Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0004855

Physical Location & Seq

Genomic Coordinates Forward (+)

2516048 .. 2521967

Gene Structure 5920 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0031800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2157 bp

ATGGTGGCTCGGAGCTTCCAGGTCCGCCACAACGACTCCGATTTCACCGTCGTCTACGACACCGACGACGGCCTCGAAGTTCTGAAATACCAGCTCTTCTCTCTCACGTCTGTACCTCCTGATGAGCAAAAGATCATCGGAGCCGATGACGACCGCGTCGTTTCGGATGATTCGGACCTGGTTGCGATCTCCGAGAAGCTCCGGTTGGTTTCGATCAGCGAAGAGGAACCGGAATCGACGGCCGGGAATGAGGAATTGCTGAAATCCGATGAGGAATTGGCCAGAATGTTGCAGGCGGAGGAGGAGGCGCTTATGTATCAGCAATACGCTGTTCCTGAAGATAATGGAGCATTTGAGCAAAGAGTGCGGCCTTATGTCAGTCAAGTTCTTCTGTATGAGACCGCGGTACGCCAGGAGGCTGCTAGGAAAACAGTCCCTGTAGAACAGCTTGAGGAGAAGGCATTGGTCAGTTTGGCCAAGGAGGGGAACTTAACTCCATCAAAGAATGAACAAGATCATGCGTTCCTGCTGCAGCTGCTTTTCTGGTTCAAACAATCCTTCAGGTGGGTTAATGAACCTCCCTGTGATGGTTGCGGCAAGAAAACCATCAGTGTTGGCATGGATGTTGCACTTCTTCATCAAGGTTCTCGAGTTGAAGTTTATAGATGCACTTCTTGCCCAGCAGAGACTCGTTTCCCACGCTACAATGATCCACTAAAGCTTCTGGAAACAAGAAGGGGTCGTTGTGGGGAATGGGCAAATTGCTTCACACTTTATTGCCGAGCTTTTGGGTATGAATCACGCCTGATCCTGGATTTCACAGATCATGTTTGGACAGAGTGCTTCTCAGAATCTTTAGGAAGATGGATGCATCTTGATCCTTGTGAAGGAGTGTATGACAAACCACTGTTATATGAAAGCGGGTGGAATAAGAAATTAAATTATGTACTTGCCATTGGGAAGGATGGAGTATGTGATGTAACTAAACGCTACACAAGGAAGTGGCATGAGGTTCTTTCTCGACGTAACATTATTGCAGAGCCTGCACTGTCATCTGTGCTTGCTAATATAACAAAAGAATGTCGGAGAGGCTTTACTTCTCAAGTGCGTTCTGCACTTGAAGACCGCGATGAGAAGGAAAGACAACAACTTGAGAGAGATTTACATTCTACAGATGATGCCTCAACCTCATTACCTGGAAGACGAAGTGGGGACAAGGAATGGCGCAAGTCAAGAATGGAAGATGGCTCTGATGAGAGTTGCTCCTTAACTGGTTCTTCTTGTCCACTTCGTCAATGTGTGGACGAGCATGTGACAAAAATTTACAATGGTTTTTTTCCAATTCTTTCACAGTTGGTTGATGAAGGTTTTCCGAAGTCAAGGGCAGTTGAAGTACTGGAGATTCTTAAAGAAATTCTCATAAATCTTAAGAAATCCCCTTTTAAAACAAGGAGGGTGTCAATAGATTCAGTTCCAAATATCAGCCAGTCTGTTGTTCAGCAGTTGTTGCCCTCTTTCACTGAGTTACTCAGCGCTCTTTCATTGAGTAGCAAGGCAGATACTGATGGGAGGGTTGACATTTATTTGGCTGGACCTCCTGTTAAAACCTCTATGGCACTGCCTGTTACATTCCATGCTTTGGACGTCACGATCCGTAATCTCAAGAGTTGTGAAAACTTTGTTAAAGATTCTCTGTGCTTGCCGCTTCTTAAGTTAAACAGAATACATTCCGGTGTAGTCCGTGCAAGTGGAGAAGAAATTCCTTTTGGAATTGCCACATCAGCTTTTGATGGGACACGCATGTCCAAGTGGGAAGAACCAAATGGTGCAGAAGGTTGTTGGATCATGTATAAAGTATCGGATAACCAGATGCACGAACTGGTGGCATATGAGTTGATGTCAGCCAATGATGCACCTGAAAGAGATCCAATGGATTGGGTTGTCGAGGGAAGCAATGATGGGGGGTCAAGCTGGCATTTATTAGATAAACAAACTTCTCAACTATTCGACAGTCGTTTTCAGTGTAGAACATTTAAAGTTTCATCTGAAGGTTTCCTATCAAATGCTTTCAGGTTTAGATTTTTGAGGGTTCGTGATATTCAATCAAATTCTCGACTGCAATTAGGTAGCATTGATCTCTATTCTAGAAGCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005319 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0008028 GO:0008150 GO:0008509 GO:0008514 GO:0009605 GO:0009607 GO:0009624 GO:0010119 GO:0010876 GO:0015075 GO:0015318 GO:0015665 GO:0015711 GO:0015718 GO:0015849 GO:0015850 GO:0016020 GO:0022857 GO:0033036 GO:0034220 GO:0043207 GO:0044464 GO:0046864 GO:0046865 GO:0046942 GO:0046943 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0065007 GO:0071702 GO:0071944 GO:0080168 GO:0090440 GO:0098656 GO:1901618 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PTR2

Protein Analysis

718

Amino Acids

81.52

Weight (kDa)

5.26

Isoelectric Point (pI)

55.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transglut_core	PF01841	216 - 294	1.6e-16	Transglutaminase-like superfamily
Rad4	PF03835	276 - 384	3e-11	Rad4 transglutaminase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004855)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g21811 FvH4_4g21812 FvH4_6g08650 FvH4_6g08650
malus_domestica	MD04G1184500.v1.1 MD12G1197600.v1.1 MD12G1197700.v1.1 MD13G1136700.v1.1 MD16G1132000.v1.1
prunus_persica	Prupe.1G208700_v2.0.a1 Prupe.6G303000_v2.0.a1
pyrus_communis	pycom04g16360
rosa_chinensis	RchiOBHm_Chr3g0458781 RchiOBHm_Chr4g0428051
rosa_laevigata	RLG00000007186 RLG00000025135
rosa_multiflora	Rmu_co8474865.1_g000001 Rmu_sc0001634.1_g000029
rosa_roxburghii	Rroxscaffold_5G00369770 Rroxscaffold_6G00420790
rosa_rugosa	Rorug03G0031800
rosa_samantha	Rh3AG089500 Rh3BG092400 Rh3CG092700 Rh3DG093400 Rh4AG279100 Rh4BG284500 Rh4CG300200 Rh4DG281600
