Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009813

Physical Location & Seq

Genomic Coordinates Forward (+)

28360918 .. 28364464

Gene Structure 3547 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1194200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGGCGTTTAGGGTTCGAAACAGCTCGAGCTTGCTGTCCCTGGTCCTTCTCTCTCTCCTCGCCATCGCCTCCGCCAAGGTTTTCTTCGAAGAGCGCTTCGAAGATGGATGGGACAAACGTTGGGTGAAATCTGACTGGAAGAGAGACGAGGGCCTTGCCGGAGAATGGAACTACACCTCTGGAAAATGGAATGGAGATGCTAATGACAAAGGTATCCAAACAAGTGAGGACTATAGGTTTTATGCCATTTCAGCTGAGTTCCCTGAATTCAGCAACAAGGACAAGACACTAGTGTTCCAATTTTCCGTGAAACATGAGCAGAAGCTTGACTGTGGTGGTGGCTATATCAAGTTACTCAGTGGTGACGTTGATCAGAAGAAATTCGGCGGTGATACCCCATACAGTATCATGTTTGGGCCAGATATCTGTGGATACACCACCAAGAAAGTTCACGCAATCCTTAACTACAATGACACAAATAAATTGATCAAGAAGGACGTCCCCTGTGAGACTGACCAACTCACTCATGTTTATACTTTCATCCTCCGCCCAGATGCTACCTACAGCATCCTTATCGACAATGTAGAGAAACAAACTGGTAGCCTATACAGTGATTGGGATCTTCTGCCACCAAAGAAAATCAAGGATCCTGAGGCCAAGAAACCTGAAGATTGGGAAGACAAAGAATACATTCCTGACCCTGAAGATACTAAGCCAGAGGGTTATGATGACATTCCCAAGGAGATAACCGATCCCGATGCCAAGAAGCCGGAAGATTGGGATGATGAGGAAGATGGTGAATGGACTGCCCCAACCATTCCCAACCCTGAATACAAGGGTGAATGGAAGCCAAAGAAAATCAAGAACCCCAACTTCAAGGGAAAGTGGAAGGCACCACTGATTGACAACCCAGAGTTCAAGGATGACCCTGAGCTGTATGTTTACCCCAACTTGAAGTATGTTGGAATTGAATTGTGGCAGGTGAAATCTGGAACCCTGTTTGACAATATCTTGATCACTGATGAGCCGGAGTATGCAAAGCAACTGGCTGAGGAAACATGGGGCAAACAAAAAGATGCCGAGAAGGCAGCATTTGAAGAGGCAGAGAAAAAGCAGGAGGAGGAAGCAAAAGATCCAGTTGACTCTGATGCTGAGGAAGAGGATGATGCTGACACAGATGATGCTGAAGATGACTCTGATGCGGAATCTAAATCGGACTCAACTGAAGAGAGCGCCGAGGAGTCTGAGAAACATGATGAACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005783 GO:0005788 GO:0005911 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0010035 GO:0010038 GO:0012505 GO:0016020 GO:0030054 GO:0031090 GO:0031974 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0055044 GO:0070013 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04141 ko04145 ko04612 ko05142 ko05166 map04141 map04145 map04612 map05142 map05166

Pfam Domains

Protein Families

Calreticulin

Protein Analysis

422

Amino Acids

48.33

Weight (kDa)

4.42

Isoelectric Point (pI)

