Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004855

Physical Location & Seq

Genomic Coordinates Forward (+)

27539356 .. 27541647

Gene Structure 2292 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1184500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGCCAAGCCAACCTACAGCTCCCTCGAGCCGGTGGACAAGAAGAAGGGAGGGCTCACTGCCTCCAGCTTCATTTTCGGTAAGTAGCCATTCTCAGAAAAAGGGTGTGCGGAAATCCCTTCTGTGCAGAATTCTATCTGTGTTGCTGGCGTTGGACAGCATGGGATTCGTCGCGAATATGGTCAGCATGGTGCTGTACTTTATGTACGTGATGCATTTTGATCTGGCGCACTCCTCCAACCTTCTCACAAACTTCATGGGATCAACTTTCCTCCTCTCCCTCGTTGGAGGCTTCATATCGGACACATACTTGAGCAGATTCACAACATGCCTGATTTTTGGCGTAATTGAGATACTGGCTTTGGTGATGATGACAACGCAAGCTTATTCAAAGCATTTGCACCCAACAAAAACTTGCCTTTCAAATTGTGTCCAAGGCCGTATCGCCGCAATGCTATATGGATCCCTGTGCTTGTTGGCACTGGGAACAGGTGGCGTGAGGGGAGCGCTGCCGGCACTTGGTGCTGACCAGTTTAACCGGAAAGAGGAAGCAAAGTTGCTGGCGACGTTTTTCAATTGGTTTATGCTCAGTACTACAGCTGGAGCAACTATTGGTGTCACGGCCATTGTGTATGTGAGCATGAATGTAGATTGGTACTGGGGTTTCTTTATATCAACTATTGCAGCCGTTATTGGGTTCTCTGTTCTTGCAATGGGTAAGCCTTTCTATCGCCTCCAAGTCCCCGGTGACAGCCCCATCATAAGGATCGTTCAGGTTATTGTCGTTGCGATAAAAAATAGGCGTTTACCGCAGCCAGAGAGCTCGCATGAACTGTATGAGATTAGTGAGAAAGAATCAAATTATACGGAAGAAAAAATTACCCATACACATCAATTCAGTTGTCTGGACAAAGCAGCCATACTTGGCAAAGGCTCCAAGCCCGAACCATGGAAGGTTTGCACAGTAACACAAGTGGAAGAAGTGAAGGTCCTGACAAGAATGTTGCCAATTCTCTTCAGCACCATCATAATGAACACTTGTTTGGCACAACTCCAAACATTCTCAGTCCAACAAGGAAGCATCATGGACCTCCACTTGGGCTCCATCAAGGTTCCGGCGCCATCTATCCCCGTCATTCCTCTCCTCTTCATGTCCATCCTCATCCCACTGTACGAGTACTTCTTCGTGCCTTTCGCGAGAAAGATCACAAAGCACCCAGCAGGCATAACGCAGCTTCAGCGCGTTGGTGTTGGACTAGTCCTCTCCGCAATCTCAATGGCGGTAGCGGGCCTGGTGGAGGTGAAAAGAAAGAACCAAGCCAACAAGGACTTCACAAAGCCGATATCTCTCTTCTGGCTTTCTTTCCAATACGCTATTTTTGGTATTGCTGACATGTTTACCCTAGTAGGGTTACTTGAATTCTTCTACAAGGAAGCTCCAGCAGGGATGAAATCGCTTTCTACTTCATTCACTTTTCTTTCACTTTCTTTTGGGTACTTCTTGAGCTCAGTTTTCGTTGACTTGATCAATGCCGTGACGAAAAGAGTTGCTCCGAGCAAACAAGGATGGCTCTATGGAAAAGACCTAAACAAAAACAATTTGCAGCTATTTTACTATTTCTTGGCAATCCTTAGCTGCATCAACTTTGTGAACTATGTATATTGGGCTTCATGGTACAAGTACAAAGCAGATGAGACAGATCTAAAGCTGCAGCTTAAAACTTTGCACCAAACACCTCTCATGCACAATGATACAAGTGTTGTTCAAGAAAGTGGAAGCAAGATTGATGAAGCTGCTACCTCCACTGAGGAAAATAAAGAAAAGGTGGCAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005319 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0008028 GO:0008150 GO:0008509 GO:0008514 GO:0009605 GO:0009607 GO:0009624 GO:0010119 GO:0010876 GO:0015075 GO:0015318 GO:0015665 GO:0015711 GO:0015718 GO:0015849 GO:0015850 GO:0016020 GO:0022857 GO:0033036 GO:0034220 GO:0043207 GO:0044464 GO:0046864 GO:0046865 GO:0046942 GO:0046943 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0065007 GO:0071702 GO:0071944 GO:0080168 GO:0090440 GO:0098656 GO:1901618 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PTR2

