MD12G1110100.v1.1

Glycogen recognition site of AMP-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005701
Physical Location & Seq
Reverse (-)
17408487 .. 17413159
4673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1110100.v1.1.491

Sequence Viewer

Length: 1845 bp
ATGGCGGCCCCCGTCTCTCACCTCCCCGCCTTCCTCTCCCTCTTCTCTCACAAGCTCTTCTTCGCCCACCCGCACCAGCAATCCACTCTCGGATTCACCGCCCTACAACGCTGTCCACCGCCGCCGCGCTGCACCCTTCGCGCTTCTTCCGTCAGGAAGAAAACTAGGAAGGTAAAGAGCAATGCGGAGCTCTGCAATGATCTCCGGCAGTTCCTCACTGCGGTCGGACTTTCGGAATGTCACGTTCCATCCTTGAAGGAGCTCTCGCAGCACGGAAGGAATGATCTTGCAAACATCGTCAGACGAAGAGGATATAAACTTATAAGAGAGCTTCTTGTGGACTCGAGCAATACAGATACCAATGGTAATGCCGATACCGGATTTGCTGGAATTCAGGATGCAATCAATGATCGTAAAGAGATAGTAACAGGTCAGGATCAAGAGGTGAATGATGTGATTGAGGATTTTTCTTCGTCAACTGAAGTTTCAGACTTGGAAAGTAATTCTGGTAGTTTGATTTCTCCTCCCGATCTGAGCCTCAATGACAGTGATATTACCGTGGAGTCTTCAGCTGATTTGTATAATTTAGACGGTCATAGTGAGCAGGTGGATGTGAATGGAAATGGTTCTGTGTCAACCGAATTTTCTGTTACGGAAAACCAATCCAGGAGTTCTAAAATTTGTCCACACTTCAACTCTGACATTGGTACTAATATGCCTTCAGAAATTTTCACCCCTTCATCCTTCGGCGAACTTGATGACCTATCCTTGTCAACCACTTATCATATCAAAGAAACCAATTCTTCCAGTTCAAATGTTGGTTTGGATCTGAACTCCGATGGACGTCCTTGCATGCCTGTAGAATCTGTTGATGGTTTGTCATTGGATGAAAAGGTCTTAGATATATGTCAGGATGGGAAGGTTGACAATATGGCTAATGAAGATTGCTCTTCAACCACAGTTTCCGTTTTGGAAGACCATTCTAGTTCAGATCTTGATCCAAGTCACAACTCTGATCATCATAGTTATCCGCCTTTAGAATCTCCACCCAATTTATCCTTGAAGGAAAAAGTGGAAAACTTTATGCAGAATGGAGACTTGGATGCAGTTGAAGATAATGTCTATGGCATATTAACTGAGAATAATGATGAAGAAATCAAGGAAGAATATAGATTTGGAAACACTGAGGAGGTTCAAATAAGAATTCCTAAAAATGTGCCTGACAGAAGTGATGCAATTATGGCATTGAATCAAAGGACATCAACATCAACTACGGCAGTTGATCCCTCTCTTAGGTTGGATAGTTCATCAACTGAAGGGCTGAGTTCTCTATATGACAAGGATTTGGATGATAAGACAAGTGAAAGTGAGGATCGACTTGAACTTAATCATCTCAAGTTCATGCTGCATCAGAAGGAGATGGAATTGTCACGGTTGAAAGAACAGATTGAGAAGGAAGAGCTTTTTTTGTCAAACTTGCAAACCAAGTCTGAAATGGCAATGAGCAAAGCACAAAAGCTTGTCTCTGAAAAAGATGCAGAGTTACTTGCTGATGAAGAAAGCCTTTCAGGATTAGTGGAGGTTGAGATTCAGTACCAGGGAGATGGCGAGATTGTGGAGGTGACTGGTAGCTTCAATGGTTGGTATCATCAGATTGAAATGGATTCACAGCCATCATCTAGTATCATAGAGAGGGAACCTAGACTATGGTCAACAATGCTGTGGCTTTATCCAGGGACGTATGAGATAAAATTCATTGTTGATGGCCAATGGATGATTGATCCTCAAAGAGAGTCAGTAACCAGGGGTACCATATGTAACAACATTCTCCATGTCAACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMPK1_CBM