36.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calreticulin	PF00262	29 - 264	3.6e-59	Calreticulin family
Calreticulin	PF00262	266 - 339	8.9e-21	Calreticulin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009813)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09210 AT1G56340 AT1G56340
fragaria_vesca	FvH4_6g07360
malus_domestica	MD04G1194200.v1.1 MD12G1206700.v1.1
prunus_persica	Prupe.6G313900_v2.0.a1
pyrus_communis	pycom04g17170 pycom12g19460
rosa_chinensis	RchiOBHm_Chr3g0456961
rosa_laevigata	RLG00000025259
rosa_multiflora	Rmu_sc0016008.1_g000004 Rmu_sc0032731.1_g000001
rosa_roxburghii	Rroxscaffold_6G00422330
rosa_rugosa	Rorug03G0017700 Rorug03G0017800
rosa_samantha	Rh3AG077700 Rh3BG079600 Rh3CG079500 Rh3DG081300
rosa_wichuraiana	Rw3G006660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	970
AatII	GACGTC	1 cut(s)	501
Acc36I	ACCTGC	1 cut(s)	970
AccB1I	GGYRCC	1 cut(s)	892
AciI	CCGC	4 cut(s)	72, 387, 547, 1202
AclI	AACGTT	1 cut(s)	118
AclWI	GGATC	5 cut(s)	627, 641, 654, 746, 1127
AcsI	RAATTY	2 cut(s)	266, 380
AcuI	CTGAAG	4 cut(s)	687, 723, 1206, 1245
AcyI	GRCGYC	1 cut(s)	498
AfeI	AGCGCT	1 cut(s)	95
AfiI	CCNNNNNNNGG	1 cut(s)	165
AgsI	TTSAA	5 cut(s)	877, 919, 955, 971, 1097
AhlI	ACTAGT	1 cut(s)	289
AjnI	CCWGG	1 cut(s)	39
AjuI	GAANNNNNNNTTGG	2 cut(s)	68, 100
AluBI	AGCT	5 cut(s)	24, 30, 254, 325, 934
AluI	AGCT	5 cut(s)	24, 30, 254, 325, 934
Alw26I	GTCTC	2 cut(s)	138, 503
AlwI	GGATC	5 cut(s)	627, 641, 654, 746, 1127
Ama87I	CYCGRG	1 cut(s)	25
Aor51HI	AGCGCT	1 cut(s)	95
AoxI	GGCC	3 cut(s)	151, 416, 654
ApeKI	GCWGC	1 cut(s)	1088
ApoI	RAATTY	2 cut(s)	266, 380
Asp700I	GAANNNNTTC	2 cut(s)	380, 690
AspLEI	GCGC	2 cut(s)	96, 1235
AspS9I	GGNCC	3 cut(s)	43, 151, 416
AsuHPI	GGTGA	6 cut(s)	136, 374, 401, 809, 851, 994
AsuII	TTCGAA	3 cut(s)	16, 87, 99
AvaI	CYCGRG	1 cut(s)	25
AvaII	GGWCC	1 cut(s)	43
AxyI	CCTNAGG	1 cut(s)	651
BaeI	ACNNNNGTAYC	2 cut(s)	196, 229
BamHI	GGATCC	1 cut(s)	646
BanI	GGYRCC	1 cut(s)	892
BbsI	GAAGAC	1 cut(s)	684
BbvCI	CCTCAGC	2 cut(s)	1050, 1152
BbvI	GCAGC	1 cut(s)	1100
BccI	CCATC	4 cut(s)	71, 98, 102, 788
BcgI	CGANNNNNNTGC	6 cut(s)	137, 171, 556, 590, 740, 774