rosa_wichuraiana	Rw3G007660 Rw4G024250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	155, 1801
AatII	GACGTC	1 cut(s)	1647
AccB7I	CCANNNNNTGG	1 cut(s)	1639
AccI	GTMKAC	1 cut(s)	54
AccII	CGCG	3 cut(s)	156, 404, 1128
AclWI	GGATC	6 cut(s)	704, 802, 872, 1645, 1850, 1919
AcoI	YGGCCR	3 cut(s)	240, 279, 474
AcsI	RAATTY	4 cut(s)	1320, 1413, 1758, 2107
AcuI	CTGAAG	3 cut(s)	357, 544, 2067
AcyI	GRCGYC	1 cut(s)	1644
AfaI	GTAC	4 cut(s)	114, 408, 948, 1395
AfeI	AGCGCT	1 cut(s)	1534
AfiI	CCNNNNNNNGG	6 cut(s)	483, 564, 587, 811, 887, 1639
AflII	CTTAAG	2 cut(s)	1427, 1709
AgsI	TTSAA	5 cut(s)	550, 656, 1121, 1391, 2102
AjiI	CACGTC	1 cut(s)	108
AjnI	CCWGG	5 cut(s)	18, 177, 411, 810, 1195
AjuI	GAANNNNNNNTTGG	4 cut(s)	188, 220, 1749, 1781
AluBI	AGCT	9 cut(s)	15, 94, 199, 448, 535, 721, 785, 1784, 1971
AluI	AGCT	9 cut(s)	15, 94, 199, 448, 535, 721, 785, 1784, 1971
Alw26I	GTCTC	2 cut(s)	392, 680
AlwI	GGATC	6 cut(s)	704, 802, 872, 1645, 1850, 1919
AlwNI	CAGNNNCTG	3 cut(s)	437, 1043, 1562
Ama87I	CYCGRG	1 cut(s)	648
Aor51HI	AGCGCT	1 cut(s)	1534
AoxI	GGCC	5 cut(s)	70, 240, 279, 368, 474
ApeKI	GCWGC	4 cut(s)	419, 529, 532, 535
ApoI	RAATTY	4 cut(s)	1320, 1413, 1758, 2107
Asp700I	GAANNNNTTC	3 cut(s)	1368, 1726, 1758
AspLEI	GCGC	3 cut(s)	310, 1227, 1535
AspS9I	GGNCC	3 cut(s)	22, 175, 1592
AsuC2I	CCSGG	1 cut(s)	244
AsuHPI	GGTGA	1 cut(s)	37
AvaI	CYCGRG	1 cut(s)	648
AvaII	GGWCC	3 cut(s)	22, 175, 1592
BaeI	ACNNNNGTAYC	2 cut(s)	96, 129
BalI	TGGCCA	2 cut(s)	281, 476
BbsI	GAAGAC	2 cut(s)	1128, 1207
BbvI	GCAGC	4 cut(s)	406, 516, 522, 544
BccI	CCATC	9 cut(s)	505, 581, 614, 858, 959, 1238, 1559, 1784, 1952
BceAI	ACGGC	2 cut(s)	85, 255
BciT130I	CCWGG	5 cut(s)	20, 179, 413, 812, 1197
BcnI	CCSGG	1 cut(s)	244
BcoDI	GTCTC	2 cut(s)	392, 680
BfaI	CTAG	2 cut(s)	423, 2145
BfmI	CTRYAG	3 cut(s)	438, 530, 1170
BfoI	RGCGCY	2 cut(s)	311, 1536
BfrI	CTTAAG	2 cut(s)	1427, 1709
BisI	GCNGC	7 cut(s)	368, 420, 530, 533, 536, 595, 1703
BlsI	GCNGC	7 cut(s)	369, 421, 531, 534, 537, 596, 1704
BmcAI	AGTACT	1 cut(s)	1395
Bme1390I	CCNGG	6 cut(s)	20, 179, 244, 413, 812, 1197
