MD12G1121900.v1.1

Cell number regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009470
Physical Location & Seq
Reverse (-)
19569259 .. 19571722
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1121900.v1.1.491

Sequence Viewer

Length: 756 bp
ATGGCGAGCAACAACAGGGAGAATAATGTCCAGGAGGAATCGAACCCGCTTCTGGGCAAGCAATTCGAGGAGACCCAGAATGATAATGAGAAGCCCGCCAAGGCCTCGCCGGAGACTCAGTCGCCGGAGAAATCGCCCACGGAGATGGGGCATGTGAATGGCGGTGGTAATGGGTGCGCGTGGACCGCCGATGGGCTGCCTTTGGGGCACGGGAGCGTGATGGGTGAGCCCATGGGCCGGGCCCAGTGGGACTCCAGCCTCCTTGCCTGTCTTGGCCGCAATGATGAATTCTGTAGCAGCGATCTTGAAGTTTGCCTTCTTGGAAGTGTGGCTCCTTGTGTGTTGTATGGAAGTAATGCTGAGAGACTTGGATCTACTCCTGGGACGTTTGCAAATCACTGTTTGCCTTACTCTGGTCTATATCTGATTGGAAACACCTTTTTTGGTTGGAACTTCCTTGCACCGTGGTTTTCATATCCTAGCCGTACTGCCATCCGCCGCAAGTTCAACCTAGAGGGAAGTTGTGAGGCACTTAATAGGTCATGTGGCTGCTGCGGAAGCTTTGTGGATGATGAGCTACAACAGGAACAGTGTGAGACTGCCTGTGATTTTGCGACTCATGTGTTCTGCCATCCATGTGCTCTATGCCAGGAAGGTCGCGAGATCCGTCGCAGGCTGCCTCATCCTGGTTTCAATGCGCAGCCGGTTCTGGTTATGATTCCTCCTGAGGAGCAGGGCATGGGCCGTGGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

252

Amino Acids

27.29

Weight (kDa)

4.93

Isoelectric Point (pI)

