Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003599

Physical Location & Seq

Genomic Coordinates Reverse (-)

20681503 .. 20688355

Gene Structure 6853 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1131800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2934 bp

ATGGATTATCACGTTTCGCCTAAAGATAAGGGAAATGATCACTGGGCTTCTGCCAGAGCTCAGGTTGAGAATTTGGCATCATTTGACGATGGAACAGGGAACCCGATCTCAGAGGATATGTTTAATAACATTTCTGAGCTCATGAACTTTGACACTTATGCTGGATGGTGCAGCCCAGCAGCAACGGACCAGATTGCAGCCTCTTTGGGGATGCCATCATGTCCATCAGTGACCTATACACCCTTGGATGCCTTGAATTTCGTAGGACATAATGGTGAGCAATTGCCAGGTACGAGTGGTGCGGGAACTTTCAATGTTGGGGGAAGTTTCAATTGTGGAGACAAAATTGTGTTTGAGCACGTGGGAACCCCCCAATATGGTGTTTCAACAGATTCCAATGATGCGAATGACTCAATTGTTAAGCTAAATAATGGGTCTTTTCAACAGAATAATGTCATGGGCATGGAAGACTATATGATCTATAGGCCACCTGGATTGTCACTCAATGAGAAGATGCTTAAGGCATTGTCGATGTTTAAAGAGACTTCTGGTGGGGGTATTTTAGCGCAATTATGGGTTCCCATGAAGCATGGTGATCAATACTTATTAAGCACTTGTGAGCAACCCTACTTGCTTGACCATATTCTTACAGGGTATCGTGAAGTCTCAAGGATGTTTACGTTCTCTGCAGAAGAAAAACAGGGTTCTATCTTGGGTCTTCCTGGCCGTGTATTTGTCTCCAAAACTCCAGAGTGGACTTCAAATGTTAATTTTTACAATAAGACTGAGTACCTGCGGGTGGATTATGCTGCTAATCATCAGGTTCGTGGATCAATTGCCTTACCGATTTTTGATTTTGACGTGGAAAGTTCATGTTGCGCTGTATTGGAACTTGTCAGTACAAAAGAGAAATCCAATTTCGATACAGAGATGGAAATAGTTTGCAGTGCACTTCGGGCCGTTAATTTGAAGACCAATGCGCCTCTCCGACTTCTTCCCCAGTGCCTCTCAAAGAACCAAAGAGCTGCTTTAACTGAAATAAATGATGTCTTGCGTGTTATATGCCATGCACATTTATTGCCATTGGCTATGACCTGGATTCCTTGTTGCTACTCTGAGGGAGATGATGATGGAATTAAAAGAGTACGTGTCAAAGGGGGTTTTGCGAATTCAGATGAGAAATGTATACTTTGCATCGAGGAAACAGCTTGTTATGTAAATGATAGAAGAATGCAAGGCTTTGTGCATGCATGTGCAGAACATCATCTTGAGGAAGGGGAGGGAATTGCTGGTAAAGCACTGCAATCAAATCATCCATTCTTCATTAATGATGTTAAAGCGTATAATATATATGAATATCCACTTGTTCATCATGCACGCAAGTATGGTTTGAACGCTGCTGTTGCAATTAGGCTAAGGAGCACCTACACCGGTGATGATGACTATATATTGGAGTTTTTTCTCCCTGTCAATGTGAAGGGGAGTACAGAACAACAACTTCTGTTAAACAACCTCTCAGGTACCATGCAGAGAATATGCCAGAGTTTAAGGACAGTTTCAGATGCAGAATTAGTTGGGTTAGGAAGCGCCAACACTGGGTTTCAGAAGGAGACAAGCCCTAATATCCCACAAAGAAATTTTCAGCCAACACTGTCAGATAGTGAAATGAATTCAGCTGAAAATGTGACCTTTAATGTTTTTAATCAAAGAAATGGGGGAATCGAAAGAGATAATCCTCCTAAACAGGCACCAAGTGGGTCAACAAGGCAGGCGGAGAAAAAGCGAAGTACAGCGGAGAAAAATGTAAGCTTGAGTGTTTTACAGCAGTACTTCTCTGGGAGTCTCAAGGATGCTGCAAAAAGTATAGGAGTTTGTCCCACTACGCTAAAAAGGATCTGCAGACAACATGGGATCTCAAGATGGCCATCTCGTAAAATAAATAAGGTGAACCGTTCATTGCGGAAAATACAGACGGTGCTTGATTCTGTTCAGGGGGTGGAGGGAGGATTGAAGTATGACCCAACTACTGGGGGTTTTGTGACAACAGGATCCATCATCCAAGAAGTTGATGCACCAAAAAATCTTTTATTTCCTGAGAAGAACCTGCTTGTTGAGAACTCTGAGCCAGTTGCCCGACATCCCATTTCAATGCCTTCCTACAATACTGGTGAGAGTTTGACAGTTAAGTTGGAAGAGGATGGGAGTTGTGTTCCAACTTCTCATGAGAAAGAGGTAAAGACACAGAGCATCCCTTTCATGCCTCAAGGGGACTCTAAGCCTATTGCAATGGATTTTGGATCATGCGATCCTACTAACCATGGGATCACACCTGATTCGAAGGGCTCTTATCTTGCAAAAGAAGTTAATAAATGGGGTCACATCCAAAACAGTCTAAGGTTAGACAATTCTGACTGCCACTTTGTGTCTCAGAACTCAAGCTCCTTGGTTGCTGCTGATGAGATGGACATGGGAGTACATGGAGATGATGGGATTGTTGAATATAACCAACATAGTTCTTCGAGCTTGACAGATTCATCGAATGACTTTGGTTCAACAATGCAGGGATACTCATCAAGCACTCAGAGCTTTGAGGAGCAAAAGCACCCACAAGTCGAGACAAGCACTGGAGAGAATGGGTCAAAAATAATCGTGAAAGCTACTTATAAAGAGGATACAATCAGGTTCAAGTTTGAGCCTTCTCTTGGGTGCCTTCAACTGTATGAAGAGGTTGCAAAGAGGTTGAAGTTGCAAAATGGGACATTCCAACTCAAGTATTTGGATGATGAAGAGGAATGGGTGATGTTAGTCAGCGATGCAGATTTGCAGGAGTGTCTTGAGATACTGGATGACATCGGAAAGCGTTGTGCTAAGTTCATGGTCCGTGATATACCTTCCGGCGTGGGTAGTTCTGGAAGTAGCAACTTTCTGGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PB1 RWP-RK

Protein Analysis

978

Amino Acids

107.71

Weight (kDa)

5.26

Isoelectric Point (pI)

42.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GAF_NLP	PF22922	334 - 517	1e-76	NLP protein GAF domain
RWP-RK	PF02042	602 - 648	2.6e-22	RWP-RK domain
PB1	PF00564	883 - 961	5.6e-19	PB1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT3G59580 AT3G59580 AT3G59580
fragaria_vesca	FvH4_1g24430 FvH4_1g24430 FvH4_1g24430
malus_domestica	MD04G1116200.v1.1 MD12G1131800.v1.1
prunus_persica	Prupe.1G200100_v2.0.a1
pyrus_communis	pycom04g10620 pycom12g12770
rosa_chinensis	RchiOBHm_Chr2g0121311
rosa_laevigata	RLG00000018617
rosa_multiflora	Rmu_sc0000079.1_g000028 Rmu_sc0000315.1_g000101
rosa_roxburghii	Rroxscaffold_2G00122460
rosa_rugosa	Rorug02G0233300 Rorug02G0233400 Rorug02G0233500
rosa_samantha	Rh2AG290100 Rh2BG301000 Rh2CG279700 Rh2DG314900
