Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0003599

Physical Location & Seq

Genomic Coordinates Forward (+)

55592758 .. 55595739

Gene Structure 2982 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00122460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2982 bp

ATGGAATACCAGTTTTCGTCTGGAGAGGGGAAGGATCAGGGGTCGTCTTCTGGAGCTGAGGTGGAGGGTTTGGTGCCACTGGATGGTGAAGACGAAAGCTGGAGGGATCAAAGGTCGTCGTCTTCTGGAGCTGGGGTGGAGGCTTTGGTGCCACTTGATGGTGAAGTGAGGAACTCGATTTCGGAGGATATGTTTAACAACATTGCTGAGTTTATGAATTTCGATTCTTATGCTGGATGGTGCAGCAGCCCGGGAACAATGGGGCAGATTGCGGCGGCGTTTCCATCGGTGAGTTATGCTCCTTTGAATGCCTTCAACTTCACACAACAGAACGGTGGAACATTGCCTGTGACCGAAGATGGTGGGACCTTTGATGAGGCGGGGAGTTCTTTTGATTGTGGTGACAAGATTGTGTTTCAGCAAACCCAATACGGGGCGTCAACTGGTTCCCACCACGCTCATGATGCAGCTGCTAAACCGAAGAATGGTTCTTTTCAACAGAATAATGTCATGGATATGGCAGACTGTGTGATCCCCAGGCCATATGGATTGTCACTTAATGAGAAGATGCTAAAGGCATTGTCATTTTTCAAGGAGTCTACTGGTGGGGGAATTTTGGCACAAGTATGGGTTCCCATGAAGCATGGTGATCGCTCCTTCTTAAGCACTTGCGAACAGCCCTATTTGCTTGACCATGTTCTTGCAGGGTATCGGGAAGTGTCGAGGATGTTTACATTCGCTGCAGAAGAAAAGCAGGGTACTAGCCTGGGGCTTCCTGGCCGTGTATTTGTCTCCAAGGTTCCAGAGTGGACATCAAATGTTAGCTATTACAACAAGGACGAGTACCTGAGGGTGGAACATGCAGCTAATCATCAGGTCCGTGGATCAATTGCTTTGCCGGTTTTTGATCTGAACTCTGAGATGTCATGTTGTGGTGTGTTTGAACTTGTCAGTACAAAAGATAAACTCAACTTTGATGCAGAGATGGAGATGGTTTGTGAGGCCCTCCAGGCTGTGAAGTTGAGGACCACTACGCCTCCCCGACTTCATCCCCAGTGCCTCTCAAAGAACCAAAGGGCTGCTTTAACTGAAATTACTGATGTCTTACGAGCTGTGTGTCATGCACATACATTGCCATTGGCTCTCACCTGGATTCCTTGTTGCTACTCTGAGGGTGATGCAGAGGAAATTAGAAGAGTACGTGTTCGAGCGGGTATTACAAAATCAAATGAAAAATGCATACTATGCATTGAGGAAACAGCTTGTTATATAAATGATAGAGCGATGCAAGGCTTTGTGCATGCATGTGCAGAACATCATCTTGAGGAAGGAAATGGTATTGCTGGGAAAGCACTTCAATCAAATCATCCCTTCTTCATTCATGATGTGAAGGTGTATGACATATATGACTATCCACTAGTTCATCATGCACGCAGGTATGGTTTGAACGCTGCTGTTGCAATCAGACTAAGGAGCACCTACACTGGTGATGATGACTATATCTTAGAGTTTTTTCTGCCTGTCAATATGAAGGGCAGTTCTGAACAGCAACTTTTGTTAAACAACCTCTCGGGTACCATGCAGAGAATTAGTAAGAGTTTAAGGACAGTGTCAGATGCAGAATTGGCTGGGGTAGAAGGCTCCAACACTGGGTTTCAAAGGGAGGCAGTCTCGAATGCCCCACCAATCCCCAGGAGAGATTCTCAGACAATGTCATCAGATAGTGGAATGAAGTTAACTGAGAAGATGCCTTCAAATGGTTATAATAGAAGAGATGGTGGAGTCAAAATAGATTTTCCTTCTGAAAAGGCACCACTTGGATCAAGAAAGCAGGTGGAGAAAAAGCGTAGTACAGCAGAGAAAAATGTGAGCTTGAGTGTTCTACAGCAATACTTCTCTGGGAGTCTCAAGGATGCTGCAAAAAGCATTGGAGTCTGCCCCACTACACTAAAAAGGATCTGCAGACAACACGGGATCTCAAGATGGCCATCTCGTAAAATAAATAAGGTGAATCGTTCCTTGAAGAAAATACAAACTGTACTTGATTCTGTTCAGGGGGTGGAAGGAGGATTGAAGTATGACCCAACCACTGGAGGGTTTGTGGCAACAGGGTCTACCATCCAAGAGTTTGATGCACAGAAAAATCTTTTCTTCTCTGAGAAGAACTTGCCTGCTCAGAATATTGTTCCAGTTCCCGAATATCCAGTTTCAGTACCTTCAATGTTTTGTAATGATGGTGAGAGTTTGGATATTATGTTGGAAGAGGATGGCTGTTGTATGAATGAACGTATGTTCATTCCAACTGCTCATCAGGCTAAGGAAGAGGTAAGTAAACAACACATCCCTGTGGTTGATTGTAGTATGGATTCCAAGCCGATTGCAATTGATTTTGGATCATGCCAGCCGACTGACCATGACGTCCTGCCTCATACTTGTTCGGAAAGCGCTTTTGGGGTCTCATATTTGGTAAAAGAAGTCAACAAATGGGATCAAAGCAAGGGGAATCCAAAATTAGAGAGTTCTGACTGCCATGTTATGTCTCAGAGCTCTTCTTCAAGCGTGACGCACTCATCAAATAGTTCTGGTTCAATGATGCATGGTTGTTCATCGAGTTCTCAGACCTTTGAGGAGCGAAAGTACCCTAAAGTCAAAGCAAGATATGATGAGAATGGATCGAAAATCATTGTGAAAGCTACTTATAAAGAAGATACAATTAGGTTCAAGTTTGAGCCTTCGGGTGGATGTCTTAAACTGTATGAAGAAGTTGCAAAGAGGTTGAAGTTGCAAGATGGAACTTTCCAGCTCAAGTATCTAGATGATGAACAAGAATGGGTGATGTTAGTCAGTGATGCAGATTTGCAGGAGTGCCTTGAGATATTGGATGACATTGGGACACGTAGTGTTAAGTTTATGGTTCGTGATATACCTTTTGATCTAAGCAGTTCCGGCAGTAGTAATTGTTTTCTGGCCAGGGGATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PB1 RWP-RK

Protein Analysis

993

Amino Acids

109.55

Weight (kDa)

5.55

Isoelectric Point (pI)

48.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GAF_NLP	PF22922	352 - 535	2.2e-75	NLP protein GAF domain
RWP-RK	PF02042	623 - 669	2.6e-22	RWP-RK domain
PB1	PF00564	895 - 973	6.3e-19	PB1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT3G59580 AT3G59580 AT3G59580
fragaria_vesca	FvH4_1g24430 FvH4_1g24430 FvH4_1g24430
malus_domestica	MD04G1116200.v1.1 MD12G1131800.v1.1
prunus_persica	Prupe.1G200100_v2.0.a1
pyrus_communis	pycom04g10620 pycom12g12770
rosa_chinensis	RchiOBHm_Chr2g0121311
rosa_laevigata	RLG00000018617
rosa_multiflora	Rmu_sc0000079.1_g000028 Rmu_sc0000315.1_g000101
rosa_roxburghii	Rroxscaffold_2G00122460
rosa_rugosa	Rorug02G0233300 Rorug02G0233400 Rorug02G0233500
rosa_samantha	Rh2AG290100 Rh2BG301000 Rh2CG279700 Rh2DG314900
