Rorug03G0033300

Transmembrane protein 184A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0009384
Physical Location & Seq
Forward (+)
2660403 .. 2668182
7780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0033300.1

Sequence Viewer

Length: 4914 bp
ATGGCTTCCTCTTCTGTTTCTTCTTTTGTCATTCCCCCAAAAGAAAAGTATGATGTCTTTCTTAGTTTCAGAGGCAAGGACACTCGCAAGACTTTTACCAGCCATCTTCATGCTGCTTTGATAAGGAGGAAAGTGGAAACCTACATTGATTACAGACTTGAGAGAGGAGATGAAGTTGGACCTGCCCTTCTAAAAGCAATCAAGGAATCAAAGATTTCCGTGATCATTTTCTCAAAAGACTATGCTTCTTCCACATGGTGCTTGGATGAACTTGTTCATATACTGGAATGCAGGGAAAAACATGGACAATGTGTTATACCTATTTTTTACGAAATAGATCCATCACATGTACGCTACCAGACCGAAAGTTATAAGACTGCATTTGCTAAACATGAAACAAGGTTGAAGGACGAGACGGACAAGGTGGCCAAGTGGAAGGAAGCTTTAGTAAAAGCAGCCAATCTGTCTGGGTCTGATTCAGCTTCAAGAACTGTTCGGCATGATTCGGATTTAGTTGAGCAAGTCGTCAGAGATGTGTTGGCTAAATTGAATCGTAAATCATCAAGCGACTTAACTGGTTTGATTGGAGCTGAAGACCGCATTAAGAAAATTTTATTGGAATTAGACATCATCCCAGAAGATGTTCGTGTTCGCTCTGTAGTTATCTGGGGTATGGGTGGTATTGGCAAGACCACGCTTGCTGATGCTGTATATCATGGACTCTCTTCTCAATTCGAAGCTCACTGTTTTCTTGCAAATGTTAGGGAACAAGCAGGTACCAAAGGAGGTTTCTCTCCTGAACTGTATCTTTTGCGAAATGAACTTCTTGGAGAATTACTAGGGGACAGGAGTTTAGCTATCCATACTCGAACTATAGGTGCTGTTGACAAAGAGAGGCTCCGCCGTACAAAAGTCCTTGTTGTTCTTGACGACGTGAATGATTCAAGCCAATTAACATTTTTAGCTGGAGAAGTTCCATTTGGCCCGGGAAGTAGAATAATTATAACAACTCGAGATATGCAACCAGTTAAGGAGGCTCTACTAGTGAAGAAAGTAGCTGACCATGATGTTAAGATCTACAAGGCAGAGGAATTAAATGGTGATGAATATCTTCAGCTCTTCCATTCAGATGCTGCCACTCATTGTGCAGAAGATCTTTTAGAGAAGCAGGTGGCAGATCATGCTGCAGGAATTCCATTAGCCCTTAACATTTTGCGTTCATTATTCCTTCGATGCAAGAGCAAAGAAGAACAGGAACTGTTGTGGGAAAAATTGAAAAAGTTTCCCAACAAAAAACTTCAGAATGTGTATAGAGCAAGTTGTGATGGATTAGAAGAAAACGAAAGGGAGATATTCCTTGATATTGCATGTCTTCACAGAAGGGAGAAACTTTGCGATGCAAAAAGAATGTTAGCTGCCTGTGGTTTATTTCCGGATGATGGAATTGACATTCTCATTGATATGTCTCTCATATCAATTAAAGACAACTGCATATGGATGCACGATGTGATCCAAGAAATGGGTTGGGAGATTATCCGCGAAGAAAGTACTGAAGATCCAGGAAAGCGCAGTAGGTTGCACAATGGTGAGGATGTCTGTCATGTATTGAAAAGGAATACGGGAACTGCAACAGTTCAAAGCATTTCCAGCAGAAATTGTGTTAACAGTCTAACGTTGGATCCTCAAGCTTTTACACGGATGCATAACTTAAGATTCCTCGTGCTTAGATTTATACATCTTGACCACAAGGCAAATCTTGAGTATCTTCCTGATGCCCTTCGCTATCTCTTTTGGCATAGTTACCCTTTGAAATATTTGCCATCAATGTTTTCTCCCAAAAATCTTGTAGAGCTCAATATGCCCTGGAGCAAACTGAAGAGACTTTGGAGAAAAGGCCAGAATCCTGAGAACTTGAAAAGGATCGATCTTAGTTACTCCATGGATCTGGTTGAAGTTGCAGATCTGTCAAAAAGTGTAAACATTGAGAGTATAGATTTTCAATGCTGTAAAAGTTTGGTTAAAGTTCCGGATTTGTCAAAGAGTCGATACATTGAGAGTATAAATCTTCAAGGCTGTATAAGTTTGGTTAAAGTCCCAGACCTGTCAAAGAGTGTAAATATTCAGAGTATAAATCTTCAAGGCTGTGAAAGTTTAGTTCAAGTTCCTCCGTATTTTCAAAATTTTACCGAGCTTACTCATCTGAATTTGAGAGGTTGCTCGAAAATTAGAAGTATTCCAAAGATACCAAGCACGGTGGAATTCTTGGATTTGAGTGAAACTGCAATAGAAGAATTGCCTTCATCACTTTGGTCTCTTGAAAGACTTGTTAGATTGGATCTTAAAGACTGTGGGGCCCATTCGCATTTTTCGAGACATCCATGGAAGATGAAATTCCTCAATTATCTTTCTTTGAGTAAGACAAAGATAGAAACAGTGCCCTCGTCAATTTTGTGTATGACTTGGTTAATTCAACTTGACCTGAGTTTCTGTGGTTGCCTTGTGAGTCTCCCAACCGACATTTGTAAGTTGAAACCTCTTGAGAAACTTGATCTATCTGGTTGCTACAGATTCACTCACTTCCCAGAAATTTTGAAGCCTATGGAACGTCTGGCATATCTTGGTTTAAGTGGGACAAAAATTGAAGAGCTACCATCATCGATTGGGAATCTTGTTGTGCTTAAGACTTTAGATTTATCAAACTGCACAAGCCTTGAATCACTCCCAAACAGCATCTACAATTTAAAGTTTCTCGAGTGGTTCTCACTTAGTGGCTGTTACAGGCTAAAAGAATTGCCTCTCTCGTTCATTTTGTGCTCTTTGATAAATCTAAACCTCGGAGGCTGCAAATTGTTAGAGGAAATTCCCGATTGCTTTACTAGCTTTCCCGCATTGCAAGTCTTAAACCTAAGTGGAACCATGATTGAGACCATACCTCCCACCATCAAACAAGTTTTTGAGCTTAAGTCTCTGAGACTTGAAGGGTGCAAGCGGCTTCAATCTTTGCCAGTGCTCCCATGTCTTCTAGAAGAATTGGTTGCAACAGAGTGCACGAGACTTGAGCAAGTGTCAGTTTCAGTGACCGTCCAGACACAAGGTTTAGATCATAAACTTTCTGGGAAGAGAACCGAGAGACATACATATTTAGCTTGCGTTAATTTGGATAAAAATGCAATGAGCAACATAACGGATGACGCACACTTCAGAATTATGCGAATGGCGACTGCATGTAAACTTAAACGTTTATCATCAGGTAAAGTAACGTTATTGTGTCCGGGAAATGACATTCCAATATGGTTTAGCTGTCAAACGGAGGGATCTTCAATGAATATTAAGCTTCCTCTGCACTGGTCTGATAATTCGAACTTCTTGGGTACTGCTCTGTCTTCTGTTTCTGCAATCCATGCAAATCCTCATCCTCGTCTTGAGTATCAATGTGAGATGATTCTCAAAACCAACAATGGTGAAACCTATAGTGTCAATTTGGGAAGTGCAGAGCACTTCGGGATTGGGGGCCTGACGGCTACAACAGATCACGTGTTCGTGTGGTATAATAAAGTCCATGAAATCTCAGATGAAGCAAAATGGTCGGTGGAAGCCTCTTTTGACTTCTATACAGAAGTCGATGGTAAACGGTGGTATAACGTGAAAAGGTGTGGGGTATGCTTTCTCTATGGCCAAGGCCAAGATGATGATGCTCTGAAATTTGAAGTCATTTGTCCACAACAAGTTGCTACAACTAGGAGACATCGCGGCCTCTTTGAGGATGAAACTAGTACTAGTGGAAGTACTCTAATGTGGAGACACCGCGGCCGCTTTGAGGATGAAGCTGGTGGAAGTCAAATGGCTGCTCCAACTGCGCTGTCGAAGAGACATGCTGATGGTTCATTGCCAATTGAAGATTTCAACATAGTACTTGGAAATCAGAGTGGGAAGAAAGTACAAATGGGATCAGAGACTGATCAGCGGGCCATCATGATGAGCCTGATGTTACATACCAATGCAAAGCAACTGATTAAAGCACAAAATTACCAAGATGCAATGGAAGTGCTCAGCATGGAGGAGGAGGCTTTCTCTCTCTGCCATCCATCGGTTATTAAGGAATTCGCCTTGAGAAAGCCAGAGAGGGTCTTGAACGAGCTCATGGAAAGGACTCTTCTCGTGTTAGACTCCTTCAGGCAGGTTGTTATCCAGAGCATGCACTGTGACTACTTCTACTACATATCTGAATGTTTGTCTACACTACGCAGATATTTGATACTGGAGCTGCTGGAAGGGGTGGCGGCGTATCACAGTGGTCAGGTTGATAAGTCAATGACGGTGTTGACTTCTGTACAAGAGAAGTTCACCCAGGTGCTGGATGAATTTTTGGTTGTGGATTCCAGCTTCAGGTGCTGGATGAATCTCTCTCTCTCTTGTGATGATCATGGGCTTCAGAGAACGAGATACAAAAAGGGCACTGGGGATGAGCAATCAAGATGTTTCCATCAGCCCTGTACAGTCGTTAGTCATCCTAAGAGTTTCTTGACTTTGCAGCAACATCTTGAATCAAGGAAAAGTAAACTACAGTGCCAAGCAGTAGATGCTTCAGTTGAAGCTTTTGTACGCATGGCCTTTGACAGATCATTAGCACTGACTTTGCGTTCAGCAGTCTTAGCTTTGCAGGGTGGAGGGACAATGGACCAAGCAATCTGTCAACTGCTCTCGGGACAGGCACCAAACCCCACAGATGCTGCTAACCAATCTGAGGCAATTCCTGCAGTCAATGCTAACAACAATAGTCAATCTGATGTTGCAATTGACATTGCAAATTCTCTTGCGTCAGTGCTTGATAATCAAAATGGTCAAGTTGGAGGTTTTTCAACTTCTAGTAATGCATCAGAGCGTGATTCAGAAAGGGAAAAGCCAACTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Solute_trans_a

