAGTGGTTGCCGTTGGCAACCACGATGCGCATAATTGGTACTTATGCACAAACTGAGCTCGGACATGGCTCGAATGTTCGAGGGATTGAGACCACCGCGACATTTGATCCAACGACTGACGAGTTCATCATCCATAGTCCTACACTCACCTCGAGCAAATGGTGGTCTGGCGGATTGGGGAAGATTGCAACTCACGCTGTTGTCTATGCCCGGGTTATTACTCACGACGGCCAAGATCATGGCGTCAATGGCTTCATTGTCCAAATTCGGAGTTTGGATGATCACTTGCCTCTTCCGGGCATAACAGTTGGTGATATAGGAACAAAATTTGGAAGCGGAGCTTTCAATTCCGTGGACAATGGTGTTCTAAGATTTGATCACGTGCGAATACCAAGAAATCAAATGCTGATGAGGGTGGCACATGTCACAAGAGAAGGAAAATATGTGCTGTCCAATGTTCCTAGGCAGCTGCTTTATGGAACTATGGTTTATGTGAGGAAAACTATTGTAGTTGATTCTTCAATTGCTTTATCAAGAGCAGTGTGTATAGCTGCTCGATATAGCAGTGTTCGTCGACAATTTGGTTCACAAAATCGTGAGATTAATGGTATTGCGGTTGAGACCCCAGTGATTGAGTACAAGACTCAGCAAAGTAGACTTTTTTCTCTGTTAGCTTCTGCCTATGCTTTCAGATTCATTGGTGAGTGGCTGAAAAGCCTATATACCGATTTGACACGAAAACTGGAAGCGAATGATTTTTCAACATTAGCTGAAGCTCATGCATGCACGGCGGGTTTGAAGTCCTTGACTACATCTTCAGCTGTTCATGGGATTGACGAATGCCGGAAACTATGTGGTGGCCATGGATACCTAAATAGCAGCGGGCTTCCCGAGTTATATGCTGTTTTCGCACCTGCATGTACGTATGAAGGGGACAACACTGTTCTTCTTTTGCAGGTTGCAAGGTTCCTCATGAAGACTATTTCTCAGCTTCTTCATTCTGATCAAACTCATAAGCTTTCTGGAACAATAGCTTATATGGTTCTGATGGATCATTATTTGATCAAATATTGCTGTAGTGTTGAAAAAGCTGAGGACTGGTTGAAAGCCGATGTAATACTCAAGGTATTCGAAGCAAGGGCCATTAGAATGTGTGTTGCTTGTGCTCAAAATCTTGCCAAGTTTGCAGATCCATCAGAAGGGTTTTCTGAACTCTCACCAGATTTAGTAGAGGCTGCAATTGCTCACTGCCAGTTGATCGTTGTCTCCAAATTCATTGAGAAATTGCAGCAGAAAATTGAAGGAAACGGAGTAAAACAACAATTGGAGACCCTCTGCTACATATATGCTTTGCATCTGATTCACAAATATTCAGGGGATTTTATTTCCACGGGTAGCTTGAATCCCAAGCAAGCTTCACTTGCCAATGATCAACTTAGAGCTCTGTATGTCGAGGTTCGTCCAAATGTTATTGCCCTTGTTGATGCATTTAATTACACTGACCATTTTCTTGGCTCAATTCTTGGATGCTACGATGGGAATGTGTATCCAAAACTGTATGATGAGGCTTGGAAAGATCCGCTCAATGACTCAGTTGTAACTGATGGCTATCCTGAATACATCAAACCTCTGATAAATCAGCAGCTCCTAAATGCAAGGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001676 GO:0003674 GO:0003824 GO:0003995 GO:0003997 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010035 GO:0010038 GO:0010817 GO:0016042 GO:0016053 GO:0016054 GO:0016491 GO:0016627 GO:0016634 GO:0019395 GO:0019752 GO:0030054 GO:0030258 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0042221 GO:0042445 GO:0042446 GO:0042579 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0046686 GO:0048037 GO:0050660 GO:0050662 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072329 GO:0072330 GO:0097159 GO:1901265 GO:1901363 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01040 ko01100 ko01110 ko01212 ko03320 ko04024 ko04146 map00071 map00592 map01040 map01100 map01110 map01212 map03320 map04024 map04146

