Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003599

Physical Location & Seq

Genomic Coordinates Reverse (-)

20099312 .. 20104193

Gene Structure 4882 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1116200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2760 bp

ATGGATTATCACTTTTTGCCTAAAGATAAGGGAACCAATCATTGGGCATCTGCCAGAGCTCAGGTTGAGAATTTGGCATCTTTTGATGGTGGAACAAGGTACCAGATCCCAGAGGATATGTTTAATAACATTTCTGAGCTTATGAACTTTGATACTTATTCTGGATGGTGCAGCCCAGCAGCAACGGACCAGATTGCAGCCTCTTTAGGGGTGCCATCATGGCCATCAGTGACCTATGCACCTTTTTACGCCTTGAATTTTGTAGAACCGAATGGTGAGCAATTGCCAGTTACGAGTGGTGCTGGAACTTTCAACGTCGGGGAAAATTTCAGTTGTGGAGACAAAATTGTGTTTGATCAAATGGGGACCCCCCAATATGGGGTGTCAACTGATTCCAATGAGGCTAATGACTCAGCTATTAAGTTAAATAATAGTTCTTTTCAACACAATAATCCCATGGACATGGAAAGTTATATGATCTATAGGCCACCTGGGTTGTCACTTAATGAGAAGATGCTTAAGGCACTGTCGATGTTTAAAGAGACTTCCGGTGGCGGTATTTTAGCGCAATTATGGGTTCCCATGAAGCATGGTGATCAGTACTTATTAAGCACTTGTGAGCAACCCTATTTGCTTGACCATATTCTTGCAGGGTATCGTGAAGTCTCAAGGATGTTTACGTTCTCTACAGAAGAAAAACAGAGTTCTATTTTGGGTCTTCCTGGCCGTGTATTTGTCTCCAAAATTCCAGAATGGACTTCAAACGTTATTTTTTACAATAAGACTGAGTACCTACGGGTGGATTATGCAGCTAATCATCAAGTTCGTGGATCAATTGCTTTACCAATTTTTGATTTTGACATGGAAATGTCATGCTGCGCTGTATTGGAACTCGTCAGTACAAAAGAGAAGCCCAATTTTGATACGGAGATGGAAGTAGTTTGCGGTGCGCTCCAGGCTGTTAATTTGAGGACCAATGCACCTCTCCGACTTCTTCCCCAGTGCCTCTCAGAGAACCAAAGAGCTGCTTTAACTGAAATAAGTGATGTCTTACGGGTTGTATGTCATGCGCATTTATTGCCATTGGCTATGACCTGGATTCCTTGTTGCTACTCTGAGGGTAATGGTGATGAAATTAAAAGAGTATGTGCCAAAGGGGGTATTACAAATTCAAATGAGAAATGTATACTATGCATCGAGGAAACAGCTTGTTATGTAAATGATAGCAGAATGCAAGGCTTTGTGCGTGCATGTGCAGAACATCATCTTGAGGAAGGTGAGGGAATTGCTGGGAAAGCACTTCAATCAAATCATCCGTTCTTCATTAATGATGTTAAGGTGTATGATATATGTGAATATCCACTTGTTCATCATGCACGCAAGTATGGTTTGAATGCCGCCGTTGCAATCAGGCTAAGGAGCACCTACACCGCTGATGATGACTATATATTGGAGTTTTTTCTCCCTGTCAATGTGAAGGGGAGTACAGAACAACAACTTCTGTTAAACAACCTCTCAGGTACCATGCAGAAAATTTGTAAAAGTTTAAGGACAGCTTCAGATGCAGAATTAGTTGGGTTAGAAGGCACCAACACTAGGTTTCAGAAGGGGTTGAGCCCCAATGTCCCACAGAGACATTCTCGGACAACACTGTCAGATAGTGAAATGAAATCAGCGAAGAATGTCACCTTTAATGTTTTTAATCGAAAAAATGGGGCAATCAAAAGAGATAATCCTCCTGGAAAGGCACCGAGTGGATCAAGAAGGCAGGTGGAGAAAAAGCGAACTACAACAGAGAAAAATGTGAGCTTGAGTGTTTTACAGCAATACTTTTCTGGTAGTCTCAAGGATGCTGCAAAAAGTATAGGAGTTTGCCCCACTACGCTAAAAAGGATATGCAGACAACATGGTATCTCAAGATGGCCATCTCGTAAAATAAGTAAGGTGAACCGTTCATTGGAGAAAATACAGACTGTGCTTGATTCTGTTCAGGGGGTGGATGGCGGATTGAAGTATGACCCAACTACTGGGGGGTTTGTGGCAACGGGATCCATCATCCAAGAAGTTGATGCGCCCAAAAATATTTTATTTCCTGAGAAGAATCTGTATGTTGAGAACTCTGAACCAGTTACCCAAGATCTTGTTTCAGTATCTTCATTGTCTATCAATAATGGTGGGAGTTTGACTGTTAAGTTGGAAAAGGATGGGAGTTGCGTTCCAACTTCTCATGAGAATGAGGTAAAAAATCAGAGCATCCCTCTCATGCCTTCCAAGCCCATTGCAATAGATTTTGGGTCATGCGATCCTACTAACCGTGGGACGAAGGGCTCTTATCTTGCAAAAGAAGTTAATAAATGGGGTTACACTCAGAACTCTGACTGCCACTTTGTGTCTCAGAGCTCAGGCTTCTTGGTTGCTACTGATGAGATGGATATGGGAGTACATGGAAATGATGGAATTGTTGAATATAACCAGCATAGTTCTTCGAGCTTGACAGATTCATCGAATGACTTTGGTTCAATAATGCAGGGCCACTCATCAAGCTCTCAGAGCTTTGAGGAGCAAAAGCACCCACAAGTCGAGACAACCACTGGTGAGAATGGGTCAAAAATAATTGTGAAAGCTACTTATATAGAGGATACAATCAGGTTCAAGTTTGAGCCTTCTCTTGGGTGCCTTCAACTGTATGAAGAGGTTGCAAAGAGGTTGAAGTTGCAAAGCGGGACATTCCAACTCAGGTATGGATACAAGGAATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PB1 RWP-RK

Protein Analysis

920

Amino Acids

101.79

Weight (kDa)

5.9

Isoelectric Point (pI)

42.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GAF_NLP	PF22922	334 - 517	1.2e-75	NLP protein GAF domain
RWP-RK	PF02042	602 - 648	3.8e-22	RWP-RK domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT3G59580 AT3G59580 AT3G59580
fragaria_vesca	FvH4_1g24430 FvH4_1g24430 FvH4_1g24430
malus_domestica	MD04G1116200.v1.1 MD12G1131800.v1.1
prunus_persica	Prupe.1G200100_v2.0.a1
pyrus_communis	pycom04g10620 pycom12g12770
rosa_chinensis	RchiOBHm_Chr2g0121311
rosa_laevigata	RLG00000018617
rosa_multiflora	Rmu_sc0000079.1_g000028 Rmu_sc0000315.1_g000101
rosa_roxburghii	Rroxscaffold_2G00122460
rosa_rugosa	Rorug02G0233300 Rorug02G0233400 Rorug02G0233500
rosa_samantha	Rh2AG290100 Rh2BG301000 Rh2CG279700 Rh2DG314900
rosa_wichuraiana	Rw2G023260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1759
AasI	GACNNNNNNGTC	1 cut(s)	1651
Acc16I	TGCGCA	1 cut(s)	1071
Acc36I	ACCTGC	1 cut(s)	1759
Acc65I	GGTACC	2 cut(s)	99, 1520
