Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004855

Physical Location & Seq

Genomic Coordinates Forward (+)

27888187 .. 27890493

Gene Structure 2307 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1197700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1653 bp

ATGGCATTCGTCGCAAACATGTGCAGCTTGGTGCTGTACTTTCTCTACATGATGCATTTGGATCTGGCGCACTCCGCCAACCTTCTCACAAACTTCATGGGATCAACTTACCTCCTCTCCCTTGTTGGAGGCTTCATTTCGGACACATACTTGAGCAGATTCACAACCTGCCTGATTTTCGGCGCGGTTGAAATACTGGCTTTGGCGATGACGACAGTTCAAGCTTACTCAAAGCATTTGCACCCAAAAGAAACTTGCCTGACAAAGTGTGTCGAAGGCCATATCGCTGCCATGCTCTATGCTTCTCTGTGCTTGTTAGCACTGGGAACAGGCGGTGTGAGGGGAGCGCTTCCCGCACTTGGTGCTGATCAGTTTAACAGGAAAGAGGAAGCAAAGTCACTCGCAACATATTTCAACTGGTTTATGCTTAGTACTATAGTTGGATCAACTATTGGTGTCACAGCCATTGTGACTGTGAGCACCGAAGTAGCTTGGTACTGGGGATTTTTAATATGCACGGTTGCACCCTTTATCGGGTTCGCTGTTCTTGCTATGGGCAAGCCTTTCTACCGCCTCCAAATTCCCGGCGACAGCCCCATCATTAGAGTCATTCAGGTTATCGTTGTAGCAATAAAAAACAGAAATTTACCGCAGCCTGGGAGCTCTCATCAGCTGTATGAGATCAGTGAAAAGGAATCAAATTATAATGAAGAAAAAATTGCACATACAGATCAATTCAGTTGTCTAGACAAAGCTGCCATACTTGGCAAAGACTCCAAGCCCCAACCATGGAAAGTTTGCACAGTAACACAAGTGGAAGAAGTGAAGGTCCTAACAAGAATGTTGCCAATTCTCTTCAGCACCATCATCATGAACACTTGTTTGGCTCAGCTCCAAACATTTTCAGTCCAACAAGGAAACACCATGGACCGCCACTTAGGCCGCATTGAGGTTCCGGCCCCATCAATCCCCATCATCCCTCTCCTGTTCATGTCCATCCTCATCCCGCTCTACGAGTTCTTCTTCGTCCCCTTCGCGCGAAAAATCACAAAGCACCCATCAGGTATCACGCAGCTTCAGCGCGTCGGCGTTGGACTAGTCCTCTGCGCAATCTCAATGACAATAGCCGGCATAGTAGAAGTGAAGAGAAAGAACCAAGCCAACAAGGACGTCACAAAGCCGATATCCCTCTTTTGGCTTTCTTTCCAATATGGTATTTTCGGCATTGCTGACATGTTTACCGTAGTAGGGTTACTTGAATTCTTCTACAGGGAAGCTCCGGCGGGCATGAAGTCGCTTTCCACTTCATTCACGTGGATTTCGTTTTCTTTCGGGTACTTTTTGAGCTCGATTTTCGTCGCAGTGATCAATAGCGTGACGCGAAGAATTGCACCGAGCAAACTAGGATGGCTGCATGGAAAAGACCTAAACAAAAACAATTTGCATCTTTTTTACTATTTTTTGGCAATCCTTAGCTGCTTCAACTTTGTGAACTATGTCTATTGGGCTTCATGGTACAAGTACAAAGCAGATGAGGCAGATTCTAAGCTGCAGCTTAGGGCTTTGAATCAAACCCCTCTCTGTCACAATGACTCAAGTGTTTTACAAGAAAGTAGAAGCAGGGCTAATGAAGCTGCTGCCTCGATCCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005319 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0008028 GO:0008150 GO:0008509 GO:0008514 GO:0009605 GO:0009607 GO:0009624 GO:0010119 GO:0010876 GO:0015075 GO:0015318 GO:0015665 GO:0015711 GO:0015718 GO:0015849 GO:0015850 GO:0016020 GO:0022857 GO:0033036 GO:0034220 GO:0043207 GO:0044464 GO:0046864 GO:0046865 GO:0046942 GO:0046943 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0065007 GO:0071702 GO:0071944 GO:0080168 GO:0090440 GO:0098656 GO:1901618 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PTR2

