MD04G1123300.v1.1

UNC93-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006088
Physical Location & Seq
Forward (+)
20893255 .. 20896400
3146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1123300.v1.1.491

Sequence Viewer

Length: 1338 bp
ATGGAGTCTGCTGTTATGGTCTCTGCAGCTTCTTTTCATGATCTGGAGTCTTCTCATGAGCCGGAGAAGCCACTCCTTGTCGATAATTCTCGAACCCGAAAGAACCGTGCCCGAGATGTTCATATTCTCAGCTCTGCCTTCTTGCTCATCTTTCTTGCTTTTGGTGCCACTCAGAATCTAGAGAGCACTCTCATCACTGAGAAGAATTTGGGTACCACATCACTTGGAATTTTGTATCTATCATTCGCATTTTTCTCTTTGGTGGCGTCCTCGGTTGTTCGACGTTTGGGTTCAAAGAACTCTTTGATTCTTGGGACTACTGGCTATTGGTTGTTCATAGCTGCACATTTGAAACCAACTTGGTACATAATGGTTCCGGTTTCTTTGTACCTTGGGTTTGCCTCTTCAATAATATGGGTCGAGCAGGGAGCATATCTAACTGCAGCAGCGCGTAGTCATGCCCGAGACAATGGATTACATGAAGCAACAGTTATTGGCAACTTCAATGGAGAATTTTGGGGAATGTTTGCCTGTCGACAGTTCGTTGGAAATCTTTTACCACTCTTCTTGCTAAAAAGTGGAACAGACGGAAATGCAGGCGACATAACTTTGTTGTTCACTGTGTTTCTCTGCAGTATGACCTTAGGAACCATACTGATGTGCTTCTTAAGCAAACGGGATGAAGTAGAGCACCCCCACGGTTCTTCTCTCAACTTACTTGCTACTCTGTCATCTTCGTCAATGTCGCTCATCCCTCCTTTGTTTGATATACGGATGCTATTGATCATCCCCCTTATGGCGTATACAGGATTACAACAAGCATTTGTATGGGCTGAATTCACCAAGTTCATCGTTAAACCAATGTTCGGTGTGTCTGGTGTGGGCGGTGCAATGGCAGTTTATGGGGCTTTCGATGCAATATGTTCACTCACTGCTGGTCGACTTACCTCGGGTCTCAAGTCCATCACTCTTATCGTCACAGGCGGAGCTTTAGTTCAGGCTGTCGTATTCATCTGGCTGCTTCTATTTTACAGCCCGTCAAGCGGACTACATGGCACTATGAATCTTCTTATAATGGCAGCTCTGTTGGGCATCGGGGATGGGGTTTTCAACACACAGCTCAGTGCTTTGATTGGAATATTCTTCAAACACGATACGGAAGGAGCATTTGCACAGCTCAAGGTGTGGCAGTGTGGCGGCGTTGCACTCATCTTCTTCATCAGTCCATACATCTCATTACAGGCAATGCTGGTGCTTATGCTCTCTGCAATCTGCCTCGCATACGCCTCCTTTCTATTTCTGCATCGTCGAGGAGACAAATCAATCTCGCTTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UNC-93

Protein Analysis

446

Amino Acids

48.33

Weight (kDa)

8.48

Isoelectric Point (pI)

