Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0003599

Physical Location & Seq

Genomic Coordinates Reverse (-)

32768946 .. 32771855

Gene Structure 2910 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018617

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2910 bp

ATGGAATACCAGTTTTCGTCTGGAGAGGGGAAGGATCAGGGGTCGTCTTCTGGAGCTGAGGTGGAGGGTTTGGTGCCACTTGATGGTGCAGTGAGGAACTCGATTTCGGAGGACATGTTTAACAACATTGCTGAGTTTATGAATTTTGATTCTTATGCTGGATGGTTCAGCAGCCCGGGAACAATGGAGCAGATTGCGGCGGCGTATCCATCGGTGAGTTATGCTCCTTTGGATGCCTTCAACTTCACACAACAGAACGGTGGAACATTGCCTGTGACTGAAGATGGTGCGACTTTTGATAAGGCTGGGAGTTCTTTTGATTGTGGTAACAAGATTGTGTTTCAGCAAACCCAATACGGGGGGGCGTCAACTGATTCCCACCACACTCATGATGCAGCTGCTAAACCGAAGAATGGTTCTTTTCAACAGAATAATGTCATGGATATGGCAGACTGTGTGATCCCCAGGCCATATGGATGGTCACTTAATGAGAAGATGCTAAAGGCGTTGTCCTTTTTCAAGGAGTCTTTTGGTGGGGGAATTTTGGCACAAGTATGGGTTCCCATGAAGCATGGTGATCACTCCATCTTAAGCACTTGCGAACAGCCCTATTTGCTTGACCATGTTCTTGCAGGGTATCGGGAAGTGTCGAGGATGTTTACATTTGCTGCAGAAGAAAGGCAGGGTACTAGCCTGGGGCTTCCTGGCCGTGTATTTGTCTCCAAAGTTCCAGAGTGGACATCAAATGTTAGCTGTTACAACAAGGACGAGTACCTGAGGGTGGAACATGCAGCTAATCATCAGGTCCGTGGATCAATTGCTTTGCCGGTTTTTGATCTGAACTCTGAGATGTCATGTTGTGGTGTGTTTGAACTTGTTAGTACAAAAGATAAACTCAACTTTGATGCAGAGATGGAGATGGTTTGTGAGGCCCTTCAGGCTGTGAAGTTGAGGACCACTACGCCTCCCCGACTTCATCCCCAGTGCCTCTCAAAGAACCAAAGGGCTGCTTTAACTGAAATTACTGATGTCTTACGAGCTGTGTGTCATGCACATACATTGCCGTTGGCTCTCACCTGGATTCCTTGTTGCTACTCTGAGGGTGATGCAGAGGAAATTAGAAGAGTACGTGTTCGAGGGGGTATTACAAAATCAAATGAAAAATGCATACTATGCATTGAGGAAACAGCTTGTTATATAAATGATAGAGCGATGCAAGGCTTTGTGCATGCATGTGCAGAACATCATCTTGAAGAAGGAAATGGTATTGCTGGGAAAGCACTTCAATCAAATCAACCCTTCTTCATTCATGATGTGAAGGTGTATGACATATATGACTATCCACTAGTTCATCATGCACGCAGGTATGGTTTGAACGCTGCTGTTGCAATCAGACTAAGGAGCACCTACACTGGTGATGATGACTATATCTTAGAGTTTTTTCTGCCTGTCAATATGAAGGGCAGTTCTGAACAGCAACTTTTGTTAAACAACCTCTCGGGTACCATGCAGAGAATATGTAAGAGTTTAAGGACAGTGTCAGATGCAGAATTCGCTGGGGTTGAAGGCTCCGACACTGGGTTTCAAAGGGAGGCAGTCTCGAATGCCCCACCAATCCCCAGGAGAGATTCTCAGACAACGTCATCGGATAGTGAAATGAAGTTAACTGAGAAGATGGCCTCAAATGTTTATAATAGAAGAGATGGTGGAGTCGAACTAGATTTTCCTTCTGAACAGGCACCACTTGGATCAAGAAGGCAGGTGGAGAAAAAGCGTAGTACAGCAGAGAAAAATGTGAGCTTGAGTGTTCTACAGCAATACTTCTCTGGGAGTCTCAAGGATGCTGCAAAAAGCATTGGAGTCTGCCCCACTACACTAAAAAGGATCTGCAGACAACACGGGATCCCAAGATGGCCATCTCGTAAAATAAATAAGGTGAATCGTTCCTTGAAGAAAATACAAACTGTACTTGATTCTGTTCAGGGGGTGGAAGGAGGATTGAAGTATGACCCAACCACTGGAGGGTTTGTGGCAGCAGGGTCTACCATCCAAGAGTTTGATGCACAGAAAAATCTTTTCTTCTCTGAGAAGAACTTGCCTGCTCAGAATGTTGTTCCAGTTCCCGAATATCCAGTTTCAGCACCTTCAAAGTTTTGTAATGATGGTGAGAGTTTGGATATTATGTTGGAAGAGGATGGCTGTTGTATGAATGAACGTGTGTTGGTTCCAACTGCTCATCAGGCCAAGGAAGAGGTAAGTGAACAACACATCCCTGTGGTTGATTGTAGTATGGATTCCAAGCCGATTGCAATTGATTTTGGATCATGCCAGCCGACTGACCATGACATCCTGCCTCATACTTGTCCGGAAAGTGCTTTTGGGGTCTCATATTTGGTAAATGAAGTCAACAAATGGGATCAAAGCAAGGGGAGTCCAAAATTAGAGAGTTCTGACTGCCATGTTGTGTCTCAGAGCTCTTCTTCAAGCATGACGCACTCATCAAATAGTTCTGGTTCAATGATGCATTGTTGTTCGTCGAGTTCTCGGAGCTTTGAGGAGCGAAAGTACCCTAAGGTCAAAGCAAGAAATGATGAGAATGGATCGAAAATCATTGTGAAAGCTACTTATAAAGAAGATACAATTAGGTTCAAGTTTGAGCCTTCGAGTGGATGTCTTAAACTGTATGAAGAAGTTGCAAAGAGGTTGAGGTTGCAAGATGGAACTTTCCAGCTCAAGTATCTAGATGATGAACAAGAATGGGTGATGTTAGTCAGTGATGCAGATTTGCAGGAGTGCCTTGAGATATTGGATGACATTGGGACACGTAGTGTTAAGTTTATGGTTCGAGATATACCTTTTGATCTAAGCAGTTCCGGCAGTAGTAATTGTTTTCTGGCCGGGGGATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PB1 RWP-RK