Protein Analysis

551

Amino Acids

61.38

Weight (kDa)

8.91

Isoelectric Point (pI)

35.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	16 - 449	1.3e-11	Major Facilitator Superfamily
PTR2	PF00854	54 - 462	2.3e-80	POT family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004855)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g21811 FvH4_4g21812 FvH4_6g08650 FvH4_6g08650
malus_domestica	MD04G1184500.v1.1 MD12G1197600.v1.1 MD12G1197700.v1.1 MD13G1136700.v1.1 MD16G1132000.v1.1
prunus_persica	Prupe.1G208700_v2.0.a1 Prupe.6G303000_v2.0.a1
pyrus_communis	pycom04g16360
rosa_chinensis	RchiOBHm_Chr3g0458781 RchiOBHm_Chr4g0428051
rosa_laevigata	RLG00000007186 RLG00000025135
rosa_multiflora	Rmu_co8474865.1_g000001 Rmu_sc0001634.1_g000029
rosa_roxburghii	Rroxscaffold_5G00369770 Rroxscaffold_6G00420790
rosa_rugosa	Rorug03G0031800
rosa_samantha	Rh3AG089500 Rh3BG092400 Rh3CG092700 Rh3DG093400 Rh4AG279100 Rh4BG284500 Rh4CG300200 Rh4DG281600
rosa_wichuraiana	Rw3G007660 Rw4G024250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	705
AatII	GACGTC	1 cut(s)	1173
Acc16I	TGCGCA	1 cut(s)	1108
Acc36I	ACCTGC	1 cut(s)	176
AccBSI	CCGCTC	1 cut(s)	1009
AccII	CGCG	5 cut(s)	185, 1037, 1039, 1083, 1381
AclWI	GGATC	4 cut(s)	69, 109, 451, 1639
AcsI	RAATTY	3 cut(s)	579, 643, 1259
AcuI	CTGAAG	2 cut(s)	841, 1061
AcvI	CACGTG	1 cut(s)	1314
AcyI	GRCGYC	1 cut(s)	1170
AdeI	CACNNNGTG	1 cut(s)	362
AfaI	GTAC	6 cut(s)	38, 433, 497, 1337, 1517, 1523
AfeI	AGCGCT	1 cut(s)	348
AfiI	CCNNNNNNNGG	8 cut(s)	359, 533, 534, 656, 789, 949, 1194, 1248
AflIII	ACRYGT	2 cut(s)	18, 1233
AgsI	TTSAA	6 cut(s)	191, 221, 415, 1259, 1483, 1567
AhlI	ACTAGT	1 cut(s)	1096
AjnI	CCWGG	1 cut(s)	655
AjuI	GAANNNNNNNTTGG	2 cut(s)	866, 898
Alw21I	GWGCWC	3 cut(s)	482, 665, 1349
AlwI	GGATC	4 cut(s)	69, 109, 451, 1639
Aor51HI	AGCGCT	1 cut(s)	348
AoxI	GGCC	3 cut(s)	277, 940, 957
ApoI	RAATTY	3 cut(s)	579, 643, 1259
AspLEI	GCGC	6 cut(s)	70, 185, 349, 1039, 1083, 1109
AspS9I	GGNCC	3 cut(s)	829, 928, 958
AsuC2I	CCSGG	1 cut(s)	585
