Rh4DG281600

Protein NRT1 PTR FAMILY 4.5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004855
Physical Location & Seq
Reverse (-)
50390806 .. 50393302
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG281600.1

Sequence Viewer

Length: 1752 bp
ATGGGGTTGGACAGTGAAATTGGAAATAGATCGAACAAAGAGCTAGCTTATGAATCTATGAAGAAACAAGAAAGAGAAGGCGGATTTAAGGCCAACTATTTCATCTTTGCAATGATGTTTCTGGATAACATAGGGTTTGTGGCTAACATGGCAAGCCTGGTTCTATACTTTATGCTTGTGATACACTTTGACATCTCTGGCTCTGCTACCACCACAACAAACTACTTGGGTTCTGCATTCTTGCTTACATTAGTTGGAGGATTCATCTCTGATGCCTACATGAATCGCCTCAACACCACTCTACTCTTCGGTGTGGCAGAACTGTTGGGATATTCATTGTTGATTATTCAATCCCATTATACAAAACTGCAACCTTTACCATGTGGAGAATCAACCTGCGTTCATGGCACAAAAGCCTTGCTGTTTTATGCTTCAATATCCTTAGTTGCACTGGGAGGAGGTGGAATCAGAGGAGCGATTCCTGCTTTAGGTGCTGACCAGTTCAACTACAAGAAACCCGAAGAGCGCAAGCATATAGCTAGCTTTTTCAACTGGCTTTTGCTTAGTATAACCGTTGGGGCTGTGGTTGGAGTCACATTTGTGGTATATGTCAGCACAACGGTTGGATGGGACATAGCATTTATCATCTCCTTGTCTTGTGCTGGTGCTGGTCTTGTCATTGTTGCTTTGGGAAAGCCATATTATCGTGTTCGAGTTGCAGGAGAAAGTCCTTTACTAAGGGTTTTACAGGTTTTGGTGGCAAGTGTAAAGAACTGGAGGGTTCAGGTGCCTCAGAGTCCTGATGAACTACATGAATCCACTAAAAAGAGAGAGAGAATCACCCACACTGACCAATTCAGATTACTAGACAAGGCTGCCATTCTCCCTAACGGTGTCGATGCTGCAATTGCATCAAAATGGAAAGTTTGTACAGTAACACAGGTGGAGGAAGTGAAGATCCTCACAAGGATGATGCCTATTCTTCTAAGCACAACCCTCATGAACACATGCTTGGCACAACTGCAAACTCTTTCCGTCCAACAAGGAACCCTCATGGACACACGCATTGGTAGTTTTAATGTTCCAGCAGCCACAATTCCAGTCATTCCTCTACTATTCATGTCTGTTCTTATCCCCACCTACGAGTTCACTTTCGTTCCCCTTTTGCGCAAGTTCACTGGTCACCCCAATGGCATAACCCATCTCCAGAGAGTTGGTGTTGGGCTTGTTCTGTCAGCTATCTCCATGACTATAGCGGGGTTCGTGGAAGTGAAGAGAAAGCATGAGTTCATTCACCACAATCACAAGATCAGCCTCTTTTGGCTCTCATTTCACTATGCAATTTTCGGCATTGCAGACATGTTCACACTTGTGGGGTTGATGGAGTTCTTCTATAGAGAAGCTCCTGCTGGCATGAGGTCTCTGTCTACTTCATTTTCTTGGCTTTCTCTCTCAATTGGGTACTACCTGAGCTCAGCTTTTGTAGAGCTCATTAACTCTATCACTGGCAAGTATACAGACAGCAAGTTAGGGTGGCTAGAGGGGCTTGACATGAACAAGAACCACATAGAACTTTTCTACTGGTTTTTGGCGATTCTGAGCGTGCTTAACTTTGCAAATTATCTTTTCTGGGCCAACTGGTACACGTATAAGAACGATGTTACAGTCGATGAGGAAATGCTAATCAAAGCAGGCCAAGTTGGAGCTGATGAATCTAACTCACTAAGTGTAAGCACTGTGTCCAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

