RLG00000003794

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013961
Physical Location & Seq
Reverse (-)
52812025 .. 52816032
4008 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003794

Sequence Viewer

Length: 1434 bp
ATGGCTTTAGTAACTCCACCGTGCTTGGAAATTCAAAGGCCGAGAAAATCAGCTATGATTCAGAAGAAGCCGTTGATGCTCAAAGATTATCTGATGGACGATCTGAGCTCCTGCTCATCCAGCGGCTTCAAATCCTTCCCACGCCTCCAATGCTGCACAACCGTCCGATTTCTCCTCGAGATCGACCTCAAATCCTCCTCCCGGCCACAACTGCCGCCGCGGCAGCCCATTCACAACCACAACAACTTCAAGAATCAAAAGCCAAAGCAACTACTCAAACGGAGCCGGTCCAGAGCCACCGCCTCCAACACCCTCTCCGCTCTCCAAAGAGCTTCCGGCTCCGTCATTAACGCCGTCAGAAACTTGCAGTTTCACTCCATCAAGTCCACCTCGTCGTCTGTACAAAGCAGTGCTCGGAAAGGCGGCTTGGTACTGCTTCTGCCTAGAAGCATTTCACGGCGGCTGTGGAGGAAGAGCTTCCGGACAAAAGTAGAAGAAGAAGAGAGAGACATAGTAAAGTGGAGATCGTTCCGGGAACTTCTGCAGGAGCAAGATAAGCCGTCCGATCAGACCACCGCCAGTACAAAAAGTACCGACAGTAAAACGAACAGCAACAGTTGGGGGGAGAGCGAGTTTAGTTTGACCTCGGAGAGTTCAATTGGCAACGACGTGGCGGAAGGTAAAAATAGTTTACCAGAAACTAAGAAAGTCGGCGGAAGAGTTGGCGTAACAGCCGGTGAGGATTCCATTGAAACACCAACCACCACCACCTCCACCTGCAACCCTCAAAACGCAAAGGTGTGGCTGAATAAAGAGGAGGAGAAAGAACAGTTTAGTCCCGTGTCGGTGCTCGATTGTCCATTTGAGGATGAAGATGATGAGAGTAGTAGTAGCTCTCCTTTTCGTGATAGACTTGCTCGAATGGAAGGTACACAACACAAACTCATGCAGAAGATCAGACGATTCGAGAACCTCGCTCAACTGGAGCCAATCGACTTGGAAAGACGAATGGCAATGTCAGAGACAGATGGAGATGACCGAGATAACAGAACCGAAGAGAAAGCCGGAGAGCTAGTGAAGCGGTTGATCATCAAAACTAGTAGCTCTGCCCTATCGAACGATTTGGTAGTGCATATGAAGGCCGATAAGTTGGTGGTGGATTTGTTCAGAGAGAGGAGTGTCGAAAACAGCAAGGAAAATGTGGTGATGGGAGAGGTACTGAAAGTAGCAGAAGAGTGGGTGAATGGGCAATCGCCAATGTTGTCGCGTTGGGAGGTCGAAAATGGTAGAAAAGTTTATGTTAATGATATGGACAAGAAGTTCTGTGGCAAGTGGATGAAATTGGATGAACAAGAAAAAGAGCTTGGTTTGGAGTTGGAGGTTGAGGTATGGAATTCTTTGCTGAATGAATTCCTGCTTGATCTAGTTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

478

Amino Acids

54.18

Weight (kDa)

