Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0003599

Physical Location & Seq

Genomic Coordinates Reverse (-)

33800969 .. 33803850

Gene Structure 2882 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G023260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2694 bp

ATGGAATACCAGTTTTCGTCTGGAGAGGGGAAGGATCGGGGGTCGTCTTCTGGAGCTGAGGTGGAGGGTTTGGCGCCACTGGATGGTGAAGTGAGGAACTCGATTTCGGAGGATATGTTTAACAACATTGCTGAGTTTATGAATTTCGATTCTTATGCTGGATGGTGCAGCAGCCCGGGAACAATGGAGCAGATTGTGGCGGCGTATCCATCGGTGAGTTATGCTCCTTTGGATGCCTTGAACTTCACACAACAGAACGGTGGAACATTGCCTGTGACTGAAGATGGTGGGACTTTTGATGAGGCGGGGAGTTCTTTTGATTGTGGTGACAAGATTGTGCTTCGGCAAACCCAATACAGGGCGTCAACTGATTCCCACCACACTCATGATGCAGCTGCTAAACCGAAGAATGGTTCTTTTCAACAGAATAATGTCATGGATATGGCAGACTGTGTGATCCCCAGGCCATATGGATGGTCACTTAATGAGAAGATGCTAAAGGCGTTGTCCTTTTTCAAGGAGTCTTTTGGTGGGGGAATTTTGGCACAAGTATGGGTTCCCATGAAGCATGCCTATTTGCTTGACCATGTTCTTACAGGGTATCGGGAAGTGTCGAGGATGTTTACATTTGCTGCAGAAGAAACGCAGGGTACTAGCCTGGGGCTTCCTGGCCGTGTATTTGTCTCCAAAGTTCCAGAGTGGGCATCAAATGTTAGCTATTACAACAAGGATGAGTACCTGAGGGTGGAACATGCAGCTAATCATCAGGTCCGTGGATCAATTGCTTTGCCGGTTTTTGATCTGAACTCTGAGAAGTCATGTTGTGGTGTGTTTGAACTTGTCAGTACAAAAGATAAGCTCAACTTTGATGCAGAGATGCAGATGGTTTGTGAGGCCCTCCAGGCTGTGAAGTTGAGGACCACTACGCCTCCCCGACTTCATCCCCAGTGCCTCTCAAAGAACCAAAGGGATGCTTTAACTGAAATTACTGATGTCTTACGAGCTGTGTGTCATGCACATACATTGCCGTTGGCTCTCACCTGGATTCCTTGTTGCTACTCTGAGGGTGATGCAGAGGAAATTAGAAGAGTACGTGTTCGAGGGGGTATTACAAAATCAAATGAAAAATGCATACTATGCATTGAGGAAACAGCTTGTTATATAAATGATAGAGCGATGCAAGGCTTTGTGCATGCATGTGCAGAACATCATCTTGAGGAAGGAAATGGTATTGCTGGGAAAGCACTTCAATCAAATCATCCCTTCTTCATTCATGATGTGAAGGTGTATGACATATATGACTATCCACTAGTTCATCATGCACGCAGGTATGGTTTGAACGCTGCTGTTGCAATCAGACTAAGGAGCACCTACACTGGTGATGATGACTATATCTTAGATTCTGAACAGCAACTTTTGTTAAACAACCTCTCGGGTACCATGCAGAGAATGTGTAAGAGTTTAAGGACAGTGTCAGATGCAGAATTGGCTGGGGTAGAAGGCTCCAACACTGGGTTTCAAAGGGAGGCAGTCTCGAATGCCCCACCAATCCCCAGGAGAGATTCTCAGACAACATCTTTAGATAGTGAAATGAAGTTAACTGAGAAGATGCCTCAAATAAGGCAGGTGGAGAAAAAGCGTAGTACAGCAGAGAAAAATGTGAGCTTGAGTGTTCTACAGCAATACTTCTCTGGGATCTGCCCCACTACACTAAAAAGGATCTGCAGACAACACGGGATCCCAAGATGGCCATCTCGTAAAATAAATAAGGTGAATCGTTCCTTGAAGAAAATACAAAGTGTACTTGATTCTGTTCAGGGGGTGGAAGGAGGATTGAAGTATGACCCAACCACTGGAGGGTTTGTGGCAACAAGGTCTACCATCCAAGAGTTTGATGCACAGAAAAATCTTTTCTTCTCTGAGAAGAACTTGCCTGCACAGAATATTGTTTCAAGTTTGGATATTATGTTGGAAGAGGATGGCTGTTGTATGAATGAACGTATGTTGGTTCCATCTGCTCATCAGGCCAAGGAAGAGGTAAGTAAACAACACATCCCTGTGGTTGATTGTAGTATGGATTCCAAGCCAATTGCAATTGATTTTGGATCATGCCAGCCGACTGACCATGACATCCTGCCTCATACTAGTCCGGAAAGTGCTATTGGGGTCTCATATTTGGTAAAAGAAGTCAACAAATGGGATCAAAGCAAGGGAAGTCCAAAATTAGAGAGTTATGACTGCCATGTTGTGTCTCAGAGCTCTTCTTCAAGCTTGACGCACTCATCAAATAGTTCTGGTTCAATGATGCAAGGTTGTTCGTCGAGTTCTCGGAGCTTTGATGAGCGAAACTACCCTAAAGTCAAAGCAAGAAATGATGAGAATGGATCGAAAATCATTGTGAAAGCTACTTATAAAGAAGATACAATTAGGTTCAAGTTTGAGCCTTCGGGTGGATGTCTTAAACTGTATGAAGAAGTTGCAAAGAGGTTGAGGTTGCAAGATGGAACTTTCCAGCTCAAGTATCTAGATGATGAACAAGAATGGGTGATGTTAGTCAGTGATGCAGATTTGCAGGAGTGCCTTGAGATATTGGATGACATTGGGACACGTAGTGTTAAGTTTATGGTTCGTGATATACCTTTTGATCTAAGCAGTTCCGGCAGTAGTAATTGTTTTCTGGCCCGGGGATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PB1 RWP-RK

Protein Analysis

897

Amino Acids

99.76

Weight (kDa)

5.66

Isoelectric Point (pI)

48.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GAF_NLP	PF22922	316 - 489	3.3e-63	NLP protein GAF domain
RWP-RK	PF02042	565 - 590	2.5e-11	RWP-RK domain
PB1	PF00564	799 - 877	1.8e-18	PB1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT2G43500 AT3G59580 AT3G59580 AT3G59580
fragaria_vesca	FvH4_1g24430 FvH4_1g24430 FvH4_1g24430
malus_domestica	MD04G1116200.v1.1 MD12G1131800.v1.1
prunus_persica	Prupe.1G200100_v2.0.a1
pyrus_communis	pycom04g10620 pycom12g12770
rosa_chinensis	RchiOBHm_Chr2g0121311
rosa_laevigata	RLG00000018617
rosa_multiflora	Rmu_sc0000079.1_g000028 Rmu_sc0000315.1_g000101
rosa_roxburghii	Rroxscaffold_2G00122460
rosa_rugosa	Rorug02G0233300 Rorug02G0233400 Rorug02G0233500
rosa_samantha	Rh2AG290100 Rh2BG301000 Rh2CG279700 Rh2DG314900
rosa_wichuraiana	Rw2G023260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2412
AarI	CACCTGC	1 cut(s)	1615
Acc36I	ACCTGC	2 cut(s)	1317, 1615
Acc65I	GGTACC	1 cut(s)	1436
AccB1I	GGYRCC	2 cut(s)	73, 1436
AccB7I	CCANNNNNTGG	2 cut(s)	83, 1853
AccI	GTMKAC	1 cut(s)	1877
AccIII	TCCGGA	1 cut(s)	2149
AciI	CCGC	2 cut(s)	200, 305
AcoI	YGGCCR	2 cut(s)	670, 1748
AcsI	RAATTY	2 cut(s)	142, 537
AcuI	CTGAAG	1 cut(s)	300
AcyI	GRCGYC	2 cut(s)	74, 362
AdeI	CACNNNGTG	1 cut(s)	2612
AfaI	GTAC	7 cut(s)	652, 737, 847, 1092, 1438, 1645, 1803
AfiI	CCNNNNNNNGG	9 cut(s)	83, 357, 358, 410, 745, 1512, 1853, 1857, 2450
AflIII	ACRYGT	2 cut(s)	1093, 2606
AhlI	ACTAGT	2 cut(s)	1309, 2144
AjnI	CCWGG	6 cut(s)	461, 657, 667, 900, 1040, 1553
AjuI	GAANNNNNNNTTGG	2 cut(s)	2145, 2177
Alw21I	GWGCWC	2 cut(s)	1370, 2261
Alw26I	GTCTC	4 cut(s)	688, 1537, 2173, 2256
Ama87I	CYCGRG	3 cut(s)	175, 1432, 2682
Aor13HI	TCCGGA	1 cut(s)	2149
AoxI	GGCC	6 cut(s)	464, 670, 894, 1748, 2025, 2679
ApeKI	GCWGC	7 cut(s)	168, 171, 392, 395, 632, 755, 1343
ApoI	RAATTY	2 cut(s)	142, 537
Asp718I	GGTACC	1 cut(s)	1436
AspLEI	GCGC	1 cut(s)	76
AspS9I	GGNCC	4 cut(s)	769, 895, 918, 2680
AsuC2I	CCSGG	4 cut(s)	176, 177, 2683, 2684
AsuHPI	GGTGA	8 cut(s)	98, 226, 338, 1030, 1079, 1391, 1783, 2557
AvaI	CYCGRG	3 cut(s)	175, 1432, 2682
AvaII	GGWCC	2 cut(s)	769, 918
AxyI	CCTNAGG	1 cut(s)	740
BalI	TGGCCA	1 cut(s)	1750
BamHI	GGATCC	1 cut(s)	1737
BanI	GGYRCC	2 cut(s)	73, 1436
BanII	GRGCYC	1 cut(s)	2261
BbsI	GAAGAC	1 cut(s)	39
Bbv12I	GWGCWC	2 cut(s)	1370, 2261
BbvCI	CCTCAGC	1 cut(s)	57
BbvI	GCAGC	7 cut(s)	180, 183, 382, 404, 619, 767, 1330
BceAI	ACGGC	2 cut(s)	657, 1012
BcgI	CGANNNNNNTGC	2 cut(s)	137, 171
BciT130I	CCWGG	6 cut(s)	463, 659, 669, 902, 1042, 1555
BciVI	GTATCC	1 cut(s)	216
BcnI	CCSGG	4 cut(s)	176, 177, 2683, 2684
BcoDI	GTCTC	4 cut(s)	688, 1537, 2173, 2256
BcuI	ACTAGT	2 cut(s)	1309, 2144
BfaI	CTAG	4 cut(s)	654, 1310, 2145, 2525
BfmI	CTRYAG	3 cut(s)	633, 1676, 1723
BfoI	RGCGCY	1 cut(s)	77
BfuAI	ACCTGC	2 cut(s)	1317, 1615
BfuI	GTATCC	1 cut(s)	216
BglI	GCCNNNNNGGC	1 cut(s)	902
BisI	GCNGC	8 cut(s)	169, 172, 201, 393, 396, 633, 756, 1344
BlsI	GCNGC	8 cut(s)	170, 173, 202, 394, 397, 634, 757, 1345
Bme18I	GGWCC	2 cut(s)	769, 918
BmeT110I	CYCGRG	3 cut(s)	175, 1432, 2682
BmgT120I	GGNCC	4 cut(s)	769, 895, 918, 2680
BmiI	GGNNCC	6 cut(s)	75, 558, 1438, 1504, 1739, 2010
BmrI	ACTGGG	2 cut(s)	940, 1521
BmuI	ACTGGG	2 cut(s)	940, 1521
BpiI	GAAGAC	1 cut(s)	39
