Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004855

Physical Location & Seq

Genomic Coordinates Forward (+)

10499065 .. 10501468

Gene Structure 2404 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1136700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1668 bp

ATGATGTTCCTGGATAACATAGGCTTTGTGGCTAACATGGCCAGCCTGGTTTTATACTTCATGTTCGTCATCCACTTCGACCTCTCTGGCTCTGCAACCACCACAACAAATTACTTGGGCACCACATTCTTGCTCACCATTGTTGGAGGATTCATCTCTGATGCCTACATGAACCGCCTCAACACGACTCTACTCTTTGGCGTCATCGAGTTGCTGGGATATATGTTGCTGATTATTCAAGCCCATGACAAAAACCTGCAACCTCTACCTTGTCTGGAATCAACCTGTGTGCATGGCACAAAAGCCCTACTGTTTTACGCTTCGATTTCCTTTGTGGCACTTGGAGGAGGCGGAATCAGAGGAGCAATCCCAGCTTTAGGAGCTGATCAATTCGACTCCAAAGATCCCAATGAGCACAAGCACATAGCCAGCTTCTTCAATTGGTTTTTGCTTAGCATAACAGTTGGAGCTGTGATTGGTGTCACATTTGTGGTGTATGTGAGCTCAAATGTTGGATGGGATATCGGATTTATCATCTCCTTGTCCTGCGCTGCTGTTGGTCTTCTCTTTGTTGCATTGGGAAAGCCATATTATCGCGTCCGAGTTCCAGGAGAGAGCCCTTTGATTAGGGTTTTAGAGGTACTGGTGGTGTCTGTAAAGAACTGGAAGGTGGAGGTACCCCAGAATTCCGATAAACTGTATGAAATACGTGCCCGCGATTCTGCTTCTAAAGCAGAGCTCATCCCTCACAGCAATCAATTCAGATTACTAGACAAGGCAGCCGTTCTCCCTAATGGCGCGAACGCAGCAAGAAAATGGAAAGTTTGCACAGTAACACAGGTGGAAGAAGTGAAGGTTTTGGCAAGGATGATGCCTATTCTTCTAAGCACAATCCTCATGAACACATGCTTAGCCCAATTGCAAACCCTTTCAATCCAACAAGGCACCCTCATGAACACACGCATTGGCGATTTTGATGTCCCATCCGCCACAATCCCAGTGATTCCTCTAGCGTTCATGACCCTCCTTATTCCAGTCTACGAATTTGCCTTCGTTCCCCTCTTGCGCAAAATCACGGGTCACCCCTATGGCATAACCCACCTCCAGAGAGTCGGGGTGGGGCTTGTCCTCTCAGCCATCTCAATGGCCATAGCAGGACTCGTAGAAGTTAAGCGAAAACACGAGATGGTTCACCACAACCACAAAATTAGCCTCTTCTGGCTCTCTTTCCATTACGCGATCTTTGGAATCGCGGACATGTTTACACTCGTAGGGCTGATGGAATTCTTCTACAAGGAGGCACCCGCCGGCATGCGCTCTCTTTCCACTTCCTTCTCTTGGCTCTCTCTGTCCATTGGCTACTACTTGAGTACGGTTTTCGTCGAGCTCATTAACAACATCACTAGCAAGTTCACCAAGAACAAAATGGGGTGGCTGGAAGGACGCGATATGAACAAAAGCCACGCGGACTATTTCTATTGGTTCCTCGCGATCCTCAGCGTGGTTAATTTCGGAATTTATGTATGGTGTGCCCAATGGTATAAGTATAAGAATGATGTTACAGTCGACGAGGAAATGTTGCTCAAAGCCAGCGGACCTCAGTCTTTCTCTGCAAGCAGTGTAAGCTTTGTGTCCAAGGTAGAAGAAAAACAAAACGAGGCCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PTR2

Protein Analysis

556

Amino Acids

61.73

Weight (kDa)

8.53

Isoelectric Point (pI)