8.04

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013961)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11780 AT4G23020 AT4G23020
fragaria_vesca FvH4_5g01430
malus_domestica MD04G1068300.v1.1
prunus_persica Prupe.5G089800_v2.0.a1
pyrus_communis pycom04g06170
rosa_chinensis RchiOBHm_Chr7g0199421
rosa_laevigata RLG00000003794
rosa_multiflora Rmu_sc0003890.1_g000015
rosa_roxburghii Rroxscaffold_3G00256740
rosa_rugosa Rorug07G0053800
rosa_samantha Rh7AG178900 Rh7BG182300 Rh7CG187500 Rh7DG181800
rosa_wichuraiana Rw7G015710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 785
Acc36I ACCTGC 1 cut(s) 785
AccBSI CCGCTC 1 cut(s) 320
AccII CGCG 2 cut(s) 220, 1267
AccIII TCCGGA 1 cut(s) 480
AcoI YGGCCR 1 cut(s) 203
AcsI RAATTY 3 cut(s) 30, 1393, 1409
AfaI GTAC 6 cut(s) 402, 432, 583, 592, 931, 1218
AfiI CCNNNNNNNGG 1 cut(s) 201
AgsI TTSAA 5 cut(s) 35, 130, 250, 657, 752
AhlI ACTAGT 1 cut(s) 1097
AjiI CACGTC 1 cut(s) 670
AjuI GAANNNNNNNTTGG 4 cut(s) 410, 442, 1347, 1379
AloI GAACNNNNNNTCC 2 cut(s) 1304, 1336
AluBI AGCT 9 cut(s) 53, 108, 332, 477, 894, 1072, 1104, 1363, 1431
AluI AGCT 9 cut(s) 53, 108, 332, 477, 894, 1072, 1104, 1363, 1431
Alw21I GWGCWC 3 cut(s) 110, 415, 852
Alw26I GTCTC 2 cut(s) 501, 1016
Ama87I CYCGRG 1 cut(s) 176
Aor13HI TCCGGA 1 cut(s) 480
AoxI GGCC 3 cut(s) 38, 203, 1140
ApeKI GCWGC 2 cut(s) 153, 223
ApoI RAATTY 3 cut(s) 30, 1393, 1409
Asp700I GAANNNNTTC 3 cut(s) 451, 476, 1409
AspS9I GGNCC 1 cut(s) 288
AsuC2I CCSGG 2 cut(s) 202, 533
AsuHPI GGTGA 3 cut(s) 749, 1216, 1252
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 288
BanII GRGCYC 1 cut(s) 110
Bbv12I GWGCWC 3 cut(s) 110, 415, 852
BbvI GCAGC 2 cut(s) 140, 235
BccI CCATC 4 cut(s) 88, 386, 1022, 1201
BceAI ACGGC 4 cut(s) 55, 338, 473, 544
BclI TGATCA 1 cut(s) 1086
BcnI CCSGG 2 cut(s) 202, 533
BcoDI GTCTC 2 cut(s) 501, 1016
BcuI ACTAGT 1 cut(s) 1097
BfaI CTAG 5 cut(s) 444, 1073, 1098, 1424, 1432
BfmI CTRYAG 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 785
BglI GCCNNNNNGGC 1 cut(s) 220
BisI GCNGC 8 cut(s) 124, 154, 215, 218, 221, 224, 424, 461
BlsI GCNGC 8 cut(s) 125, 155, 216, 219, 222, 225, 425, 462
Bme1390I CCNGG 2 cut(s) 202, 533
Bme18I GGWCC 1 cut(s) 288
BmeT110I CYCGRG 1 cut(s) 176
BmgBI CACGTC 1 cut(s) 670
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 3 cut(s) 284, 340, 987
BmrFI CCNGG 2 cut(s) 202, 533
BmsI GCATC 1 cut(s) 66
BpmI CTGGAG 1 cut(s) 1004
BpuMI CCSGG 2 cut(s) 202, 533
BsaJI CCNNGG 2 cut(s) 218, 645
BsaWI WCCGGW 1 cut(s) 480
