Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009813

Physical Location & Seq

Genomic Coordinates Forward (+)

28706758 .. 28710423

Gene Structure 3666 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1206700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGGCGTTTAGGGTTCGAACCAACTCGAGCTTGCTGCCCCTGGTCCTTCTCTCTCTCCTCGCCATCGCCTCCGCCAAGGTTTTCTTCGAAGAACGGTTCGAAGACGGATGGGATAAACGTTGGGTTAAATCTGACTGGAAGAGAGATGAGAGCCTTGCTGGAGAATGGAACTACACCTCTGGCAAATGGAATGGAGATGCTAATGACAAGGGCATCCAAACAAGTGAGGATTATAGGTTTTATGCCATTTCAGCTGAGTTCCCTGAATTCAGCAACAAGGACAAGACACTAGTGTTCCAATTTTCCGTGAAACATGAGCAGAAGCTTGACTGTGGTGGTGGCTACATCAAGTTACTCAGCGGTGATGTTGATCAGAAGAAATTTGGCGGTGATACCCCATACAGTATCATGTTTGGGCCAGATATCTGTGGATACAGCACCAAGAAAGTTCACGCAATTCTTAACTACAATAACACAAATAACTTGATCAAGAAGGACGTCCCTTGTGAGACTGATCAGCTTACTCATGTTTATACCTTTATCCTCCGCCCAGATGCTACCTACAGCATCCTTATTGACAATGTAGAGAAACAAACTGGCAGCCTCTACAGTGATTGGGATCTTCTGCCTCCAAAGAAAATCAAGGATCCCGAGGCCAAGAAACCTGAAGATTGGGATGACAAAGAATACATTCCTGACCCCGAAGATACTAAGCCAGAGGGTTATGATGACATTCCCAAGGAGATAGTAGATCCCGAAGCCAAGAAGCCGGAAGATTGGGATGATGAGGAAGATGGTGAATGGACTGCCCCAACCATTCCCAACCCCGAATACAAGGGTGAATGGAAGCCAAAGAAAATCAAGAACCCCAACTTCAAGGGAAAGTGGAAGGCACCACTCATTGACAACCCAAAGTTCAAGGATGACCCTGAGCTGTATGTTTACCCCAACTTGAAGTATGTCGGAATCGAATTGTGGCAGGTGAAATCTGGAACCCTGTTTGACAATATCTTGATCGCTGAAGACCCGGAGTATGCAAAGCAGCTGGCTGACGAAACGTGGGGAAAACAAAAAGATGCTGAGAAGGCAGCATTCGAGGAAGCAGAGAAACTGCAGGAGGAGGAAGCAAAAGACCCAGTTGATTCTGATGCCGAGGAAGAAGATGATGCTGACGCCGATGATGCGGAAGATGACTCTGACGCCGAATCTAAACCAGACTCAACTGAAGAGAGCGCCGAGGAGGCTGAGAAACATGACGAACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005783 GO:0005788 GO:0005911 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0010035 GO:0010038 GO:0012505 GO:0016020 GO:0030054 GO:0031090 GO:0031974 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0055044 GO:0070013 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04141 ko04145 ko04612 ko05142 ko05166 map04141 map04145 map04612 map05142 map05166

Pfam Domains

Protein Families

Calreticulin

Protein Analysis

422

Amino Acids

48.25

Weight (kDa)

4.42

Isoelectric Point (pI)