583

Amino Acids

64.74

Weight (kDa)

8.38

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 98 - 504 1.7e-79 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1169
Acc36I ACCTGC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 787
AccI GTMKAC 2 cut(s) 1427, 1514
AciI CCGC 2 cut(s) 81, 1256
AclWI GGATC 1 cut(s) 952
AdeI CACNNNGTG 1 cut(s) 1727
AfaI GTAC 3 cut(s) 931, 1463, 1643
AfiI CCNNNNNNNGG 1 cut(s) 488
AflIII ACRYGT 2 cut(s) 1359, 1644
AgsI TTSAA 4 cut(s) 350, 435, 505, 550
AjnI CCWGG 1 cut(s) 156
AjuI GAANNNNNNNTTGG 2 cut(s) 995, 1027
AleI CACNNNNGTG 2 cut(s) 599, 1370
Alw21I GWGCWC 2 cut(s) 1475, 1491
Alw26I GTCTC 1 cut(s) 1425
AlwI GGATC 1 cut(s) 952
AoxI GGCC 3 cut(s) 90, 1632, 1693
ApeKI GCWGC 3 cut(s) 875, 902, 1088
AspLEI GCGC 2 cut(s) 528, 1170
AspS9I GGNCC 1 cut(s) 1632
AsuHPI GGTGA 3 cut(s) 832, 1175, 1286
AsuNHI GCTAGC 2 cut(s) 43, 539
BanI GGYRCC 1 cut(s) 787
BanII GRGCYC 2 cut(s) 1475, 1491
Bbv12I GWGCWC 2 cut(s) 1475, 1491
BbvI GCAGC 3 cut(s) 862, 889, 1100
BccI CCATC 3 cut(s) 621, 1209, 1375
BciT130I CCWGG 1 cut(s) 158
BcoDI GTCTC 1 cut(s) 1425
BfaI CTAG 4 cut(s) 44, 540, 866, 1538
BfmI CTRYAG 2 cut(s) 1251, 1393
BfuAI ACCTGC 1 cut(s) 404
BisI GCNGC 3 cut(s) 876, 903, 1089
BlpI GCTNAGC 1 cut(s) 1474
BlsI GCNGC 3 cut(s) 877, 904, 1090
Bme1390I CCNGG 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 1632
BmiI GGNNCC 2 cut(s) 789, 1048
BmrFI CCNGG 1 cut(s) 158
BmrI ACTGGG 1 cut(s) 461
BmsI GCATC 4 cut(s) 262, 889, 920, 963
BmtI GCTAGC 2 cut(s) 47, 543
BmuI ACTGGG 1 cut(s) 461
BpmI CTGGAG 2 cut(s) 796, 1190
Bpu10I CCTNAGC 1 cut(s) 1469
Bpu1102I GCTNAGC 1 cut(s) 1474
BsaAI YACGTR 1 cut(s) 1647
BsaI GGTCTC 1 cut(s) 1425
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse1I ACTGG 9 cut(s) 456, 499, 557, 779, 1100, 1183, 1510, 1586, 1643
Bse3DI GCAATG 2 cut(s) 117, 1350
BseBI CCWGG 1 cut(s) 158
BseGI GGATG 2 cut(s) 632, 975
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMI GCAATG 2 cut(s) 117, 1350
BseMII CTCAG 4 cut(s) 806, 1460, 1488, 1589
BseNI ACTGG 9 cut(s) 456, 499, 557, 779, 1100, 1183, 1510, 1586, 1643
BseRI GAGGAG 2 cut(s) 471, 486
BseXI GCAGC 3 cut(s) 862, 889, 1100
BshFI GGCC 3 cut(s) 92, 1634, 1695
BshNI GGYRCC 1 cut(s) 787
BsiHKAI GWGCWC 2 cut(s) 1475, 1491