Protein Analysis

1637

Amino Acids

184.42

Weight (kDa)

6.37

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 193 1.3e-53 TIR domain
TIR_2 PF13676 19 - 114 5.6e-13 TIR domain
NB-ARC PF00931 214 - 375 7.6e-11 NB-ARC domain
WHD_ROQ1 PF23282 445 - 516 2.8e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 778 - 870 3e-07 Leucine-rich repeat region
LRR_13 PF23286 844 - 1029 9.9e-17 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 849 - 928 7.3e-12 Leucine-rich repeat region
C-JID PF20160 1090 - 1226 8.1e-12 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 372, 1004
AarI CACCTGC 1 cut(s) 1159
Acc36I ACCTGC 4 cut(s) 190, 764, 1159, 4168
Acc65I GGTACC 1 cut(s) 776
AccB1I GGYRCC 2 cut(s) 776, 4711
AccB7I CCANNNNNTGG 2 cut(s) 1519, 4354
AccI GTMKAC 1 cut(s) 4235
AccII CGCG 3 cut(s) 1539, 3750, 3807
AccIII TCCGGA 2 cut(s) 1432, 2026
AclI AACGTT 3 cut(s) 1673, 3238, 3260
AcoI YGGCCR 3 cut(s) 426, 3673, 3808
AcvI CACGTG 1 cut(s) 3535
AdeI CACNNNGTG 3 cut(s) 258, 1507, 2768
AfiI CCNNNNNNNGG 8 cut(s) 1439, 1519, 3760, 3817, 4087, 4354, 4386, 4744
AflII CTTAAG 3 cut(s) 1708, 2678, 2960
AflIII ACRYGT 2 cut(s) 346, 3534
AhlI ACTAGT 3 cut(s) 1042, 3770, 3776
AjiI CACGTC 1 cut(s) 934
AjnI CCWGG 3 cut(s) 1558, 1862, 4347
AjuI GAANNNNNNNTTGG 6 cut(s) 599, 631, 773, 805, 963, 995
AleI CACNNNNGTG 2 cut(s) 1584, 4349
Alw21I GWGCWC 7 cut(s) 1854, 2816, 3012, 3050, 3498, 4050, 4140
Alw44I GTGCAC 1 cut(s) 3046
AlwNI CAGNNNCTG 6 cut(s) 1133, 1258, 3956, 4354, 4392, 4730
Ama87I CYCGRG 4 cut(s) 985, 1011, 2750, 4702
Aor13HI TCCGGA 2 cut(s) 1432, 2026
ApaI GGGCCC 1 cut(s) 2356
ApaLI GTGCAC 1 cut(s) 3046
Asp700I GAANNNNTTC 3 cut(s) 273, 642, 1110
Asp718I GGTACC 1 cut(s) 776
AspLEI GCGC 2 cut(s) 1569, 3859
AspS9I GGNCC 7 cut(s) 179, 983, 2352, 2353, 3511, 3966, 4678
AsuC2I CCSGG 3 cut(s) 986, 987, 3273
AsuHPI GGTGA 4 cut(s) 1112, 1598, 3473, 4336
AsuII TTCGAA 2 cut(s) 735, 3359
AvaI CYCGRG 4 cut(s) 985, 1011, 2750, 4702
AvaII GGWCC 2 cut(s) 179, 4678
BaeGI GKGCMC 4 cut(s) 2356, 2439, 3050, 4457
BalI TGGCCA 2 cut(s) 428, 3675
BamHI GGATCC 1 cut(s) 1678
BanI GGYRCC 2 cut(s) 776, 4711
BanII GRGCYC 3 cut(s) 1854, 2356, 4140
BarI GAAGNNNNNNTAC 2 cut(s) 4584, 4616
BauI CACGAG 3 cut(s) 1718, 3049, 4157
BbrPI CACGTG 1 cut(s) 3535
