Alpha/beta hydrolase family
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G09690 AT3G09690 AT5G02970 |
| fragaria_vesca | FvH4_3g33440 FvH4_3g33440 FvH4_3g33440 FvH4_3g33440 FvH4_6g15410 FvH4_6g15410 FvH4_6g15410 FvH4_6g15410 FvH4_6g15410 |
| malus_domestica | MD03G1111700.v1.1 MD04G1103800.v1.1 MD11G1126200.v1.1 |
| prunus_persica | Prupe.6G094600_v2.0.a1 Prupe.6G240800_v2.0.a1 Prupe.6G240800_v2.0.a1 Prupe.6G240800_v2.0.a1 Prupe.6G240800_v2.0.a1 |
| pyrus_communis | pycom04g09900 pycom11g10650 pycom12g12240 |
| rosa_chinensis | RchiOBHm_Chr3g0468891 RchiOBHm_Chr5g0060781 |
| rosa_laevigata | RLG00000024403 RLG00000035400 |
| rosa_multiflora | Rmu_co8389401.1_g000001 Rmu_sc0000727.1_g000032 Rmu_sc0001639.1_g000013 Rmu_sc0003298.1_g000004 Rmu_sc0007143.1_g000009 |
| rosa_roxburghii | Rroxscaffold_1G00019940 Rroxscaffold_6G00412480 |
| rosa_rugosa | Rorug03G0097200 Rorug03G0097300 Rorug03G0097400 Rorug05G0334000 |
| rosa_samantha | Rh3AG147000 Rh3BG169500 Rh3DG205600 Rh5AG396700 Rh5BG408700 Rh5CG433400 Rh5DG424000 |
| rosa_wichuraiana | Rw3G013830 Rw5G037400 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1785 |
| Acc36I | ACCTGC | 1 cut(s) | 1002 |
| Acc65I | GGTACC | 1 cut(s) | 1758 |
| AccB1I | GGYRCC | 2 cut(s) | 1561, 1758 |
| AccBSI | CCGCTC | 1 cut(s) | 754 |
| AccII | CGCG | 1 cut(s) | 1352 |
| AccIII | TCCGGA | 2 cut(s) | 523, 1960 |
| AciI | CCGC | 7 cut(s) | 429, 544, 754, 767, 1350, 1991, 2142 |
| AclWI | GGATC | 5 cut(s) | 174, 250, 1280, 1293, 1952 |
| AcoI | YGGCCR | 3 cut(s) | 768, 838, 1551 |
| AcsI | RAATTY | 3 cut(s) | 933, 1541, 2101 |
| AcuI | CTGAAG | 2 cut(s) | 504, 1674 |
| AdeI | CACNNNGTG | 1 cut(s) | 126 |
| AfaI | GTAC | 4 cut(s) | 297, 327, 1760, 2147 |
| AfeI | AGCGCT | 1 cut(s) | 481 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 530, 1090, 1967 |
| AflIII | ACRYGT | 2 cut(s) | 829, 2190 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1182 |
| AjnI | CCWGG | 2 cut(s) | 1897, 2241 |
| AleI | CACNNNNGTG | 1 cut(s) | 2297 |
| Alw21I | GWGCWC | 1 cut(s) | 2304 |
| Alw26I | GTCTC | 2 cut(s) | 87, 373 |
| Alw44I | GTGCAC | 1 cut(s) | 2300 |
| AlwI | GGATC | 5 cut(s) | 174, 250, 1280, 1293, 1952 |
| Aor13HI | TCCGGA | 2 cut(s) | 523, 1960 |
| Aor51HI | AGCGCT | 1 cut(s) | 481 |
| AoxI | GGCC | 5 cut(s) | 768, 838, 1436, 1551, 1860 |
| ApaLI | GTGCAC | 1 cut(s) | 2300 |
| ApeKI | GCWGC | 3 cut(s) | 812, 849, 1915 |