BplI	GAGNNNNNCTC	6 cut(s)	208, 240, 1517, 1549, 1549, 1581
BpmI	CTGGAG	4 cut(s)	42, 72, 884, 1875
Bpu10I	CCTNAGC	1 cut(s)	57
BpuEI	CTTGAG	4 cut(s)	1235, 1687, 2501, 2603
BpuMI	CCSGG	4 cut(s)	176, 177, 2683, 2684
BsaAI	YACGTR	2 cut(s)	1094, 2609
BsaBI	GATNNNNATC	1 cut(s)	1750
BsaHI	GRCGYC	2 cut(s)	74, 362
BsaI	GGTCTC	1 cut(s)	2173
BsaJI	CCNNGG	8 cut(s)	175, 461, 658, 772, 1553, 2028, 2682, 2683
BsaWI	WCCGGW	1 cut(s)	2149
BsaXI	ACNNNNNCTCC	8 cut(s)	45, 75, 101, 131, 913, 943, 1848, 1878
Bsc4I	CCNNNNNNNGG	9 cut(s)	83, 357, 358, 410, 745, 1512, 1853, 1857, 2450
Bse118I	RCCGGY	1 cut(s)	790
Bse1I	ACTGG	6 cut(s)	10, 84, 946, 1381, 1516, 1858
Bse21I	CCTNAGG	1 cut(s)	740
Bse3DI	GCAATG	3 cut(s)	126, 266, 1022
Bse8I	GATNNNNATC	1 cut(s)	1750
BseAI	TCCGGA	1 cut(s)	2149
BseBI	CCWGG	6 cut(s)	463, 659, 669, 902, 1042, 1555
BseDI	CCNNGG	8 cut(s)	175, 461, 658, 772, 1553, 2028, 2682, 2683
BseJI	GATNNNNATC	1 cut(s)	1750
BseLI	CCNNNNNNNGG	9 cut(s)	83, 357, 358, 410, 745, 1512, 1853, 1857, 2450
BseMI	GCAATG	3 cut(s)	126, 266, 1022
BseMII	CTCAG	9 cut(s)	48, 123, 731, 801, 1053, 1580, 1593, 1911, 2267
BseNI	ACTGG	6 cut(s)	10, 84, 946, 1381, 1516, 1858
BseXI	GCAGC	7 cut(s)	180, 183, 382, 404, 619, 767, 1330
BseYI	CCCAGC	2 cut(s)	1235, 1490
BsgI	GTGCAG	3 cut(s)	187, 1221, 1920
BshFI	GGCC	6 cut(s)	466, 672, 896, 1750, 2027, 2681
BshNI	GGYRCC	2 cut(s)	73, 1436
BsiHKAI	GWGCWC	2 cut(s)	1370, 2261
BsiHKCI	CYCGRG	3 cut(s)	175, 1432, 2682
BsiSI	CCGG	5 cut(s)	176, 791, 2150, 2658, 2683
BslFI	GGGAC	2 cut(s)	304, 2617
BslI	CCNNNNNNNGG	9 cut(s)	83, 357, 358, 410, 745, 1512, 1853, 1857, 2450
BsmAI	GTCTC	4 cut(s)	688, 1537, 2173, 2256
BsmFI	GGGAC	2 cut(s)	304, 2617
BsmI	GAATGC	1 cut(s)	1543
BsnI	GGCC	6 cut(s)	466, 672, 896, 1750, 2027, 2681
Bso31I	GGTCTC	1 cut(s)	2173
BsoBI	CYCGRG	3 cut(s)	175, 1432, 2682
Bsp1286I	GDGCHC	2 cut(s)	1370, 2261
Bsp13I	TCCGGA	1 cut(s)	2149
BspACI	CCGC	2 cut(s)	200, 305
BspANI	GGCC	6 cut(s)	466, 672, 896, 1750, 2027, 2681
BspCNI	CTCAG	9 cut(s)	49, 124, 732, 802, 1054, 1579, 1594, 1912, 2266
BspEI	TCCGGA	1 cut(s)	2149
BspHI	TCATGA	2 cut(s)	385, 1273
BspLI	GGNNCC	6 cut(s)	75, 558, 1438, 1504, 1739, 2010
