MD04G1102200.v1.1

Cell number regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009470
Physical Location & Seq
Reverse (-)
18903406 .. 18906014
2609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1102200.v1.1.491

Sequence Viewer

Length: 741 bp
ATGGCGACCAACAACAGGGAGAGCAATGTCCAGGAGGAATCGAGCCCTCTTCTGAGCAAGCAATTCGAGGAGAACCAGAAGGATGCTGAGAAGCCCGCCAAGGCCTCGCCGGAGAAATCGCCGACGGATGTGGGGCATGTGAATGGAGGTGGTAATGGGTGCGTGTGGACCGCCGATGGGCTGCCCTTGGGGCACGGGAGCGTGATGGGTGAGCCTATGGGTCGGGCTCAGTGGGACTCCAGCATCCTTGCTTGTCTTGGGCGTAATGATGAGTTCTGTAGCAGCGATCTCGAAGTTTGTCTTCTTGGAAGTGTGGCTCCTTGTGTGTTGTATGGAAGCAATGCTGAGAGACTTGGATCTGCTCCTGGGACGTTTGCAAATCACTGTTTGCCATACTCTGCTCTTTATCTGATTGGGAACGCCTTTTTTGGTTGGAACTGCCTTGCACCGTGGTTTTCATATCCTAGGCGTACTGCCATCCGCCGCAAATTCAACCTAGAGGGCAGTTGTGAGGCACTTAATAGGTCTTGTGGGTGCTGTGGTAGCTTTATGGATGATGAGCTACAACAGGAACAATGTGAGACTGCCTGTGATTTTGCAACACACGTGTTCTGTCATCCATGTGCTCTATGCCAGGAAGGTCGTGAGATCCGTCGCAGGCTGCCACATCCTGGTTTCAATGCTCAGCCAGTTCTGGTTATGATTCCTCCTGGGGAGCAGGCCATGGGCCGTGGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

247

Amino Acids

26.65

Weight (kDa)

5.27

Isoelectric Point (pI)