Protein Analysis

612

Amino Acids

68.07

Weight (kDa)

9.23

Isoelectric Point (pI)

39.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	46 - 502	1.4e-11	Major Facilitator Superfamily
PTR2	PF00854	107 - 521	3.4e-79	POT family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004855)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g21811 FvH4_4g21812 FvH4_6g08650 FvH4_6g08650
malus_domestica	MD04G1184500.v1.1 MD12G1197600.v1.1 MD12G1197700.v1.1 MD13G1136700.v1.1 MD16G1132000.v1.1
prunus_persica	Prupe.1G208700_v2.0.a1 Prupe.6G303000_v2.0.a1
pyrus_communis	pycom04g16360
rosa_chinensis	RchiOBHm_Chr3g0458781 RchiOBHm_Chr4g0428051
rosa_laevigata	RLG00000007186 RLG00000025135
rosa_multiflora	Rmu_co8474865.1_g000001 Rmu_sc0001634.1_g000029
rosa_roxburghii	Rroxscaffold_5G00369770 Rroxscaffold_6G00420790
rosa_rugosa	Rorug03G0031800
rosa_samantha	Rh3AG089500 Rh3BG092400 Rh3CG092700 Rh3DG093400 Rh4AG279100 Rh4BG284500 Rh4CG300200 Rh4DG281600
rosa_wichuraiana	Rw3G007660 Rw4G024250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1117
AccII	CGCG	3 cut(s)	173, 1196, 1242
AciI	CCGC	6 cut(s)	109, 447, 809, 1266, 1280, 1286
AclWI	GGATC	4 cut(s)	268, 456, 469, 773
AcoI	YGGCCR	1 cut(s)	621
AcsI	RAATTY	2 cut(s)	129, 1418
AcuI	CTGAAG	2 cut(s)	1000, 1220
AcyI	GRCGYC	1 cut(s)	1118
AdeI	CACNNNGTG	1 cut(s)	521
AfaI	GTAC	9 cut(s)	197, 206, 592, 656, 1172, 1178, 1496, 1676, 1682
AfeI	AGCGCT	1 cut(s)	507
AfiI	CCNNNNNNNGG	9 cut(s)	30, 518, 544, 693, 762, 948, 1096, 1297, 1407
AflIII	ACRYGT	1 cut(s)	1392
AgsI	TTSAA	5 cut(s)	390, 423, 574, 1418, 1766
AhlI	ACTAGT	1 cut(s)	1255
AjnI	CCWGG	1 cut(s)	1290
AjuI	GAANNNNNNNTTGG	4 cut(s)	1025, 1057, 1313, 1345
Alw21I	GWGCWC	2 cut(s)	824, 1508
Alw26I	GTCTC	1 cut(s)	1688
AlwI	GGATC	4 cut(s)	268, 456, 469, 773
Ama87I	CYCGRG	1 cut(s)	25
Aor51HI	AGCGCT	1 cut(s)	507
AoxI	GGCC	3 cut(s)	436, 621, 1288
ApoI	RAATTY	2 cut(s)	129, 1418
AspLEI	GCGC	4 cut(s)	229, 508, 1120, 1242
AspS9I	GGNCC	3 cut(s)	988, 1087, 1288
AsuC2I	CCSGG	1 cut(s)	744
AsuHPI	GGTGA	3 cut(s)	376, 758, 1312
AvaI	CYCGRG	1 cut(s)	25
AvaII	GGWCC	2 cut(s)	988, 1087
