Rw4G024250

Protein NRT1 PTR FAMILY 4.5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004855
Physical Location & Seq
Reverse (-)
50118953 .. 50121754
2802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024250.1

Sequence Viewer

Length: 1821 bp
ATGATTGGGAGTATATTAGAGCTGAAGTTGCTGTCAAAAGCTACGAGAATGGATATTAGTGTTATTGGATTGGGGTTGGACAGTGAAATTGGAAATAGATCGAACAAAGAGCTAGCTTATGAATCTATGAAGAAACAAGAAAGAGAAGGCGGATTTAAGGCCAACTATTTCATCTTTGCAATGATGTTTCTGGATAACATAGGGTTTGTGGCTAACATGGCAAGCCTGGTTCTATACTTTATGCTTGTGATACACTTTGACATCTCTGGCTCTGCTACCACCACAACAAACTACTTGGGTTCTGCATTCTTGCTTACATTAGTTGGAGGATTCATCTCTGATGCCTACATGAATCGCCTCAACACCACTCTACTCTTCGGTGTGGCAGAACTGTTGGGATATTCATTGTTGATTATTCAATCCCATTATACAAAACTGCAACCTTTACCATGTGGAGAATCAACCTGCGTTCATGGCACAAAAGCCTTGCTGTTTTATGCTTCAATATCCTTAGTTGCACTGGGAGGAGGTGGAATCAGAGGAGCGATTCCTGCTTTAGGTGCTGACCAGTTCAACTACAAGAAACCCGAAGAGCGCAAGCATATAGCTAGCTTTTTCAACTGGCTTTTGCTTAGTATAACCGTTGGGGCTGTGGTTGGAGTCACATTTGTGGTATATGTCAGCACAACGGTTGGATGGGACATAGCATTTATCATCTCCTTGTCTTGTGCTGGTGCTGGTCTTGTCATTGTTGCTTTGGGAAAGCCATATTATCGTGTTCGAGTTGCAGGAGAAAGTCCTTTACTAAGGGTTTTACAGGTTTTGGTGGCAAGTGTAAAGAACTGGAGGGTTCAGGTGCCTCAGAGTCCTGATGAACTACATGAATCCACTAAAAAGAGAGAGAGAATCACCCACACTGACCAATTCAGATTACTAGACAAGGCTGCCATTCTCCCTAACGGTGTCGATGCTGCAATTGCATCAAAATGGAAAGTTTGTACAGTAACACAGGTGGAGGAAGTGAAGATCCTCACAAGGATGATGCCTATTCTTCTAAGCACAACCCTCATGAACACATGCTTGGCACAACTGCAAACTCTTTCCGTCCAACAAGGAACCCTCATGGACACACGCATTGGTAGTTTTAATGTTCCAGCAGCCACAATTCCAGTCATTCCTCTACTATTCATGTCTGTTCTTATCCCCACCTACGAGTTCACTTTCGTTCCCCTTTTGCGCAAGTTCACTGGTCACCCCAATGGCATAACCCATCTCCAGAGAGTTGGTGTTGGGCTTGTTCTGTCAGCTATCTCCATGACTATAGCGGGGTTCGTGGAAGTGAAGAGAAAGCATGAGTTCATTCACCACAATCACAAGATCAGCCTCTTTTGGCTCTCATTTCACTATGCAATTTTCGGCATTGCAGACATGTTCACACTTGTGGGGTTGATGGAGTTCTTCTATAGAGAAGCTCCTGCTGGCATGAGGTCTCTGTCTACTTCATTTTCTTGGCTTTCTCTCTCAATTGGGTACTACCTGAGCTCAGCTTTTGTAGAGCTCATTAACTCTATCACTGGCAAGTATACAGACAGCAAGTTAGGGTGGCTAGAGGGGCTTGACATGAACAAGAACCACATAGAACTTTTCTACTGGTTTTTGGCGATTCTGAGCGTGCTTAACTTTGCAAATTATCTTTTCTGGGCCAACTGGTACACGTATAAGAACGATGTTACAGTCGATGAGGAAATGCTAATCAAAGCAGGCCAAGTTGGAGCTGATGAATCTAACTCACTAAGTGTAAGCACTGTGTCCAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