56.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 83 - 221 1.3e-17 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 699
AccII CGCG 2 cut(s) 179, 660
AciI CCGC 8 cut(s) 47, 96, 162, 186, 277, 496, 499, 555
AclWI GGATC 2 cut(s) 379, 658
AcoI YGGCCR 1 cut(s) 274
AcsI RAATTY 1 cut(s) 287
AfaI GTAC 1 cut(s) 487
AfiI CCNNNNNNNGG 6 cut(s) 52, 53, 192, 237, 413, 686
AgsI TTSAA 3 cut(s) 308, 508, 694
AjnI CCWGG 4 cut(s) 30, 379, 648, 685
AluBI AGCT 2 cut(s) 561, 577
AluI AGCT 2 cut(s) 561, 577
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 4 cut(s) 65, 107, 358, 590
AlwI GGATC 2 cut(s) 379, 658
AoxI GGCC 5 cut(s) 102, 235, 240, 274, 742
ApaI GGGCCC 1 cut(s) 244
ApeKI GCWGC 6 cut(s) 196, 297, 549, 552, 676, 700
ApoI RAATTY 1 cut(s) 287
AspLEI GCGC 2 cut(s) 179, 700
AspS9I GGNCC 5 cut(s) 183, 235, 240, 241, 742
AsuC2I CCSGG 1 cut(s) 239
AsuHPI GGTGA 1 cut(s) 236
AvaII GGWCC 1 cut(s) 183
AxyI CCTNAGG 1 cut(s) 726
BaeGI GKGCMC 2 cut(s) 210, 244
BanII GRGCYC 2 cut(s) 231, 244
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 6 cut(s) 183, 309, 536, 539, 663, 712
BccI CCATC 5 cut(s) 139, 185, 214, 500, 639
BceAI ACGGC 2 cut(s) 468, 729
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BciT130I CCWGG 4 cut(s) 32, 381, 650, 687
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 4 cut(s) 65, 107, 358, 590
BfaI CTAG 2 cut(s) 480, 512
BfmI CTRYAG 1 cut(s) 292
BglI GCCNNNNNGGC 1 cut(s) 205
BisI GCNGC 8 cut(s) 197, 277, 298, 499, 550, 553, 677, 701
BlsI GCNGC 8 cut(s) 198, 278, 299, 500, 551, 554, 678, 702
Bme1390I CCNGG 5 cut(s) 32, 239, 381, 650, 687
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 5 cut(s) 183, 235, 240, 241, 742
BmiI GGNNCC 2 cut(s) 242, 333
BmrFI CCNGG 5 cut(s) 32, 239, 381, 650, 687
BmrI ACTGGG 1 cut(s) 238
BmuI ACTGGG 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 238
BpuMI CCSGG 1 cut(s) 239
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 6 cut(s) 99, 138, 231, 380, 464, 745
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 53, 192, 237, 413, 686
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 1 cut(s) 244
Bse21I CCTNAGG 1 cut(s) 726
Bse3DI GCAATG 1 cut(s) 286
BseBI CCWGG 4 cut(s) 32, 381, 650, 687
BseDI CCNNGG 6 cut(s) 99, 138, 231, 380, 464, 745
BseGI GGATG 4 cut(s) 492, 574, 631, 682
BseLI CCNNNNNNNGG 6 cut(s) 52, 53, 192, 237, 413, 686
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 3 cut(s) 131, 351, 717
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 2 cut(s) 83, 743
BseSI GKGCMC 2 cut(s) 210, 244
BseXI GCAGC 6 cut(s) 183, 309, 536, 539, 663, 712
Bsh1236I CGCG 2 cut(s) 179, 660
BshFI GGCC 5 cut(s) 104, 237, 242, 276, 744
BsiHKAI GWGCWC 1 cut(s) 643
BsiSI CCGG 4 cut(s) 110, 125, 238, 704
BslFI GGGAC 2 cut(s) 263, 397
BslI CCNNNNNNNGG 6 cut(s) 52, 53, 192, 237, 413, 686
BsmAI GTCTC 4 cut(s) 65, 107, 358, 590
BsmFI GGGAC 2 cut(s) 263, 397
BsnI GGCC 5 cut(s) 104, 237, 242, 276, 744
Bso31I GGTCTC 1 cut(s) 65
Bsp120I GGGCCC 1 cut(s) 240
Bsp1286I GDGCHC 4 cut(s) 210, 231, 244, 643
Bsp143I GATC 3 cut(s) 301, 371, 663
Bsp19I CCATGG 1 cut(s) 231
Bsp68I TCGCGA 1 cut(s) 660
BspACI CCGC 8 cut(s) 47, 96, 162, 186, 277, 496, 499, 555
BspANI GGCC 5 cut(s) 104, 237, 242, 276, 744
BspCNI CTCAG 3 cut(s) 130, 352, 718