BspMAI	CTGCAG	2 cut(s)	637, 1727
BspMI	ACCTGC	2 cut(s)	1317, 1615
BspQI	GCTCTTC	1 cut(s)	2266
BspT107I	GGYRCC	2 cut(s)	73, 1436
BspTNI	GGTCTC	1 cut(s)	2173
BsrDI	GCAATG	3 cut(s)	126, 266, 1022
BsrFI	RCCGGY	1 cut(s)	790
BsrI	ACTGG	6 cut(s)	10, 84, 946, 1381, 1516, 1858
BssAI	RCCGGY	1 cut(s)	790
BssECI	CCNNGG	8 cut(s)	175, 461, 658, 772, 1553, 2028, 2682, 2683
BssNI	GRCGYC	2 cut(s)	74, 362
BssT1I	CCWWGG	1 cut(s)	2028
Bst2UI	CCWGG	6 cut(s)	463, 659, 669, 902, 1042, 1555
Bst4CI	ACNGT	4 cut(s)	260, 452, 1471, 2466
Bst6I	CTCTTC	4 cut(s)	1081, 1968, 2028, 2266
BstACI	GRCGYC	2 cut(s)	74, 362
BstAPI	GCANNNNNTGC	1 cut(s)	1137
BstBAI	YACGTR	2 cut(s)	1094, 2609
BstC8I	GCNNGC	5 cut(s)	570, 1194, 1324, 1935, 2114
BstDSI	CCRYGG	1 cut(s)	772
BstH2I	RGCGCY	1 cut(s)	77
BstHHI	GCGC	1 cut(s)	76
BstMAI	GTCTC	4 cut(s)	688, 1537, 2173, 2256
BstMWI	GCNNNNNNNGC	7 cut(s)	902, 1137, 1241, 1349, 1487, 2024, 2658
BstNI	CCWGG	6 cut(s)	463, 659, 669, 902, 1042, 1555
BstNSI	RCATGY	4 cut(s)	572, 755, 1196, 1200
BstSFI	CTRYAG	3 cut(s)	633, 1676, 1723
BstV1I	GCAGC	7 cut(s)	180, 183, 382, 404, 619, 767, 1330
BstV2I	GAAGAC	1 cut(s)	39
BstX2I	RGATCY	4 cut(s)	1695, 1719, 1737, 2687
BstXI	CCANNNNNNTGG	1 cut(s)	474
BstYI	RGATCY	4 cut(s)	1695, 1719, 1737, 2687
Bsu36I	CCTNAGG	1 cut(s)	740
BsuI	GTATCC	1 cut(s)	216
BsuRI	GGCC	6 cut(s)	466, 672, 896, 1750, 2027, 2681
BtgI	CCRYGG	1 cut(s)	772
BtgZI	GCGATG	1 cut(s)	1190
BtsIMutI	CAGTG	7 cut(s)	77, 953, 1374, 1476, 1509, 1851, 2563
BveI	ACCTGC	2 cut(s)	1317, 1615
Cac8I	GCNNGC	5 cut(s)	570, 1194, 1324, 1935, 2114
CciI	TCATGA	2 cut(s)	385, 1273
CfoI	GCGC	1 cut(s)	76
Cfr10I	RCCGGY	1 cut(s)	790
Cfr13I	GGNCC	4 cut(s)	769, 895, 918, 2680
Cfr9I	CCCGGG	2 cut(s)	175, 2682
CseI	GACGC	2 cut(s)	351, 2284
Csp6I	GTAC	7 cut(s)	651, 736, 846, 1091, 1437, 1644, 1802
CviQI	GTAC	7 cut(s)	651, 736, 846, 1091, 1437, 1644, 1802
DinI	GGCGCC	1 cut(s)	75
DraIII	CACNNNGTG	1 cut(s)	2612
EaeI	YGGCCR	2 cut(s)	670, 1748
Eam1104I	CTCTTC	4 cut(s)	1081, 1968, 2028, 2266
EarI	CTCTTC	4 cut(s)	1081, 1968, 2028, 2266
Ecl136II	GAGCTC	1 cut(s)	2259
Eco130I	CCWWGG	1 cut(s)	2028
Eco24I	GRGCYC	1 cut(s)	2261
