Rorug03G0097200

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001825
Physical Location & Seq
Forward (+)
7612320 .. 7613732
1413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0097200.1

Sequence Viewer

Length: 828 bp
ATGGAGTCATTGGAGTATACTTGGCCAGCTTCATGGAATCTTCAGGAAAAGATGAAGGAAGAGCTTGTCCGAGGGCATGAACTTGCAAATCAACTTCACTTGGTTGTCCGTAATGGTGATGGTGAATCGGCCAAAGGTCTTGCTACGAAGATCGTTTGCTCGTTCACTAATAGCCTTTCCCTCTTAGGTGGGAATCATGATTCTAAGGGGAAGCATATTCAAGCCAATACTAGTAGCAATACTGTTGCTCTTCCTACTCTTGTAGATTCATCATCCTGGGTTGCTCTAGAGGCTATTAAGAGAACGGAAATGTTGATAAATAGTAGAAGTTCTTCCAAGAAAAGAAAAACTTCACATTCATGGAGTAGAGACGCTCCCGATTTCATTGATGATGGTCATACATGGAGAAAGTACGGACAGAAACAGATCCTTAATACCAGCCATCCAAGGAGCTACTTTAGGTGCAGTCATAAGTATGATCAAGGCTGCAAGGCGACCAAGCAGGTGCAGAAAGTTGAAGATGATCCATCCTTGTTTCGGACTACATATTTCGGAAATCACACATGCAAACATAGCATACTCAAAGCTCCTGAAAGCATCCTTGATAACGTAAGCGGTGGTAGTCCTAGAGAAGAGTCTAATTCCAAGTTTATGATCACATTTGAGAACAACAACAACTTCCCGTACGAACAAGAACACCCTTTTTTCTCATCCTACTCATCAACCGAAAGAGAGATCATTAAATCTAATCATGTGGCGAGCAGCAGGTCATCATCACTATCTTGTGATTATCTTGTGCCACCTGATCCAGTGATGGTGTTCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

275

Amino Acids

31.08

Weight (kDa)