Pfam Domains

Protein Families

ACOX Acyl-CoA_dh_M Acyl-CoA_ox_N

Protein Analysis

680

Amino Acids

75.7

Weight (kDa)

7.1

Isoelectric Point (pI)

34.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-CoA_ox_N	PF14749	22 - 137	4.2e-29	Acyl-coenzyme A oxidase N-terminal
Acyl-CoA_dh_M	PF02770	140 - 240	1.5e-09	Acyl-CoA dehydrogenase, middle domain
ACOX_C_alpha1	PF22924	284 - 450	4.1e-62	Acyl-CoA oxidase, C-alpha1 domain
ACOX	PF01756	496 - 671	3.4e-52	Acyl-CoA oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003378)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35690 AT4G16760 AT4G16760
fragaria_vesca	FvH4_5g19470
malus_domestica	MD04G1060500.v1.1 MD13G1127300.v1.1
prunus_persica	Prupe.5G065100_v2.0.a1 Prupe.7G067100_v2.0.a1
pyrus_communis	pycom04g05410 pycom13g11040
rosa_chinensis	RchiOBHm_Chr3g0481201 RchiOBHm_Chr3g0481221 RchiOBHm_Chr7g0204041
rosa_laevigata	RLG00000003492 RLG00000023411 RLG00000023414 RLG00000023415
rosa_multiflora	Rmu_sc0000064.1_g000003 Rmu_sc0001311.1_g000009 Rmu_sc0001551.1_g000009 Rmu_sc0001843.1_g000010 Rmu_sc0009910.1_g000005
rosa_roxburghii	Rroxscaffold_3G00253400 Rroxscaffold_6G00400590
rosa_rugosa	Rorug03G0190400 Rorug07G0080100 Rorug07G0080200
rosa_samantha	Rh3AG241300 Rh3BG276600 Rh3DG268000 Rh7AG210200 Rh7BG208600
rosa_wichuraiana	Rw3G021520 Rw3G021530 Rw3G021550 Rw7G018300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1297
Acc16I	TGCGCA	1 cut(s)	404
Acc36I	ACCTGC	2 cut(s)	1297, 1321
AccBSI	CCGCTC	1 cut(s)	1957
AccI	GTMKAC	2 cut(s)	949, 1030
AccII	CGCG	1 cut(s)	473
AciI	CCGC	9 cut(s)	92, 339, 471, 546, 711, 989, 1166, 1257, 1955
AclWI	GGATC	6 cut(s)	143, 248, 476, 1434, 1559, 1946
AcoI	YGGCCR	2 cut(s)	604, 1234
AcsI	RAATTY	3 cut(s)	639, 701, 1646
AcuI	CTGAAG	2 cut(s)	1167, 1176
AcvI	CACGTG	1 cut(s)	757
AcyI	GRCGYC	1 cut(s)	618
AdeI	CACNNNGTG	1 cut(s)	139
AfaI	GTAC	3 cut(s)	415, 1013, 1297
AfiI	CCNNNNNNNGG	3 cut(s)	237, 671, 1574
AflIII	ACRYGT	1 cut(s)	796
AgsI	TTSAA	8 cut(s)	721, 897, 1137, 1174, 1460, 1480, 1676, 1777
AjiI	CACGTC	1 cut(s)	67
AjnI	CCWGG	1 cut(s)	230
Alw21I	GWGCWC	3 cut(s)	435, 1543, 1819
Alw26I	GTCTC	5 cut(s)	121, 458, 989, 1645, 1697
