MD12G1196900.v1.1

Transmembrane protein 184A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009384
Physical Location & Seq
Forward (+)
27796749 .. 27800877
4129 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1196900.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGGCGGAATCAACCCCAATATACTTGAGCATTATAGCTTTTTTCTGTACTGTTGGAGCCATGGCTTTGGCCATCATGCATATCTACAGGCACCTCATCAATTACACTGAACCCACTTATCAGAGATACATTGTCCGCATCATCTTCATGGTTCCGATATATGCATTGATGTCGTTCTTATCCCTTGTCATTCCTGCTGGTTCAATCTACTTTAATTCCATTAGAGAAGCCTATGAAGCTTGGGTCATTTATAATTTTTTATCGTTGTGCTTGGCATGGGTTGGTGGTCCAGGATCTGTGGTTCTAAGTTTAAGCGGTAGGGTTCTAAAGCCTTCCGTGGTTCTGATGACATGTTGCCTCCCTCCCATACCCCTGGATGGGCGCTTTATACGTAGGTGCAAACAAGGATGTTTGCAGTTTGTGATCCTGAAGCCTGTATTAGCTGTTGTTACACTCATACTTTACGCAAAGGGGAAATATGCAGATGGAAATTTCAGTCCAAGACAGTCATACCTGTATCTCACTATTATCTACACAATCTCATACACAACAGCTCTCTATGCTTTGGCGTTGTTTTACTTTGCTTGCAGAGATCTACTCCAGCCTTTCAATCCAGTTCCGAAGTTTATTATAATCAAATCTGTTGTCTTCCTCACTTATTGGCAGGGTGTGTTGGTCTTCCTTGCTGCGAAATCTGGCTTGATAAAGAATGCAGAGGATGCAGCTCAGTATCAAAATTTCATAATTTGTGTTGAGATGCTTATCGCTGCTATTGGTCATTTATATGCATTTCCTTACAAGGAGTATGCTGGTGCAAATATTGGCGGGCCTCGAAATCTGACTGGAAGCCTATCACATGCCGTAAAGTTGAATGACTTTTACCATGATACGGTCCACCAGTTTGCCCCTACTTATCATGATTATGTACTGTACAACCCTAGTGAGGGTGACGAGGGAAAGAGGAAATATCGGTCACGCACCTTTGTTCCGACTGGCCCAGAGATGGATGCTGTCAGAAGAAACAAGAACGTGTTTGGGAACAAGATAGATGACATTCAGCTTTCCAGTCTATCATCTTCTAGTGCAAGCACTCCAAATAATACTGGCCCAATACCTGAGTCTAAGAATTCAGATGCAATGAAGTCTTCCCTTCTTGTTGATACCTCGAATTCTGCATCAGTGCCGTATGACATGTCACTCATCGACTTAGACCTGAACAGTTACCCCGCAAAGGTTCCTTCAGCAAATGAAGGTGGCACTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Solute_trans_a

Protein Analysis

422

Amino Acids

46.84

Weight (kDa)