Bme18I	GGWCC	3 cut(s)	22, 175, 1592
BmeT110I	CYCGRG	1 cut(s)	648
BmgBI	CACGTC	1 cut(s)	108
BmgT120I	GGNCC	3 cut(s)	22, 175, 1592
BmiI	GGNNCC	2 cut(s)	142, 228
BmrFI	CCNGG	6 cut(s)	20, 179, 244, 413, 812, 1197
BmsI	GCATC	6 cut(s)	656, 858, 880, 1168, 1860, 1900
BpiI	GAAGAC	2 cut(s)	1128, 1207
BplI	GAGNNNNNCTC	4 cut(s)	830, 862, 1247, 1279
BpmI	CTGGAG	1 cut(s)	1418
BpuEI	CTTGAG	4 cut(s)	470, 1086, 1172, 1646
BpuMI	CCSGG	1 cut(s)	244
BsaHI	GRCGYC	1 cut(s)	1644
BsaI	GGTCTC	1 cut(s)	392
BsaJI	CCNNGG	2 cut(s)	402, 477
BsaWI	WCCGGW	3 cut(s)	201, 229, 1730
BsaXI	ACNNNNNCTCC	2 cut(s)	20, 50
Bsc4I	CCNNNNNNNGG	6 cut(s)	483, 564, 587, 811, 887, 1639
Bse1I	ACTGG	4 cut(s)	1276, 1401, 1486, 1884
Bse3DI	GCAATG	1 cut(s)	1960
BseBI	CCWGG	5 cut(s)	20, 179, 413, 812, 1197
BseDI	CCNNGG	2 cut(s)	402, 477
BseGI	GGATG	4 cut(s)	172, 628, 873, 970
BseLI	CCNNNNNNNGG	6 cut(s)	483, 564, 587, 811, 887, 1639
BseMI	GCAATG	1 cut(s)	1960
BseMII	CTCAG	3 cut(s)	861, 1512, 1543
BseNI	ACTGG	4 cut(s)	1276, 1401, 1486, 1884
BseRI	GAGGAG	3 cut(s)	314, 317, 467
BseX3I	CGGCCG	1 cut(s)	240
BseXI	GCAGC	4 cut(s)	406, 516, 522, 544
BseYI	CCCAGC	1 cut(s)	679
BsgI	GTGCAG	3 cut(s)	1029, 1098, 1848
Bsh1236I	CGCG	3 cut(s)	156, 404, 1128
Bsh1285I	CGRYCG	2 cut(s)	154, 243
BshFI	GGCC	5 cut(s)	72, 242, 281, 370, 476
BsiEI	CGRYCG	2 cut(s)	154, 243
BsiHKCI	CYCGRG	1 cut(s)	648
BsiSI	CCGG	4 cut(s)	202, 230, 243, 1731
BslFI	GGGAC	3 cut(s)	419, 1226, 1807
BslI	CCNNNNNNNGG	6 cut(s)	483, 564, 587, 811, 887, 1639
BsmAI	GTCTC	2 cut(s)	392, 680
BsmFI	GGGAC	3 cut(s)	419, 1226, 1807
BsnI	GGCC	5 cut(s)	72, 242, 281, 370, 476
Bso31I	GGTCTC	1 cut(s)	392
BsoBI	CYCGRG	1 cut(s)	648
BspANI	GGCC	5 cut(s)	72, 242, 281, 370, 476
BspCNI	CTCAG	3 cut(s)	860, 1513, 1542
BspFNI	CGCG	3 cut(s)	156, 404, 1128
BspLI	GGNNCC	2 cut(s)	142, 228
BspMAI	CTGCAG	1 cut(s)	534
BspPI	GGATC	6 cut(s)	704, 802, 872, 1645, 1850, 1919
BspQI	GCTCTTC	1 cut(s)	101
BspTI	CTTAAG	2 cut(s)	1427, 1709
BspTNI	GGTCTC	1 cut(s)	392
BsrDI	GCAATG	1 cut(s)	1960
BsrI	ACTGG	4 cut(s)	1276, 1401, 1486, 1884