AlwI	GGATC	6 cut(s)	143, 248, 476, 1434, 1559, 1946
Ama87I	CYCGRG	3 cut(s)	526, 585, 1265
AoxI	GGCC	3 cut(s)	604, 1234, 1515
ApeKI	GCWGC	9 cut(s)	221, 367, 841, 844, 926, 1254, 1610, 1663, 2017
ApoI	RAATTY	3 cut(s)	639, 701, 1646
AseI	ATTAAT	1 cut(s)	978
AspA2I	CCTAGG	1 cut(s)	836
AspLEI	GCGC	2 cut(s)	40, 405
AspS9I	GGNCC	1 cut(s)	1515
AsuC2I	CCSGG	3 cut(s)	586, 587, 672
AsuHPI	GGTGA	5 cut(s)	125, 514, 698, 1088, 1584
AsuII	TTCGAA	1 cut(s)	1506
AvaI	CYCGRG	3 cut(s)	526, 585, 1265
AvrII	CCTAGG	1 cut(s)	836
BaeGI	GKGCMC	1 cut(s)	339
BalI	TGGCCA	1 cut(s)	1236
BanII	GRGCYC	2 cut(s)	435, 1819
BauI	CACGAG	2 cut(s)	104, 137
BbrPI	CACGTG	1 cut(s)	757
BbsI	GAAGAC	1 cut(s)	1358
Bbv12I	GWGCWC	3 cut(s)	435, 1543, 1819
BbvCI	CCTCAGC	1 cut(s)	1467
BbvI	GCAGC	9 cut(s)	233, 379, 831, 853, 913, 1266, 1597, 1675, 2029
BccI	CCATC	4 cut(s)	1417, 1576, 1904, 1973
BceAI	ACGGC	3 cut(s)	370, 619, 1179
BcgI	CGANNNNNNTGC	2 cut(s)	1256, 1290
BciT130I	CCWGG	1 cut(s)	232
BciVI	GTATCC	2 cut(s)	1235, 1932
BclI	TGATCA	5 cut(s)	655, 751, 1378, 1437, 1804
BcnI	CCSGG	3 cut(s)	586, 587, 672
BcoDI	GTCTC	5 cut(s)	121, 458, 989, 1645, 1697
BfaI	CTAG	2 cut(s)	144, 837
BfmI	CTRYAG	1 cut(s)	1450
BfuAI	ACCTGC	2 cut(s)	1297, 1321
BfuI	GTATCC	2 cut(s)	1235, 1932
BisI	GCNGC	9 cut(s)	222, 368, 842, 845, 927, 1255, 1611, 1664, 2018
BlnI	CCTAGG	1 cut(s)	836
BlsI	GCNGC	9 cut(s)	223, 369, 843, 846, 928, 1256, 1612, 1665, 2019
Bme1390I	CCNGG	4 cut(s)	232, 586, 587, 672
BmeT110I	CYCGRG	3 cut(s)	526, 585, 1265
BmgBI	CACGTC	1 cut(s)	67
BmgT120I	GGNCC	1 cut(s)	1515
BmiI	GGNNCC	1 cut(s)	1343
BmrFI	CCNGG	4 cut(s)	232, 586, 587, 672
BmrI	ACTGGG	1 cut(s)	995
BmsI	GCATC	4 cut(s)	390, 1738, 1849, 1892
BmuI	ACTGGG	1 cut(s)	995
BpiI	GAAGAC	1 cut(s)	1358
Bpu10I	CCTNAGC	1 cut(s)	1467
Bpu14I	TTCGAA	1 cut(s)	1506
BpuEI	CTTGAG	1 cut(s)	1481
BpuMI	CCSGG	3 cut(s)	586, 587, 672
BsaAI	YACGTR	2 cut(s)	757, 1299
BsaBI	GATNNNNATC	1 cut(s)	1983
BsaHI	GRCGYC	1 cut(s)	618
BsaI	GGTCTC	3 cut(s)	458, 989, 1697
BsaJI	CCNNGG	5 cut(s)	585, 726, 836, 1237, 1764
Bsc4I	CCNNNNNNNGG	3 cut(s)	237, 671, 1574
Bse1I	ACTGG	5 cut(s)	25, 1001, 1122, 1478, 1627
Bse3DI	GCAATG	1 cut(s)	81
Bse8I	GATNNNNATC	1 cut(s)	1983
BseBI	CCWGG	1 cut(s)	232
BseDI	CCNNGG	5 cut(s)	585, 726, 836, 1237, 1764
BseGI	GGATG	3 cut(s)	504, 658, 1907