Protein Analysis

615

Amino Acids

68.34

Weight (kDa)

4.55

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 527 - 613 7.3e-17 Glycogen recognition site of AMP-activated protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 323
AarI CACCTGC 1 cut(s) 595
AatII GACGTC 1 cut(s) 847
Acc36I ACCTGC 1 cut(s) 595
Acc65I GGTACC 1 cut(s) 1808
AccB1I GGYRCC 1 cut(s) 1808
AccII CGCG 2 cut(s) 127, 141
AclWI GGATC 6 cut(s) 444, 834, 992, 1277, 1380, 1775
AcoI YGGCCR 1 cut(s) 1765
AcsI RAATTY 6 cut(s) 390, 641, 678, 726, 1203, 1751
AcuI CTGAAG 4 cut(s) 501, 552, 705, 1335
AcyI GRCGYC 1 cut(s) 844
AfaI GTAC 3 cut(s) 709, 1595, 1810
AfiI CCNNNNNNNGG 2 cut(s) 220, 1293
AjnI CCWGG 4 cut(s) 665, 1596, 1732, 1802
AjuI GAANNNNNNNTTGG 4 cut(s) 1082, 1114, 1158, 1190
AluBI AGCT 8 cut(s) 55, 190, 262, 331, 572, 1462, 1519, 1632
AluI AGCT 8 cut(s) 55, 190, 262, 331, 572, 1462, 1519, 1632
Alw21I GWGCWC 2 cut(s) 192, 264
Alw26I GTCTC 3 cut(s) 19, 1089, 1528
AlwI GGATC 6 cut(s) 444, 834, 992, 1277, 1380, 1775
Ama87I CYCGRG 1 cut(s) 343
AoxI GGCC 2 cut(s) 6, 1765
ApeKI GCWGC 3 cut(s) 129, 268, 1405
ApoI RAATTY 6 cut(s) 390, 641, 678, 726, 1203, 1751
Asp700I GAANNNNTTC 1 cut(s) 625
Asp718I GGTACC 1 cut(s) 1808
AspLEI GCGC 2 cut(s) 129, 143
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 5 cut(s) 11, 88, 457, 724, 1633
AvaI CYCGRG 1 cut(s) 343
BalI TGGCCA 1 cut(s) 1767
BanI GGYRCC 1 cut(s) 1808
BanII GRGCYC 2 cut(s) 192, 264
BbsI GAAGAC 2 cut(s) 558, 981
Bbv12I GWGCWC 2 cut(s) 192, 264
BbvI GCAGC 3 cut(s) 116, 280, 1392
BccI CCATC 8 cut(s) 256, 833, 866, 908, 1414, 1598, 1681, 1757
BceAI ACGGC 1 cut(s) 1290
BciT130I CCWGG 4 cut(s) 667, 1598, 1734, 1804
BclI TGATCA 1 cut(s) 1015
BcoDI GTCTC 3 cut(s) 19, 1089, 1528
BfaI CTAG 4 cut(s) 165, 984, 1680, 1701
BfmI CTRYAG 1 cut(s) 858
BfuAI ACCTGC 1 cut(s) 595
BglII AGATCT 1 cut(s) 991
BisI GCNGC 6 cut(s) 6, 122, 125, 130, 269, 1406
BlsI GCNGC 6 cut(s) 7, 123, 126, 131, 270, 1407
Bme1390I CCNGG 4 cut(s) 667, 1598, 1734, 1804
BmeT110I CYCGRG 1 cut(s) 343
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 3 cut(s) 9, 1698, 1810
