Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0001825

Physical Location & Seq

Genomic Coordinates Reverse (-)

67582052 .. 67586307

Gene Structure 4256 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG424000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGTCGTACTCGGACTTTGAGCCGGCGTTCTCCTCGGGTCGGGCCAGGCCCCGGCGACCCGACGATCTTCTCAACGGGGACGGTACGGCCAGAGCGGCGTCGTACGGCGGAGGCGGCGTTCACGTGTATTCGACAGTCAATGCGATGAAGCCGGTGGGAGTCCGCGAGCACGTGCGGGAGTTCGCGGCGGCGGCGGCGGAGATGGCGGTGGAGTGCGGCAAGGGGTGTAGGGATATTGTGAGGCAGAGCTTGGGGAATCGGGACTCGTTCGTCTTCCGAAGCTTCGAGAAGGTGAAGGAGTCGTACTGCGGGAAGAGAGTGACGAAGCTGAGCGGGAAGCTGAGCTTTGTGAATGAGTATCTGCCGGAGGATAAGGAGCCGGTTCATGCTTTGTCTGTGATTCTGTTCGTCTCCGTTCTGGCTTTTGCAGCATTGATTCTGAATATGGAAACTACTCCTACCACTCCAGAGGTATTGAAGAAGGTATACATACATCCTCCTAGTGCTGCCCGCGTAATGCTTCCAGATGGTAGATATATTGCATATAAGGAGCAAGGAGTTCCAGTTGACAGAGCTAGATTCTCAATTGTTTCTCCACATTCTTTCCTGTCCTCCAGGCTTGCAGGAATACCGGGACTTAAGGCTTCACTGTTAGAAGAGTTTGGCATTCACTTATTGACATATGATCTTCCTGGATTTGGAGAGAGTGATCCTCATCCCAACAGGAACCTTGAAACTTCAGCAATGGATATGTTGCTCTTAGCTGATGCTGTTGGTGTTAATGACAAGTTTTGGGTTGTTGGATACTCGAGTGGAAGCATGCATGCTTGGGCAGCACTCAGATACATTCCTGATAGACTTGCAGGTGCTGCCATGTTGGCTCCAATGGTTAATCCATACGATTCAATCATGAGCAGGGAAGAGAGACGTAGAACCTGGAAAAGGTGGACAGGCAGAAGGCAACTTTTGTACTTTTTAGCTAGGAGATTTCCCAGATTTCTTTCTTTCTTCTACCAGCAGAGCTTCTTATCTGGAAAGCATGGACAAATCGACAAATGGCTGTCTTTGTCACTGGGAAAGAGGGATAAGGCTTTAATAGAGAGTCCAATATATGAAGAATTCTGGCAAAGGGATGTTGAAGAGTCAATTAGGCAGGGAAGTGCAAAACCTTTTGTGGAGGAAGCTGTTTTACAGGTATCGAATTGGGGTTTCAGCCTTGCAGACCTCAAATTGCAGAAGAAGAAACAGGGAAAAGGAATGCTCCGTTGGCTCAAGTCTGTGCTGAGTGCAGCTCAGGAGGAATATATCGGCTTCCTTGGACCAATACACATATGGCAGGGGATGGATGATAAGGTGGCACCACCATCAATGACTGATTTTGTGCACAGGATTTTACCAGGAGCTGCTGTACATAAGCTCCCCTATGAGGGCCATTTCACCTACATCTACTTTTGTGATGAATGCCATAGACAGATATTCACTACACTTTTTGGAACACCACAAGGGCCAATCAACAATATTGCCACAGAAGTGGATCAAAGTCCTCTCCAAGTTCATACCGAAGAACAGGATGAGATAACACTTGGTGATTCGGTTCTGGCACGAGCTGAGACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6

Protein Analysis

538

Amino Acids

60.41

Weight (kDa)

8.12

Isoelectric Point (pI)

50.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_1	PF00561	213 - 482	1.3e-18	alpha/beta hydrolase fold
Abhydrolase_6	PF12697	215 - 480	2.3e-10	Alpha/beta hydrolase family
Hydrolase_4	PF12146	217 - 313	5e-07	Serine aminopeptidase, S33