33.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calreticulin	PF00262	29 - 264	3.4e-60	Calreticulin family
Calreticulin	PF00262	266 - 339	1.1e-20	Calreticulin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009813)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09210 AT1G56340 AT1G56340
fragaria_vesca	FvH4_6g07360
malus_domestica	MD04G1194200.v1.1 MD12G1206700.v1.1
prunus_persica	Prupe.6G313900_v2.0.a1
pyrus_communis	pycom04g17170 pycom12g19460
rosa_chinensis	RchiOBHm_Chr3g0456961
rosa_laevigata	RLG00000025259
rosa_multiflora	Rmu_sc0016008.1_g000004 Rmu_sc0032731.1_g000001
rosa_roxburghii	Rroxscaffold_6G00422330
rosa_rugosa	Rorug03G0017700 Rorug03G0017800
rosa_samantha	Rh3AG077700 Rh3BG079600 Rh3CG079500 Rh3DG081300
rosa_wichuraiana	Rw3G006660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	970
AatII	GACGTC	1 cut(s)	501
Acc36I	ACCTGC	1 cut(s)	970
AccB1I	GGYRCC	1 cut(s)	892
AciI	CCGC	5 cut(s)	72, 360, 387, 547, 1184
AclI	AACGTT	1 cut(s)	118
AclWI	GGATC	4 cut(s)	627, 641, 654, 746
AcsI	RAATTY	2 cut(s)	266, 380
AcuI	CTGAAG	3 cut(s)	687, 1041, 1245
AcyI	GRCGYC	3 cut(s)	498, 1173, 1200
AgsI	TTSAA	3 cut(s)	877, 919, 955
AhlI	ACTAGT	1 cut(s)	289
AjnI	CCWGG	1 cut(s)	39
AjuI	GAANNNNNNNTTGG	2 cut(s)	68, 100
AluBI	AGCT	6 cut(s)	30, 254, 325, 520, 934, 1045
AluI	AGCT	6 cut(s)	30, 254, 325, 520, 934, 1045
Alw26I	GTCTC	1 cut(s)	503
AlwI	GGATC	4 cut(s)	627, 641, 654, 746
Ama87I	CYCGRG	2 cut(s)	25, 650
AoxI	GGCC	2 cut(s)	416, 654
ApeKI	GCWGC	4 cut(s)	34, 600, 1042, 1088
ApoI	RAATTY	2 cut(s)	266, 380
Asp700I	GAANNNNTTC	1 cut(s)	690
AspLEI	GCGC	1 cut(s)	1235
AspS9I	GGNCC	2 cut(s)	43, 416
AsuC2I	CCSGG	1 cut(s)	1028
AsuHPI	GGTGA	5 cut(s)	374, 401, 809, 851, 994
AsuII	TTCGAA	3 cut(s)	16, 87, 99
AvaI	CYCGRG	2 cut(s)	25, 650
AvaII	GGWCC	1 cut(s)	43
BamHI	GGATCC	1 cut(s)	646
BanI	GGYRCC	1 cut(s)	892
BbsI	GAAGAC	2 cut(s)	108, 1029
BbvI	GCAGC	4 cut(s)	21, 612, 1054, 1100
BccI	CCATC	3 cut(s)	71, 102, 788
BcgI	CGANNNNNNTGC	2 cut(s)	16, 50
BciT130I	CCWGG	1 cut(s)	41
BciVI	GTATCC	1 cut(s)	425
BclI	TGATCA	3 cut(s)	370, 486, 514
BcnI	CCSGG	1 cut(s)	1028
BcoDI	GTCTC	1 cut(s)	503
BcuI	ACTAGT	1 cut(s)	289
BfaI	CTAG	1 cut(s)	290
BfmI	CTRYAG	4 cut(s)	562, 607, 1112, 1262
BfoI	RGCGCY	1 cut(s)	1236
BfuAI	ACCTGC	1 cut(s)	970
BfuI	GTATCC	1 cut(s)	425
BglI	GCCNNNNNGGC	1 cut(s)	1241
BisI	GCNGC	4 cut(s)	35, 601, 1043, 1089
BlsI	GCNGC	4 cut(s)	36, 602, 1044, 1090
Bme1390I	CCNGG	2 cut(s)	41, 1028
Bme18I	GGWCC	1 cut(s)	43
BmeT110I	CYCGRG	2 cut(s)	25, 650
BmgT120I	GGNCC	2 cut(s)	43, 416
BmiI	GGNNCC	3 cut(s)	648, 894, 994