25.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UNC-93 PF05978 67 - 431 2.1e-35 Ion channel regulatory protein UNC-93
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1073
Acc65I GGTACC 1 cut(s) 212
AccB1I GGYRCC 2 cut(s) 164, 212
AccI GTMKAC 3 cut(s) 535, 803, 940
AccII CGCG 1 cut(s) 451
AciI CCGC 4 cut(s) 885, 984, 1044, 1197
AcsI RAATTY 4 cut(s) 205, 228, 512, 836
AcyI GRCGYC 1 cut(s) 266
AfaI GTAC 3 cut(s) 214, 365, 389
AfiI CCNNNNNNNGG 3 cut(s) 796, 866, 1043
AflII CTTAAG 1 cut(s) 667
AgsI TTSAA 6 cut(s) 294, 352, 408, 505, 1111, 1147
AloI GAACNNNNNNTCC 2 cut(s) 978, 1010
AluBI AGCT 7 cut(s) 29, 132, 341, 989, 1082, 1120, 1177
AluI AGCT 7 cut(s) 29, 132, 341, 989, 1082, 1120, 1177
Alw21I GWGCWC 2 cut(s) 188, 693
Alw26I GTCTC 4 cut(s) 25, 459, 959, 1308
Ama87I CYCGRG 3 cut(s) 111, 462, 949
ApeKI GCWGC 6 cut(s) 26, 341, 443, 446, 1018, 1079
ApoI RAATTY 4 cut(s) 205, 228, 512, 836
Asp718I GGTACC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 451
AsuHPI GGTGA 1 cut(s) 832
AvaI CYCGRG 3 cut(s) 111, 462, 949
AxyI CCTNAGG 1 cut(s) 643
BaeGI GKGCMC 1 cut(s) 112
BanI GGYRCC 2 cut(s) 164, 212
BbsI GAAGAC 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 188, 693
BbvI GCAGC 6 cut(s) 38, 328, 455, 458, 1005, 1091
BccI CCATC 2 cut(s) 971, 1094
BclI TGATCA 1 cut(s) 783
BcoDI GTCTC 4 cut(s) 25, 459, 959, 1308
BfaI CTAG 1 cut(s) 179
BfmI CTRYAG 3 cut(s) 24, 441, 631
BfrI CTTAAG 1 cut(s) 667
BisI GCNGC 7 cut(s) 27, 342, 444, 447, 1019, 1080, 1198
BlsI GCNGC 7 cut(s) 28, 343, 445, 448, 1020, 1081, 1199
BmeT110I CYCGRG 3 cut(s) 111, 462, 949
BmiI GGNNCC 4 cut(s) 166, 214, 375, 649
BmsI GCATC 4 cut(s) 765, 904, 1101, 1312
BpiI GAAGAC 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 65
BpuEI CTTGAG 2 cut(s) 941, 1163
BsaHI GRCGYC 1 cut(s) 266
BsaI GGTCTC 2 cut(s) 25, 959
BsaJI CCNNGG 4 cut(s) 270, 391, 697, 948
BsaWI WCCGGW 1 cut(s) 376
BsaXI ACNNNNNCTCC 2 cut(s) 978, 1008
Bsc4I CCNNNNNNNGG 3 cut(s) 796, 866, 1043
Bse1I ACTGG 1 cut(s) 325
Bse21I CCTNAGG 1 cut(s) 643
Bse3DI GCAATG 2 cut(s) 897, 1251
BseDI CCNNGG 4 cut(s) 270, 391, 697, 948
BseGI GGATG 5 cut(s) 685, 750, 780, 786, 1105
BseLI CCNNNNNNNGG 3 cut(s) 796, 866, 1043
BseMI GCAATG 2 cut(s) 897, 1251
BseMII CTCAG 4 cut(s) 142, 185, 189, 1135
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 1 cut(s) 1326
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 6 cut(s) 38, 328, 455, 458, 1005, 1091
BsgI GTGCAG 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 451
BshNI GGYRCC 2 cut(s) 164, 212
BsiHKAI GWGCWC 2 cut(s) 188, 693
BsiHKCI CYCGRG 3 cut(s) 111, 462, 949
BsiSI CCGG 2 cut(s) 62, 377
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 3 cut(s) 796, 866, 1043
BsmAI GTCTC 4 cut(s) 25, 459, 959, 1308
BsmFI GGGAC 1 cut(s) 328
Bso31I GGTCTC 2 cut(s) 25, 959
BsoBI CYCGRG 3 cut(s) 111, 462, 949
Bsp1286I GDGCHC 3 cut(s) 112, 188, 693
Bsp143I GATC 2 cut(s) 40, 783
BspACI CCGC 4 cut(s) 885, 984, 1044, 1197
BspCNI CTCAG 4 cut(s) 141, 184, 190, 1134