BaeI	ACNNNNGTAYC	4 cut(s)	646, 679, 1743, 1776
BamHI	GGATCC	1 cut(s)	461
BanI	GGYRCC	1 cut(s)	1117
BanII	GRGCYC	4 cut(s)	56, 824, 1103, 1508
BarI	GAAGNNNNNNTAC	2 cut(s)	1163, 1195
Bbv12I	GWGCWC	2 cut(s)	824, 1508
BccI	CCATC	6 cut(s)	764, 1031, 1112, 1129, 1163, 1560
BceAI	ACGGC	4 cut(s)	423, 636, 671, 1517
BcgI	CGANNNNNNTGC	2 cut(s)	148, 182
BciT130I	CCWGG	1 cut(s)	1292
BclI	TGATCA	1 cut(s)	1524
BcnI	CCSGG	1 cut(s)	744
BcoDI	GTCTC	1 cut(s)	1688
BcuI	ACTAGT	1 cut(s)	1255
BfaI	CTAG	2 cut(s)	1256, 1403
BfmI	CTRYAG	3 cut(s)	15, 594, 1709
BfoI	RGCGCY	2 cut(s)	509, 1121
BglII	AGATCT	1 cut(s)	1699
BmcAI	AGTACT	2 cut(s)	592, 1178
Bme1390I	CCNGG	2 cut(s)	744, 1292
Bme18I	GGWCC	2 cut(s)	988, 1087
BmeT110I	CYCGRG	1 cut(s)	25
BmgT120I	GGNCC	3 cut(s)	988, 1087, 1288
BmiI	GGNNCC	5 cut(s)	463, 934, 1102, 1113, 1119
BmrFI	CCNGG	2 cut(s)	744, 1292
BmrI	ACTGGG	2 cut(s)	491, 667
BmsI	GCATC	3 cut(s)	201, 1089, 1647
BmuI	ACTGGG	2 cut(s)	491, 667
BpmI	CTGGAG	3 cut(s)	48, 621, 1422
Bpu10I	CCTNAGC	1 cut(s)	1631
BpuEI	CTTGAG	2 cut(s)	331, 1522
BpuMI	CCSGG	1 cut(s)	744
BsaAI	YACGTR	1 cut(s)	208
BsaHI	GRCGYC	1 cut(s)	1118
BsaJI	CCNNGG	3 cut(s)	433, 742, 947
BsaWI	WCCGGW	1 cut(s)	537
BsaXI	ACNNNNNCTCC	2 cut(s)	1085, 1115
Bsc4I	CCNNNNNNNGG	9 cut(s)	30, 518, 544, 693, 762, 948, 1096, 1297, 1407
Bse118I	RCCGGY	2 cut(s)	30, 511
Bse1I	ACTGG	4 cut(s)	360, 486, 529, 662
Bse3DI	GCAATG	2 cut(s)	456, 717
BseBI	CCWGG	1 cut(s)	1292
BseDI	CCNNGG	3 cut(s)	433, 742, 947
BseGI	GGATG	4 cut(s)	1155, 1161, 1452, 1571
BseLI	CCNNNNNNNGG	9 cut(s)	30, 518, 544, 693, 762, 948, 1096, 1297, 1407
BseMI	GCAATG	2 cut(s)	456, 717
BseMII	CTCAG	5 cut(s)	107, 601, 1077, 1521, 1797
BseNI	ACTGG	4 cut(s)	360, 486, 529, 662
BseRI	GAGGAG	3 cut(s)	223, 263, 1133
BseYI	CCCAGC	1 cut(s)	1216
BsgI	GTGCAG	1 cut(s)	145
Bsh1236I	CGCG	3 cut(s)	173, 1196, 1242
BshFI	GGCC	3 cut(s)	438, 623, 1290
BshNI	GGYRCC	1 cut(s)	1117
BsiHKAI	GWGCWC	2 cut(s)	824, 1508
BsiHKCI	CYCGRG	1 cut(s)	25
BsiSI	CCGG	5 cut(s)	31, 512, 538, 744, 1115
BslFI	GGGAC	1 cut(s)	725
BslI	CCNNNNNNNGG	9 cut(s)	30, 518, 544, 693, 762, 948, 1096, 1297, 1407
