MD04G1183500.v1.1

Transmembrane protein 184A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009384
Physical Location & Seq
Forward (+)
27478389 .. 27482576
4188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1183500.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGGCGGAGTCGACCCCAATCTACCTGAGCATTATTGCTTTTTTCTGTACTGTTGGAGCCATGGCTTTGGCCATTATGCACATCTACAGACACCTCATGAATTACACAGAACCGACTTATCAGAGATACATTGTCCGCATCATCTTCATGGTCCCGGTATATGCATTGATGTCGTTCTTATCCCTTGTTAATCCTGTGGGTTCAATCTACTTTAATTCCATTAGAGAAGTCTATGAAGCTTGGGTCATTTATAATTTTTTATCGCTGTGCTTGGCATGGGTTGGCGGCCCAGGATCTGTTGTTGTAAGTCTAAGTGGTAGGGTTCTAAAGCCTTCCGTGGTTCTGATGACATGTTGCCTCCCTCCCATACCCCTGGATGGGCGCTTTATACGTAGGTGCAAACAAGGATGTTTGCAGTTTGTGATCCTGAAGCCTGTATTAGCTGTTGTTACACTCATACTTTATGCAAAGGGGAAATATGCAGATGGAAATTTCAGTCCAACACAGTCATACCTGTATCTCACTATTATCTACACTATCTCATACACAACAGCTCTCTATGCTCTGGCATTGTTTTACTTTGCTTGCAGAGATCTACTCCAGCCTTTCAATCCAGTTCCAAAGTTTATTATAATCAAATCTGTGGTTTTCCTCACTTATTGGCAGGGTGTGTTGGTCTTCCTTGCTGCGAAATCTGGGTTAATAAAGAATGCAGAGAATGCTGCTCGGTACCAAGATTTCATAATATGTGTTGAGATGCTTATTGCTGCTATTGGTCATTTATATGCATTCCCTTACAAGGAGTATGCTGGTGCAAATATTGGCGGGCCTCGAGGACTGACTGGAAGCCTCTCACATGCCGTAAAGTTGAATGACTTTTACCATGATACAGTCCACCAGTTTGCCCCTACTTACCATGATTATGTACTCTACAACCCTAGTGAGGGTGACGAGGGAAAGAGGAAGTACCGGTCACGCACCTTTGTGCCAACCGGCCCAGAGATGGATACTGTCAGAAGAAACAAGAACATGTTTGGGAACAAGATAGATGACATACAGCTTTCAAGTCTTTCATCTTCTAGCTCAAGCACTCCGGATAGTTCTGGTCCAGTACCCGAGTCTGCAAGTTCAGATGCAATGAAGTCTTCCCTTCTTGTTGATACCTCAAATTCTGCATCAGTGCCGTATGACATGTCACTCATCGACTTAGACTTGAACAGTTACCCCGCGAAGGTTCCCTCAGCAAATGAAGGTGGCACTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Solute_trans_a

Protein Analysis

422

Amino Acids

46.74

Weight (kDa)

8.6

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 10 - 269 5.2e-92 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 252, 632
AbsI CCTCGAGG 1 cut(s) 831
Acc65I GGTACC 1 cut(s) 729
AccB1I GGYRCC 1 cut(s) 729
AccI GTMKAC 1 cut(s) 11
AccII CGCG 1 cut(s) 1229
AccIII TCCGGA 1 cut(s) 1093
AciI CCGC 5 cut(s) 5, 136, 285, 825, 1227
AclWI GGATC 2 cut(s) 301, 418
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 2 cut(s) 490, 1168
AcuI CTGAAG 1 cut(s) 449
AdeI CACNNNGTG 1 cut(s) 1263
AfaI GTAC 5 cut(s) 49, 731, 927, 968, 1113
AfiI CCNNNNNNNGG 7 cut(s) 377, 378, 799, 943, 944, 1003, 1231
AflIII ACRYGT 3 cut(s) 350, 1029, 1191
AgeI ACCGGT 1 cut(s) 969
AgsI TTSAA 5 cut(s) 204, 610, 871, 1065, 1216