31.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	3 - 454	8.4e-08	Major Facilitator Superfamily
PTR2	PF00854	61 - 478	8.9e-83	POT family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004855)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g21811 FvH4_4g21812 FvH4_6g08650 FvH4_6g08650
malus_domestica	MD04G1184500.v1.1 MD12G1197600.v1.1 MD12G1197700.v1.1 MD13G1136700.v1.1 MD16G1132000.v1.1
prunus_persica	Prupe.1G208700_v2.0.a1 Prupe.6G303000_v2.0.a1
pyrus_communis	pycom04g16360
rosa_chinensis	RchiOBHm_Chr3g0458781 RchiOBHm_Chr4g0428051
rosa_laevigata	RLG00000007186 RLG00000025135
rosa_multiflora	Rmu_co8474865.1_g000001 Rmu_sc0001634.1_g000029
rosa_roxburghii	Rroxscaffold_5G00369770 Rroxscaffold_6G00420790
rosa_rugosa	Rorug03G0031800
rosa_samantha	Rh3AG089500 Rh3BG092400 Rh3CG092700 Rh3DG093400 Rh4AG279100 Rh4BG284500 Rh4CG300200 Rh4DG281600
rosa_wichuraiana	Rw3G007660 Rw4G024250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1067
Acc36I	ACCTGC	1 cut(s)	264
Acc65I	GGTACC	1 cut(s)	676
AccB1I	GGYRCC	4 cut(s)	119, 676, 944, 1300
AccI	GTMKAC	2 cut(s)	1038, 1566
AccII	CGCG	8 cut(s)	597, 717, 800, 1238, 1253, 1446, 1466, 1490
AciI	CCGC	8 cut(s)	175, 351, 715, 987, 1253, 1305, 1466, 1593
AclWI	GGATC	2 cut(s)	398, 1486
AcoI	YGGCCR	2 cut(s)	39, 1146
AcsI	RAATTY	4 cut(s)	685, 1043, 1283, 1515
AcyI	GRCGYC	1 cut(s)	201
AfaI	GTAC	3 cut(s)	642, 678, 1372
AfiI	CCNNNNNNNGG	3 cut(s)	377, 1338, 1501
AflIII	ACRYGT	1 cut(s)	1257
AgsI	TTSAA	3 cut(s)	239, 439, 933
AjnI	CCWGG	3 cut(s)	9, 45, 607
AleI	CACNNNNGTG	1 cut(s)	488
AluBI	AGCT	8 cut(s)	374, 383, 432, 470, 504, 739, 1387, 1626
AluI	AGCT	8 cut(s)	374, 383, 432, 470, 504, 739, 1387, 1626
Alw21I	GWGCWC	4 cut(s)	417, 506, 741, 1389
AlwI	GGATC	2 cut(s)	398, 1486
AoxI	GGCC	3 cut(s)	39, 1146, 1659
ApeKI	GCWGC	3 cut(s)	551, 779, 806
ApoI	RAATTY	4 cut(s)	685, 1043, 1283, 1515
Asp718I	GGTACC	1 cut(s)	676
AspLEI	GCGC	4 cut(s)	551, 800, 1068, 1317
AspS9I	GGNCC	1 cut(s)	1595
AsuHPI	GGTGA	4 cut(s)	127, 1073, 1184, 1405
AvaII	GGWCC	1 cut(s)	1595
BaeGI	GKGCMC	3 cut(s)	122, 715, 1534