BspFNI CGCG 2 cut(s) 179, 660
BspLI GGNNCC 2 cut(s) 242, 333
BspPI GGATC 2 cut(s) 379, 658
BspTNI GGTCTC 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 286
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 1 cut(s) 244
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 6 cut(s) 99, 138, 231, 380, 464, 745
BssMI GATC 3 cut(s) 301, 371, 663
BssT1I CCWWGG 2 cut(s) 99, 231
Bst2UI CCWGG 4 cut(s) 32, 381, 650, 687
Bst4CI ACNGT 3 cut(s) 401, 465, 591
BstC8I GCNNGC 4 cut(s) 7, 59, 96, 674
BstDEI CTNAG 3 cut(s) 117, 360, 726
BstDSI CCRYGG 4 cut(s) 138, 231, 464, 745
BstF5I GGATG 4 cut(s) 492, 574, 631, 682
BstFNI CGCG 2 cut(s) 179, 660
BstHHI GCGC 2 cut(s) 179, 700
BstKTI GATC 3 cut(s) 304, 374, 666
BstMAI GTCTC 4 cut(s) 65, 107, 358, 590
BstMBI GATC 3 cut(s) 301, 371, 663
BstMWI GCNNNNNNNGC 3 cut(s) 185, 205, 558
BstNI CCWGG 4 cut(s) 32, 381, 650, 687
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 5 cut(s) 30, 237, 379, 648, 685
BstSFI CTRYAG 1 cut(s) 292
BstSLI GKGCMC 2 cut(s) 210, 244
BstUI CGCG 2 cut(s) 179, 660
BstV1I GCAGC 6 cut(s) 183, 309, 536, 539, 663, 712
BstX2I RGATCY 2 cut(s) 371, 663
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 2 cut(s) 371, 663
Bsu36I CCTNAGG 1 cut(s) 726
BsuRI GGCC 5 cut(s) 104, 237, 242, 276, 744
BtgI CCRYGG 4 cut(s) 138, 231, 464, 745
BtsCI GGATG 4 cut(s) 492, 574, 631, 682
BtsIMutI CAGTG 3 cut(s) 251, 397, 596
BtuMI TCGCGA 1 cut(s) 660
Cac8I GCNNGC 4 cut(s) 7, 59, 96, 674
CfoI GCGC 2 cut(s) 179, 700
Cfr10I RCCGGY 1 cut(s) 703
Cfr13I GGNCC 5 cut(s) 183, 235, 240, 241, 742
Csp6I GTAC 1 cut(s) 486
CviAII CATG 6 cut(s) 152, 232, 543, 620, 636, 739
CviQI GTAC 1 cut(s) 486
DdeI CTNAG 3 cut(s) 117, 360, 726
DpnI GATC 3 cut(s) 303, 373, 665
DpnII GATC 3 cut(s) 301, 371, 663
EaeI YGGCCR 1 cut(s) 274
EciI GGCGGA 1 cut(s) 485
Eco130I CCWWGG 2 cut(s) 99, 231
Eco147I AGGCCT 1 cut(s) 104
Eco24I GRGCYC 2 cut(s) 231, 244
Eco31I GGTCTC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 183
Eco81I CCTNAGG 1 cut(s) 726
EcoRI GAATTC 1 cut(s) 287
EcoRII CCWGG 4 cut(s) 30, 379, 648, 685
EcoT14I CCWWGG 2 cut(s) 99, 231
EcoT38I GRGCYC 2 cut(s) 231, 244
ErhI CCWWGG 2 cut(s) 99, 231
FaeI CATG 6 cut(s) 155, 235, 546, 623, 639, 742
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FaqI GGGAC 2 cut(s) 263, 397
FatI CATG 6 cut(s) 151, 231, 542, 619, 635, 738
FauI CCCGC 2 cut(s) 54, 103
Fnu4HI GCNGC 8 cut(s) 197, 277, 298, 499, 550, 553, 677, 701
FokI GGATG 4 cut(s) 479, 581, 618, 669
FriOI GRGCYC 2 cut(s) 231, 244
Fsp4HI GCNGC 8 cut(s) 197, 277, 298, 499, 550, 553, 677, 701
FspBI CTAG 2 cut(s) 480, 512
FspI TGCGCA 1 cut(s) 699
GlaI GCGC 2 cut(s) 178, 699
GluI GCNGC 8 cut(s) 197, 277, 298, 499, 550, 553, 677, 701
GsuI CTGGAG 1 cut(s) 238
HaeIII GGCC 5 cut(s) 104, 237, 242, 276, 744
HapII CCGG 4 cut(s) 110, 125, 238, 704
HhaI GCGC 2 cut(s) 179, 700
Hin1II CATG 6 cut(s) 155, 235, 546, 623, 639, 742
Hin6I GCGC 2 cut(s) 177, 698
HinP1I GCGC 2 cut(s) 177, 698
HindIII AAGCTT 1 cut(s) 559
HinfI GANTC 5 cut(s) 38, 115, 251, 616, 718
HpaII CCGG 4 cut(s) 110, 125, 238, 704
HphI GGTGA 1 cut(s) 236
Hpy166II GTNNAC 1 cut(s) 183
Hpy188I TCNGA 1 cut(s) 426
Hpy188III TCNNGA 3 cut(s) 305, 659, 725
Hpy8I GTNNAC 1 cut(s) 183
Hpy99I CGWCG 1 cut(s) 672