7.72

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 130 - 190 8.1e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 493
Acc36I ACCTGC 2 cut(s) 493, 756
AccI GTMKAC 1 cut(s) 17
AciI CCGC 1 cut(s) 615
AclWI GGATC 3 cut(s) 421, 518, 800
AcoI YGGCCR 2 cut(s) 23, 129
AcuI CTGAAG 1 cut(s) 26
AfaI GTAC 2 cut(s) 413, 686
AfiI CCNNNNNNNGG 1 cut(s) 537
AgsI TTSAA 2 cut(s) 221, 518
AhlI ACTAGT 1 cut(s) 230
AjnI CCWGG 1 cut(s) 275
AluBI AGCT 4 cut(s) 29, 64, 453, 587
AluI AGCT 4 cut(s) 29, 64, 453, 587
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 3 cut(s) 421, 518, 800
AoxI GGCC 2 cut(s) 23, 129
ApeKI GCWGC 2 cut(s) 486, 762
Asp700I GAANNNNTTC 2 cut(s) 331, 349
AsuHPI GGTGA 2 cut(s) 128, 134
BalI TGGCCA 1 cut(s) 25
BarI GAAGNNNNNNTAC 2 cut(s) 316, 348
BbvI GCAGC 2 cut(s) 473, 774
BccI CCATC 5 cut(s) 113, 386, 450, 535, 808
BciT130I CCWGG 1 cut(s) 277
BclI TGATCA 2 cut(s) 478, 654
BcoDI GTCTC 1 cut(s) 363
BcuI ACTAGT 1 cut(s) 230
BfaI CTAG 3 cut(s) 231, 287, 627
BfuAI ACCTGC 2 cut(s) 493, 756
BisI GCNGC 2 cut(s) 487, 763
BlsI GCNGC 2 cut(s) 488, 764
Bme1390I CCNGG 1 cut(s) 277
BmrFI CCNGG 1 cut(s) 277
BmsI GCATC 1 cut(s) 606
BsaJI CCNNGG 3 cut(s) 70, 276, 446
Bsc4I CCNNNNNNNGG 1 cut(s) 537
Bse1I ACTGG 1 cut(s) 809
BseBI CCWGG 1 cut(s) 277
BseDI CCNNGG 3 cut(s) 70, 276, 446
BseGI GGATG 5 cut(s) 272, 442, 527, 597, 710
BseLI CCNNNNNNNGG 1 cut(s) 537
BseNI ACTGG 1 cut(s) 809
BseXI GCAGC 2 cut(s) 473, 774
BsgI GTGCAG 2 cut(s) 484, 527
BshFI GGCC 2 cut(s) 25, 131
BsiWI CGTACG 1 cut(s) 684
BslI CCNNNNNNNGG 1 cut(s) 537
BsmAI GTCTC 1 cut(s) 363
BsmBI CGTCTC 1 cut(s) 363
BsnI GGCC 2 cut(s) 25, 131
Bsp143I GATC 7 cut(s) 150, 426, 478, 523, 654, 735, 805
BspACI CCGC 1 cut(s) 615
BspANI GGCC 2 cut(s) 25, 131
BspHI TCATGA 1 cut(s) 196
BspMI ACCTGC 2 cut(s) 493, 756
BspPI GGATC 3 cut(s) 421, 518, 800
BspQI GCTCTTC 2 cut(s) 54, 255
BsrI ACTGG 1 cut(s) 809
BssECI CCNNGG 3 cut(s) 70, 276, 446
BssMI GATC 7 cut(s) 150, 426, 478, 523, 654, 735, 805
BssNAI GTATAC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 446
Bst1107I GTATAC 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 277
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 3 cut(s) 54, 255, 627
BstC8I GCNNGC 2 cut(s) 27, 760
BstDEI CTNAG 2 cut(s) 184, 204
BstF5I GGATG 5 cut(s) 272, 442, 527, 597, 710
BstKTI GATC 7 cut(s) 153, 429, 481, 526, 657, 738, 808
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 7 cut(s) 150, 426, 478, 523, 654, 735, 805
BstMWI GCNNNNNNNGC 2 cut(s) 290, 573
BstNI CCWGG 1 cut(s) 277
BstNSI RCATGY 1 cut(s) 567
BstSCI CCNGG 1 cut(s) 275
BstV1I GCAGC 2 cut(s) 473, 774
BstX2I RGATCY 1 cut(s) 426
BstXI CCANNNNNNTGG 1 cut(s) 33
BstYI RGATCY 1 cut(s) 426
BstZ17I GTATAC 1 cut(s) 18
BsuRI GGCC 2 cut(s) 25, 131
BtsCI GGATG 5 cut(s) 272, 442, 527, 597, 710
BtsIMutI CAGTG 1 cut(s) 816
BveI ACCTGC 2 cut(s) 493, 756
Cac8I GCNNGC 2 cut(s) 27, 760
CciI TCATGA 1 cut(s) 196
CseI GACGC 1 cut(s) 380
Csp6I GTAC 2 cut(s) 412, 685
CviAII CATG 7 cut(s) 33, 77, 197, 360, 402, 564, 752
CviQI GTAC 2 cut(s) 412, 685
DdeI CTNAG 2 cut(s) 184, 204
DpnI GATC 7 cut(s) 152, 428, 480, 525, 656, 737, 807
DpnII GATC 7 cut(s) 150, 426, 478, 523, 654, 735, 805
EaeI YGGCCR 2 cut(s) 23, 129
Eam1104I CTCTTC 3 cut(s) 54, 255, 627
EarI CTCTTC 3 cut(s) 54, 255, 627
Eco130I CCWWGG 1 cut(s) 446
Eco57I CTGAAG 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 275
EcoT14I CCWWGG 1 cut(s) 446
ErhI CCWWGG 1 cut(s) 446