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 1 cut(s) 1425
BsmFI GGGAC 1 cut(s) 644
BsmI GAATGC 1 cut(s) 236
BsnI GGCC 3 cut(s) 92, 1634, 1695
Bso31I GGTCTC 1 cut(s) 1425
Bsp1286I GDGCHC 2 cut(s) 1475, 1491
Bsp1407I TGTACA 1 cut(s) 929
Bsp143I GATC 3 cut(s) 29, 957, 1308
Bsp1720I GCTNAGC 1 cut(s) 1474
BspACI CCGC 2 cut(s) 81, 1256
BspANI GGCC 3 cut(s) 92, 1634, 1695
BspCNI CTCAG 4 cut(s) 805, 1461, 1487, 1590
BspHI TCATGA 1 cut(s) 999
BspLI GGNNCC 2 cut(s) 789, 1048
BspMI ACCTGC 1 cut(s) 404
BspOI GCTAGC 2 cut(s) 47, 543
BspPI GGATC 1 cut(s) 952
BspQI GCTCTTC 1 cut(s) 516
BspT107I GGYRCC 1 cut(s) 787
BspTNI GGTCTC 1 cut(s) 1425
BsrDI GCAATG 2 cut(s) 117, 1350
BsrGI TGTACA 1 cut(s) 929
BsrI ACTGG 9 cut(s) 456, 499, 557, 779, 1100, 1183, 1510, 1586, 1643
BssMI GATC 3 cut(s) 29, 957, 1308
BssNAI GTATAC 1 cut(s) 1515
Bst1107I GTATAC 1 cut(s) 1515
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 8 cut(s) 14, 324, 574, 622, 893, 934, 1666, 1738
Bst6I CTCTTC 3 cut(s) 311, 516, 1268
BstAUI TGTACA 1 cut(s) 929
BstBAI YACGTR 1 cut(s) 1647
BstC8I GCNNGC 7 cut(s) 45, 154, 530, 541, 1411, 1604, 1693
BstDEI CTNAG 9 cut(s) 442, 563, 737, 792, 986, 1469, 1474, 1598, 1724
BstEII GGTNACC 1 cut(s) 1181
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 2 cut(s) 632, 975
BstHHI GCGC 2 cut(s) 528, 1170
BstKTI GATC 3 cut(s) 32, 960, 1311
BstMAI GTCTC 1 cut(s) 1425
BstMBI GATC 3 cut(s) 29, 957, 1308
BstMWI GCNNNNNNNGC 6 cut(s) 149, 405, 482, 491, 908, 1543
BstNI CCWGG 1 cut(s) 158
BstNSI RCATGY 2 cut(s) 1011, 1363
BstPI GGTNACC 1 cut(s) 1181
BstSCI CCNGG 1 cut(s) 156
BstSFI CTRYAG 2 cut(s) 1251, 1393
BstV1I GCAGC 3 cut(s) 862, 889, 1100
BstX2I RGATCY 1 cut(s) 957
BstXI CCANNNNNNTGG 1 cut(s) 1214
BstYI RGATCY 1 cut(s) 957
BstZ17I GTATAC 1 cut(s) 1515
BsuRI GGCC 3 cut(s) 92, 1634, 1695
BtsCI GGATG 2 cut(s) 632, 975
BtsIMutI CAGTG 6 cut(s) 19, 449, 846, 1176, 1503, 1734
BveI ACCTGC 1 cut(s) 404
Cac8I GCNNGC 7 cut(s) 45, 154, 530, 541, 1411, 1604, 1693
CciI TCATGA 1 cut(s) 999
CfoI GCGC 2 cut(s) 528, 1170
Cfr13I GGNCC 1 cut(s) 1632
Csp6I GTAC 3 cut(s) 930, 1462, 1642
CviQI GTAC 3 cut(s) 930, 1462, 1642
DdeI CTNAG 9 cut(s) 442, 563, 737, 792, 986, 1469, 1474, 1598, 1724
DpnI GATC 3 cut(s) 31, 959, 1310
DpnII GATC 3 cut(s) 29, 957, 1308
DraIII CACNNNGTG 1 cut(s) 1727