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001825)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09690 AT3G09690 AT5G02970
fragaria_vesca	FvH4_3g33440 FvH4_3g33440 FvH4_3g33440 FvH4_3g33440 FvH4_6g15410 FvH4_6g15410 FvH4_6g15410 FvH4_6g15410 FvH4_6g15410
malus_domestica	MD03G1111700.v1.1 MD04G1103800.v1.1 MD11G1126200.v1.1
prunus_persica	Prupe.6G094600_v2.0.a1 Prupe.6G240800_v2.0.a1 Prupe.6G240800_v2.0.a1 Prupe.6G240800_v2.0.a1 Prupe.6G240800_v2.0.a1
pyrus_communis	pycom04g09900 pycom11g10650 pycom12g12240
rosa_chinensis	RchiOBHm_Chr3g0468891 RchiOBHm_Chr5g0060781
rosa_laevigata	RLG00000024403 RLG00000035400
rosa_multiflora	Rmu_co8389401.1_g000001 Rmu_sc0000727.1_g000032 Rmu_sc0001639.1_g000013 Rmu_sc0003298.1_g000004 Rmu_sc0007143.1_g000009
rosa_roxburghii	Rroxscaffold_1G00019940 Rroxscaffold_6G00412480
rosa_rugosa	Rorug03G0097200 Rorug03G0097300 Rorug03G0097400 Rorug05G0334000
rosa_samantha	Rh3AG147000 Rh3BG169500 Rh3DG205600 Rh5AG396700 Rh5BG408700 Rh5CG433400 Rh5DG424000
rosa_wichuraiana	Rw3G013830 Rw5G037400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	854
AasI	GACNNNNNNGTC	1 cut(s)	269
Acc36I	ACCTGC	1 cut(s)	854
AccB1I	GGYRCC	1 cut(s)	1357
AccBSI	CCGCTC	2 cut(s)	95, 333
AccI	GTMKAC	1 cut(s)	486
AccII	CGCG	3 cut(s)	165, 185, 513
AclWI	GGATC	2 cut(s)	704, 1542
AcoI	YGGCCR	1 cut(s)	87
AcsI	RAATTY	1 cut(s)	1118
AcuI	CTGAAG	1 cut(s)	723
AcvI	CACGTG	2 cut(s)	124, 172
AcyI	GRCGYC	1 cut(s)	98
AdeI	CACNNNGTG	1 cut(s)	1586
AfaI	GTAC	6 cut(s)	8, 85, 104, 305, 971, 1410
AfiI	CCNNNNNNNGG	6 cut(s)	39, 51, 698, 942, 1426, 1427
AflII	CTTAAG	1 cut(s)	638
AflIII	ACRYGT	1 cut(s)	123
AgsI	TTSAA	4 cut(s)	478, 734, 906, 1139
AjnI	CCWGG	5 cut(s)	44, 614, 691, 935, 1396
AjuI	GAANNNNNNNTTGG	2 cut(s)	1249, 1281
AleI	CACNNNNGTG	1 cut(s)	1529
AloI	GAACNNNNNNTCC	2 cut(s)	102, 134
Alw21I	GWGCWC	2 cut(s)	171, 1386
Alw26I	GTCTC	3 cut(s)	415, 919, 1604
Alw44I	GTGCAC	1 cut(s)	1382
AlwI	GGATC	2 cut(s)	704, 1542
AlwNI	CAGNNNCTG	2 cut(s)	869, 1403
Ama87I	CYCGRG	2 cut(s)	34, 808
AoxI	GGCC	5 cut(s)	42, 47, 87, 1429, 1505
ApaLI	GTGCAC	1 cut(s)	1382
ApeKI	GCWGC	6 cut(s)	428, 506, 833, 869, 1289, 1403
ApoI	RAATTY	1 cut(s)	1118
AspS9I	GGNCC	5 cut(s)	42, 48, 1319, 1429, 1505
AsuC2I	CCSGG	2 cut(s)	52, 633
AsuHPI	GGTGA	3 cut(s)	304, 1429, 1598
AvaI	CYCGRG	2 cut(s)	34, 808
AvaII	GGWCC	1 cut(s)	1319
BaeGI	GKGCMC	1 cut(s)	1386
BaeI	ACNNNNGTAYC	2 cut(s)	796, 829
BanI	GGYRCC	1 cut(s)	1357
BarI	GAAGNNNNNNTAC	6 cut(s)	287, 319, 470, 502, 1173, 1205
BauI	CACGAG	1 cut(s)	1602
BbrPI	CACGTG	2 cut(s)	124, 172
BbsI	GAAGAC	1 cut(s)	265
Bbv12I	GWGCWC	2 cut(s)	171, 1386
BbvI	GCAGC	6 cut(s)	440, 493, 845, 856, 1301, 1390
BccI	CCATC	4 cut(s)	196, 521, 1336, 1372
BceAI	ACGGC	2 cut(s)	102, 121
BciT130I	CCWGG	5 cut(s)	46, 616, 693, 937, 1398
BciVI	GTATCC	1 cut(s)	797
BcnI	CCSGG	2 cut(s)	52, 633
BcoDI	GTCTC	3 cut(s)	415, 919, 1604
BfaI	CTAG	3 cut(s)	501, 576, 981
BfrI	CTTAAG	1 cut(s)	638
BfuAI	ACCTGC	1 cut(s)	854
BfuI	GTATCC	1 cut(s)	797
BglI	GCCNNNNNGGC	2 cut(s)	95, 878
BlpI	GCTNAGC	2 cut(s)	329, 341
Bme1390I	CCNGG	7 cut(s)	46, 52, 616, 633, 693, 937, 1398
Bme18I	GGWCC	1 cut(s)	1319
BmeT110I	CYCGRG	2 cut(s)	34, 808
BmgT120I	GGNCC	5 cut(s)	42, 48, 1319, 1429, 1505
BmiI	GGNNCC	5 cut(s)	50, 378, 728, 882, 1359
BmrFI	CCNGG	7 cut(s)	46, 52, 616, 633, 693, 937, 1398
BmrI	ACTGGG	1 cut(s)	1082
BmsI	GCATC	1 cut(s)	757
BmuI	ACTGGG	1 cut(s)	1082
BpiI	GAAGAC	1 cut(s)	265
BplI	GAGNNNNNCTC	4 cut(s)	697, 729, 1276, 1308
BpmI	CTGGAG	2 cut(s)	450, 598
Bpu10I	CCTNAGC	1 cut(s)	1293
Bpu1102I	GCTNAGC	2 cut(s)	329, 341
BpuEI	CTTGAG	1 cut(s)	1256
BpuMI	CCSGG	2 cut(s)	52, 633
BsaAI	YACGTR	2 cut(s)	124, 172
BsaBI	GATNNNNATC	1 cut(s)	714
BsaHI	GRCGYC	1 cut(s)	98
BsaJI	CCNNGG	3 cut(s)	33, 50, 1315
BsaXI	ACNNNNNCTCC	8 cut(s)	102, 132, 549, 579, 1392, 1401, 1422, 1431
Bsc4I	CCNNNNNNNGG	6 cut(s)	39, 51, 698, 942, 1426, 1427
Bse118I	RCCGGY	3 cut(s)	22, 151, 379
Bse1I	ACTGG	2 cut(s)	563, 1077
Bse3DI	GCAATG	1 cut(s)	750
Bse8I	GATNNNNATC	1 cut(s)	714
BseBI	CCWGG	5 cut(s)	46, 616, 693, 937, 1398
BseDI	CCNNGG	3 cut(s)	33, 50, 1315
BseGI	GGATG	6 cut(s)	493, 715, 1138, 1347, 1351, 1576
BseJI	GATNNNNATC	1 cut(s)	714
BseLI	CCNNNNNNNGG	6 cut(s)	39, 51, 698, 942, 1426, 1427
BseMI	GCAATG	1 cut(s)	750
BseMII	CTCAG	6 cut(s)	320, 332, 853, 1274, 1307, 1599
BseNI	ACTGG	2 cut(s)	563, 1077
BseRI	GAGGAG	1 cut(s)	22
BseSI	GKGCMC	1 cut(s)	1386
BseXI	GCAGC	6 cut(s)	440, 493, 845, 856, 1301, 1390
BsgI	GTGCAG	1 cut(s)	1308
Bsh1236I	CGCG	3 cut(s)	165, 185, 513