rosa_wichuraiana	Rw2G023260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1764, 2700
AarI	CACCTGC	1 cut(s)	1822
AasI	GACNNNNNNGTC	1 cut(s)	2417
AatII	GACGTC	1 cut(s)	2421
Acc36I	ACCTGC	2 cut(s)	1425, 1822
Acc65I	GGTACC	1 cut(s)	1574
AccB1I	GGYRCC	4 cut(s)	73, 148, 1574, 1810
AccB7I	CCANNNNNTGG	3 cut(s)	83, 158, 2090
AccBSI	CCGCTC	1 cut(s)	1211
AccI	GTMKAC	2 cut(s)	599, 2114
AciI	CCGC	4 cut(s)	272, 275, 380, 1211
AcoI	YGGCCR	3 cut(s)	778, 1985, 2967
AcsI	RAATTY	2 cut(s)	217, 612
AcyI	GRCGYC	2 cut(s)	437, 2418
AdeI	CACNNNGTG	1 cut(s)	2900
AfaI	GTAC	9 cut(s)	760, 845, 955, 1200, 1576, 1852, 2040, 2214, 2639
AfeI	AGCGCT	1 cut(s)	2446
AflII	CTTAAG	1 cut(s)	661
AflIII	ACRYGT	2 cut(s)	1201, 2894
AhlI	ACTAGT	1 cut(s)	1417
AjnI	CCWGG	7 cut(s)	536, 765, 775, 1008, 1148, 1691, 2969
AjuI	GAANNNNNNNTTGG	2 cut(s)	2433, 2465
Alw21I	GWGCWC	2 cut(s)	1478, 2549
Alw26I	GTCTC	5 cut(s)	796, 1675, 1910, 2461, 2544
Ama87I	CYCGRG	2 cut(s)	250, 1570
Aor51HI	AGCGCT	1 cut(s)	2446
AoxI	GGCC	5 cut(s)	539, 778, 1002, 1985, 2967
ApeKI	GCWGC	9 cut(s)	243, 246, 467, 470, 740, 863, 1079, 1451, 1916
ApoI	RAATTY	2 cut(s)	217, 612
Asp700I	GAANNNNTTC	1 cut(s)	311
Asp718I	GGTACC	1 cut(s)	1574
AspLEI	GCGC	1 cut(s)	2447
AspS9I	GGNCC	4 cut(s)	366, 877, 1003, 1026
AsuC2I	CCSGG	2 cut(s)	251, 252
AvaI	CYCGRG	2 cut(s)	250, 1570
AvaII	GGWCC	3 cut(s)	366, 877, 1026
AxyI	CCTNAGG	1 cut(s)	848
BalI	TGGCCA	2 cut(s)	1987, 2969
BanI	GGYRCC	4 cut(s)	73, 148, 1574, 1810
BanII	GRGCYC	1 cut(s)	2549
BbsI	GAAGAC	3 cut(s)	39, 96, 114
Bbv12I	GWGCWC	2 cut(s)	1478, 2549
BbvCI	CCTCAGC	1 cut(s)	57
BbvI	GCAGC	9 cut(s)	255, 258, 457, 479, 727, 875, 1066, 1438, 1903
BceAI	ACGGC	1 cut(s)	765
BcgI	CGANNNNNNTGC	4 cut(s)	212, 246, 632, 666
BciT130I	CCWGG	7 cut(s)	538, 767, 777, 1010, 1150, 1693, 2971
BcnI	CCSGG	2 cut(s)	251, 252
BcoDI	GTCTC	5 cut(s)	796, 1675, 1910, 2461, 2544
BcuI	ACTAGT	1 cut(s)	1417
BfaI	CTAG	3 cut(s)	762, 1418, 2813
BfmI	CTRYAG	3 cut(s)	741, 1883, 1960
BfoI	RGCGCY	1 cut(s)	2448
BfrI	CTTAAG	1 cut(s)	661
BfuAI	ACCTGC	2 cut(s)	1425, 1822
BglI	GCCNNNNNGGC	1 cut(s)	1010
Bme1390I	CCNGG	9 cut(s)	251, 252, 538, 767, 777, 1010, 1150, 1693, 2971
Bme18I	GGWCC	3 cut(s)	366, 877, 1026