BbsI GAAGAC 4 cut(s) 600, 1364, 3011, 3375
Bbv12I GWGCWC 7 cut(s) 1854, 2816, 3012, 3050, 3498, 4050, 4140
BceAI ACGGC 2 cut(s) 888, 3534
BcgI CGANNNNNNTGC 2 cut(s) 3567, 3601
BciT130I CCWGG 3 cut(s) 1560, 1864, 4349
BclI TGATCA 3 cut(s) 222, 3958, 4420
BcnI CCSGG 3 cut(s) 986, 987, 3273
BcuI ACTAGT 3 cut(s) 1042, 3770, 3776
BfaI CTAG 8 cut(s) 839, 1043, 2877, 3023, 3738, 3771, 3777, 4866
BfmI CTRYAG 7 cut(s) 657, 873, 1185, 2563, 3469, 4562, 4755
BfrI CTTAAG 3 cut(s) 1708, 2678, 2960
BfuAI ACCTGC 4 cut(s) 190, 764, 1159, 4168
BglII AGATCT 3 cut(s) 1074, 1153, 1960
BlpI GCTNAGC 1 cut(s) 4049
BmcAI AGTACT 4 cut(s) 1549, 3775, 3787, 3912
Bme1390I CCNGG 6 cut(s) 986, 987, 1560, 1864, 3273, 4349
Bme18I GGWCC 2 cut(s) 179, 4678
BmeT110I CYCGRG 4 cut(s) 985, 1011, 2750, 4702
BmgBI CACGTC 1 cut(s) 934
BmgT120I GGNCC 7 cut(s) 179, 983, 2352, 2353, 3511, 3966, 4678
BmiI GGNNCC 8 cut(s) 778, 899, 1680, 2353, 2354, 2914, 3512, 4713
BmrFI CCNGG 6 cut(s) 986, 987, 1560, 1864, 3273, 4349
BmrI ACTGGG 1 cut(s) 4467
BmuI ACTGGG 1 cut(s) 4467
BpiI GAAGAC 4 cut(s) 600, 1364, 3011, 3375
BplI GAGNNNNNCTC 4 cut(s) 2744, 2776, 4055, 4087
BpmI CTGGAG 3 cut(s) 987, 1885, 4280
Bpu1102I GCTNAGC 1 cut(s) 4049
Bpu14I TTCGAA 2 cut(s) 735, 3359
BpuEI CTTGAG 7 cut(s) 179, 1668, 1778, 2558, 3077, 3443, 4129
BpuMI CCSGG 3 cut(s) 986, 987, 3273
Bsa29I ATCGAT 2 cut(s) 1923, 2657
BsaAI YACGTR 1 cut(s) 3535
BsaBI GATNNNNATC 1 cut(s) 1107
BsaI GGTCTC 2 cut(s) 2316, 2918
BsaJI CCNNGG 8 cut(s) 985, 1862, 1938, 2378, 2833, 3676, 3805, 4347
BsaWI WCCGGW 2 cut(s) 1432, 2026
BsaXI ACNNNNNCTCC 4 cut(s) 159, 189, 2917, 2947
Bsc4I CCNNNNNNNGG 8 cut(s) 1439, 1519, 3760, 3817, 4087, 4354, 4386, 4744
Bse1I ACTGG 7 cut(s) 288, 580, 1025, 3005, 3350, 4263, 4462
Bse3DI GCAATG 5 cut(s) 2888, 3177, 3884, 4044, 4800
Bse8I GATNNNNATC 1 cut(s) 1107
BseAI TCCGGA 2 cut(s) 1432, 2026
BseBI CCWGG 3 cut(s) 1560, 1864, 4349
BseCI ATCGAT 2 cut(s) 1923, 2657
BseDI CCNNGG 8 cut(s) 985, 1862, 1938, 2378, 2833, 3676, 3805, 4347
BseJI GATNNNNATC 1 cut(s) 1107
BseLI CCNNNNNNNGG 8 cut(s) 1439, 1519, 3760, 3817, 4087, 4354, 4386, 4744
BseMI GCAATG 5 cut(s) 2888, 3177, 3884, 4044, 4800
BseMII CTCAG 6 cut(s) 1896, 2471, 2960, 3582, 4063, 4734
BseNI ACTGG 7 cut(s) 288, 580, 1025, 3005, 3350, 4263, 4462
BseRI GAGGAG 3 cut(s) 180, 4073, 4076
BseSI GKGCMC 4 cut(s) 2356, 2439, 3050, 4457
BseX3I CGGCCG 1 cut(s) 3808
BsgI GTGCAG 4 cut(s) 1167, 2686, 3326, 3510
Bsh1236I CGCG 3 cut(s) 1539, 3750, 3807
Bsh1285I CGRYCG 1 cut(s) 3811