BmrFI	CCNGG	2 cut(s)	41, 1028
BmrI	ACTGGG	1 cut(s)	1130
BmsI	GCATC	8 cut(s)	187, 222, 544, 576, 1066, 1138, 1156, 1171
BmuI	ACTGGG	1 cut(s)	1130
BpiI	GAAGAC	2 cut(s)	108, 1029
BpmI	CTGGAG	1 cut(s)	180
Bpu10I	CCTNAGC	1 cut(s)	930
Bpu14I	TTCGAA	3 cut(s)	16, 87, 99
BpuMI	CCSGG	1 cut(s)	1028
BsaBI	GATNNNNATC	2 cut(s)	369, 618
BsaHI	GRCGYC	3 cut(s)	498, 1173, 1200
BsaJI	CCNNGG	6 cut(s)	39, 75, 651, 738, 1152, 1236
Bse1I	ACTGG	3 cut(s)	140, 601, 1136
Bse8I	GATNNNNATC	2 cut(s)	369, 618
BseBI	CCWGG	1 cut(s)	41
BseDI	CCNNGG	6 cut(s)	39, 75, 651, 738, 1152, 1236
BseGI	GGATG	6 cut(s)	113, 213, 567, 682, 787, 928
BseJI	GATNNNNATC	2 cut(s)	369, 618
BseMII	CTCAG	5 cut(s)	246, 370, 921, 1071, 1236
BseNI	ACTGG	3 cut(s)	140, 601, 1136
BseRI	GAGGAG	3 cut(s)	47, 1133, 1253
BseXI	GCAGC	4 cut(s)	21, 612, 1054, 1100
BshFI	GGCC	2 cut(s)	418, 656
BshNI	GGYRCC	1 cut(s)	892
BsiHKCI	CYCGRG	2 cut(s)	25, 650
BsiSI	CCGG	2 cut(s)	770, 1028
BslFI	GGGAC	1 cut(s)	485
BsmAI	GTCTC	1 cut(s)	503
BsmFI	GGGAC	1 cut(s)	485
BsmI	GAATGC	1 cut(s)	1091
BsnI	GGCC	2 cut(s)	418, 656
BsoBI	CYCGRG	2 cut(s)	25, 650
Bsp119I	TTCGAA	3 cut(s)	16, 87, 99
Bsp143I	GATC	7 cut(s)	370, 486, 514, 619, 646, 751, 1014
BspACI	CCGC	5 cut(s)	72, 360, 387, 547, 1184
BspANI	GGCC	2 cut(s)	418, 656
BspCNI	CTCAG	5 cut(s)	247, 369, 922, 1072, 1237
BspLI	GGNNCC	3 cut(s)	648, 894, 994
BspMAI	CTGCAG	1 cut(s)	1116
BspMI	ACCTGC	1 cut(s)	970
BspPI	GGATC	4 cut(s)	627, 641, 654, 746
BspT104I	TTCGAA	3 cut(s)	16, 87, 99
BspT107I	GGYRCC	1 cut(s)	892
BsrI	ACTGG	3 cut(s)	140, 601, 1136
BssECI	CCNNGG	6 cut(s)	39, 75, 651, 738, 1152, 1236
BssMI	GATC	7 cut(s)	370, 486, 514, 619, 646, 751, 1014
BssNI	GRCGYC	3 cut(s)	498, 1173, 1200
BssT1I	CCWWGG	2 cut(s)	75, 738
Bst2UI	CCWGG	1 cut(s)	41
Bst4CI	ACNGT	5 cut(s)	96, 332, 404, 611, 1263
Bst6I	CTCTTC	2 cut(s)	134, 1221
BstACI	GRCGYC	3 cut(s)	498, 1173, 1200
BstBI	TTCGAA	3 cut(s)	16, 87, 99
BstC8I	GCNNGC	2 cut(s)	32, 1047
BstDEI	CTNAG	6 cut(s)	255, 356, 711, 930, 1080, 1245
BstF5I	GGATG	6 cut(s)	113, 213, 567, 682, 787, 928
BstH2I	RGCGCY	1 cut(s)	1236
BstHHI	GCGC	1 cut(s)	1235
BstKTI	GATC	7 cut(s)	373, 489, 517, 622, 649, 754, 1017
BstMAI	GTCTC	1 cut(s)	503
BstMBI	GATC	7 cut(s)	370, 486, 514, 619, 646, 751, 1014
BstMWI	GCNNNNNNNGC	4 cut(s)	251, 1085, 1181, 1241
BstNI	CCWGG	1 cut(s)	41
BstSCI	CCNGG	2 cut(s)	39, 1026
BstSFI	CTRYAG	4 cut(s)	562, 607, 1112, 1262
BstV1I	GCAGC	4 cut(s)	21, 612, 1054, 1100
BstV2I	GAAGAC	2 cut(s)	108, 1029
BstX2I	RGATCY	3 cut(s)	619, 646, 751
BstYI	RGATCY	3 cut(s)	619, 646, 751
BsuI	GTATCC	1 cut(s)	425