| ApoI | RAATTY | 3 cut(s) | 933, 1541, 2101 |
| Asp718I | GGTACC | 1 cut(s) | 1758 |
| AspA2I | CCTAGG | 1 cut(s) | 1028 |
| AspLEI | GCGC | 2 cut(s) | 482, 1354 |
| AspS9I | GGNCC | 3 cut(s) | 893, 1861, 1987 |
| AsuHPI | GGTGA | 4 cut(s) | 368, 815, 1616, 1943 |
| AvaII | GGWCC | 2 cut(s) | 893, 1987 |
| AvrII | CCTAGG | 1 cut(s) | 1028 |
| BaeGI | GKGCMC | 1 cut(s) | 2304 |
| BalI | TGGCCA | 2 cut(s) | 840, 1553 |
| BamHI | GGATCC | 1 cut(s) | 1285 |
| BanI | GGYRCC | 2 cut(s) | 1561, 1758 |
| BauI | CACGAG | 1 cut(s) | 2303 |
| Bbv12I | GWGCWC | 1 cut(s) | 2304 |
| BbvCI | CCTCAGC | 1 cut(s) | 1212 |
| BbvI | GCAGC | 3 cut(s) | 799, 836, 1902 |
| BccI | CCATC | 7 cut(s) | 389, 917, 977, 1316, 1349, 1573, 1616 |
| BciT130I | CCWGG | 2 cut(s) | 1899, 2243 |
| BciVI | GTATCC | 1 cut(s) | 2029 |
| BclI | TGATCA | 2 cut(s) | 1933, 2254 |
| BcoDI | GTCTC | 2 cut(s) | 87, 373 |
| BfaI | CTAG | 7 cut(s) | 90, 695, 705, 1029, 1316, 1448, 2127 |
| BfmI | CTRYAG | 1 cut(s) | 1461 |
| BfoI | RGCGCY | 1 cut(s) | 483 |
| BfuAI | ACCTGC | 1 cut(s) | 1002 |
| BfuI | GTATCC | 1 cut(s) | 2029 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1559 |
| BglII | AGATCT | 1 cut(s) | 32 |
| BisI | GCNGC | 5 cut(s) | 768, 813, 850, 1350, 1916 |
| BlnI | CCTAGG | 1 cut(s) | 1028 |
| BlpI | GCTNAGC | 1 cut(s) | 476 |
| BlsI | GCNGC | 5 cut(s) | 769, 814, 851, 1351, 1917 |
| Bme1390I | CCNGG | 2 cut(s) | 1899, 2243 |
| Bme18I | GGWCC | 2 cut(s) | 893, 1987 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1182 |
| BmgT120I | GGNCC | 3 cut(s) | 893, 1861, 1987 |
| BmiI | GGNNCC | 6 cut(s) | 1140, 1287, 1563, 1650, 1760, 1989 |
| BmrFI | CCNGG | 2 cut(s) | 1899, 2243 |
| BmsI | GCATC | 2 cut(s) | 1170, 1600 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1212 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 476 |
| BpuEI | CTTGAG | 3 cut(s) | 861, 1085, 1376 |
| BsaBI | GATNNNNATC | 3 cut(s) | 1130, 2027, 2253 |
| BsaI | GGTCTC | 1 cut(s) | 87 |
| BsaJI | CCNNGG | 5 cut(s) | 841, 1028, 1479, 1898, 2241 |
| BsaWI | WCCGGW | 2 cut(s) | 523, 1960 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 546, 576, 2129, 2159, 2212, 2242 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 530, 1090, 1967 |
| Bse1I | ACTGG | 1 cut(s) | 1735 |
| Bse3DI | GCAATG | 1 cut(s) | 1740 |
| Bse8I | GATNNNNATC | 3 cut(s) | 1130, 2027, 2253 |
| BseAI | TCCGGA | 2 cut(s) | 523, 1960 |
| BseBI | CCWGG | 2 cut(s) | 1899, 2243 |