BshNI GGYRCC 2 cut(s) 776, 4711
BshVI ATCGAT 2 cut(s) 1923, 2657
BsiEI CGRYCG 1 cut(s) 3811
BsiHKAI GWGCWC 7 cut(s) 1854, 2816, 3012, 3050, 3498, 4050, 4140
BsiHKCI CYCGRG 4 cut(s) 985, 1011, 2750, 4702
BsiSI CCGG 4 cut(s) 986, 1433, 2027, 3272
BslFI GGGAC 5 cut(s) 857, 2078, 2644, 4684, 4719
BslI CCNNNNNNNGG 8 cut(s) 1439, 1519, 3760, 3817, 4087, 4354, 4386, 4744
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 5 cut(s) 857, 2078, 2644, 4684, 4719
BsmI GAATGC 1 cut(s) 293
Bso31I GGTCTC 2 cut(s) 2316, 2918
BsoBI CYCGRG 4 cut(s) 985, 1011, 2750, 4702
Bsp119I TTCGAA 2 cut(s) 735, 3359
Bsp120I GGGCCC 1 cut(s) 2352
Bsp13I TCCGGA 2 cut(s) 1432, 2026
Bsp1407I TGTACA 2 cut(s) 4330, 4493
Bsp1720I GCTNAGC 1 cut(s) 4049
Bsp19I CCATGG 2 cut(s) 1938, 2378
BspCNI CTCAG 6 cut(s) 1897, 2472, 2961, 3581, 4062, 4735
BspDI ATCGAT 2 cut(s) 1923, 2657
BspEI TCCGGA 2 cut(s) 1432, 2026
BspFNI CGCG 3 cut(s) 1539, 3750, 3807
BspHI TCATGA 1 cut(s) 3972
BspLI GGNNCC 8 cut(s) 778, 899, 1680, 2353, 2354, 2914, 3512, 4713
BspMAI CTGCAG 2 cut(s) 1189, 4759
BspMI ACCTGC 4 cut(s) 190, 764, 1159, 4168
BspQI GCTCTTC 2 cut(s) 1124, 2637
BspT104I TTCGAA 2 cut(s) 735, 3359
BspT107I GGYRCC 2 cut(s) 776, 4711
BspTI CTTAAG 3 cut(s) 1708, 2678, 2960
BspTNI GGTCTC 2 cut(s) 2316, 2918
BsrDI GCAATG 5 cut(s) 2888, 3177, 3884, 4044, 4800
BsrGI TGTACA 2 cut(s) 4330, 4493
BsrI ACTGG 7 cut(s) 288, 580, 1025, 3005, 3350, 4263, 4462
BssECI CCNNGG 8 cut(s) 985, 1862, 1938, 2378, 2833, 3676, 3805, 4347
BssSI CACGAG 3 cut(s) 1718, 3049, 4157
BssT1I CCWWGG 3 cut(s) 1938, 2378, 3676
Bst2BI CACGAG 3 cut(s) 1718, 3049, 4157
Bst2UI CCWGG 3 cut(s) 1560, 1864, 4349
Bst6I CTCTTC 8 cut(s) 16, 730, 1124, 1871, 2637, 3113, 3860, 4158
BstAFI CTTAAG 3 cut(s) 1708, 2678, 2960
BstAPI GCANNNNNTGC 5 cut(s) 1181, 3401, 4580, 4754, 4763
BstAUI TGTACA 2 cut(s) 4330, 4493
BstBAI YACGTR 1 cut(s) 3535
BstBI TTCGAA 2 cut(s) 735, 3359
BstC8I GCNNGC 5 cut(s) 699, 2987, 3148, 3966, 4196
BstDSI CCRYGG 3 cut(s) 1938, 2378, 3805
BstENI CCTNNNNNAGG 1 cut(s) 3758
BstFNI CGCG 3 cut(s) 1539, 3750, 3807
BstHHI GCGC 2 cut(s) 1569, 3859
BstMCI CGRYCG 1 cut(s) 3811
BstNI CCWGG 3 cut(s) 1560, 1864, 4349
BstNSI RCATGY 5 cut(s) 350, 1371, 3228, 3875, 4198
BstSCI CCNGG 6 cut(s) 984, 985, 1558, 1862, 3271, 4347
BstSFI CTRYAG 7 cut(s) 657, 873, 1185, 2563, 3469, 4562, 4755
BstSLI GKGCMC 4 cut(s) 2356, 2439, 3050, 4457
BstUI CGCG 3 cut(s) 1539, 3750, 3807
BstV2I GAAGAC 4 cut(s) 600, 1364, 3011, 3375