BsuRI	GGCC	2 cut(s)	418, 656
BtgZI	GCGATG	1 cut(s)	49
BtsCI	GGATG	6 cut(s)	113, 213, 567, 682, 787, 928
BtsIMutI	CAGTG	1 cut(s)	616
BveI	ACCTGC	1 cut(s)	970
Cac8I	GCNNGC	2 cut(s)	32, 1047
CfoI	GCGC	1 cut(s)	1235
Cfr13I	GGNCC	2 cut(s)	43, 416
CseI	GACGC	2 cut(s)	1181, 1208
CviAII	CATG	4 cut(s)	314, 409, 527, 1253
DdeI	CTNAG	6 cut(s)	255, 356, 711, 930, 1080, 1245
DpnI	GATC	7 cut(s)	372, 488, 516, 621, 648, 753, 1016
DpnII	GATC	7 cut(s)	370, 486, 514, 619, 646, 751, 1014
Eam1104I	CTCTTC	2 cut(s)	134, 1221
EarI	CTCTTC	2 cut(s)	134, 1221
EciI	GGCGGA	2 cut(s)	61, 536
Eco130I	CCWWGG	2 cut(s)	75, 738
Eco32I	GATATC	1 cut(s)	424
Eco47I	GGWCC	1 cut(s)	43
Eco57I	CTGAAG	3 cut(s)	687, 1041, 1245
Eco88I	CYCGRG	2 cut(s)	25, 650
EcoRI	GAATTC	1 cut(s)	266
EcoRII	CCWGG	1 cut(s)	39
EcoRV	GATATC	1 cut(s)	424
EcoT14I	CCWWGG	2 cut(s)	75, 738
ErhI	CCWWGG	2 cut(s)	75, 738
FaeI	CATG	4 cut(s)	317, 412, 530, 1256
FalI	AAGNNNNNCTT	2 cut(s)	68, 100
FaqI	GGGAC	1 cut(s)	485
FatI	CATG	4 cut(s)	313, 408, 526, 1252
FbaI	TGATCA	3 cut(s)	370, 486, 514
Fnu4HI	GCNGC	4 cut(s)	35, 601, 1043, 1089
FokI	GGATG	6 cut(s)	120, 200, 554, 689, 794, 935
Fsp4HI	GCNGC	4 cut(s)	35, 601, 1043, 1089
FspBI	CTAG	1 cut(s)	290
GlaI	GCGC	1 cut(s)	1234
GluI	GCNGC	4 cut(s)	35, 601, 1043, 1089
GsuI	CTGGAG	1 cut(s)	180
HaeII	RGCGCY	1 cut(s)	1236
HaeIII	GGCC	2 cut(s)	418, 656
HapII	CCGG	2 cut(s)	770, 1028
HgaI	GACGC	2 cut(s)	1181, 1208
HhaI	GCGC	1 cut(s)	1235
Hin1I	GRCGYC	3 cut(s)	498, 1173, 1200
Hin1II	CATG	4 cut(s)	317, 412, 530, 1256
Hin6I	GCGC	1 cut(s)	1233
HinP1I	GCGC	1 cut(s)	1233
HindIII	AAGCTT	1 cut(s)	323
HinfI	GANTC	5 cut(s)	966, 1142, 1193, 1205, 1217
HpaII	CCGG	2 cut(s)	770, 1028
HphI	GGTGA	5 cut(s)	374, 401, 809, 851, 994
Hpy166II	GTNNAC	2 cut(s)	451, 943
Hpy188I	TCNGA	5 cut(s)	133, 375, 965, 1147, 1198
Hpy188III	TCNNGA	7 cut(s)	490, 650, 695, 755, 862, 990, 1012
Hpy8I	GTNNAC	2 cut(s)	451, 943
HpyAV	CCTTC	4 cut(s)	56, 487, 883, 1078
HpyCH4III	ACNGT	5 cut(s)	96, 332, 404, 611, 1263
HpyCH4IV	ACGT	3 cut(s)	118, 498, 1058
HpyCH4V	TGCA	2 cut(s)	1037, 1114
HpyF10VI	GCNNNNNNNGC	4 cut(s)	251, 1085, 1181, 1241
HpyF3I	CTNAG	6 cut(s)	255, 356, 711, 930, 1080, 1245
HpySE526I	ACGT	3 cut(s)	118, 498, 1058
Hsp92I	GRCGYC	3 cut(s)	498, 1173, 1200
Hsp92II	CATG	4 cut(s)	317, 412, 530, 1256
HspAI	GCGC	1 cut(s)	1233
Ksp22I	TGATCA	3 cut(s)	370, 486, 514
Kzo9I	GATC	7 cut(s)	370, 486, 514, 619, 646, 751, 1014
Lsp1109I	GCAGC	4 cut(s)	21, 612, 1054, 1100
LweI	GCATC	8 cut(s)	187, 222, 544, 576, 1066, 1138, 1156, 1171
MaeI	CTAG	1 cut(s)	290
MaeII	ACGT	3 cut(s)	118, 498, 1058
