Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0003599

Physical Location & Seq

Genomic Coordinates Reverse (-)

15183546 .. 15187881

Gene Structure 4336 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g12770.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2940 bp

ATGGATTATCACTTTTCGCCTAAAGATAAGGGAAATGATCACTGGGCTTCTGCCAGAGCTCAGGTTGAGAATTTGGCATCGTTTGACGATGGAACAAGGAACCCGATCTCAGAGGATATGTTTAATAACATTTCTGAGCTCATGAACTTTGACACTTATGCTGGATGGTGCAGCCCAGCAGCAACGGACCAGATTGCAGCCTCTTTGGGGATGCCATCATGTCCATCAGTGACCTATACACCCTTGGATGCCTTGAATCTCGTAGAACATAATGGTGAGCAATTGCCAGTTACGAGTGGTGCTGGAACTTTCAATGTTGGGGGAAGTTTCAATTGTGGAGACAAAATTGTGTTTGAGCACATGGGAACCCCCCAATATGGTGTTTCAACAGATTCCAATGATGCTAATGACTCAATTGTTAAGCTAAATAATGGGTCTTTTCAACAGAATAACGTCATGGGCATGGAAGACTATTTGATCTATAGGCCACCTGGATTGTCACTCAATGAGAAGATGCTTAAGGCATTGTCGATGTTTAAAGAGACTTCTGGTGGGGGTATTTTAGCACAATTATGGGTTCCCATGAAGCATGGTGATCAATACTTATTAAGCACTTGTGAGCAACCCTATTTGCTTGACCATATTCTTACAGGGTATCGTGAGGTCTCAAGGATGTTTACGTTCTCTGCAGAAGAAAAACAGGGTTCTATCTTGGGTCTTCCTGGCCGTGTATTTGTCTCCAAAACTCCAGAGTGGACTTCAAATGTTAGTTTTTACAATAAGACTGAGTACCTGCGGGTGGATCATGCAGCTAATCATCAGGTTCGTGGATCAATTGCCTTACCGATTTTTGATTTTGACCTGGAAAGTTCATGTTGCGCTGTATTGGAACTTGTCAGTACAAAAGAGAAACCCAATTTCGATACGGAGATGGAAATAGTTTGCAGTGCACTTCAGGCCGTTAATTTGAAGACCAATGCGCCTCTCCGACTTCTTCCCCAGTGCCTCTCAAAAAACCAAAGGGCTGCTTTAACTGAAATAAATGATGTCTTGCGTGCTATATGCCATGCACATTTATTGCCATTGGCTATGACCTGGATTCCTTGTTGCTACTCTGAGGGAGATGATGATGGAATTAAAAGAGTACGTGTCAAAGGGGGTTTTACAAATTCAGATGAGAAATGTATACTTTGCATCGAGGAAACTGCTTGTTATGTAAATGATAGAAGAATGCAAGGCTTTGTGCATGCATGTGCAGAACATCATCTTGAGGAAGGGGAGGGAATTGCTGGTAAAGCACTGCAATCAAATCATCCATTCTTCATTAATGATGTTAAAGCGTATGATATATATGAATATCCACTTGTTCATCATGCACGCAAGTATGGTTTGAACGCCGCAGTTGCAATTAGGCTAAGGAGCACCTATACCGGTGATGATGACTATATATTGGAGTTTTTTCTCCCTGTCAATGTGAAGGGGAGTACAGAACAACAACTTCTGTTAAACAACCTCTCAGGTACCATGCAGAGAATTTGTAAGAGTTTAAGGACAGTTTCAGATGCAGAATTAGTTGGGTTAGGAGGTGCCAACACTGGGTTTCAGAAGGAGACAAGCCCTAATATCCCACAAAGAAATTTTCAGACAACACTGTCAGATAGTGAAATGAATTCAGCTGAAAATGTCACCTTTAATGTTTTTAATCAAAGAAATGGGGGAATCGAAAGAGTTAATCCTCCTAAACAGGCACCGAGTGGGTCAATAAGGCAGGCGGAGAAAAAACGAAGTACAGCGGAGAAAAATGTAAGCTTGAGTGTTTTACAGCAGTACTTCTCTGGGAGTCTCAAGGATGCTGCAAAAAGTATAGGAGTTTGTCCCACTACGCTAAAAAGGATCTGCAGACAACATGGGATCTCAAGATGGCCATCTCGTAAAATAAATAAGGTGAACCGTTCATTGCGGAAAATACAGACGGTGCTTGATTCTGTTCAGGGGGTGGAGGGAGGATTGAAGTATGACCCAACTACTGGGGGGTTTGTGGCAACAGGATCCATCATCCAAGAAGTTGATGCACCAAAAAATCTTTTATTTCCTGCGAAGAACCTGCTTATTGAGAACTCTGAGCCAGTTGCCCAACATCCCATTTCAATGCCTTCAATGTCCTACAATAATGGTGAGAGTTTGACAGTTAAGTTGGAAGAGGATGGGAATTGTGTTCCAACTTCTCATGAGAAAGAGGTAAAGACACAGAACATCCCTTTCATGCCTCAAGGGGACTCTAAGCCGATTGCAATGGATTTTGGATCATGCGATCCTACTAACCATGGGATCACACCTGATTTGAAGGGCTCTTATCTTGCAAAAGAAGTTAATAAATGGGGTCACATCCAAAACAGTCTAAGGTTAGACAATTCTGACTGCCACTTTGTGTCTCAGAACTCAAGCTTCTTGGTTCCTGCTGATGAGATGGACATGGGAGTACATGGAGATGATGGGATTGTTGAATATAACCAACATAGTTCTTCGAGCTTGACGGATTCATCGAATGACTTTGGTTCAACAATGCAGGGATACTCATCAAGCACTCAGAGCTTTGAGGAGCAAAAGCACCCACAAGTCGAGACAAGCACTGCAGAGAATGGGTCAAAAATAATCGTGAAAGCTACTTATAAAGAGGATACAATCAGGTTCAAGTTTGAGCCTTCTCTTGGGTGCCTTCAACTGTACGAAGAGGTTGCAAAGAGGTTGAAGTTGCAAAATGGGACATTCCAACTCAAGTATTTGGATGATGAAGAGGAATGGGTGATGTTAGTCAGCGATGCAGATTTGCAGGAGTGTCTTGAGATACTGGATGACATCGGAAAGCGTTGTGCTAAGTTCATGGTCCGTGATATACCTTCTGGCGTGGGTAGTTCTGGAAGTAGCAACTTTCTGGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PB1 RWP-RK

Protein Analysis

980

Amino Acids

108.18

Weight (kDa)

5.29

Isoelectric Point (pI)

42.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GAF_NLP	PF22922	334 - 517	1.2e-76	NLP protein GAF domain
RWP-RK	PF02042	602 - 648	2.6e-22	RWP-RK domain
PB1	PF00564	885 - 963	5.6e-19	PB1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT3G59580 AT3G59580 AT3G59580
fragaria_vesca	FvH4_1g24430 FvH4_1g24430 FvH4_1g24430
malus_domestica	MD04G1116200.v1.1 MD12G1131800.v1.1
prunus_persica	Prupe.1G200100_v2.0.a1
pyrus_communis	pycom04g10620 pycom12g12770
rosa_chinensis	RchiOBHm_Chr2g0121311
rosa_laevigata	RLG00000018617
rosa_multiflora	Rmu_sc0000079.1_g000028 Rmu_sc0000315.1_g000101
rosa_roxburghii	Rroxscaffold_2G00122460