| BseDI | CCNNGG | 5 cut(s) | 841, 1028, 1479, 1898, 2241 |
| BseGI | GGATG | 7 cut(s) | 370, 400, 415, 928, 1327, 1615, 2028 |
| BseJI | GATNNNNATC | 3 cut(s) | 1130, 2027, 2253 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 530, 1090, 1967 |
| BseMI | GCAATG | 1 cut(s) | 1740 |
| BseMII | CTCAG | 3 cut(s) | 929, 1203, 1941 |
| BseNI | ACTGG | 1 cut(s) | 1735 |
| BseSI | GKGCMC | 1 cut(s) | 2304 |
| BseXI | GCAGC | 3 cut(s) | 799, 836, 1902 |
| BsgI | GTGCAG | 2 cut(s) | 135, 656 |
| Bsh1236I | CGCG | 1 cut(s) | 1352 |
| BshFI | GGCC | 5 cut(s) | 770, 840, 1438, 1553, 1862 |
| BshNI | GGYRCC | 2 cut(s) | 1561, 1758 |
| BsiHKAI | GWGCWC | 1 cut(s) | 2304 |
| BsiSI | CCGG | 3 cut(s) | 524, 1142, 1961 |
| BslFI | GGGAC | 2 cut(s) | 532, 1003 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 530, 1090, 1967 |
| BsmAI | GTCTC | 2 cut(s) | 87, 373 |
| BsmBI | CGTCTC | 1 cut(s) | 373 |
| BsmFI | GGGAC | 2 cut(s) | 532, 1003 |
| BsmI | GAATGC | 2 cut(s) | 1613, 2112 |
| BsnI | GGCC | 5 cut(s) | 770, 840, 1438, 1553, 1862 |
| Bso31I | GGTCTC | 1 cut(s) | 87 |
| Bsp1286I | GDGCHC | 1 cut(s) | 2304 |
| Bsp13I | TCCGGA | 2 cut(s) | 523, 1960 |
| Bsp1407I | TGTACA | 1 cut(s) | 295 |
| Bsp143I | GATC | 7 cut(s) | 32, 166, 242, 1285, 1933, 1957, 2254 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 476 |
| Bsp19I | CCATGG | 1 cut(s) | 841 |
| BspACI | CCGC | 7 cut(s) | 429, 544, 754, 767, 1350, 1991, 2142 |
| BspANI | GGCC | 5 cut(s) | 770, 840, 1438, 1553, 1862 |
| BspCNI | CTCAG | 3 cut(s) | 930, 1204, 1940 |
| BspEI | TCCGGA | 2 cut(s) | 523, 1960 |
| BspFNI | CGCG | 1 cut(s) | 1352 |
| BspHI | TCATGA | 3 cut(s) | 37, 1303, 2309 |
| BspLI | GGNNCC | 6 cut(s) | 1140, 1287, 1563, 1650, 1760, 1989 |
| BspMAI | CTGCAG | 1 cut(s) | 1465 |
| BspMI | ACCTGC | 1 cut(s) | 1002 |
| BspPI | GGATC | 5 cut(s) | 174, 250, 1280, 1293, 1952 |
| BspT107I | GGYRCC | 2 cut(s) | 1561, 1758 |
| BspTNI | GGTCTC | 1 cut(s) | 87 |
| BsrBI | CCGCTC | 1 cut(s) | 754 |
| BsrDI | GCAATG | 1 cut(s) | 1740 |
| BsrGI | TGTACA | 1 cut(s) | 295 |
| BsrI | ACTGG | 1 cut(s) | 1735 |
| BssECI | CCNNGG | 5 cut(s) | 841, 1028, 1479, 1898, 2241 |
| BssMI | GATC | 7 cut(s) | 32, 166, 242, 1285, 1933, 1957, 2254 |
| BssSI | CACGAG | 1 cut(s) | 2303 |
| BssT1I | CCWWGG | 3 cut(s) | 841, 1028, 1479 |
| Bst2BI | CACGAG | 1 cut(s) | 2303 |
| Bst2UI | CCWGG | 2 cut(s) | 1899, 2243 |