AjnI CCWGG 2 cut(s) 289, 372
AleI CACNNNNGTG 1 cut(s) 983
AluBI AGCT 5 cut(s) 239, 443, 554, 1060, 1083
AluI AGCT 5 cut(s) 239, 443, 554, 1060, 1083
AlwI GGATC 2 cut(s) 301, 418
AlwNI CAGNNNCTG 1 cut(s) 296
Ama87I CYCGRG 2 cut(s) 831, 1115
Aor13HI TCCGGA 1 cut(s) 1093
AoxI GGCC 4 cut(s) 69, 286, 827, 994
ApeKI GCWGC 3 cut(s) 686, 722, 767
ApoI RAATTY 2 cut(s) 490, 1168
AsiGI ACCGGT 1 cut(s) 969
Asp718I GGTACC 1 cut(s) 729
AspLEI GCGC 1 cut(s) 384
AspS9I GGNCC 5 cut(s) 151, 287, 827, 995, 1106
AsuC2I CCSGG 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 959
AvaI CYCGRG 2 cut(s) 831, 1115
AvaII GGWCC 2 cut(s) 151, 1106
BalI TGGCCA 1 cut(s) 71
BanI GGYRCC 1 cut(s) 729
BbsI GAAGAC 2 cut(s) 670, 1137
BbvCI CCTCAGC 1 cut(s) 1240
BbvI GCAGC 3 cut(s) 673, 709, 754
BccI CCATC 3 cut(s) 371, 479, 997
BceAI ACGGC 2 cut(s) 845, 1168
BcgI CGANNNNNNTGC 2 cut(s) 153, 187
BciT130I CCWGG 2 cut(s) 291, 374
BciVI GTATCC 1 cut(s) 1000
BcnI CCSGG 1 cut(s) 155
BfaI CTAG 3 cut(s) 939, 1080, 1260
BfmI CTRYAG 1 cut(s) 85
BfoI RGCGCY 1 cut(s) 385
BfuI GTATCC 1 cut(s) 1000
BglII AGATCT 1 cut(s) 592
BisI GCNGC 4 cut(s) 286, 687, 723, 768
BlsI GCNGC 4 cut(s) 287, 688, 724, 769
Bme1390I CCNGG 3 cut(s) 155, 291, 374
Bme18I GGWCC 2 cut(s) 151, 1106
BmeT110I CYCGRG 2 cut(s) 831, 1115
BmgT120I GGNCC 5 cut(s) 151, 287, 827, 995, 1106
BmiI GGNNCC 4 cut(s) 58, 153, 731, 1236
BmrFI CCNGG 3 cut(s) 155, 291, 374
BmsI GCATC 4 cut(s) 147, 747, 1123, 1184
BpiI GAAGAC 2 cut(s) 670, 1137
BplI GAGNNNNNCTC 2 cut(s) 582, 614
BpmI CTGGAG 1 cut(s) 584
Bpu10I CCTNAGC 2 cut(s) 26, 1240
BpuEI CTTGAG 1 cut(s) 1069
BpuMI CCSGG 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 392
BsaJI CCNNGG 4 cut(s) 60, 289, 336, 372
BsaWI WCCGGW 2 cut(s) 969, 1093
Bsc4I CCNNNNNNNGG 7 cut(s) 377, 378, 799, 943, 944, 1003, 1231
Bse118I RCCGGY 2 cut(s) 969, 992
Bse1I ACTGG 4 cut(s) 614, 847, 898, 1109
Bse3DI GCAATG 1 cut(s) 1143
BseAI TCCGGA 1 cut(s) 1093
BseBI CCWGG 2 cut(s) 291, 374
BseDI CCNNGG 4 cut(s) 60, 289, 336, 372
BseGI GGATG 2 cut(s) 382, 413
BseLI CCNNNNNNNGG 7 cut(s) 377, 378, 799, 943, 944, 1003, 1231
BseMI GCAATG 1 cut(s) 1143
BseMII CTCAG 2 cut(s) 17, 1254
BseNI ACTGG 4 cut(s) 614, 847, 898, 1109
BseXI GCAGC 3 cut(s) 673, 709, 754
Bsh1236I CGCG 1 cut(s) 1229
BshFI GGCC 4 cut(s) 71, 288, 829, 996
BshNI GGYRCC 1 cut(s) 729
BshTI ACCGGT 1 cut(s) 969
BsiHKCI CYCGRG 2 cut(s) 831, 1115