rosa_wichuraiana	Rw2G023260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2664
Acc36I	ACCTGC	2 cut(s)	801, 2112
Acc65I	GGTACC	1 cut(s)	1520
AccB1I	GGYRCC	3 cut(s)	1520, 1747, 2706
AccB7I	CCANNNNNTGG	1 cut(s)	2027
AccI	GTMKAC	1 cut(s)	1186
AciI	CCGC	5 cut(s)	302, 796, 1772, 1793, 1960
AclWI	GGATC	8 cut(s)	838, 1901, 1919, 2043, 2056, 2300, 2305, 2330
AcoI	YGGCCR	2 cut(s)	724, 1922
AcsI	RAATTY	5 cut(s)	70, 256, 1168, 1636, 1669
AcvI	CACGTG	1 cut(s)	361
AdeI	CACNNNGTG	2 cut(s)	1754, 2422
AfaI	GTAC	9 cut(s)	292, 791, 901, 1146, 1486, 1522, 1789, 1829, 2475
AfiI	CCNNNNNNNGG	6 cut(s)	207, 377, 799, 1596, 2027, 2702
AflII	CTTAAG	1 cut(s)	518
AflIII	ACRYGT	1 cut(s)	1147
AgeI	ACCGGT	1 cut(s)	1430
AjiI	CACGTC	1 cut(s)	862
AjnI	CCWGG	4 cut(s)	286, 490, 721, 1094
AloI	GAACNNNNNNTCC	2 cut(s)	2423, 2455
Alw21I	GWGCWC	5 cut(s)	61, 141, 360, 952, 1424
Alw26I	GTCTC	8 cut(s)	333, 536, 670, 742, 1604, 1847, 2430, 2609
Alw44I	GTGCAC	1 cut(s)	948
AlwI	GGATC	8 cut(s)	838, 1901, 1919, 2043, 2056, 2300, 2305, 2330
AoxI	GGCC	4 cut(s)	485, 724, 957, 1922
ApaLI	GTGCAC	1 cut(s)	948
ApeKI	GCWGC	8 cut(s)	171, 179, 197, 809, 1025, 1397, 1853, 2450
ApoI	RAATTY	5 cut(s)	70, 256, 1168, 1636, 1669
ArsI	GACNNNNNNTTYG	2 cut(s)	2402, 2434
AseI	ATTAAT	1 cut(s)	1326
AsiGI	ACCGGT	1 cut(s)	1430
Asp718I	GGTACC	1 cut(s)	1520
AspLEI	GCGC	4 cut(s)	568, 881, 982, 1589
AspS9I	GGNCC	3 cut(s)	187, 957, 2878
AsuHPI	GGTGA	6 cut(s)	287, 605, 1445, 1957, 2180, 2809
AsuII	TTCGAA	1 cut(s)	2336
AvaII	GGWCC	2 cut(s)	187, 2878
BaeGI	GKGCMC	1 cut(s)	952
BalI	TGGCCA	1 cut(s)	1924
BamHI	GGATCC	1 cut(s)	2048
BanI	GGYRCC	3 cut(s)	1520, 1747, 2706
BanII	GRGCYC	3 cut(s)	61, 141, 2345
BbrPI	CACGTG	1 cut(s)	361
BbsI	GAAGAC	3 cut(s)	474, 710, 977
Bbv12I	GWGCWC	5 cut(s)	61, 141, 360, 952, 1424
BbvI	GCAGC	8 cut(s)	183, 191, 209, 796, 1012, 1384, 1840, 2437
BceAI	ACGGC	2 cut(s)	711, 944
BciT130I	CCWGG	4 cut(s)	288, 492, 723, 1096
BciVI	GTATCC	2 cut(s)	2558, 2665
BclI	TGATCA	2 cut(s)	37, 595
BcoDI	GTCTC	8 cut(s)	333, 536, 670, 742, 1604, 1847, 2430, 2609