Protein Analysis

970

Amino Acids

107.11

Weight (kDa)

5.44

Isoelectric Point (pI)

50.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GAF_NLP	PF22922	328 - 511	2.3e-76	NLP protein GAF domain
RWP-RK	PF02042	599 - 645	2.4e-22	RWP-RK domain
PB1	PF00564	871 - 949	9.7e-19	PB1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT3G59580 AT3G59580 AT3G59580
fragaria_vesca	FvH4_1g24430 FvH4_1g24430 FvH4_1g24430
malus_domestica	MD04G1116200.v1.1 MD12G1131800.v1.1
prunus_persica	Prupe.1G200100_v2.0.a1
pyrus_communis	pycom04g10620 pycom12g12770
rosa_chinensis	RchiOBHm_Chr2g0121311
rosa_laevigata	RLG00000018617
rosa_multiflora	Rmu_sc0000079.1_g000028 Rmu_sc0000315.1_g000101
rosa_roxburghii	Rroxscaffold_2G00122460
rosa_rugosa	Rorug02G0233300 Rorug02G0233400 Rorug02G0233500
rosa_samantha	Rh2AG290100 Rh2BG301000 Rh2CG279700 Rh2DG314900
rosa_wichuraiana	Rw2G023260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1692, 2628
AarI	CACCTGC	1 cut(s)	1750
Acc36I	ACCTGC	2 cut(s)	1353, 1750
Acc65I	GGTACC	1 cut(s)	1502
AccB1I	GGYRCC	3 cut(s)	73, 1502, 1738
AccB7I	CCANNNNNTGG	2 cut(s)	83, 2018
AccI	GTMKAC	1 cut(s)	2042
AccIII	TCCGGA	1 cut(s)	2365
AciI	CCGC	2 cut(s)	197, 200
AcoI	YGGCCR	3 cut(s)	706, 1913, 2895
AcsI	RAATTY	3 cut(s)	142, 540, 1550
AcuI	CTGAAG	2 cut(s)	300, 920
AcyI	GRCGYC	1 cut(s)	365
AdeI	CACNNNGTG	1 cut(s)	2828
AfaI	GTAC	8 cut(s)	688, 773, 883, 1128, 1504, 1780, 1968, 2567
AfiI	CCNNNNNNNGG	9 cut(s)	83, 357, 358, 413, 781, 1578, 2018, 2022, 2666
AflII	CTTAAG	1 cut(s)	589
AflIII	ACRYGT	4 cut(s)	114, 1129, 2215, 2822
AhlI	ACTAGT	1 cut(s)	1345
AjnI	CCWGG	5 cut(s)	464, 693, 703, 1076, 1619
AjuI	GAANNNNNNNTTGG	2 cut(s)	2361, 2393
AloI	GAACNNNNNNTCC	2 cut(s)	2253, 2285
Alw21I	GWGCWC	2 cut(s)	1406, 2477
Alw26I	GTCTC	5 cut(s)	724, 1603, 1838, 2389, 2472
Ama87I	CYCGRG	2 cut(s)	175, 1498
Aor13HI	TCCGGA	1 cut(s)	2365
AoxI	GGCC	7 cut(s)	467, 706, 930, 1677, 1913, 2241, 2895
ApeKI	GCWGC	9 cut(s)	171, 395, 398, 668, 791, 1007, 1379, 1844, 2033
ApoI	RAATTY	3 cut(s)	142, 540, 1550
Asp718I	GGTACC	1 cut(s)	1502
AspS9I	GGNCC	3 cut(s)	805, 931, 954
AsuC2I	CCSGG	3 cut(s)	176, 177, 2899
AsuHPI	GGTGA	8 cut(s)	226, 587, 1066, 1115, 1427, 1948, 2177, 2773
AvaI	CYCGRG	2 cut(s)	175, 1498
AvaII	GGWCC	2 cut(s)	805, 954
AxyI	CCTNAGG	2 cut(s)	776, 2571
