Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001825

Physical Location & Seq

Genomic Coordinates Reverse (-)

19104208 .. 19108146

Gene Structure 3939 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1103800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGTCGAAGGTGAGAGGATCGAGCTCGTGGCGGGACGAGCTGGCGAGTCTGGTGAGTGACTCGGGGATAAGATTCTCGGCTTCCGATCCTATTGAGGTCTCCACGGCGTCGTTTGAGGCCGGTTTCGCCTCCGGCGGAGAATTGGAGCAATCGGAGAGCTTCAAGGATCAGATTAAGGGATTCGCGATCGCTTGGGGCGAAATACTTGTGGATTTGGCAAGAGGCTGTAAGGACATTGTGGAGCAGAACGTTCTGACTGAGGACTCGTACATTGTACGAAAGCTTCGGAAGCCCTGCGCCAAGGCGTGGGCGAAATTGAGATACTTGAATGAGTTTTTGCCGGAGGATCGCGATCCGGCTCACGCGTGGCCGGTGATATTGTTCGTGTTCATTCTTGCGCTTACAGCTATGAATCTCAATACCAAGAATGACAGCTTGGTCCGATCAGTGAAGAAAGTACAAATACATCCTCCTAGTGCTAGTCATGTACTACTTCCAGATGGGAGACGATTGGCCTATCAAGAACAAGGAGTCCCAGCAAAGAGCACTCGATTTTTACTGATTGCTCCACATTCTTTTCTTTCATCCCGACTTGCAGGTATACCAGGAATAAGGATATCATTGCTTGAAGAGTTTGGTATCCGCTTGGTCACATATGATCTTCCTGGTTTTGGGGAGAGTGATCCTCATCCCAGCAGGAATCTTAACTCGTCGGCATTGGATATGCTATACTTAGCAAATGCTATTGGTGTTGATGACAAGTTCTGGGTGCTGGGCCACTCAAGTGGAACGATGCATGCTTGGGCTTCTCTCAGATACATTCCTGACAGAGTTGCAGGTGCAGCCATGGTGGCCCCAATAATTAATCCCTATGAACCGAGCATGACTAAAGAGGAGATGAAAAAGACTTGGGAACCATGGGTTCTGCGAAGGAGAGTGATGTTTTTTTTAGCTCGTAGATTTCCAACATTTCTCTCCTCTTTTTATTGCCGAAGCTTCCTATCAGGAAAACACGACAGAATTGATAAGTGGTTGTCTCTCTCACTGGGGAAGAAGGATGAAGTTCTAATTGAAGATCCAAAAATTGAGGAATATTTGCAAAGGGATATAGAGGAGTCGATCCGCCAAGGGAGTATGAAACCCTTTATAGAGGAAGCTGTCCTACAAGTATCACGGTGGGGTTTTAGTCTGGCAGATCTTCGGGCACAGAAACAGTGTCAACGAAGAGGATTTCTTGCTTGGCTCTGGAACAGTGAGGCAGAATGCATGCTGACTGGATTTCTAGGCCAGATACACATATGGCAGGGGATGGATGATATGGTAATCCCACAATCAGTGACCGACTATATAGCACGGGTTTTGCCTGGATCTTACGTGCATAAGCTCCCAAATGAAGGCCACTTCTCGTACTTATACTTCTGTGATGAATGCCACAGACAGATGTTCACTACCCTTTTTGGAAGCCCTCAAGGTCCAGTAGTCGAAAAAGTGGATATAGATACAACGCCATCGAAAGGCAACTCAGAAGAGATATCAATGAATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6

Protein Analysis

516

Amino Acids

58.23

Weight (kDa)

6.34

Isoelectric Point (pI)

