Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0009813

Physical Location & Seq

Genomic Coordinates Forward (+)

43430314 .. 43434467

Gene Structure 4154 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025259

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGGCGTCTAGGGCTCGAAATCCGAACTTGGCATCCTTGATCCTTCTCTCTCTCCTCGCCGTTGCCTCCGCCAAGGTCTTCTTCGAAGAGAAATTTGAAGATGGATGGGAGAAACATTGGGTTAAATCTGACTGGAAGAAAGATGAGAACCTAGCTGGGGAATGGAACTACACCTCTGGCAAATGGAACGGCGACGCTAATGACAAAGGTATCCAAACCAGTGAGGACTACCGGTTCTATGCCATATCAGCTGAGTTCCCTGAATTCAGCAACAAGGATAAAACACTAGTGTTCCAGTTTTCTGTGAAGCATGAACAGAAACTTGACTGTGGTGGTGGCTATATCAAATTGCTCAGTGGTGATGTAGATCAGAAGAAGTTTGGTGGGGATACCCCATACAGCATCATGTTCGGGCCAGATATCTGTGGATACAGCACCAAGAAAGTGCATGCAATTCTCCACTACAACGGAACAAATAAATTGATCAAGAAGGATGTACCTTGTGAGACTGACCAGCTCACTCATGTCTATACCTTTATCATCCGCCCAGATGCTACCTACAGCATCCTTATTGACAATGTAGAGAAGCAAACTGGTAGCCTCTACAGTGACTGGGATCTTCTTCCTCCAAAGAAAATCAAGGATCCCGAAGCCAAGAAGCCTGAAGATTGGGACGACAAGGAATTCATCCCAGACCCTGAAGACAAGAAGCCAGAGGGATATGATGACATTCCCAAGGAAATCTCAGATCCCGAAGCCAAGAAGCCCGAAGATTGGGATGATGAGGAAGATGGTGAATGGACAGCCCCAACCATCCCCAACCCTGAATACAAGGGTGAATGGAGCCCAAAGAAAATTAAGAACCCCAACTACAAGGGAAAGTGGAAGGCACCATTGATTGACAACCCAAAATTCACCGATGACCCTGACCTATATGTTTTCCCCAACTTGAAGTATGTTGGAATTGAATTGTGGCAGGTGAAATCTGGAACTCTGTTTGACAATATCGTGATCACTGATGAGCCGGAGTATGCCAAGCAGCTGGCTGAAGACACATGGGGAAAACAGAAAGATGCTGAGAAAGCAGCATTTGAAGAGGCAGAGAAGATTCAACAGGAAGAGGAGTCCAAGGATGAGCCGTCTGATTCTGATGCTGAGGATGATGAAGATGATGCTGAAGAGGACTCTGATGCTGAATCCAAACTAGAAGAGGCTGAAGAGGCCGTGGAAGAGGCTGCAAAACATGATGAACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005783 GO:0005788 GO:0005911 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0010035 GO:0010038 GO:0012505 GO:0016020 GO:0030054 GO:0031090 GO:0031974 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0055044 GO:0070013 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04141 ko04145 ko04612 ko05142 ko05166 map04141 map04145 map04612 map05142 map05166

Pfam Domains

Protein Families

Calreticulin

Protein Analysis

419

Amino Acids

47.81

Weight (kDa)

4.46

Isoelectric Point (pI)