BsiSI CCGG 4 cut(s) 155, 970, 993, 1094
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 7 cut(s) 377, 378, 799, 943, 944, 1003, 1231
BsmFI GGGAC 1 cut(s) 137
BsmI GAATGC 3 cut(s) 715, 724, 788
BsnI GGCC 4 cut(s) 71, 288, 829, 996
BsoBI CYCGRG 2 cut(s) 831, 1115
Bsp13I TCCGGA 1 cut(s) 1093
Bsp143I GATC 3 cut(s) 293, 423, 592
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 5 cut(s) 5, 136, 285, 825, 1227
BspANI GGCC 4 cut(s) 71, 288, 829, 996
BspCNI CTCAG 2 cut(s) 18, 1253
BspEI TCCGGA 1 cut(s) 1093
BspFNI CGCG 1 cut(s) 1229
BspHI TCATGA 1 cut(s) 96
BspLI GGNNCC 4 cut(s) 58, 153, 731, 1236
BspPI GGATC 2 cut(s) 301, 418
BspT107I GGYRCC 1 cut(s) 729
BsrDI GCAATG 1 cut(s) 1143
BsrFI RCCGGY 2 cut(s) 969, 992
BsrI ACTGG 4 cut(s) 614, 847, 898, 1109
BssAI RCCGGY 2 cut(s) 969, 992
BssECI CCNNGG 4 cut(s) 60, 289, 336, 372
BssMI GATC 3 cut(s) 293, 423, 592
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 291, 374
Bst4CI ACNGT 5 cut(s) 52, 507, 892, 1012, 1220
BstAPI GCANNNNNTGC 1 cut(s) 719
BstBAI YACGTR 1 cut(s) 392
BstC8I GCNNGC 2 cut(s) 586, 827
BstDEI CTNAG 4 cut(s) 26, 311, 1207, 1240
BstDSI CCRYGG 2 cut(s) 60, 336
BstF5I GGATG 2 cut(s) 382, 413
BstFNI CGCG 1 cut(s) 1229
BstH2I RGCGCY 1 cut(s) 385
BstHHI GCGC 1 cut(s) 384
BstKTI GATC 3 cut(s) 296, 426, 595
BstMBI GATC 3 cut(s) 293, 423, 592
BstMWI GCNNNNNNNGC 2 cut(s) 560, 719
BstNI CCWGG 2 cut(s) 291, 374
BstNSI RCATGY 4 cut(s) 354, 860, 1033, 1195
BstSCI CCNGG 3 cut(s) 153, 289, 372
BstSFI CTRYAG 1 cut(s) 85
BstSNI TACGTA 1 cut(s) 392
BstUI CGCG 1 cut(s) 1229
BstV1I GCAGC 3 cut(s) 673, 709, 754
BstV2I GAAGAC 2 cut(s) 670, 1137
BstX2I RGATCY 2 cut(s) 293, 592
BstXI CCANNNNNNTGG 2 cut(s) 67, 373
BstYI RGATCY 2 cut(s) 293, 592
BsuI GTATCC 1 cut(s) 1000
BsuRI GGCC 4 cut(s) 71, 288, 829, 996
BtgI CCRYGG 2 cut(s) 60, 336
BtsCI GGATG 2 cut(s) 382, 413
BtsIMutI CAGTG 1 cut(s) 1185
Cac8I GCNNGC 2 cut(s) 586, 827
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 1 cut(s) 96
CfoI GCGC 1 cut(s) 384
Cfr10I RCCGGY 2 cut(s) 969, 992
Cfr13I GGNCC 5 cut(s) 151, 287, 827, 995, 1106
Csp6I GTAC 5 cut(s) 48, 730, 926, 967, 1112
CspAI ACCGGT 1 cut(s) 969
CviQI GTAC 5 cut(s) 48, 730, 926, 967, 1112
DdeI CTNAG 4 cut(s) 26, 311, 1207, 1240
DpnI GATC 3 cut(s) 295, 425, 594
DpnII GATC 3 cut(s) 293, 423, 592
DraIII CACNNNGTG 1 cut(s) 1263
EaeI YGGCCR 1 cut(s) 69
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 392
