MD04G1068300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013961
Physical Location & Seq
Forward (+)
9287425 .. 9288287
863 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1068300.v1.1.491

Sequence Viewer

Length: 786 bp
ATGGATTTTCGAAAACCCAATGCGATCCAGCAGAGGCCGGTGCTGCTGAAAGATTTTCTGATGGACGATCTCAGCTCCTGCTCATCCAACGGCTTCAAATCCTTCCCTCGCCGCCAGTTCTGCACAACCACCGTCCGCTTCCTCCTCGACATCGAATTCAAGAGCTCATCGTCGTCGTCTCAACTGTCCACTCACAAGTATCCCAGTAGGATTACTTCGCAAAAGCCAGTCCTCCACCGAAGCCGGTCCAGGGCCGCCGCCGCTTGCGACGCCTTCATTAACGCCGTCAAGAGCCTCCCTTCCCCCCCGTCCGTACGGAGAGGAGTGGTCTTGCTTCTTCCGAAAAGCCTTTCCCGGCGGCTGCGGAGGAAGAGATTCTGGAAAAAACAGAGCAAGGAAGAGAAGAAAGAAGAGAATGACATCGGACGGTGGAGATTGTTCAGTGAGTTCCTCCACGACGAACCTCCTCCGTCCGATCAAAACACCACCACCACAGCTAAAACCGTAGTCACGGTCTCCAGACATTCTACCAGTACCAGAAGCCGTGGAAGTGATGGTAACAGTAGCAGCAGTAACAGTTGGGCGGAGAGCGAGTTTACTTTGAGCTCCCAGAGTTCAGGTGGAAACGACGGCGTGGATGTCAAAACGACGAATTTAGTGAAAGACAAAGTCAGCGAGAAAGTTGGCGGGGAAGTGGGTGAGGGGGAAGTGGGTGAGGACTCCGTTTCCATGGCATGGCCAACCACCACCACCTCCTGCTCACCTCAAAACGATAAGGTACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

29.03

Weight (kDa)

9.82

Isoelectric Point (pI)