AccB1I	GGYRCC	6 cut(s)	99, 211, 1520, 1586, 1747, 2673
AccB7I	CCANNNNNTGG	2 cut(s)	42, 2027
AccI	GTMKAC	1 cut(s)	1186
AciI	CCGC	6 cut(s)	555, 945, 1398, 1431, 2004, 2721
AclI	AACGTT	1 cut(s)	765
AclWI	GGATC	6 cut(s)	100, 838, 1765, 2043, 2056, 2297
AcoI	YGGCCR	3 cut(s)	221, 724, 1922
AcsI	RAATTY	6 cut(s)	70, 256, 325, 744, 1168, 1533
AcuI	CTGAAG	1 cut(s)	1542
AdeI	CACNNNGTG	2 cut(s)	1754, 2389
AfaI	GTAC	7 cut(s)	101, 602, 791, 901, 1486, 1522, 2442
AflII	CTTAAG	1 cut(s)	518
AjnI	CCWGG	5 cut(s)	490, 721, 954, 1094, 1738
AjuI	GAANNNNNNNTTGG	4 cut(s)	25, 57, 1940, 1972
Alw21I	GWGCWC	3 cut(s)	61, 1424, 2402
Alw26I	GTCTC	8 cut(s)	333, 536, 670, 742, 1627, 1847, 2397, 2576
AlwI	GGATC	6 cut(s)	100, 838, 1765, 2043, 2056, 2297
AoxI	GGCC	5 cut(s)	221, 485, 724, 1922, 2530
ApeKI	GCWGC	7 cut(s)	171, 179, 197, 809, 876, 1025, 1853
ApoI	RAATTY	6 cut(s)	70, 256, 325, 744, 1168, 1533
ArsI	GACNNNNNNTTYG	2 cut(s)	2369, 2401
AseI	ATTAAT	1 cut(s)	1326
Asp718I	GGTACC	2 cut(s)	99, 1520
AspLEI	GCGC	5 cut(s)	568, 881, 952, 1072, 2074
AspS9I	GGNCC	4 cut(s)	187, 366, 972, 2530
AsuHPI	GGTGA	7 cut(s)	287, 605, 1139, 1289, 1678, 1957, 2606
AvaII	GGWCC	3 cut(s)	187, 366, 972
BalI	TGGCCA	2 cut(s)	223, 1924
BamHI	GGATCC	1 cut(s)	2048
BanI	GGYRCC	6 cut(s)	99, 211, 1520, 1586, 1747, 2673
BanII	GRGCYC	4 cut(s)	61, 1619, 2330, 2402
BbsI	GAAGAC	1 cut(s)	710
Bbv12I	GWGCWC	3 cut(s)	61, 1424, 2402
BbvI	GCAGC	7 cut(s)	183, 191, 209, 821, 863, 1012, 1840
BceAI	ACGGC	2 cut(s)	711, 1385
BciT130I	CCWGG	5 cut(s)	492, 723, 956, 1096, 1740
BciVI	GTATCC	2 cut(s)	2632, 2738
BclI	TGATCA	2 cut(s)	355, 595
BcoDI	GTCTC	8 cut(s)	333, 536, 670, 742, 1627, 1847, 2397, 2576
BfaI	CTAG	1 cut(s)	1596
BfmI	CTRYAG	2 cut(s)	481, 687
BfrI	CTTAAG	1 cut(s)	518
BfuAI	ACCTGC	1 cut(s)	1759
BfuI	GTATCC	2 cut(s)	2632, 2738
BglI	GCCNNNNNGGC	1 cut(s)	220
BglII	AGATCT	1 cut(s)	2137
BisI	GCNGC	8 cut(s)	172, 180, 198, 810, 877, 1026, 1398, 1854
BlsI	GCNGC	8 cut(s)	173, 181, 199, 811, 878, 1027, 1399, 1855
BmcAI	AGTACT	1 cut(s)	602
Bme1390I	CCNGG	5 cut(s)	492, 723, 956, 1096, 1740