BfmI	CTRYAG	3 cut(s)	481, 687, 1897
BfoI	RGCGCY	1 cut(s)	1590
BfrI	CTTAAG	1 cut(s)	518
BfuAI	ACCTGC	2 cut(s)	801, 2112
BfuI	GTATCC	2 cut(s)	2558, 2665
BisI	GCNGC	8 cut(s)	172, 180, 198, 810, 1026, 1398, 1854, 2451
BlsI	GCNGC	8 cut(s)	173, 181, 199, 811, 1027, 1399, 1855, 2452
BmcAI	AGTACT	1 cut(s)	1829
Bme1390I	CCNGG	4 cut(s)	288, 492, 723, 1096
Bme18I	GGWCC	2 cut(s)	187, 2878
BmgBI	CACGTC	1 cut(s)	862
BmgT120I	GGNCC	3 cut(s)	187, 957, 2878
BmiI	GGNNCC	7 cut(s)	101, 367, 579, 1522, 1749, 2050, 2708
BmrFI	CCNGG	4 cut(s)	288, 492, 723, 1096
BmrI	ACTGGG	4 cut(s)	52, 994, 1605, 2037
BmuI	ACTGGG	4 cut(s)	52, 994, 1605, 2037
BpiI	GAAGAC	3 cut(s)	474, 710, 977
BpmI	CTGGAG	2 cut(s)	732, 2646
Bpu10I	CCTNAGC	2 cut(s)	60, 1415
Bpu14I	TTCGAA	1 cut(s)	2336
BpuEI	CTTGAG	9 cut(s)	652, 1289, 1829, 1831, 1900, 2247, 2419, 2753, 2855
BsaAI	YACGTR	2 cut(s)	361, 1148
BsaBI	GATNNNNATC	2 cut(s)	1924, 2569
BsaJI	CCNNGG	3 cut(s)	243, 2317, 2442
BsaWI	WCCGGW	1 cut(s)	1430
BsaXI	ACNNNNNCTCC	2 cut(s)	2423, 2453
Bsc4I	CCNNNNNNNGG	6 cut(s)	207, 377, 799, 1596, 2027, 2702
Bse118I	RCCGGY	1 cut(s)	1430
Bse1I	ACTGG	8 cut(s)	47, 1000, 1600, 2032, 2126, 2170, 2629, 2847
Bse3DI	GCAATG	2 cut(s)	1955, 2292
Bse8I	GATNNNNATC	2 cut(s)	1924, 2569
BseBI	CCWGG	4 cut(s)	288, 492, 723, 1096
BseDI	CCNNGG	3 cut(s)	243, 2317, 2442
BseJI	GATNNNNATC	2 cut(s)	1924, 2569
BseLI	CCNNNNNNNGG	6 cut(s)	207, 377, 799, 1596, 2027, 2702
BseMI	GCAATG	2 cut(s)	1955, 2292
BseNI	ACTGG	8 cut(s)	47, 1000, 1600, 2032, 2126, 2170, 2629, 2847
BseRI	GAGGAG	1 cut(s)	2606
BseSI	GKGCMC	1 cut(s)	952
BseXI	GCAGC	8 cut(s)	183, 191, 209, 796, 1012, 1384, 1840, 2437
BseYI	CCCAGC	1 cut(s)	175
BsgI	GTGCAG	2 cut(s)	190, 1275
BshFI	GGCC	4 cut(s)	487, 726, 959, 1924
BshNI	GGYRCC	3 cut(s)	1520, 1747, 2706
BshTI	ACCGGT	1 cut(s)	1430
BsiHKAI	GWGCWC	5 cut(s)	61, 141, 360, 952, 1424
BsiSI	CCGG	2 cut(s)	1431, 2895
BslFI	GGGAC	3 cut(s)	1860, 2282, 2770
BslI	CCNNNNNNNGG	6 cut(s)	207, 377, 799, 1596, 2027, 2702
BsmAI	GTCTC	8 cut(s)	333, 536, 670, 742, 1604, 1847, 2430, 2609
BsmFI	GGGAC	3 cut(s)	1860, 2282, 2770
BsmI	GAATGC	1 cut(s)	1236