BmeT110I	CYCGRG	2 cut(s)	250, 1570
BmgT120I	GGNCC	4 cut(s)	366, 877, 1003, 1026
BmiI	GGNNCC	9 cut(s)	75, 150, 367, 448, 633, 801, 1576, 1642, 1812
BmrFI	CCNGG	9 cut(s)	251, 252, 538, 767, 777, 1010, 1150, 1693, 2971
BmrI	ACTGGG	2 cut(s)	1048, 1659
BmuI	ACTGGG	2 cut(s)	1048, 1659
BpiI	GAAGAC	3 cut(s)	39, 96, 114
BplI	GAGNNNNNCTC	6 cut(s)	283, 315, 1655, 1687, 1687, 1719
BpmI	CTGGAG	6 cut(s)	42, 72, 121, 147, 992, 2112
Bpu10I	CCTNAGC	2 cut(s)	57, 2316
BpuEI	CTTGAG	6 cut(s)	1343, 1892, 1894, 1963, 2789, 2891
BpuMI	CCSGG	2 cut(s)	251, 252
BsaAI	YACGTR	2 cut(s)	1202, 2897
BsaBI	GATNNNNATC	1 cut(s)	1987
BsaHI	GRCGYC	2 cut(s)	437, 2418
BsaI	GGTCTC	1 cut(s)	2461
BsaJI	CCNNGG	7 cut(s)	250, 536, 766, 795, 880, 1691, 2970
Bse118I	RCCGGY	1 cut(s)	898
Bse21I	CCTNAGG	1 cut(s)	848
Bse3DI	GCAATG	3 cut(s)	201, 341, 1130
Bse8I	GATNNNNATC	1 cut(s)	1987
BseBI	CCWGG	7 cut(s)	538, 767, 777, 1010, 1150, 1693, 2971
BseDI	CCNNGG	7 cut(s)	250, 536, 766, 795, 880, 1691, 2970
BseJI	GATNNNNATC	1 cut(s)	1987
BseMI	GCAATG	3 cut(s)	201, 341, 1130
BseRI	GAGGAG	1 cut(s)	2642
BseXI	GCAGC	9 cut(s)	255, 258, 457, 479, 727, 875, 1066, 1438, 1903
BseYI	CCCAGC	3 cut(s)	131, 1343, 1628
BsgI	GTGCAG	2 cut(s)	262, 1329
BshFI	GGCC	5 cut(s)	541, 780, 1004, 1987, 2969
BshNI	GGYRCC	4 cut(s)	73, 148, 1574, 1810
BsiHKAI	GWGCWC	2 cut(s)	1478, 2549
BsiHKCI	CYCGRG	2 cut(s)	250, 1570
BsiSI	CCGG	3 cut(s)	251, 899, 2946
BslFI	GGGAC	2 cut(s)	379, 2905
BsmAI	GTCTC	5 cut(s)	796, 1675, 1910, 2461, 2544
BsmFI	GGGAC	2 cut(s)	379, 2905
BsmI	GAATGC	2 cut(s)	313, 1681
BsnI	GGCC	5 cut(s)	541, 780, 1004, 1987, 2969
Bso31I	GGTCTC	1 cut(s)	2461
BsoBI	CYCGRG	2 cut(s)	250, 1570
Bsp1286I	GDGCHC	2 cut(s)	1478, 2549
BspACI	CCGC	4 cut(s)	272, 275, 380, 1211
BspANI	GGCC	5 cut(s)	541, 780, 1004, 1987, 2969
BspHI	TCATGA	2 cut(s)	460, 1381
BspLI	GGNNCC	9 cut(s)	75, 150, 367, 448, 633, 801, 1576, 1642, 1812
BspMAI	CTGCAG	2 cut(s)	745, 1964
BspMI	ACCTGC	2 cut(s)	1425, 1822
BspQI	GCTCTTC	1 cut(s)	2554
BspT107I	GGYRCC	4 cut(s)	73, 148, 1574, 1810
BspTI	CTTAAG	1 cut(s)	661
BspTNI	GGTCTC	1 cut(s)	2461
BsrBI	CCGCTC	1 cut(s)	1211