34.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calreticulin	PF00262	28 - 263	1.6e-59	Calreticulin family
Calreticulin	PF00262	265 - 338	9.5e-22	Calreticulin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009813)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09210 AT1G56340 AT1G56340
fragaria_vesca	FvH4_6g07360
malus_domestica	MD04G1194200.v1.1 MD12G1206700.v1.1
prunus_persica	Prupe.6G313900_v2.0.a1
pyrus_communis	pycom04g17170 pycom12g19460
rosa_chinensis	RchiOBHm_Chr3g0456961
rosa_laevigata	RLG00000025259
rosa_multiflora	Rmu_sc0016008.1_g000004 Rmu_sc0032731.1_g000001
rosa_roxburghii	Rroxscaffold_6G00422330
rosa_rugosa	Rorug03G0017700 Rorug03G0017800
rosa_samantha	Rh3AG077700 Rh3BG079600 Rh3CG079500 Rh3DG081300
rosa_wichuraiana	Rw3G006660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	967
Acc36I	ACCTGC	1 cut(s)	967
AccB1I	GGYRCC	1 cut(s)	889
AciI	CCGC	2 cut(s)	69, 544
AclWI	GGATC	5 cut(s)	34, 624, 638, 651, 743
AcsI	RAATTY	4 cut(s)	92, 263, 683, 911
AcuI	CTGAAG	5 cut(s)	684, 720, 1068, 1197, 1236
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	1 cut(s)	498
AfiI	CCNNNNNNNGG	2 cut(s)	157, 774
AgeI	ACCGGT	1 cut(s)	231
AgsI	TTSAA	5 cut(s)	98, 952, 968, 1094, 1112
AhlI	ACTAGT	1 cut(s)	286
AjuI	GAANNNNNNNTTGG	2 cut(s)	65, 97
AloI	GAACNNNNNNTCC	4 cut(s)	17, 49, 218, 250
AluBI	AGCT	4 cut(s)	155, 251, 517, 1042
AluI	AGCT	4 cut(s)	155, 251, 517, 1042
Alw26I	GTCTC	1 cut(s)	500
AlwI	GGATC	5 cut(s)	34, 624, 638, 651, 743
AlwNI	CAGNNNCTG	2 cut(s)	612, 698
AoxI	GGCC	2 cut(s)	413, 1221
ApeKI	GCWGC	3 cut(s)	1039, 1085, 1235
ApoI	RAATTY	4 cut(s)	92, 263, 683, 911
AsiGI	ACCGGT	1 cut(s)	231
AspS9I	GGNCC	1 cut(s)	413
AsuHPI	GGTGA	5 cut(s)	371, 806, 848, 907, 991
AsuII	TTCGAA	1 cut(s)	84
BaeI	ACNNNNGTAYC	2 cut(s)	193, 226
BamHI	GGATCC	1 cut(s)	643
BanI	GGYRCC	1 cut(s)	889
BanII	GRGCYC	2 cut(s)	16, 848
BbsI	GAAGAC	3 cut(s)	70, 708, 1056
BbvCI	CCTCAGC	1 cut(s)	1155
BbvI	GCAGC	3 cut(s)	1051, 1097, 1222
BccI	CCATC	4 cut(s)	95, 99, 785, 821
BceAI	ACGGC	4 cut(s)	44, 205, 1123, 1208
BcgI	CGANNNNNNTGC	2 cut(s)	391, 425
BciVI	GTATCC	3 cut(s)	221, 382, 422
BclI	TGATCA	2 cut(s)	483, 1011
BcoDI	GTCTC	1 cut(s)	500
BcuI	ACTAGT	1 cut(s)	286
BfaI	CTAG	4 cut(s)	9, 152, 287, 1205
BfmI	CTRYAG	3 cut(s)	559, 604, 1253
BfuAI	ACCTGC	1 cut(s)	967
BfuI	GTATCC	3 cut(s)	221, 382, 422
BisI	GCNGC	3 cut(s)	1040, 1086, 1236
