RLG00000024403

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001825
Physical Location & Seq
Reverse (-)
35516098 .. 35520124
4027 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024403

Sequence Viewer

Length: 1140 bp
ATGGCGGAGGTGAGAGGATCGAACTCATTCCGGGACGAGATGGCCAGTCTATTGGGGGATGCAGGGATCCGATACGCCGCGGCCGATCCTATCGGAATCTCGACGCCGTCGTTTCCAGCACCGACGGCGGGTTACGTCTCCGGCGACTCGCCGGAGCAATCGGAGAGTTTGAAGGATCAGGTTAAGGGATTTGCAATGGCTTGGGGGGAGATTCTACTGGATTTGGGGAGAGGATGTAAGGACATTGTGCAGCAGAGCCTTTTAACTGAGGACTCGTACATTGTGAGGAAGCTTGAGATGCCCTGCGCCAAAGCGTCGGCGAAGTTGAGGTACTTGAATGAGTTCTTGCCGGAGGATCGCGATCCGGCTCACGCTTGGCTGGTGATTCTCTTTGTTTTCATTCTTGCACTTACAGCTGTAAGTATAAATACCGAACACGACAGCTTGGTTCGACAAGTGAAGAGAGTGCAAATGCATCCTCCCAGTGCTACTCATGTCGTACTTCCAGATGGAAGGCGATTGGCTTATCACGAGCAAGGTCTCCCAGCTAAAACAACTCGGATTTCACTGATTGCTCCTCATTCTTTTCTTTCATCACGACTTGCAGGTATTCCGGGAATTAAAATATCATTGCTTGAAGAGTTTGGTATCCGCTTGATCACATATGATCTTCCTGGCTTTGGGGAGAGTGATCCTCATCCCAGCAGGAATCTTAACTCATCTGCTTTGGATATCCTGTGTTTAGCCAATGCCATTGGTATTGAAGACAAGTTTTGGGTGCTGGGCCACTCAAGTGGAGCCCTGCATGCTTGGGCTGCTCTCAGATACATCCCTGATAAAGTTGCAGGTGCAGCTATGGTGGCCCCAATAATTAATCCCTATGAGCCTGGCATGACTAAGGAAGAGATGAAAAGAACTTGGGAACCATGGGTTTTTAGAAGGAAAACAATGTACTTCTTAGCTCGTAGGTTTCCAAAGTTTCTAAACTCTTATTATCGCCGAAGCTTCCTATCAGGAAAGCATGACAGAATTGACAAGTGGTTATCTTTCTCACTGGGGAAGAGGGTAAGTTCATTATTATTGAAATTTCATTGCCTTCTTTTTATAATTGTGAGAGAGAAGGAATCAACGCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

380

Amino Acids

42.35

Weight (kDa)