52.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_1	PF00561	208 - 471	1.9e-18	alpha/beta hydrolase fold
Hydrolase_4	PF12146	209 - 301	1.4e-06	Serine aminopeptidase, S33
Abhydrolase_6	PF12697	212 - 471	3.3e-11	Alpha/beta hydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001825)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09690 AT3G09690 AT5G02970
fragaria_vesca	FvH4_3g33440 FvH4_3g33440 FvH4_3g33440 FvH4_3g33440 FvH4_6g15410 FvH4_6g15410 FvH4_6g15410 FvH4_6g15410 FvH4_6g15410
malus_domestica	MD03G1111700.v1.1 MD04G1103800.v1.1 MD11G1126200.v1.1
prunus_persica	Prupe.6G094600_v2.0.a1 Prupe.6G240800_v2.0.a1 Prupe.6G240800_v2.0.a1 Prupe.6G240800_v2.0.a1 Prupe.6G240800_v2.0.a1
pyrus_communis	pycom04g09900 pycom11g10650 pycom12g12240
rosa_chinensis	RchiOBHm_Chr3g0468891 RchiOBHm_Chr5g0060781
rosa_laevigata	RLG00000024403 RLG00000035400
rosa_multiflora	Rmu_co8389401.1_g000001 Rmu_sc0000727.1_g000032 Rmu_sc0001639.1_g000013 Rmu_sc0003298.1_g000004 Rmu_sc0007143.1_g000009
rosa_roxburghii	Rroxscaffold_1G00019940 Rroxscaffold_6G00412480
rosa_rugosa	Rorug03G0097200 Rorug03G0097300 Rorug03G0097400 Rorug05G0334000
rosa_samantha	Rh3AG147000 Rh3BG169500 Rh3DG205600 Rh5AG396700 Rh5BG408700 Rh5CG433400 Rh5DG424000
rosa_wichuraiana	Rw3G013830 Rw5G037400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	827
AasI	GACNNNNNNGTC	1 cut(s)	437
Acc36I	ACCTGC	2 cut(s)	587, 827
AccB7I	CCANNNNNTGG	1 cut(s)	306
AccI	GTMKAC	1 cut(s)	601
AccII	CGCG	3 cut(s)	185, 351, 365
AciI	CCGC	4 cut(s)	31, 135, 643, 1123
AclI	AACGTT	1 cut(s)	249
AclWI	GGATC	9 cut(s)	25, 80, 174, 347, 354, 677, 1070, 1114, 1375
AcoI	YGGCCR	1 cut(s)	368
AcyI	GRCGYC	1 cut(s)	107
AfaI	GTAC	5 cut(s)	269, 276, 459, 489, 1409
AfiI	CCNNNNNNNGG	4 cut(s)	306, 671, 1394, 1514
AflIII	ACRYGT	1 cut(s)	363
AgsI	TTSAA	4 cut(s)	163, 328, 629, 1073
AjnI	CCWGG	3 cut(s)	604, 664, 1363
AjuI	GAANNNNNNNTTGG	2 cut(s)	419, 451
AleI	CACNNNNGTG	1 cut(s)	783
AloI	GAACNNNNNNTCC	2 cut(s)	516, 548
Alw21I	GWGCWC	2 cut(s)	26, 548
Alw26I	GTCTC	3 cut(s)	103, 499, 1041
AlwI	GGATC	9 cut(s)	25, 80, 174, 347, 354, 677, 1070, 1114, 1375
Ama87I	CYCGRG	1 cut(s)	61