rosa_rugosa	Rorug02G0233300 Rorug02G0233400 Rorug02G0233500
rosa_samantha	Rh2AG290100 Rh2BG301000 Rh2CG279700 Rh2DG314900
rosa_wichuraiana	Rw2G023260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2670
AasI	GACNNNNNNGTC	1 cut(s)	1651
Acc36I	ACCTGC	2 cut(s)	801, 2112
Acc65I	GGTACC	1 cut(s)	1520
AccB1I	GGYRCC	4 cut(s)	1520, 1586, 1747, 2712
AccB7I	CCANNNNNTGG	1 cut(s)	2027
AccI	GTMKAC	1 cut(s)	1186
AciI	CCGC	5 cut(s)	796, 1398, 1772, 1793, 1960
AclWI	GGATC	9 cut(s)	810, 838, 1901, 1919, 2043, 2056, 2306, 2311, 2336
AcoI	YGGCCR	2 cut(s)	724, 1922
AcsI	RAATTY	5 cut(s)	70, 1168, 1533, 1636, 1669
AcuI	CTGAAG	1 cut(s)	938
AdeI	CACNNNGTG	2 cut(s)	1754, 2428
AfaI	GTAC	9 cut(s)	791, 901, 1146, 1486, 1522, 1789, 1829, 2481, 2726
AfiI	CCNNNNNNNGG	6 cut(s)	207, 377, 799, 1596, 2027, 2708
AflII	CTTAAG	1 cut(s)	518
AflIII	ACRYGT	1 cut(s)	1147
AgeI	ACCGGT	1 cut(s)	1430
AjnI	CCWGG	4 cut(s)	490, 721, 861, 1094
Alw21I	GWGCWC	5 cut(s)	61, 141, 360, 952, 1424
Alw26I	GTCTC	8 cut(s)	333, 536, 670, 742, 1604, 1847, 2436, 2615
Alw44I	GTGCAC	1 cut(s)	948
AlwI	GGATC	9 cut(s)	810, 838, 1901, 1919, 2043, 2056, 2306, 2311, 2336
AoxI	GGCC	4 cut(s)	485, 724, 957, 1922
ApaLI	GTGCAC	1 cut(s)	948
ApeKI	GCWGC	6 cut(s)	171, 179, 197, 809, 1025, 1853
ApoI	RAATTY	5 cut(s)	70, 1168, 1533, 1636, 1669
ArsI	GACNNNNNNTTYG	2 cut(s)	2408, 2440
AseI	ATTAAT	1 cut(s)	1326
AsiGI	ACCGGT	1 cut(s)	1430
Asp718I	GGTACC	1 cut(s)	1520
AspLEI	GCGC	2 cut(s)	881, 982
AspS9I	GGNCC	2 cut(s)	187, 2884
AsuHPI	GGTGA	7 cut(s)	287, 605, 1445, 1678, 1957, 2186, 2815
AvaII	GGWCC	2 cut(s)	187, 2884
BaeGI	GKGCMC	1 cut(s)	952
BalI	TGGCCA	1 cut(s)	1924
BamHI	GGATCC	1 cut(s)	2048
BanI	GGYRCC	4 cut(s)	1520, 1586, 1747, 2712
BanII	GRGCYC	3 cut(s)	61, 141, 2351
BbsI	GAAGAC	3 cut(s)	474, 710, 977
Bbv12I	GWGCWC	5 cut(s)	61, 141, 360, 952, 1424
BbvI	GCAGC	6 cut(s)	183, 191, 209, 821, 1012, 1840
BceAI	ACGGC	2 cut(s)	711, 944
BcgI	CGANNNNNNTGC	6 cut(s)	1187, 1221, 2086, 2120, 2717, 2751
BciT130I	CCWGG	4 cut(s)	492, 723, 863, 1096
BciVI	GTATCC	2 cut(s)	2564, 2671