BsrDI	GCAATG	3 cut(s)	201, 341, 1130
BsrFI	RCCGGY	1 cut(s)	898
BssAI	RCCGGY	1 cut(s)	898
BssECI	CCNNGG	7 cut(s)	250, 536, 766, 795, 880, 1691, 2970
BssNI	GRCGYC	2 cut(s)	437, 2418
BssT1I	CCWWGG	1 cut(s)	795
Bst2UI	CCWGG	7 cut(s)	538, 767, 777, 1010, 1150, 1693, 2971
Bst4CI	ACNGT	5 cut(s)	335, 527, 1609, 2038, 2754
Bst6I	CTCTTC	5 cut(s)	1189, 1765, 2256, 2316, 2554
BstACI	GRCGYC	2 cut(s)	437, 2418
BstAFI	CTTAAG	1 cut(s)	661
BstAPI	GCANNNNNTGC	1 cut(s)	1245
BstBAI	YACGTR	2 cut(s)	1202, 2897
BstC8I	GCNNGC	4 cut(s)	1302, 1432, 2172, 2402
BstDSI	CCRYGG	1 cut(s)	880
BstH2I	RGCGCY	1 cut(s)	2448
BstHHI	GCGC	1 cut(s)	2447
BstMAI	GTCTC	5 cut(s)	796, 1675, 1910, 2461, 2544
BstMWI	GCNNNNNNNGC	9 cut(s)	464, 685, 1010, 1245, 1349, 1457, 1625, 2312, 2946
BstNI	CCWGG	7 cut(s)	538, 767, 777, 1010, 1150, 1693, 2971
BstNSI	RCATGY	3 cut(s)	863, 1304, 1308
BstSCI	CCNGG	9 cut(s)	249, 250, 536, 765, 775, 1008, 1148, 1691, 2969
BstSFI	CTRYAG	3 cut(s)	741, 1883, 1960
BstV1I	GCAGC	9 cut(s)	255, 258, 457, 479, 727, 875, 1066, 1438, 1903
BstV2I	GAAGAC	3 cut(s)	39, 96, 114
BstX2I	RGATCY	3 cut(s)	1956, 1974, 2975
BstYI	RGATCY	3 cut(s)	1956, 1974, 2975
Bsu36I	CCTNAGG	1 cut(s)	848
BsuRI	GGCC	5 cut(s)	541, 780, 1004, 1987, 2969
BtgI	CCRYGG	1 cut(s)	880
BtgZI	GCGATG	1 cut(s)	1298
BtsIMutI	CAGTG	7 cut(s)	77, 1061, 1482, 1614, 1647, 2088, 2851
BveI	ACCTGC	2 cut(s)	1425, 1822
Cac8I	GCNNGC	4 cut(s)	1302, 1432, 2172, 2402
CciI	TCATGA	2 cut(s)	460, 1381
CfoI	GCGC	1 cut(s)	2447
Cfr10I	RCCGGY	1 cut(s)	898
Cfr13I	GGNCC	4 cut(s)	366, 877, 1003, 1026
Cfr9I	CCCGGG	1 cut(s)	250
CseI	GACGC	2 cut(s)	426, 2572
Csp6I	GTAC	9 cut(s)	759, 844, 954, 1199, 1575, 1851, 2039, 2213, 2638
CviQI	GTAC	9 cut(s)	759, 844, 954, 1199, 1575, 1851, 2039, 2213, 2638
DraIII	CACNNNGTG	1 cut(s)	2900
DrdI	GACNNNNNNGTC	1 cut(s)	2417
DseDI	GACNNNNNNGTC	1 cut(s)	2417
EaeI	YGGCCR	3 cut(s)	778, 1985, 2967
Eam1104I	CTCTTC	5 cut(s)	1189, 1765, 2256, 2316, 2554
EarI	CTCTTC	5 cut(s)	1189, 1765, 2256, 2316, 2554
Ecl136II	GAGCTC	1 cut(s)	2547
Eco130I	CCWWGG	1 cut(s)	795
Eco24I	GRGCYC	1 cut(s)	2549
Eco31I	GGTCTC	1 cut(s)	2461
Eco47I	GGWCC	3 cut(s)	366, 877, 1026