Bme18I	GGWCC	3 cut(s)	187, 366, 972
BmgT120I	GGNCC	4 cut(s)	187, 366, 972, 2530
BmrFI	CCNGG	5 cut(s)	492, 723, 956, 1096, 1740
BmrI	ACTGGG	2 cut(s)	994, 2037
BmsI	GCATC	8 cut(s)	56, 86, 504, 1203, 1552, 1840, 2059, 2262
BmuI	ACTGGG	2 cut(s)	994, 2037
BpiI	GAAGAC	1 cut(s)	710
BplI	GAGNNNNNCTC	4 cut(s)	1624, 1656, 2242, 2274
BpmI	CTGGAG	1 cut(s)	938
Bpu10I	CCTNAGC	3 cut(s)	60, 1415, 2401
BpuEI	CTTGAG	5 cut(s)	652, 1289, 1829, 1831, 1900
BsaBI	GATNNNNATC	1 cut(s)	1924
BsaJI	CCNNGG	3 cut(s)	456, 491, 2314
BsaWI	WCCGGW	1 cut(s)	548
Bse1I	ACTGG	5 cut(s)	287, 1000, 2032, 2126, 2596
Bse3DI	GCAATG	1 cut(s)	2277
Bse8I	GATNNNNATC	1 cut(s)	1924
BseBI	CCWGG	5 cut(s)	492, 723, 956, 1096, 1740
BseDI	CCNNGG	3 cut(s)	456, 491, 2314
BseGI	GGATG	8 cut(s)	170, 678, 1312, 1855, 2005, 2055, 2209, 2253
BseJI	GATNNNNATC	1 cut(s)	1924
BseMI	GCAATG	1 cut(s)	2277
BseNI	ACTGG	5 cut(s)	287, 1000, 2032, 2126, 2596
BseRI	GAGGAG	1 cut(s)	2573
BseXI	GCAGC	7 cut(s)	183, 191, 209, 821, 863, 1012, 1840
BseYI	CCCAGC	2 cut(s)	175, 1289
BsgI	GTGCAG	2 cut(s)	190, 1275
BshFI	GGCC	5 cut(s)	223, 487, 726, 1924, 2532
BshNI	GGYRCC	6 cut(s)	99, 211, 1520, 1586, 1747, 2673
BsiHKAI	GWGCWC	3 cut(s)	61, 1424, 2402
BsiSI	CCGG	1 cut(s)	549
BslFI	GGGAC	4 cut(s)	379, 1610, 2332, 2737
BsmAI	GTCTC	8 cut(s)	333, 536, 670, 742, 1627, 1847, 2397, 2576
BsmFI	GGGAC	4 cut(s)	379, 1610, 2332, 2737
BsmI	GAATGC	2 cut(s)	1236, 1399
BsnI	GGCC	5 cut(s)	223, 487, 726, 1924, 2532
Bsp1286I	GDGCHC	5 cut(s)	61, 1424, 1619, 2330, 2402
Bsp143I	GATC	9 cut(s)	105, 355, 477, 595, 830, 1757, 2048, 2137, 2302
Bsp19I	CCATGG	1 cut(s)	456
BspACI	CCGC	6 cut(s)	555, 945, 1398, 1431, 2004, 2721
BspANI	GGCC	5 cut(s)	223, 487, 726, 1924, 2532
BspHI	TCATGA	1 cut(s)	2227
BspMI	ACCTGC	1 cut(s)	1759
BspPI	GGATC	6 cut(s)	100, 838, 1765, 2043, 2056, 2297
BspT107I	GGYRCC	6 cut(s)	99, 211, 1520, 1586, 1747, 2673
BspTI	CTTAAG	1 cut(s)	518
BsrDI	GCAATG	1 cut(s)	2277
BsrI	ACTGG	5 cut(s)	287, 1000, 2032, 2126, 2596
BssECI	CCNNGG	3 cut(s)	456, 491, 2314
BssMI	GATC	9 cut(s)	105, 355, 477, 595, 830, 1757, 2048, 2137, 2302