BalI	TGGCCA	2 cut(s)	41, 1148
BanI	GGYRCC	4 cut(s)	119, 676, 944, 1300
BanII	GRGCYC	4 cut(s)	506, 620, 741, 1389
BauI	CACGAG	1 cut(s)	1181
BbsI	GAAGAC	1 cut(s)	554
Bbv12I	GWGCWC	4 cut(s)	417, 506, 741, 1389
BbvCI	CCTCAGC	1 cut(s)	1496
BbvI	GCAGC	3 cut(s)	538, 791, 818
BccI	CCATC	5 cut(s)	510, 991, 1145, 1180, 1273
BceAI	ACGGC	1 cut(s)	767
BciT130I	CCWGG	3 cut(s)	11, 47, 609
BclI	TGATCA	1 cut(s)	385
BfaI	CTAG	3 cut(s)	770, 1010, 1404
BfuAI	ACCTGC	1 cut(s)	264
BisI	GCNGC	3 cut(s)	552, 780, 807
BlpI	GCTNAGC	2 cut(s)	452, 910
BlsI	GCNGC	3 cut(s)	553, 781, 808
Bme1390I	CCNGG	3 cut(s)	11, 47, 609
Bme18I	GGWCC	1 cut(s)	1595
BmgT120I	GGNCC	1 cut(s)	1595
BmiI	GGNNCC	5 cut(s)	121, 678, 946, 1302, 1484
BmrFI	CCNGG	3 cut(s)	11, 47, 609
BmrI	ACTGGG	1 cut(s)	992
BmsI	GCATC	2 cut(s)	151, 861
BmuI	ACTGGG	1 cut(s)	992
BoxI	GACNNNNGTC	1 cut(s)	1600
BpiI	GAAGAC	1 cut(s)	554
BpmI	CTGGAG	1 cut(s)	1088
Bpu10I	CCTNAGC	1 cut(s)	1496
Bpu1102I	GCTNAGC	2 cut(s)	452, 910
BpuEI	CTTGAG	1 cut(s)	1387
BsaAI	YACGTR	1 cut(s)	710
BsaHI	GRCGYC	1 cut(s)	201
BsaJI	CCNNGG	1 cut(s)	1635
Bsc4I	CCNNNNNNNGG	3 cut(s)	377, 1338, 1501
Bse118I	RCCGGY	1 cut(s)	1307
Bse1I	ACTGG	4 cut(s)	648, 668, 998, 1034
BseBI	CCWGG	3 cut(s)	11, 47, 609
BseDI	CCNNGG	1 cut(s)	1635
BseGI	GGATG	5 cut(s)	69, 521, 741, 873, 983
BseLI	CCNNNNNNNGG	3 cut(s)	377, 1338, 1501
BseMII	CTCAG	3 cut(s)	1146, 1510, 1613
BseNI	ACTGG	4 cut(s)	648, 668, 998, 1034
BseRI	GAGGAG	2 cut(s)	360, 375
BseSI	GKGCMC	3 cut(s)	122, 715, 1534
BseXI	GCAGC	3 cut(s)	538, 791, 818
BseYI	CCCAGC	2 cut(s)	214, 370
Bsh1236I	CGCG	8 cut(s)	597, 717, 800, 1238, 1253, 1446, 1466, 1490
BshFI	GGCC	3 cut(s)	41, 1148, 1661
BshNI	GGYRCC	4 cut(s)	119, 676, 944, 1300
BsiHKAI	GWGCWC	4 cut(s)	417, 506, 741, 1389
BsiSI	CCGG	1 cut(s)	1308
BslFI	GGGAC	1 cut(s)	965
BslI	CCNNNNNNNGG	3 cut(s)	377, 1338, 1501
BsmFI	GGGAC	1 cut(s)	965
BsnI	GGCC	3 cut(s)	41, 1148, 1661
Bsp1286I	GDGCHC	8 cut(s)	122, 417, 506, 620, 715, 741, 1389, 1534
Bsp143I	GATC	4 cut(s)	385, 403, 1239, 1491
Bsp1720I	GCTNAGC	2 cut(s)	452, 910
Bsp68I	TCGCGA	1 cut(s)	1490