BssECI	CCNNGG	2 cut(s)	402, 477
BssNI	GRCGYC	1 cut(s)	1644
BssT1I	CCWWGG	1 cut(s)	477
Bst2UI	CCWGG	5 cut(s)	20, 179, 413, 812, 1197
Bst4CI	ACNGT	6 cut(s)	49, 433, 909, 1050, 1353, 2012
Bst6I	CTCTTC	2 cut(s)	101, 216
BstACI	GRCGYC	1 cut(s)	1644
BstAFI	CTTAAG	2 cut(s)	1427, 1709
BstAPI	GCANNNNNTGC	4 cut(s)	364, 675, 1043, 1507
BstC8I	GCNNGC	5 cut(s)	294, 1044, 1062, 1700, 1973
BstDEI	CTNAG	3 cut(s)	847, 1521, 1529
BstDSI	CCRYGG	1 cut(s)	402
BstENI	CCTNNNNNAGG	1 cut(s)	885
BstF5I	GGATG	4 cut(s)	172, 628, 873, 970
BstFNI	CGCG	3 cut(s)	156, 404, 1128
BstH2I	RGCGCY	2 cut(s)	311, 1536
BstHHI	GCGC	3 cut(s)	310, 1227, 1535
BstMAI	GTCTC	2 cut(s)	392, 680
BstMCI	CGRYCG	2 cut(s)	154, 243
BstMWI	GCNNNNNNNGC	7 cut(s)	364, 535, 675, 1043, 1507, 1781, 1910
BstNI	CCWGG	5 cut(s)	20, 179, 413, 812, 1197
BstNSI	RCATGY	2 cut(s)	1313, 1804
BstSCI	CCNGG	6 cut(s)	18, 177, 242, 411, 810, 1195
BstSFI	CTRYAG	3 cut(s)	438, 530, 1170
BstUI	CGCG	3 cut(s)	156, 404, 1128
BstV1I	GCAGC	4 cut(s)	406, 516, 522, 544
BstV2I	GAAGAC	2 cut(s)	1128, 1207
BstX2I	RGATCY	1 cut(s)	1924
BstXI	CCANNNNNNTGG	1 cut(s)	1935
BstYI	RGATCY	1 cut(s)	1924
BstZI	CGGCCG	1 cut(s)	240
BsuRI	GGCC	5 cut(s)	72, 242, 281, 370, 476
BtgI	CCRYGG	1 cut(s)	402
BtgZI	GCGATG	1 cut(s)	1143
BtrI	CACGTC	1 cut(s)	108
BtsCI	GGATG	4 cut(s)	172, 628, 873, 970
BtsI	GCAGTG	1 cut(s)	1616
BtsIMutI	CAGTG	6 cut(s)	616, 905, 1046, 1518, 1616, 2027
Cac8I	GCNNGC	5 cut(s)	294, 1044, 1062, 1700, 1973
CaiI	CAGNNNCTG	3 cut(s)	437, 1043, 1562
CfoI	GCGC	3 cut(s)	310, 1227, 1535
Cfr13I	GGNCC	3 cut(s)	22, 175, 1592
Cfr42I	CCGCGG	1 cut(s)	405
CseI	GACGC	1 cut(s)	145
CsiI	ACCWGGT	1 cut(s)	177
Csp6I	GTAC	4 cut(s)	113, 407, 947, 1394
CviAII	CATG	8 cut(s)	518, 619, 827, 1007, 1310, 1634, 1801, 1846
CviQI	GTAC	4 cut(s)	113, 407, 947, 1394
DdeI	CTNAG	3 cut(s)	847, 1521, 1529
DraI	TTTAAA	2 cut(s)	1444, 2035
DrdI	GACNNNNNNGTC	2 cut(s)	155, 1801
DseDI	GACNNNNNNGTC	2 cut(s)	155, 1801
EaeI	YGGCCR	3 cut(s)	240, 279, 474
EagI	CGGCCG	1 cut(s)	240
Eam1104I	CTCTTC	2 cut(s)	101, 216
EarI	CTCTTC	2 cut(s)	101, 216