BciT130I	CCWGG	1 cut(s)	41
BciVI	GTATCC	2 cut(s)	224, 425
BclI	TGATCA	3 cut(s)	370, 486, 1014
BcoDI	GTCTC	2 cut(s)	138, 503
BcuI	ACTAGT	1 cut(s)	289
BfaI	CTAG	1 cut(s)	290
BfmI	CTRYAG	3 cut(s)	232, 562, 1262
BfoI	RGCGCY	2 cut(s)	97, 1236
BfuAI	ACCTGC	1 cut(s)	970
BfuI	GTATCC	2 cut(s)	224, 425
BglI	GCCNNNNNGGC	1 cut(s)	1085
BisI	GCNGC	1 cut(s)	1089
BlsI	GCNGC	1 cut(s)	1090
Bme1390I	CCNGG	1 cut(s)	41
Bme18I	GGWCC	1 cut(s)	43
BmeT110I	CYCGRG	1 cut(s)	25
BmgT120I	GGNCC	3 cut(s)	43, 151, 416
BmiI	GGNNCC	3 cut(s)	648, 894, 994
BmrFI	CCNGG	1 cut(s)	41
BmsI	GCATC	9 cut(s)	187, 544, 576, 748, 1066, 1138, 1156, 1171, 1189
BpiI	GAAGAC	1 cut(s)	684
Bpu10I	CCTNAGC	3 cut(s)	930, 1050, 1152
Bpu14I	TTCGAA	3 cut(s)	16, 87, 99
BsaBI	GATNNNNATC	1 cut(s)	618
BsaHI	GRCGYC	1 cut(s)	498
BsaJI	CCNNGG	4 cut(s)	39, 75, 738, 1236
Bsc4I	CCNNNNNNNGG	1 cut(s)	165
Bse1I	ACTGG	4 cut(s)	140, 601, 1050, 1136
Bse21I	CCTNAGG	1 cut(s)	651
Bse8I	GATNNNNATC	1 cut(s)	618
BseBI	CCWGG	1 cut(s)	41
BseDI	CCNNGG	4 cut(s)	39, 75, 738, 1236
BseGI	GGATG	6 cut(s)	113, 540, 567, 787, 928, 1168
BseJI	GATNNNNATC	1 cut(s)	618
BseLI	CCNNNNNNNGG	1 cut(s)	165
BseMII	CTCAG	7 cut(s)	246, 370, 642, 921, 1041, 1143, 1236
BseNI	ACTGG	4 cut(s)	140, 601, 1050, 1136
BseRI	GAGGAG	3 cut(s)	47, 1133, 1253
BseXI	GCAGC	1 cut(s)	1100
BshFI	GGCC	3 cut(s)	153, 418, 656
BshNI	GGYRCC	1 cut(s)	892
BsiHKCI	CYCGRG	1 cut(s)	25
BsiSI	CCGG	3 cut(s)	159, 770, 1028
BslFI	GGGAC	3 cut(s)	22, 125, 485
BslI	CCNNNNNNNGG	1 cut(s)	165
BsmAI	GTCTC	2 cut(s)	138, 503
BsmBI	CGTCTC	1 cut(s)	138
BsmFI	GGGAC	3 cut(s)	22, 125, 485
BsnI	GGCC	3 cut(s)	153, 418, 656
BsoBI	CYCGRG	1 cut(s)	25
Bsp119I	TTCGAA	3 cut(s)	16, 87, 99
Bsp143I	GATC	7 cut(s)	370, 486, 619, 646, 751, 1014, 1132
BspACI	CCGC	4 cut(s)	72, 387, 547, 1202
BspANI	GGCC	3 cut(s)	153, 418, 656
BspCNI	CTCAG	7 cut(s)	247, 369, 643, 922, 1042, 1144, 1237
BspLI	GGNNCC	3 cut(s)	648, 894, 994
BspMI	ACCTGC	1 cut(s)	970
BspPI	GGATC	5 cut(s)	627, 641, 654, 746, 1127
BspQI	GCTCTTC	1 cut(s)	84
BspT104I	TTCGAA	3 cut(s)	16, 87, 99
BspT107I	GGYRCC	1 cut(s)	892
BsrI	ACTGG	4 cut(s)	140, 601, 1050, 1136