BssNAI	GTATAC	1 cut(s)	1187
BssT1I	CCWWGG	1 cut(s)	456
Bst1107I	GTATAC	1 cut(s)	1187
Bst2UI	CCWGG	5 cut(s)	492, 723, 956, 1096, 1740
Bst4CI	ACNGT	7 cut(s)	528, 1653, 1952, 1975, 2188, 2315, 2685
Bst6I	CTCTTC	1 cut(s)	2685
BstAFI	CTTAAG	1 cut(s)	518
BstAPI	GCANNNNNTGC	1 cut(s)	1078
BstC8I	GCNNGC	2 cut(s)	1248, 1378
BstDSI	CCRYGG	2 cut(s)	456, 2314
BstF5I	GGATG	8 cut(s)	170, 678, 1312, 1855, 2005, 2055, 2209, 2253
BstHHI	GCGC	5 cut(s)	568, 881, 952, 1072, 2074
BstKTI	GATC	9 cut(s)	108, 358, 480, 598, 833, 1760, 2051, 2140, 2305
BstMAI	GTCTC	8 cut(s)	333, 536, 670, 742, 1627, 1847, 2397, 2576
BstMBI	GATC	9 cut(s)	105, 355, 477, 595, 830, 1757, 2048, 2137, 2302
BstMWI	GCNNNNNNNGC	8 cut(s)	220, 956, 1078, 1295, 1403, 1562, 2272, 2550
BstNI	CCWGG	5 cut(s)	492, 723, 956, 1096, 1740
BstNSI	RCATGY	1 cut(s)	1254
BstSCI	CCNGG	5 cut(s)	490, 721, 954, 1094, 1738
BstSFI	CTRYAG	2 cut(s)	481, 687
BstV1I	GCAGC	7 cut(s)	183, 191, 209, 821, 863, 1012, 1840
BstV2I	GAAGAC	1 cut(s)	710
BstX2I	RGATCY	3 cut(s)	105, 2048, 2137
BstXI	CCANNNNNNTGG	1 cut(s)	463
BstYI	RGATCY	3 cut(s)	105, 2048, 2137
BstZ17I	GTATAC	1 cut(s)	1187
BsuI	GTATCC	2 cut(s)	2632, 2738
BsuRI	GGCC	5 cut(s)	223, 487, 726, 1924, 2532
BtgI	CCRYGG	2 cut(s)	456, 2314
BtsCI	GGATG	8 cut(s)	170, 678, 1312, 1855, 2005, 2055, 2209, 2253
BtsIMutI	CAGTG	5 cut(s)	234, 524, 1007, 1649, 2589
BveI	ACCTGC	1 cut(s)	1759
Cac8I	GCNNGC	2 cut(s)	1248, 1378
CciI	TCATGA	1 cut(s)	2227
CfoI	GCGC	5 cut(s)	568, 881, 952, 1072, 2074
Cfr13I	GGNCC	4 cut(s)	187, 366, 972, 2530
Csp6I	GTAC	7 cut(s)	100, 601, 790, 900, 1485, 1521, 2441
CspCI	CAANNNNNGTGG	4 cut(s)	477, 512, 2155, 2190
CviQI	GTAC	7 cut(s)	100, 601, 790, 900, 1485, 1521, 2441
DpnI	GATC	9 cut(s)	107, 357, 479, 597, 832, 1759, 2050, 2139, 2304
DpnII	GATC	9 cut(s)	105, 355, 477, 595, 830, 1757, 2048, 2137, 2302
DraI	TTTAAA	1 cut(s)	538
DraIII	CACNNNGTG	2 cut(s)	1754, 2389
DrdI	GACNNNNNNGTC	1 cut(s)	1651
DseDI	GACNNNNNNGTC	1 cut(s)	1651
EaeI	YGGCCR	3 cut(s)	221, 724, 1922
Eam1104I	CTCTTC	1 cut(s)	2685
EarI	CTCTTC	1 cut(s)	2685