Eam1104I CTCTTC 3 cut(s) 311, 516, 1268
EarI CTCTTC 3 cut(s) 311, 516, 1268
EciI GGCGGA 1 cut(s) 96
Ecl136II GAGCTC 2 cut(s) 1473, 1489
Eco24I GRGCYC 2 cut(s) 1475, 1491
Eco31I GGTCTC 1 cut(s) 1425
Eco53kI GAGCTC 2 cut(s) 1473, 1489
Eco91I GGTNACC 1 cut(s) 1181
EcoICRI GAGCTC 2 cut(s) 1473, 1489
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoO65I GGTNACC 1 cut(s) 1181
EcoRII CCWGG 1 cut(s) 156
EcoT38I GRGCYC 2 cut(s) 1475, 1491
FaqI GGGAC 1 cut(s) 644
FauI CCCGC 1 cut(s) 1249
FblI GTMKAC 2 cut(s) 1427, 1514
Fnu4HI GCNGC 3 cut(s) 876, 903, 1089
FokI GGATG 2 cut(s) 639, 982
FriOI GRGCYC 2 cut(s) 1475, 1491
Fsp4HI GCNGC 3 cut(s) 876, 903, 1089
FspBI CTAG 4 cut(s) 44, 540, 866, 1538
FspI TGCGCA 1 cut(s) 1169
GlaI GCGC 2 cut(s) 527, 1169
GluI GCNGC 3 cut(s) 876, 903, 1089
GsuI CTGGAG 2 cut(s) 796, 1190
HaeIII GGCC 3 cut(s) 92, 1634, 1695
HhaI GCGC 2 cut(s) 528, 1170
Hin6I GCGC 2 cut(s) 526, 1168
HinP1I GCGC 2 cut(s) 526, 1168
HphI GGTGA 3 cut(s) 832, 1175, 1286
Hpy166II GTNNAC 6 cut(s) 1149, 1176, 1365, 1428, 1515, 1644
Hpy188I TCNGA 5 cut(s) 271, 470, 795, 860, 1599
Hpy188III TCNNGA 5 cut(s) 122, 800, 1000, 1207, 1743
Hpy8I GTNNAC 6 cut(s) 1149, 1176, 1365, 1428, 1515, 1644
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 8 cut(s) 14, 324, 574, 622, 893, 934, 1666, 1738
HpyCH4IV ACGT 1 cut(s) 1646
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 405, 482, 491, 908, 1543
HpyF3I CTNAG 9 cut(s) 442, 563, 737, 792, 986, 1469, 1474, 1598, 1724
HpySE526I ACGT 1 cut(s) 1646
HspAI GCGC 2 cut(s) 526, 1168
Kzo9I GATC 3 cut(s) 29, 957, 1308
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 3 cut(s) 473, 1408, 1703
Lsp1109I GCAGC 3 cut(s) 862, 889, 1100
LweI GCATC 4 cut(s) 262, 889, 920, 963
MaeI CTAG 4 cut(s) 44, 540, 866, 1538
MaeII ACGT 1 cut(s) 1646
MaeIII GTNAC 4 cut(s) 592, 934, 1181, 1660
MalI GATC 3 cut(s) 31, 959, 1310
MboI GATC 3 cut(s) 29, 957, 1308
MboII GAAGA 7 cut(s) 73, 298, 533, 967, 974, 1285, 1381
MfeI CAATTG 2 cut(s) 906, 1455
MflI RGATCY 1 cut(s) 957
MhlI GDGCHC 2 cut(s) 1475, 1491
MluCI AATT 7 cut(s) 18, 854, 906, 1095, 1341, 1455, 1618
MlyI GAGTC 2 cut(s) 600, 805
MmeI TCCRAC 5 cut(s) 235, 568, 604, 1063, 1681
MseI TTAA 4 cut(s) 87, 1077, 1494, 1608
MslI CAYNNNNRTG 5 cut(s) 599, 916, 968, 1188, 1370