Eco31I	GGTCTC	1 cut(s)	2173
Eco47I	GGWCC	2 cut(s)	769, 918
Eco53kI	GAGCTC	1 cut(s)	2259
Eco57I	CTGAAG	1 cut(s)	300
Eco81I	CCTNAGG	1 cut(s)	740
Eco88I	CYCGRG	3 cut(s)	175, 1432, 2682
EcoICRI	GAGCTC	1 cut(s)	2259
EcoO109I	RGGNCCY	1 cut(s)	895
EcoRII	CCWGG	6 cut(s)	461, 657, 667, 900, 1040, 1553
EcoT14I	CCWWGG	1 cut(s)	2028
EcoT22I	ATGCAT	3 cut(s)	1133, 1142, 1198
EcoT38I	GRGCYC	1 cut(s)	2261
EgeI	GGCGCC	1 cut(s)	75
EheI	GGCGCC	1 cut(s)	75
ErhI	CCWWGG	1 cut(s)	2028
FalI	AAGNNNNNCTT	4 cut(s)	848, 880, 958, 990
FaqI	GGGAC	2 cut(s)	304, 2617
FauI	CCCGC	1 cut(s)	298
FauNDI	CATATG	1 cut(s)	469
FblI	GTMKAC	1 cut(s)	1877
Fnu4HI	GCNGC	8 cut(s)	169, 172, 201, 393, 396, 633, 756, 1344
FriOI	GRGCYC	1 cut(s)	2261
Fsp4HI	GCNGC	8 cut(s)	169, 172, 201, 393, 396, 633, 756, 1344
FspBI	CTAG	4 cut(s)	654, 1310, 2145, 2525
GlaI	GCGC	1 cut(s)	75
GluI	GCNGC	8 cut(s)	169, 172, 201, 393, 396, 633, 756, 1344
GsaI	CCCAGC	2 cut(s)	1239, 1494
GsuI	CTGGAG	4 cut(s)	42, 72, 884, 1875
HaeII	RGCGCY	1 cut(s)	77
HaeIII	GGCC	6 cut(s)	466, 672, 896, 1750, 2027, 2681
HapII	CCGG	5 cut(s)	176, 791, 2150, 2658, 2683
HgaI	GACGC	2 cut(s)	351, 2284
HhaI	GCGC	1 cut(s)	76
Hin1I	GRCGYC	2 cut(s)	74, 362
Hin6I	GCGC	1 cut(s)	74
HinP1I	GCGC	1 cut(s)	74
HincII	GTYRAC	3 cut(s)	366, 1599, 2191
HindII	GTYRAC	3 cut(s)	366, 1599, 2191
HindIII	AAGCTT	1 cut(s)	2269
HinfI	GANTC	9 cut(s)	149, 371, 521, 1045, 1400, 1562, 1774, 1808, 2078
HpaI	GTTAAC	1 cut(s)	1599
HpaII	CCGG	5 cut(s)	176, 791, 2150, 2658, 2683
HphI	GGTGA	8 cut(s)	98, 226, 338, 1030, 1079, 1391, 1783, 2557
Hpy166II	GTNNAC	7 cut(s)	366, 624, 1599, 1802, 1878, 2045, 2191
Hpy8I	GTNNAC	7 cut(s)	366, 624, 1599, 1802, 1878, 2045, 2191
Hpy99I	CGWCG	1 cut(s)	2323
HpyAV	CCTTC	7 cut(s)	25, 1214, 1273, 1276, 1493, 1820, 2454
HpyCH4III	ACNGT	4 cut(s)	260, 452, 1471, 2466
HpyCH4IV	ACGT	3 cut(s)	1093, 1999, 2608
HpyF10VI	GCNNNNNNNGC	7 cut(s)	902, 1137, 1241, 1349, 1487, 2024, 2658
HpySE526I	ACGT	3 cut(s)	1093, 1999, 2608
Hsp92I	GRCGYC	2 cut(s)	74, 362
HspAI	GCGC	1 cut(s)	74
KasI	GGCGCC	1 cut(s)	73
Kpn2I	TCCGGA	1 cut(s)	2149
KpnI	GGTACC	1 cut(s)	1440
KspAI	GTTAAC	1 cut(s)	1599