EciI	GGCGGA	1 cut(s)	2019
Ecl136II	GAGCTC	2 cut(s)	59, 2400
Eco130I	CCWWGG	1 cut(s)	456
Eco24I	GRGCYC	4 cut(s)	61, 1619, 2330, 2402
Eco47I	GGWCC	3 cut(s)	187, 366, 972
Eco53kI	GAGCTC	2 cut(s)	59, 2400
Eco57I	CTGAAG	1 cut(s)	1542
EcoICRI	GAGCTC	2 cut(s)	59, 2400
EcoO109I	RGGNCCY	1 cut(s)	366
EcoRII	CCWGG	5 cut(s)	490, 721, 954, 1094, 1738
EcoT14I	CCWWGG	1 cut(s)	456
EcoT22I	ATGCAT	1 cut(s)	1196
EcoT38I	GRGCYC	4 cut(s)	61, 1619, 2330, 2402
ErhI	CCWWGG	1 cut(s)	456
FalI	AAGNNNNNCTT	4 cut(s)	1012, 1044, 1540, 1572
FaqI	GGGAC	4 cut(s)	379, 1610, 2332, 2737
FauI	CCCGC	1 cut(s)	2714
FbaI	TGATCA	2 cut(s)	355, 595
FblI	GTMKAC	1 cut(s)	1186
Fnu4HI	GCNGC	8 cut(s)	172, 180, 198, 810, 877, 1026, 1398, 1854
FokI	GGATG	8 cut(s)	177, 685, 1299, 1862, 2012, 2042, 2216, 2240
FriOI	GRGCYC	4 cut(s)	61, 1619, 2330, 2402
Fsp4HI	GCNGC	8 cut(s)	172, 180, 198, 810, 877, 1026, 1398, 1854
FspAI	RTGCGCAY	1 cut(s)	1071
FspBI	CTAG	1 cut(s)	1596
FspI	TGCGCA	1 cut(s)	1071
GlaI	GCGC	5 cut(s)	567, 880, 951, 1071, 2073
GluI	GCNGC	8 cut(s)	172, 180, 198, 810, 877, 1026, 1398, 1854
GsaI	CCCAGC	2 cut(s)	179, 1293
GsuI	CTGGAG	1 cut(s)	938
HaeIII	GGCC	5 cut(s)	223, 487, 726, 1924, 2532
HapII	CCGG	1 cut(s)	549
HhaI	GCGC	5 cut(s)	568, 881, 952, 1072, 2074
Hin6I	GCGC	5 cut(s)	566, 879, 950, 1070, 2072
HinP1I	GCGC	5 cut(s)	566, 879, 950, 1070, 2072
HincII	GTYRAC	1 cut(s)	387
HindII	GTYRAC	1 cut(s)	387
HinfI	GANTC	6 cut(s)	392, 410, 1099, 1982, 2101, 2498
HpaII	CCGG	1 cut(s)	549
HphI	GGTGA	7 cut(s)	287, 605, 1139, 1289, 1678, 1957, 2606
Hpy166II	GTNNAC	4 cut(s)	387, 678, 1187, 1948
Hpy188III	TCNNGA	9 cut(s)	162, 659, 749, 1268, 1761, 1917, 2093, 2228, 2581
Hpy8I	GTNNAC	4 cut(s)	387, 678, 1187, 1948
Hpy99I	CGWCG	1 cut(s)	320
HpyAV	CCTTC	9 cut(s)	1268, 1471, 1577, 1600, 1758, 2277, 2317, 2673, 2687
HpyCH4III	ACNGT	7 cut(s)	528, 1653, 1952, 1975, 2188, 2315, 2685
HpyCH4IV	ACGT	3 cut(s)	315, 680, 765
HpyF10VI	GCNNNNNNNGC	8 cut(s)	220, 956, 1078, 1295, 1403, 1562, 2272, 2550
HpySE526I	ACGT	3 cut(s)	315, 680, 765
HspAI	GCGC	5 cut(s)	566, 879, 950, 1070, 2072
KflI	GGGWCCC	1 cut(s)	366