BssECI	CCNNGG	4 cut(s)	39, 75, 738, 1236
BssMI	GATC	7 cut(s)	370, 486, 619, 646, 751, 1014, 1132
BssNI	GRCGYC	1 cut(s)	498
BssT1I	CCWWGG	2 cut(s)	75, 738
Bst2UI	CCWGG	1 cut(s)	41
Bst4CI	ACNGT	4 cut(s)	332, 404, 611, 1263
Bst6I	CTCTTC	5 cut(s)	84, 134, 1092, 1152, 1221
BstACI	GRCGYC	1 cut(s)	498
BstBI	TTCGAA	3 cut(s)	16, 87, 99
BstC8I	GCNNGC	1 cut(s)	32
BstDEI	CTNAG	8 cut(s)	255, 356, 651, 711, 930, 1050, 1152, 1245
BstF5I	GGATG	6 cut(s)	113, 540, 567, 787, 928, 1168
BstH2I	RGCGCY	2 cut(s)	97, 1236
BstHHI	GCGC	2 cut(s)	96, 1235
BstKTI	GATC	7 cut(s)	373, 489, 622, 649, 754, 1017, 1135
BstMAI	GTCTC	2 cut(s)	138, 503
BstMBI	GATC	7 cut(s)	370, 486, 619, 646, 751, 1014, 1132
BstMWI	GCNNNNNNNGC	2 cut(s)	251, 1085
BstNI	CCWGG	1 cut(s)	41
BstSCI	CCNGG	1 cut(s)	39
BstSFI	CTRYAG	3 cut(s)	232, 562, 1262
BstV1I	GCAGC	1 cut(s)	1100
BstV2I	GAAGAC	1 cut(s)	684
BstX2I	RGATCY	3 cut(s)	619, 646, 1132
BstYI	RGATCY	3 cut(s)	619, 646, 1132
Bsu36I	CCTNAGG	1 cut(s)	651
BsuI	GTATCC	2 cut(s)	224, 425
BsuRI	GGCC	3 cut(s)	153, 418, 656
BtgZI	GCGATG	1 cut(s)	49
BtsCI	GGATG	6 cut(s)	113, 540, 567, 787, 928, 1168
BtsIMutI	CAGTG	4 cut(s)	364, 616, 896, 1017
BveI	ACCTGC	1 cut(s)	970
Cac8I	GCNNGC	1 cut(s)	32
CfoI	GCGC	2 cut(s)	96, 1235
Cfr13I	GGNCC	3 cut(s)	43, 151, 416
CviAII	CATG	5 cut(s)	314, 409, 527, 1059, 1253
DdeI	CTNAG	8 cut(s)	255, 356, 651, 711, 930, 1050, 1152, 1245
DpnI	GATC	7 cut(s)	372, 488, 621, 648, 753, 1016, 1134
DpnII	GATC	7 cut(s)	370, 486, 619, 646, 751, 1014, 1132
Eam1104I	CTCTTC	5 cut(s)	84, 134, 1092, 1152, 1221
EarI	CTCTTC	5 cut(s)	84, 134, 1092, 1152, 1221
EciI	GGCGGA	2 cut(s)	61, 536
Eco130I	CCWWGG	2 cut(s)	75, 738
Eco32I	GATATC	1 cut(s)	424
Eco47I	GGWCC	1 cut(s)	43
Eco47III	AGCGCT	1 cut(s)	95
Eco57I	CTGAAG	4 cut(s)	687, 723, 1206, 1245
Eco81I	CCTNAGG	1 cut(s)	651
Eco88I	CYCGRG	1 cut(s)	25
EcoO109I	RGGNCCY	1 cut(s)	151
EcoRI	GAATTC	1 cut(s)	266
EcoRII	CCWGG	1 cut(s)	39
EcoRV	GATATC	1 cut(s)	424
EcoT14I	CCWWGG	2 cut(s)	75, 738
ErhI	CCWWGG	2 cut(s)	75, 738
Esp3I	CGTCTC	1 cut(s)	138
FaeI	CATG	5 cut(s)	317, 412, 530, 1062, 1256
FalI	AAGNNNNNCTT	2 cut(s)	68, 100