AvaII	GGWCC	2 cut(s)	829, 928
BanII	GRGCYC	2 cut(s)	665, 1349
BbrPI	CACGTG	1 cut(s)	1314
Bbv12I	GWGCWC	3 cut(s)	482, 665, 1349
BccI	CCATC	7 cut(s)	605, 872, 970, 980, 1004, 1066, 1401
BciT130I	CCWGG	1 cut(s)	657
BclI	TGATCA	2 cut(s)	367, 1365
BcnI	CCSGG	1 cut(s)	585
BcuI	ACTAGT	1 cut(s)	1096
BfaI	CTAG	3 cut(s)	746, 1097, 1403
BfmI	CTRYAG	3 cut(s)	435, 1267, 1550
BfoI	RGCGCY	1 cut(s)	350
BfuAI	ACCTGC	1 cut(s)	176
BglI	GCCNNNNNGGC	1 cut(s)	939
BlpI	GCTNAGC	1 cut(s)	888
BmcAI	AGTACT	1 cut(s)	433
Bme1390I	CCNGG	2 cut(s)	585, 657
Bme18I	GGWCC	2 cut(s)	829, 928
BmgT120I	GGNCC	3 cut(s)	829, 928, 958
BmiI	GGNNCC	2 cut(s)	954, 960
BmrFI	CCNGG	2 cut(s)	585, 657
BmrI	ACTGGG	2 cut(s)	332, 508
BmsI	GCATC	2 cut(s)	42, 1453
BmuI	ACTGGG	2 cut(s)	332, 508
Bpu10I	CCTNAGC	2 cut(s)	1472, 1556
Bpu1102I	GCTNAGC	1 cut(s)	888
BpuEI	CTTGAG	2 cut(s)	172, 1579
BpuMI	CCSGG	1 cut(s)	585
BsaAI	YACGTR	1 cut(s)	1314
BsaHI	GRCGYC	1 cut(s)	1170
BsaJI	CCNNGG	3 cut(s)	656, 788, 924
BsaXI	ACNNNNNCTCC	2 cut(s)	101, 131
Bsc4I	CCNNNNNNNGG	8 cut(s)	359, 533, 534, 656, 789, 949, 1194, 1248
Bse118I	RCCGGY	1 cut(s)	1127
Bse1I	ACTGG	4 cut(s)	201, 327, 422, 503
Bse3DI	GCAATG	1 cut(s)	1224
BseBI	CCWGG	1 cut(s)	657
BseDI	CCNNGG	3 cut(s)	656, 788, 924
BseGI	GGATG	4 cut(s)	975, 996, 1002, 1412
BseLI	CCNNNNNNNGG	8 cut(s)	359, 533, 534, 656, 789, 949, 1194, 1248
BseMI	GCAATG	1 cut(s)	1224
BseMII	CTCAG	1 cut(s)	902
BseNI	ACTGG	4 cut(s)	201, 327, 422, 503
BseRI	GAGGAG	1 cut(s)	104
BsgI	GTGCAG	1 cut(s)	43
Bsh1236I	CGCG	5 cut(s)	185, 1037, 1039, 1083, 1381
BshFI	GGCC	3 cut(s)	279, 942, 959
BsiHKAI	GWGCWC	3 cut(s)	482, 665, 1349
BsiSI	CCGG	4 cut(s)	585, 956, 1128, 1280
BslFI	GGGAC	1 cut(s)	1013
BslI	CCNNNNNNNGG	8 cut(s)	359, 533, 534, 656, 789, 949, 1194, 1248
BsmFI	GGGAC	1 cut(s)	1013
BsmI	GAATGC	1 cut(s)	5
BsnI	GGCC	3 cut(s)	279, 942, 959
Bsp1286I	GDGCHC	3 cut(s)	482, 665, 1349
Bsp143I	GATC	8 cut(s)	61, 101, 367, 443, 681, 730, 1365, 1644
Bsp1720I	GCTNAGC	1 cut(s)	888
Bsp19I	CCATGG	2 cut(s)	788, 924
BspANI	GGCC	3 cut(s)	279, 942, 959