9.02

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 209 - 328 2e-13 alpha/beta hydrolase fold
Hydrolase_4 PF12146 212 - 304 2.7e-08 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 215 - 336 8.9e-10 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1106
AarI CACCTGC 1 cut(s) 836
Acc36I ACCTGC 2 cut(s) 596, 836
AccII CGCG 2 cut(s) 80, 360
AciI CCGC 5 cut(s) 5, 78, 80, 128, 652
AclWI GGATC 8 cut(s) 25, 61, 74, 80, 183, 356, 363, 686
AcoI YGGCCR 2 cut(s) 42, 81
AcsI RAATTY 1 cut(s) 1085
AcyI GRCGYC 1 cut(s) 104
AfaI GTAC 4 cut(s) 278, 332, 501, 953
AfiI CCNNNNNNNGG 2 cut(s) 128, 680
AgsI TTSAA 5 cut(s) 172, 337, 638, 764, 1084
AjnI CCWGG 2 cut(s) 673, 886
AleI CACNNNNGTG 1 cut(s) 792
AluBI AGCT 8 cut(s) 292, 416, 444, 548, 854, 962, 1005, 1136
AluI AGCT 8 cut(s) 292, 416, 444, 548, 854, 962, 1005, 1136
Alw26I GTCTC 2 cut(s) 142, 545
AlwI GGATC 8 cut(s) 25, 61, 74, 80, 183, 356, 363, 686
AoxI GGCC 4 cut(s) 42, 81, 784, 861
ApeKI GCWGC 3 cut(s) 250, 815, 851
ApoI RAATTY 1 cut(s) 1085
AseI ATTAAT 1 cut(s) 873
Asp700I GAANNNNTTC 2 cut(s) 26, 341
AspLEI GCGC 1 cut(s) 308
AspS9I GGNCC 2 cut(s) 784, 862
AsuC2I CCSGG 2 cut(s) 32, 615
AsuHPI GGTGA 2 cut(s) 22, 394
BalI TGGCCA 1 cut(s) 44
BamHI GGATCC 1 cut(s) 66
BanII GRGCYC 1 cut(s) 802
BarI GAAGNNNNNNTAC 2 cut(s) 314, 346
BauI CACGAG 1 cut(s) 530
BbsI GAAGAC 1 cut(s) 771
BbvI GCAGC 3 cut(s) 262, 802, 863
BccI CCATC 2 cut(s) 34, 503
BceAI ACGGC 2 cut(s) 91, 141
BciT130I CCWGG 2 cut(s) 675, 888
BciVI GTATCC 1 cut(s) 659
BclI TGATCA 1 cut(s) 657
BcnI CCSGG 2 cut(s) 32, 615
BcoDI GTCTC 2 cut(s) 142, 545
BfuAI ACCTGC 2 cut(s) 596, 836
BfuI GTATCC 1 cut(s) 659
BisI GCNGC 5 cut(s) 78, 81, 251, 816, 852
BlsI GCNGC 5 cut(s) 79, 82, 252, 817, 853
Bme1390I CCNGG 4 cut(s) 32, 615, 675, 888
BmgT120I GGNCC 2 cut(s) 784, 862
BmiI GGNNCC 4 cut(s) 68, 799, 864, 924
BmrFI CCNGG 4 cut(s) 32, 615, 675, 888
BmrI ACTGGG 2 cut(s) 477, 1064
BmsI GCATC 3 cut(s) 49, 288, 484
BmuI ACTGGG 2 cut(s) 477, 1064
BpiI GAAGAC 1 cut(s) 771
BplI GAGNNNNNCTC 2 cut(s) 679, 711
BpuEI CTTGAG 2 cut(s) 314, 775
BpuMI CCSGG 2 cut(s) 32, 615
BsaBI GATNNNNATC 2 cut(s) 360, 696
BsaHI GRCGYC 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 545
BsaJI CCNNGG 2 cut(s) 78, 926
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 680
Bse1I ACTGG 4 cut(s) 45, 222, 483, 1059
Bse3DI GCAATG 3 cut(s) 201, 629, 1090
Bse8I GATNNNNATC 2 cut(s) 360, 696
BseBI CCWGG 2 cut(s) 675, 888
BseDI CCNNGG 2 cut(s) 78, 926
BseGI GGATG 5 cut(s) 64, 239, 475, 697, 828
BseJI GATNNNNATC 2 cut(s) 360, 696
BseLI CCNNNNNNNGG 2 cut(s) 128, 680
BseMI GCAATG 3 cut(s) 201, 629, 1090
BseMII CTCAG 2 cut(s) 258, 835
BseNI ACTGG 4 cut(s) 45, 222, 483, 1059
BseRI GAGGAG 1 cut(s) 567
BseX3I CGGCCG 1 cut(s) 81