BmrFI CCNGG 4 cut(s) 667, 1598, 1734, 1804
BmsI GCATC 5 cut(s) 388, 1093, 1222, 1417, 1525
BoxI GACNNNNGTC 1 cut(s) 1708
BpiI GAAGAC 2 cut(s) 558, 981
BpuEI CTTGAG 1 cut(s) 1379
BsaBI GATNNNNATC 1 cut(s) 996
BsaHI GRCGYC 1 cut(s) 844
BsaJI CCNNGG 4 cut(s) 558, 1597, 1733, 1803
BsaWI WCCGGW 1 cut(s) 377
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 1293
Bse1I ACTGG 2 cut(s) 807, 1630
Bse3DI GCAATG 3 cut(s) 187, 202, 1506
Bse8I GATNNNNATC 1 cut(s) 996
BseBI CCWGG 4 cut(s) 667, 1598, 1734, 1804
BseDI CCNNGG 4 cut(s) 558, 1597, 1733, 1803
BseGI GGATG 9 cut(s) 248, 403, 616, 740, 892, 919, 1108, 1354, 1779
BseJI GATNNNNATC 1 cut(s) 996
BseLI CCNNNNNNNGG 2 cut(s) 220, 1293
BseMI GCAATG 3 cut(s) 187, 202, 1506
BseMII CTCAG 4 cut(s) 524, 1128, 1176, 1313
BseNI ACTGG 2 cut(s) 807, 1630
BseRI GAGGAG 2 cut(s) 513, 1202
BseXI GCAGC 3 cut(s) 116, 280, 1392
BsgI GTGCAG 1 cut(s) 115
Bsh1236I CGCG 2 cut(s) 127, 141
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 2 cut(s) 8, 1767
BshNI GGYRCC 1 cut(s) 1808
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 2 cut(s) 192, 264
BsiHKCI CYCGRG 1 cut(s) 343
BsiSI CCGG 2 cut(s) 205, 378
BslFI GGGAC 1 cut(s) 1750
BslI CCNNNNNNNGG 2 cut(s) 220, 1293
BsmAI GTCTC 3 cut(s) 19, 1089, 1528
BsmBI CGTCTC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 1750
BsnI GGCC 2 cut(s) 8, 1767
BsoBI CYCGRG 1 cut(s) 343
Bsp1286I GDGCHC 2 cut(s) 192, 264
BspANI GGCC 2 cut(s) 8, 1767
BspCNI CTCAG 4 cut(s) 525, 1129, 1177, 1314
BspFNI CGCG 2 cut(s) 127, 141
BspLI GGNNCC 3 cut(s) 9, 1698, 1810
BspMI ACCTGC 1 cut(s) 595
BspPI GGATC 6 cut(s) 444, 834, 992, 1277, 1380, 1775
BspQI GCTCTTC 3 cut(s) 62, 955, 1452
BspT107I GGYRCC 1 cut(s) 1808
BsrDI GCAATG 3 cut(s) 187, 202, 1506
BsrI ACTGG 2 cut(s) 807, 1630
BssECI CCNNGG 4 cut(s) 558, 1597, 1733, 1803
BssNI GRCGYC 1 cut(s) 844
Bst2UI CCWGG 4 cut(s) 667, 1598, 1734, 1804
Bst4CI ACNGT 5 cut(s) 548, 559, 593, 961, 1434
Bst6I CTCTTC 5 cut(s) 47, 62, 301, 955, 1452
BstACI GRCGYC 1 cut(s) 844
BstC8I GCNNGC 1 cut(s) 854
BstDEI CTNAG 6 cut(s) 533, 898, 1137, 1185, 1292, 1322
BstDSI CCRYGG 1 cut(s) 558
BstENI CCTNNNNNAGG 1 cut(s) 1291