BseJI	GATNNNNATC	1 cut(s)	1983
BseLI	CCNNNNNNNGG	3 cut(s)	237, 671, 1574
BseMI	GCAATG	1 cut(s)	81
BseMII	CTCAG	6 cut(s)	203, 420, 1034, 1376, 1458, 1980
BseNI	ACTGG	5 cut(s)	25, 1001, 1122, 1478, 1627
BseRI	GAGGAG	2 cut(s)	61, 377
BseSI	GKGCMC	1 cut(s)	339
BseXI	GCAGC	9 cut(s)	233, 379, 831, 853, 913, 1266, 1597, 1675, 2029
BsgI	GTGCAG	1 cut(s)	45
Bsh1236I	CGCG	1 cut(s)	473
BshFI	GGCC	3 cut(s)	606, 1236, 1517
BsiHKAI	GWGCWC	3 cut(s)	435, 1543, 1819
BsiHKCI	CYCGRG	3 cut(s)	526, 585, 1265
BsiSI	CCGG	3 cut(s)	586, 671, 1219
BslFI	GGGAC	1 cut(s)	1322
BslI	CCNNNNNNNGG	3 cut(s)	237, 671, 1574
BsmAI	GTCTC	5 cut(s)	121, 458, 989, 1645, 1697
BsmFI	GGGAC	1 cut(s)	1322
BsmI	GAATGC	1 cut(s)	1220
BsnI	GGCC	3 cut(s)	606, 1236, 1517
Bso31I	GGTCTC	3 cut(s)	458, 989, 1697
BsoBI	CYCGRG	3 cut(s)	526, 585, 1265
Bsp119I	TTCGAA	1 cut(s)	1506
Bsp1286I	GDGCHC	4 cut(s)	339, 435, 1543, 1819
Bsp19I	CCATGG	1 cut(s)	1237
BspACI	CCGC	9 cut(s)	92, 339, 471, 546, 711, 989, 1166, 1257, 1955
BspANI	GGCC	3 cut(s)	606, 1236, 1517
BspCNI	CTCAG	6 cut(s)	204, 421, 1033, 1375, 1459, 1979
BspFNI	CGCG	1 cut(s)	473
BspHI	TCATGA	1 cut(s)	1347
BspLI	GGNNCC	1 cut(s)	1343
BspMI	ACCTGC	2 cut(s)	1297, 1321
BspPI	GGATC	6 cut(s)	143, 248, 476, 1434, 1559, 1946
BspT104I	TTCGAA	1 cut(s)	1506
BspTNI	GGTCTC	3 cut(s)	458, 989, 1697
BsrBI	CCGCTC	1 cut(s)	1957
BsrDI	GCAATG	1 cut(s)	81
BsrI	ACTGG	5 cut(s)	25, 1001, 1122, 1478, 1627
BssECI	CCNNGG	5 cut(s)	585, 726, 836, 1237, 1764
BssNI	GRCGYC	1 cut(s)	618
BssSI	CACGAG	2 cut(s)	104, 137
BssT1I	CCWWGG	2 cut(s)	836, 1237
Bst2BI	CACGAG	2 cut(s)	104, 137
Bst2UI	CCWGG	1 cut(s)	232
Bst4CI	ACNGT	3 cut(s)	682, 1318, 1932
Bst6I	CTCTTC	2 cut(s)	261, 672
BstACI	GRCGYC	1 cut(s)	618
BstAPI	GCANNNNNTGC	2 cut(s)	23, 227
BstBAI	YACGTR	2 cut(s)	757, 1299
BstBI	TTCGAA	1 cut(s)	1506
BstC8I	GCNNGC	4 cut(s)	339, 1159, 1259, 1788
BstDSI	CCRYGG	3 cut(s)	726, 1237, 1764
BstENI	CCTNNNNNAGG	1 cut(s)	235
BstF5I	GGATG	3 cut(s)	504, 658, 1907
BstFNI	CGCG	1 cut(s)	473
BstHHI	GCGC	2 cut(s)	40, 405
BstMAI	GTCTC	5 cut(s)	121, 458, 989, 1645, 1697
BstMWI	GCNNNNNNNGC	9 cut(s)	23, 227, 569, 624, 1163, 1283, 1559, 1616, 1796
BstNI	CCWGG	1 cut(s)	232
BstNSI	RCATGY	3 cut(s)	800, 1161, 1296
BstSCI	CCNGG	4 cut(s)	230, 584, 585, 670
BstSFI	CTRYAG	1 cut(s)	1450
BstSLI	GKGCMC	1 cut(s)	339