AoxI	GGCC	7 cut(s)	117, 368, 513, 775, 852, 1285, 1396
ApeKI	GCWGC	1 cut(s)	842
AseI	ATTAAT	1 cut(s)	864
AsiSI	GCGATCGC	1 cut(s)	189
AspLEI	GCGC	2 cut(s)	299, 400
AspS9I	GGNCC	4 cut(s)	439, 775, 853, 1472
AsuHPI	GGTGA	3 cut(s)	22, 64, 385
AvaI	CYCGRG	1 cut(s)	61
AvaII	GGWCC	2 cut(s)	439, 1472
BaeGI	GKGCMC	1 cut(s)	1207
BanII	GRGCYC	1 cut(s)	26
BarI	GAAGNNNNNNTAC	6 cut(s)	267, 299, 1146, 1178, 1400, 1432
BauI	CACGAG	1 cut(s)	25
Bbv12I	GWGCWC	2 cut(s)	26, 548
BbvI	GCAGC	1 cut(s)	854
BccI	CCATC	3 cut(s)	494, 1303, 1516
BceAI	ACGGC	1 cut(s)	120
BciT130I	CCWGG	3 cut(s)	606, 666, 1365
BciVI	GTATCC	1 cut(s)	650
BcoDI	GTCTC	3 cut(s)	103, 499, 1041
BfaI	CTAG	3 cut(s)	474, 480, 1283
BfuAI	ACCTGC	2 cut(s)	587, 827
BfuI	GTATCC	1 cut(s)	650
BglI	GCCNNNNNGGC	1 cut(s)	851
BglII	AGATCT	1 cut(s)	1195
BisI	GCNGC	1 cut(s)	843
BlsI	GCNGC	1 cut(s)	844
Bme1390I	CCNGG	3 cut(s)	606, 666, 1365
Bme18I	GGWCC	2 cut(s)	439, 1472
BmeT110I	CYCGRG	1 cut(s)	61
BmgT120I	GGNCC	4 cut(s)	439, 775, 853, 1472
BmiI	GGNNCC	2 cut(s)	855, 915
BmrFI	CCNGG	3 cut(s)	606, 666, 1365
BmrI	ACTGGG	1 cut(s)	1055
BmsI	GCATC	1 cut(s)	783
BmuI	ACTGGG	1 cut(s)	1055
BplI	GAGNNNNNCTC	2 cut(s)	670, 702
BpuEI	CTTGAG	2 cut(s)	766, 1452
BsaAI	YACGTR	1 cut(s)	1375
BsaBI	GATNNNNATC	2 cut(s)	351, 687
BsaHI	GRCGYC	1 cut(s)	107
BsaI	GGTCTC	1 cut(s)	103
BsaJI	CCNNGG	5 cut(s)	102, 300, 846, 917, 1126
Bsc4I	CCNNNNNNNGG	4 cut(s)	306, 671, 1394, 1514
Bse118I	RCCGGY	2 cut(s)	119, 370
Bse1I	ACTGG	3 cut(s)	1050, 1279, 1475
Bse3DI	GCAATG	1 cut(s)	620
Bse8I	GATNNNNATC	2 cut(s)	351, 687
BseBI	CCWGG	3 cut(s)	606, 666, 1365
BseDI	CCNNGG	5 cut(s)	102, 300, 846, 917, 1126
BseGI	GGATG	6 cut(s)	466, 584, 688, 1063, 1314, 1318
BseJI	GATNNNNATC	2 cut(s)	351, 687
BseLI	CCNNNNNNNGG	4 cut(s)	306, 671, 1394, 1514
BseMI	GCAATG	1 cut(s)	620
BseMII	CTCAG	3 cut(s)	249, 826, 1536
BseNI	ACTGG	3 cut(s)	1050, 1279, 1475
BseRI	GAGGAG	3 cut(s)	908, 967, 1127
BseSI	GKGCMC	1 cut(s)	1207
BseXI	GCAGC	1 cut(s)	854
BseYI	CCCAGC	3 cut(s)	535, 692, 772
BsgI	GTGCAG	1 cut(s)	861
Bsh1236I	CGCG	3 cut(s)	185, 351, 365
Bsh1285I	CGRYCG	1 cut(s)	189
BshFI	GGCC	7 cut(s)	119, 370, 515, 777, 854, 1287, 1398
BsiEI	CGRYCG	1 cut(s)	189
BsiHKAI	GWGCWC	2 cut(s)	26, 548