BspFNI CGCG 1 cut(s) 451
BspHI TCATGA 2 cut(s) 37, 55
BspLI GGNNCC 4 cut(s) 166, 214, 375, 649
BspMAI CTGCAG 3 cut(s) 28, 445, 635
BspT107I GGYRCC 2 cut(s) 164, 212
BspTI CTTAAG 1 cut(s) 667
BspTNI GGTCTC 2 cut(s) 25, 959
BsrDI GCAATG 2 cut(s) 897, 1251
BsrI ACTGG 1 cut(s) 325
BssECI CCNNGG 4 cut(s) 270, 391, 697, 948
BssMI GATC 2 cut(s) 40, 783
BssNAI GTATAC 1 cut(s) 804
BssNI GRCGYC 1 cut(s) 266
BssT1I CCWWGG 1 cut(s) 391
Bst1107I GTATAC 1 cut(s) 804
Bst4CI ACNGT 5 cut(s) 107, 490, 540, 622, 701
Bst6I CTCTTC 2 cut(s) 409, 569
BstACI GRCGYC 1 cut(s) 266
BstAFI CTTAAG 1 cut(s) 667
BstC8I GCNNGC 1 cut(s) 598
BstDEI CTNAG 5 cut(s) 128, 171, 198, 643, 1121
BstDSI CCRYGG 1 cut(s) 697
BstF5I GGATG 5 cut(s) 685, 750, 780, 786, 1105
BstFNI CGCG 1 cut(s) 451
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 2 cut(s) 43, 786
BstMAI GTCTC 4 cut(s) 25, 459, 959, 1308
BstMBI GATC 2 cut(s) 40, 783
BstMWI GCNNNNNNNGC 5 cut(s) 67, 164, 669, 914, 1041
BstSFI CTRYAG 3 cut(s) 24, 441, 631
BstSLI GKGCMC 1 cut(s) 112
BstUI CGCG 1 cut(s) 451
BstV1I GCAGC 6 cut(s) 38, 328, 455, 458, 1005, 1091
BstV2I GAAGAC 1 cut(s) 42
BstZ17I GTATAC 1 cut(s) 804
Bsu36I CCTNAGG 1 cut(s) 643
BtgI CCRYGG 1 cut(s) 697
BtsCI GGATG 5 cut(s) 685, 750, 780, 786, 1105
BtsI GCAGTG 2 cut(s) 930, 1196
BtsIMutI CAGTG 5 cut(s) 195, 618, 930, 1129, 1196
Cac8I GCNNGC 1 cut(s) 598
CciI TCATGA 2 cut(s) 37, 55
CfoI GCGC 1 cut(s) 451
CseI GACGC 1 cut(s) 255
Csp6I GTAC 3 cut(s) 213, 364, 388
CspCI CAANNNNNGTGG 4 cut(s) 205, 240, 549, 584
CviAII CATG 5 cut(s) 38, 56, 458, 479, 1052
CviQI GTAC 3 cut(s) 213, 364, 388
DdeI CTNAG 5 cut(s) 128, 171, 198, 643, 1121
DpnI GATC 2 cut(s) 42, 785
DpnII GATC 2 cut(s) 40, 783
Eam1104I CTCTTC 2 cut(s) 409, 569
EarI CTCTTC 2 cut(s) 409, 569
EciI GGCGGA 1 cut(s) 999
Eco130I CCWWGG 1 cut(s) 391
Eco31I GGTCTC 2 cut(s) 25, 959
Eco81I CCTNAGG 1 cut(s) 643
Eco88I CYCGRG 3 cut(s) 111, 462, 949
EcoRI GAATTC 1 cut(s) 836
EcoT14I CCWWGG 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 5 cut(s) 41, 59, 461, 482, 1055
FaqI GGGAC 1 cut(s) 328
FatI CATG 5 cut(s) 37, 55, 457, 478, 1051
FbaI TGATCA 1 cut(s) 783
FblI GTMKAC 3 cut(s) 535, 803, 940
Fnu4HI GCNGC 7 cut(s) 27, 342, 444, 447, 1019, 1080, 1198
FokI GGATG 5 cut(s) 692, 737, 773, 787, 1112
Fsp4HI GCNGC 7 cut(s) 27, 342, 444, 447, 1019, 1080, 1198
FspBI CTAG 1 cut(s) 179
GlaI GCGC 1 cut(s) 450
GluI GCNGC 7 cut(s) 27, 342, 444, 447, 1019, 1080, 1198
GsuI CTGGAG 1 cut(s) 65
HapII CCGG 2 cut(s) 62, 377
HgaI GACGC 1 cut(s) 255
HhaI GCGC 1 cut(s) 451
Hin1I GRCGYC 1 cut(s) 266
Hin1II CATG 5 cut(s) 41, 59, 461, 482, 1055
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HincII GTYRAC 2 cut(s) 536, 941
HindII GTYRAC 2 cut(s) 536, 941
HinfI GANTC 5 cut(s) 5, 47, 175, 307, 1063
HpaII CCGG 2 cut(s) 62, 377
HphI GGTGA 1 cut(s) 832
Hpy166II GTNNAC 5 cut(s) 536, 618, 804, 926, 941
Hpy188I TCNGA 1 cut(s) 174
Hpy188III TCNNGA 5 cut(s) 38, 44, 56, 90, 179
Hpy8I GTNNAC 5 cut(s) 536, 618, 804, 926, 941
Hpy99I CGWCG 2 cut(s) 285, 1311