BstSNI	TACGTA	1 cut(s)	1299
BstUI	CGCG	1 cut(s)	473
BstV1I	GCAGC	9 cut(s)	233, 379, 831, 853, 913, 1266, 1597, 1675, 2029
BstV2I	GAAGAC	1 cut(s)	1358
BstX2I	RGATCY	2 cut(s)	1564, 1951
BstXI	CCANNNNNNTGG	2 cut(s)	614, 1886
BstYI	RGATCY	2 cut(s)	1564, 1951
BsuI	GTATCC	2 cut(s)	1235, 1932
BsuRI	GGCC	3 cut(s)	606, 1236, 1517
BtgI	CCRYGG	3 cut(s)	726, 1237, 1764
BtrI	CACGTC	1 cut(s)	67
BtsCI	GGATG	3 cut(s)	504, 658, 1907
BtsI	GCAGTG	3 cut(s)	921, 946, 1621
BtsIMutI	CAGTG	6 cut(s)	921, 946, 1008, 1314, 1621, 1872
BveI	ACCTGC	2 cut(s)	1297, 1321
Cac8I	GCNNGC	4 cut(s)	339, 1159, 1259, 1788
CciI	TCATGA	1 cut(s)	1347
CfoI	GCGC	2 cut(s)	40, 405
Cfr13I	GGNCC	1 cut(s)	1515
Cfr9I	CCCGGG	1 cut(s)	585
CseI	GACGC	1 cut(s)	607
Csp6I	GTAC	3 cut(s)	414, 1012, 1296
CspCI	CAANNNNNGTGG	2 cut(s)	359, 394
CviQI	GTAC	3 cut(s)	414, 1012, 1296
DraIII	CACNNNGTG	1 cut(s)	139
EaeI	YGGCCR	2 cut(s)	604, 1234
Eam1104I	CTCTTC	2 cut(s)	261, 672
EarI	CTCTTC	2 cut(s)	261, 672
EciI	GGCGGA	1 cut(s)	561
Ecl136II	GAGCTC	2 cut(s)	433, 1817
Eco105I	TACGTA	1 cut(s)	1299
Eco130I	CCWWGG	2 cut(s)	836, 1237
Eco24I	GRGCYC	2 cut(s)	435, 1819
Eco31I	GGTCTC	3 cut(s)	458, 989, 1697
Eco53kI	GAGCTC	2 cut(s)	433, 1817
Eco57I	CTGAAG	2 cut(s)	1167, 1176
Eco72I	CACGTG	1 cut(s)	757
Eco88I	CYCGRG	3 cut(s)	526, 585, 1265
EcoICRI	GAGCTC	2 cut(s)	433, 1817
EcoNI	CCTNNNNNAGG	1 cut(s)	235
EcoRII	CCWGG	1 cut(s)	230
EcoT14I	CCWWGG	2 cut(s)	836, 1237
EcoT22I	ATGCAT	2 cut(s)	1159, 1864
EcoT38I	GRGCYC	2 cut(s)	435, 1819
ErhI	CCWWGG	2 cut(s)	836, 1237
FalI	AAGNNNNNCTT	4 cut(s)	700, 732, 1779, 1811
FaqI	GGGAC	1 cut(s)	1322
FauI	CCCGC	4 cut(s)	85, 346, 1159, 1250
FbaI	TGATCA	5 cut(s)	655, 751, 1378, 1437, 1804
FblI	GTMKAC	2 cut(s)	949, 1030
Fnu4HI	GCNGC	9 cut(s)	222, 368, 842, 845, 927, 1255, 1611, 1664, 2018
FokI	GGATG	3 cut(s)	491, 665, 1914
FriOI	GRGCYC	2 cut(s)	435, 1819
Fsp4HI	GCNGC	9 cut(s)	222, 368, 842, 845, 927, 1255, 1611, 1664, 2018
FspAI	RTGCGCAY	1 cut(s)	404
FspBI	CTAG	2 cut(s)	144, 837
FspI	TGCGCA	1 cut(s)	404
GlaI	GCGC	2 cut(s)	39, 404
GluI	GCNGC	9 cut(s)	222, 368, 842, 845, 927, 1255, 1611, 1664, 2018
HaeIII	GGCC	3 cut(s)	606, 1236, 1517
HapII	CCGG	3 cut(s)	586, 671, 1219
HgaI	GACGC	1 cut(s)	607
HhaI	GCGC	2 cut(s)	40, 405
Hin1I	GRCGYC	1 cut(s)	618
Hin6I	GCGC	2 cut(s)	38, 403
HinP1I	GCGC	2 cut(s)	38, 403