BsiHKCI	CYCGRG	1 cut(s)	61
BsiSI	CCGG	5 cut(s)	120, 132, 341, 356, 371
BslFI	GGGAC	2 cut(s)	47, 518
BslI	CCNNNNNNNGG	4 cut(s)	306, 671, 1394, 1514
BsmAI	GTCTC	3 cut(s)	103, 499, 1041
BsmBI	CGTCTC	1 cut(s)	499
BsmFI	GGGAC	2 cut(s)	47, 518
BsmI	GAATGC	3 cut(s)	1268, 1433, 1546
BsnI	GGCC	7 cut(s)	119, 370, 515, 777, 854, 1287, 1398
Bso31I	GGTCTC	1 cut(s)	103
BsoBI	CYCGRG	1 cut(s)	61
Bsp1286I	GDGCHC	3 cut(s)	26, 548, 1207
Bsp19I	CCATGG	2 cut(s)	846, 917
Bsp68I	TCGCGA	2 cut(s)	185, 351
BspACI	CCGC	4 cut(s)	31, 135, 643, 1123
BspANI	GGCC	7 cut(s)	119, 370, 515, 777, 854, 1287, 1398
BspCNI	CTCAG	3 cut(s)	250, 825, 1535
BspFNI	CGCG	3 cut(s)	185, 351, 365
BspLI	GGNNCC	2 cut(s)	855, 915
BspMI	ACCTGC	2 cut(s)	587, 827
BspPI	GGATC	9 cut(s)	25, 80, 174, 347, 354, 677, 1070, 1114, 1375
BspTNI	GGTCTC	1 cut(s)	103
BsrDI	GCAATG	1 cut(s)	620
BsrFI	RCCGGY	2 cut(s)	119, 370
BsrI	ACTGG	3 cut(s)	1050, 1279, 1475
BssAI	RCCGGY	2 cut(s)	119, 370
BssECI	CCNNGG	5 cut(s)	102, 300, 846, 917, 1126
BssNAI	GTATAC	1 cut(s)	602
BssNI	GRCGYC	1 cut(s)	107
BssSI	CACGAG	1 cut(s)	25
BssT1I	CCWWGG	4 cut(s)	300, 846, 917, 1126
Bst1107I	GTATAC	1 cut(s)	602
Bst2BI	CACGAG	1 cut(s)	25
Bst2UI	CCWGG	3 cut(s)	606, 666, 1365
Bst4CI	ACNGT	3 cut(s)	1176, 1215, 1253
Bst6I	CTCTTC	3 cut(s)	624, 1219, 1521
BstACI	GRCGYC	1 cut(s)	107
BstBAI	YACGTR	1 cut(s)	1375
BstC8I	GCNNGC	3 cut(s)	42, 798, 1268
BstDEI	CTNAG	4 cut(s)	258, 733, 812, 1522
BstDSI	CCRYGG	3 cut(s)	102, 846, 917
BstF5I	GGATG	6 cut(s)	466, 584, 688, 1063, 1314, 1318
BstFNI	CGCG	3 cut(s)	185, 351, 365
BstHHI	GCGC	2 cut(s)	299, 400
BstMAI	GTCTC	3 cut(s)	103, 499, 1041
BstMCI	CGRYCG	1 cut(s)	189
BstMWI	GCNNNNNNNGC	6 cut(s)	37, 125, 289, 404, 842, 851
BstNI	CCWGG	3 cut(s)	606, 666, 1365
BstNSI	RCATGY	2 cut(s)	800, 1270
BstSCI	CCNGG	3 cut(s)	604, 664, 1363
BstSLI	GKGCMC	1 cut(s)	1207
BstUI	CGCG	3 cut(s)	185, 351, 365
BstV1I	GCAGC	1 cut(s)	854
BstX2I	RGATCY	3 cut(s)	1075, 1195, 1367
BstXI	CCANNNNNNTGG	1 cut(s)	785
BstYI	RGATCY	3 cut(s)	1075, 1195, 1367
BstZ17I	GTATAC	1 cut(s)	602
BsuI	GTATCC	1 cut(s)	650
BsuRI	GGCC	7 cut(s)	119, 370, 515, 777, 854, 1287, 1398
BtgI	CCRYGG	3 cut(s)	102, 846, 917