HpyAV CCTTC 2 cut(s) 326, 647
HpyCH4III ACNGT 3 cut(s) 401, 465, 591
HpyCH4IV ACGT 1 cut(s) 386
HpyCH4V TGCA 2 cut(s) 392, 461
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 205, 558
HpyF3I CTNAG 3 cut(s) 117, 360, 726
HpySE526I ACGT 1 cut(s) 386
Hsp92II CATG 6 cut(s) 155, 235, 546, 623, 639, 742
HspAI GCGC 2 cut(s) 177, 698
Kzo9I GATC 3 cut(s) 301, 371, 663
LmnI GCTCC 3 cut(s) 213, 337, 730
Lsp1109I GCAGC 6 cut(s) 183, 309, 536, 539, 663, 712
MaeI CTAG 2 cut(s) 480, 512
MaeII ACGT 1 cut(s) 386
MalI GATC 3 cut(s) 303, 373, 665
MboI GATC 3 cut(s) 301, 371, 663
MflI RGATCY 2 cut(s) 371, 663
MhlI GDGCHC 4 cut(s) 210, 231, 244, 643
MluCI AATT 2 cut(s) 62, 287
MlyI GAGTC 3 cut(s) 109, 245, 610
MmeI TCCRAC 1 cut(s) 428
MnlI CCTC 9 cut(s) 28, 61, 115, 269, 508, 520, 690, 721, 732
MseI TTAA 1 cut(s) 534
MslI CAYNNNNRTG 3 cut(s) 143, 156, 636
MspI CCGG 4 cut(s) 110, 125, 238, 704
MspR9I CCNGG 5 cut(s) 32, 239, 381, 650, 687
MvaI CCWGG 4 cut(s) 32, 381, 650, 687
MvnI CGCG 2 cut(s) 179, 660
MwoI GCNNNNNNNGC 3 cut(s) 185, 205, 558
NciI CCSGG 1 cut(s) 239
NcoI CCATGG 1 cut(s) 231
NdeII GATC 3 cut(s) 301, 371, 663
NlaIII CATG 6 cut(s) 155, 235, 546, 623, 639, 742
NlaIV GGNNCC 2 cut(s) 242, 333
NruI TCGCGA 1 cut(s) 660
NsbI TGCGCA 1 cut(s) 699
NspI RCATGY 1 cut(s) 155
PceI AGGCCT 1 cut(s) 104
PcsI WCGNNNNNNNCGW 1 cut(s) 664
PfeI GAWTC 2 cut(s) 38, 718
PflFI GACNNNGTC 1 cut(s) 118
PfoI TCCNGGA 1 cut(s) 30
PkrI GCNGC 8 cut(s) 198, 278, 299, 500, 551, 554, 678, 702
PleI GAGTC 3 cut(s) 109, 245, 610
PpsI GAGTC 3 cut(s) 109, 245, 610
Psp6I CCWGG 4 cut(s) 30, 379, 648, 685
PspGI CCWGG 4 cut(s) 30, 379, 648, 685
PspN4I GGNNCC 2 cut(s) 242, 333
PspOMI GGGCCC 1 cut(s) 240
PspPI GGNCC 5 cut(s) 183, 235, 240, 241, 742
PsuI RGATCY 2 cut(s) 371, 663
PsyI GACNNNGTC 1 cut(s) 118
RruI TCGCGA 1 cut(s) 660
RsaI GTAC 1 cut(s) 487
RsaNI GTAC 1 cut(s) 486
RseI CAYNNNNRTG 3 cut(s) 143, 156, 636
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 8 cut(s) 197, 277, 298, 499, 550, 553, 677, 701
Sau3AI GATC 3 cut(s) 301, 371, 663
Sau96I GGNCC 5 cut(s) 183, 235, 240, 241, 742
SchI GAGTC 3 cut(s) 109, 245, 610
ScrFI CCNGG 5 cut(s) 32, 239, 381, 650, 687
SduI GDGCHC 4 cut(s) 210, 231, 244, 643
SetI ASST 7 cut(s) 389, 440, 513, 542, 563, 579, 658
SfcI CTRYAG 1 cut(s) 292
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 3 cut(s) 143, 156, 636
Sse9I AATT 2 cut(s) 62, 287
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 8 cut(s) 47, 96, 162, 186, 277, 496, 499, 555
SspMI CTAG 2 cut(s) 480, 512
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 5 cut(s) 30, 237, 379, 648, 685
StyI CCWWGG 2 cut(s) 99, 231
TaaI ACNGT 3 cut(s) 401, 465, 591
TaiI ACGT 1 cut(s) 389
TaqI TCGA 2 cut(s) 41, 66
TasI AATT 2 cut(s) 62, 287
TauI GCSGC 2 cut(s) 279, 501
TfiI GAWTC 2 cut(s) 38, 718
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TscAI CASTG 3 cut(s) 251, 404, 596
TseI GCWGC 6 cut(s) 196, 297, 549, 552, 676, 700
TspDTI ATGAA 2 cut(s) 300, 462
TspGWI ACGGA 2 cut(s) 155, 656
TspRI CASTG 3 cut(s) 251, 404, 596
Tth111I GACNNNGTC 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 183
XapI RAATTY 1 cut(s) 287
XceI RCATGY 1 cut(s) 155
XspI CTAG 2 cut(s) 480, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.