| Bst4CI | ACNGT | 7 cut(s) | 104, 467, 622, 1085, 1270, 1405, 1855 |
| Bst6I | CTCTTC | 2 cut(s) | 532, 1518 |
| BstAUI | TGTACA | 1 cut(s) | 295 |
| BstC8I | GCNNGC | 6 cut(s) | 21, 756, 1413, 1575, 1714, 2110 |
| BstDEI | CTNAG | 5 cut(s) | 476, 629, 938, 1212, 1927 |
| BstDSI | CCRYGG | 1 cut(s) | 841 |
| BstF5I | GGATG | 7 cut(s) | 370, 400, 415, 928, 1327, 1615, 2028 |
| BstFNI | CGCG | 1 cut(s) | 1352 |
| BstH2I | RGCGCY | 1 cut(s) | 483 |
| BstHHI | GCGC | 2 cut(s) | 482, 1354 |
| BstKTI | GATC | 7 cut(s) | 35, 169, 245, 1288, 1936, 1960, 2257 |
| BstMAI | GTCTC | 2 cut(s) | 87, 373 |
| BstMBI | GATC | 7 cut(s) | 32, 166, 242, 1285, 1933, 1957, 2254 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 428, 764, 776, 846, 1559 |
| BstNI | CCWGG | 2 cut(s) | 1899, 2243 |
| BstNSI | RCATGY | 3 cut(s) | 23, 833, 2194 |
| BstSCI | CCNGG | 2 cut(s) | 1897, 2241 |
| BstSFI | CTRYAG | 1 cut(s) | 1461 |
| BstSLI | GKGCMC | 1 cut(s) | 2304 |
| BstUI | CGCG | 1 cut(s) | 1352 |
| BstV1I | GCAGC | 3 cut(s) | 799, 836, 1902 |
| BstX2I | RGATCY | 2 cut(s) | 32, 1285 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1273 |
| BstYI | RGATCY | 2 cut(s) | 32, 1285 |
| BsuI | GTATCC | 1 cut(s) | 2029 |
| BsuRI | GGCC | 5 cut(s) | 770, 840, 1438, 1553, 1862 |
| BtgI | CCRYGG | 1 cut(s) | 841 |
| BtsCI | GGATG | 7 cut(s) | 370, 400, 415, 928, 1327, 1615, 2028 |
| BtsIMutI | CAGTG | 3 cut(s) | 472, 1266, 1410 |
| BveI | ACCTGC | 1 cut(s) | 1002 |
| Cac8I | GCNNGC | 6 cut(s) | 21, 756, 1413, 1575, 1714, 2110 |
| CciI | TCATGA | 3 cut(s) | 37, 1303, 2309 |
| CfoI | GCGC | 2 cut(s) | 482, 1354 |
| Cfr13I | GGNCC | 3 cut(s) | 893, 1861, 1987 |
| CseI | GACGC | 2 cut(s) | 1172, 2010 |
| Csp6I | GTAC | 4 cut(s) | 296, 326, 1759, 2146 |
| CviQI | GTAC | 4 cut(s) | 296, 326, 1759, 2146 |
| DdeI | CTNAG | 5 cut(s) | 476, 629, 938, 1212, 1927 |
| DpnI | GATC | 7 cut(s) | 34, 168, 244, 1287, 1935, 1959, 2256 |
| DpnII | GATC | 7 cut(s) | 32, 166, 242, 1285, 1933, 1957, 2254 |
| DraIII | CACNNNGTG | 1 cut(s) | 126 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1182 |
| EaeI | YGGCCR | 3 cut(s) | 768, 838, 1551 |
| Eam1104I | CTCTTC | 2 cut(s) | 532, 1518 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1182 |
| EarI | CTCTTC | 2 cut(s) | 532, 1518 |
| Eco130I | CCWWGG | 3 cut(s) | 841, 1028, 1479 |
| Eco31I | GGTCTC | 1 cut(s) | 87 |
| Eco32I | GATATC | 2 cut(s) | 1132, 2173 |