70.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013961)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11780 AT4G23020 AT4G23020
fragaria_vesca FvH4_5g01430
malus_domestica MD04G1068300.v1.1
prunus_persica Prupe.5G089800_v2.0.a1
pyrus_communis pycom04g06170
rosa_chinensis RchiOBHm_Chr7g0199421
rosa_laevigata RLG00000003794
rosa_multiflora Rmu_sc0003890.1_g000015
rosa_roxburghii Rroxscaffold_3G00256740
rosa_rugosa Rorug07G0053800
rosa_samantha Rh7AG178900 Rh7BG182300 Rh7CG187500 Rh7DG181800
rosa_wichuraiana Rw7G015710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 735
AciI CCGC 9 cut(s) 112, 136, 255, 258, 261, 358, 364, 584, 687
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 1 cut(s) 737
AcsI RAATTY 2 cut(s) 155, 652
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 3 cut(s) 315, 535, 780
AfiI CCNNNNNNNGG 2 cut(s) 250, 735
AgsI TTSAA 2 cut(s) 97, 160
AjnI CCWGG 1 cut(s) 248
AluBI AGCT 4 cut(s) 75, 165, 497, 606
AluI AGCT 4 cut(s) 75, 165, 497, 606
Alw21I GWGCWC 2 cut(s) 167, 608
Alw26I GTCTC 2 cut(s) 183, 520
AlwI GGATC 1 cut(s) 19
AlwNI CAGNNNCTG 1 cut(s) 78
AoxI GGCC 3 cut(s) 35, 252, 737
ApeKI GCWGC 3 cut(s) 43, 361, 567
ApoI RAATTY 2 cut(s) 155, 652
Asp700I GAANNNNTTC 1 cut(s) 374
AspS9I GGNCC 2 cut(s) 246, 252
AsuC2I CCSGG 1 cut(s) 355
AsuHPI GGTGA 3 cut(s) 710, 725, 753
AsuII TTCGAA 1 cut(s) 10
AvaII GGWCC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 739
BanII GRGCYC 2 cut(s) 167, 608
BarI GAAGNNNNNNTAC 2 cut(s) 199, 231
Bbv12I GWGCWC 2 cut(s) 167, 608
BbvI GCAGC 3 cut(s) 30, 348, 579
BccI CCATC 2 cut(s) 55, 548
BceAI ACGGC 4 cut(s) 106, 269, 528, 646
BciT130I CCWGG 1 cut(s) 250
BciVI GTATCC 1 cut(s) 210
BcnI CCSGG 1 cut(s) 355
BcoDI GTCTC 2 cut(s) 183, 520
BfuI GTATCC 1 cut(s) 210
BisI GCNGC 8 cut(s) 44, 112, 255, 258, 261, 359, 362, 568
BlsI GCNGC 8 cut(s) 45, 113, 256, 259, 262, 360, 363, 569
Bme1390I CCNGG 2 cut(s) 250, 355
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 2 cut(s) 246, 252
BmrFI CCNGG 2 cut(s) 250, 355
BmrI ACTGGG 1 cut(s) 198
BmuI ACTGGG 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 502
Bpu14I TTCGAA 1 cut(s) 10
BpuMI CCSGG 1 cut(s) 355
BsaHI GRCGYC 1 cut(s) 270
BsaI GGTCTC 1 cut(s) 520
BsaJI CCNNGG 3 cut(s) 249, 544, 729
BsaXI ACNNNNNCTCC 2 cut(s) 578, 608
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 735
Bse118I RCCGGY 2 cut(s) 37, 243
Bse1I ACTGG 4 cut(s) 115, 204, 227, 531
BseBI CCWGG 1 cut(s) 250
BseDI CCNNGG 3 cut(s) 249, 544, 729
BseGI GGATG 2 cut(s) 83, 643
BseLI CCNNNNNNNGG 2 cut(s) 250, 735
BseMII CTCAG 1 cut(s) 85
BseNI ACTGG 4 cut(s) 115, 204, 227, 531
BseRI GAGGAG 3 cut(s) 134, 336, 456
BseXI GCAGC 3 cut(s) 30, 348, 579
BsgI GTGCAG 1 cut(s) 106
BshFI GGCC 3 cut(s) 37, 254, 739
BsiHKAI GWGCWC 2 cut(s) 167, 608
BsiSI CCGG 3 cut(s) 38, 244, 355
BsiWI CGTACG 1 cut(s) 313
BslI CCNNNNNNNGG 2 cut(s) 250, 735
BsmAI GTCTC 2 cut(s) 183, 520
BsmBI CGTCTC 1 cut(s) 183
BsnI GGCC 3 cut(s) 37, 254, 739
