MD04G1092300.v1.1

Glycogen recognition site of AMP-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005701
Physical Location & Seq
Reverse (-)
16409989 .. 16415325
5337 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1092300.v1.1.491

Sequence Viewer

Length: 1833 bp
ATGGCGGCCACCGTCTCCCACCTCCCCGCCTTCCTCTCCGTCTTCTCTCAGAAGAATCTCTTCCTCGTCGACCCCCAACACCAATCCACTCTCAGATTCACCGCCCAGCAACGCCGCCCAGAGCCGCCGCACTTCACGCTTCAAGCTTCTTCCGTCAAGAAGAAAACCAGGAAGGTAAAGAGCAATGCCGAGCTCTGCAATGAACTCCGGCAGTTCCTCACTGCCGTCGGACTTCCGGAATGTCACGTTCCGTCCTTGAAGGAGCTCTCGCAGCGCGGAAGGGATGACCTTGCAAACATTGTCAGACGGAGAGGATATAAACTTATAAGAGAGCTTCTTGCGGACTCAAGCAAAAAAGATACAATTGGTAAGGCCGATAATGTGTTTGCTGGAATTCAGGATGCAATTGATGATCGTAAGAAGATAGTAACAGGTCAGGATCAAGAGGTGAATGATTCGGTTGAGGATTTCTCTTCGTCAATTGAAGTTTCAGTCTTGGAAACTAATTCTGGTAGTTTGATTTCTCATCCCGATCCGAGCCGTGATGACAGTGGTTGTTTAACTGTGGAATCTTCAGCTGATTTGTATAATTTAGAAGGTCATAGTGAGAAGGTGGATAATGTGAATGGAAGTGGTTCTGTGCCAACCAAATTTTCTGTAATGGAAAACCAATCCAGGAGTTCTGAAATTGGTCCACCCTTCAACTCTGACATTGATAGTAATACGCCTTCAGAAATTTCCACCCCTTCATCCTTGGGCGAACCTGATGATCTATCCTTGCCAACTACTGTTCACATCAAAGAAGCCAATACTTGTAGTAGTTCAAACGTTGGATTGGATCCGAACTCTGATGGATGTCCTGGCATGCCTGTAGAGCCTGCTGATGGTTTATCATTGGATGGAAAGGTCTTAGTTATATGTCAGGATGGGAAGGTTGACAATATGATTGATGAAGATTCCTCTTCAGCCGTAGTTTCCGGTTTGGAAGACCATTCTAGTTCAAATCTTGAATCAAGTCACAACTCTGATTACCATTACCCACCCTTAGAATCTCCACCCAATTTATCCTTAAAGGAAAAAGTGGCAAACTTTATGCAGAATGGAGACTTGGATGCAGTTGAAGATAATGTCTATGGCGTAGTAACTGAAAATAATGCTGAAGAAATCAAGGGTAGATTTGGAAACACTGAAGAAGTTCAATTAAGAACTCCTTCGGGGCACTCTAAAAATGTGCTTGAAGGAAGGGATGTAACTATGGCAGTTGATCCCTCTCTTAGGGATGATAGTTCACCAGCTGAAGGGCTAAGTTCTCTATATGACAAGGATTTGGATGTTAAGACAAATGAAAGAGACTATGAACTCGATGTTAGTCATCTCAAGTTTATGCTGCATCAGAAGGAGATCGAATTGTCACGGTTGAAAGAACAGATTAAGAAAGAACAGCTTGCTTTGTCAAACTTGCAAACCAAGGCTGAAATGGCAATCAGCAAAGCACAAAAGCTTGTCTCTGAAAAAGATGCAGAGTTACTTGCTGCTGAAGAAAGCCTTTCAGGATTAGTGGAGGTTGAGATCCAGTACGAGGGAGATGGCGAGATTGTGGAGGTGACTGGTAGCTTCAATGGTTGGCATCATCAGATTAAAATGGATCCAGAGTCATCATCTAGTATCATAGAACCCTTGAGGAAACCCAAACTTTGGTCAACAATGCTGTGGCTTTATCCAGGGACATATGAGATAAAATTCATTGTTGATGGCCAATGGAAGATTGATCCTCAAAGAGAGTCAGTTACCAGGGGTACCATATGTAACAACATTCTCCGCGTTGACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMPK1_CBM

Protein Analysis

611

Amino Acids

67.19

Weight (kDa)