BstX2I RGATCY 9 cut(s) 337, 1074, 1153, 1555, 1678, 1942, 1960, 2335, 3314
BstXI CCANNNNNNTGG 1 cut(s) 1945
BstYI RGATCY 9 cut(s) 337, 1074, 1153, 1555, 1678, 1942, 1960, 2335, 3314
BstZI CGGCCG 1 cut(s) 3808
Bsu15I ATCGAT 2 cut(s) 1923, 2657
BsuTUI ATCGAT 2 cut(s) 1923, 2657
BtgI CCRYGG 3 cut(s) 1938, 2378, 3805
BtgZI GCGATG 2 cut(s) 1410, 3731
BtrI CACGTC 1 cut(s) 934
BveI ACCTGC 4 cut(s) 190, 764, 1159, 4168
Cac8I GCNNGC 5 cut(s) 699, 2987, 3148, 3966, 4196
CaiI CAGNNNCTG 6 cut(s) 1133, 1258, 3956, 4354, 4392, 4730
CciI TCATGA 1 cut(s) 3972
CciNI GCGGCCGC 1 cut(s) 3808
CfoI GCGC 2 cut(s) 1569, 3859
Cfr13I GGNCC 7 cut(s) 179, 983, 2352, 2353, 3511, 3966, 4678
Cfr42I CCGCGG 1 cut(s) 3808
Cfr9I CCCGGG 1 cut(s) 985
ClaI ATCGAT 2 cut(s) 1923, 2657
CseI GACGC 2 cut(s) 3200, 4806
CspCI CAANNNNNGTGG 2 cut(s) 2235, 2270
DraI TTTAAA 1 cut(s) 2742
DraIII CACNNNGTG 3 cut(s) 258, 1507, 2768
EaeI YGGCCR 3 cut(s) 426, 3673, 3808
EagI CGGCCG 1 cut(s) 3808
Eam1104I CTCTTC 8 cut(s) 16, 730, 1124, 1871, 2637, 3113, 3860, 4158
EarI CTCTTC 8 cut(s) 16, 730, 1124, 1871, 2637, 3113, 3860, 4158
EciI GGCGGA 1 cut(s) 890
Ecl136II GAGCTC 2 cut(s) 1852, 4138
EclXI CGGCCG 1 cut(s) 3808
Eco130I CCWWGG 3 cut(s) 1938, 2378, 3676
Eco24I GRGCYC 3 cut(s) 1854, 2356, 4140
Eco31I GGTCTC 2 cut(s) 2316, 2918
Eco47I GGWCC 2 cut(s) 179, 4678
Eco52I CGGCCG 1 cut(s) 3808
Eco53kI GAGCTC 2 cut(s) 1852, 4138
Eco72I CACGTG 1 cut(s) 3535
Eco88I CYCGRG 4 cut(s) 985, 1011, 2750, 4702
EcoICRI GAGCTC 2 cut(s) 1852, 4138
EcoNI CCTNNNNNAGG 1 cut(s) 3758
EcoO109I RGGNCCY 2 cut(s) 2352, 3511
EcoRI GAATTC 3 cut(s) 1191, 2258, 4100
EcoRII CCWGG 3 cut(s) 1558, 1862, 4347
EcoT14I CCWWGG 3 cut(s) 1938, 2378, 3676
EcoT22I ATGCAT 2 cut(s) 1704, 4876
EcoT38I GRGCYC 3 cut(s) 1854, 2356, 4140
ErhI CCWWGG 3 cut(s) 1938, 2378, 3676
Esp3I CGTCTC 1 cut(s) 407
FalI AAGNNNNNCTT 2 cut(s) 4505, 4537
FaqI GGGAC 5 cut(s) 857, 2078, 2644, 4684, 4719
FauI CCCGC 2 cut(s) 2893, 3957
FauNDI CATATG 1 cut(s) 1493
FbaI TGATCA 3 cut(s) 222, 3958, 4420
FblI GTMKAC 1 cut(s) 4235
FriOI GRGCYC 3 cut(s) 1854, 2356, 4140
FspBI CTAG 8 cut(s) 839, 1043, 2877, 3023, 3738, 3771, 3777, 4866
GlaI GCGC 2 cut(s) 1568, 3858
GsuI CTGGAG 3 cut(s) 987, 1885, 4280
HapII CCGG 4 cut(s) 986, 1433, 2027, 3272
HgaI GACGC 2 cut(s) 3200, 4806
HhaI GCGC 2 cut(s) 1569, 3859
Hin6I GCGC 2 cut(s) 1567, 3857
HinP1I GCGC 2 cut(s) 1567, 3857
HincII GTYRAC 4 cut(s) 886, 1663, 4323, 4694
HindII GTYRAC 4 cut(s) 886, 1663, 4323, 4694
HindIII AAGCTT 4 cut(s) 441, 1686, 3332, 4593
HpaI GTTAAC 1 cut(s) 1663