BclI	TGATCA	2 cut(s)	37, 595
BcoDI	GTCTC	8 cut(s)	333, 536, 670, 742, 1604, 1847, 2436, 2615
BfmI	CTRYAG	4 cut(s)	481, 687, 1897, 2631
BfrI	CTTAAG	1 cut(s)	518
BfuAI	ACCTGC	2 cut(s)	801, 2112
BfuI	GTATCC	2 cut(s)	2564, 2671
BisI	GCNGC	7 cut(s)	172, 180, 198, 810, 1026, 1398, 1854
BlsI	GCNGC	7 cut(s)	173, 181, 199, 811, 1027, 1399, 1855
BmcAI	AGTACT	1 cut(s)	1829
Bme1390I	CCNGG	4 cut(s)	492, 723, 863, 1096
Bme18I	GGWCC	2 cut(s)	187, 2884
BmgT120I	GGNCC	2 cut(s)	187, 2884
BmiI	GGNNCC	9 cut(s)	101, 367, 579, 1522, 1588, 1749, 2050, 2454, 2714
BmrFI	CCNGG	4 cut(s)	492, 723, 863, 1096
BmrI	ACTGGG	4 cut(s)	52, 994, 1605, 2037
BmuI	ACTGGG	4 cut(s)	52, 994, 1605, 2037
BpiI	GAAGAC	3 cut(s)	474, 710, 977
BpmI	CTGGAG	1 cut(s)	732
Bpu10I	CCTNAGC	2 cut(s)	60, 1415
BpuEI	CTTGAG	9 cut(s)	652, 1289, 1829, 1831, 1900, 2253, 2425, 2759, 2861
BsaAI	YACGTR	1 cut(s)	1148
BsaBI	GATNNNNATC	2 cut(s)	1924, 2575
BsaI	GGTCTC	1 cut(s)	670
BsaJI	CCNNGG	2 cut(s)	243, 2323
BsaWI	WCCGGW	1 cut(s)	1430
Bsc4I	CCNNNNNNNGG	6 cut(s)	207, 377, 799, 1596, 2027, 2708
Bse118I	RCCGGY	1 cut(s)	1430
Bse1I	ACTGG	7 cut(s)	47, 287, 1000, 1600, 2032, 2126, 2853
Bse3DI	GCAATG	2 cut(s)	1955, 2298
Bse8I	GATNNNNATC	2 cut(s)	1924, 2575
BseBI	CCWGG	4 cut(s)	492, 723, 863, 1096
BseDI	CCNNGG	2 cut(s)	243, 2323
BseJI	GATNNNNATC	2 cut(s)	1924, 2575
BseLI	CCNNNNNNNGG	6 cut(s)	207, 377, 799, 1596, 2027, 2708
BseMI	GCAATG	2 cut(s)	1955, 2298
BseMII	CTCAG	9 cut(s)	74, 123, 126, 777, 1107, 1530, 2112, 2447, 2600
BseNI	ACTGG	7 cut(s)	47, 287, 1000, 1600, 2032, 2126, 2853
BseRI	GAGGAG	1 cut(s)	2612
BseSI	GKGCMC	1 cut(s)	952
BseXI	GCAGC	6 cut(s)	183, 191, 209, 821, 1012, 1840
BseYI	CCCAGC	1 cut(s)	175
BsgI	GTGCAG	2 cut(s)	190, 1275
BshFI	GGCC	4 cut(s)	487, 726, 959, 1924
BshNI	GGYRCC	4 cut(s)	1520, 1586, 1747, 2712
BshTI	ACCGGT	1 cut(s)	1430
BsiHKAI	GWGCWC	5 cut(s)	61, 141, 360, 952, 1424
BsiSI	CCGG	1 cut(s)	1431
BslFI	GGGAC	3 cut(s)	1860, 2288, 2776
BslI	CCNNNNNNNGG	6 cut(s)	207, 377, 799, 1596, 2027, 2708