BshFI	GGCC	5 cut(s)	44, 49, 89, 1431, 1507
BshNI	GGYRCC	1 cut(s)	1357
BsiHKAI	GWGCWC	2 cut(s)	171, 1386
BsiHKCI	CYCGRG	2 cut(s)	34, 808
BsiSI	CCGG	6 cut(s)	23, 52, 152, 365, 380, 632
BsiWI	CGTACG	1 cut(s)	102
BslFI	GGGAC	3 cut(s)	92, 275, 648
BslI	CCNNNNNNNGG	6 cut(s)	39, 51, 698, 942, 1426, 1427
BsmAI	GTCTC	3 cut(s)	415, 919, 1604
BsmBI	CGTCTC	2 cut(s)	415, 919
BsmFI	GGGAC	3 cut(s)	92, 275, 648
BsmI	GAATGC	3 cut(s)	666, 1263, 1466
BsnI	GGCC	5 cut(s)	44, 49, 89, 1431, 1507
BsoBI	CYCGRG	2 cut(s)	34, 808
Bsp1286I	GDGCHC	2 cut(s)	171, 1386
Bsp1407I	TGTACA	1 cut(s)	1408
Bsp143I	GATC	4 cut(s)	64, 685, 709, 1534
Bsp1720I	GCTNAGC	2 cut(s)	329, 341
BspANI	GGCC	5 cut(s)	44, 49, 89, 1431, 1507
BspCNI	CTCAG	6 cut(s)	321, 333, 852, 1275, 1306, 1600
BspFNI	CGCG	3 cut(s)	165, 185, 513
BspHI	TCATGA	1 cut(s)	909
BspLI	GGNNCC	5 cut(s)	50, 378, 728, 882, 1359
BspMI	ACCTGC	1 cut(s)	854
BspPI	GGATC	2 cut(s)	704, 1542
BspT107I	GGYRCC	1 cut(s)	1357
BspTI	CTTAAG	1 cut(s)	638
BsrBI	CCGCTC	2 cut(s)	95, 333
BsrDI	GCAATG	1 cut(s)	750
BsrFI	RCCGGY	3 cut(s)	22, 151, 379
BsrGI	TGTACA	1 cut(s)	1408
BsrI	ACTGG	2 cut(s)	563, 1077
BssAI	RCCGGY	3 cut(s)	22, 151, 379
BssECI	CCNNGG	3 cut(s)	33, 50, 1315
BssMI	GATC	4 cut(s)	64, 685, 709, 1534
BssNAI	GTATAC	1 cut(s)	487
BssNI	GRCGYC	1 cut(s)	98
BssSI	CACGAG	1 cut(s)	1602
BssT1I	CCWWGG	1 cut(s)	1315
Bst1107I	GTATAC	1 cut(s)	487
Bst2BI	CACGAG	1 cut(s)	1602
Bst2UI	CCWGG	5 cut(s)	46, 616, 693, 937, 1398
Bst4CI	ACNGT	3 cut(s)	83, 136, 651
Bst6I	CTCTTC	4 cut(s)	308, 651, 915, 1134
BstACI	GRCGYC	1 cut(s)	98
BstAFI	CTTAAG	1 cut(s)	638
BstAPI	GCANNNNNTGC	1 cut(s)	869
BstAUI	TGTACA	1 cut(s)	1408
BstBAI	YACGTR	2 cut(s)	124, 172
BstC8I	GCNNGC	6 cut(s)	24, 167, 511, 621, 821, 825
BstDEI	CTNAG	7 cut(s)	329, 341, 760, 839, 1283, 1293, 1608
BstENI	CCTNNNNNAGG	1 cut(s)	940
BstF5I	GGATG	6 cut(s)	493, 715, 1138, 1347, 1351, 1576
BstFNI	CGCG	3 cut(s)	165, 185, 513
BstKTI	GATC	4 cut(s)	67, 688, 712, 1537
BstMAI	GTCTC	3 cut(s)	415, 919, 1604
BstMBI	GATC	4 cut(s)	64, 685, 709, 1534
BstNI	CCWGG	5 cut(s)	46, 616, 693, 937, 1398
BstNSI	RCATGY	2 cut(s)	823, 827
BstSCI	CCNGG	7 cut(s)	44, 50, 614, 631, 691, 935, 1396
BstSLI	GKGCMC	1 cut(s)	1386
BstUI	CGCG	3 cut(s)	165, 185, 513