HpaII CCGG 4 cut(s) 986, 1433, 2027, 3272
HphI GGTGA 4 cut(s) 1112, 1598, 3473, 4336
Hpy99I CGWCG 1 cut(s) 935
HpyCH4IV ACGT 7 cut(s) 933, 1673, 2605, 3238, 3260, 3534, 3642
HpySE526I ACGT 7 cut(s) 933, 1673, 2605, 3238, 3260, 3534, 3642
HspAI GCGC 2 cut(s) 1567, 3857
Kpn2I TCCGGA 2 cut(s) 1432, 2026
KpnI GGTACC 1 cut(s) 780
Ksp22I TGATCA 3 cut(s) 222, 3958, 4420
KspAI GTTAAC 1 cut(s) 1663
KspI CCGCGG 1 cut(s) 3808
LguI GCTCTTC 2 cut(s) 1124, 2637
LmnI GCTCC 6 cut(s) 587, 903, 1866, 3015, 3853, 4261
MaeI CTAG 8 cut(s) 839, 1043, 2877, 3023, 3738, 3771, 3777, 4866
MaeII ACGT 7 cut(s) 933, 1673, 2605, 3238, 3260, 3534, 3642
MaeIII GTNAC 7 cut(s) 1799, 1931, 2774, 3076, 3256, 3987, 4202
MfeI CAATTG 2 cut(s) 3891, 4794
MflI RGATCY 9 cut(s) 337, 1074, 1153, 1555, 1678, 1942, 1960, 2335, 3314
MlsI TGGCCA 2 cut(s) 428, 3675
MluNI TGGCCA 2 cut(s) 428, 3675
MlyI GAGTC 5 cut(s) 714, 2050, 2512, 4144, 4160
MmeI TCCRAC 4 cut(s) 157, 1656, 3875, 4828
Mox20I TGGCCA 2 cut(s) 428, 3675
Mph1103I ATGCAT 2 cut(s) 1704, 4876
MroI TCCGGA 2 cut(s) 1432, 2026
MroXI GAANNNNTTC 3 cut(s) 273, 642, 1110
MscI TGGCCA 2 cut(s) 428, 3675
MslI CAYNNNNRTG 9 cut(s) 108, 1128, 1460, 1496, 1584, 3876, 3974, 3996, 4349
Msp20I TGGCCA 2 cut(s) 428, 3675
MspA1I CMGCKG 2 cut(s) 3807, 3964
MspCI CTTAAG 3 cut(s) 1708, 2678, 2960
MspI CCGG 4 cut(s) 986, 1433, 2027, 3272
MspR9I CCNGG 6 cut(s) 986, 987, 1560, 1864, 3273, 4349
MunI CAATTG 2 cut(s) 3891, 4794
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 3 cut(s) 1560, 1864, 4349
MvnI CGCG 3 cut(s) 1539, 3750, 3807
NciI CCSGG 3 cut(s) 986, 987, 3273
NcoI CCATGG 2 cut(s) 1938, 2378
NdeI CATATG 1 cut(s) 1493
NlaIV GGNNCC 8 cut(s) 778, 899, 1680, 2353, 2354, 2914, 3512, 4713
NmuCI GTSAC 2 cut(s) 3076, 4202
NotI GCGGCCGC 1 cut(s) 3808
NsiI ATGCAT 2 cut(s) 1704, 4876
NspI RCATGY 5 cut(s) 350, 1371, 3228, 3875, 4198
NspV TTCGAA 2 cut(s) 735, 3359
OliI CACNNNNGTG 2 cut(s) 1584, 4349
PaeI GCATGC 1 cut(s) 4198
PaeR7I CTCGAG 2 cut(s) 1011, 2750
PagI TCATGA 1 cut(s) 3972
PaqCI CACCTGC 1 cut(s) 1159
PciI ACATGT 1 cut(s) 346
PciSI GCTCTTC 2 cut(s) 1124, 2637
PcsI WCGNNNNNNNCGW 1 cut(s) 2366
PctI GAATGC 1 cut(s) 293
PdmI GAANNNNTTC 3 cut(s) 273, 642, 1110
PflMI CCANNNNNTGG 2 cut(s) 1519, 4354
PfoI TCCNGGA 2 cut(s) 1558, 3271
PleI GAGTC 5 cut(s) 714, 2049, 2511, 4144, 4160
PmaCI CACGTG 1 cut(s) 3535
PmlI CACGTG 1 cut(s) 3535
PpsI GAGTC 5 cut(s) 714, 2049, 2511, 4144, 4160
Ppu21I YACGTR 1 cut(s) 3535
PscI ACATGT 1 cut(s) 346
PsiI TTATAA 2 cut(s) 372, 1004