BspACI	CCGC	8 cut(s)	175, 351, 715, 987, 1253, 1305, 1466, 1593
BspANI	GGCC	3 cut(s)	41, 1148, 1661
BspCNI	CTCAG	3 cut(s)	1145, 1509, 1612
BspFNI	CGCG	8 cut(s)	597, 717, 800, 1238, 1253, 1446, 1466, 1490
BspHI	TCATGA	3 cut(s)	897, 951, 1017
BspLI	GGNNCC	5 cut(s)	121, 678, 946, 1302, 1484
BspMI	ACCTGC	1 cut(s)	264
BspPI	GGATC	2 cut(s)	398, 1486
BspT107I	GGYRCC	4 cut(s)	119, 676, 944, 1300
BsrFI	RCCGGY	1 cut(s)	1307
BsrI	ACTGG	4 cut(s)	648, 668, 998, 1034
BssAI	RCCGGY	1 cut(s)	1307
BssECI	CCNNGG	1 cut(s)	1635
BssMI	GATC	4 cut(s)	385, 403, 1239, 1491
BssNI	GRCGYC	1 cut(s)	201
BssSI	CACGAG	1 cut(s)	1181
BssT1I	CCWWGG	1 cut(s)	1635
Bst2BI	CACGAG	1 cut(s)	1181
Bst2UI	CCWGG	3 cut(s)	11, 47, 609
Bst4CI	ACNGT	6 cut(s)	312, 463, 699, 832, 1375, 1564
Bst6I	CTCTTC	1 cut(s)	1220
BstACI	GRCGYC	1 cut(s)	201
BstBAI	YACGTR	1 cut(s)	710
BstC8I	GCNNGC	7 cut(s)	43, 430, 715, 1309, 1313, 1591, 1615
BstDEI	CTNAG	6 cut(s)	452, 884, 910, 1132, 1496, 1599
BstEII	GGTNACC	1 cut(s)	1079
BstF5I	GGATG	5 cut(s)	69, 521, 741, 873, 983
BstFNI	CGCG	8 cut(s)	597, 717, 800, 1238, 1253, 1446, 1466, 1490
BstHHI	GCGC	4 cut(s)	551, 800, 1068, 1317
BstKTI	GATC	4 cut(s)	388, 406, 1242, 1494
BstMBI	GATC	4 cut(s)	385, 403, 1239, 1491
BstMWI	GCNNNNNNNGC	6 cut(s)	38, 371, 380, 731, 806, 1623
BstNI	CCWGG	3 cut(s)	11, 47, 609
BstNSI	RCATGY	3 cut(s)	909, 1261, 1315
BstPAI	GACNNNNGTC	1 cut(s)	1600
BstPI	GGTNACC	1 cut(s)	1079
BstSCI	CCNGG	3 cut(s)	9, 45, 607
BstSLI	GKGCMC	3 cut(s)	122, 715, 1534
BstUI	CGCG	8 cut(s)	597, 717, 800, 1238, 1253, 1446, 1466, 1490
BstV1I	GCAGC	3 cut(s)	538, 791, 818
BstV2I	GAAGAC	1 cut(s)	554
BstX2I	RGATCY	1 cut(s)	403
BstXI	CCANNNNNNTGG	1 cut(s)	1144
BstYI	RGATCY	1 cut(s)	403
BsuRI	GGCC	3 cut(s)	41, 1148, 1661
BtsCI	GGATG	5 cut(s)	69, 521, 741, 873, 983
BtsI	GCAGTG	1 cut(s)	1624
BtsIMutI	CAGTG	2 cut(s)	1005, 1624
BtuMI	TCGCGA	1 cut(s)	1490
BveI	ACCTGC	1 cut(s)	264
Cac8I	GCNNGC	7 cut(s)	43, 430, 715, 1309, 1313, 1591, 1615
CciI	TCATGA	3 cut(s)	897, 951, 1017
CfoI	GCGC	4 cut(s)	551, 800, 1068, 1317
Cfr10I	RCCGGY	1 cut(s)	1307