606

Amino Acids

67.19

Weight (kDa)

8.56

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 121 - 527 1.9e-79 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1238
Acc36I ACCTGC 1 cut(s) 473
AccB1I GGYRCC 1 cut(s) 856
AccI GTMKAC 2 cut(s) 1496, 1583
AciI CCGC 2 cut(s) 150, 1325
AclWI GGATC 1 cut(s) 1021
AcuI CTGAAG 1 cut(s) 44
AdeI CACNNNGTG 1 cut(s) 1796
AfaI GTAC 3 cut(s) 1000, 1532, 1712
AfiI CCNNNNNNNGG 1 cut(s) 557
AflIII ACRYGT 2 cut(s) 1428, 1713
AgsI TTSAA 4 cut(s) 419, 504, 574, 619
AjnI CCWGG 1 cut(s) 225
AjuI GAANNNNNNNTTGG 2 cut(s) 1064, 1096
AleI CACNNNNGTG 2 cut(s) 668, 1439
Alw21I GWGCWC 2 cut(s) 1544, 1560
Alw26I GTCTC 1 cut(s) 1494
AlwI GGATC 1 cut(s) 1021
AoxI GGCC 3 cut(s) 159, 1701, 1762
ApeKI GCWGC 3 cut(s) 944, 971, 1157
AspLEI GCGC 2 cut(s) 597, 1239
AspS9I GGNCC 1 cut(s) 1701
AsuHPI GGTGA 3 cut(s) 901, 1244, 1355
AsuNHI GCTAGC 2 cut(s) 112, 608
BanI GGYRCC 1 cut(s) 856
BanII GRGCYC 2 cut(s) 1544, 1560
Bbv12I GWGCWC 2 cut(s) 1544, 1560
BbvI GCAGC 3 cut(s) 931, 958, 1169
BccI CCATC 3 cut(s) 690, 1278, 1444
BciT130I CCWGG 1 cut(s) 227
BcoDI GTCTC 1 cut(s) 1494
BfaI CTAG 4 cut(s) 113, 609, 935, 1607
BfmI CTRYAG 2 cut(s) 1320, 1462
BfuAI ACCTGC 1 cut(s) 473
BisI GCNGC 3 cut(s) 945, 972, 1158
BlpI GCTNAGC 1 cut(s) 1543
BlsI GCNGC 3 cut(s) 946, 973, 1159
Bme1390I CCNGG 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 1701
BmiI GGNNCC 2 cut(s) 858, 1117
BmrFI CCNGG 1 cut(s) 227
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 4 cut(s) 331, 958, 989, 1032
BmtI GCTAGC 2 cut(s) 116, 612
BmuI ACTGGG 1 cut(s) 530
BpmI CTGGAG 2 cut(s) 865, 1259
Bpu10I CCTNAGC 1 cut(s) 1538
Bpu1102I GCTNAGC 1 cut(s) 1543
BsaAI YACGTR 1 cut(s) 1716
BsaI GGTCTC 1 cut(s) 1494
Bsc4I CCNNNNNNNGG 1 cut(s) 557
Bse1I ACTGG 9 cut(s) 525, 568, 626, 848, 1169, 1252, 1579, 1655, 1712
Bse3DI GCAATG 2 cut(s) 186, 1419
BseBI CCWGG 1 cut(s) 227
BseGI GGATG 2 cut(s) 701, 1044
BseLI CCNNNNNNNGG 1 cut(s) 557
BseMI GCAATG 2 cut(s) 186, 1419
BseMII CTCAG 4 cut(s) 875, 1529, 1557, 1658
BseNI ACTGG 9 cut(s) 525, 568, 626, 848, 1169, 1252, 1579, 1655, 1712
BseRI GAGGAG 2 cut(s) 540, 555
BseXI GCAGC 3 cut(s) 931, 958, 1169
BshFI GGCC 3 cut(s) 161, 1703, 1764
BshNI GGYRCC 1 cut(s) 856
BsiHKAI GWGCWC 2 cut(s) 1544, 1560
BslFI GGGAC 1 cut(s) 713
BslI CCNNNNNNNGG 1 cut(s) 557