56.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 78 - 216 4.8e-17 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 671
AciI CCGC 4 cut(s) 96, 171, 481, 484
AclWI GGATC 2 cut(s) 364, 643
AcsI RAATTY 1 cut(s) 488
AcvI CACGTG 1 cut(s) 607
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 3 cut(s) 15, 177, 671
AflIII ACRYGT 2 cut(s) 604, 606
AgsI TTSAA 2 cut(s) 493, 679
AjnI CCWGG 5 cut(s) 30, 364, 633, 670, 709
AluBI AGCT 3 cut(s) 546, 562, 737
AluI AGCT 3 cut(s) 546, 562, 737
Alw21I GWGCWC 1 cut(s) 628
Alw26I GTCTC 2 cut(s) 343, 575
AlwI GGATC 2 cut(s) 364, 643
AoxI GGCC 3 cut(s) 102, 720, 727
ApeKI GCWGC 3 cut(s) 181, 282, 661
ApoI RAATTY 1 cut(s) 488
AspA2I CCTAGG 1 cut(s) 464
AspS9I GGNCC 2 cut(s) 168, 727
AsuHPI GGTGA 1 cut(s) 221
AvaII GGWCC 1 cut(s) 168
AvrII CCTAGG 1 cut(s) 464
BaeGI GKGCMC 1 cut(s) 195
BanII GRGCYC 2 cut(s) 47, 229
BbrPI CACGTG 1 cut(s) 607
BbsI GAAGAC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 3 cut(s) 168, 294, 648
BccI CCATC 3 cut(s) 170, 199, 485
BceAI ACGGC 1 cut(s) 714
BcgI CGANNNNNNTGC 4 cut(s) 46, 80, 271, 305
BciT130I CCWGG 5 cut(s) 32, 366, 635, 672, 711
BcoDI GTCTC 2 cut(s) 343, 575
BfaI CTAG 2 cut(s) 465, 497
BfmI CTRYAG 1 cut(s) 277
BglI GCCNNNNNGGC 1 cut(s) 190
BisI GCNGC 4 cut(s) 182, 283, 484, 662
BlnI CCTAGG 1 cut(s) 464
BlpI GCTNAGC 1 cut(s) 684
BlsI GCNGC 4 cut(s) 183, 284, 485, 663
Bme1390I CCNGG 5 cut(s) 32, 366, 635, 672, 711
Bme18I GGWCC 1 cut(s) 168
BmgT120I GGNCC 2 cut(s) 168, 727
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 5 cut(s) 32, 366, 635, 672, 711
BmsI GCATC 2 cut(s) 73, 252
BpiI GAAGAC 1 cut(s) 293
BpmI CTGGAG 1 cut(s) 223
Bpu1102I GCTNAGC 1 cut(s) 684
BsaAI YACGTR 1 cut(s) 607
BsaJI CCNNGG 8 cut(s) 99, 186, 365, 449, 464, 710, 723, 730
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 177, 671
Bse1I ACTGG 1 cut(s) 689
Bse3DI GCAATG 2 cut(s) 31, 346
BseBI CCWGG 5 cut(s) 32, 366, 635, 672, 711
BseDI CCNNGG 8 cut(s) 99, 186, 365, 449, 464, 710, 723, 730
BseGI GGATG 7 cut(s) 88, 133, 243, 477, 559, 616, 667
BseLI CCNNNNNNNGG 3 cut(s) 15, 177, 671
BseMI GCAATG 2 cut(s) 31, 346
BseMII CTCAG 5 cut(s) 44, 78, 242, 336, 698
BseNI ACTGG 1 cut(s) 689
BseRI GAGGAG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 195
BseXI GCAGC 3 cut(s) 168, 294, 648
BshFI GGCC 3 cut(s) 104, 722, 729
BsiHKAI GWGCWC 1 cut(s) 628
BsiSI CCGG 1 cut(s) 110
BslFI GGGAC 2 cut(s) 248, 382
BslI CCNNNNNNNGG 3 cut(s) 15, 177, 671
BsmAI GTCTC 2 cut(s) 343, 575
BsmFI GGGAC 2 cut(s) 248, 382
BsnI GGCC 3 cut(s) 104, 722, 729
Bsp1286I GDGCHC 4 cut(s) 47, 195, 229, 628
Bsp143I GATC 3 cut(s) 286, 356, 648
Bsp1720I GCTNAGC 1 cut(s) 684
Bsp19I CCATGG 1 cut(s) 723
BspACI CCGC 4 cut(s) 96, 171, 481, 484
BspANI GGCC 3 cut(s) 104, 722, 729
BspCNI CTCAG 5 cut(s) 45, 79, 241, 337, 697
BspLI GGNNCC 1 cut(s) 318