BstV1I	GCAGC	6 cut(s)	440, 493, 845, 856, 1301, 1390
BstV2I	GAAGAC	1 cut(s)	265
BstXI	CCANNNNNNTGG	1 cut(s)	1531
BstZ17I	GTATAC	1 cut(s)	487
BsuI	GTATCC	1 cut(s)	797
BsuRI	GGCC	5 cut(s)	44, 49, 89, 1431, 1507
BtgZI	GCGATG	1 cut(s)	158
BtsCI	GGATG	6 cut(s)	493, 715, 1138, 1347, 1351, 1576
BtsIMutI	CAGTG	2 cut(s)	647, 1070
BveI	ACCTGC	1 cut(s)	854
Cac8I	GCNNGC	6 cut(s)	24, 167, 511, 621, 821, 825
CaiI	CAGNNNCTG	2 cut(s)	869, 1403
CciI	TCATGA	1 cut(s)	909
Cfr10I	RCCGGY	3 cut(s)	22, 151, 379
Cfr13I	GGNCC	5 cut(s)	42, 48, 1319, 1429, 1505
CseI	GACGC	1 cut(s)	87
Csp6I	GTAC	6 cut(s)	7, 84, 103, 304, 970, 1409
CviAII	CATG	7 cut(s)	386, 820, 824, 874, 910, 1040, 1614
CviQI	GTAC	6 cut(s)	7, 84, 103, 304, 970, 1409
DdeI	CTNAG	7 cut(s)	329, 341, 760, 839, 1283, 1293, 1608
DpnI	GATC	4 cut(s)	66, 687, 711, 1536
DpnII	GATC	4 cut(s)	64, 685, 709, 1534
DraIII	CACNNNGTG	1 cut(s)	1586
DrdI	GACNNNNNNGTC	1 cut(s)	269
DseDI	GACNNNNNNGTC	1 cut(s)	269
EaeI	YGGCCR	1 cut(s)	87
Eam1104I	CTCTTC	4 cut(s)	308, 651, 915, 1134
EarI	CTCTTC	4 cut(s)	308, 651, 915, 1134
EciI	GGCGGA	2 cut(s)	123, 212
Eco130I	CCWWGG	1 cut(s)	1315
Eco47I	GGWCC	1 cut(s)	1319
Eco57I	CTGAAG	1 cut(s)	723
Eco72I	CACGTG	2 cut(s)	124, 172
Eco88I	CYCGRG	2 cut(s)	34, 808
EcoNI	CCTNNNNNAGG	1 cut(s)	940
EcoO109I	RGGNCCY	1 cut(s)	48
EcoRI	GAATTC	1 cut(s)	1118
EcoRII	CCWGG	5 cut(s)	44, 614, 691, 935, 1396
EcoT14I	CCWWGG	1 cut(s)	1315
EcoT22I	ATGCAT	1 cut(s)	825
ErhI	CCWWGG	1 cut(s)	1315
Esp3I	CGTCTC	2 cut(s)	415, 919
FaeI	CATG	7 cut(s)	389, 823, 827, 877, 913, 1043, 1617
FalI	AAGNNNNNCTT	2 cut(s)	329, 361
FaqI	GGGAC	3 cut(s)	92, 275, 648
FatI	CATG	7 cut(s)	385, 819, 823, 873, 909, 1039, 1613
FauI	CCCGC	4 cut(s)	168, 302, 326, 518
FauNDI	CATATG	2 cut(s)	682, 1331
FblI	GTMKAC	1 cut(s)	486
FokI	GGATG	6 cut(s)	480, 702, 1145, 1354, 1358, 1583
FspBI	CTAG	3 cut(s)	501, 576, 981
GsuI	CTGGAG	2 cut(s)	450, 598
HaeIII	GGCC	5 cut(s)	44, 49, 89, 1431, 1507
HapII	CCGG	6 cut(s)	23, 52, 152, 365, 380, 632
HgaI	GACGC	1 cut(s)	87
Hin1I	GRCGYC	1 cut(s)	98
Hin1II	CATG	7 cut(s)	389, 823, 827, 877, 913, 1043, 1617
HincII	GTYRAC	1 cut(s)	568
HindII	GTYRAC	1 cut(s)	568
HindIII	AAGCTT	1 cut(s)	280
HpaII	CCGG	6 cut(s)	23, 52, 152, 365, 380, 632
HphI	GGTGA	3 cut(s)	304, 1429, 1598