BseXI GCAGC 3 cut(s) 262, 802, 863
BseYI CCCAGC 3 cut(s) 544, 701, 781
BsgI GTGCAG 2 cut(s) 269, 870
Bsh1236I CGCG 2 cut(s) 80, 360
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 4 cut(s) 44, 83, 786, 863
BsiEI CGRYCG 1 cut(s) 84
BsiSI CCGG 6 cut(s) 31, 141, 152, 350, 365, 614
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 2 cut(s) 128, 680
BsmAI GTCTC 2 cut(s) 142, 545
BsmBI CGTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 47
BsnI GGCC 4 cut(s) 44, 83, 786, 863
Bso31I GGTCTC 1 cut(s) 545
Bsp1286I GDGCHC 1 cut(s) 802
Bsp143I GATC 9 cut(s) 17, 66, 85, 175, 355, 361, 657, 667, 691
Bsp19I CCATGG 1 cut(s) 926
Bsp68I TCGCGA 1 cut(s) 360
BspACI CCGC 5 cut(s) 5, 78, 80, 128, 652
BspANI GGCC 4 cut(s) 44, 83, 786, 863
BspCNI CTCAG 2 cut(s) 259, 834
BspFNI CGCG 2 cut(s) 80, 360
BspLI GGNNCC 4 cut(s) 68, 799, 864, 924
BspMI ACCTGC 2 cut(s) 596, 836
BspPI GGATC 8 cut(s) 25, 61, 74, 80, 183, 356, 363, 686
BspTNI GGTCTC 1 cut(s) 545
BsrDI GCAATG 3 cut(s) 201, 629, 1090
BsrI ACTGG 4 cut(s) 45, 222, 483, 1059
BssECI CCNNGG 2 cut(s) 78, 926
BssMI GATC 9 cut(s) 17, 66, 85, 175, 355, 361, 657, 667, 691
BssNI GRCGYC 1 cut(s) 104
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 926
Bst2BI CACGAG 1 cut(s) 530
Bst2UI CCWGG 2 cut(s) 675, 888
Bst6I CTCTTC 4 cut(s) 455, 633, 897, 1055
BstACI GRCGYC 1 cut(s) 104
BstC8I GCNNGC 2 cut(s) 807, 1134
BstDEI CTNAG 4 cut(s) 267, 821, 897, 958
BstDSI CCRYGG 2 cut(s) 78, 926
BstF5I GGATG 5 cut(s) 64, 239, 475, 697, 828
BstFNI CGCG 2 cut(s) 80, 360
BstHHI GCGC 1 cut(s) 308
BstKTI GATC 9 cut(s) 20, 69, 88, 178, 358, 364, 660, 670, 694
BstMAI GTCTC 2 cut(s) 142, 545
BstMBI GATC 9 cut(s) 17, 66, 85, 175, 355, 361, 657, 667, 691
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 7 cut(s) 125, 298, 413, 806, 815, 851, 860
BstNI CCWGG 2 cut(s) 675, 888
BstNSI RCATGY 1 cut(s) 809
BstSCI CCNGG 4 cut(s) 30, 613, 673, 886
BstUI CGCG 2 cut(s) 80, 360
BstV1I GCAGC 3 cut(s) 262, 802, 863
BstV2I GAAGAC 1 cut(s) 771
BstX2I RGATCY 1 cut(s) 66
BstXI CCANNNNNNTGG 2 cut(s) 52, 794
BstYI RGATCY 1 cut(s) 66
BstZI CGGCCG 1 cut(s) 81
BsuI GTATCC 1 cut(s) 659
BsuRI GGCC 4 cut(s) 44, 83, 786, 863
BtgI CCRYGG 2 cut(s) 78, 926
BtsCI GGATG 5 cut(s) 64, 239, 475, 697, 828
BtsIMutI CAGTG 3 cut(s) 490, 566, 1052
BtuMI TCGCGA 1 cut(s) 360
BveI ACCTGC 2 cut(s) 596, 836
Cac8I GCNNGC 2 cut(s) 807, 1134
CfoI GCGC 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 784, 862
Cfr42I CCGCGG 1 cut(s) 81
CseI GACGC 2 cut(s) 112, 303
Csp6I GTAC 4 cut(s) 277, 331, 500, 952
CviAII CATG 5 cut(s) 494, 806, 892, 927, 1022
CviQI GTAC 4 cut(s) 277, 331, 500, 952
DdeI CTNAG 4 cut(s) 267, 821, 897, 958
DpnI GATC 9 cut(s) 19, 68, 87, 177, 357, 363, 659, 669, 693