Cfr13I	GGNCC	1 cut(s)	1595
CseI	GACGC	3 cut(s)	190, 586, 1452
Csp6I	GTAC	3 cut(s)	641, 677, 1371
CviQI	GTAC	3 cut(s)	641, 677, 1371
DdeI	CTNAG	6 cut(s)	452, 884, 910, 1132, 1496, 1599
DpnI	GATC	4 cut(s)	387, 405, 1241, 1493
DpnII	GATC	4 cut(s)	385, 403, 1239, 1491
EaeI	YGGCCR	2 cut(s)	39, 1146
Eam1104I	CTCTTC	1 cut(s)	1220
EarI	CTCTTC	1 cut(s)	1220
EciI	GGCGGA	2 cut(s)	366, 976
Ecl136II	GAGCTC	3 cut(s)	504, 739, 1387
Eco130I	CCWWGG	1 cut(s)	1635
Eco24I	GRGCYC	4 cut(s)	506, 620, 741, 1389
Eco32I	GATATC	1 cut(s)	523
Eco47I	GGWCC	1 cut(s)	1595
Eco53kI	GAGCTC	3 cut(s)	504, 739, 1387
Eco91I	GGTNACC	1 cut(s)	1079
EcoICRI	GAGCTC	3 cut(s)	504, 739, 1387
EcoO65I	GGTNACC	1 cut(s)	1079
EcoRI	GAATTC	2 cut(s)	685, 1283
EcoRII	CCWGG	3 cut(s)	9, 45, 607
EcoRV	GATATC	1 cut(s)	523
EcoT14I	CCWWGG	1 cut(s)	1635
EcoT38I	GRGCYC	4 cut(s)	506, 620, 741, 1389
ErhI	CCWWGG	1 cut(s)	1635
FaqI	GGGAC	1 cut(s)	965
FauI	CCCGC	2 cut(s)	722, 1312
FbaI	TGATCA	1 cut(s)	385
FblI	GTMKAC	2 cut(s)	1038, 1566
Fnu4HI	GCNGC	3 cut(s)	552, 780, 807
FokI	GGATG	5 cut(s)	56, 528, 728, 880, 970
FriOI	GRGCYC	4 cut(s)	506, 620, 741, 1389
Fsp4HI	GCNGC	3 cut(s)	552, 780, 807
FspBI	CTAG	3 cut(s)	770, 1010, 1404
FspI	TGCGCA	1 cut(s)	1067
GlaI	GCGC	4 cut(s)	550, 799, 1067, 1316
GluI	GCNGC	3 cut(s)	552, 780, 807
GsaI	CCCAGC	2 cut(s)	218, 374
GsuI	CTGGAG	1 cut(s)	1088
HaeIII	GGCC	3 cut(s)	41, 1148, 1661
HapII	CCGG	1 cut(s)	1308
HgaI	GACGC	3 cut(s)	190, 586, 1452
HhaI	GCGC	4 cut(s)	551, 800, 1068, 1317
Hin1I	GRCGYC	1 cut(s)	201
Hin6I	GCGC	4 cut(s)	549, 798, 1066, 1315
HinP1I	GCGC	4 cut(s)	549, 798, 1066, 1315
HincII	GTYRAC	1 cut(s)	1567
HindII	GTYRAC	1 cut(s)	1567
HindIII	AAGCTT	1 cut(s)	1624
HpaII	CCGG	1 cut(s)	1308
HphI	GGTGA	4 cut(s)	127, 1073, 1184, 1405
Hpy166II	GTNNAC	5 cut(s)	1039, 1192, 1263, 1413, 1567
Hpy188I	TCNGA	7 cut(s)	160, 359, 527, 602, 691, 764, 1514
Hpy188III	TCNNGA	6 cut(s)	275, 898, 952, 1018, 1105, 1489
Hpy8I	GTNNAC	5 cut(s)	1039, 1192, 1263, 1413, 1567
Hpy99I	CGWCG	2 cut(s)	1385, 1571
HpyAV	CCTTC	5 cut(s)	661, 847, 1060, 1342, 1433