MspR9I CCNGG 1 cut(s) 158
MunI CAATTG 2 cut(s) 906, 1455
Mva1269I GAATGC 1 cut(s) 236
MvaI CCWGG 1 cut(s) 158
MwoI GCNNNNNNNGC 6 cut(s) 149, 405, 482, 491, 908, 1543
NdeII GATC 3 cut(s) 29, 957, 1308
NheI GCTAGC 2 cut(s) 43, 539
NlaIV GGNNCC 2 cut(s) 789, 1048
NmuCI GTSAC 2 cut(s) 592, 1181
NsbI TGCGCA 1 cut(s) 1169
NspI RCATGY 2 cut(s) 1011, 1363
OliI CACNNNNGTG 2 cut(s) 599, 1370
PagI TCATGA 1 cut(s) 999
PciI ACATGT 1 cut(s) 1359
PciSI GCTCTTC 1 cut(s) 516
PctI GAATGC 1 cut(s) 236
PkrI GCNGC 3 cut(s) 877, 904, 1090
PleI GAGTC 2 cut(s) 599, 804
PpsI GAGTC 2 cut(s) 599, 804
Ppu21I YACGTR 1 cut(s) 1647
PscI ACATGT 1 cut(s) 1359
Psp124BI GAGCTC 2 cut(s) 1475, 1491
Psp6I CCWGG 1 cut(s) 156
PspEI GGTNACC 1 cut(s) 1181
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 789, 1048
PspPI GGNCC 1 cut(s) 1632
PsuI RGATCY 1 cut(s) 957
RsaI GTAC 3 cut(s) 931, 1463, 1643
RsaNI GTAC 3 cut(s) 930, 1462, 1642
RseI CAYNNNNRTG 5 cut(s) 599, 916, 968, 1188, 1370
SacI GAGCTC 2 cut(s) 1475, 1491
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 4 cut(s) 87, 1077, 1494, 1608
SatI GCNGC 3 cut(s) 876, 903, 1089
Sau3AI GATC 3 cut(s) 29, 957, 1308
Sau96I GGNCC 1 cut(s) 1632
SchI GAGTC 2 cut(s) 600, 805
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 2 cut(s) 1475, 1491
SfaNI GCATC 4 cut(s) 262, 889, 920, 963
SfcI CTRYAG 2 cut(s) 1251, 1393
SmiMI CAYNNNNRTG 5 cut(s) 599, 916, 968, 1188, 1370
Sse9I AATT 7 cut(s) 18, 854, 906, 1095, 1341, 1455, 1618
SsiI CCGC 2 cut(s) 81, 1256
SspMI CTAG 4 cut(s) 44, 540, 866, 1538
SstI GAGCTC 2 cut(s) 1475, 1491
StyD4I CCNGG 1 cut(s) 156
TaaI ACNGT 8 cut(s) 14, 324, 574, 622, 893, 934, 1666, 1738
TaiI ACGT 1 cut(s) 1649
TaqI TCGA 4 cut(s) 32, 712, 897, 1668
TasI AATT 7 cut(s) 18, 854, 906, 1095, 1341, 1455, 1618
TatI WGTACW 1 cut(s) 929
Tru1I TTAA 4 cut(s) 87, 1077, 1494, 1608
Tru9I TTAA 4 cut(s) 87, 1077, 1494, 1608
TscAI CASTG 6 cut(s) 19, 456, 853, 1183, 1510, 1741
TseFI GTSAC 2 cut(s) 592, 1181
TseI GCWGC 3 cut(s) 875, 902, 1088
Tsp45I GTSAC 2 cut(s) 592, 1181
TspGWI ACGGA 1 cut(s) 1024
TspRI CASTG 6 cut(s) 19, 456, 853, 1183, 1510, 1741
XagI CCTNNNNNAGG 1 cut(s) 486
XceI RCATGY 2 cut(s) 1011, 1363
XmiI GTMKAC 2 cut(s) 1427, 1514
XspI CTAG 4 cut(s) 44, 540, 866, 1538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.