| Eco47I | GGWCC | 2 cut(s) | 893, 1987 |
| Eco47III | AGCGCT | 1 cut(s) | 481 |
| Eco57I | CTGAAG | 2 cut(s) | 504, 1674 |
| EcoRII | CCWGG | 2 cut(s) | 1897, 2241 |
| EcoRV | GATATC | 2 cut(s) | 1132, 2173 |
| EcoT14I | CCWWGG | 3 cut(s) | 841, 1028, 1479 |
| EcoT22I | ATGCAT | 1 cut(s) | 1615 |
| ErhI | CCWWGG | 3 cut(s) | 841, 1028, 1479 |
| Esp3I | CGTCTC | 1 cut(s) | 373 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 155, 187, 1866, 1898 |
| FaqI | GGGAC | 2 cut(s) | 532, 1003 |
| FauI | CCCGC | 4 cut(s) | 537, 747, 1998, 2135 |
| FauNDI | CATATG | 1 cut(s) | 2296 |
| FbaI | TGATCA | 2 cut(s) | 1933, 2254 |
| Fnu4HI | GCNGC | 5 cut(s) | 768, 813, 850, 1350, 1916 |
| FokI | GGATG | 7 cut(s) | 377, 407, 422, 935, 1334, 1622, 2035 |
| Fsp4HI | GCNGC | 5 cut(s) | 768, 813, 850, 1350, 1916 |
| FspBI | CTAG | 7 cut(s) | 90, 695, 705, 1029, 1316, 1448, 2127 |
| GlaI | GCGC | 2 cut(s) | 481, 1353 |
| GluI | GCNGC | 5 cut(s) | 768, 813, 850, 1350, 1916 |
| HaeII | RGCGCY | 1 cut(s) | 483 |
| HaeIII | GGCC | 5 cut(s) | 770, 840, 1438, 1553, 1862 |
| HapII | CCGG | 3 cut(s) | 524, 1142, 1961 |
| HgaI | GACGC | 2 cut(s) | 1172, 2010 |
| HhaI | GCGC | 2 cut(s) | 482, 1354 |
| Hin6I | GCGC | 2 cut(s) | 480, 1352 |
| HinP1I | GCGC | 2 cut(s) | 480, 1352 |
| HincII | GTYRAC | 5 cut(s) | 1081, 1187, 1813, 1947, 2290 |
| HindII | GTYRAC | 5 cut(s) | 1081, 1187, 1813, 1947, 2290 |
| HindIII | AAGCTT | 1 cut(s) | 458 |
| HinfI | GANTC | 8 cut(s) | 236, 269, 284, 290, 416, 520, 1529, 2306 |
| HpaII | CCGG | 3 cut(s) | 524, 1142, 1961 |
| HphI | GGTGA | 4 cut(s) | 368, 815, 1616, 1943 |
| Hpy166II | GTNNAC | 7 cut(s) | 913, 1081, 1187, 1813, 1947, 2290, 2302 |
| Hpy188I | TCNGA | 8 cut(s) | 274, 289, 626, 652, 939, 1534, 2062, 2082 |
| Hpy8I | GTNNAC | 7 cut(s) | 913, 1081, 1187, 1813, 1947, 2290, 2302 |
| HpyAV | CCTTC | 6 cut(s) | 347, 479, 1299, 1771, 1827, 2214 |
| HpyCH4III | ACNGT | 7 cut(s) | 104, 467, 622, 1085, 1270, 1405, 1855 |
| HpyCH4IV | ACGT | 2 cut(s) | 595, 1206 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 428, 764, 776, 846, 1559 |
| HpyF3I | CTNAG | 5 cut(s) | 476, 629, 938, 1212, 1927 |
| HpySE526I | ACGT | 2 cut(s) | 595, 1206 |
| HspAI | GCGC | 2 cut(s) | 480, 1352 |
| Kpn2I | TCCGGA | 2 cut(s) | 523, 1960 |
| KpnI | GGTACC | 1 cut(s) | 1762 |
| Ksp22I | TGATCA | 2 cut(s) | 1933, 2254 |
| Kzo9I | GATC | 7 cut(s) | 32, 166, 242, 1285, 1933, 1957, 2254 |