BalI	TGGCCA	1 cut(s)	1915
BamHI	GGATCC	1 cut(s)	1902
BanI	GGYRCC	3 cut(s)	73, 1502, 1738
BanII	GRGCYC	1 cut(s)	2477
BbsI	GAAGAC	1 cut(s)	39
Bbv12I	GWGCWC	2 cut(s)	1406, 2477
BbvCI	CCTCAGC	1 cut(s)	57
BbvI	GCAGC	9 cut(s)	183, 385, 407, 655, 803, 994, 1366, 1831, 2045
BceAI	ACGGC	2 cut(s)	693, 1048
BciT130I	CCWGG	5 cut(s)	466, 695, 705, 1078, 1621
BciVI	GTATCC	1 cut(s)	216
BclI	TGATCA	1 cut(s)	577
BcnI	CCSGG	3 cut(s)	176, 177, 2899
BcoDI	GTCTC	5 cut(s)	724, 1603, 1838, 2389, 2472
BcuI	ACTAGT	1 cut(s)	1345
BfaI	CTAG	4 cut(s)	690, 1346, 1718, 2741
BfmI	CTRYAG	3 cut(s)	669, 1811, 1888
BfrI	CTTAAG	1 cut(s)	589
BfuAI	ACCTGC	2 cut(s)	1353, 1750
BfuI	GTATCC	1 cut(s)	216
BglI	GCCNNNNNGGC	1 cut(s)	938
Bme1390I	CCNGG	8 cut(s)	176, 177, 466, 695, 705, 1078, 1621, 2899
Bme18I	GGWCC	2 cut(s)	805, 954
BmeT110I	CYCGRG	2 cut(s)	175, 1498
BmgT120I	GGNCC	3 cut(s)	805, 931, 954
BmiI	GGNNCC	7 cut(s)	75, 561, 1504, 1570, 1740, 1904, 2226
BmrFI	CCNGG	8 cut(s)	176, 177, 466, 695, 705, 1078, 1621, 2899
BmrI	ACTGGG	2 cut(s)	976, 1587
BmuI	ACTGGG	2 cut(s)	976, 1587
BpiI	GAAGAC	1 cut(s)	39
BplI	GAGNNNNNCTC	6 cut(s)	208, 240, 1583, 1615, 1615, 1647
BpmI	CTGGAG	3 cut(s)	42, 72, 2040
Bpu10I	CCTNAGC	1 cut(s)	57
BpuEI	CTTGAG	4 cut(s)	1820, 1822, 2717, 2819
BpuMI	CCSGG	3 cut(s)	176, 177, 2899
BsaAI	YACGTR	2 cut(s)	1130, 2825
BsaBI	GATNNNNATC	1 cut(s)	1915
BsaHI	GRCGYC	1 cut(s)	365
BsaI	GGTCTC	1 cut(s)	2389
BsaJI	CCNNGG	7 cut(s)	175, 464, 694, 808, 1619, 2244, 2898
BsaWI	WCCGGW	1 cut(s)	2365
Bsc4I	CCNNNNNNNGG	9 cut(s)	83, 357, 358, 413, 781, 1578, 2018, 2022, 2666
Bse118I	RCCGGY	1 cut(s)	826
Bse1I	ACTGG	7 cut(s)	10, 982, 1417, 1582, 2023, 2117, 2132
Bse21I	CCTNAGG	2 cut(s)	776, 2571
Bse3DI	GCAATG	3 cut(s)	126, 266, 1058
Bse8I	GATNNNNATC	1 cut(s)	1915
BseAI	TCCGGA	1 cut(s)	2365
BseBI	CCWGG	5 cut(s)	466, 695, 705, 1078, 1621
BseDI	CCNNGG	7 cut(s)	175, 464, 694, 808, 1619, 2244, 2898
BseJI	GATNNNNATC	1 cut(s)	1915
BseLI	CCNNNNNNNGG	9 cut(s)	83, 357, 358, 413, 781, 1578, 2018, 2022, 2666
BseMI	GCAATG	3 cut(s)	126, 266, 1058
BseNI	ACTGG	7 cut(s)	10, 982, 1417, 1582, 2023, 2117, 2132
BseRI	GAGGAG	1 cut(s)	2570
BseXI	GCAGC	9 cut(s)	183, 385, 407, 655, 803, 994, 1366, 1831, 2045
BseYI	CCCAGC	3 cut(s)	305, 1271, 1556
BsgI	GTGCAG	2 cut(s)	108, 1257