FaqI	GGGAC	3 cut(s)	22, 125, 485
FatI	CATG	5 cut(s)	313, 408, 526, 1058, 1252
FbaI	TGATCA	3 cut(s)	370, 486, 1014
Fnu4HI	GCNGC	1 cut(s)	1089
FokI	GGATG	6 cut(s)	120, 527, 554, 794, 935, 1175
Fsp4HI	GCNGC	1 cut(s)	1089
FspBI	CTAG	1 cut(s)	290
GlaI	GCGC	2 cut(s)	95, 1234
GluI	GCNGC	1 cut(s)	1089
HaeII	RGCGCY	2 cut(s)	97, 1236
HaeIII	GGCC	3 cut(s)	153, 418, 656
HapII	CCGG	3 cut(s)	159, 770, 1028
HhaI	GCGC	2 cut(s)	96, 1235
Hin1I	GRCGYC	1 cut(s)	498
Hin1II	CATG	5 cut(s)	317, 412, 530, 1062, 1256
Hin6I	GCGC	2 cut(s)	94, 1233
HinP1I	GCGC	2 cut(s)	94, 1233
HincII	GTYRAC	1 cut(s)	1141
HindII	GTYRAC	1 cut(s)	1141
HindIII	AAGCTT	1 cut(s)	323
HinfI	GANTC	5 cut(s)	1142, 1193, 1205, 1217, 1241
HpaII	CCGG	3 cut(s)	159, 770, 1028
HphI	GGTGA	6 cut(s)	136, 374, 401, 809, 851, 994
Hpy166II	GTNNAC	3 cut(s)	451, 943, 1141
Hpy188I	TCNGA	6 cut(s)	133, 375, 1147, 1198, 1216, 1246
Hpy188III	TCNNGA	8 cut(s)	180, 490, 650, 695, 755, 862, 990, 1012
Hpy8I	GTNNAC	3 cut(s)	451, 943, 1141
HpyAV	CCTTC	4 cut(s)	56, 487, 883, 1078
HpyCH4III	ACNGT	4 cut(s)	332, 404, 611, 1263
HpyCH4IV	ACGT	3 cut(s)	118, 366, 498
HpyCH4V	TGCA	1 cut(s)	1037
HpyF10VI	GCNNNNNNNGC	2 cut(s)	251, 1085
HpyF3I	CTNAG	8 cut(s)	255, 356, 651, 711, 930, 1050, 1152, 1245
HpySE526I	ACGT	3 cut(s)	118, 366, 498
Hsp92I	GRCGYC	1 cut(s)	498
Hsp92II	CATG	5 cut(s)	317, 412, 530, 1062, 1256
HspAI	GCGC	2 cut(s)	94, 1233
Ksp22I	TGATCA	3 cut(s)	370, 486, 1014
Kzo9I	GATC	7 cut(s)	370, 486, 619, 646, 751, 1014, 1132
LguI	GCTCTTC	1 cut(s)	84
Lsp1109I	GCAGC	1 cut(s)	1100
LweI	GCATC	9 cut(s)	187, 544, 576, 748, 1066, 1138, 1156, 1171, 1189
MaeI	CTAG	1 cut(s)	290
MaeII	ACGT	3 cut(s)	118, 366, 498
MaeIII	GTNAC	2 cut(s)	351, 362
MalI	GATC	7 cut(s)	372, 488, 621, 648, 753, 1016, 1134
MboI	GATC	7 cut(s)	370, 486, 619, 646, 751, 1014, 1132
MflI	RGATCY	3 cut(s)	619, 646, 1132
MluCI	AATT	6 cut(s)	266, 299, 380, 482, 966, 971
MlyI	GAGTC	4 cut(s)	1136, 1187, 1211, 1250
MmeI	TCCRAC	1 cut(s)	943
MroXI	GAANNNNTTC	2 cut(s)	380, 690
MseI	TTAA	1 cut(s)	462
MspA1I	CMGCKG	1 cut(s)	254
MspI	CCGG	3 cut(s)	159, 770, 1028
MspR9I	CCNGG	1 cut(s)	41
MvaI	CCWGG	1 cut(s)	41