LguI	GCTCTTC	1 cut(s)	2266
LmnI	GCTCC	6 cut(s)	53, 187, 229, 1365, 1508, 2331
Lsp1109I	GCAGC	7 cut(s)	180, 183, 382, 404, 619, 767, 1330
MaeI	CTAG	4 cut(s)	654, 1310, 2145, 2525
MaeII	ACGT	3 cut(s)	1093, 1999, 2608
MaeIII	GTNAC	3 cut(s)	274, 326, 477
MfeI	CAATTG	3 cut(s)	780, 2088, 2094
MflI	RGATCY	4 cut(s)	1695, 1719, 1737, 2687
MhlI	GDGCHC	2 cut(s)	1370, 2261
MlsI	TGGCCA	1 cut(s)	1750
MluNI	TGGCCA	1 cut(s)	1750
Mly113I	GGCGCC	1 cut(s)	74
MlyI	GAGTC	1 cut(s)	530
MmeI	TCCRAC	2 cut(s)	1530, 1950
Mox20I	TGGCCA	1 cut(s)	1750
Mph1103I	ATGCAT	3 cut(s)	1133, 1142, 1198
MroI	TCCGGA	1 cut(s)	2149
MscI	TGGCCA	1 cut(s)	1750
MseI	TTAA	8 cut(s)	120, 483, 977, 1421, 1463, 1598, 2460, 2616
MslI	CAYNNNNRTG	6 cut(s)	384, 440, 472, 550, 1197, 2057
Msp20I	TGGCCA	1 cut(s)	1750
MspA1I	CMGCKG	1 cut(s)	395
MspI	CCGG	5 cut(s)	176, 791, 2150, 2658, 2683
MunI	CAATTG	3 cut(s)	780, 2088, 2094
Mva1269I	GAATGC	1 cut(s)	1543
MvaI	CCWGG	6 cut(s)	463, 659, 669, 902, 1042, 1555
MwoI	GCNNNNNNNGC	7 cut(s)	902, 1137, 1241, 1349, 1487, 2024, 2658
NarI	GGCGCC	1 cut(s)	74
NciI	CCSGG	4 cut(s)	176, 177, 2683, 2684
NdeI	CATATG	1 cut(s)	469
NlaIV	GGNNCC	6 cut(s)	75, 558, 1438, 1504, 1739, 2010
NmuCI	GTSAC	3 cut(s)	274, 326, 477
NsiI	ATGCAT	3 cut(s)	1133, 1142, 1198
NspI	RCATGY	4 cut(s)	572, 755, 1196, 1200
PaeI	GCATGC	2 cut(s)	572, 1196
PagI	TCATGA	2 cut(s)	385, 1273
PaqCI	CACCTGC	1 cut(s)	1615
PciSI	GCTCTTC	1 cut(s)	2266
PctI	GAATGC	1 cut(s)	1543
PfeI	GAWTC	8 cut(s)	149, 371, 1045, 1400, 1562, 1774, 1808, 2078
PflFI	GACNNNGTC	1 cut(s)	1471
PflMI	CCANNNNNTGG	2 cut(s)	83, 1853
PkrI	GCNGC	8 cut(s)	170, 173, 202, 394, 397, 634, 757, 1345
PleI	GAGTC	1 cut(s)	529
PluTI	GGCGCC	1 cut(s)	77
PpsI	GAGTC	1 cut(s)	529
Ppu21I	YACGTR	2 cut(s)	1094, 2609
PsiI	TTATAA	1 cut(s)	2412
Psp124BI	GAGCTC	1 cut(s)	2261
Psp6I	CCWGG	6 cut(s)	461, 657, 667, 900, 1040, 1553
PspFI	CCCAGC	2 cut(s)	1235, 1490
PspGI	CCWGG	6 cut(s)	461, 657, 667, 900, 1040, 1553
PspN4I	GGNNCC	6 cut(s)	75, 558, 1438, 1504, 1739, 2010
PspPI	GGNCC	4 cut(s)	769, 895, 918, 2680
PstI	CTGCAG	2 cut(s)	637, 1727
PsuI	RGATCY	4 cut(s)	1695, 1719, 1737, 2687