Eco130I CCWWGG 1 cut(s) 60
Eco47I GGWCC 2 cut(s) 151, 1106
Eco57I CTGAAG 1 cut(s) 449
Eco88I CYCGRG 2 cut(s) 831, 1115
EcoRII CCWGG 2 cut(s) 289, 372
EcoT14I CCWWGG 1 cut(s) 60
EcoT22I ATGCAT 2 cut(s) 166, 790
ErhI CCWWGG 1 cut(s) 60
FaqI GGGAC 1 cut(s) 137
FauI CCCGC 2 cut(s) 818, 1234
FblI GTMKAC 1 cut(s) 11
Fnu4HI GCNGC 4 cut(s) 286, 687, 723, 768
FokI GGATG 2 cut(s) 389, 420
Fsp4HI GCNGC 4 cut(s) 286, 687, 723, 768
FspBI CTAG 3 cut(s) 939, 1080, 1260
GlaI GCGC 1 cut(s) 383
GluI GCNGC 4 cut(s) 286, 687, 723, 768
GsuI CTGGAG 1 cut(s) 584
HaeII RGCGCY 1 cut(s) 385
HaeIII GGCC 4 cut(s) 71, 288, 829, 996
HapII CCGG 4 cut(s) 155, 970, 993, 1094
HhaI GCGC 1 cut(s) 384
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 2 cut(s) 8, 1118
HpaII CCGG 4 cut(s) 155, 970, 993, 1094
HphI GGTGA 1 cut(s) 959
Hpy166II GTNNAC 2 cut(s) 12, 895
Hpy188I TCNGA 4 cut(s) 123, 345, 1016, 1132
Hpy188III TCNNGA 3 cut(s) 97, 427, 1094
Hpy8I GTNNAC 2 cut(s) 12, 895
HpyAV CCTTC 4 cut(s) 342, 1160, 1225, 1244
HpyCH4III ACNGT 5 cut(s) 52, 507, 892, 1012, 1220
HpyCH4IV ACGT 1 cut(s) 391
HpyF10VI GCNNNNNNNGC 2 cut(s) 560, 719
HpyF3I CTNAG 4 cut(s) 26, 311, 1207, 1240
HpySE526I ACGT 1 cut(s) 391
HspAI GCGC 1 cut(s) 382
Kpn2I TCCGGA 1 cut(s) 1093
KpnI GGTACC 1 cut(s) 733
Kzo9I GATC 3 cut(s) 293, 423, 592
LmnI GCTCC 1 cut(s) 56
Lsp1109I GCAGC 3 cut(s) 673, 709, 754
LweI GCATC 4 cut(s) 147, 747, 1123, 1184
MaeI CTAG 3 cut(s) 939, 1080, 1260
MaeII ACGT 1 cut(s) 391
MaeIII GTNAC 5 cut(s) 448, 947, 972, 1194, 1220
MalI GATC 3 cut(s) 295, 425, 594
MboI GATC 3 cut(s) 293, 423, 592
MboII GAAGA 5 cut(s) 136, 670, 1029, 1068, 1137
MflI RGATCY 2 cut(s) 293, 592
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 5 cut(s) 100, 214, 253, 490, 1168
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 2 cut(s) 17, 1127
MmeI TCCRAC 2 cut(s) 34, 524
Mox20I TGGCCA 1 cut(s) 71
Mph1103I ATGCAT 2 cut(s) 166, 790
MroI TCCGGA 1 cut(s) 1093
MscI TGGCCA 1 cut(s) 71
MseI TTAA 3 cut(s) 189, 213, 701
MslI CAYNNNNRTG 4 cut(s) 146, 783, 921, 983
Msp20I TGGCCA 1 cut(s) 71
MspI CCGG 4 cut(s) 155, 970, 993, 1094
MspR9I CCNGG 3 cut(s) 155, 291, 374
Mva1269I GAATGC 3 cut(s) 715, 724, 788
MvaI CCWGG 2 cut(s) 291, 374
MvnI CGCG 1 cut(s) 1229
MwoI GCNNNNNNNGC 2 cut(s) 560, 719
NciI CCSGG 1 cut(s) 155
NcoI CCATGG 1 cut(s) 60
NdeII GATC 3 cut(s) 293, 423, 592
NlaIV GGNNCC 4 cut(s) 58, 153, 731, 1236