4.79

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 521 - 608 1.4e-17 Glycogen recognition site of AMP-activated protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
Acc65I GGTACC 1 cut(s) 1796
AccB1I GGYRCC 1 cut(s) 1796
AccB7I CCANNNNNTGG 1 cut(s) 1695
AccI GTMKAC 1 cut(s) 69
AccII CGCG 2 cut(s) 276, 1821
AccIII TCCGGA 1 cut(s) 235
AciI CCGC 9 cut(s) 5, 27, 102, 115, 125, 128, 276, 341, 1819
AclI AACGTT 1 cut(s) 828
AclWI GGATC 9 cut(s) 447, 527, 833, 846, 1259, 1564, 1640, 1653, 1763
AcoI YGGCCR 2 cut(s) 6, 1753
AcsI RAATTY 4 cut(s) 393, 650, 735, 1739
AcuI CTGAAG 7 cut(s) 558, 714, 948, 1179, 1209, 1317, 1557
AfaI GTAC 2 cut(s) 1577, 1798
AfiI CCNNNNNNNGG 5 cut(s) 884, 1275, 1298, 1579, 1695
AjnI CCWGG 5 cut(s) 167, 674, 859, 1720, 1790
AjuI GAANNNNNNNTTGG 4 cut(s) 637, 669, 1091, 1123
AluBI AGCT 9 cut(s) 146, 193, 265, 334, 578, 1295, 1444, 1501, 1614
AluI AGCT 9 cut(s) 146, 193, 265, 334, 578, 1295, 1444, 1501, 1614
Alw21I GWGCWC 2 cut(s) 195, 267
Alw26I GTCTC 4 cut(s) 19, 1098, 1344, 1510
AlwI GGATC 9 cut(s) 447, 527, 833, 846, 1259, 1564, 1640, 1653, 1763
Aor13HI TCCGGA 1 cut(s) 235
AoxI GGCC 3 cut(s) 6, 372, 1753
ApeKI GCWGC 3 cut(s) 271, 1387, 1532
ApoI RAATTY 4 cut(s) 393, 650, 735, 1739
Asp700I GAANNNNTTC 4 cut(s) 59, 634, 1194, 1210
Asp718I GGTACC 1 cut(s) 1796
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 692
AsuHPI GGTGA 4 cut(s) 91, 460, 1281, 1615
AvaII GGWCC 1 cut(s) 692
BaeGI GKGCMC 1 cut(s) 1221
BalI TGGCCA 1 cut(s) 1755
BamHI GGATCC 2 cut(s) 838, 1645
BanI GGYRCC 1 cut(s) 1796
BanII GRGCYC 2 cut(s) 195, 267
BarI GAAGNNNNNNTAC 2 cut(s) 712, 744
BbsI GAAGAC 2 cut(s) 34, 993
Bbv12I GWGCWC 2 cut(s) 195, 267
BbvI GCAGC 3 cut(s) 283, 1374, 1519
BccI CCATC 6 cut(s) 845, 878, 893, 920, 1580, 1745
BceAI ACGGC 3 cut(s) 209, 525, 953
BciT130I CCWGG 5 cut(s) 169, 676, 861, 1722, 1792
BcoDI GTCTC 4 cut(s) 19, 1098, 1344, 1510
BfaI CTAG 2 cut(s) 996, 1662
BfmI CTRYAG 1 cut(s) 870
BisI GCNGC 7 cut(s) 6, 115, 125, 128, 272, 1388, 1533
BlsI GCNGC 7 cut(s) 7, 116, 126, 129, 273, 1389, 1534
Bme1390I CCNGG 5 cut(s) 169, 676, 861, 1722, 1792
Bme18I GGWCC 1 cut(s) 692
BmgT120I GGNCC 1 cut(s) 692
BmiI GGNNCC 3 cut(s) 840, 1647, 1798
BmrFI CCNGG 5 cut(s) 169, 676, 861, 1722, 1792
BmsI GCATC 5 cut(s) 391, 1102, 1399, 1507, 1636
BpiI GAAGAC 2 cut(s) 34, 993
BplI GAGNNNNNCTC 2 cut(s) 455, 487
BpuEI CTTGAG 3 cut(s) 331, 1361, 1699
BsaJI CCNNGG 4 cut(s) 753, 1467, 1721, 1791
BsaWI WCCGGW 2 cut(s) 235, 977
Bsc4I CCNNNNNNNGG 5 cut(s) 884, 1275, 1298, 1579, 1695
Bse1I ACTGG 2 cut(s) 1573, 1612
Bse3DI GCAATG 2 cut(s) 190, 205
BseAI TCCGGA 1 cut(s) 235