8.81

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 10 - 269 3.5e-92 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 252, 632
AccB1I GGYRCC 1 cut(s) 90
AciI CCGC 5 cut(s) 5, 136, 315, 825, 1227
AclWI GGATC 2 cut(s) 301, 418
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 4 cut(s) 490, 736, 1126, 1168
AcuI CTGAAG 2 cut(s) 449, 1224
AdeI CACNNNGTG 1 cut(s) 1263
AfaI GTAC 3 cut(s) 49, 927, 932
AfiI CCNNNNNNNGG 7 cut(s) 377, 378, 889, 943, 944, 1003, 1231
AflIII ACRYGT 3 cut(s) 350, 1029, 1191
AgsI TTSAA 3 cut(s) 204, 610, 871
AjnI CCWGG 2 cut(s) 289, 372
AloI GAACNNNNNNTCC 2 cut(s) 285, 317
AluBI AGCT 6 cut(s) 38, 239, 443, 554, 725, 1060
AluI AGCT 6 cut(s) 38, 239, 443, 554, 725, 1060
AlwI GGATC 2 cut(s) 301, 418
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 4 cut(s) 69, 827, 994, 1105
ApeKI GCWGC 3 cut(s) 686, 722, 767
ApoI RAATTY 4 cut(s) 490, 736, 1126, 1168
ArsI GACNNNNNNTTYG 2 cut(s) 630, 662
AspLEI GCGC 1 cut(s) 384
AspS9I GGNCC 5 cut(s) 287, 827, 892, 995, 1106
AsuHPI GGTGA 1 cut(s) 959
AvaII GGWCC 2 cut(s) 287, 892
BalI TGGCCA 1 cut(s) 71
BanI GGYRCC 1 cut(s) 90
BbsI GAAGAC 3 cut(s) 640, 670, 1137
BbvI GCAGC 3 cut(s) 673, 734, 754
BccI CCATC 4 cut(s) 80, 371, 479, 997
BceAI ACGGC 2 cut(s) 845, 1168
BcgI CGANNNNNNTGC 2 cut(s) 153, 187
BciT130I CCWGG 2 cut(s) 291, 374
BfaI CTAG 3 cut(s) 939, 1080, 1260
BfmI CTRYAG 1 cut(s) 85
BfoI RGCGCY 1 cut(s) 385
BglII AGATCT 1 cut(s) 592
BisI GCNGC 3 cut(s) 687, 723, 768
BlsI GCNGC 3 cut(s) 688, 724, 769
Bme1390I CCNGG 2 cut(s) 291, 374
Bme18I GGWCC 2 cut(s) 287, 892
BmgT120I GGNCC 5 cut(s) 287, 827, 892, 995, 1106
BmiI GGNNCC 4 cut(s) 58, 92, 153, 1236
BmrFI CCNGG 2 cut(s) 291, 374
BmsI GCATC 6 cut(s) 147, 709, 747, 997, 1123, 1184
BpiI GAAGAC 3 cut(s) 640, 670, 1137
BplI GAGNNNNNCTC 2 cut(s) 582, 614
BpmI CTGGAG 1 cut(s) 584
BpuEI CTTGAG 1 cut(s) 46
BsaAI YACGTR 1 cut(s) 392
BsaBI GATNNNNATC 1 cut(s) 761
BsaJI CCNNGG 3 cut(s) 60, 336, 372
Bsc4I CCNNNNNNNGG 7 cut(s) 377, 378, 889, 943, 944, 1003, 1231
Bse1I ACTGG 6 cut(s) 614, 847, 898, 997, 1065, 1108
Bse3DI GCAATG 1 cut(s) 1143
Bse8I GATNNNNATC 1 cut(s) 761
BseBI CCWGG 2 cut(s) 291, 374
BseDI CCNNGG 3 cut(s) 60, 336, 372
BseGI GGATG 4 cut(s) 382, 413, 724, 1012
BseJI GATNNNNATC 1 cut(s) 761
BseLI CCNNNNNNNGG 7 cut(s) 377, 378, 889, 943, 944, 1003, 1231
BseMI GCAATG 1 cut(s) 1143
BseMII CTCAG 2 cut(s) 740, 1107
BseNI ACTGG 6 cut(s) 614, 847, 898, 997, 1065, 1108
BseXI GCAGC 3 cut(s) 673, 734, 754
BshFI GGCC 4 cut(s) 71, 829, 996, 1107
BshNI GGYRCC 1 cut(s) 90
BslI CCNNNNNNNGG 7 cut(s) 377, 378, 889, 943, 944, 1003, 1231
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 4 cut(s) 71, 829, 996, 1107
Bsp1407I TGTACA 1 cut(s) 930
Bsp143I GATC 3 cut(s) 293, 423, 592
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 5 cut(s) 5, 136, 315, 825, 1227
BspANI GGCC 4 cut(s) 71, 829, 996, 1107
BspCNI CTCAG 2 cut(s) 739, 1108
BspHI TCATGA 1 cut(s) 916
BspLI GGNNCC 4 cut(s) 58, 92, 153, 1236
BspPI GGATC 2 cut(s) 301, 418
BspT107I GGYRCC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 1143
BsrGI TGTACA 1 cut(s) 930
BsrI ACTGG 6 cut(s) 614, 847, 898, 997, 1065, 1108