EciI	GGCGGA	2 cut(s)	14, 311
EclXI	CGGCCG	1 cut(s)	240
Eco130I	CCWWGG	1 cut(s)	477
Eco31I	GGTCTC	1 cut(s)	392
Eco47I	GGWCC	3 cut(s)	22, 175, 1592
Eco47III	AGCGCT	1 cut(s)	1534
Eco52I	CGGCCG	1 cut(s)	240
Eco57I	CTGAAG	3 cut(s)	357, 544, 2067
Eco88I	CYCGRG	1 cut(s)	648
EcoNI	CCTNNNNNAGG	1 cut(s)	885
EcoRII	CCWGG	5 cut(s)	18, 177, 411, 810, 1195
EcoT14I	CCWWGG	1 cut(s)	477
EcoT22I	ATGCAT	1 cut(s)	873
ErhI	CCWWGG	1 cut(s)	477
FaeI	CATG	8 cut(s)	521, 622, 830, 1010, 1313, 1637, 1804, 1849
FaqI	GGGAC	3 cut(s)	419, 1226, 1807
FatI	CATG	8 cut(s)	517, 618, 826, 1006, 1309, 1633, 1800, 1845
FauI	CCCGC	1 cut(s)	914
FauNDI	CATATG	1 cut(s)	1888
FblI	GTMKAC	1 cut(s)	54
Fnu4HI	GCNGC	7 cut(s)	368, 420, 530, 533, 536, 595, 1703
FokI	GGATG	4 cut(s)	179, 635, 880, 977
Fsp4HI	GCNGC	7 cut(s)	368, 420, 530, 533, 536, 595, 1703
FspBI	CTAG	2 cut(s)	423, 2145
GlaI	GCGC	3 cut(s)	309, 1226, 1534
GluI	GCNGC	7 cut(s)	368, 420, 530, 533, 536, 595, 1703
GsaI	CCCAGC	1 cut(s)	683
GsuI	CTGGAG	1 cut(s)	1418
HaeII	RGCGCY	2 cut(s)	311, 1536
HaeIII	GGCC	5 cut(s)	72, 242, 281, 370, 476
HapII	CCGG	4 cut(s)	202, 230, 243, 1731
HgaI	GACGC	1 cut(s)	145
HhaI	GCGC	3 cut(s)	310, 1227, 1535
Hin1I	GRCGYC	1 cut(s)	1644
Hin1II	CATG	8 cut(s)	521, 622, 830, 1010, 1313, 1637, 1804, 1849
Hin6I	GCGC	3 cut(s)	308, 1225, 1533
HinP1I	GCGC	3 cut(s)	308, 1225, 1533
HincII	GTYRAC	1 cut(s)	1576
HindII	GTYRAC	1 cut(s)	1576
HindIII	AAGCTT	1 cut(s)	719
HinfI	GANTC	9 cut(s)	35, 170, 233, 688, 797, 851, 1402, 1466, 1688
HpaII	CCGG	4 cut(s)	202, 230, 243, 1731
HphI	GGTGA	1 cut(s)	37
Hpy166II	GTNNAC	4 cut(s)	55, 1286, 1303, 1576
Hpy8I	GTNNAC	4 cut(s)	55, 1286, 1303, 1576
Hpy99I	CGWCG	6 cut(s)	53, 68, 71, 161, 241, 1026
HpyAV	CCTTC	9 cut(s)	451, 568, 729, 881, 955, 1129, 1358, 1826, 2042
HpyCH4III	ACNGT	6 cut(s)	49, 433, 909, 1050, 1353, 2012
HpyCH4IV	ACGT	3 cut(s)	107, 1024, 1644
HpyF10VI	GCNNNNNNNGC	7 cut(s)	364, 535, 675, 1043, 1507, 1781, 1910
HpyF3I	CTNAG	3 cut(s)	847, 1521, 1529
HpySE526I	ACGT	3 cut(s)	107, 1024, 1644
Hsp92I	GRCGYC	1 cut(s)	1644