HpyCH4III	ACNGT	6 cut(s)	312, 463, 699, 832, 1375, 1564
HpyCH4IV	ACGT	1 cut(s)	709
HpyCH4V	TGCA	7 cut(s)	95, 259, 292, 575, 828, 922, 1613
HpyF10VI	GCNNNNNNNGC	6 cut(s)	38, 371, 380, 731, 806, 1623
HpyF3I	CTNAG	6 cut(s)	452, 884, 910, 1132, 1496, 1599
HpySE526I	ACGT	1 cut(s)	709
Hsp92I	GRCGYC	1 cut(s)	201
HspAI	GCGC	4 cut(s)	549, 798, 1066, 1315
KpnI	GGTACC	1 cut(s)	680
KroI	GCCGGC	1 cut(s)	1307
KroNI	GCCGGC	1 cut(s)	1309
Ksp22I	TGATCA	1 cut(s)	385
Kzo9I	GATC	4 cut(s)	385, 403, 1239, 1491
LmnI	GCTCC	3 cut(s)	362, 380, 467
Lsp1109I	GCAGC	3 cut(s)	538, 791, 818
LweI	GCATC	2 cut(s)	151, 861
MaeI	CTAG	3 cut(s)	770, 1010, 1404
MaeII	ACGT	1 cut(s)	709
MaeIII	GTNAC	4 cut(s)	481, 832, 1079, 1558
MalI	GATC	4 cut(s)	387, 405, 1241, 1493
MboI	GATC	4 cut(s)	385, 403, 1239, 1491
MboII	GAAGA	7 cut(s)	427, 554, 857, 872, 1207, 1279, 1655
MfeI	CAATTG	2 cut(s)	439, 917
MflI	RGATCY	1 cut(s)	403
MhlI	GDGCHC	8 cut(s)	122, 417, 506, 620, 715, 741, 1389, 1534
MlsI	TGGCCA	2 cut(s)	41, 1148
MluNI	TGGCCA	2 cut(s)	41, 1148
MlyI	GAGTC	4 cut(s)	181, 389, 1119, 1152
MmeI	TCCRAC	4 cut(s)	124, 445, 493, 961
Mox20I	TGGCCA	2 cut(s)	41, 1148
MroNI	GCCGGC	1 cut(s)	1307
MscI	TGGCCA	2 cut(s)	41, 1148
MseI	TTAA	3 cut(s)	1170, 1392, 1506
MslI	CAYNNNNRTG	4 cut(s)	488, 950, 1086, 1142
Msp20I	TGGCCA	2 cut(s)	41, 1148
MspA1I	CMGCKG	1 cut(s)	1593
MspI	CCGG	1 cut(s)	1308
MspR9I	CCNGG	3 cut(s)	11, 47, 609
MunI	CAATTG	2 cut(s)	439, 917
MvaI	CCWGG	3 cut(s)	11, 47, 609
MvnI	CGCG	8 cut(s)	597, 717, 800, 1238, 1253, 1446, 1466, 1490
MwoI	GCNNNNNNNGC	6 cut(s)	38, 371, 380, 731, 806, 1623
NaeI	GCCGGC	1 cut(s)	1309
NdeII	GATC	4 cut(s)	385, 403, 1239, 1491
NgoMIV	GCCGGC	1 cut(s)	1307
NlaIV	GGNNCC	5 cut(s)	121, 678, 946, 1302, 1484
NmuCI	GTSAC	2 cut(s)	481, 1079
NruI	TCGCGA	1 cut(s)	1490
NsbI	TGCGCA	1 cut(s)	1067
NspI	RCATGY	3 cut(s)	909, 1261, 1315
OliI	CACNNNNGTG	1 cut(s)	488
PaeI	GCATGC	1 cut(s)	1315
PagI	TCATGA	3 cut(s)	897, 951, 1017
PciI	ACATGT	1 cut(s)	1257
PdiI	GCCGGC	1 cut(s)	1309
PfeI	GAWTC	6 cut(s)	150, 278, 354, 719, 1003, 1248
PfoI	TCCNGGA	2 cut(s)	9, 607
PkrI	GCNGC	3 cut(s)	553, 781, 808