BspCNI	CTCAG	1 cut(s)	901
BspFNI	CGCG	5 cut(s)	185, 1037, 1039, 1083, 1381
BspHI	TCATGA	1 cut(s)	870
BspLI	GGNNCC	2 cut(s)	954, 960
BspMAI	CTGCAG	1 cut(s)	1554
BspMI	ACCTGC	1 cut(s)	176
BspPI	GGATC	4 cut(s)	69, 109, 451, 1639
BsrBI	CCGCTC	1 cut(s)	1009
BsrDI	GCAATG	1 cut(s)	1224
BsrFI	RCCGGY	1 cut(s)	1127
BsrI	ACTGG	4 cut(s)	201, 327, 422, 503
BssAI	RCCGGY	1 cut(s)	1127
BssECI	CCNNGG	3 cut(s)	656, 788, 924
BssMI	GATC	8 cut(s)	61, 101, 367, 443, 681, 730, 1365, 1644
BssNI	GRCGYC	1 cut(s)	1170
BssT1I	CCWWGG	2 cut(s)	788, 924
Bst2UI	CCWGG	1 cut(s)	657
Bst4CI	ACNGT	5 cut(s)	217, 475, 520, 805, 1243
Bst6I	CTCTTC	2 cut(s)	860, 1139
BstACI	GRCGYC	1 cut(s)	1170
BstAPI	GCANNNNNTGC	1 cut(s)	362
BstBAI	YACGTR	1 cut(s)	1314
BstC8I	GCNNGC	3 cut(s)	560, 1129, 1285
BstDEI	CTNAG	6 cut(s)	428, 888, 937, 1472, 1545, 1556
BstDSI	CCRYGG	2 cut(s)	788, 924
BstF5I	GGATG	4 cut(s)	975, 996, 1002, 1412
BstFNI	CGCG	5 cut(s)	185, 1037, 1039, 1083, 1381
BstH2I	RGCGCY	1 cut(s)	350
BstHHI	GCGC	6 cut(s)	70, 185, 349, 1039, 1083, 1109
BstKTI	GATC	8 cut(s)	64, 104, 370, 446, 684, 733, 1368, 1647
BstMBI	GATC	8 cut(s)	61, 101, 367, 443, 681, 730, 1365, 1644
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 74, 353, 362, 548, 939, 1078, 1535, 1631
BstNI	CCWGG	1 cut(s)	657
BstNSI	RCATGY	2 cut(s)	22, 1237
BstSCI	CCNGG	2 cut(s)	583, 655
BstSFI	CTRYAG	3 cut(s)	435, 1267, 1550
BstUI	CGCG	5 cut(s)	185, 1037, 1039, 1083, 1381
BstX2I	RGATCY	1 cut(s)	61
BstYI	RGATCY	1 cut(s)	61
BsuRI	GGCC	3 cut(s)	279, 942, 959
BtgI	CCRYGG	2 cut(s)	788, 924
BtgZI	GCGATG	1 cut(s)	221
BtsCI	GGATG	4 cut(s)	975, 996, 1002, 1412
BtsI	GCAGTG	1 cut(s)	1368
BtsIMutI	CAGTG	4 cut(s)	320, 691, 1368, 1648
BveI	ACCTGC	1 cut(s)	176
Cac8I	GCNNGC	3 cut(s)	560, 1129, 1285
CciI	TCATGA	1 cut(s)	870
CfoI	GCGC	6 cut(s)	70, 185, 349, 1039, 1083, 1109
Cfr10I	RCCGGY	1 cut(s)	1127
Cfr13I	GGNCC	3 cut(s)	829, 928, 958
CseI	GACGC	2 cut(s)	1072, 1387
Csp6I	GTAC	6 cut(s)	37, 432, 496, 1336, 1516, 1522
CviQI	GTAC	6 cut(s)	37, 432, 496, 1336, 1516, 1522