Bso31I GGTCTC 1 cut(s) 520
Bsp119I TTCGAA 1 cut(s) 10
Bsp1286I GDGCHC 2 cut(s) 167, 608
Bsp143I GATC 3 cut(s) 24, 67, 475
Bsp19I CCATGG 1 cut(s) 729
BspACI CCGC 9 cut(s) 112, 136, 255, 258, 261, 358, 364, 584, 687
BspANI GGCC 3 cut(s) 37, 254, 739
BspCNI CTCAG 1 cut(s) 84
BspPI GGATC 1 cut(s) 19
BspT104I TTCGAA 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 520
BsrFI RCCGGY 2 cut(s) 37, 243
BsrI ACTGG 4 cut(s) 115, 204, 227, 531
BssAI RCCGGY 2 cut(s) 37, 243
BssECI CCNNGG 3 cut(s) 249, 544, 729
BssMI GATC 3 cut(s) 24, 67, 475
BssNI GRCGYC 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 729
Bst2UI CCWGG 1 cut(s) 250
Bst4CI ACNGT 7 cut(s) 133, 186, 429, 505, 514, 563, 578
Bst6I CTCTTC 3 cut(s) 365, 393, 405
BstACI GRCGYC 1 cut(s) 270
BstBI TTCGAA 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 265
BstDEI CTNAG 1 cut(s) 71
BstDSI CCRYGG 2 cut(s) 544, 729
BstF5I GGATG 2 cut(s) 83, 643
BstKTI GATC 3 cut(s) 27, 70, 478
BstMAI GTCTC 2 cut(s) 183, 520
BstMBI GATC 3 cut(s) 24, 67, 475
BstMWI GCNNNNNNNGC 4 cut(s) 43, 120, 260, 269
BstNI CCWGG 1 cut(s) 250
BstSCI CCNGG 2 cut(s) 248, 353
BstV1I GCAGC 3 cut(s) 30, 348, 579
BsuI GTATCC 1 cut(s) 210
BsuRI GGCC 3 cut(s) 37, 254, 739
BtgI CCRYGG 2 cut(s) 544, 729
BtsCI GGATG 2 cut(s) 83, 643
BtsIMutI CAGTG 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 265
CaiI CAGNNNCTG 1 cut(s) 78
Cfr10I RCCGGY 2 cut(s) 37, 243
Cfr13I GGNCC 2 cut(s) 246, 252
CseI GACGC 1 cut(s) 278
Csp6I GTAC 3 cut(s) 314, 534, 779
CviAII CATG 2 cut(s) 730, 735
CviQI GTAC 3 cut(s) 314, 534, 779
DdeI CTNAG 1 cut(s) 71
DpnI GATC 3 cut(s) 26, 69, 477
DpnII GATC 3 cut(s) 24, 67, 475
EaeI YGGCCR 1 cut(s) 737
Eam1104I CTCTTC 3 cut(s) 365, 393, 405
EarI CTCTTC 3 cut(s) 365, 393, 405
EciI GGCGGA 1 cut(s) 599
Ecl136II GAGCTC 2 cut(s) 165, 606
Eco130I CCWWGG 1 cut(s) 729
Eco24I GRGCYC 2 cut(s) 167, 608
Eco31I GGTCTC 1 cut(s) 520
Eco47I GGWCC 1 cut(s) 246
Eco53kI GAGCTC 2 cut(s) 165, 606
EcoICRI GAGCTC 2 cut(s) 165, 606
EcoRI GAATTC 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 729
EcoT38I GRGCYC 2 cut(s) 167, 608
ErhI CCWWGG 1 cut(s) 729
Esp3I CGTCTC 1 cut(s) 183
FaeI CATG 2 cut(s) 733, 738
FaiI YATR 2 cut(s) 731, 736
FatI CATG 2 cut(s) 729, 734
FauI CCCGC 1 cut(s) 680
Fnu4HI GCNGC 8 cut(s) 44, 112, 255, 258, 261, 359, 362, 568
FokI GGATG 2 cut(s) 70, 650
FriOI GRGCYC 2 cut(s) 167, 608
Fsp4HI GCNGC 8 cut(s) 44, 112, 255, 258, 261, 359, 362, 568
GluI GCNGC 8 cut(s) 44, 112, 255, 258, 261, 359, 362, 568
GsuI CTGGAG 1 cut(s) 502
HaeIII GGCC 3 cut(s) 37, 254, 739
HapII CCGG 3 cut(s) 38, 244, 355
HgaI GACGC 1 cut(s) 278
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 2 cut(s) 733, 738
HinfI GANTC 2 cut(s) 375, 719
HpaII CCGG 3 cut(s) 38, 244, 355
HphI GGTGA 3 cut(s) 710, 725, 753
Hpy166II GTNNAC 2 cut(s) 189, 597
Hpy188I TCNGA 4 cut(s) 60, 342, 425, 475
Hpy188III TCNNGA 4 cut(s) 160, 289, 379, 519
Hpy8I GTNNAC 2 cut(s) 189, 597
Hpy99I CGWCG 6 cut(s) 175, 178, 272, 461, 632, 652