BlsI	GCNGC	3 cut(s)	1041, 1087, 1237
BmgT120I	GGNCC	1 cut(s)	413
BmiI	GGNNCC	3 cut(s)	645, 845, 891
BmrI	ACTGGG	1 cut(s)	622
BmsI	GCATC	8 cut(s)	41, 411, 541, 573, 1063, 1141, 1162, 1180
BmuI	ACTGGG	1 cut(s)	622
BpiI	GAAGAC	3 cut(s)	70, 708, 1056
Bpu10I	CCTNAGC	1 cut(s)	1155
Bpu14I	TTCGAA	1 cut(s)	84
BsaBI	GATNNNNATC	1 cut(s)	366
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	4 cut(s)	72, 735, 1128, 1224
BsaWI	WCCGGW	1 cut(s)	231
Bsc4I	CCNNNNNNNGG	2 cut(s)	157, 774
Bse118I	RCCGGY	1 cut(s)	231
Bse1I	ACTGG	5 cut(s)	137, 219, 295, 598, 617
Bse8I	GATNNNNATC	1 cut(s)	366
BseDI	CCNNGG	4 cut(s)	72, 735, 1128, 1224
BseJI	GATNNNNATC	1 cut(s)	366
BseLI	CCNNNNNNNGG	2 cut(s)	157, 774
BseMII	CTCAG	5 cut(s)	243, 367, 759, 1068, 1146
BseNI	ACTGG	5 cut(s)	137, 219, 295, 598, 617
BseRI	GAGGAG	2 cut(s)	44, 1136
BseXI	GCAGC	3 cut(s)	1051, 1097, 1222
BseYI	CCCAGC	1 cut(s)	155
BshFI	GGCC	2 cut(s)	415, 1223
BshNI	GGYRCC	1 cut(s)	889
BshTI	ACCGGT	1 cut(s)	231
BsiSI	CCGG	2 cut(s)	232, 1025
BslFI	GGGAC	1 cut(s)	686
BslI	CCNNNNNNNGG	2 cut(s)	157, 774
BsmAI	GTCTC	1 cut(s)	500
BsmFI	GGGAC	1 cut(s)	686
BsnI	GGCC	2 cut(s)	415, 1223
Bsp119I	TTCGAA	1 cut(s)	84
Bsp1286I	GDGCHC	2 cut(s)	16, 848
Bsp143I	GATC	7 cut(s)	39, 367, 483, 616, 643, 748, 1011
BspACI	CCGC	2 cut(s)	69, 544
BspANI	GGCC	2 cut(s)	415, 1223
BspCNI	CTCAG	5 cut(s)	244, 366, 758, 1069, 1147
BspLI	GGNNCC	3 cut(s)	645, 845, 891
BspMI	ACCTGC	1 cut(s)	967
BspPI	GGATC	5 cut(s)	34, 624, 638, 651, 743
BspT104I	TTCGAA	1 cut(s)	84
BspT107I	GGYRCC	1 cut(s)	889
BsrFI	RCCGGY	1 cut(s)	231
BsrI	ACTGG	5 cut(s)	137, 219, 295, 598, 617
BssAI	RCCGGY	1 cut(s)	231
BssECI	CCNNGG	4 cut(s)	72, 735, 1128, 1224
BssMI	GATC	7 cut(s)	39, 367, 483, 616, 643, 748, 1011
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	3 cut(s)	72, 735, 1128
Bst4CI	ACNGT	3 cut(s)	329, 608, 1254
Bst6I	CTCTTC	7 cut(s)	81, 1089, 1113, 1173, 1203, 1212, 1224
BstACI	GRCGYC	1 cut(s)	5
BstBI	TTCGAA	1 cut(s)	84
BstC8I	GCNNGC	2 cut(s)	450, 1044
BstDEI	CTNAG	5 cut(s)	252, 353, 745, 1077, 1155
BstDSI	CCRYGG	1 cut(s)	1224
BstKTI	GATC	7 cut(s)	42, 370, 486, 619, 646, 751, 1014
BstMAI	GTCTC	1 cut(s)	500
BstMBI	GATC	7 cut(s)	39, 367, 483, 616, 643, 748, 1011
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 248, 1082, 1220
BstNSI	RCATGY	1 cut(s)	452
BstSFI	CTRYAG	3 cut(s)	559, 604, 1253
BstV1I	GCAGC	3 cut(s)	1051, 1097, 1222