DdeI	CTNAG	6 cut(s)	428, 888, 937, 1472, 1545, 1556
DpnI	GATC	8 cut(s)	63, 103, 369, 445, 683, 732, 1367, 1646
DpnII	GATC	8 cut(s)	61, 101, 367, 443, 681, 730, 1365, 1644
DraIII	CACNNNGTG	1 cut(s)	362
Eam1104I	CTCTTC	2 cut(s)	860, 1139
EarI	CTCTTC	2 cut(s)	860, 1139
EciI	GGCGGA	1 cut(s)	64
Ecl136II	GAGCTC	2 cut(s)	663, 1347
Eco130I	CCWWGG	2 cut(s)	788, 924
Eco24I	GRGCYC	2 cut(s)	665, 1349
Eco32I	GATATC	1 cut(s)	1185
Eco47I	GGWCC	2 cut(s)	829, 928
Eco47III	AGCGCT	1 cut(s)	348
Eco53kI	GAGCTC	2 cut(s)	663, 1347
Eco57I	CTGAAG	2 cut(s)	841, 1061
Eco72I	CACGTG	1 cut(s)	1314
EcoICRI	GAGCTC	2 cut(s)	663, 1347
EcoO109I	RGGNCCY	1 cut(s)	829
EcoRI	GAATTC	1 cut(s)	1259
EcoRII	CCWGG	1 cut(s)	655
EcoRV	GATATC	1 cut(s)	1185
EcoT14I	CCWWGG	2 cut(s)	788, 924
EcoT22I	ATGCAT	1 cut(s)	57
EcoT38I	GRGCYC	2 cut(s)	665, 1349
ErhI	CCWWGG	2 cut(s)	788, 924
FaqI	GGGAC	1 cut(s)	1013
FauI	CCCGC	3 cut(s)	361, 1014, 1276
FbaI	TGATCA	2 cut(s)	367, 1365
FokI	GGATG	4 cut(s)	962, 983, 989, 1419
FriOI	GRGCYC	2 cut(s)	665, 1349
FspBI	CTAG	3 cut(s)	746, 1097, 1403
FspI	TGCGCA	1 cut(s)	1108
GlaI	GCGC	6 cut(s)	69, 184, 348, 1038, 1082, 1108
HaeII	RGCGCY	1 cut(s)	350
HaeIII	GGCC	3 cut(s)	279, 942, 959
HapII	CCGG	4 cut(s)	585, 956, 1128, 1280
HgaI	GACGC	2 cut(s)	1072, 1387
HhaI	GCGC	6 cut(s)	70, 185, 349, 1039, 1083, 1109
Hin1I	GRCGYC	1 cut(s)	1170
Hin6I	GCGC	6 cut(s)	68, 183, 347, 1037, 1081, 1107
HinP1I	GCGC	6 cut(s)	68, 183, 347, 1037, 1081, 1107
HindIII	AAGCTT	1 cut(s)	222
HinfI	GANTC	7 cut(s)	159, 606, 695, 773, 1541, 1567, 1592
HpaII	CCGG	4 cut(s)	585, 956, 1128, 1280
Hpy166II	GTNNAC	2 cut(s)	1239, 1492
Hpy188I	TCNGA	1 cut(s)	142
Hpy188III	TCNNGA	2 cut(s)	746, 871
Hpy8I	GTNNAC	2 cut(s)	1239, 1492
Hpy99I	CGWCG	3 cut(s)	14, 1088, 1361
HpyAV	CCTTC	4 cut(s)	92, 269, 820, 1042
HpyCH4III	ACNGT	5 cut(s)	217, 475, 520, 805, 1243
HpyCH4IV	ACGT	2 cut(s)	1170, 1313
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 74, 353, 362, 548, 939, 1078, 1535, 1631
HpyF3I	CTNAG	6 cut(s)	428, 888, 937, 1472, 1545, 1556