BsmAI	GTCTC	8 cut(s)	333, 536, 670, 742, 1604, 1847, 2436, 2615
BsmFI	GGGAC	3 cut(s)	1860, 2288, 2776
BsmI	GAATGC	1 cut(s)	1236
BsnI	GGCC	4 cut(s)	487, 726, 959, 1924
Bso31I	GGTCTC	1 cut(s)	670
Bsp1286I	GDGCHC	6 cut(s)	61, 141, 360, 952, 1424, 2351
Bsp19I	CCATGG	1 cut(s)	2323
BspACI	CCGC	5 cut(s)	796, 1398, 1772, 1793, 1960
BspANI	GGCC	4 cut(s)	487, 726, 959, 1924
BspCNI	CTCAG	9 cut(s)	73, 122, 127, 778, 1108, 1529, 2113, 2446, 2599
BspHI	TCATGA	2 cut(s)	141, 2227
BspLI	GGNNCC	9 cut(s)	101, 367, 579, 1522, 1588, 1749, 2050, 2454, 2714
BspMAI	CTGCAG	3 cut(s)	691, 1901, 2635
BspMI	ACCTGC	2 cut(s)	801, 2112
BspPI	GGATC	9 cut(s)	810, 838, 1901, 1919, 2043, 2056, 2306, 2311, 2336
BspT107I	GGYRCC	4 cut(s)	1520, 1586, 1747, 2712
BspTI	CTTAAG	1 cut(s)	518
BspTNI	GGTCTC	1 cut(s)	670
BsrDI	GCAATG	2 cut(s)	1955, 2298
BsrFI	RCCGGY	1 cut(s)	1430
BsrI	ACTGG	7 cut(s)	47, 287, 1000, 1600, 2032, 2126, 2853
BssAI	RCCGGY	1 cut(s)	1430
BssECI	CCNNGG	2 cut(s)	243, 2323
BssNAI	GTATAC	1 cut(s)	1187
BssT1I	CCWWGG	2 cut(s)	243, 2323
Bst1107I	GTATAC	1 cut(s)	1187
Bst2UI	CCWGG	4 cut(s)	492, 723, 863, 1096
Bst4CI	ACNGT	7 cut(s)	1555, 1653, 1952, 1975, 2188, 2396, 2724
Bst6I	CTCTTC	3 cut(s)	2193, 2724, 2787
BstAFI	CTTAAG	1 cut(s)	518
BstBAI	YACGTR	1 cut(s)	1148
BstC8I	GCNNGC	4 cut(s)	1056, 1248, 1378, 1770
BstDSI	CCRYGG	1 cut(s)	2323
BstHHI	GCGC	2 cut(s)	881, 982
BstMAI	GTCTC	8 cut(s)	333, 536, 670, 742, 1604, 1847, 2436, 2615
BstMWI	GCNNNNNNNGC	4 cut(s)	956, 1295, 1403, 2589
BstNI	CCWGG	4 cut(s)	492, 723, 863, 1096
BstNSI	RCATGY	2 cut(s)	1250, 1254
BstSCI	CCNGG	4 cut(s)	490, 721, 861, 1094
BstSFI	CTRYAG	4 cut(s)	481, 687, 1897, 2631
BstSLI	GKGCMC	1 cut(s)	952
BstV1I	GCAGC	6 cut(s)	183, 191, 209, 821, 1012, 1840
BstV2I	GAAGAC	3 cut(s)	474, 710, 977
BstX2I	RGATCY	3 cut(s)	1893, 1911, 2048
BstYI	RGATCY	3 cut(s)	1893, 1911, 2048
BstZ17I	GTATAC	1 cut(s)	1187
BsuI	GTATCC	2 cut(s)	2564, 2671
BsuRI	GGCC	4 cut(s)	487, 726, 959, 1924
BtgI	CCRYGG	1 cut(s)	2323
BtgZI	GCGATG	1 cut(s)	2832
BtsI	GCAGTG	3 cut(s)	952, 1298, 2628