HpyAV CCTTC 2 cut(s) 148, 1154
HpyCH4III ACNGT 5 cut(s) 107, 490, 540, 622, 701
HpyCH4IV ACGT 1 cut(s) 283
HpyF10VI GCNNNNNNNGC 5 cut(s) 67, 164, 669, 914, 1041
HpyF3I CTNAG 5 cut(s) 128, 171, 198, 643, 1121
HpySE526I ACGT 1 cut(s) 283
Hsp92I GRCGYC 1 cut(s) 266
Hsp92II CATG 5 cut(s) 41, 59, 461, 482, 1055
HspAI GCGC 1 cut(s) 449
KpnI GGTACC 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 783
Kzo9I GATC 2 cut(s) 40, 783
LmnI GCTCC 3 cut(s) 428, 986, 1163
Lsp1109I GCAGC 6 cut(s) 38, 328, 455, 458, 1005, 1091
LweI GCATC 4 cut(s) 765, 904, 1101, 1312
MaeI CTAG 1 cut(s) 179
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 1 cut(s) 976
MalI GATC 2 cut(s) 42, 785
MboI GATC 2 cut(s) 40, 783
MhlI GDGCHC 3 cut(s) 112, 188, 693
MluCI AATT 5 cut(s) 85, 205, 228, 512, 836
MlyI GAGTC 2 cut(s) 14, 56
MmeI TCCRAC 1 cut(s) 526
MnlI CCTC 7 cut(s) 280, 412, 765, 958, 1286, 1297, 1304
MseI TTAA 3 cut(s) 668, 855, 1336
MslI CAYNNNNRTG 2 cut(s) 656, 826
MspCI CTTAAG 1 cut(s) 667
MspI CCGG 2 cut(s) 62, 377
MvnI CGCG 1 cut(s) 451
MwoI GCNNNNNNNGC 5 cut(s) 67, 164, 669, 914, 1041
NdeII GATC 2 cut(s) 40, 783
NlaIII CATG 5 cut(s) 41, 59, 461, 482, 1055
NlaIV GGNNCC 4 cut(s) 166, 214, 375, 649
NmuCI GTSAC 1 cut(s) 976
PagI TCATGA 2 cut(s) 37, 55
PfeI GAWTC 3 cut(s) 175, 307, 1063
PkrI GCNGC 7 cut(s) 28, 343, 445, 448, 1020, 1081, 1199
PleI GAGTC 2 cut(s) 13, 55
PpsI GAGTC 2 cut(s) 13, 55
PsiI TTATAA 1 cut(s) 1073
PspN4I GGNNCC 4 cut(s) 166, 214, 375, 649
PstI CTGCAG 3 cut(s) 28, 445, 635
RsaI GTAC 3 cut(s) 214, 365, 389
RsaNI GTAC 3 cut(s) 213, 364, 388
RseI CAYNNNNRTG 2 cut(s) 656, 826
SalI GTCGAC 2 cut(s) 534, 939
SaqAI TTAA 3 cut(s) 668, 855, 1336
SatI GCNGC 7 cut(s) 27, 342, 444, 447, 1019, 1080, 1198
Sau3AI GATC 2 cut(s) 40, 783
SchI GAGTC 2 cut(s) 14, 56
SduI GDGCHC 3 cut(s) 112, 188, 693
SfaNI GCATC 4 cut(s) 765, 904, 1101, 1312
SfcI CTRYAG 3 cut(s) 24, 441, 631
SmiMI CAYNNNNRTG 2 cut(s) 656, 826
SmlI CTYRAG 3 cut(s) 667, 956, 1178
SmoI CTYRAG 3 cut(s) 667, 956, 1178
Sse9I AATT 5 cut(s) 85, 205, 228, 512, 836
SsiI CCGC 4 cut(s) 885, 984, 1044, 1197
SspI AATATT 1 cut(s) 1140
SspMI CTAG 1 cut(s) 179
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 5 cut(s) 107, 490, 540, 622, 701
TaiI ACGT 1 cut(s) 286
TaqI TCGA 8 cut(s) 81, 91, 280, 420, 535, 912, 940, 1309
TasI AATT 5 cut(s) 85, 205, 228, 512, 836
TauI GCSGC 1 cut(s) 1200
TfiI GAWTC 3 cut(s) 175, 307, 1063
Tru1I TTAA 3 cut(s) 668, 855, 1336
Tru9I TTAA 3 cut(s) 668, 855, 1336
TscAI CASTG 5 cut(s) 202, 625, 937, 1129, 1196
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 6 cut(s) 26, 341, 443, 446, 1018, 1079
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 9 cut(s) 26, 110, 325, 495, 696, 838, 1000, 1076, 1207
TspGWI ACGGA 3 cut(s) 603, 787, 1172
TspRI CASTG 5 cut(s) 202, 625, 937, 1129, 1196
Vha464I CTTAAG 1 cut(s) 667
XapI RAATTY 4 cut(s) 205, 228, 512, 836
XbaI TCTAGA 1 cut(s) 178
XmiI GTMKAC 3 cut(s) 535, 803, 940
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.