BtsCI	GGATG	6 cut(s)	466, 584, 688, 1063, 1314, 1318
BtsIMutI	CAGTG	5 cut(s)	453, 1043, 1220, 1258, 1341
BtuMI	TCGCGA	2 cut(s)	185, 351
BveI	ACCTGC	2 cut(s)	587, 827
Cac8I	GCNNGC	3 cut(s)	42, 798, 1268
CfoI	GCGC	2 cut(s)	299, 400
Cfr10I	RCCGGY	2 cut(s)	119, 370
Cfr13I	GGNCC	4 cut(s)	439, 775, 853, 1472
CseI	GACGC	1 cut(s)	96
Csp6I	GTAC	5 cut(s)	268, 275, 458, 488, 1408
CviAII	CATG	6 cut(s)	485, 797, 847, 883, 918, 1267
CviQI	GTAC	5 cut(s)	268, 275, 458, 488, 1408
DdeI	CTNAG	4 cut(s)	258, 733, 812, 1522
DrdI	GACNNNNNNGTC	1 cut(s)	437
DseDI	GACNNNNNNGTC	1 cut(s)	437
EaeI	YGGCCR	1 cut(s)	368
Eam1104I	CTCTTC	3 cut(s)	624, 1219, 1521
EarI	CTCTTC	3 cut(s)	624, 1219, 1521
EciI	GGCGGA	2 cut(s)	150, 1112
Ecl136II	GAGCTC	1 cut(s)	24
Eco130I	CCWWGG	4 cut(s)	300, 846, 917, 1126
Eco24I	GRGCYC	1 cut(s)	26
Eco31I	GGTCTC	1 cut(s)	103
Eco32I	GATATC	2 cut(s)	618, 1533
Eco47I	GGWCC	2 cut(s)	439, 1472
Eco53kI	GAGCTC	1 cut(s)	24
Eco88I	CYCGRG	1 cut(s)	61
EcoICRI	GAGCTC	1 cut(s)	24
EcoRII	CCWGG	3 cut(s)	604, 664, 1363
EcoRV	GATATC	2 cut(s)	618, 1533
EcoT14I	CCWWGG	4 cut(s)	300, 846, 917, 1126
EcoT22I	ATGCAT	2 cut(s)	798, 1268
EcoT38I	GRGCYC	1 cut(s)	26
ErhI	CCWWGG	4 cut(s)	300, 846, 917, 1126
Esp3I	CGTCTC	1 cut(s)	499
FaeI	CATG	6 cut(s)	488, 800, 850, 886, 921, 1270
FaqI	GGGAC	2 cut(s)	47, 518
FatI	CATG	6 cut(s)	484, 796, 846, 882, 917, 1266
FauI	CCCGC	1 cut(s)	24
FauNDI	CATATG	2 cut(s)	655, 1298
FblI	GTMKAC	1 cut(s)	601
Fnu4HI	GCNGC	1 cut(s)	843
FokI	GGATG	6 cut(s)	453, 571, 675, 1070, 1321, 1325
FriOI	GRGCYC	1 cut(s)	26
Fsp4HI	GCNGC	1 cut(s)	843
FspBI	CTAG	3 cut(s)	474, 480, 1283
GlaI	GCGC	2 cut(s)	298, 399
GluI	GCNGC	1 cut(s)	843
GsaI	CCCAGC	3 cut(s)	539, 696, 776
HaeIII	GGCC	7 cut(s)	119, 370, 515, 777, 854, 1287, 1398
HapII	CCGG	5 cut(s)	120, 132, 341, 356, 371
HgaI	GACGC	1 cut(s)	96
HhaI	GCGC	2 cut(s)	299, 400
Hin1I	GRCGYC	1 cut(s)	107
Hin1II	CATG	6 cut(s)	488, 800, 850, 886, 921, 1270
Hin6I	GCGC	2 cut(s)	297, 398
HinP1I	GCGC	2 cut(s)	297, 398
HincII	GTYRAC	1 cut(s)	1220
HindII	GTYRAC	1 cut(s)	1220
HindIII	AAGCTT	2 cut(s)	281, 994
HinfI	GANTC	9 cut(s)	46, 59, 72, 180, 263, 412, 531, 700, 1115
HpaII	CCGG	5 cut(s)	120, 132, 341, 356, 371