KpnI	GGTACC	2 cut(s)	103, 1524
Ksp22I	TGATCA	2 cut(s)	355, 595
Kzo9I	GATC	9 cut(s)	105, 355, 477, 595, 830, 1757, 2048, 2137, 2302
LmnI	GCTCC	3 cut(s)	957, 1419, 2560
Lsp1109I	GCAGC	7 cut(s)	183, 191, 209, 821, 863, 1012, 1840
LweI	GCATC	8 cut(s)	56, 86, 504, 1203, 1552, 1840, 2059, 2262
MaeI	CTAG	1 cut(s)	1596
MaeII	ACGT	3 cut(s)	315, 680, 765
MaeIII	GTNAC	6 cut(s)	229, 289, 498, 1684, 2128, 2360
MalI	GATC	9 cut(s)	107, 357, 479, 597, 832, 1759, 2050, 2139, 2304
MboI	GATC	9 cut(s)	105, 355, 477, 595, 830, 1757, 2048, 2137, 2302
MfeI	CAATTG	2 cut(s)	281, 834
MflI	RGATCY	3 cut(s)	105, 2048, 2137
MhlI	GDGCHC	5 cut(s)	61, 1424, 1619, 2330, 2402
MlsI	TGGCCA	2 cut(s)	223, 1924
MluNI	TGGCCA	2 cut(s)	223, 1924
MlyI	GAGTC	1 cut(s)	404
MmeI	TCCRAC	4 cut(s)	1012, 2175, 2243, 2755
Mox20I	TGGCCA	2 cut(s)	223, 1924
Mph1103I	ATGCAT	1 cut(s)	1196
MscI	TGGCCA	2 cut(s)	223, 1924
MslI	CAYNNNNRTG	4 cut(s)	461, 866, 2232, 2448
Msp20I	TGGCCA	2 cut(s)	223, 1924
MspA1I	CMGCKG	1 cut(s)	1433
MspCI	CTTAAG	1 cut(s)	518
MspI	CCGG	1 cut(s)	549
MspR9I	CCNGG	5 cut(s)	492, 723, 956, 1096, 1740
MunI	CAATTG	2 cut(s)	281, 834
Mva1269I	GAATGC	2 cut(s)	1236, 1399
MvaI	CCWGG	5 cut(s)	492, 723, 956, 1096, 1740
MwoI	GCNNNNNNNGC	8 cut(s)	220, 956, 1078, 1295, 1403, 1562, 2272, 2550
NcoI	CCATGG	1 cut(s)	456
NdeII	GATC	9 cut(s)	105, 355, 477, 595, 830, 1757, 2048, 2137, 2302
NmuCI	GTSAC	3 cut(s)	229, 498, 1684
NsbI	TGCGCA	1 cut(s)	1071
NsiI	ATGCAT	1 cut(s)	1196
NspI	RCATGY	1 cut(s)	1254
PagI	TCATGA	1 cut(s)	2227
PaqCI	CACCTGC	1 cut(s)	1759
PctI	GAATGC	2 cut(s)	1236, 1399
PfeI	GAWTC	5 cut(s)	392, 1099, 1982, 2101, 2498
PflMI	CCANNNNNTGG	2 cut(s)	42, 2027
PfoI	TCCNGGA	1 cut(s)	1738
PkrI	GCNGC	8 cut(s)	173, 181, 199, 811, 878, 1027, 1399, 1855
PleI	GAGTC	1 cut(s)	404
PpsI	GAGTC	1 cut(s)	404
PpuMI	RGGWCCY	1 cut(s)	366
PshBI	ATTAAT	1 cut(s)	1326
Psp124BI	GAGCTC	2 cut(s)	61, 2402
Psp1406I	AACGTT	1 cut(s)	765
Psp5II	RGGWCCY	1 cut(s)	366
Psp6I	CCWGG	5 cut(s)	490, 721, 954, 1094, 1738
PspFI	CCCAGC	2 cut(s)	175, 1289
PspGI	CCWGG	5 cut(s)	490, 721, 954, 1094, 1738