BsmAI GTCTC 1 cut(s) 1494
BsmFI GGGAC 1 cut(s) 713
BsmI GAATGC 1 cut(s) 305
BsnI GGCC 3 cut(s) 161, 1703, 1764
Bso31I GGTCTC 1 cut(s) 1494
Bsp1286I GDGCHC 2 cut(s) 1544, 1560
Bsp1407I TGTACA 1 cut(s) 998
Bsp143I GATC 3 cut(s) 98, 1026, 1377
Bsp1720I GCTNAGC 1 cut(s) 1543
BspACI CCGC 2 cut(s) 150, 1325
BspANI GGCC 3 cut(s) 161, 1703, 1764
BspCNI CTCAG 4 cut(s) 874, 1530, 1556, 1659
BspHI TCATGA 1 cut(s) 1068
BspLI GGNNCC 2 cut(s) 858, 1117
BspMI ACCTGC 1 cut(s) 473
BspOI GCTAGC 2 cut(s) 116, 612
BspPI GGATC 1 cut(s) 1021
BspQI GCTCTTC 1 cut(s) 585
BspT107I GGYRCC 1 cut(s) 856
BspTNI GGTCTC 1 cut(s) 1494
BsrDI GCAATG 2 cut(s) 186, 1419
BsrGI TGTACA 1 cut(s) 998
BsrI ACTGG 9 cut(s) 525, 568, 626, 848, 1169, 1252, 1579, 1655, 1712
BssMI GATC 3 cut(s) 98, 1026, 1377
BssNAI GTATAC 1 cut(s) 1584
Bst1107I GTATAC 1 cut(s) 1584
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 8 cut(s) 83, 393, 643, 691, 962, 1003, 1735, 1807
Bst6I CTCTTC 3 cut(s) 380, 585, 1337
BstAUI TGTACA 1 cut(s) 998
BstBAI YACGTR 1 cut(s) 1716
BstC8I GCNNGC 7 cut(s) 114, 223, 599, 610, 1480, 1673, 1762
BstDEI CTNAG 9 cut(s) 511, 632, 806, 861, 1055, 1538, 1543, 1667, 1793
BstEII GGTNACC 1 cut(s) 1250
BstENI CCTNNNNNAGG 1 cut(s) 555
BstF5I GGATG 2 cut(s) 701, 1044
BstHHI GCGC 2 cut(s) 597, 1239
BstKTI GATC 3 cut(s) 101, 1029, 1380
BstMAI GTCTC 1 cut(s) 1494
BstMBI GATC 3 cut(s) 98, 1026, 1377
BstMWI GCNNNNNNNGC 7 cut(s) 28, 218, 474, 551, 560, 977, 1612
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 2 cut(s) 1080, 1432
BstPI GGTNACC 1 cut(s) 1250
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 2 cut(s) 1320, 1462
BstV1I GCAGC 3 cut(s) 931, 958, 1169
BstX2I RGATCY 1 cut(s) 1026
BstXI CCANNNNNNTGG 1 cut(s) 1283
BstYI RGATCY 1 cut(s) 1026
BstZ17I GTATAC 1 cut(s) 1584
BsuRI GGCC 3 cut(s) 161, 1703, 1764
BtsCI GGATG 2 cut(s) 701, 1044
BtsIMutI CAGTG 6 cut(s) 88, 518, 915, 1245, 1572, 1803
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 7 cut(s) 114, 223, 599, 610, 1480, 1673, 1762
CciI TCATGA 1 cut(s) 1068
CfoI GCGC 2 cut(s) 597, 1239
Cfr13I GGNCC 1 cut(s) 1701
Csp6I GTAC 3 cut(s) 999, 1531, 1711
CviQI GTAC 3 cut(s) 999, 1531, 1711
DdeI CTNAG 9 cut(s) 511, 632, 806, 861, 1055, 1538, 1543, 1667, 1793
DpnI GATC 3 cut(s) 100, 1028, 1379
DpnII GATC 3 cut(s) 98, 1026, 1377