BsnI	GGCC	4 cut(s)	487, 726, 959, 1924
Bsp119I	TTCGAA	1 cut(s)	2336
Bsp1286I	GDGCHC	6 cut(s)	61, 141, 360, 952, 1424, 2345
Bsp19I	CCATGG	1 cut(s)	2317
BspACI	CCGC	5 cut(s)	302, 796, 1772, 1793, 1960
BspANI	GGCC	4 cut(s)	487, 726, 959, 1924
BspHI	TCATGA	2 cut(s)	141, 2221
BspLI	GGNNCC	7 cut(s)	101, 367, 579, 1522, 1749, 2050, 2708
BspMAI	CTGCAG	2 cut(s)	691, 1901
BspMI	ACCTGC	2 cut(s)	801, 2112
BspPI	GGATC	8 cut(s)	838, 1901, 1919, 2043, 2056, 2300, 2305, 2330
BspT104I	TTCGAA	1 cut(s)	2336
BspT107I	GGYRCC	3 cut(s)	1520, 1747, 2706
BspTI	CTTAAG	1 cut(s)	518
BsrDI	GCAATG	2 cut(s)	1955, 2292
BsrFI	RCCGGY	1 cut(s)	1430
BsrI	ACTGG	8 cut(s)	47, 1000, 1600, 2032, 2126, 2170, 2629, 2847
BssAI	RCCGGY	1 cut(s)	1430
BssECI	CCNNGG	3 cut(s)	243, 2317, 2442
BssNAI	GTATAC	1 cut(s)	1187
BssT1I	CCWWGG	3 cut(s)	243, 2317, 2442
Bst1107I	GTATAC	1 cut(s)	1187
Bst2UI	CCWGG	4 cut(s)	288, 492, 723, 1096
Bst4CI	ACNGT	7 cut(s)	1555, 1653, 1952, 1975, 2182, 2390, 2718
Bst6I	CTCTTC	3 cut(s)	2187, 2718, 2781
BstAFI	CTTAAG	1 cut(s)	518
BstBAI	YACGTR	2 cut(s)	361, 1148
BstBI	TTCGAA	1 cut(s)	2336
BstC8I	GCNNGC	3 cut(s)	1248, 1378, 1770
BstDSI	CCRYGG	1 cut(s)	2317
BstH2I	RGCGCY	1 cut(s)	1590
BstHHI	GCGC	4 cut(s)	568, 881, 982, 1589
BstMAI	GTCTC	8 cut(s)	333, 536, 670, 742, 1604, 1847, 2430, 2609
BstMWI	GCNNNNNNNGC	4 cut(s)	956, 1295, 1403, 2583
BstNI	CCWGG	4 cut(s)	288, 492, 723, 1096
BstNSI	RCATGY	2 cut(s)	1250, 1254
BstSCI	CCNGG	4 cut(s)	286, 490, 721, 1094
BstSFI	CTRYAG	3 cut(s)	481, 687, 1897
BstSLI	GKGCMC	1 cut(s)	952
BstV1I	GCAGC	8 cut(s)	183, 191, 209, 796, 1012, 1384, 1840, 2437
BstV2I	GAAGAC	3 cut(s)	474, 710, 977
BstX2I	RGATCY	3 cut(s)	1893, 1911, 2048
BstYI	RGATCY	3 cut(s)	1893, 1911, 2048
BstZ17I	GTATAC	1 cut(s)	1187
BsuI	GTATCC	2 cut(s)	2558, 2665
BsuRI	GGCC	4 cut(s)	487, 726, 959, 1924
BtgI	CCRYGG	1 cut(s)	2317
BtgZI	GCGATG	1 cut(s)	2826
BtrI	CACGTC	1 cut(s)	862
BtsI	GCAGTG	2 cut(s)	952, 1298
BtsIMutI	CAGTG	8 cut(s)	40, 234, 952, 1007, 1298, 1593, 1649, 2622
BveI	ACCTGC	2 cut(s)	801, 2112
Cac8I	GCNNGC	3 cut(s)	1248, 1378, 1770