PsyI	GACNNNGTC	1 cut(s)	1471
PvuII	CAGCTG	1 cut(s)	395
RsaI	GTAC	7 cut(s)	652, 737, 847, 1092, 1438, 1645, 1803
RsaNI	GTAC	7 cut(s)	651, 736, 846, 1091, 1437, 1644, 1802
RseI	CAYNNNNRTG	6 cut(s)	384, 440, 472, 550, 1197, 2057
SacI	GAGCTC	1 cut(s)	2261
SapI	GCTCTTC	1 cut(s)	2266
SaqAI	TTAA	8 cut(s)	120, 483, 977, 1421, 1463, 1598, 2460, 2616
SatI	GCNGC	8 cut(s)	169, 172, 201, 393, 396, 633, 756, 1344
Sau96I	GGNCC	4 cut(s)	769, 895, 918, 2680
SchI	GAGTC	1 cut(s)	530
SduI	GDGCHC	2 cut(s)	1370, 2261
SfcI	CTRYAG	3 cut(s)	633, 1676, 1723
SfoI	GGCGCC	1 cut(s)	75
SinI	GGWCC	2 cut(s)	769, 918
SmaI	CCCGGG	2 cut(s)	177, 2684
SmiMI	CAYNNNNRTG	6 cut(s)	384, 440, 472, 550, 1197, 2057
SmlI	CTYRAG	4 cut(s)	1214, 1666, 2516, 2582
SmoI	CTYRAG	4 cut(s)	1214, 1666, 2516, 2582
SpeI	ACTAGT	2 cut(s)	1309, 2144
SphI	GCATGC	2 cut(s)	572, 1196
SsiI	CCGC	2 cut(s)	200, 305
SspDI	GGCGCC	1 cut(s)	73
SspI	AATATT	1 cut(s)	1945
SspMI	CTAG	4 cut(s)	654, 1310, 2145, 2525
SstI	GAGCTC	1 cut(s)	2261
StyI	CCWWGG	1 cut(s)	2028
TaaI	ACNGT	4 cut(s)	260, 452, 1471, 2466
TaiI	ACGT	3 cut(s)	1096, 2002, 2611
TaqI	TCGA	7 cut(s)	101, 147, 614, 1099, 1535, 2321, 2387
TatI	WGTACW	3 cut(s)	845, 1643, 1801
TauI	GCSGC	1 cut(s)	203
TfiI	GAWTC	8 cut(s)	149, 371, 1045, 1400, 1562, 1774, 1808, 2078
Tru1I	TTAA	8 cut(s)	120, 483, 977, 1421, 1463, 1598, 2460, 2616
Tru9I	TTAA	8 cut(s)	120, 483, 977, 1421, 1463, 1598, 2460, 2616
TscAI	CASTG	7 cut(s)	84, 953, 1381, 1476, 1516, 1858, 2563
TseFI	GTSAC	3 cut(s)	274, 326, 477
TseI	GCWGC	7 cut(s)	168, 171, 392, 395, 632, 755, 1343
Tsp45I	GTSAC	3 cut(s)	274, 326, 477
TspGWI	ACGGA	1 cut(s)	761
TspMI	CCCGGG	2 cut(s)	175, 2682
TspRI	CASTG	7 cut(s)	84, 953, 1381, 1476, 1516, 1858, 2563
Tth111I	GACNNNGTC	1 cut(s)	1471
Van91I	CCANNNNNTGG	2 cut(s)	83, 1853
VpaK11BI	GGWCC	2 cut(s)	769, 918
XapI	RAATTY	2 cut(s)	142, 537
XbaI	TCTAGA	1 cut(s)	2524
XceI	RCATGY	4 cut(s)	572, 755, 1196, 1200
XcmI	CCANNNNNNNNNTGG	1 cut(s)	17
XmaI	CCCGGG	2 cut(s)	175, 2682
XmiI	GTMKAC	1 cut(s)	1877
XspI	CTAG	4 cut(s)	654, 1310, 2145, 2525
Zsp2I	ATGCAT	3 cut(s)	1133, 1142, 1198

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.