BsmAI	GTCTC	1 cut(s)	1688
BsmFI	GGGAC	1 cut(s)	725
BsnI	GGCC	3 cut(s)	438, 623, 1290
BsoBI	CYCGRG	1 cut(s)	25
Bsp1286I	GDGCHC	4 cut(s)	56, 824, 1103, 1508
Bsp143I	GATC	7 cut(s)	220, 260, 461, 765, 1203, 1524, 1699
Bsp19I	CCATGG	1 cut(s)	947
Bsp68I	TCGCGA	2 cut(s)	173, 1196
BspACI	CCGC	6 cut(s)	109, 447, 809, 1266, 1280, 1286
BspANI	GGCC	3 cut(s)	438, 623, 1290
BspCNI	CTCAG	5 cut(s)	106, 600, 1076, 1520, 1798
BspFNI	CGCG	3 cut(s)	173, 1196, 1242
BspLI	GGNNCC	5 cut(s)	463, 934, 1102, 1113, 1119
BspMAI	CTGCAG	1 cut(s)	1713
BspPI	GGATC	4 cut(s)	268, 456, 469, 773
BspT107I	GGYRCC	1 cut(s)	1117
BsrDI	GCAATG	2 cut(s)	456, 717
BsrFI	RCCGGY	2 cut(s)	30, 511
BsrI	ACTGG	4 cut(s)	360, 486, 529, 662
BssAI	RCCGGY	2 cut(s)	30, 511
BssECI	CCNNGG	3 cut(s)	433, 742, 947
BssMI	GATC	7 cut(s)	220, 260, 461, 765, 1203, 1524, 1699
BssNI	GRCGYC	1 cut(s)	1118
BssT1I	CCWWGG	2 cut(s)	433, 947
Bst2UI	CCWGG	1 cut(s)	1292
Bst4CI	ACNGT	3 cut(s)	834, 964, 1170
Bst6I	CTCTTC	3 cut(s)	1019, 1151, 1355
BstACI	GRCGYC	1 cut(s)	1118
BstAPI	GCANNNNNTGC	1 cut(s)	521
BstBAI	YACGTR	1 cut(s)	208
BstC8I	GCNNGC	7 cut(s)	147, 381, 513, 561, 824, 1222, 1288
BstDEI	CTNAG	6 cut(s)	93, 587, 1063, 1507, 1631, 1806
BstDSI	CCRYGG	1 cut(s)	947
BstENI	CCTNNNNNAGG	1 cut(s)	1295
BstF5I	GGATG	4 cut(s)	1155, 1161, 1452, 1571
BstFNI	CGCG	3 cut(s)	173, 1196, 1242
BstH2I	RGCGCY	2 cut(s)	509, 1121
BstHHI	GCGC	4 cut(s)	229, 508, 1120, 1242
BstKTI	GATC	7 cut(s)	223, 263, 464, 768, 1206, 1527, 1702
BstMAI	GTCTC	1 cut(s)	1688
BstMBI	GATC	7 cut(s)	220, 260, 461, 765, 1203, 1524, 1699
BstMWI	GCNNNNNNNGC	4 cut(s)	512, 521, 808, 1237
BstNI	CCWGG	1 cut(s)	1292
BstNSI	RCATGY	2 cut(s)	330, 1396
BstSCI	CCNGG	2 cut(s)	742, 1290
BstSFI	CTRYAG	3 cut(s)	15, 594, 1709
BstUI	CGCG	3 cut(s)	173, 1196, 1242
BstX2I	RGATCY	2 cut(s)	461, 1699
BstYI	RGATCY	2 cut(s)	461, 1699
BsuRI	GGCC	3 cut(s)	438, 623, 1290
BtgI	CCRYGG	1 cut(s)	947
BtsCI	GGATG	4 cut(s)	1155, 1161, 1452, 1571
BtsI	GCAGTG	1 cut(s)	56
BtsIMutI	CAGTG	4 cut(s)	56, 479, 1166, 1803
BtuMI	TCGCGA	2 cut(s)	173, 1196
Cac8I	GCNNGC	7 cut(s)	147, 381, 513, 561, 824, 1222, 1288