DraIII CACNNNGTG 1 cut(s) 1796
Eam1104I CTCTTC 3 cut(s) 380, 585, 1337
EarI CTCTTC 3 cut(s) 380, 585, 1337
EciI GGCGGA 1 cut(s) 165
Ecl136II GAGCTC 2 cut(s) 1542, 1558
Eco24I GRGCYC 2 cut(s) 1544, 1560
Eco31I GGTCTC 1 cut(s) 1494
Eco53kI GAGCTC 2 cut(s) 1542, 1558
Eco57I CTGAAG 1 cut(s) 44
Eco91I GGTNACC 1 cut(s) 1250
EcoICRI GAGCTC 2 cut(s) 1542, 1558
EcoNI CCTNNNNNAGG 1 cut(s) 555
EcoO65I GGTNACC 1 cut(s) 1250
EcoRII CCWGG 1 cut(s) 225
EcoT38I GRGCYC 2 cut(s) 1544, 1560
FaqI GGGAC 1 cut(s) 713
FauI CCCGC 1 cut(s) 1318
FblI GTMKAC 2 cut(s) 1496, 1583
Fnu4HI GCNGC 3 cut(s) 945, 972, 1158
FokI GGATG 2 cut(s) 708, 1051
FriOI GRGCYC 2 cut(s) 1544, 1560
Fsp4HI GCNGC 3 cut(s) 945, 972, 1158
FspBI CTAG 4 cut(s) 113, 609, 935, 1607
FspI TGCGCA 1 cut(s) 1238
GlaI GCGC 2 cut(s) 596, 1238
GluI GCNGC 3 cut(s) 945, 972, 1158
GsuI CTGGAG 2 cut(s) 865, 1259
HaeIII GGCC 3 cut(s) 161, 1703, 1764
HhaI GCGC 2 cut(s) 597, 1239
Hin6I GCGC 2 cut(s) 595, 1237
HinP1I GCGC 2 cut(s) 595, 1237
HphI GGTGA 3 cut(s) 901, 1244, 1355
Hpy166II GTNNAC 6 cut(s) 1218, 1245, 1434, 1497, 1584, 1713
Hpy188I TCNGA 5 cut(s) 340, 539, 864, 929, 1668
Hpy188III TCNNGA 5 cut(s) 191, 869, 1069, 1276, 1812
Hpy8I GTNNAC 6 cut(s) 1218, 1245, 1434, 1497, 1584, 1713
HpyAV CCTTC 1 cut(s) 140
HpyCH4III ACNGT 8 cut(s) 83, 393, 643, 691, 962, 1003, 1735, 1807
HpyCH4IV ACGT 1 cut(s) 1715
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 218, 474, 551, 560, 977, 1612
HpyF3I CTNAG 9 cut(s) 511, 632, 806, 861, 1055, 1538, 1543, 1667, 1793
HpySE526I ACGT 1 cut(s) 1715
HspAI GCGC 2 cut(s) 595, 1237
Kzo9I GATC 3 cut(s) 98, 1026, 1377
LguI GCTCTTC 1 cut(s) 585
LmnI GCTCC 3 cut(s) 542, 1477, 1772
Lsp1109I GCAGC 3 cut(s) 931, 958, 1169
LweI GCATC 4 cut(s) 331, 958, 989, 1032
MaeI CTAG 4 cut(s) 113, 609, 935, 1607
MaeII ACGT 1 cut(s) 1715
MaeIII GTNAC 4 cut(s) 661, 1003, 1250, 1729
MalI GATC 3 cut(s) 100, 1028, 1379
MboI GATC 3 cut(s) 98, 1026, 1377
MboII GAAGA 7 cut(s) 142, 367, 602, 1036, 1043, 1354, 1450
MfeI CAATTG 2 cut(s) 975, 1524
MflI RGATCY 1 cut(s) 1026
MhlI GDGCHC 2 cut(s) 1544, 1560
MluCI AATT 7 cut(s) 87, 923, 975, 1164, 1410, 1524, 1687
MlyI GAGTC 2 cut(s) 669, 874
MmeI TCCRAC 6 cut(s) 57, 304, 637, 673, 1132, 1750
MseI TTAA 4 cut(s) 156, 1146, 1563, 1677
MslI CAYNNNNRTG 5 cut(s) 668, 985, 1037, 1257, 1439