HincII	GTYRAC	1 cut(s)	950
HindII	GTYRAC	1 cut(s)	950
HindIII	AAGCTT	2 cut(s)	1391, 1788
HinfI	GANTC	7 cut(s)	114, 890, 1018, 1068, 1735, 1777, 1964
HpaII	CCGG	3 cut(s)	586, 671, 1219
HphI	GGTGA	5 cut(s)	125, 514, 698, 1088, 1584
Hpy166II	GTNNAC	6 cut(s)	201, 295, 730, 950, 962, 1031
Hpy188III	TCNNGA	7 cut(s)	599, 909, 971, 1265, 1348, 1398, 1988
Hpy8I	GTNNAC	6 cut(s)	201, 295, 730, 950, 962, 1031
Hpy99I	CGWCG	3 cut(s)	165, 605, 951
HpyAV	CCTTC	5 cut(s)	184, 803, 1298, 1568, 1670
HpyCH4III	ACNGT	3 cut(s)	682, 1318, 1932
HpyCH4IV	ACGT	3 cut(s)	66, 756, 1298
HpyF10VI	GCNNNNNNNGC	9 cut(s)	23, 227, 569, 624, 1163, 1283, 1559, 1616, 1796
HpySE526I	ACGT	3 cut(s)	66, 756, 1298
Hsp92I	GRCGYC	1 cut(s)	618
HspAI	GCGC	2 cut(s)	38, 403
Ksp22I	TGATCA	5 cut(s)	655, 751, 1378, 1437, 1804
LmnI	GCTCC	4 cut(s)	48, 364, 713, 2025
Lsp1109I	GCAGC	9 cut(s)	233, 379, 831, 853, 913, 1266, 1597, 1675, 2029
LweI	GCATC	4 cut(s)	390, 1738, 1849, 1892
MaeI	CTAG	2 cut(s)	144, 837
MaeII	ACGT	3 cut(s)	66, 756, 1298
MaeIII	GTNAC	2 cut(s)	799, 1972
MbiI	CCGCTC	1 cut(s)	1957
MfeI	CAATTG	3 cut(s)	897, 1614, 1697
MflI	RGATCY	2 cut(s)	1564, 1951
MhlI	GDGCHC	4 cut(s)	339, 435, 1543, 1819
MlsI	TGGCCA	1 cut(s)	1236
MluNI	TGGCCA	1 cut(s)	1236
MlyI	GAGTC	3 cut(s)	123, 1012, 1958
MmeI	TCCRAC	1 cut(s)	509
Mox20I	TGGCCA	1 cut(s)	1236
Mph1103I	ATGCAT	2 cut(s)	1159, 1864
MscI	TGGCCA	1 cut(s)	1236
MseI	TTAA	2 cut(s)	978, 1866
MslI	CAYNNNNRTG	3 cut(s)	280, 1291, 1523
Msp20I	TGGCCA	1 cut(s)	1236
MspA1I	CMGCKG	3 cut(s)	844, 1196, 1257
MspI	CCGG	3 cut(s)	586, 671, 1219
MspR9I	CCNGG	4 cut(s)	232, 586, 587, 672
MunI	CAATTG	3 cut(s)	897, 1614, 1697
Mva1269I	GAATGC	1 cut(s)	1220
MvaI	CCWGG	1 cut(s)	232
MvnI	CGCG	1 cut(s)	473
MwoI	GCNNNNNNNGC	9 cut(s)	23, 227, 569, 624, 1163, 1283, 1559, 1616, 1796
NciI	CCSGG	3 cut(s)	586, 587, 672
NcoI	CCATGG	1 cut(s)	1237
NlaIV	GGNNCC	1 cut(s)	1343
NmuCI	GTSAC	1 cut(s)	799
NsbI	TGCGCA	1 cut(s)	404
NsiI	ATGCAT	2 cut(s)	1159, 1864
NspI	RCATGY	3 cut(s)	800, 1161, 1296
NspV	TTCGAA	1 cut(s)	1506
PaeI	GCATGC	1 cut(s)	1161
PaeR7I	CTCGAG	1 cut(s)	526
PagI	TCATGA	1 cut(s)	1347
PaqCI	CACCTGC	1 cut(s)	1297
PciI	ACATGT	1 cut(s)	796
PctI	GAATGC	1 cut(s)	1220
PfeI	GAWTC	4 cut(s)	890, 1068, 1735, 1777
PkrI	GCNGC	9 cut(s)	223, 369, 843, 846, 928, 1256, 1612, 1665, 2019