BspPI GGATC 2 cut(s) 364, 643
BsrDI GCAATG 2 cut(s) 31, 346
BsrI ACTGG 1 cut(s) 689
BssECI CCNNGG 8 cut(s) 99, 186, 365, 449, 464, 710, 723, 730
BssMI GATC 3 cut(s) 286, 356, 648
BssT1I CCWWGG 4 cut(s) 99, 186, 464, 723
Bst2UI CCWGG 5 cut(s) 32, 366, 635, 672, 711
Bst4CI ACNGT 2 cut(s) 386, 450
Bst6I CTCTTC 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 607
BstC8I GCNNGC 4 cut(s) 59, 96, 659, 720
BstDEI CTNAG 5 cut(s) 53, 87, 228, 345, 684
BstDSI CCRYGG 3 cut(s) 449, 723, 730
BstF5I GGATG 7 cut(s) 88, 133, 243, 477, 559, 616, 667
BstKTI GATC 3 cut(s) 289, 359, 651
BstMAI GTCTC 2 cut(s) 343, 575
BstMBI GATC 3 cut(s) 286, 356, 648
BstMWI GCNNNNNNNGC 2 cut(s) 190, 543
BstNI CCWGG 5 cut(s) 32, 366, 635, 672, 711
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 5 cut(s) 30, 364, 633, 670, 709
BstSFI CTRYAG 1 cut(s) 277
BstSLI GKGCMC 1 cut(s) 195
BstV1I GCAGC 3 cut(s) 168, 294, 648
BstV2I GAAGAC 1 cut(s) 293
BstX2I RGATCY 2 cut(s) 356, 648
BstYI RGATCY 2 cut(s) 356, 648
BsuRI GGCC 3 cut(s) 104, 722, 729
BtgI CCRYGG 3 cut(s) 449, 723, 730
BtsCI GGATG 7 cut(s) 88, 133, 243, 477, 559, 616, 667
BtsIMutI CAGTG 2 cut(s) 236, 382
Cac8I GCNNGC 4 cut(s) 59, 96, 659, 720
Cfr13I GGNCC 2 cut(s) 168, 727
Csp6I GTAC 1 cut(s) 471
CviAII CATG 3 cut(s) 137, 621, 724
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 5 cut(s) 53, 87, 228, 345, 684
DpnI GATC 3 cut(s) 288, 358, 650
DpnII GATC 3 cut(s) 286, 356, 648
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
EciI GGCGGA 1 cut(s) 470
Eco130I CCWWGG 4 cut(s) 99, 186, 464, 723
Eco147I AGGCCT 1 cut(s) 104
Eco24I GRGCYC 2 cut(s) 47, 229
Eco47I GGWCC 1 cut(s) 168
Eco72I CACGTG 1 cut(s) 607
EcoRII CCWGG 5 cut(s) 30, 364, 633, 670, 709
EcoT14I CCWWGG 4 cut(s) 99, 186, 464, 723
EcoT38I GRGCYC 2 cut(s) 47, 229
ErhI CCWWGG 4 cut(s) 99, 186, 464, 723
FaeI CATG 3 cut(s) 140, 624, 727
FalI AAGNNNNNCTT 2 cut(s) 285, 317
FaqI GGGAC 2 cut(s) 248, 382
FatI CATG 3 cut(s) 136, 620, 723
FauI CCCGC 1 cut(s) 103
Fnu4HI GCNGC 4 cut(s) 182, 283, 484, 662
FokI GGATG 7 cut(s) 95, 140, 230, 464, 566, 603, 654
FriOI GRGCYC 2 cut(s) 47, 229
Fsp4HI GCNGC 4 cut(s) 182, 283, 484, 662
FspBI CTAG 2 cut(s) 465, 497
GluI GCNGC 4 cut(s) 182, 283, 484, 662
GsuI CTGGAG 1 cut(s) 223
HaeIII GGCC 3 cut(s) 104, 722, 729
HapII CCGG 1 cut(s) 110
Hin1II CATG 3 cut(s) 140, 624, 727
HinfI GANTC 3 cut(s) 38, 236, 703
HpaII CCGG 1 cut(s) 110
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 2 cut(s) 54, 411
Hpy188III TCNNGA 2 cut(s) 290, 644
Hpy8I GTNNAC 1 cut(s) 168
Hpy99I CGWCG 2 cut(s) 127, 657
HpyAV CCTTC 2 cut(s) 73, 632
HpyCH4III ACNGT 2 cut(s) 386, 450
HpyCH4IV ACGT 2 cut(s) 371, 606
HpyCH4V TGCA 3 cut(s) 377, 446, 599
HpyF10VI GCNNNNNNNGC 2 cut(s) 190, 543
HpyF3I CTNAG 5 cut(s) 53, 87, 228, 345, 684
HpySE526I ACGT 2 cut(s) 371, 606