CciI	TCATGA	2 cut(s)	141, 2221
CfoI	GCGC	4 cut(s)	568, 881, 982, 1589
Cfr10I	RCCGGY	1 cut(s)	1430
Cfr13I	GGNCC	3 cut(s)	187, 957, 2878
Csp6I	GTAC	9 cut(s)	291, 790, 900, 1145, 1485, 1521, 1788, 1828, 2474
CspAI	ACCGGT	1 cut(s)	1430
CspCI	CAANNNNNGTGG	2 cut(s)	477, 512
CviQI	GTAC	9 cut(s)	291, 790, 900, 1145, 1485, 1521, 1788, 1828, 2474
DraI	TTTAAA	1 cut(s)	538
DraIII	CACNNNGTG	2 cut(s)	1754, 2422
EaeI	YGGCCR	2 cut(s)	724, 1922
Eam1104I	CTCTTC	3 cut(s)	2187, 2718, 2781
EarI	CTCTTC	3 cut(s)	2187, 2718, 2781
EciI	GGCGGA	1 cut(s)	1787
Ecl136II	GAGCTC	2 cut(s)	59, 139
Eco130I	CCWWGG	3 cut(s)	243, 2317, 2442
Eco24I	GRGCYC	3 cut(s)	61, 141, 2345
Eco47I	GGWCC	2 cut(s)	187, 2878
Eco53kI	GAGCTC	2 cut(s)	59, 139
Eco72I	CACGTG	1 cut(s)	361
EcoICRI	GAGCTC	2 cut(s)	59, 139
EcoRI	GAATTC	2 cut(s)	1168, 1669
EcoRII	CCWGG	4 cut(s)	286, 490, 721, 1094
EcoT14I	CCWWGG	3 cut(s)	243, 2317, 2442
EcoT22I	ATGCAT	1 cut(s)	1252
EcoT38I	GRGCYC	3 cut(s)	61, 141, 2345
ErhI	CCWWGG	3 cut(s)	243, 2317, 2442
FalI	AAGNNNNNCTT	2 cut(s)	1012, 1044
FaqI	GGGAC	3 cut(s)	1860, 2282, 2770
FauI	CCCGC	2 cut(s)	295, 789
FbaI	TGATCA	2 cut(s)	37, 595
FblI	GTMKAC	1 cut(s)	1186
Fnu4HI	GCNGC	8 cut(s)	172, 180, 198, 810, 1026, 1398, 1854, 2451
FriOI	GRGCYC	3 cut(s)	61, 141, 2345
Fsp4HI	GCNGC	8 cut(s)	172, 180, 198, 810, 1026, 1398, 1854, 2451
GlaI	GCGC	4 cut(s)	567, 880, 981, 1588
GluI	GCNGC	8 cut(s)	172, 180, 198, 810, 1026, 1398, 1854, 2451
GsaI	CCCAGC	1 cut(s)	179
GsuI	CTGGAG	2 cut(s)	732, 2646
HaeII	RGCGCY	1 cut(s)	1590
HaeIII	GGCC	4 cut(s)	487, 726, 959, 1924
HapII	CCGG	2 cut(s)	1431, 2895
HhaI	GCGC	4 cut(s)	568, 881, 982, 1589
Hin6I	GCGC	4 cut(s)	566, 879, 980, 1587
HinP1I	GCGC	4 cut(s)	566, 879, 980, 1587
HincII	GTYRAC	1 cut(s)	1761
HindII	GTYRAC	1 cut(s)	1761
HindIII	AAGCTT	1 cut(s)	1807
HinfI	GANTC	9 cut(s)	392, 410, 1099, 1719, 1840, 1982, 2270, 2333, 2531
HpaII	CCGG	2 cut(s)	1431, 2895
HphI	GGTGA	6 cut(s)	287, 605, 1445, 1957, 2180, 2809
Hpy166II	GTNNAC	6 cut(s)	678, 756, 950, 1187, 1761, 1948
Hpy8I	GTNNAC	6 cut(s)	678, 756, 950, 1187, 1761, 1948