Hsp92II	CATG	8 cut(s)	521, 622, 830, 1010, 1313, 1637, 1804, 1849
HspAI	GCGC	3 cut(s)	308, 1225, 1533
KspI	CCGCGG	1 cut(s)	405
LguI	GCTCTTC	1 cut(s)	101
LmnI	GCTCC	5 cut(s)	12, 140, 204, 347, 1268
Lsp1109I	GCAGC	4 cut(s)	406, 516, 522, 544
LweI	GCATC	6 cut(s)	656, 858, 880, 1168, 1860, 1900
MabI	ACCWGGT	1 cut(s)	177
MaeI	CTAG	2 cut(s)	423, 2145
MaeII	ACGT	3 cut(s)	107, 1024, 1644
MaeIII	GTNAC	6 cut(s)	979, 1025, 1312, 1524, 1624, 1645
MflI	RGATCY	1 cut(s)	1924
MlsI	TGGCCA	2 cut(s)	281, 476
MluNI	TGGCCA	2 cut(s)	281, 476
MlyI	GAGTC	2 cut(s)	29, 682
MmeI	TCCRAC	2 cut(s)	1064, 1820
Mox20I	TGGCCA	2 cut(s)	281, 476
Mph1103I	ATGCAT	1 cut(s)	873
MroXI	GAANNNNTTC	3 cut(s)	1368, 1726, 1758
MscI	TGGCCA	2 cut(s)	281, 476
MslI	CAYNNNNRTG	1 cut(s)	1731
Msp20I	TGGCCA	2 cut(s)	281, 476
MspA1I	CMGCKG	2 cut(s)	404, 535
MspCI	CTTAAG	2 cut(s)	1427, 1709
MspI	CCGG	4 cut(s)	202, 230, 243, 1731
MspR9I	CCNGG	6 cut(s)	20, 179, 244, 413, 812, 1197
MvaI	CCWGG	5 cut(s)	20, 179, 413, 812, 1197
MvnI	CGCG	3 cut(s)	156, 404, 1128
MwoI	GCNNNNNNNGC	7 cut(s)	364, 535, 675, 1043, 1507, 1781, 1910
NciI	CCSGG	1 cut(s)	244
NdeI	CATATG	1 cut(s)	1888
NlaIII	CATG	8 cut(s)	521, 622, 830, 1010, 1313, 1637, 1804, 1849
NlaIV	GGNNCC	2 cut(s)	142, 228
NmeAIII	GCCGAG	1 cut(s)	806
NmuCI	GTSAC	2 cut(s)	1312, 1645
NsiI	ATGCAT	1 cut(s)	873
NspI	RCATGY	2 cut(s)	1313, 1804
PaeR7I	CTCGAG	1 cut(s)	648
PciSI	GCTCTTC	1 cut(s)	101
PcsI	WCGNNNNNNNCGW	3 cut(s)	63, 72, 156
PdmI	GAANNNNTTC	3 cut(s)	1368, 1726, 1758
PfeI	GAWTC	7 cut(s)	170, 233, 797, 851, 1402, 1466, 1688
PflFI	GACNNNGTC	1 cut(s)	155
PflMI	CCANNNNNTGG	1 cut(s)	1639
PfoI	TCCNGGA	1 cut(s)	810
PkrI	GCNGC	7 cut(s)	369, 421, 531, 534, 537, 596, 1704
PleI	GAGTC	2 cut(s)	29, 682
PpsI	GAGTC	2 cut(s)	29, 682
Psp6I	CCWGG	5 cut(s)	18, 177, 411, 810, 1195
PspFI	CCCAGC	1 cut(s)	679
PspGI	CCWGG	5 cut(s)	18, 177, 411, 810, 1195
PspN4I	GGNNCC	2 cut(s)	142, 228
PspPI	GGNCC	3 cut(s)	22, 175, 1592
PstI	CTGCAG	1 cut(s)	534
PstNI	CAGNNNCTG	3 cut(s)	437, 1043, 1562
PsuI	RGATCY	1 cut(s)	1924