| LmnI | GCTCC | 6 cut(s) | 370, 526, 751, 926, 1138, 1648 |
| Lsp1109I | GCAGC | 3 cut(s) | 799, 836, 1902 |
| LweI | GCATC | 2 cut(s) | 1170, 1600 |
| MaeI | CTAG | 7 cut(s) | 90, 695, 705, 1029, 1316, 1448, 2127 |
| MaeII | ACGT | 2 cut(s) | 595, 1206 |
| MaeIII | GTNAC | 4 cut(s) | 302, 562, 1726, 1885 |
| MalI | GATC | 7 cut(s) | 34, 168, 244, 1287, 1935, 1959, 2256 |
| MbiI | CCGCTC | 1 cut(s) | 754 |
| MboI | GATC | 7 cut(s) | 32, 166, 242, 1285, 1933, 1957, 2254 |
| MfeI | CAATTG | 1 cut(s) | 279 |
| MflI | RGATCY | 2 cut(s) | 32, 1285 |
| MhlI | GDGCHC | 1 cut(s) | 2304 |
| MlsI | TGGCCA | 2 cut(s) | 840, 1553 |
| MluCI | AATT | 8 cut(s) | 279, 329, 738, 933, 1395, 1541, 1556, 2101 |
| MluNI | TGGCCA | 2 cut(s) | 840, 1553 |
| MlyI | GAGTC | 5 cut(s) | 245, 284, 425, 514, 1523 |
| MmeI | TCCRAC | 4 cut(s) | 330, 879, 996, 2060 |
| Mox20I | TGGCCA | 2 cut(s) | 840, 1553 |
| Mph1103I | ATGCAT | 1 cut(s) | 1615 |
| MroI | TCCGGA | 2 cut(s) | 523, 1960 |
| MscI | TGGCCA | 2 cut(s) | 840, 1553 |
| MseI | TTAA | 4 cut(s) | 189, 503, 1002, 1196 |
| MslI | CAYNNNNRTG | 4 cut(s) | 828, 1271, 1745, 2297 |
| Msp20I | TGGCCA | 2 cut(s) | 840, 1553 |
| MspA1I | CMGCKG | 2 cut(s) | 431, 1993 |
| MspI | CCGG | 3 cut(s) | 524, 1142, 1961 |
| MspR9I | CCNGG | 2 cut(s) | 1899, 2243 |
| MunI | CAATTG | 1 cut(s) | 279 |
| Mva1269I | GAATGC | 2 cut(s) | 1613, 2112 |
| MvaI | CCWGG | 2 cut(s) | 1899, 2243 |
| MvnI | CGCG | 1 cut(s) | 1352 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 428, 764, 776, 846, 1559 |
| NcoI | CCATGG | 1 cut(s) | 841 |
| NdeI | CATATG | 1 cut(s) | 2296 |
| NdeII | GATC | 7 cut(s) | 32, 166, 242, 1285, 1933, 1957, 2254 |
| NlaIV | GGNNCC | 6 cut(s) | 1140, 1287, 1563, 1650, 1760, 1989 |
| NmuCI | GTSAC | 1 cut(s) | 562 |
| NsiI | ATGCAT | 1 cut(s) | 1615 |
| NspI | RCATGY | 3 cut(s) | 23, 833, 2194 |
| OliI | CACNNNNGTG | 1 cut(s) | 2297 |
| PaeI | GCATGC | 1 cut(s) | 23 |
| PagI | TCATGA | 3 cut(s) | 37, 1303, 2309 |
| PciI | ACATGT | 2 cut(s) | 829, 2190 |
| PctI | GAATGC | 2 cut(s) | 1613, 2112 |
| PfeI | GAWTC | 2 cut(s) | 269, 284 |
| PkrI | GCNGC | 5 cut(s) | 769, 814, 851, 1351, 1917 |
| PleI | GAGTC | 5 cut(s) | 244, 284, 424, 514, 1523 |
| PpsI | GAGTC | 5 cut(s) | 244, 284, 424, 514, 1523 |
| PscI | ACATGT | 2 cut(s) | 829, 2190 |
| PsiI | TTATAA | 1 cut(s) | 1785 |
| Psp6I | CCWGG | 2 cut(s) | 1897, 2241 |
| PspGI | CCWGG | 2 cut(s) | 1897, 2241 |