PspPI	GGNCC	4 cut(s)	187, 366, 972, 2530
PspPPI	RGGWCCY	1 cut(s)	366
PsuI	RGATCY	3 cut(s)	105, 2048, 2137
RsaI	GTAC	7 cut(s)	101, 602, 791, 901, 1486, 1522, 2442
RsaNI	GTAC	7 cut(s)	100, 601, 790, 900, 1485, 1521, 2441
RseI	CAYNNNNRTG	4 cut(s)	461, 866, 2232, 2448
SacI	GAGCTC	2 cut(s)	61, 2402
SatI	GCNGC	8 cut(s)	172, 180, 198, 810, 877, 1026, 1398, 1854
Sau3AI	GATC	9 cut(s)	105, 355, 477, 595, 830, 1757, 2048, 2137, 2302
Sau96I	GGNCC	4 cut(s)	187, 366, 972, 2530
ScaI	AGTACT	1 cut(s)	602
SchI	GAGTC	1 cut(s)	404
ScrFI	CCNGG	5 cut(s)	492, 723, 956, 1096, 1740
SduI	GDGCHC	5 cut(s)	61, 1424, 1619, 2330, 2402
SfaNI	GCATC	8 cut(s)	56, 86, 504, 1203, 1552, 1840, 2059, 2262
SfcI	CTRYAG	2 cut(s)	481, 687
SinI	GGWCC	3 cut(s)	187, 366, 972
SmiMI	CAYNNNNRTG	4 cut(s)	461, 866, 2232, 2448
SmlI	CTYRAG	6 cut(s)	518, 667, 1268, 1810, 1844, 1915
SmoI	CTYRAG	6 cut(s)	518, 667, 1268, 1810, 1844, 1915
SsiI	CCGC	6 cut(s)	555, 945, 1398, 1431, 2004, 2721
SspI	AATATT	1 cut(s)	2083
SspMI	CTAG	1 cut(s)	1596
SstI	GAGCTC	2 cut(s)	61, 2402
StyD4I	CCNGG	5 cut(s)	490, 721, 954, 1094, 1738
StyI	CCWWGG	1 cut(s)	456
TaaI	ACNGT	7 cut(s)	528, 1653, 1952, 1975, 2188, 2315, 2685
TaiI	ACGT	3 cut(s)	318, 683, 768
TaqI	TCGA	6 cut(s)	530, 1197, 1705, 2486, 2504, 2580
TatI	WGTACW	4 cut(s)	600, 899, 1484, 2440
TauI	GCSGC	1 cut(s)	1400
TfiI	GAWTC	5 cut(s)	392, 1099, 1982, 2101, 2498
TscAI	CASTG	5 cut(s)	234, 531, 1007, 1656, 2596
TseFI	GTSAC	3 cut(s)	229, 498, 1684
TseI	GCWGC	7 cut(s)	171, 179, 197, 809, 876, 1025, 1853
Tsp45I	GTSAC	3 cut(s)	229, 498, 1684
TspGWI	ACGGA	3 cut(s)	200, 941, 1305
TspRI	CASTG	5 cut(s)	234, 531, 1007, 1656, 2596
Van91I	CCANNNNNTGG	2 cut(s)	42, 2027
Vha464I	CTTAAG	1 cut(s)	518
VpaK11BI	GGWCC	3 cut(s)	187, 366, 972
VspI	ATTAAT	1 cut(s)	1326
XapI	RAATTY	6 cut(s)	70, 256, 325, 744, 1168, 1533
XceI	RCATGY	1 cut(s)	1254
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2738
XmiI	GTMKAC	1 cut(s)	1186
XspI	CTAG	1 cut(s)	1596
ZrmI	AGTACT	1 cut(s)	602
Zsp2I	ATGCAT	1 cut(s)	1196

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.