CfoI	GCGC	4 cut(s)	229, 508, 1120, 1242
Cfr10I	RCCGGY	2 cut(s)	30, 511
Cfr13I	GGNCC	3 cut(s)	988, 1087, 1288
Csp6I	GTAC	9 cut(s)	196, 205, 591, 655, 1171, 1177, 1495, 1675, 1681
CviQI	GTAC	9 cut(s)	196, 205, 591, 655, 1171, 1177, 1495, 1675, 1681
DdeI	CTNAG	6 cut(s)	93, 587, 1063, 1507, 1631, 1806
DinI	GGCGCC	1 cut(s)	1119
DpnI	GATC	7 cut(s)	222, 262, 463, 767, 1205, 1526, 1701
DpnII	GATC	7 cut(s)	220, 260, 461, 765, 1203, 1524, 1699
DraIII	CACNNNGTG	1 cut(s)	521
EaeI	YGGCCR	1 cut(s)	621
Eam1104I	CTCTTC	3 cut(s)	1019, 1151, 1355
EarI	CTCTTC	3 cut(s)	1019, 1151, 1355
Ecl136II	GAGCTC	2 cut(s)	822, 1506
Eco130I	CCWWGG	2 cut(s)	433, 947
Eco24I	GRGCYC	4 cut(s)	56, 824, 1103, 1508
Eco32I	GATATC	1 cut(s)	1344
Eco47I	GGWCC	2 cut(s)	988, 1087
Eco47III	AGCGCT	1 cut(s)	507
Eco53kI	GAGCTC	2 cut(s)	822, 1506
Eco57I	CTGAAG	2 cut(s)	1000, 1220
Eco88I	CYCGRG	1 cut(s)	25
EcoICRI	GAGCTC	2 cut(s)	822, 1506
EcoNI	CCTNNNNNAGG	1 cut(s)	1295
EcoO109I	RGGNCCY	1 cut(s)	988
EcoRI	GAATTC	2 cut(s)	129, 1418
EcoRII	CCWGG	1 cut(s)	1290
EcoRV	GATATC	1 cut(s)	1344
EcoT14I	CCWWGG	2 cut(s)	433, 947
EcoT22I	ATGCAT	1 cut(s)	216
EcoT38I	GRGCYC	4 cut(s)	56, 824, 1103, 1508
EgeI	GGCGCC	1 cut(s)	1119
EheI	GGCGCC	1 cut(s)	1119
ErhI	CCWWGG	2 cut(s)	433, 947
FaqI	GGGAC	1 cut(s)	725
FauI	CCCGC	1 cut(s)	1279
FbaI	TGATCA	1 cut(s)	1524
FokI	GGATG	4 cut(s)	1142, 1148, 1459, 1578
FriOI	GRGCYC	4 cut(s)	56, 824, 1103, 1508
FspBI	CTAG	2 cut(s)	1256, 1403
GlaI	GCGC	4 cut(s)	228, 507, 1119, 1241
GsaI	CCCAGC	1 cut(s)	1220
GsuI	CTGGAG	3 cut(s)	48, 621, 1422
HaeII	RGCGCY	2 cut(s)	509, 1121
HaeIII	GGCC	3 cut(s)	438, 623, 1290
HapII	CCGG	5 cut(s)	31, 512, 538, 744, 1115
HhaI	GCGC	4 cut(s)	229, 508, 1120, 1242
Hin1I	GRCGYC	1 cut(s)	1118
Hin6I	GCGC	4 cut(s)	227, 506, 1118, 1240
HinP1I	GCGC	4 cut(s)	227, 506, 1118, 1240
HincII	GTYRAC	1 cut(s)	1519
HindII	GTYRAC	1 cut(s)	1519
HindIII	AAGCTT	1 cut(s)	381
HinfI	GANTC	3 cut(s)	165, 318, 854
HpaII	CCGG	5 cut(s)	31, 512, 538, 744, 1115
HphI	GGTGA	3 cut(s)	376, 758, 1312
Hpy166II	GTNNAC	5 cut(s)	36, 806, 1398, 1519, 1651
Hpy188I	TCNGA	3 cut(s)	96, 301, 1554