NmuCI GTSAC 3 cut(s) 947, 972, 1194
NsiI ATGCAT 2 cut(s) 166, 790
NspI RCATGY 4 cut(s) 354, 860, 1033, 1195
OliI CACNNNNGTG 1 cut(s) 983
PaeR7I CTCGAG 1 cut(s) 831
PagI TCATGA 1 cut(s) 96
PciI ACATGT 3 cut(s) 350, 1029, 1191
PctI GAATGC 3 cut(s) 715, 724, 788
PinAI ACCGGT 1 cut(s) 969
PkrI GCNGC 4 cut(s) 287, 688, 724, 769
PleI GAGTC 2 cut(s) 16, 1126
PpsI GAGTC 2 cut(s) 16, 1126
Ppu21I YACGTR 1 cut(s) 392
PscI ACATGT 3 cut(s) 350, 1029, 1191
PsiI TTATAA 2 cut(s) 252, 632
Psp6I CCWGG 2 cut(s) 289, 372
PspGI CCWGG 2 cut(s) 289, 372
PspN4I GGNNCC 4 cut(s) 58, 153, 731, 1236
PspPI GGNCC 5 cut(s) 151, 287, 827, 995, 1106
PspXI VCTCGAGB 1 cut(s) 831
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 2 cut(s) 293, 592
RsaI GTAC 5 cut(s) 49, 731, 927, 968, 1113
RsaNI GTAC 5 cut(s) 48, 730, 926, 967, 1112
RseI CAYNNNNRTG 4 cut(s) 146, 783, 921, 983
SalI GTCGAC 1 cut(s) 10
SaqAI TTAA 3 cut(s) 189, 213, 701
SatI GCNGC 4 cut(s) 286, 687, 723, 768
Sau3AI GATC 3 cut(s) 293, 423, 592
Sau96I GGNCC 5 cut(s) 151, 287, 827, 995, 1106
SchI GAGTC 2 cut(s) 17, 1127
ScrFI CCNGG 3 cut(s) 155, 291, 374
SfaNI GCATC 4 cut(s) 147, 747, 1123, 1184
SfcI CTRYAG 1 cut(s) 85
Sfr274I CTCGAG 1 cut(s) 831
SinI GGWCC 2 cut(s) 151, 1106
SlaI CTCGAG 1 cut(s) 831
SmiMI CAYNNNNRTG 4 cut(s) 146, 783, 921, 983
SmlI CTYRAG 2 cut(s) 831, 1084
SmoI CTYRAG 2 cut(s) 831, 1084
SnaBI TACGTA 1 cut(s) 392
Sse9I AATT 5 cut(s) 100, 214, 253, 490, 1168
SsiI CCGC 5 cut(s) 5, 136, 285, 825, 1227
SspI AATATT 1 cut(s) 820
SspMI CTAG 3 cut(s) 939, 1080, 1260
StyD4I CCNGG 3 cut(s) 153, 289, 372
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 5 cut(s) 52, 507, 892, 1012, 1220
TaiI ACGT 1 cut(s) 394
TaqI TCGA 3 cut(s) 11, 832, 1203
TasI AATT 5 cut(s) 100, 214, 253, 490, 1168
TatI WGTACW 2 cut(s) 47, 925
TauI GCSGC 1 cut(s) 288
Tru1I TTAA 3 cut(s) 189, 213, 701
Tru9I TTAA 3 cut(s) 189, 213, 701
TscAI CASTG 1 cut(s) 1185
TseFI GTSAC 3 cut(s) 947, 972, 1194
TseI GCWGC 3 cut(s) 686, 722, 767
Tsp45I GTSAC 3 cut(s) 947, 972, 1194
TspDTI ATGAA 7 cut(s) 113, 136, 249, 730, 1062, 1154, 1263
TspGWI ACGGA 1 cut(s) 325
TspRI CASTG 1 cut(s) 1185
VpaK11BI GGWCC 2 cut(s) 151, 1106
XapI RAATTY 2 cut(s) 490, 1168
XceI RCATGY 4 cut(s) 354, 860, 1033, 1195
XhoI CTCGAG 1 cut(s) 831
XmiI GTMKAC 1 cut(s) 11
XspI CTAG 3 cut(s) 939, 1080, 1260
Zsp2I ATGCAT 2 cut(s) 166, 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.