BstF5I GGATG 9 cut(s) 248, 403, 616, 740, 892, 919, 1108, 1354, 1779
BstFNI CGCG 2 cut(s) 127, 141
BstHHI GCGC 2 cut(s) 129, 143
BstMAI GTCTC 3 cut(s) 19, 1089, 1528
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 3 cut(s) 138, 268, 1241
BstNI CCWGG 4 cut(s) 667, 1598, 1734, 1804
BstNSI RCATGY 1 cut(s) 856
BstPAI GACNNNNGTC 1 cut(s) 1708
BstSCI CCNGG 4 cut(s) 665, 1596, 1732, 1802
BstSFI CTRYAG 1 cut(s) 858
BstUI CGCG 2 cut(s) 127, 141
BstV1I GCAGC 3 cut(s) 116, 280, 1392
BstV2I GAAGAC 2 cut(s) 558, 981
BstX2I RGATCY 2 cut(s) 826, 991
BstXI CCANNNNNNTGG 1 cut(s) 1604
BstYI RGATCY 2 cut(s) 826, 991
BsuRI GGCC 2 cut(s) 8, 1767
BtgI CCRYGG 1 cut(s) 558
BtsCI GGATG 9 cut(s) 248, 403, 616, 740, 892, 919, 1108, 1354, 1779
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 3 cut(s) 216, 553, 1182
BveI ACCTGC 1 cut(s) 595
Cac8I GCNNGC 1 cut(s) 854
CfoI GCGC 2 cut(s) 129, 143
Cfr13I GGNCC 1 cut(s) 7
Csp6I GTAC 3 cut(s) 708, 1594, 1809
CviAII CATG 3 cut(s) 853, 1402, 1832
CviQI GTAC 3 cut(s) 708, 1594, 1809
DdeI CTNAG 6 cut(s) 533, 898, 1137, 1185, 1292, 1322
EaeI YGGCCR 1 cut(s) 1765
Eam1104I CTCTTC 5 cut(s) 47, 62, 301, 955, 1452
EarI CTCTTC 5 cut(s) 47, 62, 301, 955, 1452
EciI GGCGGA 1 cut(s) 1020
Ecl136II GAGCTC 2 cut(s) 190, 262
Eco24I GRGCYC 2 cut(s) 192, 264
Eco53kI GAGCTC 2 cut(s) 190, 262
Eco57I CTGAAG 4 cut(s) 501, 552, 705, 1335
Eco88I CYCGRG 1 cut(s) 343
EcoICRI GAGCTC 2 cut(s) 190, 262
EcoNI CCTNNNNNAGG 1 cut(s) 1291
EcoRI GAATTC 2 cut(s) 390, 1203
EcoRII CCWGG 4 cut(s) 665, 1596, 1732, 1802
EcoT38I GRGCYC 2 cut(s) 192, 264
Esp3I CGTCTC 1 cut(s) 19
FaeI CATG 3 cut(s) 856, 1405, 1835
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 1548, 1580
FaqI GGGAC 1 cut(s) 1750
FatI CATG 3 cut(s) 852, 1401, 1831
FauI CCCGC 2 cut(s) 34, 78
FauNDI CATATG 1 cut(s) 1814
FbaI TGATCA 1 cut(s) 1015
Fnu4HI GCNGC 6 cut(s) 6, 122, 125, 130, 269, 1406
FokI GGATG 9 cut(s) 235, 410, 623, 727, 899, 926, 1115, 1361, 1786
FriOI GRGCYC 2 cut(s) 192, 264
Fsp4HI GCNGC 6 cut(s) 6, 122, 125, 130, 269, 1406
FspBI CTAG 4 cut(s) 165, 984, 1680, 1701
GlaI GCGC 2 cut(s) 128, 142
GluI GCNGC 6 cut(s) 6, 122, 125, 130, 269, 1406
HaeIII GGCC 2 cut(s) 8, 1767