HpySE526I	ACGT	2 cut(s)	1170, 1313
Hsp92I	GRCGYC	1 cut(s)	1170
HspAI	GCGC	6 cut(s)	68, 183, 347, 1037, 1081, 1107
KroI	GCCGGC	1 cut(s)	1127
KroNI	GCCGGC	1 cut(s)	1129
Ksp22I	TGATCA	2 cut(s)	367, 1365
Kzo9I	GATC	8 cut(s)	61, 101, 367, 443, 681, 730, 1365, 1644
LmnI	GCTCC	4 cut(s)	344, 660, 897, 1282
LweI	GCATC	2 cut(s)	42, 1453
MaeI	CTAG	3 cut(s)	746, 1097, 1403
MaeII	ACGT	2 cut(s)	1170, 1313
MaeIII	GTNAC	8 cut(s)	396, 457, 469, 805, 1171, 1251, 1375, 1583
MalI	GATC	8 cut(s)	63, 103, 369, 445, 683, 732, 1367, 1646
MbiI	CCGCTC	1 cut(s)	1009
MboI	GATC	8 cut(s)	61, 101, 367, 443, 681, 730, 1365, 1644
MboII	GAAGA	8 cut(s)	722, 830, 847, 1012, 1015, 1156, 1255, 1395
MflI	RGATCY	1 cut(s)	61
MhlI	GDGCHC	3 cut(s)	482, 665, 1349
MluCI	AATT	9 cut(s)	579, 643, 700, 717, 734, 849, 1259, 1386, 1438
MlyI	GAGTC	3 cut(s)	615, 767, 1586
MmeI	TCCRAC	4 cut(s)	106, 421, 934, 1072
Mph1103I	ATGCAT	1 cut(s)	57
MroNI	GCCGGC	1 cut(s)	1127
MseI	TTAA	2 cut(s)	375, 509
MslI	CAYNNNNRTG	2 cut(s)	869, 1312
MspA1I	CMGCKG	1 cut(s)	673
MspI	CCGG	4 cut(s)	585, 956, 1128, 1280
MspR9I	CCNGG	2 cut(s)	585, 657
Mva1269I	GAATGC	1 cut(s)	5
MvaI	CCWGG	1 cut(s)	657
MvnI	CGCG	5 cut(s)	185, 1037, 1039, 1083, 1381
MwoI	GCNNNNNNNGC	9 cut(s)	11, 74, 353, 362, 548, 939, 1078, 1535, 1631
NaeI	GCCGGC	1 cut(s)	1129
NciI	CCSGG	1 cut(s)	585
NcoI	CCATGG	2 cut(s)	788, 924
NdeII	GATC	8 cut(s)	61, 101, 367, 443, 681, 730, 1365, 1644
NgoMIV	GCCGGC	1 cut(s)	1127
NlaIV	GGNNCC	2 cut(s)	954, 960
NmuCI	GTSAC	6 cut(s)	396, 457, 469, 1171, 1375, 1583
NsbI	TGCGCA	1 cut(s)	1108
NsiI	ATGCAT	1 cut(s)	57
NspI	RCATGY	2 cut(s)	22, 1237
PagI	TCATGA	1 cut(s)	870
PciI	ACATGT	2 cut(s)	18, 1233
PcsI	WCGNNNNNNNCGW	1 cut(s)	1319
PctI	GAATGC	1 cut(s)	5
PdiI	GCCGGC	1 cut(s)	1129
PfeI	GAWTC	4 cut(s)	159, 695, 1541, 1567
PleI	GAGTC	3 cut(s)	614, 767, 1586
PmaCI	CACGTG	1 cut(s)	1314
PmlI	CACGTG	1 cut(s)	1314
PpsI	GAGTC	3 cut(s)	614, 767, 1586
Ppu21I	YACGTR	1 cut(s)	1314
PpuMI	RGGWCCY	1 cut(s)	829
PscI	ACATGT	2 cut(s)	18, 1233
PsiI	TTATAA	1 cut(s)	705