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 7 cut(s) 36, 80, 200, 363, 405, 567, 755
FalI AAGNNNNNCTT 2 cut(s) 334, 366
FatI CATG 7 cut(s) 32, 76, 196, 359, 401, 563, 751
FbaI TGATCA 2 cut(s) 478, 654
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 2 cut(s) 487, 763
FokI GGATG 5 cut(s) 259, 429, 514, 584, 697
Fsp4HI GCNGC 2 cut(s) 487, 763
FspBI CTAG 3 cut(s) 231, 287, 627
GluI GCNGC 2 cut(s) 487, 763
HaeIII GGCC 2 cut(s) 25, 131
HgaI GACGC 1 cut(s) 380
Hin1II CATG 7 cut(s) 36, 80, 200, 363, 405, 567, 755
HinfI GANTC 7 cut(s) 5, 37, 125, 193, 200, 266, 635
HphI GGTGA 2 cut(s) 128, 134
Hpy166II GTNNAC 2 cut(s) 18, 165
Hpy188I TCNGA 3 cut(s) 71, 540, 554
Hpy188III TCNNGA 5 cut(s) 44, 197, 287, 377, 590
Hpy8I GTNNAC 2 cut(s) 18, 165
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 5 cut(s) 86, 465, 489, 508, 567
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 573
HpyF3I CTNAG 2 cut(s) 184, 204
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 7 cut(s) 36, 80, 200, 363, 405, 567, 755
Ksp22I TGATCA 2 cut(s) 478, 654
Kzo9I GATC 7 cut(s) 150, 426, 478, 523, 654, 735, 805
LguI GCTCTTC 2 cut(s) 54, 255
LmnI GCTCC 3 cut(s) 379, 450, 592
Lsp1109I GCAGC 2 cut(s) 473, 774
LweI GCATC 1 cut(s) 606
MaeI CTAG 3 cut(s) 231, 287, 627
MaeII ACGT 1 cut(s) 609
MalI GATC 7 cut(s) 152, 428, 480, 525, 656, 737, 807
MboI GATC 7 cut(s) 150, 426, 478, 523, 654, 735, 805
MboII GAAGA 7 cut(s) 32, 71, 160, 242, 324, 530, 644
MflI RGATCY 1 cut(s) 426
MlsI TGGCCA 1 cut(s) 25
MluCI AATT 1 cut(s) 640
MluNI TGGCCA 1 cut(s) 25
MlyI GAGTC 2 cut(s) 14, 644
MnlI CCTC 3 cut(s) 65, 191, 283
Mox20I TGGCCA 1 cut(s) 25
MroXI GAANNNNTTC 2 cut(s) 331, 349
MscI TGGCCA 1 cut(s) 25
MseI TTAA 3 cut(s) 297, 432, 741
MslI CAYNNNNRTG 2 cut(s) 358, 474
Msp20I TGGCCA 1 cut(s) 25
MspR9I CCNGG 1 cut(s) 277
MvaI CCWGG 1 cut(s) 277
MwoI GCNNNNNNNGC 2 cut(s) 290, 573
NdeII GATC 7 cut(s) 150, 426, 478, 523, 654, 735, 805
NlaIII CATG 7 cut(s) 36, 80, 200, 363, 405, 567, 755
NspI RCATGY 1 cut(s) 567
PagI TCATGA 1 cut(s) 196
PaqCI CACCTGC 1 cut(s) 493
PciSI GCTCTTC 2 cut(s) 54, 255
PdmI GAANNNNTTC 2 cut(s) 331, 349
PfeI GAWTC 5 cut(s) 37, 125, 193, 200, 266
Pfl23II CGTACG 1 cut(s) 684
PkrI GCNGC 2 cut(s) 488, 764
PleI GAGTC 2 cut(s) 13, 643
PpsI GAGTC 2 cut(s) 13, 643
Psp6I CCWGG 1 cut(s) 275
PspGI CCWGG 1 cut(s) 275
PspLI CGTACG 1 cut(s) 684
PsuI RGATCY 1 cut(s) 426
RsaI GTAC 2 cut(s) 413, 686
RsaNI GTAC 2 cut(s) 412, 685
RseI CAYNNNNRTG 2 cut(s) 358, 474
SapI GCTCTTC 2 cut(s) 54, 255
SaqAI TTAA 3 cut(s) 297, 432, 741
SatI GCNGC 2 cut(s) 487, 763
Sau3AI GATC 7 cut(s) 150, 426, 478, 523, 654, 735, 805
SchI GAGTC 2 cut(s) 14, 644
ScrFI CCNGG 1 cut(s) 277
SfaNI GCATC 1 cut(s) 606
SmiMI CAYNNNNRTG 2 cut(s) 358, 474
SpeI ACTAGT 1 cut(s) 230
Sse9I AATT 1 cut(s) 640
SsiI CCGC 1 cut(s) 615
SspMI CTAG 3 cut(s) 231, 287, 627
StyD4I CCNGG 1 cut(s) 275
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 1 cut(s) 612
TaqI TCGA 1 cut(s) 822
TasI AATT 1 cut(s) 640
TfiI GAWTC 5 cut(s) 37, 125, 193, 200, 266
Tru1I TTAA 3 cut(s) 297, 432, 741
Tru9I TTAA 3 cut(s) 297, 432, 741
TscAI CASTG 1 cut(s) 816
TseI GCWGC 2 cut(s) 486, 762
TspDTI ATGAA 6 cut(s) 21, 68, 93, 258, 348, 373
TspGWI ACGGA 3 cut(s) 98, 320, 429
TspRI CASTG 1 cut(s) 816
XbaI TCTAGA 1 cut(s) 286
XceI RCATGY 1 cut(s) 567
XmiI GTMKAC 1 cut(s) 17
XmnI GAANNNNTTC 2 cut(s) 331, 349
XspI CTAG 3 cut(s) 231, 287, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.