HapII CCGG 2 cut(s) 205, 378
HhaI GCGC 2 cut(s) 129, 143
Hin1I GRCGYC 1 cut(s) 844
Hin1II CATG 3 cut(s) 856, 1405, 1835
Hin6I GCGC 2 cut(s) 127, 141
HinP1I GCGC 2 cut(s) 127, 141
HincII GTYRAC 6 cut(s) 477, 636, 774, 925, 1713, 1837
HindII GTYRAC 6 cut(s) 477, 636, 774, 925, 1713, 1837
HindIII AAGCTT 1 cut(s) 1517
HinfI GANTC 9 cut(s) 93, 341, 563, 863, 1040, 1249, 1588, 1664, 1793
HpaII CCGG 2 cut(s) 205, 378
HphI GGTGA 5 cut(s) 11, 88, 457, 724, 1633
Hpy166II GTNNAC 9 cut(s) 116, 340, 477, 636, 686, 774, 925, 1713, 1837
Hpy188III TCNNGA 8 cut(s) 154, 395, 434, 440, 527, 911, 995, 1569
Hpy8I GTNNAC 9 cut(s) 116, 340, 477, 636, 686, 774, 925, 1713, 1837
HpyCH4III ACNGT 5 cut(s) 548, 559, 593, 961, 1434
HpyCH4IV ACGT 3 cut(s) 243, 844, 1739
HpyF10VI GCNNNNNNNGC 3 cut(s) 138, 268, 1241
HpyF3I CTNAG 6 cut(s) 533, 898, 1137, 1185, 1292, 1322
HpySE526I ACGT 3 cut(s) 243, 844, 1739
Hsp92I GRCGYC 1 cut(s) 844
Hsp92II CATG 3 cut(s) 856, 1405, 1835
HspAI GCGC 2 cut(s) 127, 141
KpnI GGTACC 1 cut(s) 1812
Ksp22I TGATCA 1 cut(s) 1015
LguI GCTCTTC 3 cut(s) 62, 955, 1452
LmnI GCTCC 2 cut(s) 187, 259
Lsp1109I GCAGC 3 cut(s) 116, 280, 1392
LweI GCATC 5 cut(s) 388, 1093, 1222, 1417, 1525
MaeI CTAG 4 cut(s) 165, 984, 1680, 1701
MaeII ACGT 3 cut(s) 243, 844, 1739
MaeIII GTNAC 9 cut(s) 239, 424, 649, 1004, 1428, 1542, 1621, 1798, 1817
MflI RGATCY 2 cut(s) 826, 991
MhlI GDGCHC 2 cut(s) 192, 264
MlsI TGGCCA 1 cut(s) 1767
MluNI TGGCCA 1 cut(s) 1767
MlyI GAGTC 3 cut(s) 335, 572, 1802
MmeI TCCRAC 2 cut(s) 205, 1278
Mox20I TGGCCA 1 cut(s) 1767
MroXI GAANNNNTTC 1 cut(s) 625
MscI TGGCCA 1 cut(s) 1767
MseI TTAA 2 cut(s) 1133, 1386
Msp20I TGGCCA 1 cut(s) 1767
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 2 cut(s) 205, 378
MspR9I CCNGG 4 cut(s) 667, 1598, 1734, 1804
MvaI CCWGG 4 cut(s) 667, 1598, 1734, 1804
MvnI CGCG 2 cut(s) 127, 141
MwoI GCNNNNNNNGC 3 cut(s) 138, 268, 1241
NdeI CATATG 1 cut(s) 1814
NlaIII CATG 3 cut(s) 856, 1405, 1835
NlaIV GGNNCC 3 cut(s) 9, 1698, 1810
NmuCI GTSAC 4 cut(s) 239, 1004, 1428, 1621
NspI RCATGY 1 cut(s) 856
PaeI GCATGC 1 cut(s) 856
PaeR7I CTCGAG 1 cut(s) 343
PaqCI CACCTGC 1 cut(s) 595