Hpy188III	TCNNGA	6 cut(s)	172, 905, 991, 1195, 1501, 1766
Hpy8I	GTNNAC	5 cut(s)	36, 806, 1398, 1519, 1651
Hpy99I	CGWCG	2 cut(s)	173, 568
HpyAV	CCTTC	5 cut(s)	39, 128, 251, 946, 979
HpyCH4III	ACNGT	3 cut(s)	834, 964, 1170
HpyCH4IV	ACGT	2 cut(s)	207, 566
HpyF10VI	GCNNNNNNNGC	4 cut(s)	512, 521, 808, 1237
HpyF3I	CTNAG	6 cut(s)	93, 587, 1063, 1507, 1631, 1806
HpySE526I	ACGT	2 cut(s)	207, 566
Hsp92I	GRCGYC	1 cut(s)	1118
HspAI	GCGC	4 cut(s)	227, 506, 1118, 1240
KasI	GGCGCC	1 cut(s)	1117
KroI	GCCGGC	1 cut(s)	511
KroNI	GCCGGC	1 cut(s)	513
Ksp22I	TGATCA	1 cut(s)	1524
Kzo9I	GATC	7 cut(s)	220, 260, 461, 765, 1203, 1524, 1699
LmnI	GCTCC	7 cut(s)	25, 503, 602, 938, 1106, 1441, 1555
LweI	GCATC	3 cut(s)	201, 1089, 1647
MaeI	CTAG	2 cut(s)	1256, 1403
MaeII	ACGT	2 cut(s)	207, 566
MaeIII	GTNAC	5 cut(s)	616, 746, 964, 1410, 1534
MalI	GATC	7 cut(s)	222, 262, 463, 767, 1205, 1526, 1701
MboI	GATC	7 cut(s)	220, 260, 461, 765, 1203, 1524, 1699
MboII	GAAGA	8 cut(s)	54, 881, 989, 1006, 1138, 1174, 1342, 1414
MfeI	CAATTG	1 cut(s)	574
MflI	RGATCY	2 cut(s)	461, 1699
MhlI	GDGCHC	4 cut(s)	56, 824, 1103, 1508
Mly113I	GGCGCC	1 cut(s)	1118
MmeI	TCCRAC	5 cut(s)	132, 261, 265, 1093, 1231
Mph1103I	ATGCAT	1 cut(s)	216
MroNI	GCCGGC	1 cut(s)	511
MseI	TTAA	2 cut(s)	534, 1716
MslI	CAYNNNNRTG	1 cut(s)	1028
MspA1I	CMGCKG	1 cut(s)	599
MspI	CCGG	5 cut(s)	31, 512, 538, 744, 1115
MspR9I	CCNGG	2 cut(s)	744, 1292
MunI	CAATTG	1 cut(s)	574
MvaI	CCWGG	1 cut(s)	1292
MvnI	CGCG	3 cut(s)	173, 1196, 1242
MwoI	GCNNNNNNNGC	4 cut(s)	512, 521, 808, 1237
NaeI	GCCGGC	1 cut(s)	513
NarI	GGCGCC	1 cut(s)	1118
NciI	CCSGG	1 cut(s)	744
NcoI	CCATGG	1 cut(s)	947
NdeII	GATC	7 cut(s)	220, 260, 461, 765, 1203, 1524, 1699
NgoMIV	GCCGGC	1 cut(s)	511
NlaIV	GGNNCC	5 cut(s)	463, 934, 1102, 1113, 1119
NmuCI	GTSAC	3 cut(s)	616, 746, 1534
NruI	TCGCGA	2 cut(s)	173, 1196
NsiI	ATGCAT	1 cut(s)	216
NspI	RCATGY	2 cut(s)	330, 1396
PaeR7I	CTCGAG	1 cut(s)	25
PciI	ACATGT	1 cut(s)	1392
PdiI	GCCGGC	1 cut(s)	513
PfeI	GAWTC	3 cut(s)	165, 318, 854
PluTI	GGCGCC	1 cut(s)	1121
Ppu21I	YACGTR	1 cut(s)	208
PpuMI	RGGWCCY	1 cut(s)	988