BseBI CCWGG 5 cut(s) 169, 676, 861, 1722, 1792
BseDI CCNNGG 4 cut(s) 753, 1467, 1721, 1791
BseLI CCNNNNNNNGG 5 cut(s) 884, 1275, 1298, 1579, 1695
BseMI GCAATG 2 cut(s) 190, 205
BseMII CTCAG 2 cut(s) 62, 106
BseNI ACTGG 2 cut(s) 1573, 1612
BseSI GKGCMC 1 cut(s) 1221
BseXI GCAGC 3 cut(s) 283, 1374, 1519
BseYI CCCAGC 1 cut(s) 105
Bsh1236I CGCG 2 cut(s) 276, 1821
BshFI GGCC 3 cut(s) 8, 374, 1755
BshNI GGYRCC 1 cut(s) 1796
BsiHKAI GWGCWC 2 cut(s) 195, 267
BsiSI CCGG 3 cut(s) 208, 236, 978
BslFI GGGAC 1 cut(s) 1738
BslI CCNNNNNNNGG 5 cut(s) 884, 1275, 1298, 1579, 1695
BsmAI GTCTC 4 cut(s) 19, 1098, 1344, 1510
BsmBI CGTCTC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 1738
BsnI GGCC 3 cut(s) 8, 374, 1755
Bsp1286I GDGCHC 3 cut(s) 195, 267, 1221
Bsp13I TCCGGA 1 cut(s) 235
BspACI CCGC 9 cut(s) 5, 27, 102, 115, 125, 128, 276, 341, 1819
BspANI GGCC 3 cut(s) 8, 374, 1755
BspCNI CTCAG 2 cut(s) 61, 105
BspEI TCCGGA 1 cut(s) 235
BspFNI CGCG 2 cut(s) 276, 1821
BspLI GGNNCC 3 cut(s) 840, 1647, 1798
BspPI GGATC 9 cut(s) 447, 527, 833, 846, 1259, 1564, 1640, 1653, 1763
BspT107I GGYRCC 1 cut(s) 1796
BsrDI GCAATG 2 cut(s) 190, 205
BsrI ACTGG 2 cut(s) 1573, 1612
BssECI CCNNGG 4 cut(s) 753, 1467, 1721, 1791
BssT1I CCWWGG 2 cut(s) 753, 1467
Bst2UI CCWGG 5 cut(s) 169, 676, 861, 1722, 1792
Bst4CI ACNGT 5 cut(s) 13, 551, 565, 790, 1416
Bst6I CTCTTC 3 cut(s) 65, 478, 967
BstC8I GCNNGC 3 cut(s) 866, 879, 1446
BstDEI CTNAG 6 cut(s) 48, 92, 910, 1045, 1274, 1304
BstENI CCTNNNNNAGG 1 cut(s) 1273
BstFNI CGCG 2 cut(s) 276, 1821
BstHHI GCGC 1 cut(s) 276
BstMAI GTCTC 4 cut(s) 19, 1098, 1344, 1510
BstMWI GCNNNNNNNGC 4 cut(s) 136, 271, 874, 1478
BstNI CCWGG 5 cut(s) 169, 676, 861, 1722, 1792
BstNSI RCATGY 1 cut(s) 868
BstSCI CCNGG 5 cut(s) 167, 674, 859, 1720, 1790
BstSFI CTRYAG 1 cut(s) 870
BstSLI GKGCMC 1 cut(s) 1221
BstUI CGCG 2 cut(s) 276, 1821
BstV1I GCAGC 3 cut(s) 283, 1374, 1519
BstV2I GAAGAC 2 cut(s) 34, 993
BstX2I RGATCY 3 cut(s) 838, 1569, 1645
BstYI RGATCY 3 cut(s) 838, 1569, 1645
BsuRI GGCC 3 cut(s) 8, 374, 1755
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 3 cut(s) 219, 556, 1185
Cac8I GCNNGC 3 cut(s) 866, 879, 1446
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 692
Csp6I GTAC 2 cut(s) 1576, 1797
CviAII CATG 1 cut(s) 865
CviQI GTAC 2 cut(s) 1576, 1797
DdeI CTNAG 6 cut(s) 48, 92, 910, 1045, 1274, 1304
EaeI YGGCCR 2 cut(s) 6, 1753
Eam1104I CTCTTC 3 cut(s) 65, 478, 967
EarI CTCTTC 3 cut(s) 65, 478, 967
Ecl136II GAGCTC 2 cut(s) 193, 265
Eco130I CCWWGG 2 cut(s) 753, 1467
Eco24I GRGCYC 2 cut(s) 195, 267
Eco47I GGWCC 1 cut(s) 692
Eco53kI GAGCTC 2 cut(s) 193, 265