PleI	GAGTC	3 cut(s)	122, 1012, 1958
PmaCI	CACGTG	1 cut(s)	757
PmlI	CACGTG	1 cut(s)	757
PpsI	GAGTC	3 cut(s)	122, 1012, 1958
Ppu21I	YACGTR	2 cut(s)	757, 1299
PscI	ACATGT	1 cut(s)	796
PshBI	ATTAAT	1 cut(s)	978
Psp124BI	GAGCTC	2 cut(s)	435, 1819
Psp6I	CCWGG	1 cut(s)	230
PspCI	CACGTG	1 cut(s)	757
PspGI	CCWGG	1 cut(s)	230
PspN4I	GGNNCC	1 cut(s)	1343
PspPI	GGNCC	1 cut(s)	1515
PspXI	VCTCGAGB	1 cut(s)	526
PsuI	RGATCY	2 cut(s)	1564, 1951
PvuII	CAGCTG	2 cut(s)	844, 1196
RsaI	GTAC	3 cut(s)	415, 1013, 1297
RsaNI	GTAC	3 cut(s)	414, 1012, 1296
RseI	CAYNNNNRTG	3 cut(s)	280, 1291, 1523
SacI	GAGCTC	2 cut(s)	435, 1819
SalI	GTCGAC	1 cut(s)	948
SaqAI	TTAA	2 cut(s)	978, 1866
SatI	GCNGC	9 cut(s)	222, 368, 842, 845, 927, 1255, 1611, 1664, 2018
Sau96I	GGNCC	1 cut(s)	1515
SchI	GAGTC	3 cut(s)	123, 1012, 1958
ScrFI	CCNGG	4 cut(s)	232, 586, 587, 672
SduI	GDGCHC	4 cut(s)	339, 435, 1543, 1819
SfaNI	GCATC	4 cut(s)	390, 1738, 1849, 1892
SfcI	CTRYAG	1 cut(s)	1450
Sfr274I	CTCGAG	1 cut(s)	526
SfuI	TTCGAA	1 cut(s)	1506
SlaI	CTCGAG	1 cut(s)	526
SmaI	CCCGGG	1 cut(s)	587
SmiMI	CAYNNNNRTG	3 cut(s)	280, 1291, 1523
SmlI	CTYRAG	2 cut(s)	526, 1496
SmoI	CTYRAG	2 cut(s)	526, 1496
SnaBI	TACGTA	1 cut(s)	1299
SphI	GCATGC	1 cut(s)	1161
SsiI	CCGC	9 cut(s)	92, 339, 471, 546, 711, 989, 1166, 1257, 1955
SspI	AATATT	2 cut(s)	1445, 1745
SspMI	CTAG	2 cut(s)	144, 837
SstI	GAGCTC	2 cut(s)	435, 1819
StyD4I	CCNGG	4 cut(s)	230, 584, 585, 670
StyI	CCWWGG	2 cut(s)	836, 1237
TaaI	ACNGT	3 cut(s)	682, 1318, 1932
TaiI	ACGT	3 cut(s)	69, 759, 1301
TatI	WGTACW	1 cut(s)	1011
TfiI	GAWTC	4 cut(s)	890, 1068, 1735, 1777
Tru1I	TTAA	2 cut(s)	978, 1866
Tru9I	TTAA	2 cut(s)	978, 1866
TscAI	CASTG	6 cut(s)	921, 946, 1008, 1321, 1628, 1879
TseFI	GTSAC	1 cut(s)	799
TseI	GCWGC	9 cut(s)	221, 367, 841, 844, 926, 1254, 1610, 1663, 2017
Tsp45I	GTSAC	1 cut(s)	799
TspGWI	ACGGA	3 cut(s)	374, 715, 1698
TspMI	CCCGGG	1 cut(s)	585
TspRI	CASTG	6 cut(s)	921, 946, 1008, 1321, 1628, 1879
VspI	ATTAAT	1 cut(s)	978
XagI	CCTNNNNNAGG	1 cut(s)	235
XapI	RAATTY	3 cut(s)	639, 701, 1646
XceI	RCATGY	3 cut(s)	800, 1161, 1296
XhoI	CTCGAG	1 cut(s)	526
XmaI	CCCGGG	1 cut(s)	585
XmaJI	CCTAGG	1 cut(s)	836
XmiI	GTMKAC	2 cut(s)	949, 1030
XspI	CTAG	2 cut(s)	144, 837
Zsp2I	ATGCAT	2 cut(s)	1159, 1864

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.