HpyAV	CCTTC	8 cut(s)	1268, 1471, 1600, 2163, 2332, 2706, 2720, 2901
HpyCH4III	ACNGT	7 cut(s)	1555, 1653, 1952, 1975, 2182, 2390, 2718
HpyCH4IV	ACGT	5 cut(s)	12, 360, 680, 861, 1147
HpyF10VI	GCNNNNNNNGC	4 cut(s)	956, 1295, 1403, 2583
HpySE526I	ACGT	5 cut(s)	12, 360, 680, 861, 1147
HspAI	GCGC	4 cut(s)	566, 879, 980, 1587
KpnI	GGTACC	1 cut(s)	1524
Ksp22I	TGATCA	2 cut(s)	37, 595
LmnI	GCTCC	3 cut(s)	1419, 2444, 2593
Lsp1109I	GCAGC	8 cut(s)	183, 191, 209, 796, 1012, 1384, 1840, 2437
MaeII	ACGT	5 cut(s)	12, 360, 680, 861, 1147
MaeIII	GTNAC	5 cut(s)	229, 498, 1684, 2038, 2375
MfeI	CAATTG	4 cut(s)	281, 331, 414, 834
MflI	RGATCY	3 cut(s)	1893, 1911, 2048
MhlI	GDGCHC	6 cut(s)	61, 141, 360, 952, 1424, 2345
MlsI	TGGCCA	1 cut(s)	1924
MluNI	TGGCCA	1 cut(s)	1924
MlyI	GAGTC	3 cut(s)	404, 1849, 2264
MmeI	TCCRAC	4 cut(s)	1012, 2169, 2237, 2788
Mox20I	TGGCCA	1 cut(s)	1924
Mph1103I	ATGCAT	1 cut(s)	1252
MscI	TGGCCA	1 cut(s)	1924
MslI	CAYNNNNRTG	5 cut(s)	273, 461, 1251, 2147, 2481
Msp20I	TGGCCA	1 cut(s)	1924
MspA1I	CMGCKG	2 cut(s)	1676, 1793
MspCI	CTTAAG	1 cut(s)	518
MspI	CCGG	2 cut(s)	1431, 2895
MspR9I	CCNGG	4 cut(s)	288, 492, 723, 1096
MunI	CAATTG	4 cut(s)	281, 331, 414, 834
Mva1269I	GAATGC	1 cut(s)	1236
MvaI	CCWGG	4 cut(s)	288, 492, 723, 1096
MwoI	GCNNNNNNNGC	4 cut(s)	956, 1295, 1403, 2583
NcoI	CCATGG	1 cut(s)	2317
NlaIV	GGNNCC	7 cut(s)	101, 367, 579, 1522, 1749, 2050, 2708
NmuCI	GTSAC	5 cut(s)	229, 498, 1684, 2038, 2375
NsiI	ATGCAT	1 cut(s)	1252
NspI	RCATGY	2 cut(s)	1250, 1254
NspV	TTCGAA	1 cut(s)	2336
PaeI	GCATGC	1 cut(s)	1250
PagI	TCATGA	2 cut(s)	141, 2221
PctI	GAATGC	1 cut(s)	1236
PfeI	GAWTC	6 cut(s)	392, 1099, 1719, 1982, 2333, 2531
PflMI	CCANNNNNTGG	1 cut(s)	2027
PinAI	ACCGGT	1 cut(s)	1430
PkrI	GCNGC	8 cut(s)	173, 181, 199, 811, 1027, 1399, 1855, 2452
PleI	GAGTC	3 cut(s)	404, 1848, 2264
PmaCI	CACGTG	1 cut(s)	361
PmlI	CACGTG	1 cut(s)	361
PpsI	GAGTC	3 cut(s)	404, 1848, 2264
Ppu21I	YACGTR	2 cut(s)	361, 1148
PshBI	ATTAAT	1 cut(s)	1326
PsiI	TTATAA	1 cut(s)	2664
Psp124BI	GAGCTC	2 cut(s)	61, 141
Psp6I	CCWGG	4 cut(s)	286, 490, 721, 1094