BsaXI ACNNNNNCTCC 6 cut(s) 299, 329, 617, 647, 1059, 1089
Bsc4I CCNNNNNNNGG 1 cut(s) 201
Bse118I RCCGGY 2 cut(s) 285, 734
Bse1I ACTGG 2 cut(s) 579, 987
Bse3DI GCAATG 1 cut(s) 1020
BseAI TCCGGA 1 cut(s) 480
BseDI CCNNGG 2 cut(s) 218, 645
BseGI GGATG 4 cut(s) 116, 874, 1341, 1351
BseLI CCNNNNNNNGG 1 cut(s) 201
BseMI GCAATG 1 cut(s) 1020
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 2 cut(s) 579, 987
BseRI GAGGAG 5 cut(s) 164, 187, 830, 833, 1189
BseXI GCAGC 2 cut(s) 140, 235
BsgI GTGCAG 1 cut(s) 139
Bsh1236I CGCG 2 cut(s) 220, 1267
BshFI GGCC 3 cut(s) 40, 205, 1142
BsiHKAI GWGCWC 3 cut(s) 110, 415, 852
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 7 cut(s) 202, 286, 336, 481, 532, 735, 1065
BslFI GGGAC 1 cut(s) 822
BslI CCNNNNNNNGG 1 cut(s) 201
BsmAI GTCTC 2 cut(s) 501, 1016
BsmFI GGGAC 1 cut(s) 822
BsnI GGCC 3 cut(s) 40, 205, 1142
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 3 cut(s) 110, 415, 852
Bsp13I TCCGGA 1 cut(s) 480
Bsp1407I TGTACA 1 cut(s) 400
Bsp143I GATC 7 cut(s) 100, 180, 524, 565, 954, 1086, 1420
BspANI GGCC 3 cut(s) 40, 205, 1142
BspCNI CTCAG 1 cut(s) 96
BspEI TCCGGA 1 cut(s) 480
BspFNI CGCG 2 cut(s) 220, 1267
BspLI GGNNCC 3 cut(s) 284, 340, 987
BspMAI CTGCAG 1 cut(s) 546
BspMI ACCTGC 1 cut(s) 785
BspQI GCTCTTC 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 1020
BsrFI RCCGGY 2 cut(s) 285, 734
BsrGI TGTACA 1 cut(s) 400
BsrI ACTGG 2 cut(s) 579, 987
BssAI RCCGGY 2 cut(s) 285, 734
BssECI CCNNGG 2 cut(s) 218, 645
BssMI GATC 7 cut(s) 100, 180, 524, 565, 954, 1086, 1420
Bst4CI ACNGT 5 cut(s) 21, 163, 599, 617, 831
Bst6I CTCTTC 5 cut(s) 467, 495, 712, 1050, 1227
BstAUI TGTACA 1 cut(s) 400
BstDEI CTNAG 2 cut(s) 104, 702
BstDSI CCRYGG 1 cut(s) 218
BstF5I GGATG 4 cut(s) 116, 874, 1341, 1351
BstFNI CGCG 2 cut(s) 220, 1267
BstKTI GATC 7 cut(s) 103, 183, 527, 568, 957, 1089, 1423
BstMAI GTCTC 2 cut(s) 501, 1016
BstMBI GATC 7 cut(s) 100, 180, 524, 565, 954, 1086, 1420
BstMWI GCNNNNNNNGC 8 cut(s) 76, 120, 150, 211, 220, 223, 556, 1078
BstSCI CCNGG 2 cut(s) 200, 531
BstSFI CTRYAG 1 cut(s) 542
BstUI CGCG 2 cut(s) 220, 1267
BstV1I GCAGC 2 cut(s) 140, 235
BsuRI GGCC 3 cut(s) 40, 205, 1142
BtgI CCRYGG 1 cut(s) 218
BtrI CACGTC 1 cut(s) 670
BtsCI GGATG 4 cut(s) 116, 874, 1341, 1351
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 415
BveI ACCTGC 1 cut(s) 785
Cfr10I RCCGGY 2 cut(s) 285, 734
Cfr13I GGNCC 1 cut(s) 288
Cfr42I CCGCGG 1 cut(s) 221
Csp6I GTAC 6 cut(s) 401, 431, 582, 591, 930, 1217
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 1 cut(s) 946