BssECI CCNNGG 3 cut(s) 60, 336, 372
BssMI GATC 3 cut(s) 293, 423, 592
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 291, 374
Bst4CI ACNGT 5 cut(s) 52, 507, 892, 930, 1220
BstAPI GCANNNNNTGC 1 cut(s) 719
BstAUI TGTACA 1 cut(s) 930
BstBAI YACGTR 1 cut(s) 392
BstC8I GCNNGC 3 cut(s) 586, 827, 1087
BstDEI CTNAG 5 cut(s) 305, 726, 1116, 1122, 1207
BstDSI CCRYGG 2 cut(s) 60, 336
BstF5I GGATG 4 cut(s) 382, 413, 724, 1012
BstH2I RGCGCY 1 cut(s) 385
BstHHI GCGC 1 cut(s) 384
BstKTI GATC 3 cut(s) 296, 426, 595
BstMBI GATC 3 cut(s) 293, 423, 592
BstMWI GCNNNNNNNGC 3 cut(s) 236, 560, 719
BstNI CCWGG 2 cut(s) 291, 374
BstNSI RCATGY 3 cut(s) 354, 860, 1195
BstSCI CCNGG 2 cut(s) 289, 372
BstSFI CTRYAG 1 cut(s) 85
BstSNI TACGTA 1 cut(s) 392
BstV1I GCAGC 3 cut(s) 673, 734, 754
BstV2I GAAGAC 3 cut(s) 640, 670, 1137
BstX2I RGATCY 2 cut(s) 293, 592
BstXI CCANNNNNNTGG 2 cut(s) 67, 373
BstYI RGATCY 2 cut(s) 293, 592
BsuRI GGCC 4 cut(s) 71, 829, 996, 1107
BtgI CCRYGG 2 cut(s) 60, 336
BtsCI GGATG 4 cut(s) 382, 413, 724, 1012
BtsIMutI CAGTG 2 cut(s) 105, 1185
Cac8I GCNNGC 3 cut(s) 586, 827, 1087
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 1 cut(s) 916
CfoI GCGC 1 cut(s) 384
Cfr13I GGNCC 5 cut(s) 287, 827, 892, 995, 1106
Csp6I GTAC 3 cut(s) 48, 926, 931
CviAII CATG 9 cut(s) 61, 76, 148, 276, 351, 857, 884, 917, 1192
CviQI GTAC 3 cut(s) 48, 926, 931
DdeI CTNAG 5 cut(s) 305, 726, 1116, 1122, 1207
DpnI GATC 3 cut(s) 295, 425, 594
DpnII GATC 3 cut(s) 293, 423, 592
DraIII CACNNNGTG 1 cut(s) 1263
EaeI YGGCCR 1 cut(s) 69
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 392
Eco130I CCWWGG 1 cut(s) 60
Eco47I GGWCC 2 cut(s) 287, 892
Eco57I CTGAAG 2 cut(s) 449, 1224
EcoRI GAATTC 2 cut(s) 1126, 1168
EcoRII CCWGG 2 cut(s) 289, 372
EcoT14I CCWWGG 1 cut(s) 60
EcoT22I ATGCAT 3 cut(s) 81, 166, 790
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 9 cut(s) 64, 79, 151, 279, 354, 860, 887, 920, 1195
FatI CATG 9 cut(s) 60, 75, 147, 275, 350, 856, 883, 916, 1191
FauI CCCGC 2 cut(s) 818, 1234
Fnu4HI GCNGC 3 cut(s) 687, 723, 768
FokI GGATG 4 cut(s) 389, 420, 731, 1019
Fsp4HI GCNGC 3 cut(s) 687, 723, 768
FspBI CTAG 3 cut(s) 939, 1080, 1260
GlaI GCGC 1 cut(s) 383
GluI GCNGC 3 cut(s) 687, 723, 768
GsuI CTGGAG 1 cut(s) 584
HaeII RGCGCY 1 cut(s) 385
HaeIII GGCC 4 cut(s) 71, 829, 996, 1107
HhaI GCGC 1 cut(s) 384
Hin1II CATG 9 cut(s) 64, 79, 151, 279, 354, 860, 887, 920, 1195
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 2 cut(s) 8, 1118
HphI GGTGA 1 cut(s) 959
Hpy166II GTNNAC 1 cut(s) 895
Hpy188I TCNGA 8 cut(s) 123, 156, 345, 621, 840, 990, 1016, 1132
Hpy188III TCNNGA 2 cut(s) 427, 917
Hpy8I GTNNAC 1 cut(s) 895
HpyAV CCTTC 4 cut(s) 342, 1160, 1244, 1248
HpyCH4III ACNGT 5 cut(s) 52, 507, 892, 930, 1220
HpyCH4IV ACGT 2 cut(s) 391, 1029
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 560, 719
HpyF3I CTNAG 5 cut(s) 305, 726, 1116, 1122, 1207
HpySE526I ACGT 2 cut(s) 391, 1029
Hsp92II CATG 9 cut(s) 64, 79, 151, 279, 354, 860, 887, 920, 1195
HspAI GCGC 1 cut(s) 382
Kzo9I GATC 3 cut(s) 293, 423, 592
LmnI GCTCC 1 cut(s) 56
Lsp1109I GCAGC 3 cut(s) 673, 734, 754
LweI GCATC 6 cut(s) 147, 709, 747, 997, 1123, 1184