BshFI	GGCC	7 cut(s)	469, 708, 932, 1679, 1915, 2243, 2897
BshNI	GGYRCC	3 cut(s)	73, 1502, 1738
BsiHKAI	GWGCWC	2 cut(s)	1406, 2477
BsiHKCI	CYCGRG	2 cut(s)	175, 1498
BsiSI	CCGG	5 cut(s)	176, 827, 2366, 2874, 2898
BslFI	GGGAC	1 cut(s)	2833
BslI	CCNNNNNNNGG	9 cut(s)	83, 357, 358, 413, 781, 1578, 2018, 2022, 2666
BsmAI	GTCTC	5 cut(s)	724, 1603, 1838, 2389, 2472
BsmFI	GGGAC	1 cut(s)	2833
BsmI	GAATGC	1 cut(s)	1609
BsnI	GGCC	7 cut(s)	469, 708, 932, 1679, 1915, 2243, 2897
Bso31I	GGTCTC	1 cut(s)	2389
BsoBI	CYCGRG	2 cut(s)	175, 1498
Bsp1286I	GDGCHC	2 cut(s)	1406, 2477
Bsp13I	TCCGGA	1 cut(s)	2365
BspACI	CCGC	2 cut(s)	197, 200
BspANI	GGCC	7 cut(s)	469, 708, 932, 1679, 1915, 2243, 2897
BspEI	TCCGGA	1 cut(s)	2365
BspHI	TCATGA	2 cut(s)	388, 1309
BspLI	GGNNCC	7 cut(s)	75, 561, 1504, 1570, 1740, 1904, 2226
BspMAI	CTGCAG	2 cut(s)	673, 1892
BspMI	ACCTGC	2 cut(s)	1353, 1750
BspQI	GCTCTTC	1 cut(s)	2482
BspT107I	GGYRCC	3 cut(s)	73, 1502, 1738
BspTI	CTTAAG	1 cut(s)	589
BspTNI	GGTCTC	1 cut(s)	2389
BsrDI	GCAATG	3 cut(s)	126, 266, 1058
BsrFI	RCCGGY	1 cut(s)	826
BsrI	ACTGG	7 cut(s)	10, 982, 1417, 1582, 2023, 2117, 2132
BssAI	RCCGGY	1 cut(s)	826
BssECI	CCNNGG	7 cut(s)	175, 464, 694, 808, 1619, 2244, 2898
BssNI	GRCGYC	1 cut(s)	365
BssT1I	CCWWGG	1 cut(s)	2244
Bst2UI	CCWGG	5 cut(s)	466, 695, 705, 1078, 1621
Bst4CI	ACNGT	5 cut(s)	260, 455, 1537, 1966, 2682
Bst6I	CTCTTC	5 cut(s)	1117, 1693, 2184, 2244, 2482
BstACI	GRCGYC	1 cut(s)	365
BstAFI	CTTAAG	1 cut(s)	589
BstAPI	GCANNNNNTGC	1 cut(s)	1173
BstBAI	YACGTR	2 cut(s)	1130, 2825
BstC8I	GCNNGC	4 cut(s)	1230, 1360, 2100, 2330
BstDSI	CCRYGG	1 cut(s)	808
BstMAI	GTCTC	5 cut(s)	724, 1603, 1838, 2389, 2472
BstMWI	GCNNNNNNNGC	8 cut(s)	613, 938, 1173, 1277, 1385, 1553, 2240, 2874
BstNI	CCWGG	5 cut(s)	466, 695, 705, 1078, 1621
BstNSI	RCATGY	4 cut(s)	118, 791, 1232, 1236
BstSCI	CCNGG	8 cut(s)	174, 175, 464, 693, 703, 1076, 1619, 2897
BstSFI	CTRYAG	3 cut(s)	669, 1811, 1888
BstV1I	GCAGC	9 cut(s)	183, 385, 407, 655, 803, 994, 1366, 1831, 2045
BstV2I	GAAGAC	1 cut(s)	39
BstX2I	RGATCY	3 cut(s)	1884, 1902, 2903
BstXI	CCANNNNNNTGG	1 cut(s)	477
BstYI	RGATCY	3 cut(s)	1884, 1902, 2903
Bsu36I	CCTNAGG	2 cut(s)	776, 2571
BsuI	GTATCC	1 cut(s)	216
BsuRI	GGCC	7 cut(s)	469, 708, 932, 1679, 1915, 2243, 2897
BtgI	CCRYGG	1 cut(s)	808