| PspN4I | GGNNCC | 6 cut(s) | 1140, 1287, 1563, 1650, 1760, 1989 |
| PspPI | GGNCC | 3 cut(s) | 893, 1861, 1987 |
| PstI | CTGCAG | 1 cut(s) | 1465 |
| PsuI | RGATCY | 2 cut(s) | 32, 1285 |
| RsaI | GTAC | 4 cut(s) | 297, 327, 1760, 2147 |
| RsaNI | GTAC | 4 cut(s) | 296, 326, 1759, 2146 |
| RseI | CAYNNNNRTG | 4 cut(s) | 828, 1271, 1745, 2297 |
| SaqAI | TTAA | 4 cut(s) | 189, 503, 1002, 1196 |
| SatI | GCNGC | 5 cut(s) | 768, 813, 850, 1350, 1916 |
| Sau3AI | GATC | 7 cut(s) | 32, 166, 242, 1285, 1933, 1957, 2254 |
| Sau96I | GGNCC | 3 cut(s) | 893, 1861, 1987 |
| SchI | GAGTC | 5 cut(s) | 245, 284, 425, 514, 1523 |
| ScrFI | CCNGG | 2 cut(s) | 1899, 2243 |
| SduI | GDGCHC | 1 cut(s) | 2304 |
| SfaNI | GCATC | 2 cut(s) | 1170, 1600 |
| SfcI | CTRYAG | 1 cut(s) | 1461 |
| SinI | GGWCC | 2 cut(s) | 893, 1987 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 828, 1271, 1745, 2297 |
| SmlI | CTYRAG | 3 cut(s) | 876, 1064, 1355 |
| SmoI | CTYRAG | 3 cut(s) | 876, 1064, 1355 |
| SphI | GCATGC | 1 cut(s) | 23 |
| Sse9I | AATT | 8 cut(s) | 279, 329, 738, 933, 1395, 1541, 1556, 2101 |
| SsiI | CCGC | 7 cut(s) | 429, 544, 754, 767, 1350, 1991, 2142 |
| SspI | AATATT | 1 cut(s) | 645 |
| SspMI | CTAG | 7 cut(s) | 90, 695, 705, 1029, 1316, 1448, 2127 |
| StyD4I | CCNGG | 2 cut(s) | 1897, 2241 |
| StyI | CCWWGG | 3 cut(s) | 841, 1028, 1479 |
| TaaI | ACNGT | 7 cut(s) | 104, 467, 622, 1085, 1270, 1405, 1855 |
| TaiI | ACGT | 2 cut(s) | 598, 1209 |
| TaqI | TCGA | 1 cut(s) | 377 |
| TasI | AATT | 8 cut(s) | 279, 329, 738, 933, 1395, 1541, 1556, 2101 |
| TatI | WGTACW | 2 cut(s) | 295, 325 |
| TauI | GCSGC | 2 cut(s) | 770, 1352 |
| TfiI | GAWTC | 2 cut(s) | 269, 284 |
| Tru1I | TTAA | 4 cut(s) | 189, 503, 1002, 1196 |
| Tru9I | TTAA | 4 cut(s) | 189, 503, 1002, 1196 |
| TscAI | CASTG | 3 cut(s) | 472, 1273, 1410 |
| TseFI | GTSAC | 1 cut(s) | 562 |
| TseI | GCWGC | 3 cut(s) | 812, 849, 1915 |
| Tsp45I | GTSAC | 1 cut(s) | 562 |
| TspDTI | ATGAA | 6 cut(s) | 54, 336, 1292, 1407, 1569, 1900 |
| TspGWI | ACGGA | 3 cut(s) | 266, 885, 899 |
| TspRI | CASTG | 3 cut(s) | 472, 1273, 1410 |
| VneI | GTGCAC | 1 cut(s) | 2300 |
| VpaK11BI | GGWCC | 2 cut(s) | 893, 1987 |
| XapI | RAATTY | 3 cut(s) | 933, 1541, 2101 |
| XceI | RCATGY | 3 cut(s) | 23, 833, 2194 |
| XmaJI | CCTAGG | 1 cut(s) | 1028 |
| XspI | CTAG | 7 cut(s) | 90, 695, 705, 1029, 1316, 1448, 2127 |
| Zsp2I | ATGCAT | 1 cut(s) | 1615 |