Psp124BI GAGCTC 2 cut(s) 1854, 4140
Psp1406I AACGTT 3 cut(s) 1673, 3238, 3260
Psp6I CCWGG 3 cut(s) 1558, 1862, 4347
PspCI CACGTG 1 cut(s) 3535
PspGI CCWGG 3 cut(s) 1558, 1862, 4347
PspN4I GGNNCC 8 cut(s) 778, 899, 1680, 2353, 2354, 2914, 3512, 4713
PspOMI GGGCCC 1 cut(s) 2352
PspPI GGNCC 7 cut(s) 179, 983, 2352, 2353, 3511, 3966, 4678
PstI CTGCAG 2 cut(s) 1189, 4759
PstNI CAGNNNCTG 6 cut(s) 1133, 1258, 3956, 4354, 4392, 4730
PsuI RGATCY 9 cut(s) 337, 1074, 1153, 1555, 1678, 1942, 1960, 2335, 3314
RseI CAYNNNNRTG 9 cut(s) 108, 1128, 1460, 1496, 1584, 3876, 3974, 3996, 4349
SacI GAGCTC 2 cut(s) 1854, 4140
SacII CCGCGG 1 cut(s) 3808
SapI GCTCTTC 2 cut(s) 1124, 2637
Sau96I GGNCC 7 cut(s) 179, 983, 2352, 2353, 3511, 3966, 4678
ScaI AGTACT 4 cut(s) 1549, 3775, 3787, 3912
SchI GAGTC 5 cut(s) 714, 2050, 2512, 4144, 4160
ScrFI CCNGG 6 cut(s) 986, 987, 1560, 1864, 3273, 4349
SfcI CTRYAG 7 cut(s) 657, 873, 1185, 2563, 3469, 4562, 4755
Sfr274I CTCGAG 2 cut(s) 1011, 2750
Sfr303I CCGCGG 1 cut(s) 3808
SfuI TTCGAA 2 cut(s) 735, 3359
SgrBI CCGCGG 1 cut(s) 3808
SinI GGWCC 2 cut(s) 179, 4678
SlaI CTCGAG 2 cut(s) 1011, 2750
SmaI CCCGGG 1 cut(s) 987
SmiMI CAYNNNNRTG 9 cut(s) 108, 1128, 1460, 1496, 1584, 3876, 3974, 3996, 4349
SpeI ACTAGT 3 cut(s) 1042, 3770, 3776
SphI GCATGC 1 cut(s) 4198
SspI AATATT 3 cut(s) 1814, 2119, 3328
SspMI CTAG 8 cut(s) 839, 1043, 2877, 3023, 3738, 3771, 3777, 4866
SstI GAGCTC 2 cut(s) 1854, 4140
StyD4I CCNGG 6 cut(s) 984, 985, 1558, 1862, 3271, 4347
StyI CCWWGG 3 cut(s) 1938, 2378, 3676
TaiI ACGT 7 cut(s) 936, 1676, 2608, 3241, 3263, 3537, 3645
TaqII GACCGA 1 cut(s) 377
TatI WGTACW 7 cut(s) 1547, 3773, 3785, 3910, 3937, 4330, 4493
TauI GCSGC 5 cut(s) 2992, 3753, 3810, 3813, 4283
TseFI GTSAC 2 cut(s) 3076, 4202
Tsp45I GTSAC 2 cut(s) 3076, 4202
TspGWI ACGGA 6 cut(s) 208, 431, 1711, 2157, 3200, 3323
TspMI CCCGGG 1 cut(s) 985
Van91I CCANNNNNTGG 2 cut(s) 1519, 4354
Vha464I CTTAAG 3 cut(s) 1708, 2678, 2960
VneI GTGCAC 1 cut(s) 3046
VpaK11BI GGWCC 2 cut(s) 179, 4678
XagI CCTNNNNNAGG 1 cut(s) 3758
XbaI TCTAGA 1 cut(s) 3022
XceI RCATGY 5 cut(s) 350, 1371, 3228, 3875, 4198
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XhoI CTCGAG 2 cut(s) 1011, 2750
XmaI CCCGGG 1 cut(s) 985
XmiI GTMKAC 1 cut(s) 4235
XmnI GAANNNNTTC 3 cut(s) 273, 642, 1110
XspI CTAG 8 cut(s) 839, 1043, 2877, 3023, 3738, 3771, 3777, 4866
ZrmI AGTACT 4 cut(s) 1549, 3775, 3787, 3912
Zsp2I ATGCAT 2 cut(s) 1704, 4876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.