Eco57I CTGAAG 7 cut(s) 558, 714, 948, 1179, 1209, 1317, 1557
EcoICRI GAGCTC 2 cut(s) 193, 265
EcoNI CCTNNNNNAGG 1 cut(s) 1273
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 5 cut(s) 167, 674, 859, 1720, 1790
EcoT14I CCWWGG 2 cut(s) 753, 1467
EcoT38I GRGCYC 2 cut(s) 195, 267
ErhI CCWWGG 2 cut(s) 753, 1467
Esp3I CGTCTC 1 cut(s) 19
FaeI CATG 1 cut(s) 868
FalI AAGNNNNNCTT 8 cut(s) 44, 76, 1195, 1227, 1428, 1460, 1530, 1562
FaqI GGGAC 1 cut(s) 1738
FatI CATG 1 cut(s) 864
FauI CCCGC 1 cut(s) 34
FauNDI CATATG 2 cut(s) 1729, 1802
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 7 cut(s) 6, 115, 125, 128, 272, 1388, 1533
FriOI GRGCYC 2 cut(s) 195, 267
Fsp4HI GCNGC 7 cut(s) 6, 115, 125, 128, 272, 1388, 1533
FspBI CTAG 2 cut(s) 996, 1662
GlaI GCGC 1 cut(s) 275
GluI GCNGC 7 cut(s) 6, 115, 125, 128, 272, 1388, 1533
GsaI CCCAGC 1 cut(s) 109
HaeIII GGCC 3 cut(s) 8, 374, 1755
HapII CCGG 3 cut(s) 208, 236, 978
HhaI GCGC 1 cut(s) 276
Hin1II CATG 1 cut(s) 868
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 4 cut(s) 70, 937, 1701, 1825
HindII GTYRAC 4 cut(s) 70, 937, 1701, 1825
HindIII AAGCTT 2 cut(s) 144, 1499
HpaII CCGG 3 cut(s) 208, 236, 978
HphI GGTGA 4 cut(s) 91, 460, 1281, 1615
Hpy166II GTNNAC 7 cut(s) 70, 695, 793, 937, 1289, 1701, 1825
Hpy8I GTNNAC 7 cut(s) 70, 695, 793, 937, 1289, 1701, 1825
Hpy99I CGWCG 2 cut(s) 71, 230
HpyCH4III ACNGT 5 cut(s) 13, 551, 565, 790, 1416
HpyCH4IV ACGT 2 cut(s) 246, 828
HpyCH4V TGCA 8 cut(s) 198, 293, 404, 1096, 1115, 1390, 1462, 1520
HpyF10VI GCNNNNNNNGC 4 cut(s) 136, 271, 874, 1478
HpyF3I CTNAG 6 cut(s) 48, 92, 910, 1045, 1274, 1304
HpySE526I ACGT 2 cut(s) 246, 828
Hsp92II CATG 1 cut(s) 868
HspAI GCGC 1 cut(s) 274
Kpn2I TCCGGA 1 cut(s) 235
KpnI GGTACC 1 cut(s) 1800
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 3 cut(s) 283, 1374, 1519
LweI GCATC 5 cut(s) 391, 1102, 1399, 1507, 1636
MaeI CTAG 2 cut(s) 996, 1662
MaeII ACGT 2 cut(s) 246, 828
MfeI CAATTG 3 cut(s) 363, 405, 480
MflI RGATCY 3 cut(s) 838, 1569, 1645
MhlI GDGCHC 3 cut(s) 195, 267, 1221
MlsI TGGCCA 1 cut(s) 1755
MluNI TGGCCA 1 cut(s) 1755
MlyI GAGTC 3 cut(s) 338, 1661, 1790
MmeI TCCRAC 2 cut(s) 208, 811
Mox20I TGGCCA 1 cut(s) 1755
MroI TCCGGA 1 cut(s) 235
MroXI GAANNNNTTC 4 cut(s) 59, 634, 1194, 1210
MscI TGGCCA 1 cut(s) 1755
MseI TTAA 6 cut(s) 560, 1070, 1202, 1335, 1431, 1638
Msp20I TGGCCA 1 cut(s) 1755
MspA1I CMGCKG 2 cut(s) 578, 1295
MspI CCGG 3 cut(s) 208, 236, 978
MspR9I CCNGG 5 cut(s) 169, 676, 861, 1722, 1792
MunI CAATTG 3 cut(s) 363, 405, 480
MvaI CCWGG 5 cut(s) 169, 676, 861, 1722, 1792
MvnI CGCG 2 cut(s) 276, 1821