Psp124BI	GAGCTC	2 cut(s)	665, 1349
Psp5II	RGGWCCY	1 cut(s)	829
Psp6I	CCWGG	1 cut(s)	655
PspCI	CACGTG	1 cut(s)	1314
PspGI	CCWGG	1 cut(s)	655
PspN4I	GGNNCC	2 cut(s)	954, 960
PspPI	GGNCC	3 cut(s)	829, 928, 958
PspPPI	RGGWCCY	1 cut(s)	829
PstI	CTGCAG	1 cut(s)	1554
PsuI	RGATCY	1 cut(s)	61
PvuII	CAGCTG	1 cut(s)	673
RsaI	GTAC	6 cut(s)	38, 433, 497, 1337, 1517, 1523
RsaNI	GTAC	6 cut(s)	37, 432, 496, 1336, 1516, 1522
RseI	CAYNNNNRTG	2 cut(s)	869, 1312
SacI	GAGCTC	2 cut(s)	665, 1349
SaqAI	TTAA	2 cut(s)	375, 509
Sau3AI	GATC	8 cut(s)	61, 101, 367, 443, 681, 730, 1365, 1644
Sau96I	GGNCC	3 cut(s)	829, 928, 958
ScaI	AGTACT	1 cut(s)	433
SchI	GAGTC	3 cut(s)	615, 767, 1586
ScrFI	CCNGG	2 cut(s)	585, 657
SduI	GDGCHC	3 cut(s)	482, 665, 1349
SfaNI	GCATC	2 cut(s)	42, 1453
SfcI	CTRYAG	3 cut(s)	435, 1267, 1550
SinI	GGWCC	2 cut(s)	829, 928
SmiMI	CAYNNNNRTG	2 cut(s)	869, 1312
SmlI	CTYRAG	2 cut(s)	151, 1594
SmoI	CTYRAG	2 cut(s)	151, 1594
SpeI	ACTAGT	1 cut(s)	1096
Sse9I	AATT	9 cut(s)	579, 643, 700, 717, 734, 849, 1259, 1386, 1438
SspMI	CTAG	3 cut(s)	746, 1097, 1403
SstI	GAGCTC	2 cut(s)	665, 1349
StyD4I	CCNGG	2 cut(s)	583, 655
StyI	CCWWGG	2 cut(s)	788, 924
TaaI	ACNGT	5 cut(s)	217, 475, 520, 805, 1243
TaiI	ACGT	2 cut(s)	1173, 1316
TaqI	TCGA	3 cut(s)	273, 1349, 1643
TasI	AATT	9 cut(s)	579, 643, 700, 717, 734, 849, 1259, 1386, 1438
TatI	WGTACW	3 cut(s)	36, 431, 1521
TauI	GCSGC	1 cut(s)	945
TfiI	GAWTC	4 cut(s)	159, 695, 1541, 1567
Tru1I	TTAA	2 cut(s)	375, 509
Tru9I	TTAA	2 cut(s)	375, 509
TscAI	CASTG	3 cut(s)	327, 691, 1368
TseFI	GTSAC	6 cut(s)	396, 457, 469, 1171, 1375, 1583
Tsp45I	GTSAC	6 cut(s)	396, 457, 469, 1171, 1375, 1583
TspDTI	ATGAA	9 cut(s)	85, 124, 723, 887, 979, 1296, 1304, 1500, 1644
TspRI	CASTG	3 cut(s)	327, 691, 1368
VpaK11BI	GGWCC	2 cut(s)	829, 928
XapI	RAATTY	3 cut(s)	579, 643, 1259
XbaI	TCTAGA	1 cut(s)	745
XceI	RCATGY	2 cut(s)	22, 1237
XspI	CTAG	3 cut(s)	746, 1097, 1403
ZraI	GACGTC	1 cut(s)	1171
ZrmI	AGTACT	1 cut(s)	433
Zsp2I	ATGCAT	1 cut(s)	57

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.