CviQI GTAC 6 cut(s) 401, 431, 582, 591, 930, 1217
DdeI CTNAG 2 cut(s) 104, 702
DpnI GATC 7 cut(s) 102, 182, 526, 567, 956, 1088, 1422
DpnII GATC 7 cut(s) 100, 180, 524, 565, 954, 1086, 1420
EaeI YGGCCR 1 cut(s) 203
Eam1104I CTCTTC 5 cut(s) 467, 495, 712, 1050, 1227
EarI CTCTTC 5 cut(s) 467, 495, 712, 1050, 1227
EciI GGCGGA 2 cut(s) 689, 729
Ecl136II GAGCTC 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 108
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 108
EcoRI GAATTC 2 cut(s) 1393, 1409
EcoT38I GRGCYC 1 cut(s) 110
FaeI CATG 1 cut(s) 949
FaiI YATR 8 cut(s) 56, 512, 947, 1134, 1136, 1299, 1310, 1390
FaqI GGGAC 1 cut(s) 822
FatI CATG 1 cut(s) 945
FauNDI CATATG 1 cut(s) 1134
FbaI TGATCA 1 cut(s) 1086
Fnu4HI GCNGC 8 cut(s) 124, 154, 215, 218, 221, 224, 424, 461
FokI GGATG 4 cut(s) 103, 881, 1348, 1358
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 8 cut(s) 124, 154, 215, 218, 221, 224, 424, 461
FspBI CTAG 5 cut(s) 444, 1073, 1098, 1424, 1432
GluI GCNGC 8 cut(s) 124, 154, 215, 218, 221, 224, 424, 461
GsuI CTGGAG 1 cut(s) 1004
HaeIII GGCC 3 cut(s) 40, 205, 1142
HapII CCGG 7 cut(s) 202, 286, 336, 481, 532, 735, 1065
Hin1II CATG 1 cut(s) 949
HinfI GANTC 4 cut(s) 58, 253, 743, 963
HpaII CCGG 7 cut(s) 202, 286, 336, 481, 532, 735, 1065
HphI GGTGA 3 cut(s) 749, 1216, 1252
Hpy166II GTNNAC 3 cut(s) 387, 692, 932
Hpy188III TCNNGA 6 cut(s) 178, 250, 291, 481, 905, 967
Hpy8I GTNNAC 3 cut(s) 387, 692, 932
Hpy99I CGWCG 2 cut(s) 397, 671
HpyAV CCTTC 4 cut(s) 145, 671, 920, 1132
HpyCH4III ACNGT 5 cut(s) 21, 163, 599, 617, 831
HpyCH4IV ACGT 1 cut(s) 669
HpyCH4V TGCA 6 cut(s) 156, 367, 544, 780, 949, 1132
HpyF10VI GCNNNNNNNGC 8 cut(s) 76, 120, 150, 211, 220, 223, 556, 1078
HpyF3I CTNAG 2 cut(s) 104, 702
HpySE526I ACGT 1 cut(s) 669
Hsp92II CATG 1 cut(s) 949
Kpn2I TCCGGA 1 cut(s) 480
Ksp22I TGATCA 1 cut(s) 1086
KspI CCGCGG 1 cut(s) 221
Kzo9I GATC 7 cut(s) 100, 180, 524, 565, 954, 1086, 1420
LguI GCTCTTC 1 cut(s) 467
LmnI GCTCC 5 cut(s) 113, 282, 344, 547, 985
Lsp1109I GCAGC 2 cut(s) 140, 235
LweI GCATC 1 cut(s) 66
MaeI CTAG 5 cut(s) 444, 1073, 1098, 1424, 1432
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 2 cut(s) 10, 727
MalI GATC 7 cut(s) 102, 182, 526, 567, 956, 1088, 1422
MbiI CCGCTC 1 cut(s) 320
MboI GATC 7 cut(s) 100, 180, 524, 565, 954, 1086, 1420
MfeI CAATTG 1 cut(s) 657
MhlI GDGCHC 3 cut(s) 110, 415, 852
MluCI AATT 5 cut(s) 30, 657, 1340, 1393, 1409
MmeI TCCRAC 2 cut(s) 330, 1356
MroI TCCGGA 1 cut(s) 480