BtsIMutI	CAGTG	8 cut(s)	40, 234, 952, 1007, 1298, 1593, 1649, 2628
BveI	ACCTGC	2 cut(s)	801, 2112
Cac8I	GCNNGC	4 cut(s)	1056, 1248, 1378, 1770
CciI	TCATGA	2 cut(s)	141, 2227
CfoI	GCGC	2 cut(s)	881, 982
Cfr10I	RCCGGY	1 cut(s)	1430
Cfr13I	GGNCC	2 cut(s)	187, 2884
Csp6I	GTAC	9 cut(s)	790, 900, 1145, 1485, 1521, 1788, 1828, 2480, 2725
CspAI	ACCGGT	1 cut(s)	1430
CspCI	CAANNNNNGTGG	2 cut(s)	477, 512
CviQI	GTAC	9 cut(s)	790, 900, 1145, 1485, 1521, 1788, 1828, 2480, 2725
DraI	TTTAAA	1 cut(s)	538
DraIII	CACNNNGTG	2 cut(s)	1754, 2428
DrdI	GACNNNNNNGTC	1 cut(s)	1651
DseDI	GACNNNNNNGTC	1 cut(s)	1651
EaeI	YGGCCR	2 cut(s)	724, 1922
Eam1104I	CTCTTC	3 cut(s)	2193, 2724, 2787
EarI	CTCTTC	3 cut(s)	2193, 2724, 2787
EciI	GGCGGA	1 cut(s)	1787
Ecl136II	GAGCTC	2 cut(s)	59, 139
Eco130I	CCWWGG	2 cut(s)	243, 2323
Eco24I	GRGCYC	3 cut(s)	61, 141, 2351
Eco31I	GGTCTC	1 cut(s)	670
Eco47I	GGWCC	2 cut(s)	187, 2884
Eco53kI	GAGCTC	2 cut(s)	59, 139
Eco57I	CTGAAG	1 cut(s)	938
EcoICRI	GAGCTC	2 cut(s)	59, 139
EcoRI	GAATTC	1 cut(s)	1669
EcoRII	CCWGG	4 cut(s)	490, 721, 861, 1094
EcoT14I	CCWWGG	2 cut(s)	243, 2323
EcoT22I	ATGCAT	1 cut(s)	1252
EcoT38I	GRGCYC	3 cut(s)	61, 141, 2351
ErhI	CCWWGG	2 cut(s)	243, 2323
FalI	AAGNNNNNCTT	2 cut(s)	1012, 1044
FaqI	GGGAC	3 cut(s)	1860, 2288, 2776
FauI	CCCGC	1 cut(s)	789
FbaI	TGATCA	2 cut(s)	37, 595
FblI	GTMKAC	1 cut(s)	1186
Fnu4HI	GCNGC	7 cut(s)	172, 180, 198, 810, 1026, 1398, 1854
FriOI	GRGCYC	3 cut(s)	61, 141, 2351
Fsp4HI	GCNGC	7 cut(s)	172, 180, 198, 810, 1026, 1398, 1854
GlaI	GCGC	2 cut(s)	880, 981
GluI	GCNGC	7 cut(s)	172, 180, 198, 810, 1026, 1398, 1854
GsaI	CCCAGC	1 cut(s)	179
GsuI	CTGGAG	1 cut(s)	732
HaeIII	GGCC	4 cut(s)	487, 726, 959, 1924
HapII	CCGG	1 cut(s)	1431
HhaI	GCGC	2 cut(s)	881, 982
Hin6I	GCGC	2 cut(s)	879, 980
HinP1I	GCGC	2 cut(s)	879, 980
HindIII	AAGCTT	2 cut(s)	1807, 2443
HinfI	GANTC	9 cut(s)	256, 392, 410, 1099, 1719, 1840, 1982, 2276, 2537
HpaII	CCGG	1 cut(s)	1431
HphI	GGTGA	7 cut(s)	287, 605, 1445, 1678, 1957, 2186, 2815
Hpy166II	GTNNAC	5 cut(s)	678, 756, 950, 1187, 1948