HphI	GGTGA	3 cut(s)	22, 64, 385
Hpy166II	GTNNAC	3 cut(s)	602, 1220, 1446
Hpy188I	TCNGA	8 cut(s)	85, 154, 171, 255, 288, 443, 815, 1525
Hpy188III	TCNNGA	8 cut(s)	184, 350, 497, 521, 588, 824, 1005, 1246
Hpy8I	GTNNAC	3 cut(s)	602, 1220, 1446
Hpy99I	CGWCG	2 cut(s)	112, 715
HpyAV	CCTTC	3 cut(s)	924, 1048, 1388
HpyCH4III	ACNGT	3 cut(s)	1176, 1215, 1253
HpyCH4IV	ACGT	2 cut(s)	249, 1374
HpyCH4V	TGCA	7 cut(s)	596, 796, 836, 842, 1099, 1266, 1378
HpyF10VI	GCNNNNNNNGC	6 cut(s)	37, 125, 289, 404, 842, 851
HpyF3I	CTNAG	4 cut(s)	258, 733, 812, 1522
HpySE526I	ACGT	2 cut(s)	249, 1374
Hsp92I	GRCGYC	1 cut(s)	107
Hsp92II	CATG	6 cut(s)	488, 800, 850, 886, 921, 1270
HspAI	GCGC	2 cut(s)	297, 398
LmnI	GCTCC	4 cut(s)	145, 241, 571, 1389
Lsp1109I	GCAGC	1 cut(s)	854
LweI	GCATC	1 cut(s)	783
MaeI	CTAG	3 cut(s)	474, 480, 1283
MaeII	ACGT	2 cut(s)	249, 1374
MaeIII	GTNAC	3 cut(s)	56, 649, 1336
MboII	GAAGA	8 cut(s)	463, 641, 653, 1063, 1085, 1190, 1236, 1538
MflI	RGATCY	3 cut(s)	1075, 1195, 1367
MhlI	GDGCHC	3 cut(s)	26, 548, 1207
MluCI	AATT	6 cut(s)	140, 314, 861, 1020, 1068, 1083
MluI	ACGCGT	1 cut(s)	363
MlyI	GAGTC	5 cut(s)	53, 55, 257, 540, 1124
MmeI	TCCRAC	1 cut(s)	989
Mph1103I	ATGCAT	2 cut(s)	798, 1268
MseI	TTAA	3 cut(s)	174, 705, 864
MslI	CAYNNNNRTG	1 cut(s)	783
MspI	CCGG	5 cut(s)	120, 132, 341, 356, 371
MspR9I	CCNGG	3 cut(s)	606, 666, 1365
Mva1269I	GAATGC	3 cut(s)	1268, 1433, 1546
MvaI	CCWGG	3 cut(s)	606, 666, 1365
MvnI	CGCG	3 cut(s)	185, 351, 365
MwoI	GCNNNNNNNGC	6 cut(s)	37, 125, 289, 404, 842, 851
NcoI	CCATGG	2 cut(s)	846, 917
NdeI	CATATG	2 cut(s)	655, 1298
NlaIII	CATG	6 cut(s)	488, 800, 850, 886, 921, 1270
NlaIV	GGNNCC	2 cut(s)	855, 915
NmeAIII	GCCGAG	1 cut(s)	56
NmuCI	GTSAC	3 cut(s)	56, 649, 1336
NruI	TCGCGA	2 cut(s)	185, 351
NsiI	ATGCAT	2 cut(s)	798, 1268
NspI	RCATGY	2 cut(s)	800, 1270
OliI	CACNNNNGTG	1 cut(s)	783
PaeI	GCATGC	2 cut(s)	800, 1270
PaqCI	CACCTGC	1 cut(s)	827
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PctI	GAATGC	3 cut(s)	1268, 1433, 1546
PfeI	GAWTC	4 cut(s)	72, 180, 412, 700
PflMI	CCANNNNNTGG	1 cut(s)	306
PkrI	GCNGC	1 cut(s)	844
Ple19I	CGATCG	1 cut(s)	189
PleI	GAGTC	5 cut(s)	53, 54, 257, 539, 1123