MwoI GCNNNNNNNGC 4 cut(s) 136, 271, 874, 1478
NdeI CATATG 2 cut(s) 1729, 1802
NlaIII CATG 1 cut(s) 868
NlaIV GGNNCC 3 cut(s) 840, 1647, 1798
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 4 cut(s) 242, 1016, 1410, 1603
NspI RCATGY 1 cut(s) 868
PaeI GCATGC 1 cut(s) 868
PdmI GAANNNNTTC 4 cut(s) 59, 634, 1194, 1210
PfeI GAWTC 7 cut(s) 55, 96, 455, 569, 956, 1010, 1049
PflMI CCANNNNNTGG 1 cut(s) 1695
PfoI TCCNGGA 1 cut(s) 674
PkrI GCNGC 7 cut(s) 7, 116, 126, 129, 273, 1389, 1534
PleI GAGTC 3 cut(s) 338, 1660, 1789
PpsI GAGTC 3 cut(s) 338, 1660, 1789
PsiI TTATAA 1 cut(s) 326
Psp124BI GAGCTC 2 cut(s) 195, 267
Psp1406I AACGTT 1 cut(s) 828
Psp6I CCWGG 5 cut(s) 167, 674, 859, 1720, 1790
PspFI CCCAGC 1 cut(s) 105
PspGI CCWGG 5 cut(s) 167, 674, 859, 1720, 1790
PspN4I GGNNCC 3 cut(s) 840, 1647, 1798
PspPI GGNCC 1 cut(s) 692
PsuI RGATCY 3 cut(s) 838, 1569, 1645
PvuII CAGCTG 2 cut(s) 578, 1295
RsaI GTAC 2 cut(s) 1577, 1798
RsaNI GTAC 2 cut(s) 1576, 1797
SacI GAGCTC 2 cut(s) 195, 267
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 6 cut(s) 560, 1070, 1202, 1335, 1431, 1638
SatI GCNGC 7 cut(s) 6, 115, 125, 128, 272, 1388, 1533
Sau96I GGNCC 1 cut(s) 692
SchI GAGTC 3 cut(s) 338, 1661, 1790
ScrFI CCNGG 5 cut(s) 169, 676, 861, 1722, 1792
SduI GDGCHC 3 cut(s) 195, 267, 1221
SfaNI GCATC 5 cut(s) 391, 1102, 1399, 1507, 1636
SfcI CTRYAG 1 cut(s) 870
SinI GGWCC 1 cut(s) 692
SmlI CTYRAG 3 cut(s) 346, 1376, 1678
SmoI CTYRAG 3 cut(s) 346, 1376, 1678
SphI GCATGC 1 cut(s) 868
SsiI CCGC 9 cut(s) 5, 27, 102, 115, 125, 128, 276, 341, 1819
SspMI CTAG 2 cut(s) 996, 1662
SstI GAGCTC 2 cut(s) 195, 267
StyD4I CCNGG 5 cut(s) 167, 674, 859, 1720, 1790
StyI CCWWGG 2 cut(s) 753, 1467
TaaI ACNGT 5 cut(s) 13, 551, 565, 790, 1416
TaiI ACGT 2 cut(s) 249, 831
TaqI TCGA 3 cut(s) 69, 1362, 1404
TauI GCSGC 4 cut(s) 8, 117, 127, 130
TfiI GAWTC 7 cut(s) 55, 96, 455, 569, 956, 1010, 1049
Tru1I TTAA 6 cut(s) 560, 1070, 1202, 1335, 1431, 1638
Tru9I TTAA 6 cut(s) 560, 1070, 1202, 1335, 1431, 1638
TscAI CASTG 3 cut(s) 226, 556, 1192
TseFI GTSAC 4 cut(s) 242, 1016, 1410, 1603
TseI GCWGC 3 cut(s) 271, 1387, 1532
Tsp45I GTSAC 4 cut(s) 242, 1016, 1410, 1603
TspDTI ATGAA 6 cut(s) 216, 738, 966, 1359, 1371, 1732
TspGWI ACGGA 4 cut(s) 28, 142, 240, 322
TspRI CASTG 3 cut(s) 226, 556, 1192
Van91I CCANNNNNTGG 1 cut(s) 1695
VpaK11BI GGWCC 1 cut(s) 692
XagI CCTNNNNNAGG 1 cut(s) 1273
XapI RAATTY 4 cut(s) 393, 650, 735, 1739
XceI RCATGY 1 cut(s) 868
XcmI CCANNNNNNNNNTGG 1 cut(s) 1474
XmiI GTMKAC 1 cut(s) 69
XmnI GAANNNNTTC 4 cut(s) 59, 634, 1194, 1210
XspI CTAG 2 cut(s) 996, 1662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.