Hpy166II	GTNNAC	5 cut(s)	121, 487, 568, 948, 1384
Hpy188I	TCNGA	4 cut(s)	13, 278, 441, 842
Hpy188III	TCNNGA	8 cut(s)	260, 286, 467, 524, 851, 910, 1032, 1295
Hpy8I	GTNNAC	5 cut(s)	121, 487, 568, 948, 1384
Hpy99I	CGWCG	2 cut(s)	65, 103
HpyAV	CCTTC	4 cut(s)	283, 289, 475, 951
HpyCH4III	ACNGT	3 cut(s)	83, 136, 651
HpyCH4IV	ACGT	3 cut(s)	123, 171, 928
HpyF3I	CTNAG	7 cut(s)	329, 341, 760, 839, 1283, 1293, 1608
HpySE526I	ACGT	3 cut(s)	123, 171, 928
Hsp92I	GRCGYC	1 cut(s)	98
Hsp92II	CATG	7 cut(s)	389, 823, 827, 877, 913, 1043, 1617
KroI	GCCGGC	1 cut(s)	22
KroNI	GCCGGC	1 cut(s)	24
Kzo9I	GATC	4 cut(s)	64, 685, 709, 1534
LmnI	GCTCC	6 cut(s)	376, 550, 886, 1266, 1400, 1422
Lsp1109I	GCAGC	6 cut(s)	440, 493, 845, 856, 1301, 1390
LweI	GCATC	1 cut(s)	757
MaeI	CTAG	3 cut(s)	501, 576, 981
MaeII	ACGT	3 cut(s)	123, 171, 928
MaeIII	GTNAC	2 cut(s)	319, 1068
MalI	GATC	4 cut(s)	66, 687, 711, 1536
MbiI	CCGCTC	2 cut(s)	95, 333
MboI	GATC	4 cut(s)	64, 685, 709, 1534
MfeI	CAATTG	1 cut(s)	585
MhlI	GDGCHC	2 cut(s)	171, 1386
MluCI	AATT	5 cut(s)	585, 1118, 1146, 1201, 1229
MlyI	GAGTC	5 cut(s)	168, 257, 308, 1111, 1151
MmeI	TCCRAC	1 cut(s)	781
Mph1103I	ATGCAT	1 cut(s)	825
MroNI	GCCGGC	1 cut(s)	22
MseI	TTAA	4 cut(s)	639, 780, 891, 1094
MslI	CAYNNNNRTG	1 cut(s)	1529
MspCI	CTTAAG	1 cut(s)	638
MspI	CCGG	6 cut(s)	23, 52, 152, 365, 380, 632
MspR9I	CCNGG	7 cut(s)	46, 52, 616, 633, 693, 937, 1398
MunI	CAATTG	1 cut(s)	585
Mva1269I	GAATGC	3 cut(s)	666, 1263, 1466
MvaI	CCWGG	5 cut(s)	46, 616, 693, 937, 1398
MvnI	CGCG	3 cut(s)	165, 185, 513
NaeI	GCCGGC	1 cut(s)	24
NciI	CCSGG	2 cut(s)	52, 633
NdeI	CATATG	2 cut(s)	682, 1331
NdeII	GATC	4 cut(s)	64, 685, 709, 1534
NgoMIV	GCCGGC	1 cut(s)	22
NlaIII	CATG	7 cut(s)	389, 823, 827, 877, 913, 1043, 1617
NlaIV	GGNNCC	5 cut(s)	50, 378, 728, 882, 1359
NmuCI	GTSAC	2 cut(s)	319, 1068
NsiI	ATGCAT	1 cut(s)	825
NspI	RCATGY	2 cut(s)	823, 827
OliI	CACNNNNGTG	1 cut(s)	1529
PaeI	GCATGC	2 cut(s)	823, 827
PaeR7I	CTCGAG	1 cut(s)	808
PagI	TCATGA	1 cut(s)	909
PaqCI	CACCTGC	1 cut(s)	854
PctI	GAATGC	3 cut(s)	666, 1263, 1466
PdiI	GCCGGC	1 cut(s)	24
PfeI	GAWTC	6 cut(s)	256, 400, 436, 579, 902, 1589
Pfl23II	CGTACG	1 cut(s)	102
PfoI	TCCNGGA	1 cut(s)	691
PleI	GAGTC	5 cut(s)	167, 257, 307, 1110, 1150
PmaCI	CACGTG	2 cut(s)	124, 172