PpsI	GAGTC	5 cut(s)	53, 54, 257, 539, 1123
Ppu21I	YACGTR	1 cut(s)	1375
PshBI	ATTAAT	1 cut(s)	864
Psp124BI	GAGCTC	1 cut(s)	26
Psp1406I	AACGTT	1 cut(s)	249
Psp6I	CCWGG	3 cut(s)	604, 664, 1363
PspFI	CCCAGC	3 cut(s)	535, 692, 772
PspGI	CCWGG	3 cut(s)	604, 664, 1363
PspN4I	GGNNCC	2 cut(s)	855, 915
PspPI	GGNCC	4 cut(s)	439, 775, 853, 1472
PsuI	RGATCY	3 cut(s)	1075, 1195, 1367
PvuI	CGATCG	1 cut(s)	189
RgaI	GCGATCGC	1 cut(s)	189
RruI	TCGCGA	2 cut(s)	185, 351
RsaI	GTAC	5 cut(s)	269, 276, 459, 489, 1409
RsaNI	GTAC	5 cut(s)	268, 275, 458, 488, 1408
RseI	CAYNNNNRTG	1 cut(s)	783
SacI	GAGCTC	1 cut(s)	26
SaqAI	TTAA	3 cut(s)	174, 705, 864
SatI	GCNGC	1 cut(s)	843
Sau96I	GGNCC	4 cut(s)	439, 775, 853, 1472
SchI	GAGTC	5 cut(s)	53, 55, 257, 540, 1124
ScrFI	CCNGG	3 cut(s)	606, 666, 1365
SduI	GDGCHC	3 cut(s)	26, 548, 1207
SfaAI	GCGATCGC	1 cut(s)	189
SfaNI	GCATC	1 cut(s)	783
SgfI	GCGATCGC	1 cut(s)	189
SinI	GGWCC	2 cut(s)	439, 1472
SmiMI	CAYNNNNRTG	1 cut(s)	783
SmlI	CTYRAG	2 cut(s)	781, 1467
SmoI	CTYRAG	2 cut(s)	781, 1467
SphI	GCATGC	2 cut(s)	800, 1270
Sse9I	AATT	6 cut(s)	140, 314, 861, 1020, 1068, 1083
SsiI	CCGC	4 cut(s)	31, 135, 643, 1123
SspI	AATATT	1 cut(s)	1094
SspMI	CTAG	3 cut(s)	474, 480, 1283
SstI	GAGCTC	1 cut(s)	26
StyD4I	CCNGG	3 cut(s)	604, 664, 1363
StyI	CCWWGG	4 cut(s)	300, 846, 917, 1126
TaaI	ACNGT	3 cut(s)	1176, 1215, 1253
TaiI	ACGT	2 cut(s)	252, 1377
TaqI	TCGA	6 cut(s)	5, 20, 550, 1118, 1482, 1511
TaqII	GACCGA	1 cut(s)	1355
TasI	AATT	6 cut(s)	140, 314, 861, 1020, 1068, 1083
TatI	WGTACW	2 cut(s)	457, 487
TfiI	GAWTC	4 cut(s)	72, 180, 412, 700
Tru1I	TTAA	3 cut(s)	174, 705, 864
Tru9I	TTAA	3 cut(s)	174, 705, 864
TscAI	CASTG	5 cut(s)	453, 1050, 1220, 1258, 1341
TseFI	GTSAC	3 cut(s)	56, 649, 1336
TseI	GCWGC	1 cut(s)	842
Tsp45I	GTSAC	3 cut(s)	56, 649, 1336
TspDTI	ATGAA	9 cut(s)	379, 425, 573, 888, 914, 1074, 1151, 1407, 1440
TspRI	CASTG	5 cut(s)	453, 1050, 1220, 1258, 1341
Van91I	CCANNNNNTGG	1 cut(s)	306
VpaK11BI	GGWCC	2 cut(s)	439, 1472
VspI	ATTAAT	1 cut(s)	864
XceI	RCATGY	2 cut(s)	800, 1270
XmiI	GTMKAC	1 cut(s)	601
XspI	CTAG	3 cut(s)	474, 480, 1283
Zsp2I	ATGCAT	2 cut(s)	798, 1268

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.