BtgZI	GCGATG	1 cut(s)	1226
BtsI	GCAGTG	1 cut(s)	96
BtsIMutI	CAGTG	7 cut(s)	96, 989, 1410, 1542, 1575, 2016, 2779
BveI	ACCTGC	2 cut(s)	1353, 1750
Cac8I	GCNNGC	4 cut(s)	1230, 1360, 2100, 2330
CciI	TCATGA	2 cut(s)	388, 1309
Cfr10I	RCCGGY	1 cut(s)	826
Cfr13I	GGNCC	3 cut(s)	805, 931, 954
Cfr9I	CCCGGG	1 cut(s)	175
CseI	GACGC	2 cut(s)	354, 2500
Csp6I	GTAC	8 cut(s)	687, 772, 882, 1127, 1503, 1779, 1967, 2566
CviQI	GTAC	8 cut(s)	687, 772, 882, 1127, 1503, 1779, 1967, 2566
DraIII	CACNNNGTG	1 cut(s)	2828
EaeI	YGGCCR	3 cut(s)	706, 1913, 2895
Eam1104I	CTCTTC	5 cut(s)	1117, 1693, 2184, 2244, 2482
EarI	CTCTTC	5 cut(s)	1117, 1693, 2184, 2244, 2482
Ecl136II	GAGCTC	1 cut(s)	2475
Eco130I	CCWWGG	1 cut(s)	2244
Eco24I	GRGCYC	1 cut(s)	2477
Eco31I	GGTCTC	1 cut(s)	2389
Eco47I	GGWCC	2 cut(s)	805, 954
Eco53kI	GAGCTC	1 cut(s)	2475
Eco57I	CTGAAG	2 cut(s)	300, 920
Eco81I	CCTNAGG	2 cut(s)	776, 2571
Eco88I	CYCGRG	2 cut(s)	175, 1498
EcoICRI	GAGCTC	1 cut(s)	2475
EcoO109I	RGGNCCY	1 cut(s)	931
EcoRI	GAATTC	1 cut(s)	1550
EcoRII	CCWGG	5 cut(s)	464, 693, 703, 1076, 1619
EcoT14I	CCWWGG	1 cut(s)	2244
EcoT22I	ATGCAT	4 cut(s)	1169, 1178, 1234, 2526
EcoT38I	GRGCYC	1 cut(s)	2477
ErhI	CCWWGG	1 cut(s)	2244
FalI	AAGNNNNNCTT	2 cut(s)	994, 1026
FaqI	GGGAC	1 cut(s)	2833
FauNDI	CATATG	1 cut(s)	472
FbaI	TGATCA	1 cut(s)	577
FblI	GTMKAC	1 cut(s)	2042
FriOI	GRGCYC	1 cut(s)	2477
FspBI	CTAG	4 cut(s)	690, 1346, 1718, 2741
GsaI	CCCAGC	3 cut(s)	309, 1275, 1560
GsuI	CTGGAG	3 cut(s)	42, 72, 2040
HaeIII	GGCC	7 cut(s)	469, 708, 932, 1679, 1915, 2243, 2897
HapII	CCGG	5 cut(s)	176, 827, 2366, 2874, 2898
HgaI	GACGC	2 cut(s)	354, 2500
Hin1I	GRCGYC	1 cut(s)	365
HincII	GTYRAC	3 cut(s)	369, 1665, 2407
HindII	GTYRAC	3 cut(s)	369, 1665, 2407
HpaI	GTTAAC	1 cut(s)	1665
HpaII	CCGG	5 cut(s)	176, 827, 2366, 2874, 2898
HphI	GGTGA	8 cut(s)	226, 587, 1066, 1115, 1427, 1948, 2177, 2773
Hpy166II	GTNNAC	7 cut(s)	369, 660, 738, 1665, 2043, 2261, 2407
Hpy8I	GTNNAC	7 cut(s)	369, 660, 738, 1665, 2043, 2261, 2407
Hpy99I	CGWCG	1 cut(s)	2539
HpyCH4III	ACNGT	5 cut(s)	260, 455, 1537, 1966, 2682
HpyCH4IV	ACGT	4 cut(s)	1129, 1640, 2215, 2824
HpyF10VI	GCNNNNNNNGC	8 cut(s)	613, 938, 1173, 1277, 1385, 1553, 2240, 2874
HpySE526I	ACGT	4 cut(s)	1129, 1640, 2215, 2824
Hsp92I	GRCGYC	1 cut(s)	365