Hpy8I	GTNNAC	5 cut(s)	678, 756, 950, 1187, 1948
HpyAV	CCTTC	8 cut(s)	1268, 1471, 1600, 2163, 2338, 2712, 2726, 2907
HpyCH4III	ACNGT	7 cut(s)	1555, 1653, 1952, 1975, 2188, 2396, 2724
HpyCH4IV	ACGT	3 cut(s)	453, 680, 1147
HpyF10VI	GCNNNNNNNGC	4 cut(s)	956, 1295, 1403, 2589
HpySE526I	ACGT	3 cut(s)	453, 680, 1147
HspAI	GCGC	2 cut(s)	879, 980
KpnI	GGTACC	1 cut(s)	1524
Ksp22I	TGATCA	2 cut(s)	37, 595
LmnI	GCTCC	2 cut(s)	1419, 2599
Lsp1109I	GCAGC	6 cut(s)	183, 191, 209, 821, 1012, 1840
MaeII	ACGT	3 cut(s)	453, 680, 1147
MaeIII	GTNAC	5 cut(s)	229, 289, 498, 1684, 2381
MfeI	CAATTG	4 cut(s)	281, 331, 414, 834
MflI	RGATCY	3 cut(s)	1893, 1911, 2048
MhlI	GDGCHC	6 cut(s)	61, 141, 360, 952, 1424, 2351
MlsI	TGGCCA	1 cut(s)	1924
MluNI	TGGCCA	1 cut(s)	1924
MlyI	GAGTC	3 cut(s)	404, 1849, 2270
MmeI	TCCRAC	4 cut(s)	1012, 2175, 2243, 2794
Mox20I	TGGCCA	1 cut(s)	1924
Mph1103I	ATGCAT	1 cut(s)	1252
MscI	TGGCCA	1 cut(s)	1924
MslI	CAYNNNNRTG	6 cut(s)	273, 461, 571, 1251, 2147, 2487
Msp20I	TGGCCA	1 cut(s)	1924
MspA1I	CMGCKG	2 cut(s)	1676, 1793
MspCI	CTTAAG	1 cut(s)	518
MspI	CCGG	1 cut(s)	1431
MspR9I	CCNGG	4 cut(s)	492, 723, 863, 1096
MunI	CAATTG	4 cut(s)	281, 331, 414, 834
Mva1269I	GAATGC	1 cut(s)	1236
MvaI	CCWGG	4 cut(s)	492, 723, 863, 1096
MwoI	GCNNNNNNNGC	4 cut(s)	956, 1295, 1403, 2589
NcoI	CCATGG	1 cut(s)	2323
NlaIV	GGNNCC	9 cut(s)	101, 367, 579, 1522, 1588, 1749, 2050, 2454, 2714
NmuCI	GTSAC	4 cut(s)	229, 498, 1684, 2381
NsiI	ATGCAT	1 cut(s)	1252
NspI	RCATGY	2 cut(s)	1250, 1254
PaeI	GCATGC	1 cut(s)	1250
PagI	TCATGA	2 cut(s)	141, 2227
PcsI	WCGNNNNNNNCGW	1 cut(s)	2540
PctI	GAATGC	1 cut(s)	1236
PfeI	GAWTC	6 cut(s)	256, 392, 1099, 1719, 1982, 2537
PflMI	CCANNNNNTGG	1 cut(s)	2027
PinAI	ACCGGT	1 cut(s)	1430
PkrI	GCNGC	7 cut(s)	173, 181, 199, 811, 1027, 1399, 1855
PleI	GAGTC	3 cut(s)	404, 1848, 2270
PpsI	GAGTC	3 cut(s)	404, 1848, 2270
Ppu21I	YACGTR	1 cut(s)	1148
PshBI	ATTAAT	1 cut(s)	1326
PsiI	TTATAA	1 cut(s)	2670
Psp124BI	GAGCTC	2 cut(s)	61, 141
Psp6I	CCWGG	4 cut(s)	490, 721, 861, 1094