MaeIII	GTNAC	1 cut(s)	351
MalI	GATC	7 cut(s)	372, 488, 516, 621, 648, 753, 1016
MboI	GATC	7 cut(s)	370, 486, 514, 619, 646, 751, 1014
MflI	RGATCY	3 cut(s)	619, 646, 751
MluCI	AATT	5 cut(s)	266, 299, 380, 456, 971
MlyI	GAGTC	2 cut(s)	1187, 1211
MmeI	TCCRAC	1 cut(s)	943
MroXI	GAANNNNTTC	1 cut(s)	690
MseI	TTAA	2 cut(s)	126, 462
MspA1I	CMGCKG	3 cut(s)	254, 360, 1045
MspI	CCGG	2 cut(s)	770, 1028
MspR9I	CCNGG	2 cut(s)	41, 1028
Mva1269I	GAATGC	1 cut(s)	1091
MvaI	CCWGG	1 cut(s)	41
MwoI	GCNNNNNNNGC	4 cut(s)	251, 1085, 1181, 1241
NciI	CCSGG	1 cut(s)	1028
NdeII	GATC	7 cut(s)	370, 486, 514, 619, 646, 751, 1014
NlaIII	CATG	4 cut(s)	317, 412, 530, 1256
NlaIV	GGNNCC	3 cut(s)	648, 894, 994
NmeAIII	GCCGAG	2 cut(s)	1177, 1261
NspV	TTCGAA	3 cut(s)	16, 87, 99
PaeR7I	CTCGAG	1 cut(s)	25
PaqCI	CACCTGC	1 cut(s)	970
PctI	GAATGC	1 cut(s)	1091
PdmI	GAANNNNTTC	1 cut(s)	690
PfeI	GAWTC	3 cut(s)	966, 1142, 1205
PkrI	GCNGC	4 cut(s)	36, 602, 1044, 1090
PleI	GAGTC	2 cut(s)	1187, 1211
PpsI	GAGTC	2 cut(s)	1187, 1211
Psp1406I	AACGTT	1 cut(s)	118
Psp6I	CCWGG	1 cut(s)	39
PspGI	CCWGG	1 cut(s)	39
PspN4I	GGNNCC	3 cut(s)	648, 894, 994
PspPI	GGNCC	2 cut(s)	43, 416
PspXI	VCTCGAGB	1 cut(s)	25
PstI	CTGCAG	1 cut(s)	1116
PsuI	RGATCY	3 cut(s)	619, 646, 751
PvuII	CAGCTG	2 cut(s)	254, 1045
SaqAI	TTAA	2 cut(s)	126, 462
SatI	GCNGC	4 cut(s)	35, 601, 1043, 1089
Sau3AI	GATC	7 cut(s)	370, 486, 514, 619, 646, 751, 1014
Sau96I	GGNCC	2 cut(s)	43, 416
SchI	GAGTC	2 cut(s)	1187, 1211
ScrFI	CCNGG	2 cut(s)	41, 1028
SfaNI	GCATC	8 cut(s)	187, 222, 544, 576, 1066, 1138, 1156, 1171
SfcI	CTRYAG	4 cut(s)	562, 607, 1112, 1262
Sfr274I	CTCGAG	1 cut(s)	25
SfuI	TTCGAA	3 cut(s)	16, 87, 99
SinI	GGWCC	1 cut(s)	43
SlaI	CTCGAG	1 cut(s)	25
SmlI	CTYRAG	1 cut(s)	25
SmoI	CTYRAG	1 cut(s)	25
SpeI	ACTAGT	1 cut(s)	289
Sse9I	AATT	5 cut(s)	266, 299, 380, 456, 971
SsiI	CCGC	5 cut(s)	72, 360, 387, 547, 1184
SspMI	CTAG	1 cut(s)	290
StyD4I	CCNGG	2 cut(s)	39, 1026
StyI	CCWWGG	2 cut(s)	75, 738
TaaI	ACNGT	5 cut(s)	96, 332, 404, 611, 1263
TaiI	ACGT	3 cut(s)	121, 501, 1061
TaqI	TCGA	6 cut(s)	16, 26, 87, 99, 969, 1095
TasI	AATT	5 cut(s)	266, 299, 380, 456, 971
TfiI	GAWTC	3 cut(s)	966, 1142, 1205
Tru1I	TTAA	2 cut(s)	126, 462
Tru9I	TTAA	2 cut(s)	126, 462
TscAI	CASTG	1 cut(s)	616
TseI	GCWGC	4 cut(s)	34, 600, 1042, 1088
TspGWI	ACGGA	2 cut(s)	120, 295
TspRI	CASTG	1 cut(s)	616
VpaK11BI	GGWCC	1 cut(s)	43
XapI	RAATTY	2 cut(s)	266, 380
XhoI	CTCGAG	1 cut(s)	25
XmnI	GAANNNNTTC	1 cut(s)	690
XspI	CTAG	1 cut(s)	290
ZraI	GACGTC	1 cut(s)	499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.