Eco47III	AGCGCT	1 cut(s)	2446
Eco53kI	GAGCTC	1 cut(s)	2547
Eco81I	CCTNAGG	1 cut(s)	848
Eco88I	CYCGRG	2 cut(s)	250, 1570
EcoICRI	GAGCTC	1 cut(s)	2547
EcoO109I	RGGNCCY	2 cut(s)	366, 1003
EcoRII	CCWGG	7 cut(s)	536, 765, 775, 1008, 1148, 1691, 2969
EcoT14I	CCWWGG	1 cut(s)	795
EcoT22I	ATGCAT	4 cut(s)	1241, 1250, 1306, 2598
EcoT38I	GRGCYC	1 cut(s)	2549
ErhI	CCWWGG	1 cut(s)	795
FalI	AAGNNNNNCTT	2 cut(s)	1066, 1098
FaqI	GGGAC	2 cut(s)	379, 2905
FauI	CCCGC	2 cut(s)	373, 1204
FauNDI	CATATG	1 cut(s)	544
FblI	GTMKAC	2 cut(s)	599, 2114
FriOI	GRGCYC	1 cut(s)	2549
FspBI	CTAG	3 cut(s)	762, 1418, 2813
GlaI	GCGC	1 cut(s)	2446
GsaI	CCCAGC	3 cut(s)	135, 1347, 1632
GsuI	CTGGAG	6 cut(s)	42, 72, 121, 147, 992, 2112
HaeII	RGCGCY	1 cut(s)	2448
HaeIII	GGCC	5 cut(s)	541, 780, 1004, 1987, 2969
HapII	CCGG	3 cut(s)	251, 899, 2946
HgaI	GACGC	2 cut(s)	426, 2572
HhaI	GCGC	1 cut(s)	2447
Hin1I	GRCGYC	2 cut(s)	437, 2418
Hin6I	GCGC	1 cut(s)	2445
HinP1I	GCGC	1 cut(s)	2445
HincII	GTYRAC	3 cut(s)	441, 1737, 2479
HindII	GTYRAC	3 cut(s)	441, 1737, 2479
HpaI	GTTAAC	1 cut(s)	1737
HpaII	CCGG	3 cut(s)	251, 899, 2946
Hpy166II	GTNNAC	8 cut(s)	441, 600, 732, 810, 1737, 2115, 2333, 2479
Hpy8I	GTNNAC	8 cut(s)	441, 600, 732, 810, 1737, 2115, 2333, 2479
Hpy99I	CGWCG	1 cut(s)	121
HpyCH4III	ACNGT	5 cut(s)	335, 527, 1609, 2038, 2754
HpyCH4IV	ACGT	4 cut(s)	1201, 2287, 2418, 2896
HpyF10VI	GCNNNNNNNGC	9 cut(s)	464, 685, 1010, 1245, 1349, 1457, 1625, 2312, 2946
HpySE526I	ACGT	4 cut(s)	1201, 2287, 2418, 2896
Hsp92I	GRCGYC	2 cut(s)	437, 2418
HspAI	GCGC	1 cut(s)	2445
KpnI	GGTACC	1 cut(s)	1578
KspAI	GTTAAC	1 cut(s)	1737
LguI	GCTCTTC	1 cut(s)	2554
LmnI	GCTCC	7 cut(s)	53, 128, 304, 659, 1473, 1646, 2629
Lsp1109I	GCAGC	9 cut(s)	255, 258, 457, 479, 727, 875, 1066, 1438, 1903
MaeI	CTAG	3 cut(s)	762, 1418, 2813
MaeII	ACGT	4 cut(s)	1201, 2287, 2418, 2896
MaeIII	GTNAC	4 cut(s)	349, 401, 552, 2560
MbiI	CCGCTC	1 cut(s)	1211
MfeI	CAATTG	2 cut(s)	888, 2382
MflI	RGATCY	3 cut(s)	1956, 1974, 2975
MhlI	GDGCHC	2 cut(s)	1478, 2549
MlsI	TGGCCA	2 cut(s)	1987, 2969
MluNI	TGGCCA	2 cut(s)	1987, 2969
MlyI	GAGTC	4 cut(s)	605, 1791, 1912, 1941