Hsp92II CATG 3 cut(s) 140, 624, 727
Kzo9I GATC 3 cut(s) 286, 356, 648
LmnI GCTCC 5 cut(s) 198, 322, 367, 715, 734
Lsp1109I GCAGC 3 cut(s) 168, 294, 648
LweI GCATC 2 cut(s) 73, 252
MaeI CTAG 2 cut(s) 465, 497
MaeII ACGT 2 cut(s) 371, 606
MalI GATC 3 cut(s) 288, 358, 650
MboI GATC 3 cut(s) 286, 356, 648
MboII GAAGA 2 cut(s) 41, 293
MflI RGATCY 2 cut(s) 356, 648
MhlI GDGCHC 4 cut(s) 47, 195, 229, 628
MluCI AATT 2 cut(s) 62, 488
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 413
MnlI CCTC 8 cut(s) 28, 57, 61, 115, 140, 493, 505, 717
MseI TTAA 1 cut(s) 519
MslI CAYNNNNRTG 2 cut(s) 141, 621
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 5 cut(s) 32, 366, 635, 672, 711
MvaI CCWGG 5 cut(s) 32, 366, 635, 672, 711
MwoI GCNNNNNNNGC 2 cut(s) 190, 543
NcoI CCATGG 1 cut(s) 723
NdeII GATC 3 cut(s) 286, 356, 648
NlaIII CATG 3 cut(s) 140, 624, 727
NlaIV GGNNCC 1 cut(s) 318
NspI RCATGY 1 cut(s) 140
PceI AGGCCT 1 cut(s) 104
PcsI WCGNNNNNNNCGW 1 cut(s) 649
PfeI GAWTC 2 cut(s) 38, 703
PflMI CCANNNNNTGG 1 cut(s) 671
PfoI TCCNGGA 1 cut(s) 30
PkrI GCNGC 4 cut(s) 183, 284, 485, 663
PleI GAGTC 1 cut(s) 230
PmaCI CACGTG 1 cut(s) 607
PmlI CACGTG 1 cut(s) 607
PpsI GAGTC 1 cut(s) 230
Ppu21I YACGTR 1 cut(s) 607
Psp6I CCWGG 5 cut(s) 30, 364, 633, 670, 709
PspCI CACGTG 1 cut(s) 607
PspGI CCWGG 5 cut(s) 30, 364, 633, 670, 709
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 2 cut(s) 168, 727
PsuI RGATCY 2 cut(s) 356, 648
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 2 cut(s) 141, 621
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 4 cut(s) 182, 283, 484, 662
Sau3AI GATC 3 cut(s) 286, 356, 648
Sau96I GGNCC 2 cut(s) 168, 727
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 5 cut(s) 32, 366, 635, 672, 711
SduI GDGCHC 4 cut(s) 47, 195, 229, 628
SetI ASST 9 cut(s) 151, 374, 498, 527, 548, 564, 609, 643, 739
SfaNI GCATC 2 cut(s) 73, 252
SfcI CTRYAG 1 cut(s) 277
SinI GGWCC 1 cut(s) 168
SmiMI CAYNNNNRTG 2 cut(s) 141, 621
Sse9I AATT 2 cut(s) 62, 488
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 4 cut(s) 96, 171, 481, 484
SspMI CTAG 2 cut(s) 465, 497
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 5 cut(s) 30, 364, 633, 670, 709
StyI CCWWGG 4 cut(s) 99, 186, 464, 723
TaaI ACNGT 2 cut(s) 386, 450
TaiI ACGT 2 cut(s) 374, 609
TaqI TCGA 3 cut(s) 41, 66, 291
TasI AATT 2 cut(s) 62, 488
TauI GCSGC 1 cut(s) 486
TfiI GAWTC 2 cut(s) 38, 703
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TscAI CASTG 2 cut(s) 236, 389
TseI GCWGC 3 cut(s) 181, 282, 661
TspDTI ATGAA 1 cut(s) 447
TspGWI ACGGA 2 cut(s) 140, 641
TspRI CASTG 2 cut(s) 236, 389
Van91I CCANNNNNTGG 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 168
XapI RAATTY 1 cut(s) 488
XceI RCATGY 1 cut(s) 140
XmaJI CCTAGG 1 cut(s) 464
XspI CTAG 2 cut(s) 465, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.