MspR9I CCNGG 1 cut(s) 227
MunI CAATTG 2 cut(s) 975, 1524
Mva1269I GAATGC 1 cut(s) 305
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 7 cut(s) 28, 218, 474, 551, 560, 977, 1612
NdeII GATC 3 cut(s) 98, 1026, 1377
NheI GCTAGC 2 cut(s) 112, 608
NlaIV GGNNCC 2 cut(s) 858, 1117
NmuCI GTSAC 2 cut(s) 661, 1250
NsbI TGCGCA 1 cut(s) 1238
NspI RCATGY 2 cut(s) 1080, 1432
OliI CACNNNNGTG 2 cut(s) 668, 1439
PagI TCATGA 1 cut(s) 1068
PciI ACATGT 1 cut(s) 1428
PciSI GCTCTTC 1 cut(s) 585
PctI GAATGC 1 cut(s) 305
PkrI GCNGC 3 cut(s) 946, 973, 1159
PleI GAGTC 2 cut(s) 668, 873
PpsI GAGTC 2 cut(s) 668, 873
Ppu21I YACGTR 1 cut(s) 1716
PscI ACATGT 1 cut(s) 1428
Psp124BI GAGCTC 2 cut(s) 1544, 1560
Psp6I CCWGG 1 cut(s) 225
PspEI GGTNACC 1 cut(s) 1250
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 2 cut(s) 858, 1117
PspPI GGNCC 1 cut(s) 1701
PsuI RGATCY 1 cut(s) 1026
RsaI GTAC 3 cut(s) 1000, 1532, 1712
RsaNI GTAC 3 cut(s) 999, 1531, 1711
RseI CAYNNNNRTG 5 cut(s) 668, 985, 1037, 1257, 1439
SacI GAGCTC 2 cut(s) 1544, 1560
SapI GCTCTTC 1 cut(s) 585
SaqAI TTAA 4 cut(s) 156, 1146, 1563, 1677
SatI GCNGC 3 cut(s) 945, 972, 1158
Sau3AI GATC 3 cut(s) 98, 1026, 1377
Sau96I GGNCC 1 cut(s) 1701
SchI GAGTC 2 cut(s) 669, 874
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 2 cut(s) 1544, 1560
SfaNI GCATC 4 cut(s) 331, 958, 989, 1032
SfcI CTRYAG 2 cut(s) 1320, 1462
SmiMI CAYNNNNRTG 5 cut(s) 668, 985, 1037, 1257, 1439
Sse9I AATT 7 cut(s) 87, 923, 975, 1164, 1410, 1524, 1687
SsiI CCGC 2 cut(s) 150, 1325
SspMI CTAG 4 cut(s) 113, 609, 935, 1607
SstI GAGCTC 2 cut(s) 1544, 1560
StyD4I CCNGG 1 cut(s) 225
TaaI ACNGT 8 cut(s) 83, 393, 643, 691, 962, 1003, 1735, 1807
TaiI ACGT 1 cut(s) 1718
TaqI TCGA 4 cut(s) 101, 781, 966, 1737
TasI AATT 7 cut(s) 87, 923, 975, 1164, 1410, 1524, 1687
TatI WGTACW 1 cut(s) 998
Tru1I TTAA 4 cut(s) 156, 1146, 1563, 1677
Tru9I TTAA 4 cut(s) 156, 1146, 1563, 1677
TscAI CASTG 6 cut(s) 88, 525, 922, 1252, 1579, 1810
TseFI GTSAC 2 cut(s) 661, 1250
TseI GCWGC 3 cut(s) 944, 971, 1157
Tsp45I GTSAC 2 cut(s) 661, 1250
TspGWI ACGGA 1 cut(s) 1093
TspRI CASTG 6 cut(s) 88, 525, 922, 1252, 1579, 1810
XagI CCTNNNNNAGG 1 cut(s) 555
XceI RCATGY 2 cut(s) 1080, 1432
XmiI GTMKAC 2 cut(s) 1496, 1583
XspI CTAG 4 cut(s) 113, 609, 935, 1607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.