MmeI	TCCRAC	3 cut(s)	1668, 2238, 2324
Mox20I	TGGCCA	2 cut(s)	1987, 2969
Mph1103I	ATGCAT	4 cut(s)	1241, 1250, 1306, 2598
MroXI	GAANNNNTTC	1 cut(s)	311
MscI	TGGCCA	2 cut(s)	1987, 2969
MseI	TTAA	9 cut(s)	195, 558, 662, 1085, 1559, 1601, 1736, 2748, 2904
MslI	CAYNNNNRTG	5 cut(s)	459, 515, 625, 1305, 2345
Msp20I	TGGCCA	2 cut(s)	1987, 2969
MspA1I	CMGCKG	1 cut(s)	470
MspCI	CTTAAG	1 cut(s)	661
MspI	CCGG	3 cut(s)	251, 899, 2946
MspR9I	CCNGG	9 cut(s)	251, 252, 538, 767, 777, 1010, 1150, 1693, 2971
MunI	CAATTG	2 cut(s)	888, 2382
Mva1269I	GAATGC	2 cut(s)	313, 1681
MvaI	CCWGG	7 cut(s)	538, 767, 777, 1010, 1150, 1693, 2971
MwoI	GCNNNNNNNGC	9 cut(s)	464, 685, 1010, 1245, 1349, 1457, 1625, 2312, 2946
NciI	CCSGG	2 cut(s)	251, 252
NdeI	CATATG	1 cut(s)	544
NlaIV	GGNNCC	9 cut(s)	75, 150, 367, 448, 633, 801, 1576, 1642, 1812
NmuCI	GTSAC	4 cut(s)	349, 401, 552, 2560
NsiI	ATGCAT	4 cut(s)	1241, 1250, 1306, 2598
NspI	RCATGY	3 cut(s)	863, 1304, 1308
PaeI	GCATGC	1 cut(s)	1304
PagI	TCATGA	2 cut(s)	460, 1381
PaqCI	CACCTGC	1 cut(s)	1822
PciSI	GCTCTTC	1 cut(s)	2554
PctI	GAATGC	2 cut(s)	313, 1681
PdmI	GAANNNNTTC	1 cut(s)	311
PfeI	GAWTC	7 cut(s)	224, 1153, 1700, 2011, 2045, 2366, 2503
PflFI	GACNNNGTC	2 cut(s)	1609, 1711
PflMI	CCANNNNNTGG	3 cut(s)	83, 158, 2090
PleI	GAGTC	4 cut(s)	604, 1790, 1911, 1940
PpsI	GAGTC	4 cut(s)	604, 1790, 1911, 1940
Ppu21I	YACGTR	2 cut(s)	1202, 2897
PpuMI	RGGWCCY	1 cut(s)	366
PsiI	TTATAA	2 cut(s)	1764, 2700
Psp124BI	GAGCTC	1 cut(s)	2549
Psp5II	RGGWCCY	1 cut(s)	366
Psp6I	CCWGG	7 cut(s)	536, 765, 775, 1008, 1148, 1691, 2969
PspFI	CCCAGC	3 cut(s)	131, 1343, 1628
PspGI	CCWGG	7 cut(s)	536, 765, 775, 1008, 1148, 1691, 2969
PspN4I	GGNNCC	9 cut(s)	75, 150, 367, 448, 633, 801, 1576, 1642, 1812
PspPI	GGNCC	4 cut(s)	366, 877, 1003, 1026
PspPPI	RGGWCCY	1 cut(s)	366
PstI	CTGCAG	2 cut(s)	745, 1964
PsuI	RGATCY	3 cut(s)	1956, 1974, 2975
PsyI	GACNNNGTC	2 cut(s)	1609, 1711
PvuII	CAGCTG	1 cut(s)	470
RsaI	GTAC	9 cut(s)	760, 845, 955, 1200, 1576, 1852, 2040, 2214, 2639
RsaNI	GTAC	9 cut(s)	759, 844, 954, 1199, 1575, 1851, 2039, 2213, 2638
RseI	CAYNNNNRTG	5 cut(s)	459, 515, 625, 1305, 2345
SacI	GAGCTC	1 cut(s)	2549