PmlI	CACGTG	2 cut(s)	124, 172
PpsI	GAGTC	5 cut(s)	167, 257, 307, 1110, 1150
Ppu21I	YACGTR	2 cut(s)	124, 172
Psp6I	CCWGG	5 cut(s)	44, 614, 691, 935, 1396
PspCI	CACGTG	2 cut(s)	124, 172
PspGI	CCWGG	5 cut(s)	44, 614, 691, 935, 1396
PspLI	CGTACG	1 cut(s)	102
PspN4I	GGNNCC	5 cut(s)	50, 378, 728, 882, 1359
PspPI	GGNCC	5 cut(s)	42, 48, 1319, 1429, 1505
PspXI	VCTCGAGB	1 cut(s)	808
PstNI	CAGNNNCTG	2 cut(s)	869, 1403
RsaI	GTAC	6 cut(s)	8, 85, 104, 305, 971, 1410
RsaNI	GTAC	6 cut(s)	7, 84, 103, 304, 970, 1409
RseI	CAYNNNNRTG	1 cut(s)	1529
SaqAI	TTAA	4 cut(s)	639, 780, 891, 1094
Sau3AI	GATC	4 cut(s)	64, 685, 709, 1534
Sau96I	GGNCC	5 cut(s)	42, 48, 1319, 1429, 1505
SchI	GAGTC	5 cut(s)	168, 257, 308, 1111, 1151
ScrFI	CCNGG	7 cut(s)	46, 52, 616, 633, 693, 937, 1398
SduI	GDGCHC	2 cut(s)	171, 1386
SfaNI	GCATC	1 cut(s)	757
Sfr274I	CTCGAG	1 cut(s)	808
SinI	GGWCC	1 cut(s)	1319
SlaI	CTCGAG	1 cut(s)	808
SmiMI	CAYNNNNRTG	1 cut(s)	1529
SmlI	CTYRAG	3 cut(s)	638, 808, 1271
SmoI	CTYRAG	3 cut(s)	638, 808, 1271
SphI	GCATGC	2 cut(s)	823, 827
Sse9I	AATT	5 cut(s)	585, 1118, 1146, 1201, 1229
SspI	AATATT	1 cut(s)	1519
SspMI	CTAG	3 cut(s)	501, 576, 981
StyD4I	CCNGG	7 cut(s)	44, 50, 614, 631, 691, 935, 1396
StyI	CCWWGG	1 cut(s)	1315
TaaI	ACNGT	3 cut(s)	83, 136, 651
TaiI	ACGT	3 cut(s)	126, 174, 931
TaqI	TCGA	5 cut(s)	131, 285, 809, 1050, 1199
TasI	AATT	5 cut(s)	585, 1118, 1146, 1201, 1229
TatI	WGTACW	2 cut(s)	969, 1408
TauI	GCSGC	7 cut(s)	98, 117, 188, 191, 194, 197, 219
TfiI	GAWTC	6 cut(s)	256, 400, 436, 579, 902, 1589
Tru1I	TTAA	4 cut(s)	639, 780, 891, 1094
Tru9I	TTAA	4 cut(s)	639, 780, 891, 1094
TscAI	CASTG	2 cut(s)	654, 1077
TseFI	GTSAC	2 cut(s)	319, 1068
TseI	GCWGC	6 cut(s)	428, 506, 833, 869, 1289, 1403
Tsp45I	GTSAC	2 cut(s)	319, 1068
TspDTI	ATGAA	5 cut(s)	161, 374, 1128, 1473, 1544
TspGWI	ACGGA	2 cut(s)	403, 1253
TspRI	CASTG	2 cut(s)	654, 1077
Vha464I	CTTAAG	1 cut(s)	638
VneI	GTGCAC	1 cut(s)	1382
VpaK11BI	GGWCC	1 cut(s)	1319
XagI	CCTNNNNNAGG	1 cut(s)	940
XapI	RAATTY	1 cut(s)	1118
XceI	RCATGY	2 cut(s)	823, 827
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1329
XhoI	CTCGAG	1 cut(s)	808
XmiI	GTMKAC	1 cut(s)	486
XspI	CTAG	3 cut(s)	501, 576, 981
Zsp2I	ATGCAT	1 cut(s)	825

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.