PscI	ACATGT	1 cut(s)	1392
Psp124BI	GAGCTC	2 cut(s)	824, 1508
Psp5II	RGGWCCY	1 cut(s)	988
Psp6I	CCWGG	1 cut(s)	1290
PspFI	CCCAGC	1 cut(s)	1216
PspGI	CCWGG	1 cut(s)	1290
PspN4I	GGNNCC	5 cut(s)	463, 934, 1102, 1113, 1119
PspPI	GGNCC	3 cut(s)	988, 1087, 1288
PspPPI	RGGWCCY	1 cut(s)	988
PspXI	VCTCGAGB	1 cut(s)	25
PstI	CTGCAG	1 cut(s)	1713
PsuI	RGATCY	2 cut(s)	461, 1699
PvuII	CAGCTG	1 cut(s)	599
RruI	TCGCGA	2 cut(s)	173, 1196
RsaI	GTAC	9 cut(s)	197, 206, 592, 656, 1172, 1178, 1496, 1676, 1682
RsaNI	GTAC	9 cut(s)	196, 205, 591, 655, 1171, 1177, 1495, 1675, 1681
RseI	CAYNNNNRTG	1 cut(s)	1028
SacI	GAGCTC	2 cut(s)	824, 1508
SaqAI	TTAA	2 cut(s)	534, 1716
Sau3AI	GATC	7 cut(s)	220, 260, 461, 765, 1203, 1524, 1699
Sau96I	GGNCC	3 cut(s)	988, 1087, 1288
ScaI	AGTACT	2 cut(s)	592, 1178
ScrFI	CCNGG	2 cut(s)	744, 1292
SduI	GDGCHC	4 cut(s)	56, 824, 1103, 1508
SfaNI	GCATC	3 cut(s)	201, 1089, 1647
SfcI	CTRYAG	3 cut(s)	15, 594, 1709
SfoI	GGCGCC	1 cut(s)	1119
Sfr274I	CTCGAG	1 cut(s)	25
SinI	GGWCC	2 cut(s)	988, 1087
SlaI	CTCGAG	1 cut(s)	25
SmiMI	CAYNNNNRTG	1 cut(s)	1028
SmlI	CTYRAG	3 cut(s)	25, 310, 1501
SmoI	CTYRAG	3 cut(s)	25, 310, 1501
SpeI	ACTAGT	1 cut(s)	1255
SsiI	CCGC	6 cut(s)	109, 447, 809, 1266, 1280, 1286
SspDI	GGCGCC	1 cut(s)	1117
SspMI	CTAG	2 cut(s)	1256, 1403
SstI	GAGCTC	2 cut(s)	824, 1508
StyD4I	CCNGG	2 cut(s)	742, 1290
StyI	CCWWGG	2 cut(s)	433, 947
TaaI	ACNGT	3 cut(s)	834, 964, 1170
TaiI	ACGT	2 cut(s)	210, 569
TaqI	TCGA	1 cut(s)	26
TatI	WGTACW	4 cut(s)	195, 590, 1176, 1680
TauI	GCSGC	1 cut(s)	449
TfiI	GAWTC	3 cut(s)	165, 318, 854
Tru1I	TTAA	2 cut(s)	534, 1716
Tru9I	TTAA	2 cut(s)	534, 1716
TscAI	CASTG	4 cut(s)	63, 486, 1173, 1810
TseFI	GTSAC	3 cut(s)	616, 746, 1534
Tsp45I	GTSAC	3 cut(s)	616, 746, 1534
TspGWI	ACGGA	1 cut(s)	881
TspRI	CASTG	4 cut(s)	63, 486, 1173, 1810
VpaK11BI	GGWCC	2 cut(s)	988, 1087
XagI	CCTNNNNNAGG	1 cut(s)	1295
XapI	RAATTY	2 cut(s)	129, 1418
XceI	RCATGY	2 cut(s)	330, 1396
XhoI	CTCGAG	1 cut(s)	25
XspI	CTAG	2 cut(s)	1256, 1403
ZrmI	AGTACT	2 cut(s)	592, 1178
Zsp2I	ATGCAT	1 cut(s)	216

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.