PsyI	GACNNNGTC	1 cut(s)	155
PvuII	CAGCTG	1 cut(s)	535
RsaI	GTAC	4 cut(s)	114, 408, 948, 1395
RsaNI	GTAC	4 cut(s)	113, 407, 947, 1394
RseI	CAYNNNNRTG	1 cut(s)	1731
SacII	CCGCGG	1 cut(s)	405
SapI	GCTCTTC	1 cut(s)	101
SatI	GCNGC	7 cut(s)	368, 420, 530, 533, 536, 595, 1703
Sau96I	GGNCC	3 cut(s)	22, 175, 1592
ScaI	AGTACT	1 cut(s)	1395
SchI	GAGTC	2 cut(s)	29, 682
ScrFI	CCNGG	6 cut(s)	20, 179, 244, 413, 812, 1197
SexAI	ACCWGGT	1 cut(s)	177
SfaNI	GCATC	6 cut(s)	656, 858, 880, 1168, 1860, 1900
SfcI	CTRYAG	3 cut(s)	438, 530, 1170
Sfr274I	CTCGAG	1 cut(s)	648
Sfr303I	CCGCGG	1 cut(s)	405
SgrBI	CCGCGG	1 cut(s)	405
SinI	GGWCC	3 cut(s)	22, 175, 1592
SlaI	CTCGAG	1 cut(s)	648
SmiMI	CAYNNNNRTG	1 cut(s)	1731
SmlI	CTYRAG	7 cut(s)	449, 648, 1101, 1151, 1427, 1661, 1709
SmoI	CTYRAG	7 cut(s)	449, 648, 1101, 1151, 1427, 1661, 1709
SspMI	CTAG	2 cut(s)	423, 2145
StyD4I	CCNGG	6 cut(s)	18, 177, 242, 411, 810, 1195
StyI	CCWWGG	1 cut(s)	477
TaaI	ACNGT	6 cut(s)	49, 433, 909, 1050, 1353, 2012
TaiI	ACGT	3 cut(s)	110, 1027, 1647
TaqI	TCGA	8 cut(s)	75, 212, 236, 649, 1021, 1944, 2007, 2113
TatI	WGTACW	2 cut(s)	946, 1393
TauI	GCSGC	3 cut(s)	370, 597, 1705
TfiI	GAWTC	7 cut(s)	170, 233, 797, 851, 1402, 1466, 1688
TscAI	CASTG	6 cut(s)	616, 912, 1053, 1525, 1623, 2027
TseFI	GTSAC	2 cut(s)	1312, 1645
TseI	GCWGC	4 cut(s)	419, 529, 532, 535
Tsp45I	GTSAC	2 cut(s)	1312, 1645
TspDTI	ATGAA	8 cut(s)	522, 588, 626, 810, 930, 1377, 1530, 2031
TspGWI	ACGGA	2 cut(s)	1643, 1730
TspRI	CASTG	6 cut(s)	616, 912, 1053, 1525, 1623, 2027
Tth111I	GACNNNGTC	1 cut(s)	155
Van91I	CCANNNNNTGG	1 cut(s)	1639
Vha464I	CTTAAG	2 cut(s)	1427, 1709
VpaK11BI	GGWCC	3 cut(s)	22, 175, 1592
XagI	CCTNNNNNAGG	1 cut(s)	885
XapI	RAATTY	4 cut(s)	1320, 1413, 1758, 2107
XbaI	TCTAGA	1 cut(s)	2144
XceI	RCATGY	2 cut(s)	1313, 1804
XhoI	CTCGAG	1 cut(s)	648
XmiI	GTMKAC	1 cut(s)	54
XmnI	GAANNNNTTC	3 cut(s)	1368, 1726, 1758
XspI	CTAG	2 cut(s)	423, 2145
ZraI	GACGTC	1 cut(s)	1645
ZrmI	AGTACT	1 cut(s)	1395
Zsp2I	ATGCAT	1 cut(s)	873

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.