MwoI	GCNNNNNNNGC	2 cut(s)	251, 1085
NdeII	GATC	7 cut(s)	370, 486, 619, 646, 751, 1014, 1132
NlaIII	CATG	5 cut(s)	317, 412, 530, 1062, 1256
NlaIV	GGNNCC	3 cut(s)	648, 894, 994
NmeAIII	GCCGAG	2 cut(s)	1105, 1261
NmuCI	GTSAC	1 cut(s)	362
NspV	TTCGAA	3 cut(s)	16, 87, 99
PaeR7I	CTCGAG	1 cut(s)	25
PaqCI	CACCTGC	1 cut(s)	970
PciSI	GCTCTTC	1 cut(s)	84
PdmI	GAANNNNTTC	2 cut(s)	380, 690
PfeI	GAWTC	1 cut(s)	1205
PkrI	GCNGC	1 cut(s)	1090
PleI	GAGTC	4 cut(s)	1136, 1187, 1211, 1249
PpsI	GAGTC	4 cut(s)	1136, 1187, 1211, 1249
Psp1406I	AACGTT	1 cut(s)	118
Psp6I	CCWGG	1 cut(s)	39
PspGI	CCWGG	1 cut(s)	39
PspN4I	GGNNCC	3 cut(s)	648, 894, 994
PspPI	GGNCC	3 cut(s)	43, 151, 416
PspXI	VCTCGAGB	1 cut(s)	25
PsuI	RGATCY	3 cut(s)	619, 646, 1132
PvuII	CAGCTG	1 cut(s)	254
SapI	GCTCTTC	1 cut(s)	84
SaqAI	TTAA	1 cut(s)	462
SatI	GCNGC	1 cut(s)	1089
Sau3AI	GATC	7 cut(s)	370, 486, 619, 646, 751, 1014, 1132
Sau96I	GGNCC	3 cut(s)	43, 151, 416
SchI	GAGTC	4 cut(s)	1136, 1187, 1211, 1250
ScrFI	CCNGG	1 cut(s)	41
SfaNI	GCATC	9 cut(s)	187, 544, 576, 748, 1066, 1138, 1156, 1171, 1189
SfcI	CTRYAG	3 cut(s)	232, 562, 1262
Sfr274I	CTCGAG	1 cut(s)	25
SfuI	TTCGAA	3 cut(s)	16, 87, 99
SinI	GGWCC	1 cut(s)	43
SlaI	CTCGAG	1 cut(s)	25
SmlI	CTYRAG	1 cut(s)	25
SmoI	CTYRAG	1 cut(s)	25
SpeI	ACTAGT	1 cut(s)	289
Sse9I	AATT	6 cut(s)	266, 299, 380, 482, 966, 971
SsiI	CCGC	4 cut(s)	72, 387, 547, 1202
SspMI	CTAG	1 cut(s)	290
StyD4I	CCNGG	1 cut(s)	39
StyI	CCWWGG	2 cut(s)	75, 738
TaaI	ACNGT	4 cut(s)	332, 404, 611, 1263
TaiI	ACGT	3 cut(s)	121, 369, 501
TaqI	TCGA	5 cut(s)	16, 26, 87, 99, 576
TasI	AATT	6 cut(s)	266, 299, 380, 482, 966, 971
TfiI	GAWTC	1 cut(s)	1205
Tru1I	TTAA	1 cut(s)	462
Tru9I	TTAA	1 cut(s)	462
TscAI	CASTG	4 cut(s)	364, 616, 903, 1024
TseFI	GTSAC	1 cut(s)	362
TseI	GCWGC	1 cut(s)	1088
Tsp45I	GTSAC	1 cut(s)	362
TspDTI	ATGAA	1 cut(s)	529
TspGWI	ACGGA	1 cut(s)	295
TspRI	CASTG	4 cut(s)	364, 616, 903, 1024
VpaK11BI	GGWCC	1 cut(s)	43
XapI	RAATTY	2 cut(s)	266, 380
XhoI	CTCGAG	1 cut(s)	25
XmnI	GAANNNNTTC	2 cut(s)	380, 690
XspI	CTAG	1 cut(s)	290
ZraI	GACGTC	1 cut(s)	499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.