Kpn2I	TCCGGA	1 cut(s)	2365
KpnI	GGTACC	1 cut(s)	1506
Ksp22I	TGATCA	1 cut(s)	577
KspAI	GTTAAC	1 cut(s)	1665
LguI	GCTCTTC	1 cut(s)	2482
LmnI	GCTCC	7 cut(s)	53, 187, 229, 1401, 1574, 2547, 2557
Lsp1109I	GCAGC	9 cut(s)	183, 385, 407, 655, 803, 994, 1366, 1831, 2045
MaeI	CTAG	4 cut(s)	690, 1346, 1718, 2741
MaeII	ACGT	4 cut(s)	1129, 1640, 2215, 2824
MaeIII	GTNAC	4 cut(s)	274, 326, 480, 755
MfeI	CAATTG	2 cut(s)	816, 2310
MflI	RGATCY	3 cut(s)	1884, 1902, 2903
MhlI	GDGCHC	2 cut(s)	1406, 2477
MlsI	TGGCCA	1 cut(s)	1915
MluNI	TGGCCA	1 cut(s)	1915
MlyI	GAGTC	5 cut(s)	533, 1719, 1840, 1869, 2440
MmeI	TCCRAC	3 cut(s)	1596, 2166, 2252
Mox20I	TGGCCA	1 cut(s)	1915
Mph1103I	ATGCAT	4 cut(s)	1169, 1178, 1234, 2526
MroI	TCCGGA	1 cut(s)	2365
MscI	TGGCCA	1 cut(s)	1915
MseI	TTAA	9 cut(s)	120, 486, 590, 1013, 1487, 1529, 1664, 2676, 2832
MslI	CAYNNNNRTG	6 cut(s)	387, 443, 475, 553, 1233, 2273
Msp20I	TGGCCA	1 cut(s)	1915
MspA1I	CMGCKG	1 cut(s)	398
MspCI	CTTAAG	1 cut(s)	589
MspI	CCGG	5 cut(s)	176, 827, 2366, 2874, 2898
MspR9I	CCNGG	8 cut(s)	176, 177, 466, 695, 705, 1078, 1621, 2899
MunI	CAATTG	2 cut(s)	816, 2310
Mva1269I	GAATGC	1 cut(s)	1609
MvaI	CCWGG	5 cut(s)	466, 695, 705, 1078, 1621
MwoI	GCNNNNNNNGC	8 cut(s)	613, 938, 1173, 1277, 1385, 1553, 2240, 2874
NciI	CCSGG	3 cut(s)	176, 177, 2899
NdeI	CATATG	1 cut(s)	472
NlaIV	GGNNCC	7 cut(s)	75, 561, 1504, 1570, 1740, 1904, 2226
NmuCI	GTSAC	2 cut(s)	274, 480
NsiI	ATGCAT	4 cut(s)	1169, 1178, 1234, 2526
NspI	RCATGY	4 cut(s)	118, 791, 1232, 1236
PaeI	GCATGC	1 cut(s)	1232
PagI	TCATGA	2 cut(s)	388, 1309
PaqCI	CACCTGC	1 cut(s)	1750
PciI	ACATGT	1 cut(s)	114
PciSI	GCTCTTC	1 cut(s)	2482
PctI	GAATGC	1 cut(s)	1609
PfeI	GAWTC	7 cut(s)	149, 374, 1081, 1628, 1939, 1973, 2294
PflFI	GACNNNGTC	2 cut(s)	1537, 1639
PflMI	CCANNNNNTGG	2 cut(s)	83, 2018
PleI	GAGTC	5 cut(s)	532, 1718, 1839, 1868, 2439
PpsI	GAGTC	5 cut(s)	532, 1718, 1839, 1868, 2439
Ppu21I	YACGTR	2 cut(s)	1130, 2825
PscI	ACATGT	1 cut(s)	114
PsiI	TTATAA	2 cut(s)	1692, 2628
Psp124BI	GAGCTC	1 cut(s)	2477
Psp6I	CCWGG	5 cut(s)	464, 693, 703, 1076, 1619
PspFI	CCCAGC	3 cut(s)	305, 1271, 1556
PspGI	CCWGG	5 cut(s)	464, 693, 703, 1076, 1619
PspN4I	GGNNCC	7 cut(s)	75, 561, 1504, 1570, 1740, 1904, 2226
PspPI	GGNCC	3 cut(s)	805, 931, 954
PstI	CTGCAG	2 cut(s)	673, 1892