MroXI GAANNNNTTC 3 cut(s) 451, 476, 1409
MseI TTAA 2 cut(s) 348, 1302
MspA1I CMGCKG 2 cut(s) 123, 220
MspI CCGG 7 cut(s) 202, 286, 336, 481, 532, 735, 1065
MspR9I CCNGG 2 cut(s) 202, 533
MunI CAATTG 1 cut(s) 657
MvnI CGCG 2 cut(s) 220, 1267
MwoI GCNNNNNNNGC 8 cut(s) 76, 120, 150, 211, 220, 223, 556, 1078
NciI CCSGG 2 cut(s) 202, 533
NdeI CATATG 1 cut(s) 1134
NdeII GATC 7 cut(s) 100, 180, 524, 565, 954, 1086, 1420
NlaIII CATG 1 cut(s) 949
NlaIV GGNNCC 3 cut(s) 284, 340, 987
NmeAIII GCCGAG 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 176
PaqCI CACCTGC 1 cut(s) 785
PciSI GCTCTTC 1 cut(s) 467
PdmI GAANNNNTTC 3 cut(s) 451, 476, 1409
PfeI GAWTC 4 cut(s) 58, 253, 743, 963
PfoI TCCNGGA 1 cut(s) 531
PkrI GCNGC 8 cut(s) 125, 155, 216, 219, 222, 225, 425, 462
Psp124BI GAGCTC 1 cut(s) 110
PspN4I GGNNCC 3 cut(s) 284, 340, 987
PspPI GGNCC 1 cut(s) 288
PstI CTGCAG 1 cut(s) 546
RsaI GTAC 6 cut(s) 402, 432, 583, 592, 931, 1218
RsaNI GTAC 6 cut(s) 401, 431, 582, 591, 930, 1217
SacI GAGCTC 1 cut(s) 110
SacII CCGCGG 1 cut(s) 221
SapI GCTCTTC 1 cut(s) 467
SaqAI TTAA 2 cut(s) 348, 1302
SatI GCNGC 8 cut(s) 124, 154, 215, 218, 221, 224, 424, 461
Sau3AI GATC 7 cut(s) 100, 180, 524, 565, 954, 1086, 1420
Sau96I GGNCC 1 cut(s) 288
ScrFI CCNGG 2 cut(s) 202, 533
SduI GDGCHC 3 cut(s) 110, 415, 852
SfaNI GCATC 1 cut(s) 66
SfcI CTRYAG 1 cut(s) 542
Sfr274I CTCGAG 1 cut(s) 176
Sfr303I CCGCGG 1 cut(s) 221
SgrBI CCGCGG 1 cut(s) 221
SinI GGWCC 1 cut(s) 288
SlaI CTCGAG 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SpeI ACTAGT 1 cut(s) 1097
Sse9I AATT 5 cut(s) 30, 657, 1340, 1393, 1409
SspMI CTAG 5 cut(s) 444, 1073, 1098, 1424, 1432
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 2 cut(s) 200, 531
TaaI ACNGT 5 cut(s) 21, 163, 599, 617, 831
TaiI ACGT 1 cut(s) 672
TaqI TCGA 9 cut(s) 177, 183, 852, 919, 966, 993, 1115, 1182, 1278
TaqII GACCGA 1 cut(s) 1053
TasI AATT 5 cut(s) 30, 657, 1340, 1393, 1409
TatI WGTACW 2 cut(s) 400, 581
TauI GCSGC 6 cut(s) 126, 217, 220, 223, 426, 463
TfiI GAWTC 4 cut(s) 58, 253, 743, 963
Tru1I TTAA 2 cut(s) 348, 1302
Tru9I TTAA 2 cut(s) 348, 1302
TscAI CASTG 1 cut(s) 415
TseI GCWGC 2 cut(s) 153, 223
TspDTI ATGAA 5 cut(s) 885, 1151, 1352, 1362, 1422
TspGWI ACGGA 2 cut(s) 295, 331
TspRI CASTG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 288
XapI RAATTY 3 cut(s) 30, 1393, 1409
XhoI CTCGAG 1 cut(s) 176
XmnI GAANNNNTTC 3 cut(s) 451, 476, 1409
XspI CTAG 5 cut(s) 444, 1073, 1098, 1424, 1432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.