PleI	GAGTC	4 cut(s)	181, 389, 1118, 1152
PpsI	GAGTC	4 cut(s)	181, 389, 1118, 1152
Ppu21I	YACGTR	1 cut(s)	710
PscI	ACATGT	1 cut(s)	1257
PshAI	GACNNNNGTC	1 cut(s)	1600
Psp124BI	GAGCTC	3 cut(s)	506, 741, 1389
Psp6I	CCWGG	3 cut(s)	9, 45, 607
PspEI	GGTNACC	1 cut(s)	1079
PspFI	CCCAGC	2 cut(s)	214, 370
PspGI	CCWGG	3 cut(s)	9, 45, 607
PspN4I	GGNNCC	5 cut(s)	121, 678, 946, 1302, 1484
PspPI	GGNCC	1 cut(s)	1595
PsuI	RGATCY	1 cut(s)	403
RruI	TCGCGA	1 cut(s)	1490
RsaI	GTAC	3 cut(s)	642, 678, 1372
RsaNI	GTAC	3 cut(s)	641, 677, 1371
RseI	CAYNNNNRTG	4 cut(s)	488, 950, 1086, 1142
SacI	GAGCTC	3 cut(s)	506, 741, 1389
SalI	GTCGAC	1 cut(s)	1565
SaqAI	TTAA	3 cut(s)	1170, 1392, 1506
SatI	GCNGC	3 cut(s)	552, 780, 807
Sau3AI	GATC	4 cut(s)	385, 403, 1239, 1491
Sau96I	GGNCC	1 cut(s)	1595
SchI	GAGTC	4 cut(s)	181, 389, 1119, 1152
ScrFI	CCNGG	3 cut(s)	11, 47, 609
SduI	GDGCHC	8 cut(s)	122, 417, 506, 620, 715, 741, 1389, 1534
SfaNI	GCATC	2 cut(s)	151, 861
SinI	GGWCC	1 cut(s)	1595
SmiMI	CAYNNNNRTG	4 cut(s)	488, 950, 1086, 1142
SmlI	CTYRAG	1 cut(s)	1366
SmoI	CTYRAG	1 cut(s)	1366
SphI	GCATGC	1 cut(s)	1315
SsiI	CCGC	8 cut(s)	175, 351, 715, 987, 1253, 1305, 1466, 1593
SspMI	CTAG	3 cut(s)	770, 1010, 1404
SstI	GAGCTC	3 cut(s)	506, 741, 1389
StyD4I	CCNGG	3 cut(s)	9, 45, 607
StyI	CCWWGG	1 cut(s)	1635
TaaI	ACNGT	6 cut(s)	312, 463, 699, 832, 1375, 1564
TaiI	ACGT	1 cut(s)	712
TaqI	TCGA	6 cut(s)	78, 207, 323, 393, 1383, 1566
TfiI	GAWTC	6 cut(s)	150, 278, 354, 719, 1003, 1248
Tru1I	TTAA	3 cut(s)	1170, 1392, 1506
Tru9I	TTAA	3 cut(s)	1170, 1392, 1506
TscAI	CASTG	2 cut(s)	1005, 1624
TseFI	GTSAC	2 cut(s)	481, 1079
TseI	GCWGC	3 cut(s)	551, 779, 806
Tsp45I	GTSAC	2 cut(s)	481, 1079
TspDTI	ATGAA	8 cut(s)	49, 142, 185, 717, 914, 968, 1006, 1466
TspRI	CASTG	2 cut(s)	1005, 1624
VpaK11BI	GGWCC	1 cut(s)	1595
XapI	RAATTY	4 cut(s)	685, 1043, 1283, 1515
XceI	RCATGY	3 cut(s)	909, 1261, 1315
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1423
XmiI	GTMKAC	2 cut(s)	1038, 1566
XspI	CTAG	3 cut(s)	770, 1010, 1404

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.