BstV2I	GAAGAC	3 cut(s)	70, 708, 1056
BstX2I	RGATCY	3 cut(s)	616, 643, 748
BstXI	CCANNNNNNTGG	1 cut(s)	1042
BstYI	RGATCY	3 cut(s)	616, 643, 748
BsuI	GTATCC	3 cut(s)	221, 382, 422
BsuRI	GGCC	2 cut(s)	415, 1223
BtgI	CCRYGG	1 cut(s)	1224
BtsIMutI	CAGTG	4 cut(s)	226, 361, 613, 1014
BveI	ACCTGC	1 cut(s)	967
Cac8I	GCNNGC	2 cut(s)	450, 1044
CaiI	CAGNNNCTG	2 cut(s)	612, 698
Cfr10I	RCCGGY	1 cut(s)	231
Cfr13I	GGNCC	1 cut(s)	413
CseI	GACGC	1 cut(s)	203
Csp6I	GTAC	1 cut(s)	497
CspAI	ACCGGT	1 cut(s)	231
CviAII	CATG	6 cut(s)	311, 406, 449, 524, 1056, 1244
CviQI	GTAC	1 cut(s)	497
DdeI	CTNAG	5 cut(s)	252, 353, 745, 1077, 1155
DpnI	GATC	7 cut(s)	41, 369, 485, 618, 645, 750, 1013
DpnII	GATC	7 cut(s)	39, 367, 483, 616, 643, 748, 1011
Eam1104I	CTCTTC	7 cut(s)	81, 1089, 1113, 1173, 1203, 1212, 1224
EarI	CTCTTC	7 cut(s)	81, 1089, 1113, 1173, 1203, 1212, 1224
EciI	GGCGGA	2 cut(s)	58, 533
Eco130I	CCWWGG	3 cut(s)	72, 735, 1128
Eco24I	GRGCYC	2 cut(s)	16, 848
Eco32I	GATATC	1 cut(s)	421
Eco57I	CTGAAG	5 cut(s)	684, 720, 1068, 1197, 1236
EcoRI	GAATTC	2 cut(s)	263, 683
EcoRV	GATATC	1 cut(s)	421
EcoT14I	CCWWGG	3 cut(s)	72, 735, 1128
EcoT38I	GRGCYC	2 cut(s)	16, 848
ErhI	CCWWGG	3 cut(s)	72, 735, 1128
FaeI	CATG	6 cut(s)	314, 409, 452, 527, 1059, 1247
FalI	AAGNNNNNCTT	2 cut(s)	65, 97
FaqI	GGGAC	1 cut(s)	686
FatI	CATG	6 cut(s)	310, 405, 448, 523, 1055, 1243
FbaI	TGATCA	2 cut(s)	483, 1011
Fnu4HI	GCNGC	3 cut(s)	1040, 1086, 1236
FriOI	GRGCYC	2 cut(s)	16, 848
Fsp4HI	GCNGC	3 cut(s)	1040, 1086, 1236
FspBI	CTAG	4 cut(s)	9, 152, 287, 1205
GluI	GCNGC	3 cut(s)	1040, 1086, 1236
GsaI	CCCAGC	1 cut(s)	159
HaeIII	GGCC	2 cut(s)	415, 1223
HapII	CCGG	2 cut(s)	232, 1025
HgaI	GACGC	1 cut(s)	203
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	6 cut(s)	314, 409, 452, 527, 1059, 1247
HinfI	GANTC	5 cut(s)	1108, 1124, 1145, 1184, 1196
HpaII	CCGG	2 cut(s)	232, 1025
HphI	GGTGA	5 cut(s)	371, 806, 848, 907, 991
Hpy188I	TCNGA	7 cut(s)	24, 130, 372, 748, 1144, 1150, 1189
Hpy188III	TCNNGA	5 cut(s)	487, 647, 752, 987, 1009
Hpy99I	CGWCG	1 cut(s)	197
HpyAV	CCTTC	3 cut(s)	53, 484, 880
HpyCH4III	ACNGT	3 cut(s)	329, 608, 1254
HpyCH4V	TGCA	3 cut(s)	448, 452, 1238
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 248, 1082, 1220
HpyF3I	CTNAG	5 cut(s)	252, 353, 745, 1077, 1155
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	6 cut(s)	314, 409, 452, 527, 1059, 1247