SapI	GCTCTTC	1 cut(s)	2554
SaqAI	TTAA	9 cut(s)	195, 558, 662, 1085, 1559, 1601, 1736, 2748, 2904
Sau96I	GGNCC	4 cut(s)	366, 877, 1003, 1026
SchI	GAGTC	4 cut(s)	605, 1791, 1912, 1941
ScrFI	CCNGG	9 cut(s)	251, 252, 538, 767, 777, 1010, 1150, 1693, 2971
SduI	GDGCHC	2 cut(s)	1478, 2549
SfcI	CTRYAG	3 cut(s)	741, 1883, 1960
SinI	GGWCC	3 cut(s)	366, 877, 1026
SmaI	CCCGGG	1 cut(s)	252
SmiMI	CAYNNNNRTG	5 cut(s)	459, 515, 625, 1305, 2345
SmlI	CTYRAG	7 cut(s)	661, 1322, 1873, 1907, 1978, 2804, 2870
SmoI	CTYRAG	7 cut(s)	661, 1322, 1873, 1907, 1978, 2804, 2870
SpeI	ACTAGT	1 cut(s)	1417
SphI	GCATGC	1 cut(s)	1304
SsiI	CCGC	4 cut(s)	272, 275, 380, 1211
SspI	AATATT	1 cut(s)	2182
SspMI	CTAG	3 cut(s)	762, 1418, 2813
SstI	GAGCTC	1 cut(s)	2549
StyD4I	CCNGG	9 cut(s)	249, 250, 536, 765, 775, 1008, 1148, 1691, 2969
StyI	CCWWGG	1 cut(s)	795
TaaI	ACNGT	5 cut(s)	335, 527, 1609, 2038, 2754
TaiI	ACGT	4 cut(s)	1204, 2290, 2421, 2899
TaqI	TCGA	7 cut(s)	176, 222, 722, 1207, 1673, 2609, 2675
TaqII	GACCGA	1 cut(s)	368
TatI	WGTACW	3 cut(s)	953, 1850, 2038
TauI	GCSGC	2 cut(s)	275, 278
TfiI	GAWTC	7 cut(s)	224, 1153, 1700, 2011, 2045, 2366, 2503
Tru1I	TTAA	9 cut(s)	195, 558, 662, 1085, 1559, 1601, 1736, 2748, 2904
Tru9I	TTAA	9 cut(s)	195, 558, 662, 1085, 1559, 1601, 1736, 2748, 2904
TscAI	CASTG	7 cut(s)	84, 1061, 1489, 1614, 1654, 2095, 2851
TseFI	GTSAC	4 cut(s)	349, 401, 552, 2560
TseI	GCWGC	9 cut(s)	243, 246, 467, 470, 740, 863, 1079, 1451, 1916
Tsp45I	GTSAC	4 cut(s)	349, 401, 552, 2560
TspGWI	ACGGA	1 cut(s)	869
TspMI	CCCGGG	1 cut(s)	250
TspRI	CASTG	7 cut(s)	84, 1061, 1489, 1614, 1654, 2095, 2851
Tth111I	GACNNNGTC	2 cut(s)	1609, 1711
Van91I	CCANNNNNTGG	3 cut(s)	83, 158, 2090
Vha464I	CTTAAG	1 cut(s)	661
VpaK11BI	GGWCC	3 cut(s)	366, 877, 1026
XapI	RAATTY	2 cut(s)	217, 612
XbaI	TCTAGA	1 cut(s)	2812
XceI	RCATGY	3 cut(s)	863, 1304, 1308
XcmI	CCANNNNNNNNNTGG	1 cut(s)	17
XmaI	CCCGGG	1 cut(s)	250
XmiI	GTMKAC	2 cut(s)	599, 2114
XmnI	GAANNNNTTC	1 cut(s)	311
XspI	CTAG	3 cut(s)	762, 1418, 2813
ZraI	GACGTC	1 cut(s)	2419
Zsp2I	ATGCAT	4 cut(s)	1241, 1250, 1306, 2598

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.