DpnII GATC 9 cut(s) 17, 66, 85, 175, 355, 361, 657, 667, 691
EaeI YGGCCR 2 cut(s) 42, 81
EagI CGGCCG 1 cut(s) 81
Eam1104I CTCTTC 4 cut(s) 455, 633, 897, 1055
EarI CTCTTC 4 cut(s) 455, 633, 897, 1055
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 926
Eco24I GRGCYC 1 cut(s) 802
Eco31I GGTCTC 1 cut(s) 545
Eco32I GATATC 1 cut(s) 733
Eco52I CGGCCG 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 673, 886
EcoRV GATATC 1 cut(s) 733
EcoT14I CCWWGG 1 cut(s) 926
EcoT22I ATGCAT 1 cut(s) 477
EcoT38I GRGCYC 1 cut(s) 802
ErhI CCWWGG 1 cut(s) 926
Esp3I CGTCTC 1 cut(s) 142
FaeI CATG 5 cut(s) 497, 809, 895, 930, 1025
FaqI GGGAC 1 cut(s) 47
FatI CATG 5 cut(s) 493, 805, 891, 926, 1021
FauI CCCGC 1 cut(s) 121
FauNDI CATATG 1 cut(s) 664
FbaI TGATCA 1 cut(s) 657
Fnu4HI GCNGC 5 cut(s) 78, 81, 251, 816, 852
FokI GGATG 5 cut(s) 71, 246, 462, 684, 815
FriOI GRGCYC 1 cut(s) 802
Fsp4HI GCNGC 5 cut(s) 78, 81, 251, 816, 852
GlaI GCGC 1 cut(s) 307
GluI GCNGC 5 cut(s) 78, 81, 251, 816, 852
GsaI CCCAGC 3 cut(s) 548, 705, 785
HaeIII GGCC 4 cut(s) 44, 83, 786, 863
HapII CCGG 6 cut(s) 31, 141, 152, 350, 365, 614
HgaI GACGC 2 cut(s) 112, 303
HhaI GCGC 1 cut(s) 308
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 5 cut(s) 497, 809, 895, 930, 1025
Hin6I GCGC 1 cut(s) 306
HinP1I GCGC 1 cut(s) 306
HindIII AAGCTT 2 cut(s) 290, 1003
HinfI GANTC 7 cut(s) 96, 146, 211, 272, 385, 709, 1124
HpaII CCGG 6 cut(s) 31, 141, 152, 350, 365, 614
HphI GGTGA 2 cut(s) 22, 394
Hpy188I TCNGA 5 cut(s) 71, 95, 163, 561, 824
Hpy188III TCNNGA 6 cut(s) 100, 359, 506, 530, 597, 1014
Hpy99I CGWCG 4 cut(s) 106, 112, 127, 319
HpyAV CCTTC 5 cut(s) 166, 507, 933, 1106, 1114
HpyCH4IV ACGT 1 cut(s) 135
HpyF10VI GCNNNNNNNGC 7 cut(s) 125, 298, 413, 806, 815, 851, 860
HpyF3I CTNAG 4 cut(s) 267, 821, 897, 958
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 5 cut(s) 497, 809, 895, 930, 1025
HspAI GCGC 1 cut(s) 306
Ksp22I TGATCA 1 cut(s) 657
KspI CCGCGG 1 cut(s) 81
Kzo9I GATC 9 cut(s) 17, 66, 85, 175, 355, 361, 657, 667, 691
LmnI GCTCC 3 cut(s) 154, 580, 797
Lsp1109I GCAGC 3 cut(s) 262, 802, 863
LweI GCATC 3 cut(s) 49, 288, 484
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 131
MalI GATC 9 cut(s) 19, 68, 87, 177, 357, 363, 659, 669, 693
MboI GATC 9 cut(s) 17, 66, 85, 175, 355, 361, 657, 667, 691
MboII GAAGA 6 cut(s) 472, 650, 662, 776, 914, 1072
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 802
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 5 cut(s) 618, 870, 1029, 1085, 1107
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 2 cut(s) 140, 266
Mox20I TGGCCA 1 cut(s) 44
Mph1103I ATGCAT 1 cut(s) 477
MroXI GAANNNNTTC 2 cut(s) 26, 341
MscI TGGCCA 1 cut(s) 44
MseI TTAA 5 cut(s) 183, 263, 621, 714, 873
MslI CAYNNNNRTG 1 cut(s) 792