Ksp22I	TGATCA	2 cut(s)	483, 1011
Kzo9I	GATC	7 cut(s)	39, 367, 483, 616, 643, 748, 1011
LmnI	GCTCC	1 cut(s)	843
Lsp1109I	GCAGC	3 cut(s)	1051, 1097, 1222
LweI	GCATC	8 cut(s)	41, 411, 541, 573, 1063, 1141, 1162, 1180
MaeI	CTAG	4 cut(s)	9, 152, 287, 1205
MaeIII	GTNAC	1 cut(s)	608
MalI	GATC	7 cut(s)	41, 369, 485, 618, 645, 750, 1013
MboI	GATC	7 cut(s)	39, 367, 483, 616, 643, 748, 1011
MflI	RGATCY	3 cut(s)	616, 643, 748
MhlI	GDGCHC	2 cut(s)	16, 848
MlyI	GAGTC	2 cut(s)	1133, 1178
MmeI	TCCRAC	1 cut(s)	940
MseI	TTAA	2 cut(s)	123, 858
MspA1I	CMGCKG	2 cut(s)	251, 1042
MspI	CCGG	2 cut(s)	232, 1025
MwoI	GCNNNNNNNGC	4 cut(s)	11, 248, 1082, 1220
NdeII	GATC	7 cut(s)	39, 367, 483, 616, 643, 748, 1011
NlaIII	CATG	6 cut(s)	314, 409, 452, 527, 1059, 1247
NlaIV	GGNNCC	3 cut(s)	645, 845, 891
NmuCI	GTSAC	1 cut(s)	608
NspI	RCATGY	1 cut(s)	452
NspV	TTCGAA	1 cut(s)	84
PaeI	GCATGC	1 cut(s)	452
PaqCI	CACCTGC	1 cut(s)	967
PfeI	GAWTC	3 cut(s)	1108, 1145, 1196
PinAI	ACCGGT	1 cut(s)	231
PkrI	GCNGC	3 cut(s)	1041, 1087, 1237
PleI	GAGTC	2 cut(s)	1132, 1178
PpsI	GAGTC	2 cut(s)	1132, 1178
PspFI	CCCAGC	1 cut(s)	155
PspN4I	GGNNCC	3 cut(s)	645, 845, 891
PspPI	GGNCC	1 cut(s)	413
PsrI	GAACNNNNNNTAC	2 cut(s)	854, 886
PstNI	CAGNNNCTG	2 cut(s)	612, 698
PsuI	RGATCY	3 cut(s)	616, 643, 748
PvuII	CAGCTG	2 cut(s)	251, 1042
RsaI	GTAC	1 cut(s)	498
RsaNI	GTAC	1 cut(s)	497
SaqAI	TTAA	2 cut(s)	123, 858
SatI	GCNGC	3 cut(s)	1040, 1086, 1236
Sau3AI	GATC	7 cut(s)	39, 367, 483, 616, 643, 748, 1011
Sau96I	GGNCC	1 cut(s)	413
SchI	GAGTC	2 cut(s)	1133, 1178
SduI	GDGCHC	2 cut(s)	16, 848
SfaNI	GCATC	8 cut(s)	41, 411, 541, 573, 1063, 1141, 1162, 1180
SfcI	CTRYAG	3 cut(s)	559, 604, 1253
SfuI	TTCGAA	1 cut(s)	84
SpeI	ACTAGT	1 cut(s)	286
SphI	GCATGC	1 cut(s)	452
SsiI	CCGC	2 cut(s)	69, 544
SspMI	CTAG	4 cut(s)	9, 152, 287, 1205
StyI	CCWWGG	3 cut(s)	72, 735, 1128
TaaI	ACNGT	3 cut(s)	329, 608, 1254
TaqI	TCGA	2 cut(s)	16, 84
TfiI	GAWTC	3 cut(s)	1108, 1145, 1196
Tru1I	TTAA	2 cut(s)	123, 858
Tru9I	TTAA	2 cut(s)	123, 858
TscAI	CASTG	4 cut(s)	226, 361, 613, 1021
TseFI	GTSAC	1 cut(s)	608
TseI	GCWGC	3 cut(s)	1039, 1085, 1235
Tsp45I	GTSAC	1 cut(s)	608
TspDTI	ATGAA	3 cut(s)	327, 676, 1179
TspGWI	ACGGA	1 cut(s)	483
TspRI	CASTG	4 cut(s)	226, 361, 613, 1021
XapI	RAATTY	4 cut(s)	92, 263, 683, 911
XceI	RCATGY	1 cut(s)	452
XspI	CTAG	4 cut(s)	9, 152, 287, 1205

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.