PciSI GCTCTTC 3 cut(s) 62, 955, 1452
PdmI GAANNNNTTC 1 cut(s) 625
PfeI GAWTC 6 cut(s) 93, 863, 1040, 1249, 1588, 1664
PfoI TCCNGGA 1 cut(s) 665
PkrI GCNGC 6 cut(s) 7, 123, 126, 131, 270, 1407
PleI GAGTC 3 cut(s) 335, 571, 1801
PpsI GAGTC 3 cut(s) 335, 571, 1801
PshAI GACNNNNGTC 1 cut(s) 1708
PsiI TTATAA 1 cut(s) 323
Psp124BI GAGCTC 2 cut(s) 192, 264
Psp6I CCWGG 4 cut(s) 665, 1596, 1732, 1802
PspGI CCWGG 4 cut(s) 665, 1596, 1732, 1802
PspN4I GGNNCC 3 cut(s) 9, 1698, 1810
PspPI GGNCC 1 cut(s) 7
PspXI VCTCGAGB 1 cut(s) 343
PsuI RGATCY 2 cut(s) 826, 991
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 3 cut(s) 709, 1595, 1810
RsaNI GTAC 3 cut(s) 708, 1594, 1809
SacI GAGCTC 2 cut(s) 192, 264
SapI GCTCTTC 3 cut(s) 62, 955, 1452
SaqAI TTAA 2 cut(s) 1133, 1386
SatI GCNGC 6 cut(s) 6, 122, 125, 130, 269, 1406
Sau96I GGNCC 1 cut(s) 7
SchI GAGTC 3 cut(s) 335, 572, 1802
ScrFI CCNGG 4 cut(s) 667, 1598, 1734, 1804
SduI GDGCHC 2 cut(s) 192, 264
SfaNI GCATC 5 cut(s) 388, 1093, 1222, 1417, 1525
SfcI CTRYAG 1 cut(s) 858
Sfr274I CTCGAG 1 cut(s) 343
SlaI CTCGAG 1 cut(s) 343
SmlI CTYRAG 2 cut(s) 343, 1394
SmoI CTYRAG 2 cut(s) 343, 1394
SphI GCATGC 1 cut(s) 856
SspMI CTAG 4 cut(s) 165, 984, 1680, 1701
SstI GAGCTC 2 cut(s) 192, 264
StyD4I CCNGG 4 cut(s) 665, 1596, 1732, 1802
TaaI ACNGT 5 cut(s) 548, 559, 593, 961, 1434
TaiI ACGT 3 cut(s) 246, 847, 1742
TaqI TCGA 2 cut(s) 344, 1375
TauI GCSGC 3 cut(s) 8, 124, 127
TfiI GAWTC 6 cut(s) 93, 863, 1040, 1249, 1588, 1664
Tru1I TTAA 2 cut(s) 1133, 1386
Tru9I TTAA 2 cut(s) 1133, 1386
TscAI CASTG 3 cut(s) 223, 553, 1189
TseFI GTSAC 4 cut(s) 239, 1004, 1428, 1621
TseI GCWGC 3 cut(s) 129, 268, 1405
Tsp45I GTSAC 4 cut(s) 239, 1004, 1428, 1621
TspDTI ATGAA 8 cut(s) 729, 903, 954, 1164, 1296, 1390, 1569, 1744
TspGWI ACGGA 4 cut(s) 139, 288, 668, 955
TspRI CASTG 3 cut(s) 223, 553, 1189
XagI CCTNNNNNAGG 1 cut(s) 1291
XapI RAATTY 6 cut(s) 390, 641, 678, 726, 1203, 1751
XceI RCATGY 1 cut(s) 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 1492
XhoI CTCGAG 1 cut(s) 343
XmnI GAANNNNTTC 1 cut(s) 625
XspI CTAG 4 cut(s) 165, 984, 1680, 1701
ZraI GACGTC 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.