MaeI CTAG 3 cut(s) 939, 1080, 1260
MaeII ACGT 2 cut(s) 391, 1029
MaeIII GTNAC 5 cut(s) 448, 947, 972, 1194, 1220
MalI GATC 3 cut(s) 295, 425, 594
MboI GATC 3 cut(s) 293, 423, 592
MboII GAAGA 6 cut(s) 136, 640, 670, 1029, 1068, 1137
MflI RGATCY 2 cut(s) 293, 592
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 8 cut(s) 100, 214, 253, 490, 736, 744, 1126, 1168
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 1 cut(s) 1127
MmeI TCCRAC 2 cut(s) 34, 1013
Mox20I TGGCCA 1 cut(s) 71
Mph1103I ATGCAT 3 cut(s) 81, 166, 790
MscI TGGCCA 1 cut(s) 71
MseI TTAA 2 cut(s) 213, 311
MslI CAYNNNNRTG 3 cut(s) 146, 783, 921
Msp20I TGGCCA 1 cut(s) 71
MspR9I CCNGG 2 cut(s) 291, 374
Mva1269I GAATGC 1 cut(s) 715
MvaI CCWGG 2 cut(s) 291, 374
MwoI GCNNNNNNNGC 3 cut(s) 236, 560, 719
NcoI CCATGG 1 cut(s) 60
NdeII GATC 3 cut(s) 293, 423, 592
NlaIII CATG 9 cut(s) 64, 79, 151, 279, 354, 860, 887, 920, 1195
NlaIV GGNNCC 4 cut(s) 58, 92, 153, 1236
NmuCI GTSAC 3 cut(s) 947, 972, 1194
NsiI ATGCAT 3 cut(s) 81, 166, 790
NspI RCATGY 3 cut(s) 354, 860, 1195
PagI TCATGA 1 cut(s) 916
PciI ACATGT 2 cut(s) 350, 1191
PctI GAATGC 1 cut(s) 715
PfeI GAWTC 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 289
PkrI GCNGC 3 cut(s) 688, 724, 769
PleI GAGTC 1 cut(s) 1126
PpsI GAGTC 1 cut(s) 1126
Ppu21I YACGTR 1 cut(s) 392
PscI ACATGT 2 cut(s) 350, 1191
PsiI TTATAA 2 cut(s) 252, 632
Psp6I CCWGG 2 cut(s) 289, 372
PspGI CCWGG 2 cut(s) 289, 372
PspN4I GGNNCC 4 cut(s) 58, 92, 153, 1236
PspPI GGNCC 5 cut(s) 287, 827, 892, 995, 1106
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 2 cut(s) 293, 592
RsaI GTAC 3 cut(s) 49, 927, 932
RsaNI GTAC 3 cut(s) 48, 926, 931
RseI CAYNNNNRTG 3 cut(s) 146, 783, 921
SaqAI TTAA 2 cut(s) 213, 311
SatI GCNGC 3 cut(s) 687, 723, 768
Sau3AI GATC 3 cut(s) 293, 423, 592
Sau96I GGNCC 5 cut(s) 287, 827, 892, 995, 1106
SchI GAGTC 1 cut(s) 1127
ScrFI CCNGG 2 cut(s) 291, 374
SfaNI GCATC 6 cut(s) 147, 709, 747, 997, 1123, 1184
SfcI CTRYAG 1 cut(s) 85
SinI GGWCC 2 cut(s) 287, 892
SmiMI CAYNNNNRTG 3 cut(s) 146, 783, 921
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
SnaBI TACGTA 1 cut(s) 392
Sse9I AATT 8 cut(s) 100, 214, 253, 490, 736, 744, 1126, 1168
SsiI CCGC 5 cut(s) 5, 136, 315, 825, 1227
SspI AATATT 1 cut(s) 820
SspMI CTAG 3 cut(s) 939, 1080, 1260
StyD4I CCNGG 2 cut(s) 289, 372
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 5 cut(s) 52, 507, 892, 930, 1220
TaiI ACGT 2 cut(s) 394, 1032
TaqI TCGA 3 cut(s) 832, 1166, 1203
TaqII GACCGA 1 cut(s) 960
TasI AATT 8 cut(s) 100, 214, 253, 490, 736, 744, 1126, 1168
TatI WGTACW 3 cut(s) 47, 925, 930
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 2 cut(s) 213, 311
Tru9I TTAA 2 cut(s) 213, 311
TscAI CASTG 2 cut(s) 112, 1185
TseFI GTSAC 3 cut(s) 947, 972, 1194
TseI GCWGC 3 cut(s) 686, 722, 767
Tsp45I GTSAC 3 cut(s) 947, 972, 1194
TspDTI ATGAA 5 cut(s) 136, 249, 730, 1154, 1263
TspGWI ACGGA 1 cut(s) 325
TspRI CASTG 2 cut(s) 112, 1185
VpaK11BI GGWCC 2 cut(s) 287, 892
XapI RAATTY 4 cut(s) 490, 736, 1126, 1168
XceI RCATGY 3 cut(s) 354, 860, 1195
XspI CTAG 3 cut(s) 939, 1080, 1260
Zsp2I ATGCAT 3 cut(s) 81, 166, 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.