PspCI	CACGTG	1 cut(s)	361
PspFI	CCCAGC	1 cut(s)	175
PspGI	CCWGG	4 cut(s)	286, 490, 721, 1094
PspN4I	GGNNCC	7 cut(s)	101, 367, 579, 1522, 1749, 2050, 2708
PspPI	GGNCC	3 cut(s)	187, 957, 2878
PstI	CTGCAG	2 cut(s)	691, 1901
PsuI	RGATCY	3 cut(s)	1893, 1911, 2048
PvuII	CAGCTG	1 cut(s)	1676
RsaI	GTAC	9 cut(s)	292, 791, 901, 1146, 1486, 1522, 1789, 1829, 2475
RsaNI	GTAC	9 cut(s)	291, 790, 900, 1145, 1485, 1521, 1788, 1828, 2474
RseI	CAYNNNNRTG	5 cut(s)	273, 461, 1251, 2147, 2481
SacI	GAGCTC	2 cut(s)	61, 141
SatI	GCNGC	8 cut(s)	172, 180, 198, 810, 1026, 1398, 1854, 2451
Sau96I	GGNCC	3 cut(s)	187, 957, 2878
ScaI	AGTACT	1 cut(s)	1829
SchI	GAGTC	3 cut(s)	404, 1849, 2264
ScrFI	CCNGG	4 cut(s)	288, 492, 723, 1096
SduI	GDGCHC	6 cut(s)	61, 141, 360, 952, 1424, 2345
SfcI	CTRYAG	3 cut(s)	481, 687, 1897
SfuI	TTCGAA	1 cut(s)	2336
SgrAI	CRCCGGYG	1 cut(s)	1430
SinI	GGWCC	2 cut(s)	187, 2878
SmiMI	CAYNNNNRTG	5 cut(s)	273, 461, 1251, 2147, 2481
SphI	GCATGC	1 cut(s)	1250
SsiI	CCGC	5 cut(s)	302, 796, 1772, 1793, 1960
SstI	GAGCTC	2 cut(s)	61, 141
StyD4I	CCNGG	4 cut(s)	286, 490, 721, 1094
StyI	CCWWGG	3 cut(s)	243, 2317, 2442
TaaI	ACNGT	7 cut(s)	1555, 1653, 1952, 1975, 2182, 2390, 2718
TaiI	ACGT	5 cut(s)	15, 363, 683, 864, 1150
TaqI	TCGA	8 cut(s)	530, 921, 1197, 1722, 2336, 2519, 2537, 2613
TatI	WGTACW	5 cut(s)	899, 1484, 1787, 1827, 2473
TfiI	GAWTC	6 cut(s)	392, 1099, 1719, 1982, 2333, 2531
TscAI	CASTG	8 cut(s)	47, 234, 952, 1007, 1305, 1600, 1656, 2629
TseFI	GTSAC	5 cut(s)	229, 498, 1684, 2038, 2375
TseI	GCWGC	8 cut(s)	171, 179, 197, 809, 1025, 1397, 1853, 2450
Tsp45I	GTSAC	5 cut(s)	229, 498, 1684, 2038, 2375
TspGWI	ACGGA	2 cut(s)	200, 2870
TspRI	CASTG	8 cut(s)	47, 234, 952, 1007, 1305, 1600, 1656, 2629
Van91I	CCANNNNNTGG	1 cut(s)	2027
Vha464I	CTTAAG	1 cut(s)	518
VneI	GTGCAC	1 cut(s)	948
VpaK11BI	GGWCC	2 cut(s)	187, 2878
VspI	ATTAAT	1 cut(s)	1326
XapI	RAATTY	5 cut(s)	70, 256, 1168, 1636, 1669
XceI	RCATGY	2 cut(s)	1250, 1254
XmiI	GTMKAC	1 cut(s)	1186
ZrmI	AGTACT	1 cut(s)	1829
Zsp2I	ATGCAT	1 cut(s)	1252

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.