HpyAV CCTTC 3 cut(s) 112, 283, 309
HpyCH4III ACNGT 7 cut(s) 133, 186, 429, 505, 514, 563, 578
HpyCH4IV ACGT 1 cut(s) 781
HpyCH4V TGCA 1 cut(s) 123
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 120, 260, 269
HpyF3I CTNAG 1 cut(s) 71
HpySE526I ACGT 1 cut(s) 781
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 2 cut(s) 733, 738
Kzo9I GATC 3 cut(s) 24, 67, 475
LmnI GCTCC 2 cut(s) 80, 611
Lsp1109I GCAGC 3 cut(s) 30, 348, 579
MaeII ACGT 1 cut(s) 781
MaeIII GTNAC 3 cut(s) 508, 557, 572
MalI GATC 3 cut(s) 26, 69, 477
MboI GATC 3 cut(s) 24, 67, 475
MboII GAAGA 5 cut(s) 329, 382, 410, 415, 422
MhlI GDGCHC 2 cut(s) 167, 608
MlsI TGGCCA 1 cut(s) 739
MluCI AATT 2 cut(s) 155, 652
MluNI TGGCCA 1 cut(s) 739
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 1 cut(s) 111
Mox20I TGGCCA 1 cut(s) 739
MroXI GAANNNNTTC 1 cut(s) 374
MscI TGGCCA 1 cut(s) 739
MseI TTAA 2 cut(s) 279, 784
Msp20I TGGCCA 1 cut(s) 739
MspI CCGG 3 cut(s) 38, 244, 355
MspR9I CCNGG 2 cut(s) 250, 355
MvaI CCWGG 1 cut(s) 250
MwoI GCNNNNNNNGC 4 cut(s) 43, 120, 260, 269
NciI CCSGG 1 cut(s) 355
NcoI CCATGG 1 cut(s) 729
NdeII GATC 3 cut(s) 24, 67, 475
NlaIII CATG 2 cut(s) 733, 738
NmuCI GTSAC 1 cut(s) 508
NspV TTCGAA 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 1 cut(s) 375
Pfl23II CGTACG 1 cut(s) 313
PflFI GACNNNGTC 1 cut(s) 668
PflMI CCANNNNNTGG 1 cut(s) 735
PkrI GCNGC 8 cut(s) 45, 113, 256, 259, 262, 360, 363, 569
PleI GAGTC 1 cut(s) 713
PpsI GAGTC 1 cut(s) 713
Psp124BI GAGCTC 2 cut(s) 167, 608
Psp6I CCWGG 1 cut(s) 248
PspGI CCWGG 1 cut(s) 248
PspLI CGTACG 1 cut(s) 313
PspPI GGNCC 2 cut(s) 246, 252
PstNI CAGNNNCTG 1 cut(s) 78
PsyI GACNNNGTC 1 cut(s) 668
RsaI GTAC 3 cut(s) 315, 535, 780
RsaNI GTAC 3 cut(s) 314, 534, 779
SacI GAGCTC 2 cut(s) 167, 608
SaqAI TTAA 2 cut(s) 279, 784
SatI GCNGC 8 cut(s) 44, 112, 255, 258, 261, 359, 362, 568
Sau3AI GATC 3 cut(s) 24, 67, 475
Sau96I GGNCC 2 cut(s) 246, 252
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 2 cut(s) 250, 355
SduI GDGCHC 2 cut(s) 167, 608
SfuI TTCGAA 1 cut(s) 10
SinI GGWCC 1 cut(s) 246
Sse9I AATT 2 cut(s) 155, 652
SsiI CCGC 9 cut(s) 112, 136, 255, 258, 261, 358, 364, 584, 687
SstI GAGCTC 2 cut(s) 167, 608
StyD4I CCNGG 2 cut(s) 248, 353
StyI CCWWGG 1 cut(s) 729
TaaI ACNGT 7 cut(s) 133, 186, 429, 505, 514, 563, 578
TaiI ACGT 1 cut(s) 784
TaqI TCGA 3 cut(s) 10, 147, 153
TasI AATT 2 cut(s) 155, 652
TauI GCSGC 5 cut(s) 114, 257, 260, 263, 361
TfiI GAWTC 1 cut(s) 375
Tru1I TTAA 2 cut(s) 279, 784
Tru9I TTAA 2 cut(s) 279, 784
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 3 cut(s) 43, 361, 567
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 1 cut(s) 265
TspGWI ACGGA 4 cut(s) 301, 331, 459, 712
TspRI CASTG 1 cut(s) 448
Tth111I GACNNNGTC 1 cut(s) 668
Van91I CCANNNNNTGG 1 cut(s) 735
VpaK11BI GGWCC 1 cut(s) 246
XapI RAATTY 2 cut(s) 155, 652
XcmI CCANNNNNNNNNTGG 1 cut(s) 617
XmnI GAANNNNTTC 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.