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 2 cut(s) 80, 416
MspI CCGG 6 cut(s) 31, 141, 152, 350, 365, 614
MspR9I CCNGG 4 cut(s) 32, 615, 675, 888
MvaI CCWGG 2 cut(s) 675, 888
MvnI CGCG 2 cut(s) 80, 360
MwoI GCNNNNNNNGC 7 cut(s) 125, 298, 413, 806, 815, 851, 860
NciI CCSGG 2 cut(s) 32, 615
NcoI CCATGG 1 cut(s) 926
NdeI CATATG 1 cut(s) 664
NdeII GATC 9 cut(s) 17, 66, 85, 175, 355, 361, 657, 667, 691
NlaIII CATG 5 cut(s) 497, 809, 895, 930, 1025
NlaIV GGNNCC 4 cut(s) 68, 799, 864, 924
NruI TCGCGA 1 cut(s) 360
NsiI ATGCAT 1 cut(s) 477
NspI RCATGY 1 cut(s) 809
OliI CACNNNNGTG 1 cut(s) 792
PaeI GCATGC 1 cut(s) 809
PaqCI CACCTGC 1 cut(s) 836
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 107, 141
PdmI GAANNNNTTC 2 cut(s) 26, 341
PfeI GAWTC 5 cut(s) 96, 211, 385, 709, 1124
PflFI GACNNNGTC 1 cut(s) 106
PfoI TCCNGGA 2 cut(s) 30, 613
PkrI GCNGC 5 cut(s) 79, 82, 252, 817, 853
PleI GAGTC 2 cut(s) 140, 266
PpsI GAGTC 2 cut(s) 140, 266
PshBI ATTAAT 1 cut(s) 873
PsiI TTATAA 1 cut(s) 1106
Psp6I CCWGG 2 cut(s) 673, 886
PspFI CCCAGC 3 cut(s) 544, 701, 781
PspGI CCWGG 2 cut(s) 673, 886
PspN4I GGNNCC 4 cut(s) 68, 799, 864, 924
PspPI GGNCC 2 cut(s) 784, 862
PsuI RGATCY 1 cut(s) 66
PsyI GACNNNGTC 1 cut(s) 106
PvuII CAGCTG 1 cut(s) 416
RruI TCGCGA 1 cut(s) 360
RsaI GTAC 4 cut(s) 278, 332, 501, 953
RsaNI GTAC 4 cut(s) 277, 331, 500, 952
RseI CAYNNNNRTG 1 cut(s) 792
SacII CCGCGG 1 cut(s) 81
SaqAI TTAA 5 cut(s) 183, 263, 621, 714, 873
SatI GCNGC 5 cut(s) 78, 81, 251, 816, 852
Sau3AI GATC 9 cut(s) 17, 66, 85, 175, 355, 361, 657, 667, 691
Sau96I GGNCC 2 cut(s) 784, 862
SchI GAGTC 2 cut(s) 140, 266
ScrFI CCNGG 4 cut(s) 32, 615, 675, 888
SduI GDGCHC 1 cut(s) 802
SfaNI GCATC 3 cut(s) 49, 288, 484
Sfr303I CCGCGG 1 cut(s) 81
SgrBI CCGCGG 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 792
SmlI CTYRAG 2 cut(s) 293, 790
SmoI CTYRAG 2 cut(s) 293, 790
SphI GCATGC 1 cut(s) 809
Sse9I AATT 5 cut(s) 618, 870, 1029, 1085, 1107
SsiI CCGC 5 cut(s) 5, 78, 80, 128, 652
StyD4I CCNGG 4 cut(s) 30, 613, 673, 886
StyI CCWWGG 1 cut(s) 926
TaiI ACGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 20, 101, 451
TasI AATT 5 cut(s) 618, 870, 1029, 1085, 1107
TatI WGTACW 1 cut(s) 951
TauI GCSGC 2 cut(s) 80, 83
TfiI GAWTC 5 cut(s) 96, 211, 385, 709, 1124
Tru1I TTAA 5 cut(s) 183, 263, 621, 714, 873
Tru9I TTAA 5 cut(s) 183, 263, 621, 714, 873
TscAI CASTG 3 cut(s) 490, 573, 1059
TseI GCWGC 3 cut(s) 250, 815, 851
TspDTI ATGAA 5 cut(s) 388, 582, 923, 1062, 1079
TspRI CASTG 3 cut(s) 490, 573, 1059
Tth111I GACNNNGTC 1 cut(s) 106
VspI ATTAAT 1 cut(s) 873
XapI RAATTY 1 cut(s) 1085
XceI RCATGY 1 cut(s) 809
XmnI GAANNNNTTC 2 cut(s) 26, 341
Zsp2I ATGCAT 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.