PspFI	CCCAGC	1 cut(s)	175
PspGI	CCWGG	4 cut(s)	490, 721, 861, 1094
PspN4I	GGNNCC	9 cut(s)	101, 367, 579, 1522, 1588, 1749, 2050, 2454, 2714
PspPI	GGNCC	2 cut(s)	187, 2884
PstI	CTGCAG	3 cut(s)	691, 1901, 2635
PsuI	RGATCY	3 cut(s)	1893, 1911, 2048
PvuII	CAGCTG	1 cut(s)	1676
RsaI	GTAC	9 cut(s)	791, 901, 1146, 1486, 1522, 1789, 1829, 2481, 2726
RsaNI	GTAC	9 cut(s)	790, 900, 1145, 1485, 1521, 1788, 1828, 2480, 2725
RseI	CAYNNNNRTG	6 cut(s)	273, 461, 571, 1251, 2147, 2487
SacI	GAGCTC	2 cut(s)	61, 141
SatI	GCNGC	7 cut(s)	172, 180, 198, 810, 1026, 1398, 1854
Sau96I	GGNCC	2 cut(s)	187, 2884
ScaI	AGTACT	1 cut(s)	1829
SchI	GAGTC	3 cut(s)	404, 1849, 2270
ScrFI	CCNGG	4 cut(s)	492, 723, 863, 1096
SduI	GDGCHC	6 cut(s)	61, 141, 360, 952, 1424, 2351
SfcI	CTRYAG	4 cut(s)	481, 687, 1897, 2631
SinI	GGWCC	2 cut(s)	187, 2884
SmiMI	CAYNNNNRTG	6 cut(s)	273, 461, 571, 1251, 2147, 2487
SphI	GCATGC	1 cut(s)	1250
SsiI	CCGC	5 cut(s)	796, 1398, 1772, 1793, 1960
SstI	GAGCTC	2 cut(s)	61, 141
StyD4I	CCNGG	4 cut(s)	490, 721, 861, 1094
StyI	CCWWGG	2 cut(s)	243, 2323
TaaI	ACNGT	7 cut(s)	1555, 1653, 1952, 1975, 2188, 2396, 2724
TaiI	ACGT	3 cut(s)	456, 683, 1150
TaqI	TCGA	7 cut(s)	530, 921, 1197, 1722, 2525, 2543, 2619
TatI	WGTACW	5 cut(s)	899, 1484, 1787, 1827, 2479
TauI	GCSGC	1 cut(s)	1400
TfiI	GAWTC	6 cut(s)	256, 392, 1099, 1719, 1982, 2537
TscAI	CASTG	8 cut(s)	47, 234, 952, 1007, 1305, 1600, 1656, 2635
TseFI	GTSAC	4 cut(s)	229, 498, 1684, 2381
TseI	GCWGC	6 cut(s)	171, 179, 197, 809, 1025, 1853
Tsp45I	GTSAC	4 cut(s)	229, 498, 1684, 2381
TspGWI	ACGGA	4 cut(s)	200, 941, 2549, 2876
TspRI	CASTG	8 cut(s)	47, 234, 952, 1007, 1305, 1600, 1656, 2635
Van91I	CCANNNNNTGG	1 cut(s)	2027
Vha464I	CTTAAG	1 cut(s)	518
VneI	GTGCAC	1 cut(s)	948
VpaK11BI	GGWCC	2 cut(s)	187, 2884
VspI	ATTAAT	1 cut(s)	1326
XapI	RAATTY	5 cut(s)	70, 1168, 1533, 1636, 1669
XceI	RCATGY	2 cut(s)	1250, 1254
XmiI	GTMKAC	1 cut(s)	1186
ZrmI	AGTACT	1 cut(s)	1829
Zsp2I	ATGCAT	1 cut(s)	1252

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.