PsuI	RGATCY	3 cut(s)	1884, 1902, 2903
PsyI	GACNNNGTC	2 cut(s)	1537, 1639
PvuII	CAGCTG	1 cut(s)	398
RsaI	GTAC	8 cut(s)	688, 773, 883, 1128, 1504, 1780, 1968, 2567
RsaNI	GTAC	8 cut(s)	687, 772, 882, 1127, 1503, 1779, 1967, 2566
RseI	CAYNNNNRTG	6 cut(s)	387, 443, 475, 553, 1233, 2273
SacI	GAGCTC	1 cut(s)	2477
SapI	GCTCTTC	1 cut(s)	2482
SaqAI	TTAA	9 cut(s)	120, 486, 590, 1013, 1487, 1529, 1664, 2676, 2832
Sau96I	GGNCC	3 cut(s)	805, 931, 954
SchI	GAGTC	5 cut(s)	533, 1719, 1840, 1869, 2440
ScrFI	CCNGG	8 cut(s)	176, 177, 466, 695, 705, 1078, 1621, 2899
SduI	GDGCHC	2 cut(s)	1406, 2477
SfcI	CTRYAG	3 cut(s)	669, 1811, 1888
SinI	GGWCC	2 cut(s)	805, 954
SmaI	CCCGGG	1 cut(s)	177
SmiMI	CAYNNNNRTG	6 cut(s)	387, 443, 475, 553, 1233, 2273
SmlI	CTYRAG	5 cut(s)	589, 1801, 1835, 2732, 2798
SmoI	CTYRAG	5 cut(s)	589, 1801, 1835, 2732, 2798
SpeI	ACTAGT	1 cut(s)	1345
SphI	GCATGC	1 cut(s)	1232
SsiI	CCGC	2 cut(s)	197, 200
SspMI	CTAG	4 cut(s)	690, 1346, 1718, 2741
SstI	GAGCTC	1 cut(s)	2477
StyD4I	CCNGG	8 cut(s)	174, 175, 464, 693, 703, 1076, 1619, 2897
StyI	CCWWGG	1 cut(s)	2244
TaaI	ACNGT	5 cut(s)	260, 455, 1537, 1966, 2682
TaiI	ACGT	4 cut(s)	1132, 1643, 2218, 2827
TaqI	TCGA	9 cut(s)	101, 650, 1135, 1601, 1713, 2537, 2603, 2663, 2845
TatI	WGTACW	3 cut(s)	881, 1778, 1966
TauI	GCSGC	2 cut(s)	200, 203
TfiI	GAWTC	7 cut(s)	149, 374, 1081, 1628, 1939, 1973, 2294
Tru1I	TTAA	9 cut(s)	120, 486, 590, 1013, 1487, 1529, 1664, 2676, 2832
Tru9I	TTAA	9 cut(s)	120, 486, 590, 1013, 1487, 1529, 1664, 2676, 2832
TscAI	CASTG	7 cut(s)	96, 989, 1417, 1542, 1582, 2023, 2779
TseFI	GTSAC	2 cut(s)	274, 480
TseI	GCWGC	9 cut(s)	171, 395, 398, 668, 791, 1007, 1379, 1844, 2033
Tsp45I	GTSAC	2 cut(s)	274, 480
TspGWI	ACGGA	1 cut(s)	797
TspMI	CCCGGG	1 cut(s)	175
TspRI	CASTG	7 cut(s)	96, 989, 1417, 1542, 1582, 2023, 2779
Tth111I	GACNNNGTC	2 cut(s)	1537, 1639
Van91I	CCANNNNNTGG	2 cut(s)	83, 2018
Vha464I	CTTAAG	1 cut(s)	589
VpaK11BI	GGWCC	2 cut(s)	805, 954
XapI	RAATTY	3 cut(s)	142, 540, 1550
XbaI	TCTAGA	1 cut(s)	2740
XceI	RCATGY	4 cut(s)	118, 791, 1232, 1236
XcmI	CCANNNNNNNNNTGG	1 cut(s)	17
XmaI	CCCGGG	1 cut(s)	175
XmiI	GTMKAC	1 cut(s)	2042
XspI	CTAG	4 cut(s)	690, 1346, 1718, 2741
Zsp2I	ATGCAT	4 cut(s)	1169, 1178, 1234, 2526

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.